There is an error in Fig 2 that appears to have occurred during the preparation of files after the manuscript was accepted. In Fig 2B, the blot for Brd4 incorrectly appears as a duplicate of P-Brd4. The authors have provided a corrected version of Fig 2 here.
Cells were either untreated or treated with 1 μM of JQ1 for 2 hours. (A) Chromatin Immunoprecipitation (ChIP) across MYC with anti-C-terminal Brd4 antibody. Each experiment was performed twice, analyzed in triplicate via real-time PCR and reported as the mean and standard deviation of the two experiments. A representation of the promoter area of MYC is provided for orientation. (B) Western blotting to detect (far left) Brd4 (~180 KD) and (middle) Brd4-S484/488-phos (P-Brd4, ~220 KD) was performed three times. A non-specific band detected with phopsho-Brd4 antibody is denoted with an asterisk. Typical results are shown with densitometry analysis relative to CREB expression, which is used as a normalization control (far right).
Citation: Fowler T, Ghatak P, Price DH, Conaway R, Conaway J, Chiang C-M, et al. (2015) Correction: Regulation of MYC Expression and Differential JQ1 Sensitivity in Cancer Cells. PLoS ONE 10(4): e0126328. https://doi.org/10.1371/journal.pone.0126328
Published: April 22, 2015
Copyright: © 2015 Fowler et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited