Lysine acetylation is a reversible post-translational modification (PTM) that has been detected on thousands of proteins in nearly all cellular compartments. The role of this widespread PTM has yet to be fully elucidated, but can impact protein localization, interactions, activity, and stability. Here we present the first proteome-wide survey of lysine acetylation in cortical astrocytes, a subtype of glia that is a component of the blood-brain barrier and a key regulator of neuronal function and plasticity. We identified 529 lysine acetylation sites across 304 proteins found in multiple cellular compartments that largely function in RNA processing/transcription, metabolism, chromatin biology, and translation. Two hundred and seventy-seven of the acetylated lysines we identified on 186 proteins have not been reported previously in any other cell type. We also mapped an acetylome of astrocytes infected with the brain parasite, Toxoplasma gondii. It has been shown that infection with T. gondii modulates host cell gene expression, including several lysine acetyltransferase (KAT) and deacetylase (KDAC) genes, suggesting that the host acetylome may also be altered during infection. In the T. gondii-infected astrocytes, we identified 34 proteins exhibiting a level of acetylation >2-fold and 24 with a level of acetylation <2-fold relative to uninfected astrocytes. Our study documents the first acetylome map for cortical astrocytes, uncovers novel lysine acetylation sites, and demonstrates that T. gondii infection produces an altered acetylome.
Citation: Bouchut A, Chawla AR, Jeffers V, Hudmon A, Sullivan WJ Jr (2015) Proteome-Wide Lysine Acetylation in Cortical Astrocytes and Alterations That Occur during Infection with Brain Parasite Toxoplasma gondii. PLoS ONE 10(3): e0117966. doi:10.1371/journal.pone.0117966
Academic Editor: Silvia N. Moreno, Univ. Georgia, UNITED STATES
Received: October 27, 2014; Accepted: November 20, 2014; Published: March 18, 2015
Copyright: © 2015 Bouchut et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Data Availability: Raw data files have been uploaded to Dryad (10.5061/dryad.gb291).
Funding: Support for this study was provided by funding from the National Institutes of Health (http://www.nih.gov/), NS078171 (AH) and AI106435 (to WJS). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Astrocytes constitute a major subset of glial cells that carry out a wide variety of critical operations in the mammalian brain. It is well-established that astrocytes play a supportive role for neurons by providing metabolic support, releasing and taking up neurotransmitters, and maintaining extracellular ionic concentrations. Emerging studies suggest that in addition to the long-established structural role astrocytes play in the maintenance of the blood-brain barrier (BBB), astrocytes are key players in neuronal signaling, brain repair, and immune responses . In response to infection, astrocytes release cytokines and chemokines to modulate effector cells. There is also evidence that infection can alter astrocyte function; for example, HIV-infected astrocytes develop abnormal end-feet connections that lead to perturbations in the BBB . Other intracellular microbes capable of infecting astrocytes include Listeria monocytogenes and Toxoplasma gondii [3–5], but how they may modulate astrocyte function has yet to be defined.
T. gondii is an obligate, intracellular parasite belonging to the phylum Apicomplexa, which also includes other notorious protozoan pathogens such as Plasmodium spp. (malaria) and Cryptosporidium spp. (cryptosporidiosis). Up to a third of the world’s population is infected with T. gondii, which can reactivate as life-threatening disease in immunocompromised individuals. In addition, T. gondii infection during pregnancy can cause congenital birth defects, including blindness or hydrocephalus . T. gondii is commonly transmitted to virtually any warm-blooded vertebrate through oocysts expelled by its definitive host (felines) or bradyzoite-containing tissue cysts residing in undercooked meat . The tachyzoite stage of the parasite’s life cycle is characterized by rapid proliferation (doubling time of 6–10 hours) in any nucleated cell and can cause acute disease associated with tissue destruction. In immune competent hosts, the tachyzoites are induced to differentiate into bradyzoites, which are believed to be largely quiescent for the remainder of the host’s life . Attenuation of host immunity can lead to chronic reactivated infection mentioned above.
T. gondii can traverse the BBB and activate astrocytes as early as 10 days post-infection . While tissue cysts are predominantly seen in neurons during chronic infection, they also occur in microglia and astrocytes during earlier stages of infection . Astrocytes execute several immune functions that are involved in the intracerebral immune response to T. gondii. With microglia and cerebral microvascular endothelial cells, astrocytes form the IFNγ effector cell population that helps control tachyzoite replication in the brain. IFNγ-activated astrocytes significantly inhibit the growth of tachyzoites in mice via an Immunity-Related GTPase (IRG)-mediated mechanism [11,12] and by expressing pro-inflammatory agents [13–15].
To better understand host-parasite interactions, transcriptional profiling and proteomics analyses have been carried out on T. gondii-infected versus uninfected cells. T. gondii infection clearly modulates the host cell, resulting in dramatic changes in host gene expression [16–18] and protein levels [19,20]. Data from Saeij et al. reveals that message levels for several lysine acetyltransferases (KATs) and lysine deacetylases (KDACs) are significantly increased (HDAC2 and HAT1) or decreased (SIRT5 and MYST4/MORF) in infected cells . Modulation of KATs and/or KDACs by intracellular pathogens could impact the host cell transcriptome through histone (de)acetylation, thereby modifying the host cell milieu to favor progression of the parasite’s life cycle.
The alteration of host KATs and KDACs is also likely to alter the acetylation status of non-histone proteins as well; we and others have recently found that lysine acetylation is an abundant post-translational modification (PTM) that occurs on thousands of proteins of diverse function throughout multiple cellular compartments . So-called “acetylomes” have now been mapped for several organisms, including bacteria [22,23], plants [24,25], Saccharomyces cerevisiae  Drosophila melanogaster , human cells [28,29], and the protozoan parasites T. gondii and Plasmodium falciparum [30–32]. In this study, we present the first proteome-wide analysis of lysine acetylation in cortical astrocytes. We also determined the acetylome of T. gondii-infected astrocytes, which contained differences relative to the acetylated proteins found in uninfected astrocytes. Our findings add a new layer of complexity to the mechanisms intracellular pathogens may employ to manipulate their host. These studies provide a foundation for follow-up investigations aimed at determining the role of lysine acetylation on individual target proteins and how this impacts astrocytic function as well as T. gondii pathogenesis.
Materials and Methods
Astrocytes were cultured as previously described . In brief, cortices from postnatal day 1–2 Sprague-Dawley rat pups were enzymatically digested and triturated. Cortical cells were then resuspended in growth media (Dulbecco’s modified Eagle medium (DMEM) containing 5% NuSerum, penicillin 10 units/mL, streptomycin (10μg/ml), L-glutamax (2mM) and B-27) at a density of 2.5 million cells/mL, and resuspended onto 100 μg/ml poly-D-lysine (PDL) coated 10cm dishes. Cells were maintained in humidified incubators at 37°C under 5% CO2. Cells were fed every 2–4 days and when the cultures became confluent (7–8 days in vitro (DIV)), plates were shaken to remove oligodendrocytes and microglia. After a brief wash, trypsin was used to passage the astrocytes, which were subsequently split onto PDL-coated 10cm dishes (for proteomics or western blotting) or glass coverslips (for immunofluorescence). Similar to previously published findings , cultures are negative for MAP-2 (neuronal marker), OX-42 (microglial marker), Olig-2 (oligodendrocyte marker) and GFAP positive (>98%, astrocyte marker).
T. gondii culture and astrocyte infection
T. gondii were maintained in human foreskin fibroblasts (HFF; (ATCC CRL-1634)) using DMEM supplemented with 10% heat-inactivated fetal bovine serum (Invitrogen). RH strain tachyzoites cultured in HFFs were then physically separated from host cells by passage through a 23G syringe needle and purified from host cell debris using a 3.0 μm filter . Following centrifugation, parasites were resuspended in astrocyte growth medium (see above) and used to infect astrocyte monolayers at a multiplicity of infection (MOI) of 10 for 10 h. Several independent preparations had to be pooled in order to obtain 15 mg of protein lysate necessary to generate the astrocyte acetylomes.
Immunoaffinity enrichment of lysine-acetylated peptides
Harvested astrocytes were washed in PBS and resuspended in urea lysis buffer (9.0 M urea, 20 mM HEPES pH 8.0, 2.5 mM sodium pyrophosphate, 1 mM β-glycerol phosphate, 1 mM sodium orthovanadate) freshly supplemented with 10 mM sodium butyrate, a lysine deacetylase (KDAC) inhibitor. Sonicated lysates were centrifuged for 15 min at 4°C at 20,000 x g. Supernatants were collected and reduced with 4.5 mM DTT for 30 min at 55°C, followed by alkylation with iodoacetimide and dilution with 20 mM HEPES, pH 8.0 to normalize protein concentration across all samples. After digestion with 10 μg/mL trypsin-TPCK in 1.0 mM HCl, peptide lysates were acidified with 1% TFA and peptides were desalted over SEP PAK Classic C18 columns (Waters). Peptides were eluted with 40% acetonitrile in 0.1% TFA, dried under vacuum, and stored at-80°C.
Acetylated peptides were enriched using a pan-specific anti-acetyl-lysine antibody (CST #9895, Cell Signaling Technology) bound to 50 mL packed protein A agarose beads (Roche). Lyophilized peptides were resuspended in MOPS (morpholinepropane sulfonic acid) IAP buffer (50 mM MOPS (pH 7.2), 10 mM KH2PO4, 50 mM NaCl) and centrifuged for 5 min at 12,000 rpm. Supernatants were mixed with anti-acetyl-lysine beads for 2.5 h at 4°C and then centrifuged for 30 s at 5,400 rpm at 4°C. Beads were washed in MOPS IAP buffer, then in water, prior to elution of the peptides with 0.15% TFA. In preparation for analysis, the peptides were desalted over Empore C18 tips (Sigma) and eluted with 60% acetonitrile in 0.1% TFA.
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was performed at Cell Signaling Technology (Danvers, MA). Peptides were loaded directly onto a 10 cm x 75 μm PicoFrit capillary column packed with Magic C18 AQ reversed-phase resin. The column was developed with a 90-minute linear gradient of acetonitrile in 0.125% formic acid delivered at 280 nL/min. Tandem mass spectra were collected from duplicate samples in a data-dependent manner with an LTQ Orbitrap VELOS mass spectrometer using a top 20 method, a dynamic exclusion repeat count of 1 and a repeat duration of 35 s. MS/MS spectra were evaluated using SEQUEST 3G and the SORCERER 2 platform from Sage-N Research (v4.0, Milpitas CA) . Searches were performed against the most recent update of the NCBI Rattus norvegicus/Toxoplasma gondii combined database with mass accuracy of +/-50 ppm for precursor ions and 1 Da for product ions. Results were filtered with mass accuracy of +/− 5 ppm on precursor ions and presence of an acetylated lysine. The mzXML, Dtas, and Out files associated with this study are available upon request. Label-free quantification of individual acetylation sites was performed as previously described, with the fold change for each identified acetylated lysine was calculated by comparing changes in parent peptide ion intensities between uninfected and infected samples [36,37].
Data analysis and bioinformatics
Acetylated proteins were classified according to gene ontology (GO) annotations by Uniprot (http://www.uniprot.org) . Cellular localization data were also extracted from Uniprot. The secretome analysis was performed using Secretome 2.0 server (http://www.cbs.dtu.dk/services/SecretomeP) . Amino acid sequence motifs were analyzed using WebLogo 3.4 (http://weblogo.threeplusone.com/create.cgi) .
Immunofluorescence assays were performed as previously described . Briefly, astrocyte monolayers grown on coverslips were inoculated with RH strain tachyzoites. After removal of culture medium, infected HFFs were fixed in 4% paraformaldehyde for 10 min and then were permeabilized with 0.3% Triton X-100 for 10 min. For visualization of α-tubulin or α-tubulin acetylated at K40, mouse monoclonal anti-α-tubulin antibody (clone DM1A, Sigma T6199) and monoclonal anti-acetylated tubulin antibody (clone 6–11B-1, Sigma T7451) were applied at 1:2,000 followed by goat anti-mouse Alexa Fluor 488 secondary antibody at 1:2,000 (Invitrogen, A-11001). Nuclei were visualized through co-staining with 4′,6-diamidino-2-phenylindole (DAPI).
Astrocytes were obtained from postnatal rat pups (DIV1–2) using an approved protocol (10354) from the Institutional Animal Care and Use Committee (IACUC) of the University of Indiana School of Medicine (IUSM). The IUSM is accredited by the International Association for Assessment and Accreditation of Laboratory Animal Care. Animals were anesthetized using volatile anesthetics (halothane/isofluorane) and euthanized by decapitation.
Results and Discussion
Proteome-wide analysis of lysine acetylated proteins in cortical astrocytes
As the most abundant type of cell in the mammalian brain, astrocytes carry out a variety of essential functions that include supporting synapse formation, synaptic transmission, and responding to neuronal stress. Astrocytes have been linked to neurodegenerative diseases, such as Alzheimer’s, Parkinson’s, and Huntington’s, as well as amyotrophic lateral sclerosis [42,43]. Efforts to better understand the functions of astrocytes have included recent analyses of the whole-cell proteome and secretome [44–49]. The most recent and comprehensive proteomics study to date was performed using C8-D1A, a type-1 murine astrocyte cell line . Using this cell line, researchers identified 7,183 proteins in the whole-cell astrocyte proteome and 6,067 proteins in the secretome. To date, no one has examined the post-translational modifications (PTMs) that may be decorating the proteome in transformed or dissociated astrocytes.
It has now been established for a wide variety of model organisms that thousands of proteins are subject to lysine acetylation throughout cells; however, an acetylome has yet to be determined for any individual brain cell type to date. To address this knowledge gap, and to further examine whether lysine acetylation is altered in response to infection, we performed proteome-wide analyses of lysine acetylated proteins in cortical astrocytes obtained from rat using a strategy outlined in Fig. 1. The significance of charting the astrocyte proteome is underscored by previous studies suggesting that lysine deacetylase (KDAC) inhibitors, such as suberoylanilide hydroxamic acid and valproic acid, show promise in treating some of the aforementioned neurodegenerative diseases in which astrocytes may play a role [51–53]. We first describe the results of the uninfected astrocytes, with the infected astrocyte acetylome discussed in the following section.
Protein lysates generated from infected and uninfected cortical astrocytes were subjected to proteolysis to generate a peptide suspension. Immunoaffinity purification with anti-acetyl-lysine antibody enriched for acetylated peptides (acetylation mark is denoted by red dot). Identification of acetylated peptides was achieved with mass spectrometry.
Cortical astrocytes were cultured from postnatal day 1–3 Sprague-Dawley rat pups. Generation of whole cell lysates, affinity enrichment of peptides containing acetylated lysine residues, and their identification by mass spectrometry were carried out essentially as described (; see also Materials and Methods). Using this approach, we were able to detect lysine acetyl sites on 516 non-redundant peptides across 304 astrocyte proteins at a false discovery rate (FDR) for peptides of less than 5% (S1 Table). Highlighting the fidelity of our approach and source sample, three marker proteins that distinguish astrocytes from other neuronal cells were detected in our acetylome: fructose-bisphosphate aldolase C (lysine (K) acetylated at amino acid residue 147 (K147)), nuclear factor 1 A-type, NFI-A (K276) and glial fibrillary acidic protein (GFAP) (K258) [50,54,55].
For a global view of the acetylome, we categorized the acetylated astrocyte proteins into functional groups based on gene ontology (S2 Table). The majority of the acetylated sites and proteins cluster into groups including RNA processing/transcription, metabolism, chromatin biology, and translation (Fig. 2A-B). As expected, histone proteins are heavily acetylated (S3 Table). Acetylation and deacetylation of histones by histone acetyltransferases (HATs) or histone deacetylases (HDACs), respectively, are well-established PTMs that modulate gene expression [56,57].
Acetylated proteins (A) and residues (B) were sorted into functional groups based on GO analyses. Lysine acetylation is most prevalent on proteins involved in RNA processing and transcription, metabolism, and chromatin biology. C. The 277 novel acetyl-lysine sites identified in our study are present on proteins that encompass a wide range of cellular functions. D. Proteins detected as lysine-acetylated were grouped based on their respective cellular location.
The first proteomic survey of lysine acetylation was performed in HeLa cells and identified 388 lysine acetylation sites across 195 proteins, which largely clustered into functional categories involving transcription, translation, and metabolism , which is comparable to our astrocyte acetylome. A subsequent study performed on human liver identified 1,047 proteins containing lysine acetyl marks, preferentially on those involved in metabolism . A cardiac acetyl-lysine proteome was determined in guinea pigs, revealing that >60% of acetylated proteins are mitochondrial and mainly involved in metabolism, as well as apoptosis and transcription . It is not surprising that different cell types would have variances in the type and abundance of lysine acetylated proteins, but a striking commonality that emerges from these collective acetylomes is the abundance of acetylation on metabolic proteins.
Of the 304 proteins we detected as being acetylated in astrocytes, 186 are novel, having never been previously reported as acetylated in Rattus novergicus in Uniprot ; thus, our data significantly expands the number of known acetylated lysine sites. The novel lysine acetylation marks we detected were found primarily on proteins associated with RNA processing and transcription, metabolism, chromatin biology, and translation (Fig. 2C).
We examined the presence of acetylated proteins in different subcellular compartments. Our data reveals that proteins subject to lysine acetylation in astrocytes are predominantly found in the nucleus (38.5%), followed by the cytoplasm (28%) and mitochondria (20%). Eleven of the acetylated proteins (3.6% of the acetylome) are secreted from astrocytes (Fig. 2D).
One of the secreted proteins which we identified as acetylated is a complement protein involved in innate immunity: C3, which is acetylated on K215 within the N-terminal beta chain region. Complement C3 has not previously been reported to be acetylated, so it is unclear what the function of this acetylation site may be. It is possible that acetylation of complement C3 could alter folding of the protein and affect secretion and/or cleavage of C3 by C3 convertase, thus regulating the downstream complement cascade. This is particularly intriguing considering our finding that this acetyl mark is increased 3.3-fold upon T. gondii infection (see below), lending support to the idea that acetylation of K215 on C3 may modulate complement function. Treatment with the lysine deacetylase (KDAC) inhibitors sodium butyrate and Trichostatin A has been shown to enhance complement C3 expression and secretion, through increased histone acetylation , but the direct action of KDACs on complement C3 itself was not considered.
Transmembrane glycoprotein NMB precursor (Gpnmb) was another secreted protein on which a novel acetylation site was identified, K170. Also known as osteoclavin in rats, this highly glycosylated protein is anchored in the plasma membrane and has been shown to regulate cellular differentiation, specifically the development of osteoblasts in bone . Gpnmb was also found to be upregulated in both neurons and astrocytes after brain ischaemic injury in rats and overexpression of Gpnmb was protective against ischaemic injury , indicating a role for Gpnmb in neuroprotection. Acetylation of Gpnmb may be involved in the regulation of these neural repair processes, through controlling Gpnmb secretion or glycosylation.
As mentioned above, histone proteins are known to be heavily acetylated and this is no exception in astrocytes (S3 Table). Unexpectedly, we found novel histone acetylation sites on some astrocyte histones. For example, linker histone H1C is acetylated at K17, K75, and K84, in addition to the previously detected K46, K90, and K106 (http://www.phosphosite.org/proteinAction.do?id=3849&showAllSites=true) . The most abundant acetylated non-histone protein in cultured cortical rat astrocytes is α-tubulin, which we detected as acetylated at K40. K40 acetylation is widespread among eukaryotes and its role in the cell remains a topic of intense investigation. Acetylated tubulin is a feature of long-lived, stable microtubules  and may interplay with additional PTMs to form a “tubulin code” that provides microtubules flexibility to execute a wide variety of cellular functions . We performed immunofluorescent analysis using an antibody specific against the acetylated K40 residue of tubulin, which confirms that acetylated microtubules are abundant in cortical astrocytes and localized uniformly throughout the cell (Fig. 3).
The subcellular localization of α-tubulin acetylated at K40 (upper panels) and total α-tubulin (lower panels) through immunofluorescence of isolated cortical astrocytes (green). DAPI staining to highlight nuclei is indicated in blue. Scale bar = 20 μm.
A KEGG pathway analysis using DAVID (Database for Annotation, Visualization and Integrated Discovery; http://david.abcc.ncifcrf.gov/home.jsp) [66,67] of the astrocyte acetylome clustered 123 of the 304 acetylated proteins as proteins linked to neurological disorders, such as Huntington’s disease (18 proteins), Parkinson’s disease (15 proteins), and Alzheimer’s disease (12 proteins) (Table 1 and S4 Table). Acetylated proteins are also associated with canonical metabolic pathways that have been shown to be specifically enriched in astrocytes compared to neurons and oligodendrocytes , including the citrate cycle, fatty acid metabolism, and valine, leucine, and isoleucine degradation (S4 Table).
We also assessed if the amino acids flanking the targeted acetyl-lysine exhibits bias towards a certain motif and if there is significant enrichment or absence of specific amino acids with respect to the general amino acid composition of the entire Rattus norvegicus proteome. For these analyses, we generated WebLogo sequence motifs  and IceLogo heat maps . As previously reported for multiple cell types, we found that lysine acetylation of astrocyte proteins also generally occurs in lysine-rich regions, with a significant enrichment for glycine and alanine at positions-1, -2, and-3 and positions +1, +2, and +5 for alanine only, as shown in Fig. 4A [27,28]. The heat map also shows an absence of serine, proline, and histidine at position +1, and a general lack of leucine in the vicinity of the acetylation site. However, the preponderance of histone proteins in acetylome datasets likely biases the global motif analysis; searching for only the acetylated proteins in mitochondria reveals a striking deviance from other acetylation sites (Fig. 4B). In the acetylated proteins in astrocyte mitochondria, there is a modest enrichment for glutamate (E) at the-1 position, which has been observed before in multiple studies . Analysis of the histone proteins alone revealed a high conservation of the GK motif, with additional lysines at the + and—4 positions, consistent with acetylated histones in other species and cell types [28,58]. Our data lends support to the idea that different motifs are targeted by distinct KATs residing in that particular cellular compartment .
A. Heat map of amino acid composition of acetylation sites in Rattus norvegicus astrocytes, displaying amino acids that are significantly enriched (green) or absent (red) relative to the general amino acid composition of the Rattus norvegicus proteome. B. Sequence motifs of astrocyte acetylation sites +/-7 amino acids from the targeted lysine residue. Motifs were compiled using all acetylated peptides or only those found in histones, non-histone proteins, or mitochondrial proteins.
Differences in lysine acetylation in astrocytes infected with T. gondii
The results above establish that many other proteins in addition to histones are subject to lysine acetylated in astrocytes. We also addressed how the acetylome in cortical astrocytes is impacted by T. gondii infection. In parallel studies, we performed an acetylome on astrocytes that were infected with RH strain T. gondii tachyzoites for 10 hours, at which point vacuoles contain 2–4 parasites. Compared to the uninfected astrocytes, 58 proteins were differentially acetylated when infected with T. gondii. Table 2 lists the 34 proteins that exhibit at least a 2-fold increase in lysine acetylation; 9 of these proteins contain 10 novel acetylated lysines that have yet to be reported.
Notably, several of the acetylated proteins in the infected astrocytes are transcription factors and KATs themselves, which may be involved with the profound changes in gene expression programs previously observed in other T. gondii-infected cells. Consistent with the up-regulation of host cell genes upon T. gondii invasion, hyperacetylation of canonical histones is observed, particularly on H3.3, which shows a remarkable 600-fold increase in lysine acetylation (Table 2). Such a large effect on histone acetylation suggests that modulations in host cell gene expression are largely driven at the epigenetic level.
The greatest increase in acetylation in response to T. gondii infection on a non-histone protein occurs on K170 of GPNMB/osteoactivin, a type I transmembrane glycoprotein nonmetastatic melanoma B (136-fold increase, Table 2). GPNMB plays multiple roles, including bone regeneration, tumor growth regulation, and T-cell inactivation [71–73]. In astrocytes, GPNMB is secreted as a neuroprotective factor [62,74]. As GPNMB is also important for tissue repair , this protein may help manage the damage done by the parasite. The role of acetylation on GPNMB is unknown, and there has been no previous link between this protein and T. gondii infection.
Increased acetylation in select proteins during infection may be due in part to acetylation of KATs like CBP and TIP60 themselves, which display a 6.6- and 6.9-fold increase, respectively (Table 2). K327 acetylation, for example, has been linked to maximizing TIP60 enzyme activity . TIP60 has also been shown to interact with and acetylate c-Myc, dramatically enhancing the stability of c-Myc protein . It is tempting to speculate that T. gondii infection modulates these KAT enzymes, which then leads to hyperacetylation of their corresponding substrates.
Two dozen astrocyte proteins exhibited 2-fold less acetylated lysine levels in the acetylome from T. gondii-infected astrocytes (Table 3), and 28 of the acetylated lysines across these 24 proteins have never been reported in Uniprot. Nearly 30% of the decreased acetylation appears on variant histones, suggesting that T. gondii infection of astrocytes is a large driver of changes in host gene expression. Previous studies have shown that T. gondii infection results in decreased acetylation of core histones H3 and H4 located at the promoters of IFNγ-regulated genes . However, no studies have examined the roles of histone variants in host cells infected with T. gondii. Our results suggest that the rewiring of host cell gene expression in response to infection is highly sophisticated and may involve an opposing interplay of acetylation on canonical versus variant histones.
The most dramatic reduction of lysine acetylation was observed for K408 of the heat shock protein Hsp90α, exhibiting a 19.2-fold decrease in the acetylome of infected astrocytes (Table 3). Hsp90α is an essential molecular chaperone that activates multiple client proteins typically in response to cellular stress . Activation of Hsp90α and interactions with its client proteins and co-chaperones are mediated by several PTMs including lysine acetylation . The acetylation of K408 has not been reported to date, but it was detected as ubiquitylated in mice and humans [81,82], making it possible that acetylation here prevents Hsp90α degradation. The dramatic reduction in deacetylation of K408 suggests that Hsp90α may be suppressed in response to T. gondii infection.
Another protein with greatly reduced acetylation (<10-fold) in the infected astrocyte acetylome is isocitrate dehydrogenase (IDH2), at K180. As a supplier of NADPH, IDH2 is a critical component of the mitochondrial antioxidant pathway , and is necessary for the regeneration of reduced glutathione (GSH), the major antioxidant responsible for preventing ROS damage . IDH2 acetylation at K143 results in a 44-fold loss in activity; deacetylation by SIRT3 restores IDH2 activity and protects cells from oxidative stress . It is possible that T. gondii mediates a deacetylation of IDH2 to protect its host cell from oxidative or other stresses associated with managing infection, but further studies are required to determine the role this lysine plays in IDH2 function in both infected and uninfected astrocytes.
Recent studies have implicated important roles for lysine acetylation in other cell types during viral infection that involve interference with host cell KATs , hijacking of host cell acetylome machinery or acetylation of viral proteins [87–91], and other changes in lysine acetylation during infection or in response to viral pathogens [92,93]. Intracellular bacteria also effect host cell lysine acetylation. For example, Salmonella enterica appear to exploit the host TIP60 KAT activity to promote efficient replication inside host cells . Additionally, Salmonella typhimurium infection increases p53 acetylation in intestinal epithelial cells . Our study lends support to the idea that intracellular pathogens modulate host cell proteomes and PTMs such as lysine acetylation. Previously, it has also been shown that host cell proteins can be differentially phosphorylated in response to T. gondii infection .
It is important to note that some of the changes in acetylation detected in the infected astrocytes could potentially be reflective of changes in the abundance of that protein induced by the infection. Future studies are required to elucidate the biological consequences of the changes we detected in the astrocyte acetylome upon infection with T. gondii, as alterations in lysine acetylation status could affect protein localization, function, stability, or interactions .
T. gondii secretes numerous proteins that alter host cell protein phosphorylation [97–99]. T. gondii possesses several KATs and KDACs, but there is no evidence that these are secreted. A more likely mechanism is that T. gondii effector proteins modulate host KATs and KDACs activities that lead to altered acetylation patterns. Our datasets provide a wealth of new information, including 186 novel lysine acetylation sites to add to the cellular inventory of PTMs. These data serve as valuable resources to generate hypotheses about astrocyte physiology and to interrogate how the changes in host cell lysine acetylation may contribute to the effectiveness of T. gondii infection.
S1 Table. Astrocyte acetylome.
S2 Table. Classification of acetylated astrocyte proteins into functional groups.
S3 Table. Acetylated histone proteins in astrocytes.
S4 Table. KEGG pathway enrichment of acetylated astrocyte proteins.
The authors thank Dr. Jeffrey Silva (CST), Dr. Anita Koshy (University of Arizona), and members of the Sullivan lab for helpful discussions.
Conceived and designed the experiments: AB VJ WJS. Performed the experiments: AB ARC. Analyzed the data: AB VJ AH WJS. Contributed reagents/materials/analysis tools: AB ARC AH WJS. Wrote the paper: AB VJ AH WJS.
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