Santos Soprano A, Abe VY, Costa Smetana JH and Benedetti CE: Citrus MAF1, a Repressor of RNA Polymerase III, Binds the Xanthomonas citri Canker Elicitor PthA4 and Suppresses Citrus Canker Development. Plant Physiology http://dx.doi.org/10.1104/pp.113.224642.
(A) Citrus preys fused to yeast GAL4-AD (GAL4AD-prey) or control plasmid (GAL4AD) were moved into yeast cells carrying one of the four PthA variants fused to GAL4-BD domain as shown in the diagram (1 to 4, respectively). Yeast double-transformants were grown on SC-Trp-Leu-His-Ade in the presence of 5 mM of 3AT. None of prey fusions transactivated the reporter genes when co-transformed with empty bait vector (5). The PthA baits also did not transactivate the reporter genes when co-transformed with the empty prey vector in the same growth conditions (GAL4AD). (B) Western blot detection of eluted fractions from GST pulldown assays using the purified 6xHis-PthAs 1–4 as prey and immobilized GST or GST-fusion proteins as baits. Arrows indicate bands corresponding to the expected size for the GST-fusion proteins CsHMG (~45 kDa), CsTRAX (~55 kDa), CsSMC (~45 kDa), CsRRPMP1 (~50 kDa), CsRRMP2 (~46 kDa), CsPABP1 (~53 kDa) and CsVIP2 (~85 kDa) detected by the GST anti-serum. PthA proteins (~116–122 kDa) were detected using the anti-PthA serum. Recombinant PthAs 3 and 4 were added as references in the first lanes of the gels shown in the middle and right panels, respectively.
In the published figure, the third lane along shows CsMAF1 protein–as correctly denoted in the Plant Physiology article–but is incorrectly labelled as CsPABP1 protein in the PLOS ONE article. The authors would like to apologize for the error, which arose when the wrong lane was removed from the blot during preparation of the figure. The authors have repeated the GST-pulldown experiments with freshly prepared recombinant protein samples, and now provide a corrected Fig 1 that includes the blots obtained from the new experiments. Raw, uncropped blot images for both the new experiment and the original experiment are included as Supporting Information files. This correction does not affect the conclusions of the paper.
S1 File. Original experiment images: raw uncropped blots, SDS-PAGE gel, and Coomassie blue stained PVDF membrane.
S2 File. Repeated experiment images: raw uncropped blots and SDS-PAGE gel.
Citation: de Souza TA, Soprano AS, de Lira NPV, Quaresma AJC, Pauletti BA, Leme AFP, et al. (2015) Correction: The TAL Effector PthA4 Interacts with Nuclear Factors Involved in RNA-Dependent Processes Including a HMG Protein That Selectively Binds Poly(U) RNA. PLoS ONE 10(7): e0134818. https://doi.org/10.1371/journal.pone.0134818
Published: July 31, 2015
Copyright: © 2015 de Souza et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited