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Asynchronous Changes in Vegetation, Runoff and Erosion in the Nile River Watershed during the Holocene

  • Cécile L. Blanchet ,

    cblanchet@geomar.de.

    Affiliations GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany, Department of Marine Organic Biogeochemistry, NIOZ-Royal Netherlands Institute for Sea Research, ‘t Horntje (Texel), The Netherlands

  • Martin Frank,

    Affiliation GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany

  • Stefan Schouten

    Affiliation Department of Marine Organic Biogeochemistry, NIOZ-Royal Netherlands Institute for Sea Research, ‘t Horntje (Texel), The Netherlands

Asynchronous Changes in Vegetation, Runoff and Erosion in the Nile River Watershed during the Holocene

  • Cécile L. Blanchet, 
  • Martin Frank, 
  • Stefan Schouten
PLOS
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Abstract

The termination of the African Humid Period in northeastern Africa during the early Holocene was marked by the southward migration of the rain belt and the disappearance of the Green Sahara. This interval of drastic environmental changes was also marked by the initiation of food production by North African hunter-gatherer populations and thus provides critical information on human-environment relationships. However, existing records of regional climatic and environmental changes exhibit large differences in timing and modes of the wet/dry transition at the end of the African Humid Period. Here we present independent records of changes in river runoff, vegetation and erosion in the Nile River watershed during the Holocene obtained from a unique sedimentary sequence on the Nile River fan using organic and inorganic proxy data. This high-resolution reconstruction allows to examine the phase relationship between the changes of these three parameters and provides a detailed picture of the environmental conditions during the Paleolithic/Neolithic transition. The data show that river runoff decreased gradually during the wet/arid transition at the end of the AHP whereas rapid shifts of vegetation and erosion occurred earlier between 8.7 and ∼6 ka BP. These asynchronous changes are compared to other regional records and provide new insights into the threshold responses of the environment to climatic changes. Our record demonstrates that the degradation of the environment in northeastern Africa was more abrupt and occurred earlier than previously thought and may have accelerated the process of domestication in order to secure sustainable food resources for the Neolithic African populations.

Introduction

The Sahara Desert is presently one of the most arid regions on Earth, but also experienced some of the most drastic environmental changes of the past 10,000 years. During the African Humid Period (AHP, ∼10,000 to ∼6,000 calendar years before present, hereafter referred to as ka BP), monsoonal rains reached areas of the Sahara Desert much further north than today and permitted the development of grasslands and water bodies that hosted large herds of wild game [1]. Human populations thrived in this fertile environment and occupied a large part of the nowadays hyper-arid desert [2]. Due to decreasing summer insolation in the intertropical zone (i.e., between 20°S and 20°N), the rain belt and the vegetation receded to the South and forced the human populations to leave the Sahara at ∼6.5 ka BP [2], [3]. It was during this period of large environmental changes that major societal reorganizations occurred, such as the implementation of pastoralism and agriculture into human lives, as well as the development of collective rituals and religious beliefs [3], [4]. Uncontroversial archeological evidence demonstrates the presence of domesticated cattle in human settlements from ∼8 ka BP onwards in northern Egypt (Nabta) [5][7]. There is an ongoing debate as to whether North Africa has been a center of domestication or whether the domesticated cattle found there originated from the Near East [7]. In any case, North Africa played a decisive role as a focal center and a corridor for the dispersal of domesticates throughout the African continent [6]. Furthermore, the initiation of food production in NE Africa occurred with a delay of about 2000 years as compared to the Fertile Crescent of the Near East and exhibited a very distinctive pattern. Archeological evidence in NE Africa suggests that animal domestication occurred without agriculture but within mobile hunter-gatherer groups and post-dated the development of pottery [8]. The role of the major local environmental changes and their influence on these different models of food production has not yet been clearly determined.

The environmental degradation at the end of the AHP has been considered an important factor for the introduction of domesticates in Neolithic populations [4]. However, the exact timing of the wet-dry transition in NE Africa is still debated. A reconstruction of environmental changes obtained from geological and archeological data showed a gradual decline in rainfall and a southward retreat of the vegetation during the Holocene [2]. Such a gradual transition was also observed in the pollen and sedimentological record of Lake Yoa (central Sahara) for the past 6 kyr [9] and in the speleothem record of paleo-rainfall on the Oman Peninsula between 8 and 2 ka BP [10]. This view has recently been challenged by the reconstruction of the wet-dry transition within in a few centuries around 5.5 ka BP from a sediment core off Somalia, similar to records from western Africa [11]. These discrepancies question the exact role of environment-human relationships at the beginning of the Holocene.

Here we present an alternative view on the evolution and the timing of environmental changes during the Holocene by reconstructing conjointly the changes in vegetation, erosion and rainfall dynamics from the same sedimentary archive. This allows the establishment of a precise timeframe for the different processes in order to understand their causal relationships and provides a detailed environmental context for human evolution in North Africa. We used sediment core P362/2-33 that was retrieved from the Nile deep-sea fan at 700 m water depth [12], [13] (Fig. 1). The unique feature of this 6-m long core is a 5-m thick section of finely laminated sediments, which were deposited during the AHP and which are a local expression of sapropel S1 [13]. These sediments offer a very high temporal resolution of several mm/year and provide an integrated record of past environmental changes within the Nile watershed. During the AHP, the drainage area of the Nile was significantly larger than today due to the contribution from lake and river systems located in the present-day Sahara Desert [1], [14]. The fluvial input of particulate and dissolved matter that has been stored in sediments on the Nile deep-sea fan is thus a unique source of information on past environmental changes in NE Africa.

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Figure 1. Map of the present-day land cover in northern Africa.

A: Location and averaged radiogenic isotope composition of the three main sources of sediments to the Nile deep-sea fan [44]. B: Types of vegetation and estimated percentage of trees at present in Northern Africa [58]. The gray color represents the Sahara Desert, which is presently non-vegetated. The course of the Nile River is represented by the dark blue line. The location of core P362/2-33 is indicated by the red star. The present-day northern reach of the summer and winter African Rain Belt (ARB) are depicted as red and blue dashed lines, respectively.

http://dx.doi.org/10.1371/journal.pone.0115958.g001

Material and Methods

Marine sediment core P362-2/33 (700 m water-depth, 31°40.51N; 29°45.00E) was retrieved during the project “West Nile Delta” funded by RWE-Dea but not specifically for the purpose of the present study. The cruise permit 201–2008 was issued on the 21.01.2008 by the Egyptian Ministry of Defense (Authority of Armed Forces Operations, through the Naval Forces based at Alexandria Naval Base) for RWE-Dea. The core location is in public area. No human or vertebrate animals were used during this study.

The sedimentation on the Nile deep-sea fan is largely controlled by the seasonal discharge of the Nile River (especially at the location of core P362/2-33, which is in the vicinity of the most active Rosetta Canyon) [12]. Precipitation patterns at the source of the Nile River largely determine sediment provenance: While the White Nile and Sobat River contribute a minor part of the annual sediment load (3.5%). but provide a quasi-constant flow of water to the Nile, the Blue Nile and Atbara River provide the major part of the annual sediment load (96.5%) mainly during boreal summer (Fig. 1a) [15]. Eolian dust originating from North African sources and hemipelagic sedimentation (diatoms, foraminifera) also contribute to sedimentation at the core location during the spring and winter months [13].

Description and age control of sediment core P362/2-33

A detailed description of core P362/2-33 and the age model were published previously in ref. [13]. The upper 70 cm of the 6-m long sediment core consist of brownish to greyish bioturbated sediments, below which faint laminations are observed between 75 and ∼105 cm core depth. Well-preserved millimeter-scale laminations are observed below 140 cm core depth and most probably result from deposition of highly dense, suspension-rich (hyperpycnal) flows formed by particle-laden seasonal Nile flood plumes in the water column [16]. The age model of the sediment core was based on fourteen 14C ages of fossil planktonic foraminifera shells, measured at the Leibniz-Laboratory for Radiometric Dating and Isotope Research (University of Kiel). Correction of reservoir ages and calibration of the 14C ages are described in ref. [13]. The core covers most of the Holocene, with the bottom of the core being dated at ∼9000 14C yrs BP (9.5 ka BP) and the uppermost sediment sections were deposited prior to 1800AD. Sedimentation rates vary from ∼650 cm/kyr in the lower part of the core to ∼8 cm/kyr in the upper part.

Stable oxygen isotope of foraminifera

Stable oxygen isotopes (δ18OC) were measured every 3 cm on the planktonic foraminifera G. ruber (white and >250 µm) at the GEOMAR (n = 176) (S1 Table). The sediment samples were washed and dry-sieved and ∼20 specimens were picked, crushed and gently cleaned with ultrapure (Milli-Q) water. The shells were dissolved in orthophosphoric acid at 70°C in a Kiel II carbonate device and the CO2 released was analyzed by a Thermo Scientific MAT 252 isotope ratio mass spectrometer (IRMS). The 18O/16O ratios are reported in ‰ relative to the Vienna-Pee Dee Belemnite (VPBD) standard, where: δ18O = [((18O/16O)sample/(18O/16O)VPDB)-1]*1000. Analytical precision of <0.06 ‰ for the δ18O measurements was determined from repeated analyses of a Solnhofen limestone which is calibrated against NBS19 as internal standard.

Radiogenic neodymium and strontium isotope signature of detrital sediments

The methodology used for extracting neodymium (Nd) and strontium (Sr) signatures from detrital sediments is similar to that described in ref. [13]. However, the set of samples published in ref. [13] (a few grain-size fractions) is very different to the one we present here (bulk sediment measurements for the whole core), which is unpublished. The bulk sediment samples (∼3 g) were first decarbonated (using a buffered acetic acid solution, pH ∼4.5), then leached to remove any authigenic ferro-manganese coatings [17] (using a buffered hydroxylamine hydrochloride solution, pH ∼3.9) and finally ∼0.05 g of the homogenized detrital sediment was totally dissolved (using Aqua Regia, HNO3 conc. and HF conc.) (S2 Table). The samples were purified and separated using standard ion-chromatography procedures [18], [19]. The Nd and Sr isotope compositions were measured on a Nu Instruments multi-collector inductively-coupled plasma mass spectrometer at GEOMAR. Blank concentrations were negligible for isotopic analyses (<0.3 ng for Nd and <3,4 ng for Sr).

External reproducibility (2σ) was first estimated by repeated measurements of in-house SPC and SPEX standards for Nd and the AA standard for Srranging from ±0.14 to ±0.63 εNd units (±14–63 ppm, n = 57) for Nd and from ±0.000009 to ±0.000048 (±12–68 ppm, n = 58) for Sr. External reproducibility was further assessed by repeated measurements of the JNdi standard for Nd isotopes and the NBS SRM 987 standard for Sr isotopes, and yielded 2σ uncertainties of ±0.3 εNd units (±30 ppm, n = 57) and ±0.00003 (±42 ppm, n = 58) for Sr. The isotope results reported were normalized to the accepted values of the JNdi standard for Nd (143Nd/144Nd = 0.512115) and of the NBS SRM 987 standard for Sr (86Sr/87Sr = 0.710245). The Nd isotope ratios are reported as εNd: [20].

Alkenones, stable carbon isotopes of n-alkanes and BIT index

The lipids were extracted from 43 sediment samples with a DIONEX Accelerated Solvent Extractor 200 at the NIOZ using a solvent mixture of 9∶1 (v/v) dichloromethane (DCM)/methanol (MeOH). After the addition of internal standards C22 anti-isoalkane (n-alkanes), 10-nonadecanone (alkenones) and C46 glycerol trialkyl glycerol tetraether (GDGTs), the total lipid extract was separated into apolar, ketone and polar fractions using pipette column chromatography loaded with aluminum oxide and the solvent mixtures 9∶1 (v/v) hexane/DCM, 1∶1 hexane/DCM and 1∶1 DCM/MeOH as eluents, respectively. The apolar fraction was then separated into saturated hydrocarbon (long-chain odd n-alkanes and the C22 anti-iso standard) and aromatic fractions using pipette columns loaded with Ag+-impregnated silica and hexane and ethylacetate as eluents, respectively.

Molecular identification of the alkenones and n-alkanes was performed on a Thermo Finnigan Trace Gas Chromatograph (GC) Ultra coupled to a Thermo Finnigan DSQ mass spectrometer (MS). The alkenones and the n-alkanes were quantified using HP 6890 GCs with CP Sil-5 columns (50 m for the alkenones and 25 m for the n-alkanes) and helium as the carrier gas. The Uk37′ index [21], defined as C37∶2/(C37∶2+C37∶3), was used to estimate surface seawater temperatures (SSTs) for 39 samples run in duplicate (mean reproducibility of ±0.8°C) (S4 Table and S1b Fig.), following the equation [22], [23]: SST = −0.957+54.293(Uk37′)−52.894(Uk37′)2+28.321(Uk37′)3.

The stable carbon isotope compositions of the long-chain odd n-alkanes were measured for 41 samples (in duplicate or triplicate) on an Agilent 6800 GC coupled to a ThermoFisher Delta V Isotope Ratio MS (S3 Table and S2 Fig.). Isotope values were measured against calibrated external reference gas and the performance was checked daily by injection of two calibrated n-C20 and n-C24 perdeuterated n-alkane standards. The 13C/12C isotope ratios of n-alkanes are reported in the standard delta notation (δ13C) in ‰ against the V-PDB standard: δ13C = [((13C/12C)sample/(13C/12C)VPDB)-1]*1000. The average reproducibility is 0.47‰ for the n-C27 n-alkane, 0.32‰ for n-C29, 0.29‰ for n-C31 and 0.49‰ for n-C33. The average reproducibility of the internal C22 anti-iso standard was 0.9‰ (n = 62) and the reproducibility of an external n-C24 standard was 0.46% (n = 31). A weighted average was calculated using the relative proportion (area under the peak divided by the sum of the areas of n-C27, n-C29, n-C31 and n-C33) and isotope ratio of each n-alkane (S2 Fig.). The estimation of the % fraction of C4 plants (%C4) was realized using a two end-members mixing equation [24]: %C4 = 100-(−7.4627* δ13Cwax−160.82). The terrestrial provenance of the n-alkanes was assessed by calculating the carbon preference index (CPI), which is the ratio between odd and even n-alkanes [25]. Long-chain odd n-alkanes originate from terrestrial higher plants with a CPI of >3, whereas long-chain even n-alkanes originate from petroleum sources with a CPI of ∼1. The CPI in core P362/2-33 ranges from 3 to 9 with a mean value of 6.6, which indicates the terrestrial origin of the n-alkanes throughout the record.

The polar fraction of 41 samples (S4 Table), containing the GDGTs, was dissolved in a mixture of 99∶1 (v/v) hexane/propanol and filtered through 0.45 mm PFTE filters. GDGTs were analyzed (in triplicate) by high performance liquid chromatography (HPLC)/MS in single ion monitoring mode on an Agilent 1100 series LC/MSD SL [26], [27]. The Branched and Isoprenoid Tetraether (BIT) index was calculated following [28]: BIT =  (GDGT-I+GDGT-II+GDGT-III)/(GDGT-I+GDGT-II+GDGT-III+Crenarchaeol), whereby the GDGTs refer to structures shown in S3 Fig. The averaged standard deviation for the calculated BIT index is ±0.01.

Results and interpretations

The strength of river runoff is reconstructed from stable oxygen isotope compositions of surface seawater obtained from planktonic foraminifera (δ18OSW) (Fig. 2a). The δ18OSW record was obtained by correcting the measured δ 8O signature of planktonic foraminifera for changes in surface seawater temperature (SST) determined using the Uk37 paleothermometer (S1 Fig.). The SSTs reconstructed for core P362/2-33 fall in the same range as those previously reconstructed in the Levantine Basin (16 to 26°C) (S1b Fig.) [29], [30]. To estimate the δ18OSW, we used the paleotemperature equation established for Orbulina universa [31] and applied a correction for changes in the ice volume [32]. The δ18OSW values obtained for Late Holocene sediments are similar to the present-day eastern Mediterranean seawater δ18O [33] (S1c Fig.) and the values for the Holocene fall in a similar range and follow a trend similar to those previously obtained in the Levantine Basin (∼2 to −1‰) [29]. This demonstrates that the reconstructed δ18OSW is not biased by the fact that foraminifera and coccolithophores bloom during different seasons (summer and spring, respectively) and therefore potentially recorded different SSTs (see discussion in ref. [29]). The δ18OSW has primarily been related to the changes in surface seawater salinity (SSS) changes, which has been influenced by the precipitation/evaporation balance and by the changes in river runoff (amount effect) [34]. However, as recently shown [35], the δ18OSW/SSS relationship may have varied during the Holocene due to changes in the δ18O of freshwater end-member (i.e., the precipitation and the Nile River water), resulting from changes in the hydrological cycle or in the provenance of the river water. In our case, these various effects cannot be deciphered but they all have the same effect on the δ18OSW: a decrease in δ18OSW can be interpreted as a decrease in salinity (either due to a higher precipitation/evaporation ratio or increased runoff), and/or as an increase in δ18O of the freshwater end-member (either due to a more intense convection or a dominant Blue Nile source for the river water [36], which both led to higher runoff). During the early Holocene, δ18OSW values around 0‰ were closer to the present-day δ18O signature of Nile River water (0.8 to −0.6‰, ref. [36]) and might therefore reflect enhanced river runoff leading to lower salinities (S1c Fig.). During the early Holocene, the δ18OSW exhibited large amplitude and high frequency variations, which might be related to a large variability of the Nile runoff during the so-called ‘Wild Nile’ period (Fig. 2a). The geomorphological record of this time interval has demonstrated the occurrence of very intense floods with Nile river levels up to 5 m higher than at present [15], [37]. This is also reflected by very high sedimentation rates and the deposition of mm-thick layers of flood-derived sediments in our record (Fig. 2f) [13].

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Figure 2. Changes in precipitation, vegetation and erosion dynamics in the Nile watershed during the Holocene.

A: Summer (June-August) insolation at 20°N [59] (dashed line) and oxygen isotope signature of the surface seawater (δ18OSW) at the location of our core, which reflects changes in sea-surface salinity and river runoff (S1 Fig.) and has been controlled by orbitally-induced changes in precipitation. B:. Paleo-precipitation records obtained from a speleothem on the Oman Peninsula (δ18O), rainfall regime of which has been under influence of the Indian monsoon system [10] and obtained from marine sediments off the coast of Somalia (δD of n-alkanes), the rainfall regimes of which has been under the influence of deep-convection in the Indian Ocean [11]. C: Stable carbon isotope composition of higher-plant n-alkanes reflecting the proportion of C4 (mainly grasses) versus C3 plants (trees/shrubs). D: BIT index recording changes in the relative contribution of soil organic matter input. E: Radiogenic Sr and Nd isotope signatures of the bulk detrital fraction of the sediments documenting changes in sediment provenance. F: Sedimentation rates. Radiocarbon dates are indicated at the bottom of the panel as red triangles and black rectangles at the bottom of the figure indicate the laminated parts of the core (see “Material and Methods” section and ref. [13]).

http://dx.doi.org/10.1371/journal.pone.0115958.g002

The close similarity in long-term trends between our δ18OSW record and the speleothem δ18O record of paleo-precipitation on the Oman Peninsula suggest that river runoff responded quasi-linearly to changes in rainfall intensity in the Nile watershed (Fig. 2b) [10]. The speleothem record of Quunf Cave on the Oman Peninsula exhibits a gradual decline in precipitation throughout the Holocene that followed the changes in summer insolation, which is also observed in our δ18OSW record. This implies that non-linear and threshold mechanisms (such as lake overflow) played a limited role in controlling freshwater discharge. Recently, another paleoprecipitation record was obtained from δD analyses of n-alkanes in marine sediments retrieved off the coast of Somalia/Ethiopia that challenged the prevailing consensus of a gradual wet/dry transition at the end of the AHP in Eastern Africa [11]. Contrary to the speleothem record from the Oman Peninsula and other regional records [2], [9], [10], this δD record exhibits a rapid decrease in rainfall/humdity between ∼6 and 4 ka BP (Fig. 2b). The authors infer that such a rapid termination of the AHP (which was also observed in other records from eastern African lakes [11]) was due to the fact that the hydroclimate regime on the Horn of Africa has mostly been under the influence of deep convection and sea surface temperatures in the western Indian Ocean.

New environmental information on the evolution of vegetation cover, soil dynamics and erosion patterns has been generated from sediment core P362/2-33. Changes in vegetation cover are reconstructed using the stable carbon isotope composition of long-chain odd n-alkanes, which originate from higher plant leaf waxes (δ13Cwax) (Fig. 2c). Plants with C4 and C3 photosynthetic pathways (essentially corresponding to warm-season grasses/sedges and cool-season grasses/trees/shrubs, respectively) produce leaf waxes with different δ13C values that are preserved during transport and sedimentation, and particularly during oxidative diagenesis [38][40]. Here we use the weighted average of δ13C of four n-alkanes (n-C27, n-C29, n-C31 and n-C33) to estimate changes in the contribution of plants using a C4 metabolic pathway to the n-alkane pool (%C4) in the drainage basin (Fig. 2c). Due to their CO2-concentrating mechanism, C4 plants (such as warm-season grasses and sedges) are generally isotopically enriched in 13C compared to C3 plants, which include most trees, cool-season grasses, and sedges [40]. The δ13C records measured on each n-alkanes have similar long-term trends, with higher values (reaching −21‰ for n-C33) between 9.5 and 8 ka BP and lower values (down to −30‰ for n-C31) between 6 and 0 ka BP (S2 Fig.). The estimation of the % fraction of C4 plants (% C4) was realized using the 2 end-members mixing equation from ref. [24] and the terrestrial provenance of the n-alkanes was assessed by calculating the Carbon Preference Index (see “Material and Methods”). The n-alkanes can be transported by wind or rivers but it is assumed here that they have been mainly transported to the core site by the Nile River because the concentration and accumulation rates of n-alkanes show a long-term trend similar to that of the branched GDGTs, which are transported only by rivers (S3a,b Fig.) [28]. Furthermore, Blanchet et al. [13] have shown that at core site P362/2-33, the terrigenous sedimentation has been largely controlled by fluvial inputs and that eolian inputs were significant only around 3–4 ka BP, when the amount of n-alkanes was low in the sediments (S3a Fig.). The δ13Cwax varied by 5‰ in our record, which corresponds to proportional abundances of C4 plants between ∼80 and 40% (Fig. 2c), although these numbers have to be interpreted with some care due to the widely varying concentrations of n-alkanes in C3 and C4 plants [41]. The prevalence of C4 grasses during the AHP reflects the northward migration of the African Rain Belt that led to the expansion of C4-dominated savannah-type vegetation into areas of the Sahara that are not vegetated today (Fig. 1b) [42], [43]. A rapid stepwise decrease in δ13Cwax between 8.5 and ∼7.8 ka BP and between 6.5 and 6 ka BP (from −24 to −28‰) documents a drastic reduction in C4 plant cover, which was a consequence of the retreat of the vegetation in the Sahara during the southward migration of the African Rain Belt at the end of the AHP.

Another prominent feature of our record is the abrupt switch in sediment provenance accompanied by a decrease in soil organic matter input and erosional activity. The amount of soil organic matter input to the sediments was estimated using the Branched and Isoprenoid Tetraethers (BIT) index (Fig. 2d), which is the ratio between the contents in terrigenous branched GDGTs and marine crenarchaeol [28]. Castañeda et al. [30] showed that the BIT index can be strongly influenced by the production of marine crenarchaeol and therefore advised to compare the contents and accumulation rates of branched GDGTs and crenarchaeol to the BIT index. At the site of core P362/2-33, the BIT index has obviously been affected by changes in the soil organic matter content rather than by the changes in crenarchaeol content (S3b,c,d Fig.), which suggests that the changes in the BIT index most likely reflect changes in soil formation and erosion. A marked decrease in soil organic matter input is documented by the BIT index from 0.7 to 0.2–0.3 between ∼9 and 7.3 ka BP (Fig. 2d). This decrease in soil organic matter input is nearly synchronous with a switch in sediment provenance recorded in the radiogenic Nd and Sr isotope signatures (εNd and 87Sr/86Sr) of the detrital sediment fraction (Fig. 2e). As shown on Fig. 1a, the sources of the Nile River are characterized by specific εNd and 87Sr/86Sr signatures depending on their lithology [44]. At 8.7 ka BP, the εNd and 87Sr/86Sr of the detrital sediment fraction recorded an abrupt shift from more basaltic (εNd ∼−3 and 87Sr/86Sr ∼0.707) to more granitic (εNd ∼−4 and 87Sr/86Sr ∼0.71) signatures. This documents a decrease in the proportion of sediments originating from the Blue Nile (Ethiopian Highlands) and an increased supply of sediments from the White Nile/Sahara regions (Fig. 2e). This enhanced contribution from the White Nile/Sahara regions was already reported in core P362-2/33 based on the grain-size distribution of the terrigenous fraction (i.e., changes in the proportion of grain-size end-members) [13]. This switch in sediment provenance was accompanied by a drop in sedimentation rate from 6 mm/yr to less than 2 mm/yr between 9 and 8.7 ka BP (Fig. 2f) and implies that the massive erosion of Blue Nile soils due to intense flooding activity decreased abruptly at 8.7 ka BP and thus preceded the rapid degradation of the green Sahara.

Discussion

These drastic modifications of the environment during the early Holocene reconstructed from the proxy-record of core P362/2-33 have important implications. Firstly, the proxy-records of the termination of the AHP in NE Africa show considerable spatial and temporal variability. While some records have depicted a gradual and sometimes stepwise desiccation following the decrease in summer insolation [9], [45], [46], others indicate a rapid desiccation around 5.5 ka BP that occurred significantly faster than the change in orbital forcing [11], [47]. Our record supports an alternative scenario in which a rapid degradation of the environment occurred between 8.7 and 6 ka BP, significantly earlier than previously inferred from eastern Saharan records (Fig. 2). It must also be noted that this environmental degradation occurred while water discharge in the Nile drainage basin and precipitation on the Oman Peninsula [10] decreased gradually and did not exhibit a rapid wet/dry transition between 4 and 6 ka like on the Horn of Africa [11]. This non-linear response of the environment to changes in river discharge (precipitation) might be explained by feedback processes or threshold mechanisms between vegetation and precipitation [48], [49]. A recent study highlighted the potential role of differential responses of distinct components of the vegetation system during a climatic transition [50]. Depending on their sensitivity to environmental constraints, various types of plants exhibit distinct behavior during a climatic transition, but the presence a large diversity of plant types (including more resilient plants) might help to stabilize the environment and generate a gradual change. The pollen record of a gradual transition of vegetation during the AHP termination at Lake Yoa might reflect such a process [9]. In contrast, our record of an early abrupt retreat in grass cover might reflect the high sensitivity of C4 plants to small changes in precipitation. A modeling study described the process by which the vegetation system degraded quickly (faster than the forcing) after reaching a precipitation threshold, causing the switch to the other stable equilibrium condition, the desert state [49]. If this interpretation were correct, it would imply that large-scale vegetation changes might occur in the absence of strong precipitation-vegetation feedback processes (i.e., the degradation of the vegetation did not provoke a further decrease in precipitation) contrarily to what was proposed in earlier studies [48].

An early arid event such as that in our record at ∼8.7 ka BP was previously identified in several records in NE Africa around 8.6–8.2 ka BP as a transient episode that sometimes marked the initiation of the AHP termination [45][47], [51]. In our record, however, this event marked a permanent modification of erosion dynamics and vegetation cover. It was claimed previously that arid events in North Africa resulted from teleconnections to cooling events in the Atlantic (such as the 8.2 ka event) [3] given that they occurred during periods of low sea-surface temperatures in the Mediterranean [52] and the tropical Atlantic Ocean [53]. However, our record shows that the onset of desiccation in the Nile watershed preceded the cooling event by at least 500 years, which suggests that arid events in North Africa may as well have resulted from internal destabilization of the climate-vegetation system, which in turn may have influenced the climate at higher latitudes [46], [54].

Another important implication of our record is the establishment of a detailed environmental context for the major reorganizations within the human populations in the Sahara Desert. The transition between the Paleolithic and the Neolithic is marked by the beginning of food production by the human populations and coincided with the drastic modification of the environmental conditions in NE Africa. The beginning of food production occurred through animal domestication in the form of mobile herding [6], [8] but the origin of the domesticated cattle, whether it was introduced from the Near East or originated from a local bovid source remains an unresolved question [5], [7]. In any case, northern Africa has played a decisive role as a focal center and a corridor for the dispersal of domesticates throughout the African continent [6].

Uncontroversial archeological remains date the first domesticated cattle at around 8.5–7.5 ka BP in the Nabta-Bir Kiseiba region [5][7] immediately following the significant environmental change that occurred in the Nile watershed at 8.7 ka in our record. This was characterized by a switch in sediment provenance towards higher contributions by the White Nile and Saharan areas [13] (Fig. 3b) and by a retreat of the savannah with lowest grass content attained between 7 and 6 ka BP (Fig. 3a). Human populations were thus probably forced to adapt to the rapid degradation of the environment by relying increasingly on domesticated animals and by migrating to areas allowing sustainable herding, which supports the hypothesis that human-environment relationships played a significant role in the domestication process [4], [55].

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Figure 3. Environmental context of major steps of human evolution in NE Africa during the Holocene.

A: Percentage of C4 plants, as estimated from the δ13C of higher-plants n-alkanes (see Fig. 2). B: Sediment source as estimated from the radiogenic Sr isotope signature of the detrital sediment fraction. C: Phases of human evolution, as compiled from ref. [5][8] and [54][56].

http://dx.doi.org/10.1371/journal.pone.0115958.g003

The first evidence of domesticated cattle in NE Africa lags behind the Fertile Crescent occurrence by ∼2000 years [8]. It thus seems that early Neolithic populations in the Fertile Crescent benefited from the favorable conditions during the AHP to implement agriculture and then herding while NE African populations thrived in the Green Sahara and only turned to pastoralism when the living conditions became more difficult [6], [8].

Around 6 ka BP, widespread ritual burial of cattle, known as the ‘African Cattle Complex’ [55], and the first evidence of dairying in the Central Sahara [56] demonstrated that pastoralism had spread in the Sahara and had become a well-established subsistence method. At this time, the savannah had almost entirely disappeared from the Sahara, which became increasingly arid between 6 and 2.5 ka BP, as shown by the radiogenic strontium signature of detrital sediments (Fig. 3b), as well as grain-size data from core P362/2-33 [13]. From 5 ka BP onwards, the population in the Nile Valley increased gradually and gave rise to the Egyptian Pharaonic Kingdoms and to the subsequent development of agriculture [57]. In our record, this period is marked by the most arid conditions as evidenced by the highest amounts of eolian dust found in the sediments between ∼5 and 3 ka BP (Fig. 3b and ref. [13]).

A pronounced shift in sediment provenance towards a Blue Nile source occurred at 2.5 ka BP (Fig. 3a) and reflects the re-establishment of seasonal rainfalls in the Blue Nile drainage basin after 3 ka BP due to an increase in autumn insolation [13], [46], [57]. However, these floods were much less vigorous than those during the Early Holocene (as documented by the lower sedimentation rates, Fig. 2f), because summer insolation that mainly controls monsoon strength at the Blue Nile source was lower during the Late Holocene than during the Early Holocene [13].

Our new continuous high-resolution record of changes in vegetation, erosion and river runoff within the Nile River drainage area allows to place the main steps of Neolithic human evolution into a detailed environmental context. An abrupt degradation of the vegetation and a switch in sediment source during the Early Holocene (at 8.7 ka BP) occurred without a significant decrease in river runoff and precipitation. Our record therefore confirms the large regional heterogeneity of environmental change in NE Africa and provides new insights into the climatic mechanisms involved in the termination of the North African Humid Period, in particular the existence of threshold mechanisms. The degradation of the vegetation probably had a considerable impact on the initiation of cattle domestication in North Africa and confirms the hypothesis that aridification in North Africa had a profound impact on human evolution.

Supporting Information

S1 Fig.

Reconstruction of δ18O of surface seawater. A: δ18O measured on the planktonic foraminifera Globigerinoides ruber. B: Surface seawater temperatures (SST) reconstructed using the alkenone insaturation index (Uk37′). Measured points are indicated by the filled diamonds; values in between measured points were estimated using linear interpolation provided by the Analyseries software package (http://www.lsce.ipsl.fr/Phocea/Page/index.php?id=3) in order to provide the same spatial resolution as the δ18O record. C: δ18O of the surface seawater (δ18OSW), as compared to the present-day values for the Nile freshwater and the eastern Mediterranean surface seawater (0–200 m water depth) [33], [36].

doi:10.1371/journal.pone.0115958.s001

(TIFF)

S2 Fig.

Isotopic composition of long-chain odd n-alkanes. A: Carbon isotope composition (δ13C) of n-C27; B: δ13C of n-C29; C: δ13C of n-C31 and D: δ13C of n-C33. E: Weighted average of the δ13C of n-C27-n-C33.

doi:10.1371/journal.pone.0115958.s002

(TIFF)

S3 Fig.

Content and accumulation rates (AR) of lipid biomarkers. A: AR (dashed orange line) and concentration (thick red line) of long-chain odd n-alkanes. B: AR (dashed green line) and concentration (thick green line) of branched GDGTs, with the structure of the dominant branched GDGTs. C: AR (dashed blue line) and concentration (thick blue line) of crenarchaeol, with its structure. D: BIT index.

doi:10.1371/journal.pone.0115958.s003

(TIFF)

S1 Table.

Surface seawater properties. Stable oxygen isotopes (δ18O) for the planktonic foraminifera Globigerinoides ruber; surface seawater temperature (SST) as evaluated using the alkenone paleothermometer (Uk37′) for 39 samples and linearly interpolated; δ18O of the surface seawater (δ18OSW) obtained using the paleotemperature equation of ref. [31].

doi:10.1371/journal.pone.0115958.s004

(DOC)

S2 Table.

Radiogenic Nd and Sr isotopes for the total dissolutions. The radiogenic Nd and Sr isotopes of the total dissolutions is the signal carried by the siliciclastic (detrital) fraction of the sediments. All Nd and Sr isotope ratios are given with a 2σ external reproducibility.

doi:10.1371/journal.pone.0115958.s005

(DOC)

S3 Table.

Carbon isotope ratios in long-chain odd n-alkanes. The weighted average of the δ13C of the n-C27, n-C29, n-C31 and n-C33 n-alkanes is given with the standard deviation. The percentage of C4 plants was calculated using the mixing model by ref. [24].

doi:10.1371/journal.pone.0115958.s006

(DOC)

S4 Table.

Concentration and accumulation rates of GDGTs and BIT index.

doi:10.1371/journal.pone.0115958.s007

(DOC)

Acknowledgments

We thank the chief scientist of Poseidon cruise P362 (2008) Tomas Feseker and the PI of the West Nile Delta project (funded by RWE-Dea) Warner Brückmann for providing access to core P362/2-33. Kevin Brown is thanked for measuring the δ18O values of the foraminifera, and we acknowledge the analytical support at GEOMAR and at NIOZ. All data presented in this article have been deposited in the PANGAEA database (http://doi.pangaea.de/10.1594/PANGAEA.828054). The comments of three reviewers (including Germain Bayon) greatly helped to improve the present manuscript, which also benefited from fruitful discussions with Rik Tjallingii, Nick Marriner and Guillaume Leduc.

Author Contributions

Conceived and designed the experiments: CLB MF SS. Performed the experiments: CLB. Analyzed the data: CLB MF SS. Contributed reagents/materials/analysis tools: CLB MF SS. Wrote the paper: CLB MF SS.

References

  1. 1. Drake NA, Blench RM, Armitage SJ, Bristow CS, White KH (2011) Ancient watercourses and biogeography of the Sahara explain the peopling of the desert. Proc. Nat. Acad. Science USA 108:458–462. doi: 10.1073/pnas.1012231108
  2. 2. Kuper R, Kröpelin S (2006) Climate-Controlled Holocene Occupation in the Sahara: Motor of Africa's Evolution. Science 313:803–807. doi: 10.1126/science.1130989
  3. 3. Brooks N (2006) Cultural responses to aridity in the Middle Holocene and increased social complexity. Quat. Int. 151:29–49. doi: 10.1016/j.quaint.2006.01.013
  4. 4. Hassan FA (2002) Paleoclimate, food and cultural change in Africa: an overview. In Hassan FA, editor. Droughts, Food and Culture: Ecological Change and Food Security in Africa's Later Prehistory. New York: Kluwer Academic/Plenum Publishers pp.11–26.
  5. 5. Gifford-Gonzalez D, Hanotte O (2011) Domesticating animals in Africa: Implication of genetics and archeological findings. J. World Prehist. 24:1–23. doi: 10.1007/s10963-010-9042-2
  6. 6. Linseele V (2013) Early stock keeping in northeastern Africa: Near Eastern influences and local developments. In Shirai N, editor. Neolithisation of Northeastern Africa. Studies in Early Near Eastern Production, Subsistence and Environments. Berlin: Ex Oriente pp. 97–108.
  7. 7. Stock F, Gifford-Gonzalez D (2013) Genetics and African cattle domestication. Afr. Archaeol. Rev. 30:51–72. doi: 10.1007/s10437-013-9131-6
  8. 8. Garcea EAA (2004) An alternative way towards food production: The perspective from the Lybian Sahara. J. World Prehist. 18 (2):107–154. doi: 10.1007/s10963-004-2878-6
  9. 9. Kroepelin S, Verschuren D, Lézine AM, Eggermont H, Cocquyt C, et al. (2008) Climate-driven ecosystem succession in the Sahara: The past 6000 years. Science 320:765–768. doi: 10.1126/science.1154913
  10. 10. Fleitmann D, Burns SJ, Mudelsee M, Neff U, Kramers J, et al. (2003) Holocene forcing of the Indian Monsoon recorded in a Stalagmite from Southern Oman. Science 300:1737–1739. doi: 10.1126/science.1083130
  11. 11. Tierney J, deMenocal P (2013) Abrupt shifts in Horn of Africa hydroclimate since the Last Glacial Maximum. Science 342:843–846. doi: 10.1126/science.1240411
  12. 12. Feseker T, Brown KR, Blanchet C, Scholz F, Nuzzo M, et al. (2010) Active mud volcanoes on the upper slope of the western Nile deep-sea fan—first results from the P362/2 cruise of R/V Poseidon. Geo-Mar. Lett. 30:169–186. doi: 10.1007/s00367-010-0192-0
  13. 13. Blanchet CL, Tjallingii R, Frank M, Lorenzen J, Reitz A, et al. (2013) High- and low-latitude forcing of the Nile River regime during the Holocene inferred from laminated sediments of the Nile deep-sea fan. Earth Planet. Sci. Lett. 364:98–110. doi: 10.1016/j.epsl.2013.01.009
  14. 14. Pachur HJ, Kröpelin S (1987) Wadi Howar: Paleoclimatic evidence from an extinct river system in the southeastern Sahara. Science 237:298–300. doi: 10.1126/science.237.4812.298
  15. 15. Williams MAJ, Talbot M, Aharon P, Salaam YA, Williams F, et al. (2006) Abrupt return of the summer monsoon 15,000 years ago: new supporting evidence from the lower White Nile Valley and Lake Albert. Quat. Sci. Rev. 25:2651–2665. doi: 10.1016/j.quascirev.2005.07.019
  16. 16. Ducassou E, Mulder T, Migeon S, Gonthier E, Murat A, et al. (2008) Nile floods recorded in deep Mediterranean sediments. Quat. Res. 70:382–391. doi: 10.1016/j.yqres.2008.02.011
  17. 17. Gutjahr M, Frank M, Stirling CH, Klemm V, van de Flierdt T, et al. (2007) Reliable extraction of a deepwater trace metal isotope signal from Fe–Mn oxyhydroxide coatings of marine sediments. Chem. Geol. 242 (3–4):351–370. doi: 10.1016/j.chemgeo.2007.03.021
  18. 18. Cohen RK, O'Nions AS, Siegenthaler R, Griffin WL (1988) Chronology of the pressure-temperature history recorded by a granulite terrain. Contrib. Miner. Petrol. 98:303–311. doi: 10.1007/bf00375181
  19. 19. Horwitz E, Chiarizia R, Dietz M (1992) A novel strontium-selective extraction chromatographic resin. Solvent Extract. Ion Exch. 10:313–336. doi: 10.1080/07366299208918107
  20. 20. Jacobsen SB, Wasserburg GJ (1980) Sm-Nd isotopic evolution of chondrites. Earth Planet. Sci. Lett. 50:139–155. doi: 10.1016/0012-821x(80)90125-9
  21. 21. Prahl FG, Mühlhausen LA, Zahnle DB (1988) Further evaluation of long-chain alkenones as indicators of paleoceanographic conditions. Geochim Cosmochim Acta 52:2303–2310. doi: 10.1016/0016-7037(88)90132-9
  22. 22. Conte MH, Sicre MA, Rühlemann C, Weber JC, Schulte S, et al. (2006) Global temperature calibration of the alkenone unsaturation index (Uk37′) in surface waters and comparison with surface sediments. Geochem. Geophys. Geosyst. 7:Q02005. doi: 10.1029/2005gc001054
  23. 23. Ternois Y, Sicre MA, Boireau A, Conte MH, Eglinton G (1997) Evaluation of long-chain alkenones as paleo-temperature indicators in the Mediterranean Sea. Deep Sea Res., Part I 44:271–286. doi: 10.1016/s0967-0637(97)89915-3
  24. 24. Castañeda I, Mulitza S, Schefuß E, Lopes dos Santos R, Sinninghe Damsté JS, et al. (2009) Wet phases in the Sahara/Sahel region and human migration patterns in North Africa. Proc. Nat. Acad. Science USA 106:20159–20163. doi: 10.1073/pnas.0905771106
  25. 25. Bray EE, Evans ED (1961) Distribution of normal paraffins as a clue to recognition of source beds. Geochim Cosmochim Acta 22:2–15. doi: 10.1016/0016-7037(61)90069-2
  26. 26. Hopmans EC, Schouten S, Pancost RD, van der Meer MTJ, Sinninghe Damsté JS (2000) Analysis of intact tetraether lipids in archaeal cell material and sediments by high performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry. Rapid Commun. Mass Spectrom. 14:585–589. doi: 10.1002/(sici)1097-0231(20000415)14:7<585::aid-rcm913>3.3.co;2-e
  27. 27. Schouten S, Huguet C, Hopmans EC, Kienhuis MVM, Sinninghe Damsté JS (2007) Analytical methodology for TEX86 paleothermometry by high-performance liquid chromatography/atmospheric pressure chemical ionization-mass spectrometry. Anal. Chem. 79:2940–2944. doi: 10.1021/ac062339v
  28. 28. Hopmans E, Weijers JWH, Schefuß E, Herfort L, Sinninghe Damsté JS, et al. (2004) A novel proxy for terrestrial organic matter in sediments based on branched and isoprenoid tetraether lipids. Earth Planet. Sci. Lett. 224:107–116. doi: 10.1016/j.epsl.2004.05.012
  29. 29. Emeis KC, Schulz H, Struck U. Rossignol-Strick M, Erlenheuser H, et al. (2003) Eastern Mediterranean surface water temperatures and δ18O composition during deposition of sapropels in the late Quaternary. Paleoceanogr. 18:PA1005. doi: 10.1029/2000pa000617
  30. 30. Castañeda I, Schefuß E, Pätzold J, Sinninghe Damsté JS, Weldeab S, et al. (2010) Millennial-scale sea surface temperature changes in the eastern Mediterranean (Nile River Delta region) over the last 27,000 years. Paleoceanogr. 25:PA1208. doi: 10.1029/2009pa001740
  31. 31. Bemis BE, Spero HJ, Bijma J, Lea DW (1998) Reevaluation of the oxygen isotopic composition of planktonic foraminifera: Experimental results and revised paleotemperature equations. Paleoceanogr. 13:150–160. doi: 10.1029/98pa00070
  32. 32. Waelbroeck C, Labeyrie L, Michel E, Duplessy JC, McManus JF, et al. (2002) Sea-level and deep water temperature changes derived from benthic foraminifera isotopic records. Quat. Sci. Rev. 21:295–305. doi: 10.1016/s0277-3791(01)00101-9
  33. 33. Gat JR, Shemesh A, Tziperman E, Hecht A, Georgopoulos D, et al. (1996) The stable isotope composition of waters of the eastern Medierranean Sea. J. Geophys. Res. 101:6441–6451. doi: 10.1029/95jc02829
  34. 34. Rohling EJ (2007) Progress in paleosalinity: Overview and presentation of a new approach. Paleoceanogr. 22:PA3215. doi: 10.1029/2007pa001437
  35. 35. Leduc G, Sachs JP, Kawka OE, Schneider RR (2012) Holocene changes in eastern equatorial Atlantic salinity as estimated by water isotopologues. Earth Planet. Sci. Lett. 362:151–162. doi: 10.1016/j.epsl.2012.12.003
  36. 36. Cockerton HE, Street-Perott FA, Leng MJ, Barker PA, Hortwood MSA, et al. (2013) Stable-isotope (H, O and Si) evidence for seasonal variations in hydrology and Si cycling from modern waters in the Nile Basin: Implications for interpreting the Quaternary record. Quat. Sci. Rev. 66:4–21. doi: 10.1016/j.quascirev.2012.12.005
  37. 37. Butzer KW (1980) Pleistocene History of the Nile Valley in Egypt and Lower Nubia. In Williams MAJ, Faure Heditors. The Sahara and the Nile. Quaternary Environments and Prehistoric Occupation in Northern Africa. Rotterdam: AA Balkema pp. 253–280.
  38. 38. Eglinton G, Hamilton RJ (1967) Leaf epicuticular waxes. Science 156:1322–1335. doi: 10.1126/science.156.3780.1322
  39. 39. Hoefs M, Rijpstra I, Sinninghe Damsté JS (2002) The influence of oxic degradation on the sedimentary biomarker record I: Evidence from Madeira Abyssal Plain turbidites. Geochim. Cosmochim. Acta 66:2719–2735. doi: 10.1016/s0016-7037(02)00864-5
  40. 40. Castañeda I, Schouten S (2011) A review of molecular organic proxies for examining modern and ancient lacustrine environments. Quat. Sci. Rev. 30:2851–2891. doi: 10.1016/j.quascirev.2011.07.009
  41. 41. Diefendorf AF, Freeman KH, Wing SL, Graham HV (2011) Production of n-alkyl lipids in living plants and implications for the geological past. Geochim. Cosmochim. Acta 75:7472–7485. doi: 10.1016/j.gca.2011.09.028
  42. 42. Jolly D, Harrison SP, Damnati B, Bonnefille R (1998) Simulated climate and biomes of Africa during the Late Quaternary: comparison with pollen and lake status data. Quat. Sci. Rev. 17:629–657. doi: 10.1016/s0277-3791(98)00015-8
  43. 43. Collins JA, Schefuß E, Heslop D, Mulitza S, Prange M, et al. (2011) Interhemispheric symmetry of the tropical African rainbelt over the past 23,000 years. Nature Geosci. 4:42–45. doi: 10.1038/ngeo1039
  44. 44. Padoan M, Garzanti E, Harlavan Y, Villa IM (2011) Tracing Nile sediment sources by Sr and Nd isotope signatures (Uganda, Ethiopia, Sudan). Geochim. Cosmochim. Acta 75:3627–3644. doi: 10.1016/j.gca.2011.03.042
  45. 45. Jung SJA, Davies GR, Ganssen G, Kroon D (2004) Stepwise Holocene aridification in NE Africa deduced from dust-borne radiogenic isotope records. Earth Planet. Sci. Lett. 221:27–37. doi: 10.1016/s0012-821x(04)00095-0
  46. 46. Marshall MH, Lamb HF, Huws D, Davies SJ, et al. (2011) Late Pleistocene and Holocene drought events at Lake Tana, the source of the Blue Nile. Global Planet. Change 78:147–161. doi: 10.1016/j.gloplacha.2011.06.004
  47. 47. Garcin Y, Melnick D, Strecker MR, Olago D, Tiercelin JJ (2012) East African mid-Holocene wet-dry transition in paleo-shorelines of Lake Turkana, northern Kenya Rift. Earth Planet. Sci. Lett. 331–332:322–334. doi: 10.1016/j.epsl.2012.03.016
  48. 48. Claussen M, Kubatzki C, Brovkin V, Ganopolski A, Hoelzmann P, et al. (1999) Simulation of an abrupt change in Saharan vegetation in the mid-Holocene. Geophys. Res. Lett. 26:2037–2040. doi: 10.1029/1999gl900494
  49. 49. Liu Z, Wang Y, Gallimore R, Notaro M, Prentice IC (2006) On the cause of abrupt vegetation collapse in North Africa during the Holocene: Climate variability vs. vegetation feedback. Geophys. Res. Lett. 33:L22709. doi: 10.1029/2006gl028062
  50. 50. Claussen M, Bathiany S, Brovkin V, Kleinen T (2013) Simulated climate-vegetation interaction in semi-arid regions affected by plant diversity. Nature Geosci. 6:954–958. doi: 10.1038/ngeo1962
  51. 51. Costa K, Russel J, Konecky B, Lamb H (2014) Isotopic reconstruction of the African Humid Period and Congo Air Boundary at Lake Tana, Ethiopia. Quat. Sci. Rev. 83:58–67. doi: 10.1016/j.quascirev.2013.10.031
  52. 52. Rohling EJ, Casford J, Abu-Zied R, Cooke S, Mercone D, et al.. (2002) Rapid Holocene climate change in the Eastern Mediterranean. In Hassan FAeditor. Droughts, Food and Culture: Ecological Change and Food Security in Africa's Later Prehistory. New York: Kluwer Academic/Plenum Publishers pp. 35–46.
  53. 53. Kim JH, Meggers H, Rimbu N, Lohmann G, Freudenthal T, et al. (2007) Impacts of the North Atlantic gyre circulation on Holocene climate off northwest Africa. Geology 35:387–390. doi: 10.1130/g23251a.1
  54. 54. Shanahan TM, Overpeck JT, Wheeler CW, Beck JW, Pigati JS, et al. (2006) Plaeoclimatic variations in West Africa from a record of late Pleistocene and Holocene lake level stands of Lake Bosumtwi, Ghana. Palaeogeogr. Palaeoclim., Palaeoeco. 242:287–302. doi: 10.1016/j.palaeo.2006.06.007
  55. 55. di Lernia S, Tafuri MA, Gallinaro M, Alhaique F, Balasse M, et al. (2013) Inside the “African Cattle Complex”: Animal burials in the Holocene Central Sahara. PLOSOne 8(2):e56879. doi: 10.1371/journal.pone.0056879
  56. 56. Dunne J, Evershed RP, Salque M, Cramp L, Bruni S, et al. (2012) First Dayring in green Sahara in the fifth millennium BC. Nature 486:390–394. doi: 10.1038/nature11186
  57. 57. Butzer KW (1976) Early Hydraulic Civilization in Egypt. Chicago: The University Chicago Press. 134 p.
  58. 58. Mayaux P, Bartholomé E, Massart M, Van Cutsem C, Cabral A, et al.. (2003) EUR 20665 EN - A Land Cover Map of Africa. Luxembourg: Office for Official Publication of the European Communities. 20p.
  59. 59. Laskar J, Robutel P, Joutel F, Gastineau M, Correia ACM, et al. (2004) A long term numerical solution for the insolation quantities of the Earth. Astronom. Astrophys. 428:261–285. doi: 10.1051/0004-6361:20041335