The development of social behavior is strongly influenced by the serotonin system. Serotonin 2c receptor (5-HT2cR) is particularly interesting in this context considering that pharmacological modulation of 5-HT2cR activity alters social interaction in adult rodents. However, the role of 5-HT2cR in the development of social behavior is unexplored. Here we address this using Htr2c knockout mice, which lack 5-HT2cR. We found that these animals exhibit social behavior deficits as adults but not as juveniles. Moreover, we found that the age of onset of these deficits displays similar timing as the onset of susceptibility to spontaneous death and audiogenic-seizures, consistent with the hypothesis that imbalanced excitation and inhibition (E/I) may contribute to social behavioral deficits. Given that autism spectrum disorder (ASD) features social behavioral deficits and is often co-morbid with epilepsy, and given that 5-HT2cR physically interacts with Pten, we tested whether a second site mutation in the ASD risk gene Pten can modify these phenotypes. The age of spontaneous death is accelerated in mice double mutant for Pten and Htr2c relative to single mutants. We hypothesized that pharmacological antagonism of 5-HT2cR activity in adult animals, which does not cause seizures, might modify social behavioral deficits in Pten haploinsufficient mice. SB 242084, a 5-HT2cR selective antagonist, can reverse the social behavior deficits observed in Pten haploinsufficient mice. Together, these results elucidate a role of 5-HT2cR in the modulation of social behavior and seizure susceptibility in the context of normal development and Pten haploinsufficiency.
Citation: Séjourné J, Llaneza D, Kuti OJ, Page DT (2015) Social Behavioral Deficits Coincide with the Onset of Seizure Susceptibility in Mice Lacking Serotonin Receptor 2c. PLoS ONE 10(8): e0136494. https://doi.org/10.1371/journal.pone.0136494
Editor: Makoto Sato, Osaka University Graduate School of Medicine, JAPAN
Received: January 17, 2015; Accepted: July 15, 2015; Published: August 26, 2015
Copyright: © 2015 Séjourné et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Data Availability: All relevant data are within the paper.
Funding: This work was supported by startup funds from The Scripps Research Institute (D. T. P.), gift funds from Ms. Nancy Lurie Marks (D. T. P.), and National Institute of Health (NIH) grant R01MH105610 (D. T. P.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Serotonin (5-HT) is a key neurotransmitter that appeared early in evolution  and influences a variety of social processes  across species, from humans  to primates , rodents  and flies . The serotonin receptor 5-Ht2cR, encoded by the 5Htr2c gene, is a G protein-coupled receptor (GPCR) that is coupled to Gq/G11 and modulates cellular excitability . Pharmacological studies in animal models have demonstrated roles for 5-Ht2cR in communication [8, 9] and social interaction. For example, activation of 5-Ht2cR by mCPP [10, 11], a non-selective agonist, or by SSRI (selective serotonin reuptake inhibitor, indirect agonist via inhibition of the serotonin transporter SLC6A4)  reduces social interaction in rodents. Conversely, administration of the selective 5-Ht2cR antagonist SB 242084 in rats increases social interaction  and rescues social deficits caused by stress  or mCPP [10, 11].
Dysregulated activity of 5-HT2cR has been implicated in autism spectrum disorder (ASD), which features deficits in social interaction and communication (DSM-V, American Psychiatric Publishing, 2013). For example, hyperactivity of 5-HT2cR has been reported in two mouse models of ASD risk factors: in mice with chromosome 15q11-13 duplication due to overexpression of the 5-HT2cR-editing snoRNA MBII52 , and in mice with a loss of function mutation for methyl-CpG binding protein 1 . In addition, there is evidence of a physical interaction between 5-HT2cR and Pten [16, 17], a negative regulator of the PI3-kinase pathway  and a risk factor for ASD [19–22]. However, it is not known how chronic alteration in 5-HT2cR activity during development affects social behavior. Thus, we examined the social behavior of juvenile and adult Htr2c knockout mice, which lack 5-HT2cR.
Interestingly, null mutant mice lacking 5-HT2cR are extremely susceptible to audiogenic seizures [23, 24], suggesting an underlying elevation of the ratio of cellular excitation to inhibition (E/I balance) in these mice. It has been speculated that social and cognitive deficits might arise from a modification in this E/I balance, for example, through increased activity in excitatory neurons or reduced inhibitory neuron activity [25–29]. Supporting this idea, elevation of the E/I balance in the prefrontal cortex in mice elicits a profound impairment of social behavior . To examine this relationship, we also investigated the onset of audiogenic seizure susceptibility in Htr2c knockout mice.
Material and Methods
Strains used were B6.129-Htr2ctm1Jke  (from The Jackson Laboratory) and B6.129-Htr2ctm1Jul  (from The Jackson Laboratory). Both lines were crossed to a C57BL/6J background for at least 10 generations to reach congenicity. Mice of the B6.129-Ptentm1Rps line  were obtained from the repository at the National Cancer Institute at Frederick, where they were already backcrossed onto a congenic C57BL/6J background by the Donating Investigator. The line has been maintained by backcrossing to C57BL/6J mice for more than 10 generations. For behavioral experiments female Htr2ctm1Jke/+ mice were crossed with C57BL/6J males, thus producing Htr2ctm1Jke/Y (referred to as Htr2c-/Y) and Htr2c+/Y (referred to as wild-type) male offspring. For the analysis of spontaneous death, female Htr2ctm1Jul/+ or Htr2ctm1Jke/+ mice were crossed with Ptentm1Rps/+ males, resulting in Htr2+/Y, Ptentm1Rps/+ (referred to as Pten+/-) and Htr2Jke/Y; Ptentm1Rps/+ (referred to as Htr2c-/Y; Pten+/-) male offspring. No distinction is made between the Htr2ctm1Jul and Htr2ctm1Jke lines in the analysis of spontaneous death as they are phenotypically equivalent. Due to the localization of the Htr2c gene on the X chromosome and to random X chromosome inactivation, no female Htr2c+/- mice were used in this study to avoid complications arising from mosaicism.
Behavioral testing occurred between postnatal days 21 to 26 (P21–26) for juveniles and P85–90 for adults. All animals were housed in mixed-genotype groups of 2–5 mice per cage, with no differences in housing between genotypes. Food and water were provided ad libitum and animals were kept on a reversed 12-h light/dark cycle. All behavioral testing was performed during the dark (active) phase of the light cycle. Experiments were performed in accordance with National Institute of Health and Association for Assessment and Accreditation of Laboratory Animal Care guidelines and approved by The Scripps Research Institute’s Institutional Animal Care and Use Committee.
Three-chamber social approach and social novelty
Juvenile and adult mice were tested as previously described [33, 34] under white-light conditions. Briefly, test mice were each placed into the center of a black acrylic center arena (60 x 30 x 30 cm) that was divided into three equal compartments (each 20 x 30 x 30 cm). Mice were habituated to the empty arena for 5 min on each of the two days prior to testing. The test day consisted of three phases: 5 min acclimation to the empty arena, 10 min sociability testing [choice between two acrylic tubes (20 cm tall, 10 cm in diameter, with 16 ¼” diameter holes in the bottom half of the cylinder), one containing a novel, same-sex conspecific (location counterbalanced across mice), the other being empty], and 10 min social novelty testing (novel, same-sex conspecific placed in the previously empty tube). Tubes and chambers were cleaned with quatricide and paper towel-dried between mice. Ethovision (Noldus Information Technology, Wageningen, The Netherlands) was used to score the time spent in each chamber, as well as the velocity and distance traveled, for each mouse. Different cohorts of mice were used for juvenile and adult experiments.
Audiogenic seizure (AGS) testing
Juvenile (P25) and adult animals (P90) were moved to the testing area and left undisturbed for 1–3h prior to testing. AGS testing was performed using a Phenotyper box (29.2 x 29 x 30.5 cm, Pten-T10/N, Noldus Information Technology, Wageningen, The Netherlands) equipped with a speaker and clear walls, each inside a noise-attenuating box with fans on. Behavior was monitored via a CCD camera mounted on the ceiling of the box and recorded by Ethovision. After a 30 s period of acclimation to the chamber, a 108-dB white noise stimulus was maintained for 60 s or until overt seizure had occurred. Mice that exhibited no sign of seizure during the stimulus were then monitored for 30 min. The motor response to audiogenic stimulus was classified as described previously : no response (NR), wild running (WR), clonic seizure (CS), tonic seizure (TS), respiratory arrest/death (RA).
A total of 68 mice were used in the lifespan study with 20 wild-type mice, 21 Pten+/-, 15 Htr2c-/Y; Pten+/- and 12 Htr2c-/Y; Pten+/-. Mice were maintained in standard conditions with 5 mice per cage and were permitted to live out their lives until death due to natural causes. The mice used in the lifespan study were not disturbed except to check on the mice twice each day and were euthanized if any sign of distress was observed following AAALAC recommendations.
20 minutes prior to testing, mice tested for social approach with 5-HT2cR antagonist were given an intraperitoneal injection of SB 242084 (Sigma-Aldrich) diluted to 0.3mg/ml in a 10% (2-hydroxypropyl)-β-cyclodextrin (Sigma-Aldrich) in sterile 25mM citric acid vehicle, or equivalent volume vehicle. For all injections, care was taken to handle animals gently to minimize stress.
Independent-sample t-tests were used to assess the effects of genotype (wild-type, Htr2c-/Y) on behavior, and paired-sample t-tests were used to analyze chamber preferences for the three-chamber social approach (% time in mouse chamber vs. % time in empty tube chamber) and novelty test (% time in novel mouse chamber vs. % time in familiar mouse chamber) for each genotype. Additionally, approach-avoidance scores [time in chamber with a social stimulus minus time in chamber with the empty tube; [34, 36]] were calculated, and genotypes were compared using independent-sample t-tests (wild-type, Htr2c-/Y) or two-way analyses of variance (drug: vehicle, 0.3mg/kg SB 242084; genotype: wild-type, Htr2c-/Y). Kaplan-Meier survival analysis was used with Log-Rank test followed by pairwise comparison (Holm-Sidak) to analyze the data. For the pharmacogenetic study, planned comparisons between genotypes for each drug (vehicle: wild-type vs. Htr2c-/Y, SB 242084: wild-type vs. Htr2c-/Y) and between drug treatment for each genotype (wild-type: vehicle vs. SB 242084, Htr2c-/Y: vehicle vs. SB 242084), were performed using independent-sample t-tests. In all cases, normality was assessed using Levene’s test. All statistics were performed using PASW 18 (IBM Corporation, Armonk, NY, USA), with significance set at p<0.05. All graphs represent mean +/- SEM.
We assessed the development of social behavior in Htr2c knockout mice using the three-chamber social approach and social novelty assay [33, 34] as juveniles (P21–26) or adults (P85–90). Both wild-type and Htr2c-/Y juvenile males spent significantly more time in the chamber with the novel social stimulus versus the chamber with the object control during the social approach assay (t(8) = 2.39, p<0.05 and t(8) = 2.35, p<0.05 respectively, Fig 1A left) and in the chamber with the novel social stimulus versus the chamber with the familiar social stimulus during the social novelty assay (t(8) = 2.34, p<0.05 and t(8) = 2.64, p<0.05 respectively, Fig 1A right). Correspondingly, we did not find a significant difference between wild-type and Htr2c-/Y juvenile mice for the approach-avoidance score (t(16) = 0.30, p = 0.77, Fig 1B). In order to determine the proportion of mice that show a strong preference for the mouse chamber, we designed a preference index, calculated as: (number of mice where the time in chamber 1 (containing stimulus mouse in cage) was ≥ 10% of the time spent in chamber 3 (containing empty cage))–(number of mice where the time in chamber 1 was < 10% of the time spent in chamber 3) / (total number of mice). Both wild-type mice (55.5%) and Htr2c-/Y mice (33.3%) presented a high positive preference index, showing that more than half of the mice had a strong preference for the mouse chamber (Fig 1C). Additionally, there was no significant difference in velocity or distance traveled in the social approach phase (t(16) = 0.73, p = 0.47 and t(16) = 0.62, p = 0.55 respectively, Fig 1D). These results suggest that Htr2c deletion does not impact social behavior of juvenile mice. Next, we wanted to test if constitutive deletion of Htr2c affects social behavior in adult mice.
(A) Time spent in each chamber. (B) Approach-avoidance scores. (C) Preference index: [(number of mice where the time in the mouse chamber was ≥ 10% than the time spent in the tube chamber)–(number of mice where the time in the mouse chamber was < 10% than the time spent in the tube chamber)]/(total number of mice). (D) Velocity and distance traveled. n = 9 per genotype. *: p<0.05, NS: non-significant difference with paired samples t-test (A) and independent-samples t-test (B and D).
Wild-type adult males spent significantly more time with the social stimulus during social approach (t(15) = 7.90, p<0.001, Fig 2A left), and with the novel social stimulus during social novelty (t(15) = 3.28, p<0.01, Fig 2A right), while Htr2c-/Y adult males showed no significant differences in chamber time during these assays (t(16) = 1.19, p = 0.25 and t(16) = 1.31, p = 0.21 respectively, Fig 2A). Analyzing these data using a social approach-avoidance score, we found that the time spent interacting with a stimulus mouse was significantly less in Htr2c-/Y than in wild-type mice (t(22.3) = 2.47, p<0.05, Fig 2B). The individual approach-avoidance score of Htr2c-/Y mice showed a bimodal distribution that was reflected by a higher variation in this score than in wild-type mice (Levene’s test for equality of variance: F = 14.91, p<0.01, Fig 2C). Using the preference index, we found a high preference index for wild-type mice (87.5%) while Htr2c-/Y mice showed a negative score (-12.5%; Fig 2D) confirming that less than half of these mice showed a preference for the mouse chamber. Moreover, we found that velocity and distance traveled in the social approach assay were significantly increased in Htr2c-/Y compared to wild-type mice (t(30) = 2.13, p<0.05 and t(30) = 2.46, p<0.05 respectively, Fig 2E) confirming previous studies [31, 37]. Thus, constitutive deletion of Htr2c decreased social interaction and increased locomotor activity in adult mice.
(A) Time spent in each chamber. (B) Approach-avoidance scores. (C) Individual approach-avoidance scores of adult males. (D) Preference index: [(number of mice where the time in the mouse chamber was ≥ 10% than the time spent in the tube chamber)–(number of mice where the time in the mouse chamber was < 10% than the time spent in the tube chamber)]/(total number of mice). (E) Velocity and distance traveled. n = 16 per genotype. *: p<0.05, **: p<0.01, ***: p<0.001 with paired samples t-test (A) and independent-samples t-test (B and E).
Adult Htr2c knockout mice feature spontaneous death and audiogenic seizures (AGS), which has been interpreted as reflecting a role for 5-HT2cR in tonic inhibition of excitability in neuronal networks [24, 38]. E/I imbalance within neural networks has been hypothesized as underlying social behavioral deficits in ASD and a variety of other neuropsychiatric disorders [25–28]. In light of this hypothesis, we find it interesting that adult, but not juvenile, Htr2c knockout mice display deficits in sociability. To further explore the relationship between social behavioral deficits and seizure susceptibility, we tested for AGS at P25 and P90, after social behavioral testing was complete in juvenile and adult cohorts of mice, respectively. No Htr2c-/Y mice displayed seizures at P25, confirming previous findings that juvenile Htr2c-/Y mice are not susceptible to AGSs . At P90, we found that all Htr2c-/Y mice, but no wild-type mice, exhibited tonic-clonic seizures manifested by a brief forelimb and hindlimb flexion followed by a rigid and prolonged hindlimb extension (Fig 3). The latency to the tonic-clonic phase was extremely short, ranging from 2 to 6 seconds after the start of the white noise (average: 4.12 s). This confirms that the timing of social behavior deficits coincides with the onset of seizure susceptibility in Htr2c mice. It remains to be determined whether a causal relationship exists between these two phenotypes.
(A) Htr2c-/Y adult mice (P90) show a severe response to audiogenic stimulus (RA: n = 16) while Htr2c-/Y juvenile mice (P25) or wild-type adult and juvenile did not exhibit any response (NR: n = 4, 16, 4 respectively). NR, no reponse; WR, wild running; CS, clonic seizure; TS, tonic seizure; RA, respiratory arrest/death. (B) The seizure severity score indicated a fully penetrant phenotype in adult Htr2c-/Y mice.
Pten is a significant risk factor for ASD in humans [19–22] and various mouse models of Pten deletion show a deficit in social behavior [33, 34, 39, 40]. It has been shown previously that haploinsufficiency for the Slc6a4 gene, encoding the serotonin transporter, can exacerbate the social behavior deficits observed in Pten+/- mice . Moreover, Pten and 5-HT2cR receptor interact biochemically in dopaminergic neurons of the ventral tegmental area , making mutations in Pten a strong candidate for modifying the phenotypes observed in Htr2c-/Y mice. As Htr2c deletion did not affect the preference of the juvenile mice in the 3-chamber assay (Fig 1), we aimed to investigate the social behavior of Htr2c-/Y; Pten+/- adults. However, we found that only 20% of Htr2c-/Y; Pten+/- mice survived to P90 (Fig 4). This made adult social behavioral testing impractical in these animals. Wild-type (n = 20) and Pten+/- mice (n = 21) did not exhibit any spontaneous death within the timeframe examined (up to P180). An autopsy of mice that died spontaneously did not reveal any noticeable health problems such as hemorrhage, infarction, ischemia, hamartomas or tumors. Thus, as previously observed for Htr2c-/Y mice , we conjecture that the early lethality of Htr2c-/Y; Pten+/- mice may be attributed to an increase in spontaneous epileptic seizures. Using a Kaplan-Meier analysis, we found that there was a significant effect of genotype on survival (Log-Rank test: F(2) = 36, p<0.001). Pairwise comparisons revealed a significant difference on the survival of Pten+/- mice vs. Htr2c-/Y (p<0.001) and Htr2c-/Y; Pten+/- mice (p<0.001) and also between Htr2c-/Y and Htr2c-/Y; Pten+/- mice (p<0.05). We interpret these results as consistent with mutations in Pten and Htr2c interacting to influence spontaneous death, possibly via an exacerbation of the epileptic mechanism present in Htr2c knockout mice. However, because of the accelerated age of death, we were prevented from testing social behavior in these compound mutant animals.
Time course of spontaneous death in Htr2c-/Y (n = 15), Pten+/- (n = 21) and Htr2c-/Y; Pten+/- (n = 12) mice.
As an alternative approach to explore whether Pten and 5-HT2cR interact to influence social behavior, we used SB 242084, a selective antagonist of 5-HT2cR that has been shown to increase social interaction behavior  and rescue social investigation deficits associated with stress  without increasing seizure susceptibility  in rodents. We used adult female Pten haploinsufficient and wild-type female mice as social approach deficits in female Pten+/- mice are well described [33, 34]. We administered 5-HT2cR antagonist SB 242084 systemically at 0.3 mg/kg, a dose that has been shown to significantly increase social interactions in rats [11, 13], and tested social approach behavior using a three-chamber social approach apparatus. Vehicle-treated wild-type animals spent significantly more time in the chamber containing the social stimulus mouse than the chamber containing an empty tube, while vehicle-treated Pten+/- mice did not display this preference (t(15) = 5.23, p<0.001 and t(16) = 0.40, p = 0.70, Fig 5A), indicating a deficit in sociability consistent with previous findings in untreated Pten+/- animals [33, 34]. Wild-type and Pten+/- mice treated with SB 242084 both displayed a significant preference for the chamber containing the social stimulus mouse (t(14) = 4.32, p<0.001 and t(15) = 4.38, p<0.001, Fig 5A). Analyzing these data using a social approach-avoidance score, we found a significant effect of genotype or SB 242084 treatment on the time spent to interacting with the stimulus mouse (Two-way ANOVA: Effect of genotype: F (1, 63) = 5.32, p<0.05. Effect of SB 242084 treatment: F (1, 63) = 6.32, p<0.05. Interaction between genotype and SB 242084 treatment: F (1, 63) = 1.97, p = 0.17 (n.s.), Fig 5B). Planned comparisons revealed a significant difference between Pten+/- mice treated with vehicle only and Pten+/- mice treated with SB 242084 (t(31) = 2.73, p<0.01) and between wild-type mice treated with vehicle and Pten+/- mice treated with vehicle (t(31) = 2.82, p<0.01), but no significant difference between wild-type treated with vehicle and wild-type treated with SB 242084 (t(29) = 0.76, p = 0.45) and between wild-type mice treated with SB 242084 and Pten+/- mice treated with SB 242084 (t(29) = 0.56, p = 0.58). Additionally, we found that, while Pten+/- mice treated with vehicle presented a very low preference index (5%), wild-type mice treated with vehicle or SB 242084 and Pten+/- mice treated with SB 242084 all presented a high positive preference index (50, 60 and 37.5% respectively). There was no spontaneous death or other evidence of seizures in Pten+/- mice treated with SB 242084. These results show that SB 242084 treatment suppresses the social behavior deficits exhibited by Pten+/- mice.
Results of three-chamber social approach after administration of SB 242084 or vehicle to wild-type and Pten+/- adult female mice. (A) Time spent in each chamber. For vehicle treated groups, n = 16 wild-type and 17 Pten+/-; for SB 242084-treated groups, n = 15 wild-type and 16 Pten+/-. *** p < 0.001 using paired-samples t-test (B) Approach-avoidance score. ** p < 0.01 using planned comparisons. (C) Preference index.
We report here that adult Htr2c null mutant mice present social behavior deficits and that these deficits are restricted to adult mice and are not present in juvenile mice. It has been hypothesized that dysregulated E/I balance may contribute to social behavioral deficits in neuropsychiatric disorders such as ASD [25–29] and consistent with this idea, it has been shown that elevation of cellular E/I balance within the mouse medial prefrontal cortex elicits an impairment of social behavior . Evidence supports that E/I imbalance is also responsible for seizure susceptibility and spontaneous death in Htr2c mutant mice [24, 41, 42]. Interestingly, similar to social behavior deficits, Htr2c null mutant mice present a susceptibility to AGS only in adulthood [23, 24, 31] (and Fig 3). Given this, together with the observation that pharmacological antagonism of 5-HT2cR does not increase susceptibility to seizures and can increase social investigation in rodents [10, 11, 13], it is reasonable to speculate that E/I imbalance in adult Htr2c knockout mice is responsible for both social behavior deficits and susceptibility to spontaneous and audiogenic seizures. Similarly, E/I imbalance might be responsible for both social behavioral deficits and epilepsy in some ASD patients, as it has been shown that 30% of ASD patients can present a co-morbidity for epilepsy . Alternatively, it is possible that mutations in Htr2c lead to an early dysregulation of growth and connectivity that results in abnormal neural circuitry going into the critical period for social learning, with social deficits not manifesting until after the critical period closes. It has been shown, for example, that social isolation that occurs in a critical period of 2 weeks after weaning alters prefrontal cortex function and myelination and these phenotypes are not reversible after reintroduction into a social environment . For this hypothesis, dopaminergic neurons are a strong candidate cell type since they are important for social behavior [33, 45–49] and express 5-Ht2cR . Future experiments using timed conditional deletions of Htr2c will help answer this question.
We also find that a second site mutation in Pten accelerates the spontaneous death rate observed in Htr2c-/Y mice [23, 24]. Consistent with previous reports in Htr2c-/Y mice , autopsy of these mice did not reveal any noticeable health problems suggesting that the early lethality of Htr2c-/Y; Pten+/- is caused by an increase in spontaneous epileptic seizures. An increased susceptibility to seizures has been reported in different models of Pten conditional deletion [39, 40, 51, 52], and ASD patients with a PTEN mutation can also present with epilepsy . Although we did not observe any spontaneous seizures in germline Pten+/- mice, it is possible that Htr2c mutation uncovers a susceptibility masked by the C57BL/6 background, which is normally seizure resistant . This enhancement of a seizure-prone phenotype in Htr2c-/Y; Pten+/- mice might seem surprising given that Pten physically interacts with 5-HT2cR to repress its activity , thus one might predict that Pten and Htr2c mutations would have opposing phenotypic effects. Indeed, we have demonstrated that pharmacological antagonism of 5-HT2cR with SB 242084 in Pten haploinsufficient mice is capable of restoring a preference for social investigation in a three-chamber social approach assay. In interpreting these results, it is worth considering that chronic treatment using a 5-HT2cR antagonist does not increase seizure susceptibility  and we did not observe any spontaneous seizures in Pten haploinsufficient mice treated with SB 242084, indicating that gross E/I balance is normal. Thus, it is possible that mutations in Htr2c and Pten may act in different cell types to influence E/I balance in a manner that is not recapitulated by pharmacological antagonism of 5-HT2cR. For example, mutations in Htr2c might result in decreased activity of GABAergic neurons [55–58] while Pten mutations result in increased activity of excitatory neurons , resulting in a synergistic elevation of E/I balance. Another possibility is that Pten and 5-HT2cR might have different periods and mechanisms of interaction: one complementary interaction in early development that sets up a later vulnerability to seizures, and another antagonistic interaction in mature circuitry that can reverse social behavioral deficits. Additionally, SB 242084 might rescue the social behavior deficits observed in Pten haploinsufficient mice through an anxiolytic effect, for example by modulating release of dopamine in the limbic system . While the precise mechanism remains to be discovered, the above data are consistent with the hypothesis that 5-HT2cR interacts with Pten to modulate both E/I balance and the circuitry influencing social behavior. Important issues to address in future work will be to determine the degree to which modulating 5-HT2cR is significant for the ASD-therapeutic effects of drugs such as risperidone, fluoxetine and olanzapine, which target a broad range of molecules, including 5-HT2cR.
We are grateful for generous support from Ms. Nancy Lurie Marks. This work was also supported by NIH grant number R01MH105610. We thank Mrs Trina L. Kemp for valuable administrative assistance, Drs. Amy E. Clipperton-Allen and Ori Cohen for useful advice on an early version of this manuscript, and Dr. Ronald Davis for suggestions about data analysis. We also thank Dr. Mriganka Sur for providing support and input for the 5-HT2cR pharmacological antagonist experiments presented here.
Conceived and designed the experiments: JS DL OK DP. Performed the experiments: JS DL OK DP. Analyzed the data: JS DL OK DP. Contributed reagents/materials/analysis tools: DP. Wrote the paper: JS DL OK DP.
- 1. Hay-Schmidt A. The evolution of the serotonergic nervous system. Proceedings Biological sciences / The Royal Society. 2000;267(1448):1071–9. pmid:10885511; PubMed Central PMCID: PMC1690648.
- 2. Canli T, Lesch KP. Long story short: the serotonin transporter in emotion regulation and social cognition. Nature neuroscience. 2007;10(9):1103–9. pmid:17726476.
- 3. Young SN, Leyton M. The role of serotonin in human mood and social interaction. Insight from altered tryptophan levels. Pharmacology, biochemistry, and behavior. 2002;71(4):857–65. pmid:11888576.
- 4. Higley JD, King ST Jr., Hasert MF, Champoux M, Suomi SJ, Linnoila M. Stability of interindividual differences in serotonin function and its relationship to severe aggression and competent social behavior in rhesus macaque females. Neuropsychopharmacology: official publication of the American College of Neuropsychopharmacology. 1996;14(1):67–76. pmid:8719031.
- 5. Lesch KP, Waider J. Serotonin in the modulation of neural plasticity and networks: implications for neurodevelopmental disorders. Neuron. 2012;76(1):175–91. pmid:23040814.
- 6. Kravitz EA, Huber R. Aggression in invertebrates. Current opinion in neurobiology. 2003;13(6):736–43. pmid:14662376.
- 7. Berg KA, Harvey JA, Spampinato U, Clarke WP. Physiological relevance of constitutive activity of 5-HT2A and 5-HT2C receptors. Trends in pharmacological sciences. 2005;26(12):625–30. pmid:16269190.
- 8. Olivier B, Molewijk HE, van der Heyden JA, van Oorschot R, Ronken E, Mos J, et al. Ultrasonic vocalizations in rat pups: effects of serotonergic ligands. Neuroscience and biobehavioral reviews. 1998;23(2):215–27. pmid:9884114.
- 9. Yu HJ, Yamaguchi A. 5-HT2C-like receptors in the brain of Xenopus laevis initiate sex-typical fictive vocalizations. Journal of neurophysiology. 2009;102(2):752–65. pmid:19474172; PubMed Central PMCID: PMC2724353.
- 10. Hackler EA, Turner GH, Gresch PJ, Sengupta S, Deutch AY, Avison MJ, et al. 5-Hydroxytryptamine2C receptor contribution to m-chlorophenylpiperazine and N-methyl-beta-carboline-3-carboxamide-induced anxiety-like behavior and limbic brain activation. The Journal of pharmacology and experimental therapeutics. 2007;320(3):1023–9. pmid:17138863.
- 11. Kennett GA, Wood MD, Bright F, Trail B, Riley G, Holland V, et al. SB 242084, a selective and brain penetrant 5-HT2C receptor antagonist. Neuropharmacology. 1997;36(4–5):609–20. pmid:9225286.
- 12. Dekeyne A, Denorme B, Monneyron S, Millan MJ. Citalopram reduces social interaction in rats by activation of serotonin (5-HT)(2C) receptors. Neuropharmacology. 2000;39(6):1114–7. pmid:10727723.
- 13. Christianson JP, Ragole T, Amat J, Greenwood BN, Strong PV, Paul ED, et al. 5-hydroxytryptamine 2C receptors in the basolateral amygdala are involved in the expression of anxiety after uncontrollable traumatic stress. Biological psychiatry. 2010;67(4):339–45. pmid:19914601; PubMed Central PMCID: PMC3278236.
- 14. Nakatani J, Tamada K, Hatanaka F, Ise S, Ohta H, Inoue K, et al. Abnormal behavior in a chromosome-engineered mouse model for human 15q11-13 duplication seen in autism. Cell. 2009;137(7):1235–46. pmid:19563756; PubMed Central PMCID: PMC3710970.
- 15. Allan AM, Liang X, Luo Y, Pak C, Li X, Szulwach KE, et al. The loss of methyl-CpG binding protein 1 leads to autism-like behavioral deficits. Human molecular genetics. 2008;17(13):2047–57. pmid:18385101; PubMed Central PMCID: PMC2700628.
- 16. Anastasio NC, Gilbertson SR, Bubar MJ, Agarkov A, Stutz SJ, Jeng Y, et al. Peptide inhibitors disrupt the serotonin 5-HT2C receptor interaction with phosphatase and tensin homolog to allosterically modulate cellular signaling and behavior. The Journal of neuroscience: the official journal of the Society for Neuroscience. 2013;33(4):1615–30. pmid:23345234; PubMed Central PMCID: PMC3711763.
- 17. Ji SP, Zhang Y, Van Cleemput J, Jiang W, Liao M, Li L, et al. Disruption of PTEN coupling with 5-HT2C receptors suppresses behavioral responses induced by drugs of abuse. Nature medicine. 2006;12(3):324–9. pmid:16474401.
- 18. Cully M, You H, Levine AJ, Mak TW. Beyond PTEN mutations: the PI3K pathway as an integrator of multiple inputs during tumorigenesis. Nature reviews Cancer. 2006;6(3):184–92. pmid:16453012.
- 19. Butler MG, Dasouki MJ, Zhou XP, Talebizadeh Z, Brown M, Takahashi TN, et al. Subset of individuals with autism spectrum disorders and extreme macrocephaly associated with germline PTEN tumour suppressor gene mutations. Journal of medical genetics. 2005;42(4):318–21. pmid:15805158; PubMed Central PMCID: PMC1736032.
- 20. Goffin A, Hoefsloot LH, Bosgoed E, Swillen A, Fryns JP. PTEN mutation in a family with Cowden syndrome and autism. American journal of medical genetics. 2001;105(6):521–4. pmid:11496368.
- 21. Herman GE, Butter E, Enrile B, Pastore M, Prior TW, Sommer A. Increasing knowledge of PTEN germline mutations: Two additional patients with autism and macrocephaly. American journal of medical genetics Part A. 2007;143A(6):589–93. pmid:17286265.
- 22. Herman GE, Henninger N, Ratliff-Schaub K, Pastore M, Fitzgerald S, McBride KL. Genetic testing in autism: how much is enough? Genetics in medicine: official journal of the American College of Medical Genetics. 2007;9(5):268–74. pmid:17505203.
- 23. Brennan TJ, Seeley WW, Kilgard M, Schreiner CE, Tecott LH. Sound-induced seizures in serotonin 5-HT2c receptor mutant mice. Nature genetics. 1997;16(4):387–90. pmid:9241279.
- 24. Tecott LH, Sun LM, Akana SF, Strack AM, Lowenstein DH, Dallman MF, et al. Eating disorder and epilepsy in mice lacking 5-HT2c serotonin receptors. Nature. 1995;374(6522):542–6. pmid:7700379.
- 25. Bourgeron T. A synaptic trek to autism. Current opinion in neurobiology. 2009;19(2):231–4. pmid:19545994.
- 26. Markram K, Markram H. The intense world theory—a unifying theory of the neurobiology of autism. Frontiers in human neuroscience. 2010;4:224. pmid:21191475; PubMed Central PMCID: PMC3010743.
- 27. Rubenstein JL. Three hypotheses for developmental defects that may underlie some forms of autism spectrum disorder. Current opinion in neurology. 2010;23(2):118–23. pmid:20087182.
- 28. Rubenstein JL, Merzenich MM. Model of autism: increased ratio of excitation/inhibition in key neural systems. Genes, brain, and behavior. 2003;2(5):255–67. pmid:14606691.
- 29. Vattikuti S, Chow CC. A computational model for cerebral cortical dysfunction in autism spectrum disorders. Biological psychiatry. 2010;67(7):672–8. pmid:19880095; PubMed Central PMCID: PMC3104404.
- 30. Yizhar O, Fenno LE, Prigge M, Schneider F, Davidson TJ, O'Shea DJ, et al. Neocortical excitation/inhibition balance in information processing and social dysfunction. Nature. 2011;477(7363):171–8. pmid:21796121; PubMed Central PMCID: PMC4155501.
- 31. Xu Y, Jones JE, Kohno D, Williams KW, Lee CE, Choi MJ, et al. 5-HT2CRs expressed by pro-opiomelanocortin neurons regulate energy homeostasis. Neuron. 2008;60(4):582–9. pmid:19038216; PubMed Central PMCID: PMC2631191.
- 32. Podsypanina K, Ellenson LH, Nemes A, Gu J, Tamura M, Yamada KM, et al. Mutation of Pten/Mmac1 in mice causes neoplasia in multiple organ systems. Proceedings of the National Academy of Sciences of the United States of America. 1999;96(4):1563–8. pmid:9990064; PubMed Central PMCID: PMC15517.
- 33. Clipperton-Allen AE, Page DT. Pten haploinsufficient mice show broad brain overgrowth but selective impairments in autism-relevant behavioral tests. Human molecular genetics. 2014;23(13):3490–505. pmid:24497577.
- 34. Page DT, Kuti OJ, Prestia C, Sur M. Haploinsufficiency for Pten and Serotonin transporter cooperatively influences brain size and social behavior. Proceedings of the National Academy of Sciences of the United States of America. 2009;106(6):1989–94. pmid:19208814; PubMed Central PMCID: PMC2644151.
- 35. Curia G, Gualtieri F, Bartolomeo R, Vezzali R, Biagini G. Resilience to audiogenic seizures is associated with p-ERK1/2 dephosphorylation in the subiculum of Fmr1 knockout mice. Frontiers in cellular neuroscience. 2013;7:46. pmid:23630463; PubMed Central PMCID: PMC3635025.
- 36. Brodkin ES, Hagemann A, Nemetski SM, Silver LM. Social approach-avoidance behavior of inbred mouse strains towards DBA/2 mice. Brain research. 2004;1002(1–2):151–7. pmid:14988045.
- 37. Nonogaki K, Abdallah L, Goulding EH, Bonasera SJ, Tecott LH. Hyperactivity and reduced energy cost of physical activity in serotonin 5-HT(2C) receptor mutant mice. Diabetes. 2003;52(2):315–20. pmid:12540602.
- 38. Browning RA, Lanker ML, Faingold CL. Injections of noradrenergic and GABAergic agonists into the inferior colliculus: effects on audiogenic seizures in genetically epilepsy-prone rats. Epilepsy research. 1989;4(2):119–25. pmid:2792066.
- 39. Kwon CH, Luikart BW, Powell CM, Zhou J, Matheny SA, Zhang W, et al. Pten regulates neuronal arborization and social interaction in mice. Neuron. 2006;50(3):377–88. pmid:16675393; PubMed Central PMCID: PMC3902853.
- 40. Zhou J, Blundell J, Ogawa S, Kwon CH, Zhang W, Sinton C, et al. Pharmacological inhibition of mTORC1 suppresses anatomical, cellular, and behavioral abnormalities in neural-specific Pten knock-out mice. The Journal of neuroscience: the official journal of the Society for Neuroscience. 2009;29(6):1773–83. pmid:19211884; PubMed Central PMCID: PMC3904448.
- 41. Kandel ER, Spencer WA. Excitation and inhibition of single pyramidal cells during hippocampal seizure. Experimental neurology. 1961;4:162–79. pmid:13751139.
- 42. Stief F, Zuschratter W, Hartmann K, Schmitz D, Draguhn A. Enhanced synaptic excitation-inhibition ratio in hippocampal interneurons of rats with temporal lobe epilepsy. The European journal of neuroscience. 2007;25(2):519–28. pmid:17284194.
- 43. Tuchman R, Cuccaro M, Alessandri M. Autism and epilepsy: historical perspective. Brain & development. 2010;32(9):709–18. pmid:20510557.
- 44. Makinodan M, Rosen KM, Ito S, Corfas G. A critical period for social experience-dependent oligodendrocyte maturation and myelination. Science. 2012;337(6100):1357–60. pmid:22984073; PubMed Central PMCID: PMC4165613.
- 45. Baskerville TA, Douglas AJ. Dopamine and oxytocin interactions underlying behaviors: potential contributions to behavioral disorders. CNS neuroscience & therapeutics. 2010;16(3):e92–123. pmid:20557568.
- 46. Choleris E, Clipperton-Allen AE, Gray DG, Diaz-Gonzalez S, Welsman RG. Differential effects of dopamine receptor D1-type and D2-type antagonists and phase of the estrous cycle on social learning of food preferences, feeding, and social interactions in mice. Neuropsychopharmacology: official publication of the American College of Neuropsychopharmacology. 2011;36(8):1689–702. pmid:21525863; PubMed Central PMCID: PMC3138658.
- 47. Enter D, Colzato LS, Roelofs K. Dopamine transporter polymorphisms affect social approach-avoidance tendencies. Genes, brain, and behavior. 2012;11(6):671–6. pmid:22494359.
- 48. Ernst M, Zametkin AJ, Matochik JA, Pascualvaca D, Cohen RM. Low medial prefrontal dopaminergic activity in autistic children. Lancet. 1997;350(9078):638. pmid:9288051.
- 49. Nakamura K, Sekine Y, Ouchi Y, Tsujii M, Yoshikawa E, Futatsubashi M, et al. Brain serotonin and dopamine transporter bindings in adults with high-functioning autism. Archives of general psychiatry. 2010;67(1):59–68. pmid:20048223.
- 50. Bubar MJ, Cunningham KA. Distribution of serotonin 5-HT2C receptors in the ventral tegmental area. Neuroscience. 2007;146(1):286–97. pmid:17367945; PubMed Central PMCID: PMC1939890.
- 51. Backman SA, Stambolic V, Suzuki A, Haight J, Elia A, Pretorius J, et al. Deletion of Pten in mouse brain causes seizures, ataxia and defects in soma size resembling Lhermitte-Duclos disease. Nature genetics. 2001;29(4):396–403. pmid:11726926.
- 52. Kwon CH, Zhu X, Zhang J, Baker SJ. mTor is required for hypertrophy of Pten-deficient neuronal soma in vivo. Proceedings of the National Academy of Sciences of the United States of America. 2003;100(22):12923–8. pmid:14534328; PubMed Central PMCID: PMC240720.
- 53. Conti S, Condo M, Posar A, Mari F, Resta N, Renieri A, et al. Phosphatase and tensin homolog (PTEN) gene mutations and autism: literature review and a case report of a patient with Cowden syndrome, autistic disorder, and epilepsy. Journal of child neurology. 2012;27(3):392–7. pmid:21960672.
- 54. Fuller JL, Sjursen FH Jr. Audiogenic seizures in eleven mouse strains. The Journal of heredity. 1967;58(3):135–40. pmid:6055327.
- 55. Abi-Saab WM, Bubser M, Roth RH, Deutch AY. 5-HT2 receptor regulation of extracellular GABA levels in the prefrontal cortex. Neuropsychopharmacology: official publication of the American College of Neuropsychopharmacology. 1999;20(1):92–6. pmid:9885788.
- 56. Cremers TI, Rea K, Bosker FJ, Wikstrom HV, Hogg S, Mork A, et al. Augmentation of SSRI effects on serotonin by 5-HT2C antagonists: mechanistic studies. Neuropsychopharmacology: official publication of the American College of Neuropsychopharmacology. 2007;32(7):1550–7. pmid:17203017.
- 57. Liu R, Jolas T, Aghajanian G. Serotonin 5-HT(2) receptors activate local GABA inhibitory inputs to serotonergic neurons of the dorsal raphe nucleus. Brain research. 2000;873(1):34–45. pmid:10915808.
- 58. Di Giovanni G, Di Matteo V, La Grutta V, Esposito E. m-Chlorophenylpiperazine excites non-dopaminergic neurons in the rat substantia nigra and ventral tegmental area by activating serotonin-2C receptors. Neuroscience. 2001;103(1):111–6. pmid:11311791.
- 59. Luikart BW, Schnell E, Washburn EK, Bensen AL, Tovar KR, Westbrook GL. Pten knockdown in vivo increases excitatory drive onto dentate granule cells. The Journal of neuroscience: the official journal of the Society for Neuroscience. 2011;31(11):4345–54. pmid:21411674; PubMed Central PMCID: PMC3113533.
- 60. Di Matteo V, Di Giovanni G, Di Mascio M, Esposito E. SB 242084, a selective serotonin2C receptor antagonist, increases dopaminergic transmission in the mesolimbic system. Neuropharmacology. 1999;38(8):1195–205. pmid:10462132.