Figures
There are errors in the lower panels of Fig 4A and Fig 4D of the published article. Please see the correct Fig 4 here. The original images for all of the lower panels in Fig 4 are included as Supporting Information files to this correction. The authors apologize for the errors.
E. coli strains MC4100relA+ (WT), MC4100relA+ΔmazEF, and MC4100relA+ ΔdinJ-yafQ were grown at 37°C for 24 hr in 96 well polystyrene plates in LB (A,B,C) or M9 (D,E,F) media. Attached cells were washed and planktonic cells were removed as described (Materials and methods). Dead cells stained with PI (red) and living cells retained staining with SYTO 9 (green). (A,D) Biofilms were photographed by CLSM with a using ×2.5, ×10 magnifications. The entire well was photographed from above. The image is a representative image from three independent experiments; each experiment was carried out in quadruplicate. Additionally, cells stained with SYTO 9 or PI were quantified as described in “materials and methods”. (B,E) Percentage of dead cells from the total population in the biofilm. (C,F) Absolute number of dead cells. Data were obtained from two independent experiments, performed quadruplicate.
Reference
Citation: Kolodkin-Gal I, Verdiger R, Shlosberg-Fedida A, Engelberg-Kulka H (2015) Correction: A Differential Effect of E. coli Toxin-Antitoxin Systems on Cell Death in Liquid Media and Biofilm Formation. PLoS ONE 10(10): e0140184. https://doi.org/10.1371/journal.pone.0140184
Published: October 2, 2015
Copyright: © 2015 Kolodkin-Gal et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited