The clinical applicability of brain oxygenation mapping using the MOBILE (Mapping of Oxygen By Imaging Lipids relaxation Enhancement) magnetic resonance (MR) technique was assessed in the clinical setting of normal brain and of acute cerebral ischemia as a founding proof-of-concept translational study. Changes in the oxygenation level within healthy brain tissue can be detected by analyzing the spin-lattice proton relaxation (‘Global T1’ combining water and lipid protons) because of the paramagnetic properties of molecular oxygen. It was hypothesized that selective measurement of the relaxation of the lipid protons (‘Lipids T1’) would result in enhanced sensitivity of pO2 mapping because of higher solubility of oxygen in lipids than in water, and this was demonstrated in pre-clinical models using the MOBILE technique. In the present study, 12 healthy volunteers and eight patients with acute (48–72 hours) brain infarction were examined with the same clinical 3T MR system. Both Lipids R1 (R1 = 1/T1) and Global R1 were significantly different in the infarcted area and the contralateral unaffected brain tissue, with a higher statistical significance for Lipids R1 (median difference: 0.408 s-1; p<0.0001) than for Global R1 (median difference: 0.154 s-1; p = 0.027). Both Lipids R1 and Global R1 values in the unaffected contralateral brain tissue of stroke patients were not significantly different from the R1 values calculated in the brain tissue of healthy volunteers. The main limitations of the present prototypic version of the MOBILE sequence are the long acquisition time (4 min), hampering robustness of data in uncooperative patients, and a 2 mm slice thickness precluding accurate measurements in small infarcts because of partial volume averaging effects.
Citation: Colliez F, Safronova MM, Magat J, Joudiou N, Peeters AP, Jordan BF, et al. (2015) Oxygen Mapping within Healthy and Acutely Infarcted Brain Tissue in Humans Using the NMR Relaxation of Lipids: A Proof-Of-Concept Translational Study. PLoS ONE 10(8): e0135248. https://doi.org/10.1371/journal.pone.0135248
Editor: Johannes Boltze, Fraunhofer Institute for Cell Therapy and Immunology, GERMANY
Received: February 2, 2015; Accepted: July 20, 2015; Published: August 12, 2015
Copyright: © 2015 Colliez et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Data Availability: All relevant data are within the paper.
Funding: This work was supported by the FRS-FNRS (7.4599.11 )(http://www.fnrs.be/), the Fournier-Majoie Foundation(BG, http://www.fournier-majoie.org/en), the Fonds Joseph Maisin (BG, http://www.centreducancer.be/fr/show/index/section/8/page/88), the Plan Cancer (Belgian Ministry of Health, KPC_29_027)(TD, http://health.belgium.be/eportal/Healthcare/Specialisedcare/CancerPlan/19080671?ie2Term=plan%20cancer?&fodnlang=fr#.VMYc5i6LpOh), and the Fondation contre le Cancer (C/2014/255) (BG, http://www.cancer.be/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The patent filled by BFJ, BG and JM (Patent application: 11163573.6 and PCT/EP2012/057237 about the concept of measuring lipid relaxation to assess tissue oxygenation) does not alter the adherence to all PLOS ONE policies on sharing data and materials. There is no other competing interest to declare.
Mapping brain oxygenation is a key challenge in the clinical workup of many cerebral disorders, including acute/chronic ischemia, neoplastic processes, and a wide range of neurodegenerative disorders [1,2]. However, in vivo pO2 measurement within the brain remains an unsolved issue in clinical practice. Efficient methods for brain oxygen mapping in a clinical setting should satisfy challenging criteria such as noninvasiveness, non-toxicity (at least low radiation exposure), sufficient spatial resolution, accurate quantification of pO2 levels, and widespread availability, which are not by any means satisfied by currently available methods. Nowadays the reference technique for whole-brain mapping of hypoxia is Positron Emission Tomography (PET), using nitroimidazole-derived tracers which accumulate within hypoxic areas . But costs, reduced availability, and radiation exposure limit its use in routine practice. Magnetic resonance (MR) techniques using endogenous contrast agents (CA) could be alternatives to PET for oxygen levels measurements [4–6]. Endogenous sources of O2-driven tissue contrast in MR Imaging include variations in T2* (effective transversal relaxation time) and T1 (spin-lattice or longitudinal relaxation time) . T2* is sensitive to the deoxyhemoglobin (Hb)/oxyhemoglobin (HbO2) ratio within vessels . T2* mapping, also referred to as ‘functional MR imaging’ or ‘blood oxygen level dependent (BOLD) MR imaging ‘is mainly sensitive to variations in intravascular oxygenation and has been successfully applied to monitor changes in the oxygen level within the vascular compartment, e.g. in neuronal activation. But BOLD-MRI has demonstrated significant limitations in terms of quantitative relationships between response signal intensity and true pO2 changes within tissues, and in terms of sensitivity to changes in total hemoglobin content . In addition, the BOLD-based approach to brain oxygenation measurement has specifically demonstrated additional limitations such as excessive dependence on B0 inhomogeneities, water diffusion, and ultra-structural properties of the capillary network . T1 is sensitive to dissolved molecular oxygen within tissues, thereby acting more as a tissular T1- shortening paramagnetic CA than an intravascular one . Analysis of proton T1 relaxation induced by molecular oxygen has shown that it can be used to monitor changes in tissue oxygenation in solid tumors and several normal tissues such as the spleen, liver, renal cortex, and skeletal muscles [12,13]. The technique referred to as ‘Oxygen-enhanced MRI’ measures the proton T1 relaxation time, which is mainly influenced by the water protons. We called this measurement ‘Global T1’ in the present study. Recently, the MOBILE (Mapping of Oxygen by Imaging Lipids relaxation Enhancement) sequence was developed to increase the sensitivity of T1 measurements by selectively recording the T1 of the lipids, a specific measurement we called ‘Lipids T1‘. This method is conceptually based on the higher solubility of oxygen in lipids than in water and the demonstrated ability to monitor changes in tissue oxygenation in brain, muscles, liver and tumors in a preclinical setting . Moreover, a significant correlation has been established between Lipids R1 (with R1 = 1/ T1) values (obtained with MOBILE) and pO2 values recorded with Electron Paramagnetic Resonance , suggesting the quantitative value of MOBILE in this pre-clinical setting. In a translational ‘proof-of-concept’ study, we have attempted to demonstrate the applicability of MOBILE in healthy volunteers and in acute stroke patients using standard clinical MR equipment. In the second cohort we investigated whether it is capable of detecting brain oxygen deprivation in the paradigmatic pathological condition of acute decrease in oxygen supply within a lipid-rich biological tissue.
Materials and Methods
Healthy volunteer and patient cohorts
After the study protocol was cleared by the institutional Ethics Committee (Commission d’Ethique Biomédicale Hospitalo-Facultaire, Medical Faculty of Université Catholique de Louvain, Brussels, Ref CE 2011/21JUIL/255Belgium no. 403201111606) an initial normative cohort of 12 healthy volunteers was examined after giving their written informed consent. A standardized MR imaging protocol including a FLAIR sequence together with Global R1 (= 1/T1), Lipids R1, and R2* (= 1/T2*) sequences was applied to all of them using the same 3T clinical MR system. Eighteen patients presenting with acute ischemic stroke were then recruited. All potential candidates for inclusion were duly informed that the study protocol was investigational, without impact on either individual treatment strategy or prognosis, and that no parenteral contrast agent (CA) would be administered. All of them gave their written consent. Commitment was paid to avoid any interference with the individual therapeutic strategy.
Criteria for inclusion were: i: the presence of a large infarction on the admission brain CT or MR workup; ii: feasibility of the study MR examination within a 2- to 3-day delay after stroke onset; iii: absence of prior thrombolytic therapy, to minimize risk of confounding micro-hemorrhages; and iv: informed consent for the study protocol obtained from patients and/or a decision-maker substitute.
Criteria for exclusion were: i: bleeding, mainly secondary hemorrhagic transformation of initially ischemic stroke; ii: presumptive poor cooperation and/or confusion/agitation/incoercible motion.
All examinations were performed on the same clinical 3-Tesla MR system (Achieva 3T Release 3.2, Philips Healthcare, Best, The Netherlands) using a 32-channel receiver head coil. A standardized protocol which included whole-brain covering fluid-attenuated inversion-recovery (FLAIR), Echo-Planar Imaging gradient echo T2 (EPI-T2*), and diffusion-weighted imaging (DWI) sequences together with the three investigational Global R1, Lipids R1 (MOBILE), and R2* acquisitions was applied to all volunteers and patients. Since all three of the latter sequences allowed data acquisition at only one slice location, the ‘best’ slice location was defined as that displaying the largest infarcted zone without hemorrhagic transformation on morphologic FLAIR/DWI/EPI-T2* images. Parameters of conventional sequences were as follows: (i) FSE-FLAIR: TR: 10,000 ms; Ti: 2,800 ms; TE:125 ms; Sense factor: 1.5; slice thickness: 4 mm; (ii) DWI: TR: 2907 ms; TE: 55 ms; Sense factor: 2; b factors: 0–1000 mm2/s; slice thickness: 4 mm; (iii) EPI-T2* sequence: TR: 1461 ms; TE: 16.11 ms; EPI factor: 9; Flip angle: 40°; slice thickness: 4 mm.
Parameters of investigational sequences were as follows:
- -. for Global R1 measurements: a ‘Look Locker’ T1 TFE sequence with TR/TE/flip angle/TFE/NSA = 3.467 ms/1.45 ms/5°/10/1 was applied for an acquisition time (AT) of 10s to obtain one 20mm-thick slice covering a FOV of 183x230 mm with a matrix size of 802 resulting in a voxel size of 3.91x5.08x20 mm.
- -. for Lipids R1 measurements (MOBILE): a similar sequence was used with the addition of a 90° SPIR (SPectral saturation by Inversion Recovery) pre-pulse to spoil water with a bandwidth of 300Hz centered on the water peak; 38 images averaged 30 times with metrics similar to those for the previous acquisition were obtained for an AT of 4 min.
- -. for R2* measurements: a multi Fast Field Echo (mFFE) sequence was performed with TR/flip angle/echoes = 250ms/18°/15 to acquire 32 4mm-slices for a total acquisition time of 41.8 s. The FOV was 230X183 and the matrix size 3202, resulting in a voxel size of 0.9X1.12X4 mm.
Data were processed using self-programmed Matlab scripts (MatLab Release 2013a, The MathWorks Inc., Natick, MA, USA). Parametric maps were reconstructed for Global R1, Lipids R1, and R2* data. Two-level filtering was applied for T1 measurements: i: T1 values were deleted when the relative error made on this value exceeded 30%; and ii: T1 values below 100 ms were considered not characteristic for lipid protons and therefore were also deleted . For each patient, the infarcted area was delineated on FLAIR images by manual planimetric contouring around the hyperintense region and a contralateral mirror- ROI within unaffected brain tissue was manually generated. To be consistent with previous reports dealing with oxygen-enhanced MRI [12–15], the results were given in terms of R1 values instead of T1. Both ROIs were electronically superimposed on parametric R1 and R2*maps. Median values of relaxation rates within the two ROIs (infarct versus mirror-ROI) were calculated. In volunteers, each cerebral hemisphere was analyzed as a whole using the same routine.
Analysis of differences between ischemic brain tissue and unaffected contralateral brain tissue was made for each parameter using the GraphPad Prism Software (GraphPad Software Inc., Release 5, San Diego, CAL, USA). Normality of datasets was assessed using the D’Agostino & Pearson test with p<0.05 rejecting the normality. The parametric paired t-test in cases of normal distribution of the R1 and R2* values and the non-parametric Wilcoxon test for matched pairs in cases of non-normal distribution were performed to compare medians obtained from ischemic and non-ischemic areas. Similarly parametric unpaired t-tests and non-parametric Mann-Whitney tests were performed to compare median values within unaffected mirror ROIs in stroke patients versus normal brain in healthy volunteers.
Study design: volunteer and patient recruitment and data analysis
Twelve volunteers were recruited to obtain a normative database of basal Global R1, Lipids R1, (MOBILE) and R2* values in healthy brain tissue. Thereafter, 18 consecutive patients presenting with acute (48–72 hours after onset of symptoms) brain ischemic stroke were recruited. Both groups were examined after giving informed consent according to the institutional Ethics Committee (EC) requirements. Seven patients had to be removed from the database because of uncontrolled motion, one for improper infarct analysis because of a small infarction of which analysis was degraded by partial volume averaging with cerebral spinal fluid (CSF), and two patients with lacunar infarcts including less than 10 voxels from which accurate R1 measurement was calculable. As a result, only 8 patients were finally included in the study. Among them, one patient had two concomitant acute infarcts resulting in 9 ischemic areas available for analysis, 8 of which were located in the supratentorial space and one in the posterior fossa. None of the patients had received thrombolytic therapy or had hemorrhagic transformation of infarcted brain tissue at the time of the MR examination.
Typical maps & analysis
Fig 1 displays a morphological axial FLAIR image (Fig 1b) together with parametric maps for Global R1 (Fig 1b), Lipids R1 (MOBILE) (Fig 1c), and R2*(Fig 1d) in a similar slice location. Analysis was performed on manually delineated regions of interest (ROIs) contouring the infarcted area and the mirrored area within the unaffected contralateral hemisphere on the morphological image, as illustrated by the overlay of the two ROIs from the MOBILE R1 map of the FLAIR image (Fig 1e). Individual histogram analysis (Fig 1f–1h) demonstrated a significant shift to lower values of the medians within infarcted tissue when compared to contralateral unaffected brain tissue for Global R1 values (Fig 1f), Lipids R1 values (Fig 1g), and R2* values (Fig 1h). The statistical results for each patient are presented in Table 1.
Axial transverse FLAIR image (1.a) through centrum semiovale showing hyperintensity within acutely infarcted whole territory of the right middle cerebral artery (MCA), and corresponding global R1-mapped image (1.b), lipids R1-mapped image (MOBILE) (1.c) and R2*-mapped image (1.d) in a similar slice location. Image 1.e: overlay of the two ROIs (right-sided contour of infarction and left-sided mirrored one) from the MOBILE map (1.b) on the FLAIR image (1.a). Images 1b-1e have been smoothed using the ‘Gaussian Blur’ function (radius of 2 pixels) of the GIMP software (GIMP 2, GNU Image Manipulation Program, GPLv3). Histograms of global R1 (1.f), lipids R1 (1.g) and R2* (1.h) of the same patient demonstrating significant shift of the medians to lower values for the stroke area. Differences of 0.111 s-1, 0.536 s-1 and 6.09 s-1 are observed for global R1, lipids R1 and R2*, respectively.
Pooled data analysis of the whole patient group
Pooled data histogram analysis of the 9 infarcts (Fig 2) confirmed the significant shift of the medians within ischemic tissue to lower values for Global R1and for Lipids R1 when compared to unaffected brain tissue. A 0.154 s-1 mean difference was calculated between medians for Global R1 within infarct ROIs and contralateral mirrored ROIs, resulting in a p value = 0.027. A 0.408 s-1 difference was calculated for Lipids R1, resulting in an improved p value < 0.0001 (Fig 2b). A statistically significant shift to lower values was also observed for the medians of the R2* values upon group analysis, with a p value of 0.0195, in an overall range similar to that for individual analyses.
Histogram and scatter-plot of pooled 9 infarcted areas (‘stroke’) versus unaffected mirror areas (‘control’). 3.a: Histograms demonstrated that the shift to lower values of lipids R1 in stroke areas (0.408 s-1; p<0.0001) is more pronounced than the corresponding shift observed with global R1 values (0.154 s-1; p<0.027). 3.b: Scatter plots demonstrated a greater difference between medians for lipids R1 at 0.409 s-1 with p <0.0001 (in orange) than for global R1 at 0.151 s-1 with p<0.027 (in blue).
Comparison between stroke patient group and normative healthy volunteer cohort
Fig 3 shows a close correlation between medians of contralateral mirror ROIs within stroke patients’ unaffected brain tissue (‘control’) and healthy brain hemispheres of volunteers (‘volunteers’) for Global R1, Lipids R1, and R2* values (p > 0.10).
Global R1 plots (3.a), Lipids R1 plots (3.b) and R2* plots (3.c) demonstrated a statistically significant difference between infarcts and unaffected mirror-ROIs in patients. In turn, no difference was observed for all modalities between the values obtained in patient mirror-ROIs and healthy brain tissue in volunteers.
In vivo pO2 measurement within tissues is a crucial and unsolved challenge in clinical practice. Standard oximetry methods involve either traumatic (deeply implanted electrodes) or irradiating (PET) techniques, routine use of which therefore seem precluded. This study investigated whether a new non-invasive and innocuous technique could highlight hypoxia in the paradigmatic condition of acute ischemic stroke. The MOBILE sequence using molecular oxygen as an endogenous CA was designed for noninvasive measurements of tissue R1 . The ability of the technique to monitor an increase in brain oxygenation during a hyperoxic challenge  as well as positive and negative changes in oxygenation in preclinical tumor models has been previously demonstrated . This preliminary translational implementation was attempted in the brain because of the exquisite dependence of brain tissue viability on O2 supply and because of the elevated lipid content of cerebral tissue. Moreover, stroke is a paradigmatic condition in which both normoxic and hypoxic areas are displayed on the same axial transverse slice of the brain.
The results confirmed the ability of MOBILE to highlight hypoxia in a stroke area when compared to a normoxic brain area. R1 values recorded in ischemic areas were significantly lower than those calculated within contralateral unaffected areas for both Global R1 (which was consistent with existing literature [17,18]) and Lipids R1. Although both methods seemed able to distinguish hypoxic from normoxic areas, a slightly more significant performance was observed for selective lipid enhancement. The true oxygenation status within injured brain tissue could not be assessed quantitatively by measuring differences between Lipids R1 values within stroke vs. normoxic mirrored areas, thereby precluding diagnostic accuracy and/or prediction of patient outcome. Indeed, the difference between Lipids R1 values measured in stroke areas and Lipids R1 values measured in contralateral normoxic brain was not sufficient to predict the oxygenation status from a Lipids R1 measurement: in Fig 2, the lowest values of Lipids R1 obtained in the ‘control’ areas in fact overlapped with the highest values measured in ‘stroke’ areas. This was also observed with conventional R1 measurements. Several studies have shown that Global T1 measurements are strongly influenced by water content [19,20]. Consequently the Global T1 values calculated within the stroke area result from the opposing influences of edema vs. hypoxia. Since the MOBILE sequence included a saturation pulse spoiling the water signal, the Lipids T1 should be less influenced by the change in water content within the infarcted area, even if we cannot neglect this parameter. In addition to the water content, it has been demonstrated that blood flow [12,21], basal oxygenation and basal saturation of hemoglobin  are other factors influencing the relaxation rate measurement. From our results, we cannot exclude that those parameters may have influenced the T1 measurements. Nevertheless, preclinical studies have shown that the MOBILE technique is sensitive to positive and negative changes in oxygenation, and the quantitative capabilities of the technique have been demonstrated in a preclinical setting . Consequently, despite the multiple factors influencing Lipids R1 values, the difference between Lipids R1 values measured in hypoxic areas (infarcted brain) and Lipids R1 values measured in contralateral unaffected brain were considered to mainly reflect a difference in tissue oxygenation. To assess the reliability of Lipids R1 measurements, we compared R1 values within contralateral unaffected brain tissue of stroke patients to those recorded in healthy brain tissue of volunteers and failed to demonstrate significant differences between the two groups. This correspondence suggested the consistency of MOBILE-based brain pO2 mapping in distinguishing hypoxic from normoxic areas displayed in the same slice location in the axial-transverse plane. However, further work needs to be done to test the repeatability and to investigate the quantitative aspect of MOBILE in a clinical setting. Each patient in the study was his/her own control since the symmetrical anatomy of the brain had the advantage of allowing accurate comparison between ischemic tissue and unaffected tissue in a similar area within the contralateral hemisphere.
The present version of the MOBILE sequence suffered from practical limitations at acquisition. In particular, a 4-minute acquisition time precluded its use in uncooperative and/or neurologically disabled patients, and a 20-mm slice thickness resulted in partial volume averaging artifacts which were deleterious e.g. in the upper brain areas where subcutaneous fat superseded the signal from brain lipids. Similarly, MOBILE did not allow accurate measurements in juxtaventricular regions because of partial averaging effects. In addition, significant dispersion of Lipids R1 measurements was observed in the processing phase within the unaffected contralateral mirror ROI in patients or within healthy brain tissue in volunteers. Finally, fitting was not possible in some voxels, resulting in a loss of information (6% to 37% of voxels were excluded from the MOBILE analysis after the application of the two filters), which constituted a synergistic penalty with partial averaging for small infarct analysis.
A reduction in the median values of R2* was also observed when using BOLD-MRI to compare ischemic and non-ischemic tissue. This feature may sound paradoxical, since many reports have highlighted a shift to higher values of R2* values in hypoxic areas [22,23]. However, a few hours after initiation of the stroke process, the briefly delayed onset of vasogenic edema increases T2* values independently of blood oxygenation effects, resulting in a decrease in R2*. Moreover, electron-nuclear interactions between neighboring atoms give rise to a dissipative relaxation mechanism described by T2 which is closely linked to T2* . Thus far, it has been expected that a shift to lower values of R2* would be recorded within infarcts in a cohort of patients who were examined between 48 to 72 hours after stroke onset. This difference might appear to be more accurate than differences recorded with Lipids R1 or Global R1measurements. But R1 and R2* measurements do not reflect similar pathophysiological sub-processes, since R1 globally assesses tissue oxygenation and R2* mainly assesses intravascular imbalance of the ratio between oxyhemoglobin and deoxyhemoglobin, which indirectly reflects insufficient O2 supply. R1 and R2* are therefore hypothesized to provide complementary information.
Several quantitative magnetic resonance techniques are available to measure tissue oxygenation. Electron Paramagnetic Resonance (EPR) allows real-time monitoring of oxygenation, but it is an invasive method requiring the injection of a paramagnetic probe . The relaxation of the paramagnetic probe quantitatively reflects the partial pressure of oxygen. However, EPR is not widely available, has limited depth of efficiency, and only allows spectroscopic measurement, without image acquisition. 19F-MRI is a noninvasive method able to follow positive and negative changes in oxygenation after the administration of an emulsion of perfluorocarbon [25,26]. However, to our knowledge, there is no study reporting the clinical application of 19F-MRI since the perfluorocarbons are still awaiting FDA approval. The mqBOLD method should probably be the most relevant quantitative technique available for clinical implementation. This method allows quantitative mapping of tissue oxygenation by combining information provided by standard sequences aimed at measuring the blood volume fraction, the field inhomogeneities and the tissue T2 before and after the administration of a contrast agent . mqBOLD has already been successfully applied in gliomas and stroke models . Tissue pO2 estimates obtained in a non-traumatic and innocuous way using the MOBILE sequence running on routine clinical equipment could add to the brain perfusion information which reflects the tissue vascularization more than the true oxygenation level. The exploratory implementation of MOBILE reported here in a clinical setting was not designed to provide diagnostic accuracy in cases of hyperacute stroke but to assess whether MOBILE could demonstrate hypoxia within infarcted brain areas as a proof- of-concept in humans, as it is ethically unfeasible to make direct measurements in normal brain tissue. With the added support of pre-clinical published results, we may then extrapolate the use of MOBILE in tumor conditions. The application of MOBILE in patients with brain tumors would be of great interest in planning radiotherapy, since tumor hypoxia induces radioresistance, and since killing hypoxic tumor tissues requires the administration of a radiation dose 3 times higher than that in normoxic tumor tissue. The MOBILE technique could be therefore a sensitive and noninvasive method for radiotherapy planning, delineating the tumor hypoxic regions that should benefit from a boost of irradiation. Further studies will investigate this application.
The initial steps of clinical applicability and consistency of results have been successfully completed. Further refinements of the sequence yielding higher spatial resolution and enhanced accuracy of quantitative results are being implemented.
In conclusion, feasibility of MR-based in vivo brain pO2 mapping through calculation of the relaxation effect of molecular O2 on water and lipid protons was demonstrated in both normal cerebral tissue and acutely infarcted tissue using a clinical 3-Tesla equipment. The MOBILE technique selectively sampling relaxation effects of O2 on lipid protons was able to pinpoint hypoxia in infarcted areas compared to normoxic brain tissue. The availability of such an innocuous and repeatable technique for tissue pO2 mapping on routine MR systems opens up extensive prospects for investigation in clinical practice.
Conceived and designed the experiments: BG BFJ TD. Performed the experiments: FC JM. Analyzed the data: FC MS NJ. Contributed reagents/materials/analysis tools: MS JM AP BG TD. Wrote the paper: FC MS JM NJ BFJ BG TD.
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