S2 Fig. Development of engineered constructs for disrupting gene targets.
Internal fragments of the p48 (lane 1, 392 bp), type II restriction endonuclease (lane 2, 462 bp) and xer1 (lane 3, 251 bp) genes were amplified from M. bovis strain PG45 with appropriate primers and inserted between the NotI and PstI sites of the IRR based oriC plasmid. To promote homologous recombination, the recA gene was amplified from M. gallisepticum strain S6 and cloned between the PstI and SalI cleavage sites of the construct.
Citation: Sharma S, Citti C, Sagné E, Marenda MS, Markham PF, Browning GF (2015) Correction: Development and Host Compatibility of Plasmids for Two Important Ruminant Pathogens, Mycoplasma bovis and Mycoplasma agalactiae. PLoS ONE 10(4): e0125268. doi:10.1371/journal.pone.0125268
Published: April 10, 2015
Copyright: © 2015 Sharma et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited