(a) A schematic showing the fabrication of a CNT/PCU composite thin film using spin casting techniques. (b) Transparency of PCU, 10% CNT/PCU, and 50% CNT/PCU. The CNT/PCU composites were made transparent under visible and optical microscopy. (c). Nanoscale surface topography of PCU, 10% CNT/PCU, and 50% CNT/PCU, as determined by AFM. An increase in the presence of nanostructures corresponded with increasing levels of CNTs embedded in PCU.
(a) The actin cytoskeleton (green) and focal adhesions (red) of pre-osteoblasts grown on the PCU (a, d), 10% CNT/PCU (b, e), and 50% CNT/PCU (c, f) surfaces after incubation for 4 hrs. (g-i) Relative mRNA expression levels of fold change of the integrin subunits α1, α2, α5, αv, β1, and β3. Pre-osteoblasts were grown on the pure PCU (orange) surface and the two CNT/PCU composite surfaces (gray for 10% CNT and dark gray for 50% of CNT in PCU). mRNA expression levels were determined using qPCR assays after 4-h culture. (j-k) Fold change of FBS and VN adsorption. (l) Fold change of pre-osteoblast cell adhesion levels on the CNT/PCU surfaces compared with that on the PCU surface after 4 hrs. All data represent the mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 vs. control (PCU) and ###p < 0.001 vs. CNT/PCU composites.
The mRNA levels of (a) Sp7 and Runx2 at 7 days and (b) Ibsp, Alpl, Col1, Spp1, and Bglap after 14 days in osteoblasts grown on the PCU (orange) and CNT/PCU composite surfaces (gray for 10% CNT and dark gray for 50% of CNT in PCU) were determined by qPCR. (c) Dominant biomarkers of osteoblast responses (short and long term). All data represent the mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 vs. control (PCU).
Citation: Lee JW, Park J-W, Khang D (2015) Correction: Analysis of Osteoblast Differentiation on Polymer Thin Films Embedded with Carbon Nanotubes. PLoS ONE 10(7): e0132874. https://doi.org/10.1371/journal.pone.0132874
Published: July 13, 2015
Copyright: © 2015 Lee et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited