(A) Schematic representing an L3 worm (∼31 hours post-hatching), showing the somatic gonad, VPCs and germline. Worm expressing mCherry-tagged phospholipase C (PLCδ) PH domain in the germline (top), GFP-tagged myosin in the VPCs and somatic gonad (middle), and merge image (bottom). Scale bar = 10 µm. (B) Schematic of the cells of the somatic gonad (middle) and the germline (extremities) surrounded by a continuous basement membrane (red). Worm at the 12 VPC stage expressing myosin::GFP and mCherry::LAM-1 (laminin-1) to mark basement membrane. Scale bar = 10 µm. (C) Maximum intensity projection images of the somatic gonad expressing GFP myosin. Dotted boxes: dividing cells, enlarged to the right; arrows: contractile ring. Scale bars = 10 µm (left); 5 µm (right). (D-E) Average ring closure speed and asymmetry (somatic gonad: teal blue, first VPC division: purple, second: green, and third: orange). Each VPC stage n(cells) ≥17, n(worms) ≥6. Gonad cells; n(cells) = 29, n(worms) = 12. Error bars = SEM. **: p = 0.0023, unpaired t-test, gonad cells versus 6 VPC stage. *: p = 0.06, ***: p<0.0001, n.s.: p>0.1, unpaired t-test.
Citation: The PLOS ONE Staff (2014) Correction: Quantitative Analysis of Cytokinesis In Situ during C. elegans Postembryonic Development. PLoS ONE 9(12): e116240. https://doi.org/10.1371/journal.pone.0116240
Published: December 17, 2014
Copyright: © 2014 The PLOS ONE Staff. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.