Potato virus Y (PVY, genus Potyvirus) causes substantial economic losses in solanaceous plants. Routine screening for PVY is an essential part of seed potato certification, and serological assays are often used. The commercial, commonly used monoclonal antibodies, MAb1128, MAb1129, and MAb1130, recognize the viral coat protein (CP) of PVY and distinguish PVYN strains from PVYO and PVYC strains, or detect all PVY strains, respectively. However, the minimal epitopes recognized by these antibodies have not been identified.
SPOT peptide array was used to map the epitopes in CP recognized by MAb1128, MAb1129, and MAb1130. Then alanine replacement as well as N- and C-terminal deletion analysis of the identified peptide epitopes was done to determine critical amino acids for antibody recognition and the respective minimal epitopes. The epitopes of all antibodies were located within the 30 N-terminal-most residues. The minimal epitope of MAb1128 was 25NLNKEK30. Replacement of 25N or 27N with alanine weakened the recognition by MAb1128, and replacement of 26L, 29E, or 30K nearly precluded recognition. The minimal epitope for MAb1129 was 16RPEQGSIQSNP26 and the most critical residues for recognition were 22I and 23Q. The epitope of MAb1130 was defined by residues 5IDAGGS10. Mutation of residue 6D abrogated and mutation of 9G strongly reduced recognition of the peptide by MAb1130. Amino acid sequence alignment demonstrated that these epitopes are relatively conserved among PVY strains. Finally, recombinant CPs were produced to demonstrate that mutations in the variable positions of the epitope regions can affect detection with the MAbs.
The epitope data acquired can be compared with data on PVY CP-encoding sequences produced by laboratories worldwide and utilized to monitor how widely the new variants of PVY can be detected with current seed potato certification schemes or during the inspection of imported seed potatoes as conducted with these MAbs.
Citation: Tian Y-P, Hepojoki J, Ranki H, Lankinen H, Valkonen JPT (2014) Analysis of Potato virus Y Coat Protein Epitopes Recognized by Three Commercial Monoclonal Antibodies. PLoS ONE 9(12): e115766. https://doi.org/10.1371/journal.pone.0115766
Editor: Amit Dhingra, Washington State University, United States of America
Received: August 12, 2014; Accepted: November 28, 2014; Published: December 26, 2014
Copyright: © 2014 Tian et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: The authors confirm that all data underlying the findings are fully available without restriction. All relevant data are within the paper and its Supporting Information files.
Funding: Financial support from the Academy of Finland (grant 1253126) and the Ministry of Agriculture and Forestry (grant 2383/312) is gratefully acknowledged. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Potato virus Y (PVY) is the type member of the genus Potyvirus (family Potyviridae), the largest genus of plant-infecting RNA viruses . PVY has a positive-sense single-stranded RNA genome (∼9700 nt, depending on the strain) containing a single open reading frame that encodes a large polyprotein cleaved into ten mature proteins by three virally encoded proteinases. An additional open reading frame (pipo, 75 codons) is created by frame-shifting in the region encoding protein P3 . PVY genome is encapsidated to filamentous particles comprised of the viral CP (267 amino acid residues) that is the most C′-terminal region of the polyprotein and released by the viral proteinase NIa .
PVY mainly infects plants of family Solanaceae and causes great yield losses in major crops, including potato (Solanum tuberosum L.) , tobacco (Nicotiana tabacum L.)  and pepper (Capsicum spp.) . Aphids transmit PVY nonpersistently in the field, and a short visit and probing of the plant by an aphid is sufficient for transmission. The use of insecticides is insufficient to prevent PVY spread, whereas spraying foliage with mineral oil to interfere with probing, and use of straw mulch to reduce the contrast between plants and soil, can reduce PVY transmission .
The most common and important sources of PVY to potato crops are seed tubers infected with PVY  and the plants growing from them, as they offer a PVY source to aphids for further dissemination of the virus in the field. Therefore, planting virus-indexed healthy seed potatoes is an essential prerequisite to control potato yield losses caused by PVY. Special seed potato production and certification systems are needed to ensure the quality. Visual inspection of virus symptoms in the foliage of seed potato crops in the field is done to rogue the infected plants, but it is not a reliable or practical means to detect PVY in all potato cultivars, because PVY symptoms are not always characteristic enough, other symptoms may mask PVY symptoms, and some PVY strains cause no symptoms in certain cultivars (for symptoms caused by two PVY isolates in different cultivars grown from infected seed tubers, visit http://www.helsinki.fi/ppvir/research/pvy/index.html). Furthermore, current-season infections may cause no symptoms in foliage, although the progeny tubers will be infected. Therefore, seed potatoes need to be indexed for PVY using virus-specific, sensitive diagnostic methods.
The most efficient means to control PVY is a potato cultivar's native resistance to PVY -. Resistance genes recognizing and conferring high levels (extreme) resistance to all PVY strains exist but are relatively rare in potato cultivars . Other resistance genes recognize only certain groups of PVY strains. They trigger a hypersensitive resistance response (HR) in potato and prevent PVY from spreading to other parts of the plants from the initial infection site. The HR genes Nytbr and Nctbr are common in potato cultivars –. The strains of PVY recognized by these genes are designated to strain groups PVYO and PVYC, respectively , . However, PVY strains not recognized by Nytbr and Nctbr have become prevalent in all potato production areas and are now the cause of major crop losses. These strains designated to strain group PVYN  have been less of a concern for potato production in past, because they are often symptomless or cause only mild symptoms and limited yield reduction in potato . However, the currently predominant PVYN strains are recombinants . They carry genomic segments of PVYO strains and cause acute diseases in potato, including necrotic symptoms in tubers and leaves, and are called NTN strains within the PVYN strain group. Therefore, it is important to detect PVY using antibodies recognizing specific strain groups, notably the PVYN, so to eliminate the seed lots carrying PVY strains that can overcome resistance in the locally grown potato cultivars.
Serological detection of PVY relies on detection of CP (virus particles) with polyclonal (PAb) or monoclonal antibodies (MAb) and is commonly carried out using the enzyme-linked immunosorbent assay (ELISA) , . Additionally, polymerase chain reaction (PCR)-based methods that detect viral nucleic acids are often used , , but they tend to be more costly, require more advanced laboratory facilities than ELISA, and may still require antibodies for immunocapture, i.e., trapping and concentrating virions from plant sap –. Studies on Potato virus A (PVA, genus Potyvirus) indicate that, in potyvirus particles, CP folds such that the N-terminus is exposed at the surface of the particles . Therefore, immunization of animals with potyvirus virions results in antibodies whose epitopes are mainly in the N-terminal region of CP .
Polyclonal antisera produced in animals immunized with PVY virions do not distinguish the PVY strain groups, but MAbs are needed . Early studies on MAbs to PVY showed that immunization of mice with PVYN strain 605 virions resulted in hybrid cell cultures producing antibodies specific to PVYN strains that did not detect PVYO or PVYC strains. On the other hand, many MAbs detected all PVY strains indiscriminately , indicating that they detected epitopes highly conserved in PVY CP and could be used for general detection of PVY. Since then, MAbs have been prepared for PVY strain group specific detection or general detection of PVY strains in many research laboratories and companies –, but the epitopes of only relatively few MAbs have been studied. Keller et al.  prepared a phage library expressing 12-amino acid long random peptides and used three MAbs produced for detection of PVY  to select peptides from the library. Alignment of the peptide sequences with the CP sequence of PVYN suggested that each MAb detected a different epitope located within the N′-proximal part of the CP, which was confirmed by detection of the recombinant N-proximal part (55 residues) of the PVYN CP with the MAbs. The residues of the epitopes crucial for detection were predicted but not verified experimentally. Comparison of the peptide sequences with the CP sequences of PVYN, PVYN-NTN, PVYO and PVYC suggested that one MAb was specific to PVYN (including PVYN-NTN) and two MAbs detected all PVY strains, but these specificities were not tested. Nikolaeva et al.  mapped the binding sites of three commercially available PVYN-specific MAbs in the N′-proximal region of CP using overlapping synthetic peptides of different length.
A single amino acid substitution can abolish or create an epitope recognized by a MAb . Chikh Ali et al.  concluded that a glycine residue in CP at position 29 allows detection of the PVYN isolate PVY-12 with MAb2 that is supposed to detect PVYO and PVYC strains , and also allows detection of PVY-12 with MAb 1F5 that reacts mainly with PVYN strains . In contrast, PVYN-like isolates carrying a glutamic acid residue at position 29 are detected only with MAb 1F5 . Karasev et al.  concluded that glutamine at position 98 of PVY CP is required for detection with MAb 1F5. Recently, surface plasmon resonance analysis was used successfully to test which anti-PVY CP MAbs share the same epitope, but the epitopes were not localized in CP .
There is little information about the minimal epitopes of any PVY-specific MAbs. Such information would be useful for predicting in silico cross-reactivity of antibodies  and which virus isolates may escape detection. Minimal epitopes can be determined using alanine replacement (alanine scanning) and/or N- and C-terminal deletion analyses of synthetic peptides, e.g., as reported with Potato mop-top virus (genus Pomovirus)  and Potato virus A (genus Potyvirus) . Therefore, the aim of this study was to determine the minimal epitopes of three commercial MAbs that are commonly used to detect PVY in potato, especially during seed potato certification. According to the supplier of the MAbs (Neogen Phytodiagnostics, Ayr, Scotland), it is believed that MAb1128 detects PVYN specifically and MAb1129 detects both PVYO and PVYC that are closely related , whereas MAb1130 detects all three strain groups of PVY. Hence, the three MAbs can be used to determine the overall incidence of PVY in seed potato lots or the specific incidence of PVYN vs. PVYO and PVYC. However, because the epitopes corresponding to the three MAbs in PVY CP are unknown, it is difficult to compare the molecular PVY strain data based on CP-encoding sequences or the detection by other MAbs and to predict how exclusively PVY isolates might be detected based on the three MAbs. Therefore, we determined the minimal epitopes recognized by the three MAbs using overlapping synthetic peptides, alanine scanning mutagenesis, peptide deletion analyses, and CP point mutants.
Mapping Epitopes on CP Using SPOT Peptide Array
SPOT peptide synthesis  was used to generate 20 aa–long overlapping peptides at 3-aa intervals covering CP of the PVYO strain SASA207 (GenBank accession no. AJ584851), PVYN strain 605 (X97895), and PVYC strain Adgen (AJ890348), and an additional potyvirus species, Potato virus V (PVV, genus Potyvirus, isolate Suomi, AJ278894). Each CP was therefore represented by 84 overlapping peptides on a modified cellulose membrane and probed individually with MAb1128, MAb1129, or MAb1130.
MAb1128 recognized five peptides in the N-terminal region of PVYN CP, but it did not react with the peptides of other PVY strains (Fig. 1, top left). The sequences of the MAb1128-reactive peptides (Fig. 1, top right) share a common motif, 25NLNKEKEK32 (aa numbering based on complete PVY CP). MAb1129 detected five peptides in the N-terminal region of both PVYO and PVYC (Fig. 1, middle left), but not PVYN, and the peptides share a common motif, 16RPEQGSIQSNP26 (Fig. 1, middle right). MAb1130 reacted with two peptides of each of the three PVY strains (Fig. 1, bottom left), and these peptides correspond to the N-proximal portion of CPs. The common peptide sequence detected by MAb1130 in the three PVY strains was 4TIDAGGSSKKDARPEQG20, but there were two aa substitutions in PVYN (S11T and R16K; Fig. 1, bottom right). PVV peptides were not recognized by any of the MAbs (Fig. 1).
A. Each CP of Potato virus Y (PVY) strains O (PVYO-SASA207), N (PVYN-605) and C (PVYC-Adgen), and Potato virus V (PVV), was represented by 84 overlapping 20-residue long peptides synthesized on the membrane. Each row of the membrane contained 20 peptides. Asterisks indicate the position of the most N-terminal peptide of each virus. The membrane was probed with each of the following antibodies against CP: MAb1128, MAb1129, and MAb1130 (panels to the left). Binding of the MAbs to peptides was detected with horseradish peroxidase–labeled anti-mouse antibodies and visualized by capturing the enhanced chemiluminescence signals on X-ray film. The peptides detected with the respective MAb are aligned to the right (+, positive detection; –, negative). The sequence common to all peptides detected with each MAb is demarcated with dashed vertical lines. The positions of the amino acid residues in CP are indicated on top of the alignment. B. Complete amino acid sequences of PVYN-605, PVYO-SASA207, PVYC-Adgen, and PVV-Suomi aligned using Clustal X 2.0. Dots indicate identical residues, and dashes indicate gaps.
The membrane was also probed with polyclonal rabbit antibodies raised against PVY virions. The strongest reactivity was against peptides representing the N-terminal-most 50 aa of CP in all strains of PVY (Fig. 2). A few peptides corresponding to other regions of CP, especially the C-terminus, were also detected, but signals were weaker. The polyclonal antibodies showed some, relatively weak cross-reactivity with peptides designed based on CP of PVV (Fig. 2).
The membrane shown in Fig. 1 was probed with polyclonal rabbit anti-PVY CP antibodies and signals were detected using an alkaline phosphatase–conjugated pig anti-rabbit antibody. The membrane sections containing the peptides of different viruses are framed.
Alanine Scanning and Deletion Analysis Define the Minimal Epitopes
The epitope-related peptides identified in the SPOT analysis were further analyzed to yield a fine map for each epitope. During alanine scanning mutagenesis, one residue at a time was replaced with an alanine, whereas in the C- and N-terminal deletion analysis, aa residues were removed one by one (S1 Fig.). The peptides analyzed, as identified by peptide screening (Fig. 1), were N-P5 (13KDAKQEQGSIQPNLNKEKEK32) recognized by MAb1128, O-P3 (7AGGSSKKDARPEQGSIQSNP26) recognized by MAb1129, and NP-1 (1GNDTIDAGGSTKKDAKQEQG20) recognized by MAb1130 (Fig. 3). Results obtained in the analysis of the aforementioned peptides were verified by analyzing another peptide detected with the MAb, as illustrated in S1 Fig. The new SPOT peptide array containing the mutated and shortened peptides was probed with MAb1128, MAb1129, and MAb1130. The C- and N-terminal deletions demonstrated that the minimal epitope of MAb1128 was 25NLNKEK30 (Fig. 3). Alanine scanning further showed that replacement of 25N or 27N with alanine weakened the recognition by MAb1128, and replacement of 26L, 29E, or 30K nearly precluded recognition. For MAb1129, the minimal epitope was 16RPEQGSIQSNP26 (Fig. 3). The most critical aa residues for recognition by MAb1129 were 22I and 23Q. The epitope of MAb1130 was close to the N-terminus of CP and defined by residues 5IDAGGS10 (Fig. 3). Recognition of the peptide by MAb1130 was abrogated by mutation of residue 6D and strongly reduced by mutation of 9G (Fig. 3).
Alanine scanning and deletion mapping of selected peptides were used to define the minimal epitope detected by each MAb. In alanine scanning, each residue at a time was replaced with an alanine, whereas in deletion mapping one residue at a time was removed from the N- or C-terminus of the peptide. The peptides subjected to the analyses were N-P5 (MAb1128), O-P3 (MAb1129), and N-P1 (MAb1130) shown in Fig. 1. Probing of the peptides with the MAbs was done as in Fig. 1. The chemiluminescence signals were quantified. Each bar and line represents the relative signal intensity compared with the corresponding wild-type peptide.
Epitope mapping in the aforementioned analysis was done using only three PVY strains. Therefore, the 60 most N-terminal CP residues of 369 PVY strains whose sequences are available in the curated Description of Plant Viruses database (http://www.dpvweb.net/)  were aligned to assess the conservation of the epitopes we identified. The variants are listed in Table 1, and the actual aa sequences are aligned in S1 Table. The MAb1128 epitope, 25NLNKEK30, was conserved in most PVY isolates designated to PVYN strains and variants, such NTN strains, but it was not found in the isolates designated to PVYO or PVYC strain groups. The epitope of MAb1130 was also quite conserved in the large majority of PVY isolates. The greatest variability was observed in the epitope of MAb1129 (Table 1).
Influence of Mutations in CP Epitopes on MAb Detection
Alanine scanning of peptides indicated that certain aa substitutions would lead to a lack of recognition of the correspondingly mutated CP by one or several MAbs. To verify these results using intact CP, His6-tagged wild-type and three mutated CPs of PVYN-605 and one mutated CP of PVYO-UK were expressed in Escherichia coli. The bacterial lysates were tested by western blot analysis using each of the MAbs.
Alanine scanning predicted that the substitution D6A would abolish recognition of PVY CP by MAb1130 (Fig. 3), and this result was verified by PVYN-605 showing the lack of the Mab1130::CP interaction (Fig. 4). The substitution D6N has been reported in a PVY isolate described from tobacco (NCBI accession no. X68222 ), and this mutation introduced to CP of PVYN-605 also abolished detection with MAb1130 (Fig. 4). However, both aforementioned CP mutants were detected with MAb1128 (Fig. 5).
The N-terminal 35 residues of CP in PVYO-SASA207, PVYN-605 and PVYC-Adgen are aligned, and the residue D6, which was mutated, is indicated by an arrow. Red highlighting denotes the minimal epitope recognized by MAb1130, and the conserved DAG motif is underlined. Crude proteins extracted from E. coli expressing an individual recombinant PVYN-605 CP or mutant were analyzed by SDS-PAGE and stained with Coomassie Blue to detect the proteins. Positions of molecular size markers (in kilodaltons) are shown to the left. Virions of the wild-type viruses PVYN-605 and PVYO-UK and proteins of E. coli transformed with an empty expression vector were included as positive and negative controls, respectively. DAG, AAG, and NAG indicate the bacterial lysates containing the recombinant CPs of the wild-type PVYN-605 and CPs carrying substitution D6A or D6N, respectively (wild-type CP carrying the DAG motif is marked with an asterisk). The corresponding western blot using MAb1130 is shown below the gel.
Crude proteins extracted from E. coli expressing the recombinant PVYO-UK or PVYN-605 CP and their mutants were analyzed by western blotting using a polyclonal antiserum (PAb) and the three MAbs against PVY CP. Positions of molecular size markers (in kilodaltons) are shown to the right. Virions of the wild-type viruses PVYO-UK and PVYN-NTN Nevski, and proteins of E. coli transformed with an empty expression vector [E. coli (-)] were included as positive and negative controls, respectively. The amino acid substitutions introduced to CPs and their positions are specified in the names of the mutant proteins shown at the top of the blot. Detection of virions with PAb (and PVYO-UK virions with MAb1129 and MAb1130) revealed dimers (ca. 70 kDa) of the CP besides the expected monomers (ca. 35 kDa), indicating the CP-CP interactions essential for virion formation were not fully denatured by the SDS treatment.
Hidaka et al.  reported a PVY isolate with three aa substitutions (R16K, P17L, G20D); among these, R16A reduced detection of the peptide with MAb1129 (Fig. 3). Introduction of these three aa substitutions to CP of PVYO-UK greatly reduced the signals of detection in western blots (Fig. 5).
PVYN-NTN Nevski was isolated from potato cv. Nevski in Finland and causes tuber necrosis symptoms in inoculated potato cultivars (Y. Tian and J. Valkonen, unpublished data). This PVY strain contains an aa substitution N25T in CP (NCBI accession no. JX432967) in the first residue of the epitope of MAb1128. PVYN-NTN Nevski was detected with MAb1128 (Fig. 5, Table 1), however, signals were weaker than for PVYN-605.
Alanine scanning with peptide N-P5 suggested that substitution N27A may weaken the interaction between MAb1128 and PVYN CP (Fig. 3). A PVYN strain with the N27I mutation has been reported (U09508; ), and this substitution was introduced to CP of PVYN-605. The mutant CP was detected with MAb1130 but not with MAb1128 (Fig. 5).
The bacterial lysates used in these experiments were also tested with MAbs using triple-antibody sandwich (TAS)-ELISA, and results were consistent with western blot analyses (Table 2). All PVY CPs and mutants expressed in E. coli were detected with anti-PVY PAb by double-antibody sandwich (DAS)-ELISA (Table 2).
Detection of PVY is an integral part of the seed potato certification schemes . Here, we characterized the epitopes of three commercially available MAbs used in the detection of the three common strain groups of PVY. The SPOT technique was utilized for epitope mapping and to pinpoint the critical residues required for PVY recognition . The results were further verified using site-directed mutagenesis of full-length recombinant PVY CPs. All of the MAb epitopes we studied were mapped to the N-terminal 30 residues of CP. Similarly, previous studies localized the epitopes of six different MAbs to the first 31  or 50 residues  of PVY CP. This region is the most exposed and variable part of the potyvirus CP . As shown by multiple alignments beyond this region, the aa sequence becomes more conserved when it begins to form the helix, and owing to its inaccessibility becomes less immunogenic and less suitable for detection of specific PVY strains. Consequently, also the PAb bound preferentially to the N-terminal peptides of CP, confirming that the N-terminus is the most immunogenic portion of the PVY CP. Previous studies using three independent PAb produced by immunization of rabbits with virions of PVYN found that PVY CP contains five immunodominant regions: two at the N-terminus (aa 1-25 and 29–39), two in the middle region (aa 70–95 and 163–170) and one at the C-terminus (aa 240–267) . Also our studies revealed weak but detectable interaction of PAb with some of the C-terminal peptides. Furthermore, antibodies raised against peptides corresponding to the C-terminal region of PVY CP can detect the intact CP .
The minimal epitope for MAb1130, 5IDAGGS10, was common to all studied PVY strain groups. When sequences from 369 different PVY isolates were aligned, only a few differences in the epitope region were found, thus indicating that MAb1130 would probably recognize most isolates without being able to distinguish between them. Roughly 90% of the studied strains had no changes in the epitope sequence, and even more strains maintained the central portion of the epitope—the sequence DAG. This sequence was also present in the CP of the control PVV strain, and the corresponding peptide showed reactivity with the PAb against PVY. The conservation of this epitope among PVY strains reflects the important function of the DAG motif as it allows the transmission of PVY and related viruses from plant to plant by aphids . Epitopes of other MAbs against PVY CP ,  have been characterized and found to partially overlap with the 5IDAGGS10 sequence identified in our present study, revealing that this region is highly immunogenic in PVY, as reported also on other potyviruses (e.g., , , ).
The minimal epitope recognized by MAb1129 was 16RPEQGSIQSNP26 present in O- and C-strains of PVY. The peripheral portions of this sequence are highly variable, but the middle portion, namely 18EQGSIQ23, is conserved throughout the PVYO and PVYC strains we evaluated, with only very few exceptions (Table 1, S1 Table). In N-strains, this CP epitope region varies, and the peptides from this region did not bind MAb1129.
The minimal epitope recognized by MAb1128, 25NLNKEK30, was present only in the PVYN strain CP. The corresponding sequence of PVYO and PVYC strains contained substitutions L26P and E29G, thus explaining why MAb1128 did not recognize these strains. According to the aa sequence alignment (S1 Table), most PVY isolates belonging to N-strain and the NTN variants contain this sequence and should therefore be detected by MAb1128. The epitopes of two other commercially available and commonly used PVYN-specific MAbs (SASA-N, and Neogen-N = MAb1051) have been localized to CP residues 22–30  and hence overlap with the epitope of MAb1128. In the course of the study, we detected PVYN-Nevski with MAb1128 and MAb1051 (Neogen-N) in both DAS-ELISA and triple antibody sandwich (TAS) ELISA, whereas Neogen-N does not detect the strain PVYNTN-AST in TAS-ELISA according to a previous study . Both PVY strains contain the minimal epitope 25NLNKEK30 of MAb1128. Therefore, the question whether or not MAb1128 and Neogen-N share the same epitope requires further study. Similar to Mab1128, substitution N27I prevents detection of the PVYN CP with SASA-N and Neogen-N .
Designation of PVY strains to the strain groups N, O and C is commonly done based on detection with MAbs or by phylogenetic analysis of CP (nucleotide or deduced aa) sequences. However, strain group identification is actually based on the biological differences of the PVY strain groups in terms of their ability to overcome resistance genes in potato or cause severe symptoms in potato and tobacco. However, recent studies show that the determinants of the biological differences, which are used to recognize the strain groups of PVY isolates , reside in the helper component proteinase (HCpro) of PVY –. It is therefore remarkable how well the serogroups determined by anti-CP MAbs correlate with the biological differences of the strain groups determined by HCpro. Interactions between CP and HCpro are essential in vector transmission of potyviruses  and involve the DAG motif besides other regions of the CP , . Furthermore, HCpro-CP interactions are probably essential during the infection cycle in host plants , . Therefore, it is plausible that high selection pressure directs concerted evolution of HCpro and CP , which is reflected in the functional phenotypes of PVY isolates.
MAbs to PVY HCpro have been produced, including MAb 8E1 that reacts with the HCpro of O and C strains, and MAb 8B9 reacting with the HCpro of isolates from all three strain groups PVY including N strains . It is possible that detection of PVY isolates with MAbs recognizing specific functional motifs of the multifunctional HCpro – could provide a higher resolution and more fine-tuned grouping of PVY isolates according to their biological differences. Furthermore, as proposed by Canto et al.  two decades ago, detection of PVY using MAbs to both HCpro and CP would be helpful for recognition of the highly virulent PVYN Wilga (PVYN:O) strains that have recombined the whole HCpro or segments thereof from PVYO , , . They cause tuber necrosis and other severe symptoms in potato and are widely distributed .
Taken together, the detailed MAb epitope analysis provided in this study should allow a sequence-based identification of the PVY serotypes and to predict whether particular isolates could escape detection or whether they will be detected by specific anti-CP MAbs. Our results help explain why different PVY strain groups can be distinguished using these MAbs. Furthermore, the data may be used for the generation of novel tools for detection of different PVY strains.
Materials and Methods
PVY detection reagent set, which contains rabbit PAb (1130-2, Neogen Phytodiagnostics) for coating, MAbs (TAS-ELISA) or alkaline phosphatase conjugated MAbs (DAS-ELISA) for probing, and horseradish peroxidase–conjugated polyclonal rabbit anti-mouse immunoglobulins (TAS-ELISA) was used (Neogen). Also polyclonal porcine anti-rabbit immunoglobulins were used (Dako, Carpinteria, CA, USA). Mab1128, Mab1129, Mab1130 and the rabbit polyclonal antiserum against PVY were produced using whole viruses as immunogens (Neogen).
Spot peptides  were synthesized with a fully automated MultiPep Spot robot (Intavis AG, Köln, Germany) on amino-polyethylene glycol–functionalized cellulose membranes (SynthoPlan APEG CE Membrane, AIMS Scientific Products, Product number AC-S01-12) via Fmoc chemistry. The PVY full-length CPs of different PVY strains were synthesized as 20-aa peptides (3-aa overlap), resulting in 84 peptides per PVY CP. The PVY strains were spotted on each membrane in the following order: PVYO (isolate SASA207; GenBank accession number AJ584851; ), PVYN (isolate 605; X97895; ), PVYC (isolate Adgen; AJ890348; ), and PVV (isolate Suomi; AJ278894; ). The initially identified MAb epitopes were further characterized by subjecting the relevant peptides to alanine scanning (residues replaced one by one with alanine) and to N- or C-terminal deletion (residues removed one by one).
Probing of SPOT Membranes
The assay was done as described . Briefly, antibodies and conjugates were diluted with Tris-buffered saline containing 0.05% (v/v) Tween 20 and supplemented with 3.0% skim milk (blocking buffer). The MAbs were used at 1 µg/ml and polyclonal antisera at 1∶1000 dilutions in blocking buffer. Secondary antibody incubations were done at 1∶1000 dilutions in blocking buffer. Antibody binding was visualized using enhanced chemiluminescence and X-ray film.
Mutagenesis and Expression of PVY CP in E. coli
Full-length PVY CP-encoding sequences were PCR-amplified with primers corresponding to the 5′-end and complementary to the 3′-end of PVYN605 and PVYOUK CP-encoding sequences and introducing BamHI and PstI restriction sites, respectively (Table 3). The amplified products were cloned into the expression vector pQE30 (Qiagen Hilden, Germany).
Four mutated CPs were also constructed (Table 3) using the Phusion Site-Directed Mutagenesis kit (Finnzymes, Espoo, Finland). The aspartic acid at position 6 (IDAGGS epitope) of PVYN-605 CP was mutated to alanine and asparagine to correspond to the sequences of PVYN-6A and PVYN-6N, respectively. Asparagine at position 27 (NLNKIKE epitope) of PVYN-605 CP was mutated to isoleucine. Arginine, proline, and glycine at positions 16, 17, and 20 of PVYO-UK CP were mutated to lysine, leucine, and aspartic acid, respectively (PVYO 16K17L20D). The CP of the PVY-Nevski isolate (25T instead of 25N) was also included in the study. All constructs generated were transformed into E. coli strain M15 (pREP4+) (Qiagen) for protein production.
Protein expression and purification was done as earlier . Briefly, cells were harvested by centrifugation at 11,000×g for 10 min, freezed in liquid nitrogen, and lysed by grinding on ice. The resultant lysate was suspended in ELISA extraction buffer (phosphate-buffered saline including 0.05% (v/v) Tween 20 and 2% (w/v) polyvinylpyrrolidone, pH 7.4) and fractioned via centrifugation at 15,000×g for 30 min at 4°C. The supernatant was analyzed with DAS- and TAS-ELISA and with SDS-PAGE. The His-tagged proteins used in western blotting were purified using the Ni-NTA strategy (Qiagen, Hilden, Germany) and were analyzed by SDS-PAGE and western blotting.
Alanine scanning and deletion mapping to define the minimal epitope detected by MAb1128 in PVYO–UK CP.
We thank Minna Rännäli for technical assistance, Jussi Karjula (Pohjoisen Kantanperuna Oy) for collaboration, and John Semple (Adgen Phytodiagnostics, Neogen Europe, Ltd.) for providing antibodies.
Conceived and designed the experiments: HR JH YT HL JPTV. Performed the experiments: HR JH YT. Analyzed the data: HR JH YT HL JPTV. Contributed reagents/materials/analysis tools: HR JH YT HL JPTV. Wrote the paper: JH YT JPTV.
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