There is an error in the first sentence of the “RNA interference” subsection of the Materials and Methods. The correct sentence is: Synthetic siRNA targeting TCTP (target sequence 5’- CCATCACCTGCAGGAAACA-3’) was obtained from Dharmacon, while siRNA for luciferase (target sequence: 5’-AACTTACGCTGAGTACTTCGA-3’) was from Qiagen.
There is an error in the title of the fourth subsection of the Results. The correct title is: Down-regulation of TCTP Results in Down-regulation of Immune Response Genes in LNCaP Cells.
LNCaP cells were transfected with siRNA against TCTP or Luciferase for 72 h, RNA was isolated and used in global gene expression profiling as described in Materials and Methods. A. Venn diagram of the genes that are significantly regulated by gene expression profiling. B. Heatmap representation of genes up- or down-regulated in response to TCTP knockdown. Red and green represent up- and downregulated genes, respectively. C. List of the ten most significantly regulated genes with their accession numbers, definition and the ontology process for which they are implicated in. The fold change relative to Luciferase siRNA treated cells are indicated.
A-F. qPCR was used to assess expression of genes predicted to be differentially expressed in cells tranfected with Luc-siRNA versus TCTP-siRNA. The mRNA expression was normalized to GAPDH and was calculated relative to Luc-siRNA samples (set to 1). Experiments were carried out in triplicate. All error bars represent ±SEM. Statistical significance was assessed using two-tailed, paired Student’s t-test with *: P<0.05 and **: P<0.01 being considered as significant.
Citation: Kaarbø M, Storm ML, Qu S, Wæhre H, Risberg B, Danielsen HE, et al. (2015) Correction: TCTP Is an Androgen-Regulated Gene Implicated in Prostate Cancer. PLoS ONE10(5): e0128727. https://doi.org/10.1371/journal.pone.0128727
Published: May 14, 2015
Copyright: © 2015 Kaarbø et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited