(A) NP-40-soluble and (B) NP-40-insoluble fractions. Gray: calcium-free medium, black: Zn++-enriched medium, white: Zn++-enriched medium treated with STb for 6, 12 and 24 h. Lower panel: Immunoblot showing claudin-1 and GAPDH used to evaluate their relative amounts. NP-40 cell extracted proteins were separated on a 12% acrylamide SDS-PAGE and immunoblotted with anti-claudin-1 and anti-GAPDH antibodies. The calcium-free medium was Zn++ -enriched (1.8 mM). There was no significant difference in claudin-1 dislogment rate under Zn++-enriched condition compared to calcium-free medium. After 24 h, claudin-1 dislogment was observed as seen before in calcium-free medium (n = 3) (p<0.001). CLDN-1: claudin-1, GAPDH: Glyceraldehyde 3-phosphate dehydrogenase. Letters on top of the bars when different indicates a statistical difference between the treatments.
Cells grown in calcium-free and zinc-enriched (1.8 mM) media were compared after 6 and 24 h. Confocal microscopy was used to analyze the distribution of actin filaments stained with FITC-phalloidin. Zinc-enriched medium had no visible effect on the actin organization whereas in zinc-enriched medium STb provoked actin condensation after 24 h. In calcium-free medium, actin condensation was observed only after 24 h (Data not shown) Bar, 30 μm.
Citation: The PLOS ONE Staff (2015) Correction: Escherichia coli STb Enterotoxin Dislodges Claudin-1 from Epithelial Tight Junctions. PLoS ONE 10(3): e0118983. https://doi.org/10.1371/journal.pone.0118983
Published: March 3, 2015
Copyright: © 2015 The PLOS ONE Staff. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited