Pseudomonas aeruginosa is a Gram-negative, metabolically versatile opportunistic pathogen that elaborates a multitude of virulence factors, and is extraordinarily resistant to a gamut of clinically significant antibiotics. This ability, in part, is mediated by two-component regulatory systems (TCS) that play a crucial role in modulating virulence mechanisms and metabolism. MifS (PA5512) and MifR (PA5511) form one such TCS implicated in biofilm formation. MifS is a sensor kinase whereas MifR belongs to the NtrC superfamily of transcriptional regulators that interact with RpoN (σ54). In this study we demonstrate that the mifS and mifR genes form a two-gene operon. The close proximity of mifSR operon to poxB (PA5514) encoding a ß-lactamase hinted at the role of MifSR TCS in regulating antibiotic resistance. To better understand this TCS, clean in-frame deletions were made in P. aeruginosa PAO1 creating PAO∆mifS, PAO∆mifR and PAO∆mifSR. The loss of mifSR had no effect on the antibiotic resistance profile. Phenotypic microarray (BioLOG) analyses of PAO∆mifS and PAO∆mifR revealed that these mutants were unable to utilize C5-dicarboxylate α-ketoglutarate (α-KG), a key tricarboxylic acid cycle intermediate. This finding was confirmed using growth analyses, and the defect can be rescued by mifR or mifSR expressed in trans. These mifSR mutants were able to utilize all the other TCA cycle intermediates (citrate, succinate, fumarate, oxaloacetate or malate) and sugars (glucose or sucrose) except α-KG as the sole carbon source. We confirmed that the mifSR mutants have functional dehydrogenase complex suggesting a possible defect in α-KG transport. The inability of the mutants to utilize α-KG was rescued by expressing PA5530, encoding C5-dicarboxylate transporter, under a regulatable promoter. In addition, we demonstrate that besides MifSR and PA5530, α-KG utilization requires functional RpoN. These data clearly suggests that P. aeruginosa MifSR TCS is involved in sensing α-KG and regulating its transport and subsequent metabolism.
Citation: Tatke G, Kumari H, Silva-Herzog E, Ramirez L, Mathee K (2015) Pseudomonas aeruginosa MifS-MifR Two-Component System Is Specific for α-Ketoglutarate Utilization. PLoS ONE 10(6): e0129629. https://doi.org/10.1371/journal.pone.0129629
Academic Editor: Min Wu, University of North Dakota, UNITED STATES
Received: February 24, 2015; Accepted: May 10, 2015; Published: June 26, 2015
Copyright: © 2015 Tatke et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Data Availability: All relevant data are within the paper.
Funding: This study was supported in part by the National Institutes of Health – Minority Biomedical Research Support SCORE (SC1AI081376; to KM), National Science Foundation IIP-1237818 [PFI-AIR: CREST-I/UCRC-Industry Ecosystem to Pipeline Research] (to KM), Biomedical Research Initiative (BRI) student research award (NIH/NIGMS R25 GM061347; to GT), Florida International University (FIU) teaching assistantship (Biological Sciences; to GT), FIU research assistantship (Herbert Wertheim College of Medicine; to GT), MBRS-RISE fellowship (NIH/NIGMS R25 GM061347; to LR). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Pseudomonas aeruginosa is a metabolically versatile, Gram-negative opportunistic pathogen that is well known for its extensive spatio-temporal distribution . It is a dominant nosocomial pathogen capable of causing acute and chronic infections in immunocompromised and immunosuppressed patients [2,3]. In particular, patients with AIDS, severe burn wounds, cystic fibrosis (CF), chronic obstructive pulmonary disease (COPD), non-CF bronchiectasis and neutropenia are predisposed to P. aeruginosa infections [1,4–7]. P. aeruginosa chronic pulmonary infections are characterized by intensive bronchial neutrophilic inflammation resulting in respiratory failure [8,9], a major cause of fatality in CF patients . Moreover, P. aeruginosa is associated with keratitis  and chronic suppurative otitis media  leading to visual impairment and deafness [13,14]. P. aeruginosa possess numerous virulence factors, both cell-surface associated and secretory, which significantly contribute to its pathogenesis . Effective treatment of P. aeruginosa infections is impeded by its extraordinary intrinsic and acquired resistance to numerous clinically important antibiotics . Thus, antibiotic resistance and expression of multi-determinant virulence factors are two critical hallmarks in P. aeruginosa infections that make it an intimidating pathogen.
Successful infection and disease progression depends significantly on the ability of any pathogen to effectively utilize available nutrients that are essential for its growth and survival. P. aeruginosa is renowned for its extraordinary ability to utilize wide range of organic compounds such as carbohydrates, amino acids, fatty acids, mono- and polyalcohols, di- and tri-carboxylic acids as sources of carbon, nitrogen and energy . However, unlike other bacteria where glucose is the preferred carbon source [17,18], P. aeruginosa preferentially utilizes tricarboxylic acid (TCA) cycle intermediates [19,20], specifically, C4-dicarboxylates of the TCA cycle such as malate, fumarate and succinate [19–21].
The TCA cycle is an amphibolic pathway that serves two main purposes: energy-generation in aerobic organisms (catabolism), and the generation of intermediates to serve as biosynthetic precursors for fatty acid, amino acid and carbohydrate synthesis (anabolism) . The metabolic intermediates of the TCA cycle consist of a group of organic anions that include C4-dicarboxylates (succinate, fumarate, malate and oxaloacetate), C5-dicarboxylates (alpha-ketoglutarate (α-KG)) and C6-tricarboxylates (citrate, isocitrate) [23,24]. However, the role of TCA cycle intermediates is not restricted to energy metabolism or to serve as biosynthetic precursors. In the recent years, TCA cycle intermediates, in-particular, succinate and/or α-KG have gained significant importance as biological signaling molecules in variety of organisms including, bacteria , animals  and plants .
Sensing the available nutrients is a prerequisite for mobilizing the uptake systems. Bacterial two-component systems (TCSs), involving a membrane-bound histidine sensor kinase (HK) and a cytoplasmic response regulator (RR) play an integral part in bacteria’s ability to sense physiological cues. In response to stimuli, the sensor autophosporylates at a conserved histidine residue at the C-terminus, and subsequently the phosphate is transferred to an aspartate residue at the N-terminus of the RR [28–30]. TCSs in Bacillus subtilis, Corynebacterium glutamicum, Escherichia coli, Klebsiella pneumoniae, Rhizobium meliloti and Rhizobium leguminosarum have been shown to regulate extracellular C4-dicarboxylates and tricarboxylates transport [28,31–36]. Of these, DctB-DctD in R. meliloti is an extensively studied TCS, which in coordination with sigma factor RpoN(σ54) regulates the extracellular transport of C4-dicarboxylates succinate, fumarate and malate [37,38].
Three TCS protein pairs in P. aeruginosa namely, PA5165/PA5166 (DctB/DctD), PA5512/PA5511 (MifS/MifR) and PA1336/PA1335 have been identified to be homologous to the Rhizobium C4-dicarboxylate transport regulatory DctB/DctD . Amongst the three, very little is known of PA1336/PA1335. The PA5165/PA5166 (DctB/DctD) TCS has been demonstrated to regulate the transport of C4-dicarboxylates, succinate, fumarate and malate in coordination with the sigma factor RpoN (σ54) . The SK MifS (65.3 kDa) and RR MifR (49.6 kDa) share 51% and 69% sequence identity to the R. meliloti DctB and DctD, respectively . The RR MifR is involved in regulating the maturation stage of P. aeruginosa biofilm formation as mifR deficient mutants fail to form microcolonies . Later studies reported the interdependence of pyruvate fermentation and functional MifR in supporting microcolony formation . However, the mechanism by which MifR is activated in this process remains obscure and no relation with HK MifS has been established. Using clean in-frame deletion mutants of the mifS, mifR and mifSR genes we show that MifSR TCS regulates P. aeruginosa α-KG transport and requires functional RpoN.
mifS and mifR are a part of a two-gene operon
In eubacteria, the genes that encode a HK and its cognate RR are often linked and are co-transcribed . Our sequence analysis of P. aeruginosa PAO1 genome revealed that mifS (PA5512) and mifR (PA5511) are adjacent to each other, in the same orientation. The predicted translation start site of mifR ORF overlaps with mifS translation termination codon indicating that they are cotranscribed (Fig 1A and 1B). To determine if these two genes form an operon, cDNA across the intergenic regions spanning mifS and mifR was amplified using GDT_cotransF1-R1 and GDT_cotransF2-R2 primers (see Materials and Methods). As expected, 200 bp and 100 bp products were detected when using primers that span the overlapping region (Fig 1C, Lane 3 and Lane 4). These results confirm that mifS and mifR are a part of a two-gene operon. As controls, the mifSR genes were also amplified (Fig 1C, Lane 2).
In P. aeruginosa PAO1 the mifR (PA5511) ORF has a translation start codon (ATG) overlapping the mifS (PA5512) termination codon (TGA), denoted in red (B), suggesting that the mifS and mifR genes are physically linked. The cDNA amplification of the intergenic region spanning the mifS and mifR genes using GDT_cotrans F1-R1 and GDT_cotrans F2-R2 primers (Table 1) confirm that the two genes mifS and mifR are co-transcribed and form an operon (C).
Loss of mifS and mifR did not affect antibiotic resistance
To identify the role of MifSR TCS, clean in-frame deletion mutants of mifS, mifR and mifSR were constructed in the prototypic P. aeruginosa PAO1. Henceforth they will be referred to as PAO∆mifS, PAO∆mifR and PAO∆mifSR, respectively. For complementation studies, recombinant plasmids containing the entire mifR, mifS and mifSR genes were constructed. The complementing plasmids with the genes are called pMifS, pMifR and pMifSR. These plasmids were introduced into the respective mutant strains.
Previous studies in our lab postulated that the MifSR TCS system, found 81-bp upstream of the pox operon, may contribute to P. aeruginosa ß-lactam resistance  as the genes regulated by TCS tend to be co-located on the chromosome . However, MIC analyses using E-test and micro-dilution methods showed that the loss of these genes did not affect the antibiotic resistance profile when compared to the parent strain, P. aeruginosa PAO1(Data not shown). Further, qRT-PCR studies showed that deletion of mifS, mifR and mifSR had no effect on the expression of poxB compared to the parent PAO1 (Fig 2).
Expression of poxB (PA5514) was tested in mifSR mutants relative to PAO1. Data was normalized to expression in PAO1. Bars above or below the line represents up- and down-regulation, respectively and the bars indicate standard errors. The clpX gene (PA1802) was used as the housekeeping control. There was no statistically significant difference (p-value > 0.05) between the wild type PAO1 and mifSR mutant strains as determined by one-way ANOVA and student’s unpaired t test.
mifS, mifR and mifSR mutants failed to grow in the presence of α-KG
The PAO∆mifS, PAO∆mifR and PAO∆mifSR mutants exhibited no discernible phenotype compared to the parent PAO1 when tested for growth, swimming, swarming, twitching motility (LB media), pyocyanin production (LB & King’s A media), pyoverdine production (LB & King’s B Media), congo red binding assay (CR media) and antibiotic resistance (MH media) (Data not shown). Hence, a comparative phenotypic microarray analysis was performed with the wild-type PAO1, PAO∆mifR and PAO∆mifS mutants (BioLOG Inc.). Out of approximately 2000 metabolic and chemical sensitivity assays tested, PAO∆mifR exhibited four gain-of-function and 29 loss-of-function phenotypes whereas PAO∆mifS exhibited two gain-of-function and 23 loss-of-function phenotypes (Fig 3A). A single gain of function phenotype shared between PAO∆mifS and PAO∆mifR, was the ability to utilize L-methionine. When metabolism and chemical sensitivity were compared, the mutants appear more sensitive to various antibiotics (Fig 3B). However, none of these were reproducible in the lab in the MH media. The loss of mifS and mifR resulted in differential phenotype in the presence of six metabolites, amongst which, two were common to both mifS and mifR mutants (Fig 3B). The shared metabolic phenotypes involved the utilization of L-methionine and α-KG (Fig 3C). Compared to the parent PAO1, the mutants did not exhibit any growth increase when provided with L-methionine (Fig 4). This could be simply due to the difference in culture conditions and BioLOG proprietary media.
To identify the role of P. aeruginosa mifSR TCS, comparative phenotypic microarray of PAO∆mifS, PAO∆mifR mutants and wild-type PAO1 strain was performed at BioLOG Inc. (Hayward, CA, USA). Venn diagram of differentially regulated phenotypes of the mutants compared to their isogenic parent PAO1, showing gain of function or loss of function phenotypes (A). Phenotypic differences were further classified based on metabolic and chemical sensitivity properties (B). The phenotypes common to both mifS and mifR mutants are listed (C).
Growth curves of P. aeruginosa wild-type PAO1 and mifSR mutants in M9 minimal media supplemented with glucose (30 mM) and methionine (5 mM) as carbon and nitrogen source.
The inability to utilize α-KG by PAO∆mifS (Fig 5A) and PAO∆mifR (Fig 5B) in the BioLOG assay was reproduced in M9 minimal media supplemented with 30 mM α-KG (Fig 5C). In fact, all three mutant strains, PAO∆mifR, PAO∆mifS and PAO∆mifSR failed to grow in the presence of α-KG (Fig 5C). To rule out potential toxicity, the wild-type P. aeruginosa PAO1 and the mutants were cultured in M9 minimal media with varying concentrations of α-KG, ranging from 1 to 80 mM (Fig 6). The mutants exhibited no growth in the presence α-KG after 24 h at 37°C, whereas the wild-type PAO1 exhibited an increase in growth that was proportional to α-KG concentration (Fig 6B). All subsequent experiments were done with 30 mM α-KG. The growth defect exhibited by PAO∆mifS, PAO∆mifR and PAO∆mifSR could be restored to the wild-type levels by introducing mifR and mifSR genes into the respective mutants (Figs 5D and 7A).
The loss of mifS and mifR results in a growth deficient phenotype in the presence of α-KG as a sole carbon source, as depicted by BioLOG plate PM1, well D6 (A and B). Loss of growth phenotype was confirmed by growing PAO1, PAO∆mifS, PAO∆mifR and PAO∆mifSR mutants in M9 minimal media with α-KG (30 mM) for 18 to 24 h at 37°C (C). The growth defect was rescued by expressing mifR and mifSR genes (D) and the gene encoding the α-KG specific transporter PA5530 (E) in trans.
PAO1 and its isogenic mifSR mutants, PAOΔmifS, PAOΔmifR and PAOΔmifSR were grown in M9 minimal media with varying concentrations of α-KG (1 to 80 mM) as the sole carbon source. Growth was monitored by measuring absorbance at 600 nm (OD600) over a period of 24 h at 37°C. OD600 at 0 h (A) and 24 h (B) is plotted against α-KG concentration. Results shown are mean with standard deviation of three biological replicates. Statistically significant difference between the wild type and mutants as determined by one-way ANOVA with Bonferroni’s post-hoc test, ** p-value < 0.001.
mifSR mutants exhibit α-KG dependent growth defect
α-KG is a key TCA cycle intermediate (Fig 8) and plays an important role in regulating carbon and nitrogen metabolism . It has been previously shown that P. aeruginosa preferentially utilizes TCA cycle intermediates as a carbon source over other compounds [20,21,45]. To test if the growth defect exhibited by the loss of mifS and mifR is restricted to α-KG utilization, the mutants and the complementing strains were grown in the presence of TCA cycle intermediates citrate, succinate, fumarate, malate and oxaloacetate at 30 mM each. No difference in growth was observed between wild type PAO1 and its isogenic mutants in the presence of other TCA cycle intermediates except for α-KG (Table 1). This is not surprising as P. aeruginosa can use the glyoxylate shunt pathway to bypass the need for α-KG (Fig 8) . Furthermore, no difference in the growth profile of the wild type PAO1 and mifSR mutants was observed when grown in the presence of sugars, glucose and sucrose (30 mM each) (Data not shown). To reconfirm that the presence of α-KG is not toxic, the cells were grown in the presence of citrate and succinate combined in equal concentration with α-KG. The mutants and the wild type shared similar early exponential growth (Fig 9). However, the mutants reached stationary phase earlier as compared to the parent strain PAO1. This suggests that the presence of excess carbon source in the form of α-KG further contributes to the growth of PAO1. These analyses indicate that mifSR mutants are only defective in α-KG utilization.
Enzymes converting iso-citrate to α-KG (iso-citrate dehydrogenase: Icd, Idh), α-KG to succinyl-coA (α-KG dehydrogenase complex: SucA, SucB, Lpd3) and those involved in the glyoxylate shunt (isocitrate lyase (AceA) and malate synthase G (GlnB)) are shown in bold. Green boxes indicate the amino acid biosynthetic precursors of α-KG involved in the anaplerotic reaction.
To determine if α-KG is toxic to the cells, wild-type PAO1 and mifSR mutants were grown in the presence of α-KG in combination with succinate (A) and citrate (B) at 30 mM each. In comparison to the wild-type PAO1, mifSR mutants shared a similar exponential phase but reached stationary phase earlier, suggesting that it has depleted usable C-source. This suggests that PAO1 can efficiently utilize excess carbon source in the form of α-KG contributing to its increased growth.
mifSR mutants are defective in α-KG transport
The absence of growth in the presence of exogenous α-KG could be due to either failure to enter the cells or loss of the mutants’ ability to convert α-KG to succinate. The latter is likely if the mutants failed to express a functional α-KG dehydrogenase complex. The ability of mifSR mutants to grow effectively in the presence of citrate and succinate suggests that these mutants are likely to harbor a functional α-KG dehydrogenase complex, unless the mutants bypass it using the glyoxylate shunt (Fig 8). The former is likely as qPCR analysis of genes encoding isocitrate dehydrogenase (idh, icd) and α-KG dehydrogenase complex (sucA, sucB, lpd3) revealed no difference in the expression levels in the wild-type PAO1 and mifSR mutants (Fig 10).
RNA was isolated from cells grown in M9 minimal media supplemented with citrate (30 mM), reverse transcribed to cDNA and the presence of specific transcripts was analyzed by qPCR using gene-specific primers (Table 5). The expression of genes encoding aconitate hydratase 1 (acnA (PA1562)) isocitrate dehydrogenase (idh (PA2623)) isocitrate dehydrogenase, α-KG dehydrogenase complex (icd (PA2623)), sucA (PA1585) and lpd3 (PA4829), and σ54 (rpoN (PA4462)) were analyzed in mifSR mutants relative to PAO1 (log10 RQ = 1). Bars above or below the line represents up- and down-regulation, respectively and the bars are standard errors. The clpX (PA1802) gene was used as the housekeeping control. Statistically significant difference between the wild type and mutants as determined by one-way ANOVA with Bonferroni’s post-hoc test. Difference in the expression levels of genes is not statistically significant at p-value < 0.05.
α-KG is a hub for anaplerotic reactions, a process for replenishing TCA cycle intermediates. In this process glutamate, glutamine, proline and arginine act as precursor molecules for α-KG synthesis . Growth studies in the presence of these amino acids would serve as another indirect measure to test the functionality of α-KG dehydrogenase complex in mifSR mutants. To test this hypothesis, PAO1, PAO∆mifR, PAO∆mifS and PAO∆mifSR mutants were cultured in the presence of glutamate, glutamine, proline and arginine (Table 2). The parent PAO1 and the isogenic mutants exhibited similar growth phenotype. From the expression studies and growth analyses we deduce that the mifSR mutants are impaired in α-KG transport.
mifSR TCS genes regulate extracellular α-KG transport
In a recent study using transposon mutagenesis; PA5530 was identified as the functional α-KG transporter . To confirm the role of P. aeruginosa PA5530 in α-KG uptake and identify the role of mifSR genes, the gene was amplified and subcloned downstream of the inducible PlacUV5 promoter. The plasmid pPA5530 was introduced into PAO1 and the mifSR mutants. Expression of PA5530 in trans in PAO∆mifS, PAO∆mifR, PAO∆mifSR mutants restored their growth to a level similar to the wild-type PAO1 in M9 minimal media with α-KG (30 mM) as the sole carbon source (Fig 7B). Expression of an extra copy of PA5530 gene in the wild-type PAO1 did not affect its growth (Fig 5E). This finding suggests that expression of PA5530 is likely regulated by MifSR and/or α-KG. In fact, expression of PA5530 is regulated by α-KG, as seen in qRT-PCR analysis when PAO1 was grown in M9 media with varying amounts α-KG (Fig 11A). The loss of mifS, mifR and mifSR results in a significant decrease in PA5530 expression as compared to the wild type PAO1 in the presence of α-KG (Fig 11B). Thus, α-KG-dependent PA5530 expression requires MifS and MifR.
PA5530 gene expression was determined in the wild type PAO1 with varying concentrations of α-KG (1 h) (A). In addition, the expression of PA5530 was tested in mifSR mutants relative to PAO1, with cells exposed to 30 mM α-KG for 1 h (B). Data was normalized to expression in PAO1 under the respective conditions. Bars above or below the line represents up- and down-regulation, respectively and the bars indicate standard errors. The clpX gene (PA1802) was used as the housekeeping control. Statistically significant difference between the wild type and mutants as determined by one-way ANOVA with Bonferroni’s post-hoc test, ** p-value < 0.001.
RpoN (σ54) is required for α-KG utilization
The closest P. aeruginosa MifS and MifR homologs are R. meliloti DctB and DctD . In fact, MifR is 69% similar to R. meliloti DctD that belongs to the Sigma 54 (σ54) dependent NtrC family of transcriptional regulators [39,40]. Thus, it is likely that MifR has the conserved domains found among NtrC family of regulators, an N-terminal regulatory, a central σ54 activation and a C-terminal DNA binding domains [49,50]. MifR analysis using the simple modular architecture research tool (SMART)  and InterPro  revealed the presence of three domains: CheY-homologous receiver/regulatory, a central AAA+ region required for σ54 activation, and the DNA binding helix-turn-helix domains (Fig 12A). The central AAA+ domain contains seven conserved regions designated C1 to C7  that are characteristic of σ54- dependent transcriptional regulators. Sequence analysis of MifR revealed the presence of all the seven conserved regions in the AAA+ domain between amino acid residues 144 to 373 (Fig 12B).
(A) MifR domain organization determined using the Simple Modular Architecture Research Tool (SMART) . MifR is a sigma-54 dependent transcriptional activator . There are three functional domains, N-terminal receiver with the conserved aspartate residue at position 53 (Asp-53) (Purple), central AAA+ ATPase, characteristic of sigma-54 dependent activation proteins (Green), and the C-terminal helix-turn-helix (HTH) DNA binding (Red) domains. (B) Sequence alignment of MifR with P. aeruginosa DctD (PA5166), NtrC (PA5125) and R. meliloti DctD. Vertical bars indicate conserved residues, asterisk (*) indicate residues are identical at that position. Key residues of the central AAA+ domain (C1 to C7) are well conserved amongst sigma-54 dependent transcriptional activators. The horizontal arrow bars indicate HTH domain. Asp-53 indicates the conserved phosphorylation site of P. aeruginosa MifR. The alignment was generated using ClustalW2 (http://www.ebi.ac.uk/Tools/msa/clustalw2/).
Since MifR exhibits high identity to σ54-dependent transcriptional regulators, we hypothesized that P. aeruginosa rpoN mutants should exhibit a α-KG-dependent phenotype, similar to the mifSR mutants. To verify this hypothesis, we tested the ability of PAO∆rpoN mutant to grow in the presence of α-KG (30 mM) (Table 3). As expected, PAO∆rpoN failed to grow in the presence of α-KG (Table 3). The growth of the rpoN mutant was restored in PAO∆rpoN::rpoN complementing strain. Further, in trans expression of mifR and mifSR in PAO∆rpoN mutant failed to restore their growth in the presence of α-KG (Table 3). This data confirms that MifR regulatory function requires functional RpoN (σ54).
The small 81-bp mifSR promoter has no obvious RpoN sigma factor -12/-24 consensus sequence: 5’-TGGCACG-N4-TTGCW-3’ in which W stands for either A or T (Fig 13A) . In fact, it appears to have a potential -10 (consensus: TATAAT) but lacked -35 (consensus: TTGACA) for sigma-70 promoter (Fig 13A) . On the other hand, the promoter region of PA5530 is 315-bp long with strong -12 and -24 boxes upstream of the predicted transcription start site (Fig 13B). We hypothesized that the inability of rpoN mutant to utilize α-KG can be rescued by expressing PA5530 under a regulatable promoter PlacUV5. As expected, the growth of the rpoN mutant was restored when the plasmid harboring the transporter PA5530 was expressed in trans (Table 3). This suggests that expression of PA5530 requires both MifSR TCS and RpoN.
Motif search was done using the ensemble learning method SCOPE and GLAM2 (Gapped Local Alignment of Motifs) [113,114]. (A) Sequence analysis of the 81-bp (PmifS) (black) indicates a putative σ70-dependent -10 consensus (TATAAT). However, it lacks the -35 consensus (TTGACA) for σ70 promoter . Arrows represent the long 17-bp direct and inverted repeats in PmifS with a consensus GGAt/cAGCGACATCGGCG. (B) The 315-bp promoter region of PA5530 showing strong -12 and -24 σ54-dependent promoter like element and the proposed transcription start site (+1). Dashed line (blue) depicts a common motif in PmifS and PPA5530 suggesting a common regulatory mechanism (A and B). The three pairs of direct repeats in PPA5530 are represented by green, blue and orange arrows. PPA5530 possess the signature sequence (AAc/uAAc/uAA) for catabolite repression control (Crc) protein (brown box) . The uncharacterized small antisense RNA (asRNA) identified in the PPA5530 region  is indicated by marked line.
The presence of a common motif, GATCGGCGGATt/gTCC, in the PmifS and PPA5530 (Fig 13A and 13B) suggest that these two operons share some common regulatory mechanism. In addition, both promoters possess multiple motifs: PmifS has two sets of large overlapping inverted repeats, and PPA5530 has three sets of direct repeats (Fig 13A and 13B). However, the role of these motifs remains to be elucidated.
P. aeruginosa pathogenicity relies significantly on its metabolic flexibility. However, establishment of successful infection and its progression requires more than just meeting nutritional demands. Precision in sensing environmental signals concomitant with a quick and appropriate response is the key to efficient bacterial adaptation and survival. An arsenal of TCSs encoded in its genome has furnished P. aeruginosa with a sophisticated capability to regulate diverse metabolic and virulence processes, ensuring its success as a pathogen [55–57]. P. aeruginosa genome encodes one of the largest groups of TCS proteins identified in any sequenced bacterial species [57,58]. Bacterial TCS’s sense and respond to a variety of external cues such as nutrient availability, osmolarity, redox state, temperature, and concentrations of other extracellular molecules . However, very few TCS signaling molecules have been identified to date. In this study we suggest that the P. aeruginosa MifSR TCS exclusively senses α-KG, a C5 dicarboxylate and a key component of TCA cycle.
P. aeruginosa antibiotic resistance is independent of MifSR TCS
A common feature of bacterial genomes is a close association between the functionally related genes and their location on the chromosome [60,61]. Typically, genes encoding functionally related HKs and RRs are often physically linked and are co-transcribed as an operon [30,62]. Indeed, our in silico analysis (Fig 1A and 1B) and cDNA amplification (Fig 1C) reveled that mifS-mifR genes are co-transcribed and form an operon. This also suggests that HK-MifS and RR-MifR are functionally related and work as a TCS pair. In addition, TCS proteins are known to regulate expression of genes in their immediate vicinity . The mifSR genes are 81 bp upstream of the two-gene poxAB (PA5513-5514) operon. Due to the proximity of mifSR to poxB which encodes for a β-lactamase, we postulated that mifSR TCS regulates antibiotic resistance. However, our initial results nullified this hypothesis in which comparative MIC’s (Data not shown) and qRT-PCR data (Fig 2) showed no difference in antibiotic resistance profiles or poxB expression between the wild-type PAO1 and mifSR single and double deletion mutants.
MifSR TCS regulates P. aeruginiosa α-KG utilization
A previous transcriptome study of the wild-type PAO1 and a mifR deletion mutant cultivated under biofilm-specific conditions showed significant alteration in the expression of genes involved in regulating P. aeruginosa metabolism, small molecule transport and amino acid biosynthesis . The majority of the changes observed in phenotypic microarrays of the mifS and mifR mutant strains cultivated under planktonic conditions were associated with chemical sensitivity and not with metabolism (Fig 3B). Only 12–16% of phenotypic changes were associated with metabolism. This confirms the significant metabolic differences in the rich planktonic versus anaerobic mode of biofilm growth in P. aeruginosa .
Petrova et al. (2012) have also demonstrated that genes involved in energy metabolism, including anaerobic metabolism and fermentative pathways using arginine (arcDABC) and pyruvate, were expressed significantly less in ∆mifR mutant biofilms as compared to its parent PAO1 . Though pyruvate is needed for biofilm formation, it cannot compensate for the loss of mifR . Interestingly, the biofilm phenotype associated with the loss of mifR can be complemented by ldhA encoding D-lactate dehydrogenase to wild type levels of biomass accumulation and microcolony formation . These findings suggest that MifR somehow regulates expression of ldhA, a second gene in a three-gene operon gacS-ldhA-PA0926 . Importantly, analyses of the promoters reveal the presence of a shared motif in PmifS (GATCCGCCGATGTCC) and PPA5530 (GATCGGCGGATTTCC) (Fig 13) and PgacS (AATCCGCCGGGCTGC) suggesting a possible coordinate regulation, and that need to be verified.
Our phenotypic microarray analyses and growth experiments suggested that P. aeruginosa α-KG utilization requires MifS and MifR (Figs 5 and 7A). The ability of PAOΔmifR, PAOΔmifS and PAOΔmifSR to grow in the presence of α-KG was restored by in trans expression of mifR and mifSR (Fig 7A). Interestingly, the PAO∆mifS was complemented by pMifR and pMifSR (Fig 5D) but not by pMifS alone. To rule out that gene expression may have been compromised, the mifS gene was cloned downstream of the inducible PlacUV5 promoter. Though the expression of stable protein was visible in a protein gel, it failed to complement PAO∆mifS mutant (data not shown). This suggests that cis-expression of mifS and mifR is critical for MifS-function. Other researchers have encountered similar problems involving histidine kinases . Moreover, complementation of the PAO∆mifS with pMifR suggests that either phosphorylation is not required or there is a potential crosstalk between MifR and other non-cognate HKs. Alternatively, phosphorylation of MifR can occur through small molecule phosphor-donors, like acetyl phosphate, carbamoyl phosphate and phosphoramidate . Such phenomenon is observed with other TCS RRs [66–68]. However, this has to be verified.
The C5-dicarboxylate α-KG is an important intermediate in the energy-generating TCA cycle (Fig 8) and plays a key role in regulating carbon and nitrogen metabolism . Similar to other bacteria , TCS’s in P. aeruginosa have been reported to regulate transport and utilization of TCA cycle intermediates such as succinate, fumarate, malate and citrate [39,56]. The R. meliloti DctB/DctD system is a well-characterized TCS that controls the transport of TCA cycle C4-dicarboxylates succinate, fumarate and malate . Though P. aeruginosa MifS/MifR proteins are homologous to R. meliloti DctB/DctD TCS proteins, the mifSR mutants efficiently utilized citrate, succinate, fumarate, malate, oxaloacetate, sucrose and glucose but exclusively failed to grow in the presence of α-KG (Table 1). This was further supported by another parallel study that shows that α-KG utilization requires MifR . Thus, the P. aeruginosa MifSR TCS is specifically and uniquely involved in C5-dicarboxylate α-KG utilization.
MifSR TCS modulates P. aeruginosa α-KG transport
The inability to utilize α-KG suggested that the mifSR mutants either have a defective α-KG dehydrogenase complex (inability to convert α-KG to succinyl-coA, Fig 8), or they are deficient in the transport of α-KG into the cell. The former was ruled based upon multiple findings: unchanged expression levels of genes encoding α-KG dehydrogenase, lpd3 (PA4829) and sucA (PA1585) (Fig 10); the ability to use C4 and C6 dicarboxylates (Table 1) and C5 family of amino acids such as arginine, proline, glutamine, and histidine (Table 2). The C5 family of amino acids act as biosynthetic precursors of glutamate that ultimately are converted to α-KG by a transamination reaction or through the action of glutamate dehydrogenase . These findings strongly argued that the mifSR mutants were defective in their ability to transport α-KG into the cell.
To date, among the identified carboxylate transporters, the C4-dicarboxylate transporters have been reasonably well characterized. Based on protein sequence similarity analysis, bacterial C4-dicarboxylate transporters are classified into five families, namely, dicarboxylate transport (DctA); dicarboxylate uptake (DcuAB), (DcuC) and (CitT) and the tripartite ATP-independent periplasmic (TRAP) families . Amongst these, DctA transporters, a subgroup of the dicarboxylate/amino acid:cation symporter (DAACS) family [71–73], are extensively studied and are implicated in the transport of C4-dicarboxylates in Echerischia coli , Bacillus subtilis , Rhizobium meliloti [38,75], Rhizobium leguminosarum [37,76] and Corynebacterium glutamicum . As we were trying to identify the MifSR-dependent transporter Lundgren et al., reported that PA5530 is involved in α-KG transport . As predicted, in trans expression of PA5530 was able to restore the ability of mifR, mifS and mifSR mutants to grow in α-KG (Fig 5E). This is further confirmed by the increase in PA5530 expression in PAO1 in the presence of α-KG (Fig 11A). PA5530 shares no homology with the P. aeruginosa C4-dicarboxylate transporter PA1183 (DctA). However, it does have conserved protein domain family PRK10406 implicated in α-KG transport and shares ~70% homology to E. coli and Erwinia spp. α-KG permease KgtP [78,79]. A common feature in the transport of C4-dicarboxylates and other carbon sources in different bacteria is the involvement of TCS mediated regulatory mechanism. Involvement of TCSs, a stimulus-response coupled mechanism, in the transport of C5-dicarboxylates suggests a more profound role of α-KG as a signaling molecule.
P. aeruginosa α-KG transport requires functional RpoN (σ54)
P. aeruginosa RpoN (σ54) is involved in a myriad of functions including expression of virulence factors and nutrient uptake . Functional RpoN is reported to be critical for maintaining a carbon-nitrogen balance in Pseudomonads [56,81–84]. Sequence analysis of MifR indicated a requirement of functional RpoN in modulating P. aeruginosa α-KG utilization. Our study confirms that α-KG utilization in P. aeruginosa PAO1 requires functional RpoN (Table 3). This phenotype is not strain-specific as phenotypic microarray profiling (BioLOG) of P. aeruginosa PA14 rpoN mutant exhibited a similar phenotype, a significant difference in the ability to utilize α-KG as a carbon source as compared to the wild-type PA14 . An RpoN-dependent phenotype was also observed with citrate and 4-hydroxyphenylacetate utilization . Similarly, utilization of C4-dicarboxylates succinate, fumarate and malate in R. meliloti and P. aeruginosa also requires the sigma factor RpoN (σ54) [37,39,86].
The need for RpoN (σ54) to utilize α-KG in P. aeruginosa can be bypassed by expressing PA5530 encoding for the transporter under a regulatable promoter but not MifS and MifR. Consistent with the need for RpoN (σ54), the promoter for PA5530 has the requisite signature sequences (Fig 13). Like most complex RpoN-dependent promoters , the region is long with multiple motifs that include a signature sequence (AAc/uAAc/uAA) for catabolite repression control (Crc) protein, a post-transcriptional inhibitor that binds the mRNA preventing translation [88–90]. Expression of crc is in-turn regulated by RpoN-dependent non-coding RNA CrcZ  whose absence in rpoN mutant can also lead to reduced expression of PA5530. Also, analysis of P. aeruginosa PA14 transcripts indicates that the PA5530 promotor is under a small non-coding antisense RNA (asRNA) regulation . Though the role of Crc, CrcZ and the asRNA in α-KG transport has to be verified experimentally, it suggests an additional layer of regulation superimposed on the need for MifS and MifR on the expression of the C5-dicarboxylate transporter PA5530.
In eukaryotic cells, the mitochondria serve as a hub and reservoir of the TCA cycle and its intermediates, respectively. Bacterial pathogens can be highly virulent intruders of the host tissue, causing significant damage leading to cellular aberrations and injury. Mitochondrial dysfunction, a consequence of cell injury, results in efflux of TCA cycle intermediates leading to an increase in their extracellular concentrations . It is known that TCA cycle intermediates (C4, C5, and C6 dicarboxylates) are present at micromolar (μM) concentrations in blood that increase with tissues damaged [26,92]. α-KG can also act as a reactive oxygen species scavenger, especially for hydrogen peroxide, protecting both host and pathogen . For pathogenic bacteria such as P. aeruginosa, efficient uptake of TCA intermediates from the host is crucial for its survival, especially when it is bombarded with host reactive oxygen species, and requires the activity of bacterial carboxylate transport proteins. The transport proteins could be specific for C4, C5, and C6 intermediates and may use a cognate TCS. This study suggests a complex regulatory cascade in modulating P. aeruginosa C5-dicarboxylate, α-KG uptake involving the PA5530 transporter, the MifS/MifR TCS and the sigma factor RpoN (Fig 14). It appears that MifS senses the presence of α-KG and signals MifR. The activated MifR in concert with RpoN initiates the transcription of α-KG-specific transporter gene PA5530. Analyses of the published data suggests that the PA5530 promoter is under several layers of regulation including catabolite repression mediated by Crc/CrcZ  and the small non-coding asRNA . Though the asRNA has been identified , it has not been characterized. It is not surprising that the PA5530 expression is potentially regulated by Crc, as it would allow control of transporter(s) in response to the presence of carbon sources in the environment.
HK-MifS senses the extracellular α-KG to undergo phosphorylation. The phosphate is transferred to the RR-MifR. The phosphorylated MifR in coordination with RpoN (σ54) activates the expression of α-KG specific transporter gene PA5530. PA5530 thus enables the influx of α-KG to meet the metabolic and energy demands of the cells. PA5530 promoter (PPA5530) region has a Crc binding site (Fig 13), suggesting that it is under the catabolite repression control by Crc/CrcZ. The PPA5530 also shows the presence of another uncharacterized small non-coding asRNA indicating a multilayered and complex regulation of the α-KG transport system.
In addition to MifSR (PA5512/PA5511), PA1336/PA1335 have been identified to be homologous to the Rhizobium C4-dicarboxylate transport regulatory DctB/DctD TCS [39,40]. However, the role of PA1336/PA1335 remains to be elucidated. The P. aeruginosa genome also encodes 19 other paralogs of PA5530 dicarboyxlate transporters, most of which have share less than 50% similarity except for PA0229 (PcaT). PA0229 and PA5530 have 73% similarity. Future studies will determine if the transporters are preferentially or hierarchically upregulated depending on the carbon source. It is also important to note that much of bacterial physiology, particularly of pathogens such as P. aeruginosa remains a mystery. Metabolic versatility, expression of virulence factors and antibiotic resistance together makes P. aeruginosa an portentous pathogen. Thus, understanding the physiological cues and regulation would provide a better stratagem to fight the often indomitable infections.
Materials and Methods
Strains, media and growth conditions
P. aeruginosa wild-type PAO1  and its derivatives PAO∆mifS, PAO∆mifR, PAO∆mifSR and PAO∆rpoN or Escherichia coli strain DH5α were used in this study (Table 4). Saccharomyces cerevisiae strain InvSC1 (Invitrogen, Life Technologies, Carlsbard, CA, USA) was used for in vivo homologous recombination . Briefly, all bacterial cultures were grown in Luria Bertani (LB) broth (5 g tryptone, 10 g sodium chloride, and 5 g yeast extract per liter) or agar (LB broth with 1.5% agar) (Difco, NJ, USA) or M9 minimal Media (64 g Na2HPO4-7H2O, 15 g KH2PO4, 2.5 g NaCl, 5.0 g NH4Cl, 20 mM MgSO4, 1 mM CaCl2 per liter)  at 37°C, unless specified otherwise. Yeast extract-peptone-dextrose media (YEPD: 20 g Bacto Peptone, 10 g yeast extract, 20 g dextrose per liter) was routinely used to culture S. cerevisiae and synthetic define agar-uracil media was used as selection media for pMQ30 yeast transformants . P. aeruginosa competent cells were prepared as previously described . For growth curve and complementation studies M9 minimal media supplemented with glucose, sucrose or TCA cycle intermediates including citrate, α-KG, succinate, fumarate, malate or oxaloacetate were used as a sole carbon source at 30 mM each unless specified otherwise. Motility assays were performed in LB media (Difco, NJ, USA). For pyocyanin and proverdine production strains were cultivated in King’s A medium (Difco, NJ, USA) and King’s B medium . Cation-adjusted Mueller Hinton broth and agar (Difco, NJ, USA) was used in MIC assays. For plasmid maintenance, antibiotics were added to growth media when appropriate, at the specified concentrations: E. coli: ampicillin (Ap) 100 μg/ml, gentamycin (Gm) 15 μg/ml, kanamycin (Km) 20 μg/ml, P. aeruginosa: Gm 75 μg/ml.
Genetic manipulations were carried out using standard techniques . Primers were synthesized by Integrated DNA Technologies, Inc. (Coralville, IA, USA) and are listed in Table 5. Plasmid DNA isolation was carried out using PureLink Hipure Plasmid Miniprep Kit (Invitrogen, Life Technologies, Carlsbard, CA, USA) and agarose gel fragments were purified using Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA). RNA and cDNA was made using RNeasy Mini Kit (Qiagen Inc. Venio, Limburg, Netherlands) and SuperScript III First-Strand Synthesis System (Invitrogen, Life Technologies, Carlsbard, CA, USA). Restriction endonucleases were from New England Biolabs (Ipswich, MA, USA) and DNA sequencing was carried out at Florida International University (FIU) DNA core and at GENEWIZ Inc (South Plainfield, NJ, USA). All other chemicals were purchased from SIGMA-ALDRICH (St. Louis, MO, USA), AMRESCO (Solon, OH, USA) and Fisher Scientific (Waltham, MA, USA).
Construction of P. aeruginosa ∆mifR mutant
An unmarked mifR clean in-frame deletion mutant of P. aeruginosa was generated by gene splicing . Upstream and downstream flanking regions of mifR were amplified by PCR (GC Rich PCR System, Roche, Indianapolis, IN, USA), using primers listed in Table 5. A 754-bp P1 and a 720-bp P2 were amplified using upstream primers mifRUF1-EcoRI and mifRUR1-NheI and the downstream primers mifRDF1-NheI and mifRDR1-HindIII (Table 5), respectively from PAO1 genomic DNA. After sequencing to ensure fidelity, P1 and P2 were spliced together to obtain a 1474-bp deletion fragment with a deletion of mifR containing stop codons at its junction (inserted as part of NheI site in the primer). This was then sequenced and subcloned into a P. aeruginosa non-replicative plasmid pEXG2  as a EcoRI-HindIII fragment and moved into the wild-type PAO1 strain by allelic replacement  using pRK600 and pRK2013 as the helper plasmids [100,101]. Clones were screened for Gm sensitivity (75 μg ml−1) and sucrose resistance (8% sucrose) corresponding to a double cross-over recombination event and replacement of the target gene with the deletion product. The presence of the deletion in PAOΔmifR (PKM901) was confirmed by PCR amplification and sequencing of the deletion product (data not shown).
Construction of P. aeruginosa mifS and mifSR mutants
The unmarked mifS and mifSR deletion in PAO1 was generated by using the yeast system of double-stranded gap repair and homologous recombination . Briefly, the mifS and mifSR upstream and downstream flanking regions were amplified by PCR using primers listed in Table 5. To create a mifSR deletion, an upstream 933-bp P1 and a downstream 1115-bp P2 were amplified using primer pairs mifSRUF1-mifSRDF1 and mifSRUR1-mifSRDR1, respectively. Similarly, to create mifS deletion, an upstream 703-bp P1 and a downstream 653-bp were amplified using primer pairs HKmifSUF-HKmifSDF and HKmifSUR-HKmifSDR, respectively. HKmifSUF and mifSRUF1 primers had stretches of homologous DNA, 5’-GGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCT-3’ and 5’-CCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGAT-3’, respectively, to target recombination of the amplicons with pMQ30 vector. These primer pairs also had complementing sequences at the 3’ end to facilitate joining to create the P3 fragment, as well as stop codons (CTAGTTAGCTAG) to prevent any run off translation. The pMQ30 vector has double selection markers URA3 for yeast and gentamycin for E. coli . Yeast cells were transformed with the P1, P2 and linearized pMQ30 (BamHI digested) using standard protocols  and colonies were selected on sucrose-uracil plates.
The yeast colonies were checked for the presence of P3 constructs for mifS and mifSR deletions by amplification using upstream forward (mifSRUF1 and HKmifSUF, respectively) and downstream reverse (mifSRDR1 and HKmifSDR, respectively) primers. Yeast DNA was isolated from the positive colonies as described earlier . E. coli was transformed with the recombinant pMQ30 plasmids containing P3s and screened for gentamycin resistance. The amplified P3s from the recombinant plasmids were sequenced to ensure fidelity. The constructs were then moved into PAO1 strain using tri-parental mating and screened for single and double crossovers using counter selection with sucrose and gentamycin as described earlier [107,108]. The presence of the gene deletions in all the mutants were confirmed using standard molecular methods (PCR and DNA sequencing of the locus). These strains are henceforth referred to as PAOΔmifS (PKM900) and PAOΔmifSR (PKM902).
Construction of complementing plasmids
DNA fragments from P. aeruginosa PAO1 with mifS (~1.77 kb) and mifR (~1.35 kb) were PCR amplified using primer pairs HK_mifSF1-HK_mifSF1R1, GDT_mifRF1-GDT_mifRR1, respectively. In order to ensure expression of the genes, the primers are designed such that the ORF will juxtapose against a strong ribosome binding site . The PCR amplified products were cloned into pCR2.1 TOPO (Invitrogen, Life Technologies, Carlsbard, CA, USA) using manufacturers protocol to generate plasmids pGDT001 and pGDT002, respectively. The fidelity of the PCR amplified product was confirmed by sequencing. The fragments carrying mifS and mifR were moved into a broad host range pPSV37-Gm plasmid  as a NheI-SacI fragments, downstream of an inducible PlacUV5 promoter to generate plasmids pGDT003 and pGDT004, respectively. Henceforth, these plasmids are referred to as pMifS and pMifR.
DNA fragments from PAO1 with mifSR (~3.12 kb) and PA5530 (~1.3 kb) were PCR amplified using primer pairs HK_mifSF1-GDT_mifRR1 and GDT_PA5530F1-GDT_PA5530R1 (Table 5), respectively. The PCR amplified products were cloned directly into pPSV37-Gm plasmid as NheI-SacI fragments, downstream of an inducible PlacUV5 promoter to generate plasmids pGDT005 and pGDT006, respectively. Sequence fidelity was confirmed by sequencing using the primers GDT_p37_SeqF-R, mifR_seqF-R, mifS_seqF-F2 and PA5530_seqF-R (Table 5). Henceforth, these plasmids are referred to as pMifSR and pPA5530.
These expression plasmids were then introduced into wild-type PAO1, PAO∆mifS, PAO∆mifR, PAO∆mifSR and PAO∆rpoN deletion mutants by electroporation  and gentamycin resistant colonies were selected.
Comparative phenotypic microarray profiles of wild-type PAO1 with PAO∆mifR and PAO∆mifS mutant were performed at BioLOG Inc. (Hayward, CA, USA). Phenotypic profiling was carried out in triplicate and data analyses was done using OmniLog PM software.
P. aeruginosa PAO1 and its derivatives were grown overnight at 37°C in LB broth with or without antibiotics. Overnight cultures were washed with sterile 0.85% NaCl (wt/vol) solution to remove spent and residual media. Cultures were diluted in fresh M9 minimal media to obtain equal optical densities (OD600) of 0.025. Growth of the cultures was assessed in LB broth and in M9 minimal media supplemented with glucose (30 mM), sucrose (30 mM) or TCA cycle intermediates including citrate, α-KG, succinate, fumarate, malate or oxaloacetate (at 30 mM, unless specified otherwise) as a sole carbon source in 48 and 96 well plates (Falcon). Growth was monitored by determining absorbance at 600 nm using BioTek Synergy HT (Winooski, VT, USA) plate reader for 18–24 h at 37°C. All experiments were performed multiple times in triplicate.
Pyocyanin and pyoverdine production
Extracellular pyocyanin was quantified by extracting the pigment from culture supernatants using the chloroform-HCL method as described previously . Briefly, 5 ml culture supernatants from stationary-phase cultures (∼18 h) grown in King’s A medium was extracted with 3 ml chloroform. Pyocyanin was then re-extracted into 1 ml of 0.2 N HCl, resulting in a pink color, indicating the presence of pyocyanin that was read at 520 nm. The concentration is expressed as μg of pyocyanin produced per ml of culture (μg/ml), by multiplying the optical density OD520 by 17.072 .
To measure pyoverdine production, cells were grown overnight at 37°C in King’s B medium . Pyoverdine in the supernatant was read at 405 nm and normalized to the initial cell density (OD600). Pyoverdine levels were expressed as a ratio of OD405/OD600 .
Minimum Inhibitory Concentration
MICs were determined using the E-test as per the manufacturers protocol (BioMerieux, USA) and/or by standard broth microdilution method . The assays were performed in triplicate, each with technical triplicate, for each antibiotic in cation-adjusted Mueller Hinton broth.
RNA isolation, cDNA synthesis and qRT-PCR
RNA was isolated from P. aeruginosa wild-type PAO1, PAO∆mifR, PAO∆mifS and PAO∆mifSR strains grown in LB broth followed by 1 h treatment with 30 mM α-KG. Briefly, overnight cultures grown in LB broth at 37°C were washed with sterile 0.85% saline solution to remove spent media and were subcultured at 37°C, 200 rpm in LB media. LB broth was used as a carbon source for initial growth of cultures since PAO∆mifR, PAO∆mifS, PAO∆mifSR and PAO∆rpoN strains exhibit growth defects in the presence of α-KG alone. When the cells reached an optical density at 600 nm (OD600) of 0.6–0.7 all the cultures were treated with 30 mM α-KG for 1 h. Post treatment, RNA was stabilized by addition of phenol-ethanol mixture . Stabilized RNA was then isolated using RNeasy Mini Kit (Qiagen, Inc Venio, Limburg, Netherlands) as per manufacturer’s protocol. Residual genomic DNA contamination was removed using RQ1 Rnase-free DNase (Promega, Madison, WI, USA) and RNA was repurified using Rneasy Mini Kit (Qiagen, Inc Venio, Limburg, Netherlands). Quality of purified RNA was assessed on a denaturing agarose gel (NorthernMax Gly, Ambion, Life Technologies, Carlsbard, CA, USA) and quantified at 260 nm (BioTEK, Synergy HT, Winooski, VT, USA). cDNA was then synthesized by annealing NS5 random primers to total purified RNA and subsequent extension was carried out using SuperScript III reverse transcriptase (Invitrogen, Life Technologies, Carlsbard, CA, USA).
qRT-PCR to study expression levels of PA5530 under α-KG induction was performed using Applied Biosystems Step One cycler and detection system with PowerSYBR Green PCR MasterMix with ROX (Applied Biosystems, Life Technologies, Carlsbard, CA, USA). In addition RNA was isolated from PAO1, PAO∆mifR, PAO∆mifS and PAO∆mifSR strains grown in M9 Minimal media supplemented with citrate (30 mM) without α-KG treatment, as described previously. qRT-PCR to study expression levels of genes encoding sigma-54 rpoN (PA4462), iso-citrate dehydrogenase (idh (PA2623) and icd (PA2624)), α-KG dehydrogenase complex (sucA (PA1585) and lpd3 (PA4829)) were done essentially as described above. The cycling conditions used were 95°C/2 minutes (holding); 40 cycles of 95°C/15 sec, 60°C/1 min (cycling); 95°C/15 sec, 60°C/1 min, 95°C/15 sec (0.6°C ramp) (melt curve). Expression was normalized to clpX (PA1802), whose expression was determined to remain constant between the samples and conditions tested .
Sequence analyses and domain organization studies were performed using the Simple Modular Architecture Research Tool (SMART)  and InterPro domain prediction database . mifS (PmifS) and PA5530 (PPA5530) promoter analyses and motif search was done using the ensemble learning method SCOPE and GLAM2 (Gapped Local Alignment of Motifs) [113,114]. Multiple sequence alignment was generated using ClustalW2 (http://www.ebi.ac.uk/Tools/msa/clustalw2/) and www.pseudomonas.com .
All data were analyzed for statistical significance using the Student’s t-test on GraphPad or Analysis of Variance (ANOVA) with post-hoc testing when appropriate, on IBM SPSS Statistics 22.0 statistical analysis software. Differences were considered to be significant at p- values < 0.05.
The authors thank the following individual for their intellectual input: Kyle Martins and Jeremy Chambers (Florida International University), Deepak Balasubramanian (Harvard Medical School), Lars Dietrich (Columbia University), and Elaine Newman (Concordia University, Canada). We would like to thank Dr. D. Haas from UNIL, Switzerland for kindly providing PA0ΔrpoN and PA0ΔrpoN::rpoN.
Conceived and designed the experiments: GT KM. Performed the experiments: GT HK EH LR. Analyzed the data: GT KM HK. Contributed reagents/materials/analysis tools: KM. Wrote the paper: GT KM.
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