Modules can be identified by segments of megaproteins running from a KS (red) to an ACP (green). Putative protein binding sites are shown as red and purple discs (in the replication origin, oriV) and green discs (‘IR’ for inverted repeat, associated with the biosynthetic cluster promoter regions and likely to be transcriptional regulator binding sites). Like the mupirocin biosynthetic genes the thiomarinol synthases belong to the trans-AT synthases that encode a separate Acyl Transferase while linked to each KS domain is an adjacent “docking domain” consisting of incomplete motifs from Acyl Transferases  that may facilitate or regulate acyl transfer .
Lines connecting orfs are simply to help identify equivalent genes and do not indicate the degree of relatedness. A full map of pTML1 is shown in Figure 2. macpE (labelled “e”), which is critically missing from the thiomarinol cluster, lies between mmpF and mupT.
In Table S1, the gene names at coordinates 78712..79242 and 79587..80669 are incorrectly switched. The gene name at coordinate 78712..79242 should be tmlY, and the gene name at coordinate 79587..80669 should be tmuA. Please view the correct Table S1 here.
Citation: The PLOS ONE Staff (2014) Correction: A Natural Plasmid Uniquely Encodes Two Biosynthetic Pathways Creating a Potent Anti-MRSA Antibiotic. PLoS ONE 9(12): e116036. https://doi.org/10.1371/journal.pone.0116036
Published: December 15, 2014
Copyright: © 2014 The PLOS ONE Staff. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.