Data are presented as mean±SDs. There was a significant difference in the percentage of dye-stained cells between the Cx50WILD and Cx50V44A groups in the Ca2+-free environment (P<0.001, marked with ***); there was also a significant difference between the Ca2+-free Cx50WILD and the 1.2 mM Ca2+/300 μM FFA Cx50WILD group (P<0.001, marked with ***).
Please see the complete, corrected Fig 5 here.
The PI dye uptake assay revealed similar results as the DAPI dye uptake assay. The three pictures in the last line show the Cx50 protein, PI-stained nuclei (nucleoli)/cytoplasm, and merged look of the wild-type cells in Ca2+-free HBSS. Scale bar: 20 μm.
Citation: Zhu Y, Yu H, Wang W, Gong X, Yao K (2015) Correction: A Novel GJA8 Mutation (p.V44A) Causing Autosomal Dominant Congenital Cataract. PLoS ONE 10(5): e0125949. doi:10.1371/journal.pone.0125949
Published: May 13, 2015
Copyright: © 2015 Zhu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited