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Incidence of Cancer in ANCA-Associated Vasculitis: A Meta-Analysis of Observational Studies

  • Weifeng Shang,

    Affiliation Department of Nephrology, Tongji hospital affiliated to Tongji medical college, Huazhong University of Science and Technology, Wuhan, Hubei, China

  • Yong Ning,

    Affiliation Department of Nephrology, Tongji hospital affiliated to Tongji medical college, Huazhong University of Science and Technology, Wuhan, Hubei, China

  • Xiu Xu,

    Affiliation Department of Nephrology, Tongji hospital affiliated to Tongji medical college, Huazhong University of Science and Technology, Wuhan, Hubei, China

  • Menglan Li,

    Affiliation Department of Nephrology, Tongji hospital affiliated to Tongji medical college, Huazhong University of Science and Technology, Wuhan, Hubei, China

  • Shuiming Guo,

    Affiliation Department of Nephrology, Tongji hospital affiliated to Tongji medical college, Huazhong University of Science and Technology, Wuhan, Hubei, China

  • Min Han,

    Affiliation Department of Nephrology, Tongji hospital affiliated to Tongji medical college, Huazhong University of Science and Technology, Wuhan, Hubei, China

  • Rui Zeng,

    Affiliation Department of Nephrology, Tongji hospital affiliated to Tongji medical college, Huazhong University of Science and Technology, Wuhan, Hubei, China

  • Shuwang Ge ,

    geshuwang@tjh.tjmu.edu.cn (SG); xugang@tjh.tjmu.edu.cn (GX)

    Affiliation Department of Nephrology, Tongji hospital affiliated to Tongji medical college, Huazhong University of Science and Technology, Wuhan, Hubei, China

  • Gang Xu

    geshuwang@tjh.tjmu.edu.cn (SG); xugang@tjh.tjmu.edu.cn (GX)

    Affiliation Department of Nephrology, Tongji hospital affiliated to Tongji medical college, Huazhong University of Science and Technology, Wuhan, Hubei, China

Incidence of Cancer in ANCA-Associated Vasculitis: A Meta-Analysis of Observational Studies

  • Weifeng Shang, 
  • Yong Ning, 
  • Xiu Xu, 
  • Menglan Li, 
  • Shuiming Guo, 
  • Min Han, 
  • Rui Zeng, 
  • Shuwang Ge, 
  • Gang Xu
PLOS
x

Abstract

Objective

The purpose of this paper is to examine cancer incidence in patients with ANCA-associated vasculitis (AASV) derived from population-based cohort studies by means of meta-analysis.

Methods

Relevant electronic databases were searched for studies characterizing the associated risk of overall malignancy in patients with AASV. Standardized incidence rates (SIRs) with 95% confidence intervals (CIs) were used to evaluate the strength of association. We tested for publication bias and heterogeneity and stratified for site-specific cancers.

Results

Six studies (n = 2,578) were eventually identified, of which six provided the SIR for overall malignancy, five reported the SIR for non-melanoma skin cancer (NMSC), four for leukemia, five for bladder cancer, three for lymphoma, three for liver cancer, four for lung cancer, three for kidney cancer, four for prostate cancer, four for colon cancer and four for breast cancer. Overall, the pooled SIR of cancer in AASV patients was 1.74 (95%CI = 1.37–2.21), with moderate heterogeneity among these studies (I2 = 65.8%, P = 0.012). In sub-analyses for site-specific cancers, NMSC, leukemia and bladder cancer were more frequently observed in patients with AASV with SIR of 5.18 (95%CI = 3.47–7.73), 4.89 (95%CI = 2.93–8.16) and 3.84 (95%CI = 2.72–5.42) respectively. There was no significant increase in the risk of kidney cancer (SIR = 2.12, 95%CI = 0.66–6.85), prostate cancer (SIR = 1.45, 95%CI = 0.87–2.42), colon cancer (SIR = 1.26, 95%CI = 0.70–2.27), and breast cancer (SIR = 0.95, 95%CI = 0.50–1.79). Among these site-specific cancers, only NMSC showed moderate heterogeneity (I2 = 55.8%, P = 0.06). No publication bias was found by using the Begg’s test and Egger's test.

Conclusions

This meta-analysis shows that AASV patients treatment with cyclophosphamide (CYC) are at increased risk of late-occurring malignancies, particularly of the NMSC, leukemia and bladder cancer. However, there is no significant association between AASV and kidney cancer, prostate cancer, colon cancer and breast cancer. These findings emphasize monitoring and preventative management in AASV patients after cessation of CYC therapy is momentous.

Introduction

The anti-neutrophil cytoplasm antibody associated vasculitides (AASVs), including graulomatosis with polyangiitis (GPA, Wegener’s granulomatosis) and microscopic polyangiitis (MPA), are a group of multisystem disorders characterized by necrotizing inflammation of small blood vessels[1]. Although, AASV involves small vessels with predilection for the kidneys, lungs, and peripheral nervous system in most of the patients, any organ system can be affected. The disease usually presents in the middle aged or elderly, with a peak incidence of 65 per million/year in those aged 65–74 years[2]. There is a reported mortality of 80% at 1 year in untreated patients[2]. Since the late 1970s, the introduction of glucocorticoids and cyclophosphamide (CYC) as standard treatments has improved the prognosis considerably[3]. An analysis of four multicenter trials reported cumulative survival at 1, 2 and 5 years of 88%, 85% and 78%, respectively[4]. With standard therapy regimen, remission can be induced in about 70–90% of patients[5]. Nevertheless, 25–50% of the patients will relapse[6], leading to increased duration and quantity of CYC which would cause toxic effects especially for cardiovascular and cancer morbidity[7]. The latter has drawn more attention recently. Much effort has been devoted to this field which demonstrated the SIRs for overall cancer has increased to more than 1.6 associated with AASV[6,813], but the risk of overall cancer appeared to be somewhat reduced in one study (SIR = 0.8, 95%CI = 0.5–1.4)[14]. Moreover, cancer type-specific analyses demonstrated an significantly increased risk of non-melanoma skin cancer (NMSC)[812], leukemia[912], and bladder cancer[6,912,15] in some studies. However, the reported risk is different. Recently, the comprehensive review performed by Mahr et al.[16] has shown the current understanding of the potential link between AASV and the occurrence of cancer, but no meta-analysis has been used to examine the relationship. Given the fact that individual studies may have insufficient statistical power because of sample size, therefore, we undertook the present meta-analysis to quantitatively confirm the incidence of cancer in AASV patients versus the general population, which may provide a realistic perspective on risk in the clinical setting.

Materials and Methods

Search strategy and study selection

A PubMed and EMBASE databases were searched systematically for all articles published before September 1st, 2014. The search terms used were “cancer and ANCA-associated vasculitis”,“cancer and AASV”,“cancer and WG”,“cancer and GPA”,“cancer and MPA”, “epidemiology and ANCA-associated vasculitis”. Two investigators (WS and SG), using these parameters, independently filtered out all the eligible articles and hand-searched references of retrieved papers for additional available studies. We did not include unpublished or abstract only publications. No restriction was placed on language. Conflicting results were resolved by consensus and endnote was used to merge retrieved citations.

Inclusion criteria

Included studies met the following criteria: (1) they were cohort studies that estimating the influence of AASV on cancer risk with odds ratio (OR), risk ratio (RR), hazard ratio (HR), or standardized incidence/mortality rate(SIR/SMR) and their 95% confidence intervals (CIs) of overall cancer. (2) defined AASV as one of the exposure interests and cancer as one of the outcome of interests.

Exclusion criteria

The exclusion criteria included: reviews, case reports, conference publications, cross-sectional studies, studies limiting the population age, studies not specifying the types of tumor, and studies focusing on demonstrate incidence of cancer before the diagnosis of AASV. If a cohort study was reported in more than one publication, we chose the largest sample size or the latest article.

Data extraction and Quality evaluation

The following data were extracted independently by two investigators (WS and SG) from the included studies: the authors, publication year, country, study design, AASV phenotypes studied, period of follow-up, mean/median observation period, cumulative observation period, number of patients studied, number of cancers observed in the cohort, patients’ gender and age, and SIR with its 95% CI, gender of cancer patients, main therapy. When needed, we contacted the original author for clarification. This study complied with meta-analysis of observational studies in Epidemiology (S1 MOOSE Checklist)[17]. The quality of each study was independently evaluated by two investigators (WS and SG) using the Newcastle-Ottawa Scale (NOS)[18]. The NOS, including selection, comparability and outcome, is a scale for assessing the quality of published non-randomized studies. The study which met at least five NOS criteria was considered to be a high quality study. Discrepancies between investigators were solved by consensus.

Data Synthesis and Analysis

In this meta-analysis, we collected SIR with 95%CI to combine the data and assessed heterogeneity of the mean difference with the Chi-squared based Q-statistic test. If the P value of the heterogeneity Q-statistic was less than 0.10, the random-effects model was used to calculate the pooled SIRs[19]. On the contrary, the fixed-effects model was selected[20]. We also quantified the effect of heterogeneity using the I2 index[21]. I2 values of 25%, 50% and 75% indicate low, moderate and severe statistical heterogeneity, respectively. Publication bias was evaluated using the Begg’s test and Egger’s test[22,23]. P value <0.10 was considered significant. All analyses were performed in Stata 10.0 (College Station, TX, USA).

Results

Studies included in the meta-analysis

As shown in Fig 1, our initial search rendered 2,762 potentially relevant articles, from which six cohort studies were included[812,14]. Three studies were monocentric cohorts[8,12,14], two study nationwide registry linkage[9,10], and one was a multinational study[11]. Among these studies, two were carried out in Sweden, one in Germany, one in Poland, one in Denmark and one in Europe and Mexico. They were published between 1998 and 2013. Mean or median observation period for ascertained cases ranged from 4.58 to 7 years. In this meta-analysis, we are including 258 cases of cancers identified in a total cohort of 2,578 individuals, 1,191 women (46%) and 1,387 men (54%), with a diagnosis of AASV selected characteristics of the included studies are shown in Table 1. While Table 2 shows the detailed ratings of study quality for NOS.

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Table 1. Characteristics of studies of ANCA-associated vasculitis (AASV) and cancer incidence.

https://doi.org/10.1371/journal.pone.0126016.t001

Overall cancer risk in AASV

The SIR of cancers within the six individual study populations ranged between 0.8 and 2.5, with an overall meta-analytical SIR of 1.74 (95%CI = 1.37–2.21), and the heterogeneity was moderate (I2 = 65.8%, P = 0.012). The forest plot of SIR is shown in Fig 2.

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Fig 2. Forest plot of association between ANCA-associated vasculitis (AASV) and overall cancer.

https://doi.org/10.1371/journal.pone.0126016.g002

Organ-specific Cancer Associated with AASV

We performed subgroup analyses for all site specific cancers that have been reported by more than one included study (Table 3). This subgroup analyses did not show evidence of significant heterogeneity (I2 = 0) except for the NMSC (I2 = 55.8%, P = 0.06). The result revealed that NMSC, leukemia and bladder cancer were more frequently observed in patients with AASV than in the entire population, with SIR of 5.18 (95%CI = 3.47–7.73), 4.89 (95%CI = 2.93–8.16) and 3.84 (95%CI = 2.72–5.42) respectively, followed by lymphoma (SIR = 3.79, 95%CI = 1.87–7.69), liver cancer (SIR = 3.50, 95%CI = 1.45–8.43), lung cancer (SIR = 1.67, 95%CI = 1.07–2.60). However, there was no significant increase in the risk of kidney cancer (SIR = 2.12, 95%CI = 0.66–6.85), prostate cancer (SIR = 1.45, 95%CI = 0.87–2.42), colon cancer (SIR = 1.25, 95%CI = 0.66–2.38), and breast cancer (SIR = 0.95, 95%CI = 0.50–1.79). In organ-specific cancer with only one study, formal meta-analyses were not performed. However, it is still worth noting that the greatly high SIRs for cancers of the testis (45.7)[8], vulva (32.9)[8] and nose and ear (14.1)[9] reported relating to AASV.

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Table 3. Pooled site-specific cancer risks in patients with ANCA-associated vasculitis (AASV).

https://doi.org/10.1371/journal.pone.0126016.t003

Sensitivity analysis

The results of this meta-analysis were not affected by sensitivity analysis, which were performed by excluding one study at a time. When any individual study was excluded, the corresponding pooled SIRs were not substantially altered (data not shown). The statistically similar results indicated this meta-analysis was relatively stable.

Heterogeneity and reporting bias

In the overall cancer and NMSC groups, moderate heterogeneity between studies was observed (all P <0.10). When the study by Holle et al.[14] was deleted, the P value of heterogeneity for the overall cancer group was 0.414, indicating that this study may be the source of heterogeneity in the overall cancer group. As for the NMSC group, the P value of heterogeneity was 0.395 after removing the study by Heijl et al.[11]. The study might contribute to the heterogeneity in the the NMSC group.

The P value for Begg’s test was 0.707 (continuity corrected) and that for Egger’s test was 0.367, suggesting a low probability of publication bias.

Discussion

To the best of our knowledge, this study is the first meta-analysis to present cancer incidence in patients with AASV. We confirmed AASV was associated with an increased risk of cancer with a SIR of 1.74 (95%CI = 1.37–2.21).

There was an moderate heterogeneity in this meta-analysis. We consider that the main source of heterogeneity was the difference in follow-up time, as the mean follow-up varied in the studies. Heijl et al.[11] reported that cumulative overall cancer incidence rates were 8% and 13% at 5 and 8 years, respectively. In addition, the study by Holle et al.[14] would play an important part in heterogeneity. When the study was excluded, the heterogeneity disappeared. An increased incidence of malignancies in AASV was not found in the study[14], which may also be partly explained by the strict use of mesna to avoid bladder cancer and by the shorter average follow-up period of 3.9 years from cohort 3 than that in other cohorts. What’s more, different AASV types and sex ratio might contribute to the heterogeneity in our study. About 65% of the samples were patients with the GPA[9,10,14], 24% were with mixed GPA/MPA[8,11], and others with Pulmonary vasculitis[12]. The reported SIRs for cancers at all sites were 1.92(95%CI = 1.31–2.71) and 1.20(95%CI = 0.71–1.89) for GPA and MPA, respectively[11]. Interestingly, men had a slightly higher relative risk of cancer than women[9,11,14]. Last, immune inhibitors and its dosage were also the source of heterogeneity. In the material presented by Heijl et al.[11], the lower CYC exposure, the lower SIR for cancers in any site. Similarly, Faurschou et al.[10] found that the risk of these malignancies was not increased for patients who never received CYC or for patients treated with cumulative CYC doses ≤ 36 g. Furthermore, Silva et al.[13] found SIR for solid malignancies of 3.92 (95% CI = 1.69–7.72) in the etanercept group among WG patients as compared to the general population, suggesting malignancy with a strong association with etanercept use.

We also observed a strongly increased risk of cancer in this paper, the risk being most pronounced for NMSC, leukemia and bladder cancer.

In regard to NMSC in this meta-analysis, a 5.18–7.73-fold increased risk is seen in AASV patients compared to the general population. The observed association between AASV and NMSC may result from the following major aspects. Firstly, most of the skin cancers occurred in sun-exposed areas, typically in the facial region[10], explained by that ultraviolet light is the main environmental cause of NMSC. Secondly, the direct causal association of AASV with NMSC may be mediated by intense immunosuppressive therapy[10]. For example, in the Westman et al.’s study[8], no case of skin cancer was observed during the first year of follow-up, however, the risk for NMSC was significantly associated with azathioprine therapy for more than 12 mouths (16-fold increase) and with glucocorticoids for more than 48 mouths (20-fold increase). Alternatively, subsequent studies in AASV were consistent with observations in the Westman et al.’s study[10,12]. Furthermore, one study found that azathioprine might hasten the induction of NMSC by sensitising the skin cell genome to ultraviolet A radiation[24]. Therefore, patients with AASV should reduce sun exposure and use sun block regularly to decrease the risk of NMSC.

With respect to leukemia risk, the results suggest an approximate 4.89 to 8.16 increased risk in patients with AASV. Some studies revealed a high risk of leukemia following treatment with CYC in AASV patients, and the risk of secondary malignancies was shown to be dependent upon the cumulative dose of CYC received[25]. Faurschou et al.[10] has demonstrated a 59-fold increased SIR of acute myeloid leukemia (AML) in patients treated with a cumulative dose of CYC > 36 g. In contrast, no case of leukemia due to milder or shorter duration of immunosuppression was found in the study by Westman et al.[8]. Besides, in the material presented by Faurschou et al.[10], the high risk of AML (SIR = 19.6, 95%CI = 4.0–57) was observed, with long latency periods of seven to sixteen years. This finding is in agreement with observations by Zycinska and colleagues, who demonstrated the risk of leukemia significantly increasing between 5 and 10 years of the follow-up observation[12]. Consequently, Patients with AASV should undergo the long-term follow-up with blood routine examination after CYC therapy to early detection of leukemia.

There is an increased SIR of bladder cancer (SIR = 3.84, 95%CI = 2.72–5.42) without heterogeneity (I2 = 0, P = 0.809). Our study and several studies[6,15] have demonstrated that the risk for bladder cancer was associated with the cumulative dose of CYC administered. Indeed, Hoffman et al.[6] reported a 33-fold and Talar-Williams et al.[15] a 31-fold increased risk for bladder cancer described in a cohort of WG patients followed at the NIH (National Institutes of Health, Bethesda, Maryland). The higher incidence of bladder carcinomas among the NIH patients might reflect that these patients generally received higher cumulative doses of CYC (>50 g for 64% of patients) than the patients (24% of patients) in Faurschou et al.’s study[10]. Besides, bladder cancers have been reported by another study with, like observations in hematology, a median latency period of 10.4 year after a first CYC exposure[12]. Other factors may have contributed to predict the development of bladder cancer in AASV. Microscopic non-glomerular hematuria has been recognised to be statistically significantly associated with the development of bladder cancer[15]. Although a history of smoking tobacco was not found to be an independent risk factor for the development of bladder cancer, tobacco smokers may develop bladder cancer at lower doses and earlier than non-smokers[15]. These results contribute to defining risk populations who should benefit from close and prolonged screening for bladder cancer. Moreover, oral administration of CYC was also an important predictor of increased bladder cancer risk, as a result of a French study[26]. It is also recommended that intravenous CYC should be systematically co-prescribed with mesna and hyperhydration and additional mesna prescription should be considered for oral CYC with prolonged treatment (>4–6 months)[27].

The results of our meta-analysis show no statistically significant association between AASV and kidney cancer, prostate cancer, colon cancer and breast cancer. Interestingly, kidney cancer is of note. Although its CI is wide and include the null value, either misclassification or surveillance-related bias can not be excluded, the finding may declare a carcinogenic effect of the disease process itself, as previously indicated by case reports[28]. In addition, studies of AASV reported strikingly high SIRs for cancers of the testis[8], vulva[8] and nose and ear[9], which relied on single cases and may reflect chance findings. Although not addressed in our study, the incidence of cervical neoplasia is increased in other diseases treated with immunosuppressants[29]. Thus, cervical screening is of great importance in women exposed to immunosuppressive therapies.

Several potential reasons relating to AASV that cause the increased SIRs for cancers are listed as follows. First, the used cytotoxic (predominantly CYC) therapy may increase the risk of malignancy. And in agreement of our finding, an umbrella review of 74 meta-analyses demonstrated commonly used medications including CYC may have potential increased risk of cancer[30]. Therefore, we should select a rational approach for different disease scenarios (try to avoid overuse of CYC) and focus on adverse effects of therapy[31]. Second, the dysfunction in immune system may raise the cancer risk[32]. Third, longstanding immune activation in patients with AASV may be oncogenic[33]. Fourth, vasculitis may be a paraneoplastic phenomenon, for instance, polyarteritis nodosa have been described in association with hairycell leukemia, owing to starting at the same time, concurrently improvement and the same nature[34]. Finally, some reports of cancer in association with AASV might be attributable to a coincidental association related to detection bias.

Several limitations of this meta-analysis should be acknowledged. Firstly, moderate heterogeneity was detected in the overall cancer and NMSC groups and publication bias, selection bias and a residual confounding bias may have existed although we cannot evaluate these hypotheses. Secondly, all of the studies included were partially representative as western countries with the Caucasian, and therefore, extrapolating results to other parts of the world should be interpreted cautiously. Thirdly, this meta-analysis included studies with different designs, observation period and sample size, which could introduce inherent limitations. Fourthly, due to limited data, we could not assess some confounding factors such as age and smoke. Fifthly, ANCA are now widely used as diagnostic markers for AASV, which may result in the early diagnosis of AASV, therefore it might have some bias in our final conclusions. Lastly, we could not judge whether cancer was a potential trigger or cause of AASV or a mere coincidence.

In summary, our study confirms a high incidence of cancer in the AASV population, specifically for NMSC, leukemia and bladder cancer, which might be associated with CYC use, particularly higher cumulative doses. Therefore the close follow-up is required. Periodic urine analysis, blood analysis and cervical screening should be performed. And smoking cessation and avoiding sun exposure simultaneously are recommended. Cystoscopy, bladder ultrasonography and skin biopsies should be advised in necessity. Further investigations are required to extract the accurate relations for the cancer risk to the cumulative doses. There is a continuing need for effective alternatives to toxic CYC treatment. Future studies should also focus on the underlying mechanisms between AASV and cancer risk.

Author Contributions

Conceived and designed the experiments: GX S. Ge. Performed the experiments: WS S. Ge YN. Analyzed the data: WS S. Ge YN RZ. Contributed reagents/materials/analysis tools: MH ML XX S. Guo. Wrote the paper: WS S. Ge MH.

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