Mice were anesthetized and placed on mechanical ventilation as described in Fig 1. After one hour of mechanical ventilation, mice were injected intraperitoneally with PBS or NAM at doses of 50 mg/kg (NAM50+MV), 200 mg/kg (NAM200+MV) or 400 mg/kg (NAM400+MV). (A) Oxygen saturation was measured each hour. Significance is indicated for comparisons between NAM400 and NAM50 (** p < 0.01, *** p < 0.001) and between NAM400 and NAM200 (# p < 0.05, ### p < 0.001). Mice were euthanized at the end of 6 hours mechanical ventilation, and bronchoalveolar lavage (BAL) fluid was assayed for: (B) protein concentration and (C) neutrophil (PMNs) numbers and percentages of total BAL cells. PBS and NAM400 data are repeated from Fig 1 for comparison with NAM50 and NAM200. Data are representative of two separate experiments for NAM50 and NAM200, and four separate experiments for PBS and NAM400. Significance is indicated for comparisons between PBS and PBS+MV, and between PBS+MV and all NAM+MV doses. * p < 0.05, ** p < 0.01, *** p < 0.001.
Citation: Jones HD, Yoo J, Crother TR, Kyme P, Ben-Shlomo A, Khalafi R, et al. (2015) Correction: Nicotinamide Exacerbates Hypoxemia in Ventilator-Induced Lung Injury Independent of Neutrophil Infiltration. PLoS ONE 10(5): e0128735. https://doi.org/10.1371/journal.pone.0128735
Published: May 21, 2015
Copyright: © 2015 Jones et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited