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A PTK7/Ror2 Co-Receptor Complex Affects Xenopus Neural Crest Migration

  • Martina Podleschny ,

    Contributed equally to this work with: Martina Podleschny, Anita Grund

    Affiliation Faculty of Biology, Molecular Embryology, Philipps-Universität Marburg, 35043 Marburg, Germany

  • Anita Grund ,

    Contributed equally to this work with: Martina Podleschny, Anita Grund

    Affiliation Faculty of Biology, Molecular Embryology, Philipps-Universität Marburg, 35043 Marburg, Germany

  • Hanna Berger,

    Affiliation Faculty of Biology, Molecular Embryology, Philipps-Universität Marburg, 35043 Marburg, Germany

  • Erik Rollwitz,

    Affiliation Faculty of Biology, Molecular Embryology, Philipps-Universität Marburg, 35043 Marburg, Germany

  • Annette Borchers

    borchers@uni-marburg.de

    Affiliation Faculty of Biology, Molecular Embryology, Philipps-Universität Marburg, 35043 Marburg, Germany

A PTK7/Ror2 Co-Receptor Complex Affects Xenopus Neural Crest Migration

  • Martina Podleschny, 
  • Anita Grund, 
  • Hanna Berger, 
  • Erik Rollwitz, 
  • Annette Borchers
PLOS
x

Abstract

Neural crest cells are a highly migratory pluripotent cell population that generates a wide array of different cell types and failure in their migration can result in severe birth defects and malformation syndromes. Neural crest migration is controlled by various means including chemotaxis, repellent guidance cues and cell-cell interaction. Non-canonical Wnt PCP (planar cell polarity) signaling has previously been shown to control cell-contact mediated neural crest cell guidance. PTK7 (protein tyrosine kinase 7) is a transmembrane pseudokinase and a known regulator of Wnt/PCP signaling, which is expressed in Xenopus neural crest cells and required for their migration. PTK7 functions as a Wnt co-receptor; however, it remains unclear by which means PTK7 affects neural crest migration. Expressing fluorescently labeled proteins in Xenopus neural crest cells we find that PTK7 co-localizes with the Ror2 Wnt-receptor. Further, co-immunoprecipitation experiments demonstrate that PTK7 interacts with Ror2. The PTK7/Ror2 interaction is likely relevant for neural crest migration, because Ror2 expression can rescue the PTK7 loss of function migration defect. Live cell imaging of explanted neural crest cells shows that PTK7 loss of function affects the formation of cell protrusions as well as cell motility. Co-expression of Ror2 can rescue these defects. In vivo analysis demonstrates that a kinase dead Ror2 mutant cannot rescue PTK7 loss of function. Thus, our data suggest that Ror2 can substitute for PTK7 and that the signaling function of its kinase domain is required for this effect.

Introduction

Neural crest (NC) cells are a highly migratory pluripotent cell population that gives rise to a wide range of derivatives contributing to many tissues and organs. NC cells develop along the anterior-posterior axis of the vertebrate embryo at the border region between the epidermis and the neural plate. After undergoing an epithelial to mesenchymal transition NC cells migrate and colonize almost all tissues of the embryo. In this respect NC migration is very similar to cancer cell invasion and metastasis dissemination, which is also mirrored by the conservation of molecules and signaling pathways involved in both processes [1,2,3]. During migration NC cells follow precise pathways and encounter various molecular microenvironments, which guide them to their final destinations and determine their terminal differentiation [4]. Therefore, NC migration needs to be tightly controlled to ensure the development of multiple organs and tissues. Indeed, failure results in severe birth defects and malformation syndromes–so called neurocristopathies [5,6,7]. Thus, understanding the molecular mechanism that control NC migration will also provide insight into pathological conditions like neurocristopathies or the development of cancer.

Non-canonical– ß-catenin-independent–Wnt signaling contributes significantly to the regulation of NC migration [8,9,10,11,12,13,14]. NC cells show collective cell migration and form streams of migrating cells directed for example by repellent guidance cues and chemo-attractants [2,4]. In addition, NC cell directionality is achieved by communication of NC cells with each other. One mechanism, which was proposed for the directional migration of cranial NC cells is contact inhibition of locomotion. This phenomenon was discovered by Abercrombie [15,16] and describes how two colliding cells change their cell polarization upon cell-cell-contact and migrate in opposite directions. Recent work showed that non-canonical planar cell polarity (PCP) Wnt signaling is required for contact inhibition of locomotion and directional collective NC migration [8,9,10,17,18]. PCP signaling was first discovered in the fly where it regulates the orientation of hairs in the wing or ommatidia organization in the eye [19,20]. In vertebrates PCP signaling is necessary for inner ear patterning, ciliary beating and tissue movements contributing to axis elongation and neural tube closure [21,22]. In all these biological systems PCP proteins become asymmetrically localized to the plasma membrane thereby establishing a polarity in the plane of an epithelium. In moving tissues the situation is more complicated. In migrating NC cells PCP signaling seems to determine the formation of cellular protrusions by asymmetrically regulating the activity of small GTPases of the Rho family [14,18,23]. PCP proteins including Frizzled and Dishevelled are localized to the site of cell contact thereby leading to a local activation of Rho and inhibition of Rac [8,9,17,24]. Thus, cell protrusions collapse and cells migrate in opposite directions. Thereby, PCP signaling provides a means of NC cell dispersion, which in combination with other repellent, attractant and adhesive cues contributes to controlled migration of NC cells [23,25,26,27]. Although, the contribution of PCP signaling to NC migration has been acknowledged in different vertebrate systems the molecular components localizing to cell-contact sites and the signaling pathways leading to cytoskeletal remodeling remain to be characterized.

Protein tyrosine kinase 7 (PTK7, also known as Colon Carcinoma Kinase-4, CCK-4) a known regulator of planar cell polarity may be one of the players involved in cell-contact-mediated NC cell guidance. PTK7 is an evolutionary conserved transmembrane protein with extracellular immunoglobulin domains and an intracellular kinase homology domain, which lacks catalytic activity [28,29]. In Xenopus PTK7 is required for NC migration [13]. As PTK7 interacts with Frizzled7 and recruits Dishevelled to the plasma membrane [13,30], it could play a role in cell-contact-mediated NC cell guidance. Recent publications suggest that PTK7 affects multiple Wnt signaling pathways. Loss of function analysis in mice, zebrafish and Xenopus show that PTK7 is required for non-canonical Wnt signaling controlling convergent extension cell movements and planar cell polarity [31,32,33,34]. In addition, PTK7 function has been implicated in canonical Wnt signaling. Recently, we and others demonstrated that PTK7 interacts with canonical Wnt proteins [35,36] and inhibits canonical Wnt signaling [34,35]. Contradictory to these findings a role of PTK7 as an active component of canonical Wnt signaling has also been reported [37,38] and PTK7 was shown to interact with LRP6 [38]. Therefore, differences in the composition of the PTK7 co-receptor complex may explain these contradictory findings in different tissue settings. This is also supported by reports concerning PTK7 downstream signaling, where various signaling outcomes like activation of c-jun, AKT, Ras/ERK, CREB/ATF1, SRC and inhibition of JNK signaling were observed [39,40,41,42]. The fact that PTK7 interacts with other receptors like Plexin and VEGF receptors further supports this hypothesis and suggests that PTK7 is a versatile co-receptor [43]. Similarly, receptor context may also modulate PTK7 function in Wnt signaling, however, so far interaction with non-canonical Wnt receptors such as Ror2 have not been analyzed.

The receptor-tyrosine kinase Ror2 is an evolutionary conserved Wnt receptor that has been shown to activate ß-catenin-independent Wnt pathways and to antagonize Wnt/ß-catenin signaling [44,45,46,47,48,49,50]. The extracellular part of the Ror2 protein contains one Ig-domain, the Wnt-binding CRD domain, and one kringle domain; its intracellular part consists of a supposedly active tyrosine-kinase domain and a C-terminal serine/threonine- and proline-rich domain. The latter is the major interface for cytoplasmic interactions e.g. with Dishevelled, Filamin A, CK1 and GSK3ß [51,52,53,54]. In vertebrates, Ror2 was found to form a receptor-complex with Frizzled and is one major co-receptor in Wnt/Frizzled mediated planar cell polarity signaling [55,56] and Wnt-regulated cell movements [52,57,58,59]. In addition, Ror2 can act as a bona fide receptor tyrosine kinase to activate a PI3K-JNK cascade in gastrulating Xenopus embryos [44]. Ror2 is expressed in the dorsal mesoderm, the neuroectoderm, in pre-migratory and migrating NC cells in Xenopus embryos and in the cranial ganglia of tadpoles [57,60]. Thus, Ror2 and PTK7 are co-expressed in migrating NC cells and share a common function in the regulation of canonical and non-canonical Wnt signaling pathways suggesting that they may interact in the regulation of NC migration. Here, we provide evidence supporting this hypothesis. We find that PTK7 and Ror2 co-localize in NC cells and that they interact biochemically. Further, Ror2 can rescue the PTK7 loss of function NC migration defect indicating that it can substitute for PTK7.

Materials and Methods

Plasmid cloning

For cloning of the HA-tagged full-length mouse Ror2 (Ror2-HA) the coding sequence of mouse ror2 was amplified using the following primers: forward 5’ACGCTCGAGGTGCATCGGGGCAGGAAAGGGGAC3’ and reverse 5’ACGCTCGAGGGCTTCAAGCTGGACATGAGCCG3’. The PCR product was introduced into the XhoI restriction site of the pCS2+/HA vector. For myc-tagged full-length human PTK7 (PTK7-MT), the coding sequence of human ptk7 was amplified using the following primers: forward 5’CACGTGATCGATGCCCTCAGCTCCTTTTCCTGA3’ and reverse 5’GACGTGATCGATGCGGCTTGCTGTCCACGGT3’. The PCR product was introduced into the ClaI restriction site of pCS2+/MT. Myc-tagged human PTK7 lacking the intracellular kinase homology domain (h ΔkPTK7-MT) was amplified from PTK7-MT using the following primers: forward 5’CACGTGATCGATGCCCTCAGCTCCTTTTCCTGA3’ and reverse 5’CCGTATCGATCGGGCTCCTTCTGCAGC3’. The PCR product was introduced into the ClaI restriction site of pCS2+/MT. For the secreted extracellular domain of mouse Ror2 (sRor2-HA) the extra cellular part was amplified using the following primers: forward 5’CAGCTCGAGCCGCAGCATGGCTCGGGGCTGG3’ and reverse 5’gctgCTCGAG acacgggggtacgtcacacagttg3’. The PCR product was introduced into the XhoI site of pCS2+/HA. The Xenopus RFP-tagged PTK7 (PTK7-RFP) was cloned using a 3 step PCR with a pair of chimeric primers thereby fusing the RFP-tag in frame to the C-terminus of PTK7.

Xenopus injection

Xenopus embryos were generated and cultured according to general protocols and staged according to the normal table of Nieuwkoop and Faber [61]. All procedures were performed according to the German animal use and care law (Tierschutzgesetz) and approved by the German state administration Hesse (Regierungspräsidium Giessen). For Xenopus microinjection capped sense mRNA was synthesized using the mMessage Machine Kit (Ambion, Life Technologies). The following published plasmids were used: LacZ [62], mGFP [63], H2B-mcherry [64], Xenopus Ror2-3I [57], mouse Ror2∆469-Flag [65] and Xenopus Ror2-EGFP [60].

For the PTK7 loss of function rescue experiments different combinations of MO and sense mRNA were co-injected. 10 ng of PTK7 MO (a combination of two different MO was used as previously described [30]) or standard control MO (GeneTools) were co-injected with 100 pg mGFP, mouse Ror2, mouse Ror2Δ469 or Xenopus Ror2-3I in one blastomere of a two-cell stage embryo. In addition 100 pg LacZ RNA was injected as a lineage tracer. Cultivation, staging and fixation of embryos were performed as previously described [66]. Embryos were cultured until tadpole stages (stage 26–28) and then further analyzed using ß-galactosidase staining and in situ hybridization using the NC marker twist.

Cranial NC explants and time-lapse imaging

For NC cell explantation embryos were microinjected with 50 pg mGFP mRNA and 250 pg H2B-mcherry mRNA together with 7.5 ng control MO or PTK7 MO in one blastomere at the two-cell stage; for rescue experiments 150 pg Ror2 RNA were co-injected. To analyze for co-localization of PTK7 and Ror2 embryos were injected with 400 pg PTK7-RFP and 150 pg Ror2-EGFP. Explantation of NC cells was performed as described [67]. Explanted NC cells were placed in fibronectin coated (1 mg/ml in PBS) chamber slides (Lab-Tek Chambered Coverglass (Thermo Scientific)) filled with DFA medium (53 mM NaCl, 5 mM Na2CO3, 4.5 mM K-Gluconacid, 32 mM Na-Gluconacid, 1 mM MgSO4, 1 mM CaCl2, 0.1% BSA, adjusted with bicine (1M) to pH 8.3). Explants were dissected into smaller pieces using an eyebrow-knife and incubated for two to four hours at 14°C to ensure adherence of the cells. Subsequently NC migration was observed and analyzed by spinning disk microscopy (AxioObserver Z1, Zeiss, Objective 10x plan apochromat NA 0,45 or 63x plan apochromat NA 1.4 oil). Images were taken every 30 seconds (for imaging at 63x) or every 60 seconds (for imaging at 10x) for a time interval of up to 8 hours.

Image analysis was performed using ImageJ. For cell tracking the MTrackJ plugin [68] was used. Briefly, for each condition 5 cells were tracked using the nuclear H2B fluorescence to follow their movement throughout all frames of the movie. Further, to analyze the dispersion of NC cells Delaunay triangulation was used. In short, the nuclei were selected and the Delaunay/Voronoi diagram plugin was used to draw a Delaunay diagram from the point selections.

Co-immunoprecipitation and Western blot

For co-immunoprecipitation experiments MCF7 cells were transfected using Lipofectamine 2000™ (Life Technologies) with the following plasmids: Ror2ΔCRD/KR∆745-Flag, Ror2Δ745-Flag and Ror2∆469-Flag [65], Ror2-HA, sRor2-HA, PTK7-MT and ∆kPTK7-MT. In case of single plasmid transfection 2 μg of pCS2+ vector without insert were added to equalize plasmid concentrations. 48 hours after transfection cells were washed in TBS (50 mM Tris (pH 7.5), 150 mM NaCl), scraped and lysed in lysis buffer (TBS containing 0.5% NP-40 and Complete protease inhibitor mix EDTA-free (Roche)). 750 μl of total lysate were pre-cleared by continuous mixing with Protein A Sepharose CL-4B (GE Healthcare) for one hour at 4°C. After centrifugation 50 μl of the pre-cleared lysate were taken as input control. For co-immunoprecipitation mouse anti-HA (HA.11 16B12 Covance, dilution 1:150), mouse anti-myc (9E10 M5546 Sigma, dilution 1:500), goat anti-myc (ab 19234 Abcam, dilution 1:250) or mouse anti-Flag M2 antibodies (F4049 Sigma, dilution 1:500) were added to the supernatants and incubated at 4°C for two hours. Subsequently Protein A Sepharose slurry was added and probes were further incubated at 4°C for one hour. The Protein A Sepharose beads were washed five times with lysis buffer for five minutes at 4°C. The beads were mixed with 6x Laemmli loading buffer (0.35 M Tris (pH 6.8), 30% Glycerin, 10% SDS, 9.3% Dithiothreitol, 0.02% bromphenol blue), denatured at 95°C and loaded on 10% SDS PAGE gels. Detection of proteins by immunoblotting was carried out using different antibodies: goat anti-myc (ab 19234 Abcam, dilution 1:1000), mouse anti-myc (9E10 M5546 Sigma, dilution 1:2000), mouse anti-HA (HA.11 16B12 Covance, dilution 1:1000), goat anti-Ror2 (AF 2064 R&D Systems, dilution 1:1000). As secondary antibodies anti-mouse HRP (sc2005 Santa Cruz, dilution 1:5000) or anti-goat HRP (sc2020 Santa Cruz, dilution 1:10000) were used.

Results

PTK7 and Ror2 co-localize in NC cells and interact in co-immunoprecipitation experiments

Published data suggest that PTK7 is a versatile co-receptor, however, its interaction partners in migrating NC cells have not been determined yet. To analyze if PTK7 may interact with the Ror2 receptor, we first checked for co-localization in migrating NC cells. RFP-labeled PTK7 and GFP-labeled Ror2 were expressed in Xenopus NC cells, which were explanted at premigratory stage 17 and monitored using live-cell imaging. Interestingly, distinct areas of the cell membrane could be distinguished where either PTK7, Ror2 or a combination of both proteins were localized (Fig 1A). The fact that PTK7 and Ror2 co-localize at the cell membrane suggests that these proteins may form a co-receptor complex.

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Fig 1. PTK7 and Ror2 co-localize in NC cells and co-precipitate independent of the kinase-homology domain of PTK7.

A Co-localization of PTK7 and Ror2. NC cells co-expressing Ror2-EGFP and PTK7-RFP show distinct areas of co-localization (yellow) as well as membrane areas where only PTK7-RFP (red) or Ror2-EGFP (green) is localized. The right panel shows a higher magnification of the single cell in the left panel (indicated by a dashed square), scale bar = 10 μm. B,C Co-immunoprecipitation of PTK7 and Ror2. Full-length myc-tagged PTK7 (PTK7-MT) and a myc-tagged PTK7 deletion construct lacking the kinase homology domain (∆kPTK7-MT) as well as a full-length HA-tagged Ror2 construct were expressed in MCF7 cells. B Constructs and their protein domains are depicted in the top panel. Abbreviations are as follows: IG (immunoglobulin domain), CRD (cysteine-rich domain), K (kringle domain), TM (transmembrane domain), KH (kinase homology domain), TK (tyrosine kinase), S/T (serine/threonine-rich domain), P (proline-rich domain). C Immunoprecipitation experiment; the cell transfection scheme is indicated at the top. Co-immunoprecipitation was carried out using either anti-myc (IP α-MT, upper panel) or anti-HA antibodies (IP α-HA, middle panel). The respective cell lysates are shown in the bottom panel. Antibodies used for Western blotting and molecular weights are indicated at the right.

https://doi.org/10.1371/journal.pone.0145169.g001

In order to determine a potential biochemical interaction between PTK7 and Ror2 we tested for co-immunoprecipitation. Full-length myc-tagged PTK7 (PTK7-MT) and full-length HA-tagged Ror2 (Ror2-HA) were overexpressed in MCF7 cells. Precipitation of PTK7 using myc-antibodies resulted in co-precipitation of Ror2, while EGFR and TGFßR1 were not co-precipitated (Fig 1B and 1C; S1 Fig). Conversely, precipitation of Ror2 using HA-antibodies co-precipitated PTK7 (Fig 1C), but not EGFR or TGFßR1 (S1 Fig). As the intracellular kinase-homology domain of PTK7 is also required for its function [13,30], we further tested if full-length Ror2 also interacts with a PTK7 construct lacking this domain (∆kPTK7-HA). Indeed, we found that Ror2 co-precipitates ∆kPTK7 and vice versa (Fig 1C). Thus, these data suggest that PTK7 and Ror2 interact and that this interaction does not require the kinase homology domain of PTK7.

To determine which Ror2 domains are required for PTK7/Ror2 interaction different Ror2 deletion mutants were used (Fig 2A). Ror2Δ745 has a deletion of the intracellular serine/threonine-rich domains. The Ror2ΔCRD/KR∆745 mutant lacks additionally the extracellular frizzled-like cysteine-rich domain (CRD) required for Wnt binding and the kringle domain (KR). In the Ror2Δ469 construct major parts of the intracellular domain including the tyrosine-kinase domain, the serine/threonine-rich and the proline-rich domains have been deleted. Finally, the sRor2 lacks the entire intracellular and transmembrane domains. The FLAG-tagged Ror2 deletion constructs were co-expressed with full-length myc-tagged PTK7 and co-precipitations were performed using anti-FLAG antibodies. All Ror2 deletion mutants were able to co-precipitate full-length PTK7 (Fig 2B and 2C and data not shown). Conversely, if PTK7 was precipitated using anti-myc antibodies sRor2 was co-precipitated (Fig 2C). Thus, the PTK7/Ror2 interaction is likely mediated by the extracellular domains of these proteins, but does not require the CRD and kringle domains of Ror2.

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Fig 2. The intracellular domain and the CRD domain of Ror2 are not required for PTK7/Ror2 interaction.

Myc-tagged PTK7 (PTK7-MT) together with FLAG- or HA-tagged Ror2 deletions were expressed in MCF7 cells. A The respective constructs and their protein domains are depicted in the top panel. B Immunoprecipitation using anti-FLAG antibodies; the cell transfection scheme is indicated at the top. Co-precipitated PTK7 was detected using anti-myc antibodies. Immunoprecipitated PTK7 is shown in the top panel, immunoprecipitated Ror2 constructs in the middle panel and cell lysates in the bottom panel. Antibodies used for Western blotting and molecular weights are indicated at the right. C Immunoprecipitation using anti-myc antibodies; the cell transfection scheme is indicated at the top. Co-precipitated full-length Ror2 and sRor2 were detected using anti-HA antibodies (top panel). Immunoprecipitated PTK7 is shown in the middle panel and cell lysates in the bottom panel. Antibodies used for Western blotting and molecular weights are indicated at the right.

https://doi.org/10.1371/journal.pone.0145169.g002

PTK7 loss of function inhibits cell motility of explanted NC cells

As we detected co-localization of PTK7 and Ror2 in explanted NC cells, we used this system to further evaluate the biological relevance of the PTK7/Ror2 interaction. In Xenopus PTK7 has been shown to be required for NC migration [13], but its function on a cellular level remains elusive. Therefore, we used live-cell imaging to further analyze the migration behavior of explanted control and PTK7 morphant NC cells. Membrane-targeted GFP (mGFP) and cherry-labeled Histone2B (H2B-mcherry) were co-expressed to visualize cell shape and polarity. At the start of the experiment (0 hours), control NC cells formed a cell cluster where the leading-edge cells started to get polarized (Fig 3A, upper panel, S1 Movie). After one hour the leading cells left the main cell cluster and then dispersed rapidly during the 5 hour time-span of the experiment. In contrast, PTK7 MO injected NC cells adopted a more roundish shape and did not disperse as well as control cells (Fig 3 A, lower panel, S1 Movie). The dispersion of NC cells can be seen in the time series (Fig 3A), where–although explant size in the different experimental conditions is comparable at the beginning of the time-series–the area of cell spreading is significantly larger for the controls than the MO-injected explants after 5 hours of imaging. As it is difficult to determine the exact initial cell number and as the cell dissociation may not occur homogenously, we used cell tracking and Delaunay triangulation as more sophisticated methods to measure cell dispersion (Fig 3B and 3C). Delaunay triangulation determines the two closest cell neighbors and the area of the formed triangles is proportional to the cell dispersion. The time series as well as cell tracking and Delaunay triangulation all show that control cells dispersed efficiently during the 5 hours of imaging (Fig 3B). In contrast, in explants where PTK7 protein expression was knocked down, the NC cells had difficulties to detach from the main cluster and did not disperse as well as the control NC cells (Fig 3C). Higher magnification shows that PTK7 morphant cells do not form extensive protrusions like control cells, but adopt a more roundish shape. This shape change does not correlate with cell death as determined by Propidium iodine staining (S2 Fig). Some of these explants are still able to form protrusion, but others adopt a cell movement reminiscent of blebbing (Fig 3D, S2 Movie). Thus, PTK7 loss of function has severe effects on NC cell shape and motility.

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Fig 3. Loss of function of PTK7 affects NC cell shape and inhibits migration of explanted NC.

A Time series showing explanted NC cells injected with 7.5 ng control or PTK7 MO in combination with 50 pg mGFP RNA and 250 pg H2B-mcherry. Cranial NC explants were excised at stage 16–17 and explanted on a fibronectin matrix and incubated until they had stably adhered to the matrix. NC migration was monitored for 5 hours using spinning disk microscopy (10x objective NA 0.45). Images for representative explants injected either with control or PTK7 MO are shown at the start of the experiment (0 h) or after 1, 3 or 5 hours; scale bar = 50 μm. B Cell tracking and Delaunay triangulation for explants injected with 7.5 ng co MO. The upper panel shows a single frame of the spinning disk movie and the lower panel the Delaunay triangulation at the start of the experiment (0 h) or after 5 hours. Cells were tracked over the whole five-hour time interval using the H2B staining of single nuclei. These tracks are shown for single cells as differently colored lines in the images taken after 5 hours, scale bar = 50 μm. C Cell tracking and Delaunay triangulation for explants injected with 7.5 ng PTK7 MO. D Time series showing explants injected with 7.5 ng co MO (upper panel) or 7.5 ng PTK7 MO (lower panel) at a higher magnification. Injected NC cells were explanted at stage 17, cultured for 1.5 hours and imaged with a 63x objective (NA 1.4); scale bar = 20 μm. Images are shown at the start of the experiment (0 min) and after 45 and 90 minutes.

https://doi.org/10.1371/journal.pone.0145169.g003

Ror2 rescues the PTK7 loss of function NC migration defect and this requires the kinase domain of Ror2

As PTK7 and Ror2 are both non-canonical Wnt receptors, which share functions in Wnt signaling, we analyzed if Ror2 can rescue the PTK7 loss of function NC migration defect. To this end NC cells injected either with co MO or PTK7 MO alone or in combination with Ror2 were explanted and their migration was analyzed by time-lapse imaging. While the majority of PTK7 MO injected NC cells showed migration defects, NC cells, which were co-injected with the PTK7 MO and Ror2 showed normal NC migration behavior (Fig 4, S3 Movie). Defects in protrusion formation as well as cell motility were rescued by co-expression of Ror2 (Fig 4A and 4B, S3 Movie) indicating that Ror2 expression can substitute for PTK7 in NC cells.

Similar results were obtained in in vivo experiments where NC migration was analyzed at tadpole stages using twist in situ hybridization. Embryos injected with co MO were unaffected, while the majority of embryos injected with the PTK7 MO showed severe NC migration defects (Fig 5A, 5B and 5F). Co-injection of Ror2 RNA with the PTK7 MO significantly decreased the percentage of embryos showing NC migration defects (Fig 3C and 3F). In contrast co-injection of a deletion mutant of Ror2 lacking major parts of the intracellular domain, Ror2Δ469 (Fig 2A and 2F) did not show a significant rescue defect (Fig 3D and 3F). Interestingly, a kinase dead mutant of Ror2 (Ror2-3I), where three lysines at position 504 (in the putative ATP-binding motif), 507 and 509 were all replaced with isoleucine [57] to abolish the catalytic activity, was not able to rescue the PTK7 morphant NC migration defect. Thus, these results suggest that Ror2 can functionally replace PTK7 and that the kinase function of Ror2 is required for this activity.

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Fig 4. Ror2 rescues the PTK7 loss of function phenotype in explanted NC cells.

A NC explants injected with 7.5 ng MO in combination with 50 pg mGFP RNA, 250 pg H2B-mcherry and 150 pg Ror2 RNA. Time-lapse images (upper panel) and Delaunay triangulations (lower panel) at the start of the experiment (0 h) and after 4 or 8 hours are shown for the different conditions. B Graph summarizing percentage of migration defects of 3 independent experiments (total of 39 explants). Standard error of the means are shown. Asterisks indicates a p-value in a Student’s t-test < 0.05. Scale bars = 200 μm.

https://doi.org/10.1371/journal.pone.0145169.g004

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Fig 5. The kinase domain of Ror2 is required to rescue the NC migration defect in PTK7 morphant embryos.

Xenopus embryos were injected with different constructs in combination with 100 pg LacZ RNA as a lineage tracer and analyzed by whole-mount in situ hybridization using a twist antisense RNA probe. A Embryo injected with 10 ng control MO and 100 pg GFP RNA shows normal NC migration. B Embryo injected with 10 ng PTK7 MO and 100 pg GFP RNA shows inhibition of NC migration on the injected side, while NC migration is normal on the uninjected side. C Co-injection of 10 ng PTK7 MO together with 100 pg Ror2 RNA rescues the NC migration defect. D Embryo injected with 10 ng PTK7 MO and 100 pg of Ror2Δ469 RNA. The embryo shows a NC migration defect on the injected side. E Embryo injected with PTK7 MO and a kinase dead mutant of Ror2 (Ror2-3I) showing a NC migration defect on the injected side. F Graph summarizing the percentage of NC migration defects of a minimum of 5 independent experiments for each experimental condition. Asterisks indicates a p-value in a Student’s t-test < 0.001. Scale bar = 500 μm.

https://doi.org/10.1371/journal.pone.0145169.g005

Discussion

The PTK7 receptor functions in various processes ranging from embryonic morphogenesis to wound repair and its distinct functions are likely regulated by receptor context. PTK7 has been shown to interact with Frizzled7 and LRP6 ([13,35,38], however, an interaction with bona fide non-canonical Wnt receptors has so far not been analyzed. Concerning the PTK7 receptor complex our data suggest that PTK7 may interact with the non-canonical Ror2 Wnt-receptor. Like PTK7, Ror2 is expressed in migrating NC cells [57,60] and a role in NC development has been suggested [69]. Further, using fluorescently labeled PTK7 and Ror2 we detect a co-localization of both proteins in NC cells. Co-immunoprecipitation experiments show an interaction of Ror2 with PTK7 and suggest the extracellular domains as possible sites of interaction. More specifically, we observed that a Ror2 mutant lacking most of the extracellular part except the Ig-like domain was sufficient for binding to PTK7. Both, PTK7 and Ror2 possess Ig-like domains, which mediate a large variety of protein-protein interactions (for review see [70]). However, we can currently not rule out that there may be additional interaction sites. As co-immunoprecipitation experiments were performed in cell lysates it remains unclear if this interaction is direct or for example mediated by Wnt ligands or Frizzled co-receptors. Since interaction of both PTK7 and Ror2 with Frizzled receptors has been shown [13,36,50,55], members of the Frizzled receptor family may also contribute to the PTK7/Ror2 interaction. In addition, PTK7 [35,36] and Ror2 interact with different members of the Wnt protein family, although in the case of Ror2 only non-canonical Wnts such as Wnt-5a were found to activate downstream signaling [49,50,57,71]. Thus, although the exact molecular composition of a PTK7/Ror2 receptor complex remains yet to be analyzed, the formation and composition may also affect the selective binding to members of the Wnt family and thereby modulate intracellular signaling events.

The downstream signaling events of the PTK7/Ror2 complex are currently unclear, but may be mediated by an activation of c-Jun N-terminal kinase (JNK). Previously, we have shown that PTK7 overexpression in Xenopus ectodermal explants leads to a nuclear localization of phosphorylated JNK [13] and this is not observed if a kinase deletion mutant of PTK7 (∆kPTK7) is overexpressed (data not shown). Ror2 has been shown to have a similar function [44,46,53]. Therefore, possibly these molecules collaborate to recruit Dishevelled and to activate JNK via the PCP pathway [72] and thereby enable the migration of NC cells [8,9,17,18,25]. Supporting this hypothesis, Ror2 overexpression can rescue the PTK7 loss of function NC migration defect possibly by compensating for PTK7. Consistently, this rescue effect requires the kinase domain of Ror2, which is necessary for JNK activation [44]. In the context of mesodermal convergent extension it was previously shown that Ror2 activates a signaling cascade including PI3K, the small GTPase cdc42, MKK7, JNK and c-jun and ATF2 transcription factors [44]. For PTK7 the mechanism of JNK activation remains unclear, but may involve the adaptor protein RACK1, which is required for PTK7-mediated Dishevelled recruitment [30]. As RACK1 plays a role in PKC-mediated JNK phosphorylation [73], PTK7 may activate JNK via RACK1 or Dishevelled itself [72]. JNK is involved in a multitude of processes and can activate the transcription factors jun, fos and ATF2 [74]. Like Ror2, PTK7 is also able to activate ATF2-mediated transcription as shown by activation of an ATF2 luciferase reporter in Xenopus lysates [35,75]. Thus, one of the common signaling outcomes of PTK7 and Ror2 may be the transcription of ATF2-dependent target genes, which has previously also been acknowledged as a readout for a bona fide activator of non-canonical Wnt signaling [75].

In addition to activation of JNK, PTK7 and Ror2 share a function in the inhibition of canonical Wnt signaling [34,35,45,53], which may contribute to their role in NC development. The molecular mechanisms are currently unclear. Ror2 may inhibit canonical Wnt signaling by regulating the stability of ß-catenin [49] or Tcf/Lef-dependent transcription [45,53]. Furthermore, it has been suggested that formation of a non-canonical Wnt5a/Ror2/Frizzled complex could compete for available Frizzled receptors thereby inhibiting the formation of a canonical Wnt3a/LRP/Frizzled complex [47] and subsequently canonical Wnt signaling. PTK7 likely inhibits canonical Wnt signaling by a similar mechanism. As PTK7 can form a complex with canonical Wnt ligands and Frizzled7 [35], it could also sequester Frizzled7 in a non-canonical Wnt signaling complex thereby preventing its interaction with canonical co-receptors and Wnt ligands. Indeed, it has been shown that Frizzled7 functions in canonical and non-canonical Wnt signaling depending on Wnt- and likely also co-receptor-context [76]. Independent of the mechanism by which PTK7 or Ror2 inhibit canonical Wnt signaling, this activity could explain their function in NC migration. Previously, it has been shown that ectopic activation of canonical Wnt signaling inhibits NC migration. Explanted NC cells treated with LiCl, an inhibitor of GSK3ß, or exogenous Wnt1 show an inhibition of NC migration [77]. Thus, loss of function of PTK7 could enhance canonical Wnt signaling thereby inhibiting NC migration. As Ror2 can also inhibit canonical Wnt signaling, this may account for its ability to substitute for PTK7 in NC cells. Currently it remains unclear by which mechanism a PTK7/Ror2 complex affects NC migration. We have shown that the tyrosine kinase domain of Ror2 is required to rescue PTK7 loss of function. However, as the tyrosine kinase activity of Ror2 is required for both activation of JNK and inhibition of canonical Wnt signaling [44,78], Ror2 is capable to compensate for both putative functions of PTK7. Therefore, it remains to be seen which one of these or if possibly even a combination of both is required. In summary, PTK7 and Ror2 share signaling functions that may be enhanced by the combination of both molecules and that may allow for example Ror2 to compensate for PTK7.

A possible interaction of PTK7 with Ror2 is likely not limited to NC cells, as PTK7 and Ror2 have also been implicated in tumor development and progression. PTK7 and Ror2 expression is frequently deregulated in a variety of cancers [79,80,81,82,83,84,85]. Several studies indicate an association of Ror2, in some cases downstream of Wnt5a, with tumor invasiveness and metastasis [82,84,86,87,88]. However, it has been shown that epigenetic silencing of Ror2 in colon cancer promotes cell proliferation and tumor growth [89], suggesting that depending on the cellular context Ror2 could also act as a tumor-suppressor. Similarly, contradictory results are also seen for the function of PTK7. PTK7 is expressed in acute myeloid leukemia, where it promotes cell migration and leads to a poor clinical outcome [90]. Additional studies found migration and invasion promoting functions of PTK7 in lung cancer, intrahepatic cholangiocarcinoma, glioma and prostate cancer [42,91,92,93]. In contrast, another publication shows that PTK7 is a target of membrane type-1 matrix metalloproteinase and that PTK7 expression inhibits cell invasion [94]. Thus, the function of PTK7 and Ror2 may depend on tumor context and ultimately on receptor context.

Supporting Information

S1 Fig. PTK7 and Ror2 co-precipitate each other but not EGFR or TGFß1R.

A Full-length myc-tagged PTK7 (PTK7-MT) was co-expressed with Ror2-EGFP, EGFR-EGFP [95] or TGFß1R-EGFP (kind gift of A. Menke, Molecular Oncology of Solid Tumors, Giessen, Germany) as indicated in MCF7 cells. Cell lysates were precipitated using anti-myc antibodies (IP α-MT, upper panel). Precipitates are shown in the upper panels, cell lysates in the lower panel. Antibodies used for Western blotting and molecular weights are indicated at the right. B Full-length HA-tagged Ror2 was co-expressed with PTK7-MT, EGFR-EGFP or TGFß1R-GFP as indicated in MCF7 cells and cell lysates were precipitated using anti-HA antibodies (IP α-HA, upper panel). Precipitates are shown in the three upper panels and lysates in the two lower panels. Antibodies used for Western blotting and molecular weights are indicated at the right; * marks unspecific bands, ** Ror2 signal remaining from previous anti-HA staining, which was only partially removed by blot stripping.

https://doi.org/10.1371/journal.pone.0145169.s001

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S2 Fig. Propidium Iodine (PI) staining to determine the viability of PTK7 loss of function NC cells.

NC explants injected with 7.5 ng control MO or PTK7 MO in combination with 50 pg mGFP were treated with PI (10 μg/ml) to test for the viability of the explanted NC cells. Cell membrane integrity prevents PI staining of viable cells, while it can stain nucleic acids of apoptotic cells (red). In PTK7 morphant cells few PI-positive cells appear after 3 hours compared to 6 hours in controls (one example each is marked by a red arrow). Round-shaped PTK7 morphant NC cells appear early in the experiment and keep moving/blebbing for up to a couple of hours (white arrows, numbers indicate specific cells during the course of the experiment). Thus, the roundish cell shape is not necessarily an indication of cell death. Dashed squares show higher magnifications of specific cells. Scale bar = 50 μm.

https://doi.org/10.1371/journal.pone.0145169.s002

(TIF)

S1 Movie. Time-lapse movie showing the migration of NC cells injected with control MO (left) in comparison to an explant injected with PTK7 MO.

Images were taken using a Spinning Disk microscope with a 10x plan apochromat objective NA (0.45).

https://doi.org/10.1371/journal.pone.0145169.s003

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S2 Movie. Higher magnification using a 63x plan apochromat objective NA 1.4) of control MO (left) and PTK7 MO injected (right) NC explants.

https://doi.org/10.1371/journal.pone.0145169.s004

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S3 Movie. Time-lapse movie showing that Ror2 expression rescues the PTK7 morphant phenotype.

Explants injected with control MO (upper panel, left), control MO and Ror2 RNA (upper panel, right), PTK7 MO (lower panel, left) or PTK7 MO and Ror2 RNA (loer panel, right) are shown over a time interval of 8 hours and 20 minutes.

https://doi.org/10.1371/journal.pone.0145169.s005

(AVI)

Acknowledgments

We would like to especially thank Alexandra Schambony for constructs and helpful discussions and comments on the manuscript. We thank Iryna Shnitsar for cloning of the PTK7-RFP and Jubin Kashef and Andre Mencke for supplying plasmids. Further, we thank Ingrid Bohl-Maser and Christiane Rohrbach for technical assistance and Melanie Bernhardt for taking excellent care of our Xenopus colony.

Author Contributions

Conceived and designed the experiments: AB MP AG HB. Performed the experiments: MP AG HB ER. Analyzed the data: AB MP AG HB ER. Contributed reagents/materials/analysis tools: AB. Wrote the paper: AB MP AG.

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