There are a number of errors in the headings for Fig 1B, “Quenching by anti-Alexa Fluor mAbs.” “Beads coated with 1C1-A488” and “Beads stained with 1C1-A488” should be “Beads coated with 1C1-A594” and “Beads stained with 1C1-A594”. Please see the corrected Fig 1 here.
(A) Fluorescence of Alexa Fluor 488 (A488) on microbeads coated with 1C1-A488 or PC-3 cells stained with 1C1-A488 was quenched with a titration of the benchmark, a rabbit anti-A488 polyclonal, or 1 of 3 anti-A488 mAbs. One representative experiment of multiple is shown. (B) Fluorescence of Alexa Fluor 594 (A594) on microbeads coated with 1C1-A594 or PC-3 cells stained with 1C1-A594 was quenched with a titration of 1 of 3 anti-A594 mAbs. One representative experiment of multiple is shown. (A, B) Median fluorescence intensities (MFIs) at each anti-A488 or anti-A594 mAb concentration were normalized against a buffer control. The chimeric IgG1 isotype control was used as a non-quenching mAb control. The IC50 values (microgram/ml) of quenching and the corresponding 95% confidence intervals (95% CI) are listed for both the microbead- and cell-based titrations.
There are errors in the equation in the second to last sentence of the penultimate paragraph of the subsection “Flow cytometry-based internalization assay using cell surface fluorescence quenching.” The correct sentence should be: Internalized fluorescence was calculated from quenched and non-quenched sample data by correcting for incomplete surface quenching: 1 – (N1 – Q1)/(N1 – (N1Q0/N0)) with N1 = Unquenched MFI at each time point (t1); Q1 = Quenched MFI at t1; Q0 = Quenched MFI for the sample kept on ice (t0); N0 = Unquenched MFI at t0. The publisher apologizes for the error.
There are errors in the equation in the first sentence of the last paragraph of the subsection “Flow cytometry-based internalization assay using cell surface fluorescence quenching.” The correct sentence should be: Internalized fluorescence was plotted and a curve fit was obtained in Prism by nonlinear regression with the one-phase association equation: Y = Y0 + (Plateau – Y0) * (1 – exp(-K*x)) with Y0 = 0 when X (time) is zero and Plateau = 100. The publisher apologizes for the error.
Citation: The PLOS ONE Staff (2015) Correction: Quantitative Assessment of Antibody Internalization with Novel Monoclonal Antibodies against Alexa Fluorophores. PLoS ONE 10(5): e0128729. https://doi.org/10.1371/journal.pone.0128729
Published: May 15, 2015
Copyright: © 2015 The PLOS ONE Staff. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited