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Correction: PRAME Is a Golgi-Targeted Protein That Associates with the Elongin BC Complex and Is Upregulated by Interferon-Gamma and Bacterial PAMPs

  • Frances R. Wadelin,
  • Joel Fulton,
  • Hilary M. Collins,
  • Nikolaos Tertipis,
  • Andrew Bottley,
  • Keith A. Spriggs,
  • Franco H. Falcone,
  • David M. Heery

Correction: PRAME Is a Golgi-Targeted Protein That Associates with the Elongin BC Complex and Is Upregulated by Interferon-Gamma and Bacterial PAMPs

  • Frances R. Wadelin, 
  • Joel Fulton, 
  • Hilary M. Collins, 
  • Nikolaos Tertipis, 
  • Andrew Bottley, 
  • Keith A. Spriggs, 
  • Franco H. Falcone, 
  • David M. Heery
PLOS
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Fig 2 is incorrect. Panels A-D are inadvertently missing. The authors have provided a corrected version here.

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Fig 2. PRAME localises to the Golgi network following LPS/IFNγ treatment.

(A) HEK293 cells (upper panels) were transiently transfected with PRAME-EGFP (green) and stained with α-PRAME antibody (red) to confirm the identity of the overexpressed EGFP fusion protein. U2OS cells (lower panels) were cotranfected with GFP (green) and PRAME-FLAG (red). Merged images indicate the extent of coincidence of the EGFP and α-PRAME signals, and nuclear DNA is indicated (blue). The right hand panels are western blots showing detection of GFP or PRAME-EGFP proteins in whole cell extracts of transfected U2OS cells. (B) Immunostaining of endogenous PRAME in HL60 cells using α-PRAME antibody following treatment with PBS, LPS/IFNγ or PGN/IFNγ for 4 hrs. (C) Immunostaining of endogenous PRAME in U937 cells with α-PRAME following treatment with LPS/IFNγ for 0, 1 and 4 hrs. (D) HL60 cells treated with LPS/IFNγ for 4 hrs and immunostained with α-Golgi 58K (green) and α-PRAME (red). Merged images show the extent of colocalisation of both proteins. For immunofluorescence (A–D), nuclear DNA was stained using Hoechst 33258 and images were captured using a LSM510 confocal laser scanning microscope. (E) Immunostaining of endogenous PRAME in HL60 cells using α-PRAME antibody following treatment with PBS or LPS/IFNγ for 4 hrs. (F) Quantification (n = 60) of the percentage of cells in (E) containing PRAME cytoplasmic foci in treated cells or controls. (G) Immunostaining of endogenous PRAME in MCF-7 cells using α-PRAME antibody.

https://doi.org/10.1371/journal.pone.0129297.g001

Reference

  1. 1. Wadelin FR, Fulton J, Collins HM, Tertipis N, Bottley A, Spriggs KA, et al. (2013) PRAME Is a Golgi-Targeted Protein That Associates with the Elongin BC Complex and Is Upregulated by Interferon-Gamma and Bacterial PAMPs. PLoS ONE 8(2): e58052. pmid:23460923