(A) Cleaved caspase-3 (CASP3; green), an apoptosis marker, was used to stain sections of RWPE-1, RWPE-2 and LNCaP microaggregates. (B) Due to the absence of cleaved caspase-3 in the small LNCaP aggregates, a large microwell (800 μm ×800 μm ×800 μm) was used to create large LNCaP microaggregates with an apoptotic core (CASP3 Lge). (C) The diameter of the LNCaP microaggregates (Sml) was compared with LNCaP microaggregates grown in the large microwells (Lge). A minimum of 50 aggregates were measured per condition. (D) Ki67 (green) was also used to stain proliferating cells within the large LNCaP aggregate. Nuclei were stained with DAPI (magenta); scale bar is 100 μm.
LNCaP cells (50,000 cells/well) were treated with docetaxel over 48 hrs (A) or 72 hrs (B) at day 2 of growth either in 2D and 3D. Alamar blue was used to assess cell viability. Mean +/- SE, n = 4 biological replicates *P<0.05; the data shown is representative of three independent experiments. (C) Phase contrast images show the effects of docetaxel after 72 hrs on the morphology of the microaggregates (3D) compared to cells grown in monolayer (2D).
- 1. Chambers KF, Mosaad EMO, Russell PJ, Clements JA, Doran MR (2014) 3D Cultures of Prostate Cancer Cells Cultured in a Novel High-Throughput Culture Platform Are More Resistant to Chemotherapeutics Compared to Cells Cultured in Monolayer. PLoS ONE 9(11): e111029. doi: 10.1371/journal.pone.0111029. pmid:25380249
Citation: Chambers KF, Mosaad EMO, Russell PJ, Clements JA, Doran MR (2015) Correction: 3D Cultures of Prostate Cancer Cells Cultured in a Novel High-Throughput Culture Platform Are More Resistant to Chemotherapeutics Compared to Cells Cultured in Monolayer. PLoS ONE 10(4): e0125641. doi:10.1371/journal.pone.0125641
Published: April 22, 2015
Copyright: © 2015 Chambers et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited