The use of phylogenetic information in community ecology and conservation has grown in recent years. Two key issues for community phylogenetics studies, however, are (i) low terminal phylogenetic resolution and (ii) arbitrarily defined species pools.
We used three DNA barcodes (plastid DNA regions rbcL, matK, and trnH-psbA) to infer a phylogeny for 527 native and naturalized trees of Puerto Rico, representing the vast majority of the entire tree flora of the island (89%). We used a maximum likelihood (ML) approach with and without a constraint tree that enforced monophyly of recognized plant orders. Based on 50% consensus trees, the ML analyses improved phylogenetic resolution relative to a comparable phylogeny generated with Phylomatic (proportion of internal nodes resolved: constrained ML = 74%, unconstrained ML = 68%, Phylomatic = 52%). We quantified the phylogenetic composition of 15 protected forests in Puerto Rico using the constrained ML and Phylomatic phylogenies. We found some evidence that tree communities in areas of high water stress were relatively phylogenetically clustered. Reducing the scale at which the species pool was defined (from island to soil types) changed some of our results depending on which phylogeny (ML vs. Phylomatic) was used. Overall, the increased terminal resolution provided by the ML phylogeny revealed additional patterns that were not observed with a less-resolved phylogeny.
With the DNA barcode phylogeny presented here (based on an island-wide species pool), we show that a more fully resolved phylogeny increases power to detect nonrandom patterns of community composition in several Puerto Rican tree communities. Especially if combined with additional information on species functional traits and geographic distributions, this phylogeny will (i) facilitate stronger inferences about the role of historical processes in governing the assembly and composition of Puerto Rican forests, (ii) provide insight into Caribbean biogeography, and (iii) aid in incorporating evolutionary history into conservation planning.
Citation: Muscarella R, Uriarte M, Erickson DL, Swenson NG, Zimmerman JK, Kress WJ (2014) A Well-Resolved Phylogeny of the Trees of Puerto Rico Based on DNA Barcode Sequence Data. PLoS ONE 9(11): e112843. https://doi.org/10.1371/journal.pone.0112843
Editor: Damon P. Little, The New York Botanical Garden, United States of America
Received: January 15, 2014; Accepted: October 17, 2014; Published: November 11, 2014
This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Funding: This work was supported by NSF DEB 1050957 to MU, NSF DEB 1311367 to MU and RM, and the Smithsonian Institution. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
The use of phylogenetic information in community ecology and conservation has grown dramatically in recent years , , . This body of research has been largely stimulated by the idea that evolutionary relationships can provide insights into the historical processes governing assembly of local communities , , . From a conservation perspective, phylogenies may reveal aspects of biodiversity that are not observable from traditional metrics of species diversity , , , . By providing a historical context, phylogenies help merge our understanding of ecological, evolutionary, and biogeographic drivers of community composition .
One key issue for research in community phylogenetics is how to best estimate phylogenetic relationships among species in diverse communities (e.g., tropical forests). To date, the program Phylomatic  has become a primary method by which ecologists integrate phylogenetic information with analyses of community patterns (e.g., , , ). For plants, Phylomatic generates community phylogenies by pruning a megatree of angiosperms given a user-defined species list. This approach offers a repeatable and accessible way to obtain phylogenies using existing data (also see ), however, Phylomatic phylogenies typically have low or no taxonomic resolution among closely related species (e.g., within plant families or genera). Low taxonomic resolution can reduce statistical power for detecting nonrandom patterns of community structure ,  and can bias estimates of phylogenetic signal . Furthermore, because single genera often contain numerous species with diverse life-history characteristics (e.g., , ), resolving evolutionary relationships among congeners is critical for interpreting the link between patterns of phylogenetic community composition and the history of trait evolution. Finally, low taxonomic resolution can preclude inferences about biogeographic influences on local assemblages. The issue is particularly acute with respect to relatively recent evolutionary history (i.e., speciation events), which arguably represent a key connection between local and regional processes (see  and references therein).
In contrast to megatree approaches such as Phylomatic, phylogenies based on genetic data typically provide comparatively high taxonomic resolution. Generating molecular phylogenies, however, requires a significant investment of resources and expert knowledge. Additionally, determining how to estimate phylogenies among the very distantly related species that are typical of community-based phylogenies (as opposed to clade-based phylogenies) remains an active area of research. One potentially promising approach is to integrate existing information on evolutionary relationships in the form of a constraint tree . More research is required, however, to determine the influence of constraint trees on phylogenetic reconstruction and downstream analyses of community phylogenetic patterns.
Another characteristic of many existing studies of community phylogenetic structure lies in the lack of consistent methodology in defining species pools when testing hypotheses about mechanisms driving community assembly (e.g., competition versus environmental filtering) . Generally, these analyses are based on null models that compare an observed metric of phylogenetic composition (e.g., NRI, the net relatedness index) with a random expectation based on assemblages drawn from a regional species pool . In practice, studies often delimit the ‘regional pool’ as the set of species encountered in the study, regardless of the ecological significance of the study area boundaries (e.g., forest dynamics plots). Examining species assemblages within such arbitrarily defined regions can provide information on processes occurring at certain scales (e.g., , ). However, varying the spatial scale at which species pools are defined can provide important opportunities to evaluate the relative strength of local assembly processes (e.g., interactions that occur among neighboring trees) versus processes that occur over larger spatial and temporal scales (e.g., evolution and biogeography) and across broader environmental gradients (e.g., , , , , , ). For example, numerous studies in phylogenetic community ecology have shown that as the spatial (and taxonomic) extent of the species pool increases, the phylogenetic composition of local communities tends to appear increasingly ‘clustered’ (i.e., co-occurring species are more closely related than expected by random chance). Other studies have shown more mixed results (see references in , ), which may emerge, for example, if a larger species pool includes sister taxa absent from the smaller pool. In any case, scale-dependency of community patterns likely reflects the scales at which different assembly processes influence community structure , , , . As such, we can gain valuable insights on community assembly by adjusting species pools to suit particular hypotheses about the scales at which different assembly processes act , , , , , , , .
In this study, we used DNA sequence data to generate an island-wide phylogeny for nearly all of the native and naturalized tree species of Puerto Rico. Specifically, we used sequence data from three regions of plastid DNA which are commonly used as plant DNA barcodes (rbcL, matK, trnH-psbA; ) to resolve evolutionary relationships among 527 recognized species with a maximum likelihood (ML) approach. We compare phylogenetic resolution of two ML phylogenies (built with and without the use of an ordinal-level constraint tree) and a comparable phylogeny derived from Phylomatic. We then explore the implications of these different methods in a case study where we examined the phylogenetic structure of tree communities in 15 protected forests in Puerto Rico. These 15 forests span a wide variation in environmental conditions, providing an ideal template for evaluating the effects of local environmental variation on phylogenetic community structure within the island of Puerto Rico (Table 1). We addressed the following specific questions:
- How does the use of a constraint tree influence (i) the level of bootstrap support in a DNA barcode phylogeny of Puerto Rican trees, and (ii) the degree to which a molecular phylogeny corresponds with currently recognized taxonomic groups? We predicted that the constraint tree would provide higher levels of bootstrap support among unconstrained nodes and increase concordance with current taxonomy relative to the unconstrained analysis.
- How do patterns of community phylogenetic structure in Puerto Rican forests differ when based on a DNA barcode phylogeny versus a Phylomatic phylogeny? We predicted that an increase in statistical power provided by the higher resolution of a molecular phylogeny would lead to a stronger signal of non-random phylogenetic structure.
- How does phylogenetic structure in Puerto Rican forests change with respect to different species pool definitions? We predicted co-occurring species would tend to appear relatively phylogenetically clustered with respect to the full island species pool because of a strong role for environmental filtering across broad environmental gradients. We predicted that a more restricted species pool definition would reduce the level of phylogenetic clustering if niche differentiation (competitive exclusion) becomes more apparent at small spatial scales.
Materials and Methods
All necessary permits were obtained for the described study, which complied with all relevant regulations. Specifically, the Departmento de Recursos Naturales y Ambientales (DRNA) of Puerto Rico granted permit #2011-IC-046 to collect plant specimens in the state forests of Puerto Rico. Herbaria staff at the University of Puerto Rico, Rio Piedras and the US National Herbarium provided permission to sample tissue from their collections.
Study area and species
The island of Puerto Rico encompasses six Holdridge life zones  ranging from subtropical dry forest to subtropical rainforest in an area of 8,740 km2 . Mean annual precipitation ranges drastically, from ca. 700–4,500 mm yr−1 . The island’s complex geologic history is reflected in its rugged topography (0–1,338 m a.s.l.) and diverse parent soil materials, which include volcanic, limestone, alluvial, and ultramafic materials . Substantial existing data on the flora (e.g., , , ) provide a strong foundation for our work.
We created an initial list of Puerto Rican trees with the species list from the USFS Forest Inventory and Analysis (FIA) Caribbean field guide . With guidance from local experts (P. Acevedo-Rodríguez, F. Areces, F. Axelrod, M. Caraballo, J. Sustache, and P. Vives, personal communication), we modified this list by (1) updating nomenclature to be consistent with Acevedo-Rodríguez and Strong , (2) removing species occurring only under cultivation and (3) adding native and naturalized tree species known to occur in Puerto Rico but absent from the FIA list. Our final list of target species contained 594 species of seed plants representing 33 orders, 86 families, and 304 genera (Table S1). Of these, we were able to compile DNA sequence data for 523 (89%) species representing all 32 orders, 85 families (99%), and 287 genera (94%). The single excluded family (Cunoniaceae) is represented in Puerto Rico by a single rare species of shrub and most of the other species missing from our dataset are relatively uncommon and distributed widely throughout taxonomic groups. As a result, we do not expect the missing species to influence overall results of community phylogenetic analyses. However, it will be enlightening to include these species when sequence data become available in order to better understand the contributions of rare species to phylogenetic diversity .
Tissue collection and lab procedures
We acquired DNA sequence data from a variety of sources. Primarily, we obtained leaf tissue either from freshly collected specimens or existing herbarium sheets. For fresh specimens, we dried leaf tissue in silica gel prior to DNA extraction. Prior to depositing voucher specimens at the US National Herbarium (US), we verified species identifications by referring to the herbarium at the University of Puerto Rico, Río Piedras (UPRRP) and through consultation with local experts (F. Areces, F. Axelrod, P. Vives, personal communication). For 95 species, we collected leaf tissue from dry material sampled from herbarium specimens at UPRRP or US. DNA extraction, amplification and sequencing protocols followed Kress et al. . Specifically, we used the following lab procedures for fresh and dried leaf tissue. After disrupting tissue with a Tissuelyzer (Qiagen Cat. #85210), we incubated samples overnight at 55°C in a CTAB-based extraction buffer (AutoGen, Holliston, MA). Following incubation, we removed the supernatant and placed it in clean, 2 ml 96-well plate for submission to a DNA extraction robot (AutoGen 960, Holliston, MA). We hydrated DNA extractions in 100 mM Tris-HCl (pH 8.0) and then transferred them to Matrix barcode tubes (MatrixTechnologies Cat. # 3735) and stored them at −80°C. Working stocks of DNA were transferred to a microtiter plate and diluted 5× with water prior to PCR. We used routine PCR, with no more than three attempts per sample to recover PCR amplicons for each sample. The PCR cycling conditions were exactly the same for rbcL and trnH-psbA (95°C 3 min, [94°C 30 sec, 55°C 30 sec, 72°C 1 min]×35 cycles, 72°C 10 min) following procedures outlined in Kress and Erickson . The PCR cycling conditions for matK required lower annealing temperatures and more cycles (95°C 3 min [94°C 30 sec, 49°C 30 sec, 72°C 1 min]×40 cycles, 72°C 10 min) following Fazekas et al.  and included DMSO at a final concentration of 5%. We purified successful PCR reactions with a 56 diluted mixture of ExoSap (USB, Cat. # 78201). For sequencing, 2–4 ul of the purified PCR was used in a 12 ul reaction (0.8 ul BigDye terminator sequencing mixture (V3.1; ABI, Cat. 4337457), 2.0 ul of a 56 buffer (400 u Molar Tris-HCL pH 8.0), 1 ul of 1 uMolar primer and distilled water to volume). Sequencing of matK PCR products included DMSO to a final concentration of 4% in the reaction mixture. Cycling sequencing protocols were the same for all markers, (95°C 15 sec [94°C 15 sec, 50°C 15 sec, 60°C 4 min]×30 cycles). Following cycle sequencing, products were purified on a column of sephadex and sequence reactions were read on an ABI 3730 (Applied Biosystems).
We also incorporated existing sequence data for 143 species previously sequenced from the Luquillo Forest Dynamics Plot  and for 25 species from GenBank . We excluded 67 species from analyses for which we were unable to acquire reliable sequence data either because tissue was not available or because of failure during DNA sequencing (Table S1).
Sequence editing, alignment, and assembly
We used Geneious (R6, version 2.4.1; Biomatters Ltd.) to trim and assemble trace files for each marker into bidirectional contigs. Separately for each marker, we aligned sequences using SATé . SATé is an iterative algorithm that divides the original sequence data set using a tree-based decomposition; we aligned these smaller sets of sequences using MAFFT  and merged these sub-alignments into a global alignment without disrupting the individual sub-alignments using MUSCLE . SATé is particularly effective for conducting multiple sequence alignment among very distantly related taxa through the use of merging sub-alignments among related sequences, and has been widely applied for studies of very broad phylogenetic application , . We then concatenated the three separate marker alignments to produce an aligned three-gene matrix. Gaps were not coded and were treated as missing data in phylogenetic reconstruction.
We generated a phylogeny using maximum likelihood (ML) methods, implemented in RAxML (Stamatakis et al. 2005) via the CIPRES Science Gateway . Based on jModelTest2 , we modeled nucleotide substitution using a GTR+GAMMA model, with substitution rates estimated independently for each gene. We evaluated node support for the topology with the highest likelihood using 100 bootstrap runs. In addition, we trimmed Phylomatic reference tree R20120829  to use for comparative purposes. While other methods for phylogenetic reconstruction are available (e.g., parsimony), we focus here on a comparison between ML methods and a very commonly used method of generating phylogenies for community ecology (Phylomatic).
Rather than including densely sampled small taxonomic units, community phylogenies often contain smaller numbers of more distantly related species (e.g., 32 orders represented in our dataset, represented by 18 species, on average). Resolving both shallow and deep relationships requires distinct molecular data sets that are difficult to assemble. When strong prior information is available, one approach to confront this issue is to enforce some relationships through the use of a constraint tree (see for example ). In the case of our study, the Angiosperm Phylogeny Group III  represents the authoritative standard for current relationships up to the family level in angiosperms. However, within the AGP III phylogeny, relationships between species are generally not resolved beyond the family level, thus providing an ideal opportunity to use DNA barcodes to resolve these finer-scale relationships. To test the ability of a constraint tree to improve phylogenetic resolution among distantly related taxa, we repeated the ML analysis detailed above using the APG III phylogeny  to constrain the topology of ordinal and deeper nodes. This approach allowed the topology within each order to be resolved with DNA barcode sequence data while ordinal and deeper nodes were enforced a priori. We dated both the constrained and unconstrained ML phylogenies using PATHd8  with age constraints based on fossil records provided in the Appendix of Magallón & Castillo  (input files for our analyses are provided in Appendix S1 and S2). The constraints we used included one fixed age estimate for the angiosperm crown group and 35 minimal age estimates for other clades represented in our phylogeny (; Appendix S1 and S2). We used this approach because dated ultrametric trees are the standard for community phylogenetics studies; however, we also provide the undated, non-ultrametric trees in Appendix S3. To explore the distribution of uncertainty across the phylogeny, we calculated the proportion of recognized taxonomic groups (orders, families, and genera) that were found to be monophyletic in each analysis and the proportion of resolved nodes within each of these groups.
Case study: Phylogenetic composition of Puerto Rican forests
We measured the phylogenetic composition of 15 protected forests in Puerto Rico based on species occurrence data (presence/absence) from Little & Wadsworth  and Little et al. . As a synthesis of observations made by local experts, these volumes are the most commonly used references to describe tree composition of Puerto Rico’s protected forests. The 15 forests examined here span a wide range of environmental conditions (precipitation range: ca. 800–3,800 mm yr−1, elevation range: ca. 0–1,300 m a.s.l.) and occur across four main soil parent materials: unconsolidated, limestone, volcanic, and serpentine (Table 1, Fig. 1). We excluded taxa not included in our phylogeny – these accounted for only 2% of the total observations in the community dataset. With the remaining data, we quantified phylogenetic composition of each forest using the net relatedness index (NRI) and nearest taxon index (NTI) . These indices describe whether sets of co-occurring taxa are more or less closely related than random assemblages of equal species richness drawn from a pool of species. Specifically, NRI measures the average degree of relatedness among all members of the community and thus emphasizes deeper branches of the phylogenetic tree. In contrast, NTI is based on the average distance between closest relatives in each assemblage and thus emphasizes compositional patterns at the tips of the phylogeny . These metrics are calculated as: NRI = –(robs–mean(rrand))/sd(rrand), where r is either the co-occurring taxa (for NRI) or mean phylogenetic branch length separating nearest neighbors (for NTI). The observed value is robs and rrand is a distribution of values based on assemblages drawn from a species pool. We calculated NRI and NTI for each forest using two different species pools: the full list of species in our dataset (the ‘island pool’), and the list of species recorded from forests on the same soil parent material (the ‘soil pool’). For example, for Guánica forest (limestone soil), we calculated two values of NRI: one value (NRIISLAND) based on null assemblages drawn from the entire species list and another value (NRISOIL) based on the list of species recorded from all forests on limestone soil (the soil pool).
Forest life zones are coded as: subtropical dry (df-S), subtropical moist (mf-S), subtropical wet (wf-S), lower montane wet (wf-LM), subtropical rainforest (rf-S), lower montane rainforest (rf-LM). Refer to Table 1 for forest codes.
We computed NRI and NTI using the ses.mpd and ses.mntd functions of the ‘picante’ package  for R v 3.0.0 . We ran the analyses for 999 iterations and used the ‘taxa-labels’ null model. We chose this null model to control for the observed species occupancy rates and species richness of each forest. Positive values of NRI and NTI indicate phylogenetic clustering whereas negative values indicate phylogenetic evenness. We performed these analyses using the constrained ML 50% consensus tree and the Phylomatic phylogeny. We based these analyses on the constrained ML 50% consensus tree because it reflects the uncertainty of our phylogenetic hypothesis given our data, while also incorporating the strong evidence resolving deep relationships provided by the APG III constraint tree.
We quantified shifts in NRI and NTI values between the two species pool definitions using paired t-tests and we quantified the similarity of these values between phylogenies with Pearson’s correlation coefficient. In addition to overall patterns of community phylogenetic composition, we used the ‘nodesig’ algorithm in Phylocom v 4.2  to determine the particular clades that contribute significantly more or fewer species than expected to the composition of each forest.
DNA barcode sequences
From fresh tissue, we successfully recovered sequence data from 85%, 75%, and 94% of samples for rbcL, matK, trnH-psbA, respectively. The final three-gene alignment comprised 3,366 base pairs (549 bp for rbcL, 1,070 bp for matK, and 1,747 bp for trnH-psbA). The data matrix had 62.2% missing data (including gaps coded as missing data and species for which we did not recover sequence data). This amount is far more compact than previous alignments of the same three regions that used a nested partitioning of the trnH-psbA alignment, resulting in >95% missing data . Considering each region separately, the amount of missing data was 23.1%, 49.2%, and 82.1% for rbcL, matK, and trnH-psbA, respectively.
We provide the constrained and unconstrained ML trees, with bootstrap support, as well as the Phylomatic phylogeny used in our analyses in Appendix S3. Overall, we found relatively strong support for the majority of nodes in the both the constrained and unconstrained ML trees (Fig. 2). Across all nodes, 74% of nodes in the constrained ML tree received ≥50% bootstrap support and 52% received ≥80% bootstrap support. Considering only the 468 unconstrained nodes, 71% received ≥50% bootstrap support and 46% received ≥80% bootstrap support. The unconstrained ML tree had slightly lower levels of support with 68% of nodes receiving ≥50% support and 43% of nodes receiving ≥80% support. Both the constrained and unconstrained ML trees had higher resolution than the Phylomatic tree, in which only 52% of internal nodes were resolved. For the constrained ML tree, monophyly was supported for 91% of families and 87% of genera (monophyly of orders was constrained). In comparison, monophyly was supported for 72% of orders, 85% of families, and 87% of genera in the unconstrained ML tree. In both cases, the non-monophyly of currently recognized families related to the placement of taxa for which we did not have sequence data for all three barcode regions. For the constrained ML tree, the average proportion of nodes within orders, families, and genera with ≥50% bootstrap support was 0.81 (± SD 0.20), 0.87 (± SD 0.20), and 94% (± SD 0.19), respectively. For the unconstrained ML tree, the average proportion of nodes within orders, families, and genera with ≥50% bootstrap support was 0.92 (± SD 0.14), 0.89 (± SD 0.18), and 92% (± SD 0.20), respectively.
Case study: Phylogenetic composition of Puerto Rican forests
Some patterns of phylogenetic community structure varied with respect to the phylogeny and species pool used in analyses (Fig. 3). For NRI, which emphasizes tree-wide patterns, Guánica dry forest was significantly clustered (i.e., taxa were more closely related than expected) based on the full island species pool for both the ML and Phylomatic phylogenies (Fig. 3A). None of the other 14 forests departed from random expectations for NRI when based on the island pool. When considering the (reduced) soil species pools, the composition of the two wettest forests (Toro Negro and El Yunque, both located on volcanic soils) were significantly overdispersed (i.e., taxa were less closely related than expected), although the NRISOIL value for Toro Negro was only significant with respect to the ML phylogeny (Fig. 3B). For NTI, which emphasizes compositional patterns at the tips of the phylogeny, Cambalache forest was significantly clustered with respect to the full island species pool but only for the ML phylogeny (Fig. 3C). None of the forests had significantly nonrandom NTI values when the analyses were based on the (reduced) soil species pools, regardless of which phylogeny was used (Fig. 3D).
Leftmost panels are based on a null model using the full island species pool; right panels (B, D) are based on species pools restricted to primary soil types. Forests are sorted from left to right in order of their mean annual precipitation. Positive values indicate phylogenetic clustering and negative values indicate phylogenetic evenness. Filled symbols indicate values that are significantly different from a null model. Refer to Table 1 for forest codes.
None of the forests shifted from significantly clustered to significantly even when comparing NRI or NTI values based on the two different species pools. However, as we predicted, the (reduced) soil species pools caused both of these metrics to become more negative (i.e., decreased the signal of phylogenetic clustering) when calculated with the ML phylogeny (paired t-test: NRI: t = 2.79, df = 14, p<0.01; NTI: t = 4.34, df = 14, p<0.001). In contrast, these species pool definitions did not significantly change NRI or NTI when calculated with the Phylomatic phylogeny (paired t-test: NRI: t = 0.39, df = 14, p = 0.35; NTI: t = 0.28, df = 14, p = 0.39). Values of NRI calculated with each phylogeny were strongly correlated for both species pool definitions (island pool: Pearson’s r = 0.96, p<0.001; soil pool: Pearson’s r = 0.92, p<0.001) but values of NTI were less strongly correlated between these two phylogenies, and were not significantly correlated when based on the soil species pool (island pool: Pearson’s r = 0.60, p = 0.02; soil pool: Pearson’s r = 0.48, p = 0.06).
The node-based analysis identified particular clades that were relatively over- and under represented in each forest compared with a random expectation (Table 1) and, overall, the ML and Phylomatic phylogenies produced largely congruent results (Appendix S4). One of the more consistent results was that species belonging to Melastomataceae tended to be significantly underrepresented in relatively dry forests on limestone and serpentine soils (i.e., Guánica, Cambalache, Maricao, and Susúa) and relatively overrepresented in three relatively wet forests on volcanic soils (Guilarte, Luquillo and Toro Negro). Also, phylogenetic clustering of Guánica forest appears to be primarily driven by an overrepresentation of Fabaceae and Capparaceae, together with an underrepresentation of magnoliids, Ericales, and Melastomataceae (Appendix S4).
The island-wide phylogeny for Puerto Rican trees presented here represents the community phylogenetics approach applied at a regional scale with the use of DNA sequence data. Both the constrained and unconstrained ML phylogenies provided increased phylogenetic resolution in comparison with a corresponding Phylomatic tree, a predominant tree-building approach used in studies of community phylogenetics. In this study, the use of an ordinal-level constraint tree provided slightly higher phylogenetic resolution compared to the unconstrained analysis. In our case study, we uncovered patterns of nonrandom phylogenetic structure in Puerto Rican forests that depended on the phylogeny used as well as the scale at which the regional species pool was defined. Considering the rapidly increasing availability of DNA sequence data, future regional scale work in community phylogenetics will benefit from highly resolved phylogenies that include many taxa sampled across large areas and broad environmental gradients , , .
Comparison between phylogenies and taxonomic resolution
Although the ML phylogenies generated in this study were not completely resolved, the constrained 50% consensus tree did increase tip resolution by 22% in comparison with the Phylomatic tree. This relatively high degree of phylogenetic resolution has a number of important implications for community phylogenetic analyses , , . First, poorly resolved phylogenies tend to reduce statistical power for detecting nonrandom patterns of community structure (e.g., with NRI and NTI), an issue that appears to be more severe with larger phylogenies . Swenson  found that statistical power was most strongly reduced, however, when deeper nodes were unresolved (i.e. among orders and families) as opposed to more recent nodes (i.e. among species). As a result, we expect that the remaining unresolved nodes in our ML tree have a relatively small effect on analyses of phylogenetic structure for Puerto Rican tree communities because our constraint tree fixed the resolution of the deeper nodes. At the same time, the relatively deep nodes of the Phylomatic phylogeny are also resolved, suggesting that a reduction in statistical power for detecting nonrandom patterns between our ML tree and the Phylomatic tree may be most pronounced for metrics that focus on phylogenetic patterns among close relatives (e.g., NTI).
A second issue related to poorly resolved phylogenies is an upward bias when estimating phylogenetic signal . In other words, the tendency for close relatives to have similar functional traits tends to be overestimated when phylogenies are poorly resolved. This bias is of particular concern when examining patterns of phylogenetic community composition given the central role of phylogenetic signal of traits relevant for species co-occurrence . In general, the relatively high degree of tip resolution afforded by molecular data can strengthen inferences that rely on linking phylogenetic and functional patterns of community composition.
A major challenge in generating large-scale community phylogenies (and systematic biology, in general) is how to recover accurate phylogenetic relationships given limited data. Researchers have long debated the relative benefits of increasing sequence length versus increasing taxon sampling to improve the accuracy of phylogenetic reconstruction (e.g., , , , , ). This issue, however, has rarely been discussed in the context of community phylogenetics even though community-based analyses typically have relatively sparse taxon sampling compared to clade-based analyses. One implication of sparse taxon sampling is that long-branch attraction can reduce the accuracy of inferred topologies , . We confronted this potential issue by using a constraint tree to leverage strong prior information on deep phylogenetic relationships. In our case, the Angiosperm Phylogeny Group  provides a synthesis of well-supported relationships among the plant orders. Overall, bootstrap support for the constrained ML tree was higher than for the unconstrained tree although we had originally expected a stronger effect of using the constraint tree. The fairly high success of recovering recognized orders in the unconstrained analysis likely derives from the large sample size included in this study and the particular genes used; they were chosen, in part, for their high performance in phylogenetic analyses , .
While this study used a less sparse data matrix than previous work , the alignment procedure we use still resulted in a relatively sparse data matrix, particularly for the trnH-psbA region. The reason for this is that the SATé alignment algorithm knits together small alignments and introduces gaps when making a consensus alignment . Evidence suggests that introducing gaps does not affect the overall phylogenetic results as seen with the success of phylogenetic reconstructions using super matrix methods that produce extremely sparse alignments  and studies that successfully align non-coding ITS and chloroplast intergenic spacer data for very large phylogenetic assemblages . These studies suggest that missing data is not critical, particularly if one gene is shared among all taxa. Furthermore, while the effects of missing data on phylogenetic analyses are complex , several studies suggest that even taxa with large amounts of missing data can be accurately placed in phylogenies as long as the total number of characters sampled is large (e.g., , ). In addition, Wiens  showed that, in some cases, taxa with large amounts of missing data can improve overall phylogenetic accuracy, particularly with model-based phylogenetic methods (e.g., likelihood) ; but see . In our case, some instances of non-monophyly of recognized taxonomic groups were caused by individual taxa for which we did not have the full complement of three gene regions. Continued investigation of the influence of missing data on large phylogenetic analyses will help clarify the conditions under which missing data may decrease phylogenetic accuracy.
Case study: Phylogenetic composition of Puerto Rican forests
Our analysis of Puerto Rican tree communities provides an initial look at broad patterns of phylogenetic structure at a regional scale. For the most part, the ML and Phylomatic phylogenies provided congruent results in terms of NRI, which is a tree-wide metric of phylogenetic composition. In contrast, NTI values, which are more sensitive to variation at the tips of a phylogeny, were not surprisingly, more variable between the two phylogenies. Another difference between the two phylogenies was how the species pool influenced the results. Reducing the scale at which the regional species pool was defined (i.e., from the island to pools in each soil type) caused a decrease for both NRI and NTI when based on the ML phylogeny but no statistically significant change based on the Phylomatic phylogeny.
Based on the island species pool, one of the driest forests (Guánica, which is located at low elevation and on limestone soils) exhibited tree-wide phylogenetic clustering. Across all 15 forests, values of NRIISLAND tended to decline with mean annual precipitation, suggesting that drier forests generally comprise more phylogenetically clustered subsets of the island species pool than wetter forests. When evaluated with the reduced soil species pool, however, phylogenetic clustering of Guánica became random and only one forest in the moist life zone (Cambalache; located at low elevation and on limestone soils) had significantly clustered NTI with respect to the ML phylogeny only. The two wettest forests (Toro Negro and El Yunque, which are located on higher elevation volcanic soils) exhibited significant phylogenetic evenness in the NRI metric, although the value for Toro Negro was only significant with the ML phylogeny. One interpretation of these patterns is that water limitation represents a strong environmental filter in the dry forests and constrains the composition of local communities to the lineages that are able to persist under these harsh conditions. The issue of water stress in Puerto Rico may be exacerbated by the somewhat confounded nature of underlying geology and precipitation . Specifically, limestone soils tend to occur at lower elevations and receive less precipitation than volcanic soils. The combined influence of these variables likely compounds the effects of limited water availability for plants. In contrast, niche partitioning with respect to other factors (e.g., light use, vulnerability to pathogens) may play a stronger role in the wetter forests on volcanic soils, leading to a phylogenetically more diverse set of co-occurring species. One alternative explanation for this pattern is if in situ lineage diversification in Puerto Rico is a more important determinant of local species composition for higher elevation forests. For example, two closely related species of Tabebuia, T. rigida and T. schumanniana (Bignoniaceae) are endemic to El Yunque and Carite mountains, respectively .
We acknowledge three main limitations in our ability to interpret these patterns. First, we did not include information on species traits, which are relevant to their occurrence across environmental gradients. Our interpretations depend, in part, on the degree to which functional traits relevant to species occurrence along a gradient of water availability are phylogenetically conserved. Linking key functional traits with phylogenetic relatedness would help to more strongly identify the processes that underlie compositional variation among these forests , . Second, the occurrence data we used in this analysis lacks information on species abundances. Our analysis may not detect community assembly processes that are more strongly driven by species relative abundances (i.e., dominance) than the simple presence or absence. Finally, although our null model controls for species richness within each plot, statistical power for detecting nonrandom patterns is low for forests with low species richness . Nonetheless, the observed patterns provide a valuable starting point for future work aimed at addressing these limitations and providing additional insight on tree community variation across broad environmental gradients in Puerto Rico.
We found that values of NRI for each forest based on the different phylogenies were highly correlated whereas NTI values for each forest calculated with the two phylogenies were not correlated. These results reinforce the idea that low resolution among terminal tips (congeneric and confamilial taxa) may be especially problematic for recovering consistent patterns with NTI. In general, previous work has suggested that NRI may have greater power to detect nonrandom patterns of community phylogenetic structure than NTI , , .
In conclusion, our study provides a highly resolved community phylogeny for tropical trees at a regional scale: the island of Puerto Rico. We hope this regional perspective facilitates additional work to better understand the processes governing composition of local tree communities. Our case study confirms the value of a highly resolved phylogeny for detecting nonrandom patterns of phylogenetic community composition. Together with the extensive amount of existing data available in Puerto Rico on environmental conditions, land use history, species distributions and functional traits, we anticipate that the regional phylogeny provided here will help strengthen our historical perspective on the forces generating and structuring the diversity of Puerto Rican forests.
Table of molecular sequences from Puerto Rican tree species included in this analysis, including taxonomic information, collection details, GenBank accessions and voucher specimen details.
Input file for PATHd8  used to date the constrained ML tree.
Input file for PATHd8  used to date the unconstrained ML tree.
A Newick format file (.new) containing five phylogenies for Puerto Rican trees: (1) the dated ultrametric 50% consensus ML phylogeny using ordinal-level (APG-III) constraints, (2) the dated ultrametric 50% consensus ML phylogeny without topological constraints, (3) the undated non-ultrametric 50% consensus ML phylogeny using ordinal-level (APG-III) constraints, (4) the undated non-ultrametric 50% consensus ML phylogeny without topological constraints, (5) a corresponding Phylomatic phylogeny. Bootstrap support is provided for ML trees (i.e., trees 1–4; NA's in trees 1 and 3 refer to APG-III constrained nodes).
We extend our gratitude to the many people who helped collect and identify specimens including especially Fabiola Areces, Frank Axelrod, Alejandro Cubiña, Freddie Perez, José Sustache, Papo Vives, Alberto Areces, Pedro Acevedo, Marcos Caraballo, Víctor José Vega López, Silvia Bibbo, Christopher J. Nytch, Marcos Rodriguez, and T.J. Agin. Danilo Chinea and provided digitized occurrence data. All necessary permits were obtained for the described study, which complied with all relevant regulations. Specifically, we thank the Departmento de Recursos Naturales y Ambientales (DRNA) of Puerto Rico for permission to collect plant specimens in the forests of Puerto Rico under DRNA permit #2011-IC-046. We also thank the herbaria and staff at the University of Puerto Rico, Rio Piedras and the US National Herbarium for permission to sample tissue from their collections; the assistance of Pedro Acevedo was invaluable for this project. This manuscript benefitted from comments by Damon Little, Robert P. Anderson, Robin Chazdon, Joel Cracraft, Dustin Rubenstein, Camilo Sanin, Brian Weeks, and two anonymous reviewers.
Conceived and designed the experiments: RM MU DLE NGS JKZ WJK. Performed the experiments: RM DLE. Analyzed the data: RM DLE. Contributed reagents/materials/analysis tools: RM MU DLE WJK. Wrote the paper: RM MU DLE NGS JKZ WJK.
- 1. Losos JB (1996) Phylogenetic perspectives on community ecology. Ecology 77: 1344–1344.
- 2. Vellend M, Cornwell WK, Magnuson-Ford K, Mooers AØ (2011) Measuring phylogenetic biodiversity. In: Magurran AE, McGill BJ, editors. Biological diversity: frontiers in measurement and assessment. Oxford: Oxford University Press. 193–206.
- 3. Cavender-Bares J, Ackerly DD, H KK (2012) Integrating ecology and phylogenetics: the footprint of history in modern-day communities. Ecology 93: S1–S3.
- 4. Webb CO, Ackerly DD, McPeek MA, Donoghue MJ (2002) Phylogenies and community ecology. Annual Review of Ecology and Systematics 33: 475–505.
- 5. Cavender-Bares J, Kozak KH, Fine PVA, Kembel SW (2009) The merging of community ecology and phylogenetic biology. Ecology Letters 12: 693–715.
- 6. Vamosi SM, Heard SB, Vamosi JC, Webb CO (2009) Emerging patterns in the comparative analysis of phylogenetic community structure. Molecular Ecology 18: 572–592.
- 7. Vane-Wright RI, Humphries CJ, Williams PH (1991) What to protect? Systematics and the agony of choice. Biological Conservation 55: 235–254.
- 8. Williams PH, Humphries CJ, Vane-Wright RI (1991) Measuring biodiversity: taxonomic relatedness for conservation priorities. Australian Systematic Botany 4: 665–679.
- 9. Faith DP (1992) Conservation evaluation and phylogenetic diversity. Biological Conservation 61: 1–10.
- 10. Crozier RH (1997) Preserving the information content of species: genetic diversity, phylogeny, and conservation worth. Annual Review of Ecology and Systematics 28: 243–268.
- 11. Devictor V, Mouillot D, Meynard D, Jiguet F, Thuiller W, et al. (2010) Spatial mismatch and congruence between taxonomic, phylogenetic and functional diversity: the need for integrative conservation strategies in a changing world. Ecology Letters 13: 1030–1040.
- 12. Ricklefs RE (1987) Community diversity: relative roles of local and regional processes. Science 235: 167–171.
- 13. Webb CO, Donoghue MJ (2005) Phylomatic: tree assembly for applied phylogenetics. Molecular Ecology Notes 5: 181–183.
- 14. Kembel SW, Hubbell SP (2006) The phylogenetic structure of a neotropical forest tree community. Ecology 87: 86–99.
- 15. Willis CG, Ruhfel B, Primack RB, Miller-Rushing AJ, Davis CC (2008) Phylogenetic patterns of species loss in Thoreau’s woods are driven by climate change. Proceedings of the National Academy of Sciences 105: 17029–17033.
- 16. Kraft NJB, Ackerly DD (2010) Functional trait and phylogenetic tests of community assembly across spatial scales in an Amazonian forest. Ecological Monographs 80: 401–422.
- 17. Beaulieu JM, Ree RH, Cavender-Bares J, Weiblen GD, Donoghue MJ (2012) Synthesizing phylogenetic knowledge for ecological research. Ecology 93: S4–S13.
- 18. Swenson NG (2009) Phylogenetic resolution and quantifying the phylogenetic diversity and dispersion of communities. PLoS ONE 4: e4390–e4390.
- 19. Kress WJ, Erickson DL, Jones FA, Swenson NG, Perez R, et al. (2009) Plant DNA barcodes and a community phylogeny of a tropical forest dynamics plot in Panama. Proceedings of the National Academy of Sciences 106: 18621–18626.
- 20. Davies JT, Kraft NJB, Salamin N, Wolkovich EM (2012) Incompletely resolved phylogenetic trees inflate estimates of phylogenetic conservatism. Ecology 93: 242–247.
- 21. Cavender-Bares J, Ackerly DD, Baum DA, Bazzaz FA (2004) Phylogenetic overdispersion in Floridian Oak communities. The American Naturalist 163: 823–843.
- 22. Sedio BE, Wright SJ, Dick CW (2012) Trait evolution and the coexistence of a species swarm in the tropical forest understory. Journal of Ecology 100: 1183–1193.
- 23. Ricklefs R, Jenkins DG (2011) Biogeography and ecology: towards the integration of two disciplines. Philosophical Transactions of the Royal Society B-Biological Sciences 366: 2438–2448.
- 24. Kress WJ, Erickson DL, Swenson NG, Thompson J, Uriarte M, et al. (2010) Advances in the use of DNA barcodes to build a community phylogeny for tropical trees in a Puerto Rican forest dynamics plot. PLoS ONE 5: e15409.
- 25. Uriarte M, Swenson NG, Chazdon RL, Comita LS, Kress WJ, et al. (2010) Trait similarity, shared ancestry and the structure of neighbourhood interactions in a subtropical wet forest: implications for community assembly. Ecology Letters 13: 1503–1514.
- 26. Hardy OJ (2008) Testing the spatial phylogenetic structure of local communities: statistical performances of different null models and test statistics on a locally neutral community. Journal of Ecology 96: 914–926.
- 27. de Bello F, Price JN, Münkemüller T, Liira J, Zobel M, et al. (2012) Functional species pool framework to test for biotic effects on community assembly. Ecology 93: 2263–2273.
- 28. Lessard J-P, Belmaker J, Myers JA, Chase JM, Rahbek C (2012) Inferring local ecological processes amid species pool influences. Trends in Ecology & Evolution 27: 600–607.
- 29. Eiserhardt WL, Svenning JC, Borchsenius F, Kristiansen T, Balslev H (2013) Separating environmental and geographical determinants of phylogenetic community structure in Amazonian palms (Arecaceae). Botanical Journal of the Linnean Society 171: 244–259.
- 30. Brunbjerg AK, Cavender-Bares J, Eiserhardt WL, Ejrnæs R, Aarssen LW, et al. (2014) Multi-scale phylogenetic structure in coastal dune plant communities across the globe. Journal of Plant Ecology 7: 101–114.
- 31. Münkemüller T, Gallien L, Lavergne S, Renaud J, Roquet C, et al. (2014) Scale decisions can reverse conclusions on community assembly processes. Global Ecology and Biogeography 23: 620–632.
- 32. Parmentier I, Réjou-Méchain M, Chave J, Vleminckx J, Thomas DW, et al.. (2014) Prevalence of phylogenetic clustering at multiple scales in an African rain forest tree community. Journal of Ecology: n/a-n/a.
- 33. Pickett STA, Bazzaz FA (1978) Organization of an assemblage of early successional species on a soil moisture gradient. Ecology 59: 1248–1255.
- 34. Silvertown J, Dodd M, Gowing D, Lawson C, McConway K (2006) Phylogeny and the hierarchical organization of plant diversity. Ecology 87: 39–49.
- 35. Ackerly DD, Cornwell W (2007) A trait-based approach to community assembly: partitioning of species trait values into within- and among-community components. Ecology Letters 10: 135–145.
- 36. Cavender-Bares J, Keen A, Miles B (2006) Phylogenetic structure of Floridian plant communities depends on taxonomic and spatial scale. Ecology 87: S109–S122-S109-S122.
- 37. Swenson NG, Enquist BJ, Thompson J, Zimmerman JK (2007) The influence of spatial and size scale on phylogenetic relatedness in tropical forest communities. Ecology 88: 1770–1780.
- 38. Lessard J-P, Borregaard MK, Fordyce JA, Rahbek C, Weiser MD, et al. (2012) Strong influence of regional species pools on continent-wide structuring of local communities. Proceedings of Royal Society B 279: 266–274.
- 39. Swenson NG, Umaña MN (2014) Phylofloristics: an example from the Lesser Antilles. Journal of Plant Ecology 7: 166–175.
- 40. Holdridge LR (1947) Determination of world plant formations from simple climatic data. Science 105: 367–368.
- 41. Ewel JJ, Whitmore JL (1973) The ecological life zones of Puerto Rico and the U.S. Virgin Islands. USDA Forest Service, Institute of Tropical Forestry.
- 42. Daly C, Helmer EH, Quiñones M (2003) Mapping the climate of Puerto Rico, Vieques and Culebra. International Journal of Climatology 23: 1359–1381.
- 43. Bawiec WJ (1998) Geology, geochemistry, geophysics, mineral occurrences, and mineral resource assessment for the commonwealth of Puerto Rico: Open-File Report 98–38, Geological Survey (U.S.).
- 44. Axelrod FS (2011) A systematic vademecum to the vascular plants of Puerto Rico. Río Piedras, PR: University of Puerto Rico.
- 45. Acevedo-Rodríguez P, Strong MT (2011) Flora of the West Indies: catalogue of the seed plants of the West Indies. Washington, D.C.: Smithsonian Institution, National Museum of Natural History.
- 46. USFS (2006) Forest inventory and analysis national core field guide: Caribbean version 3.0. United States Forest Service.
- 47. Mi X, Swenson NG, Valencia R, Kress WJ, Erickson DL, et al. (2012) The contribution of rare species to community phylogenetic diversity across a global network of forest plots. American Naturalist 180: E17–30.
- 48. Kress WJ, Erickson DL (2007) A two-locus global DNA barcode for land plants: the coding rbcL gene complements the non-coding trnH-psbA spacer region. PLoS ONE 2: e508
- 49. Fazekas AJ, Burgess KS, Kesanakurti PR, Graham SW, Newmaster SG, et al. (2008) Multiple multilocus DNA barcodes from the plastid genome discriminate plant species equally well. PlosONE 3: e2802 (doi:https://doi.org/2810.1371/journal.pone.0002802).
- 50. Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Sayers EW (2010) GenBank. Nucleic Acids Research 38: D46–D51.
- 51. Liu K, Warnow TJ, Holder MT, Nelesen SM, Yu J, et al. (2012) SATé-II: very fast and accurate simultaneous estimation of multiple sequence alignments and phylogenetic trees. Systematic Biology 61: 90–106.
- 52. Katoh K, Kuma K, Toh H, Miyata T (2005) MAFFT version 5: improvement in accuracy of multiple sequence alignment. Nucleic Acids Research 33.
- 53. Edgar R (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32: 1792–1797.
- 54. Schoch CL, Crous PW, Groenewald JZ, Boehm EWA, Burgess TI, et al. (2009) A class-wide phylogenetic assessment of Dothideomycetes. Studies in Mycology 64: 1–15-S10.
- 55. Kivlin SN, Hawkes CV, Treseder KK (2011) Global diversity and distribution of arbuscular mycorrhizal fungi. Soil Biology and Biochemistry 43: 2294–2303.
- 56. Miller MA, Pfeiffer W, Schwartz T. Creating the CIPRES Science Gateway for inference of large phylogenetic trees; 2010 14 Nov. 2010; New Orleans, LA. 1–8.
- 57. Darriba D, Taboada GL, Doallo R, Posada D (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9: 772.
- 58. Stevens PF (2001 onwards) Angiosperm Phylogeny Website. Version 12, July 2012 [and more or less continuously updated since]. Available: www.mobot.org/MOBOT/research/APweb.
- 59. Smith SA, Beaulieu J, Donoghue MJ (2009) Mega-phylogeny approach for comparative biology: an alternative to supertree and supermatrix approaches. BMC Evolutionary Biology 9: 37.
- 60. APG III (2009) An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG III. Botanical Journal of the Linnean Society 161: 105–121.
- 61. Britton T, Anderson CL, Jacquet D, Lundqvist S, Bremer K (2007) Estimating divergence times in large phylogenetic trees. Systematic Biology 56: 741–752.
- 62. Magallón S, Castillo A (2009) Angiosperm diversification through time. American Journal of Botany 96: 349–365.
- 63. Little EL, Wadsworth FH (1964) Common trees of Puerto Rico and the Virgin Islands. Washington, DC: USDA Forest Service.
- 64. Little EL, Woodbury RO, Wadsworth FH (1974) Trees of Puerto Rico and the Virgin Islands. Washington, DC: USDA Forest Service.
- 65. Kembel SW, Cowan PD, Helmus MR, Cornwell WK, Morlon H, et al. (2010) Picante: R tools for integrating phylogenies and ecology. Bioinformatics 26: 1463–1464.
- 66. R Development Core Team (2012) R: A language and environment for statistical computing. v2.9.2 ed. Vienna, Austria: R Foundation for Statistical Computing.
- 67. Webb CO, Ackerly DD, Kembel SW (2008) Phylocom: software for the analysis of phylogenetic community structure and trait evolution. Bioinformatics 24: 2098–2100.
- 68. Swenson NG, Erickson DL, Mi XC, Bourg NA, Forero-Montaña J, et al.. (2012) Phylogenetic and functional alpha and beta diversity in temperate and tropical tree communities.
- 69. Swenson NG (2013) The assembly of tropical tree communities – the advances and shortcomings of phylogenetic and functional trait analyses. Ecography 36.
- 70. Mayfield MM, Levine JM (2010) Opposing effects of competitive exclusion on the phylogenetic structure of communities. Ecology Letters 13: 1085–1093.
- 71. Rannala B, Huelsenbeck JP, Yang Z, Nielsen R (1998) Taxon sampling and the accuracy of large phylogenies. Systematic Biology 47: 702–710.
- 72. Poe S, Swofford DL (1999) Taxon sampling revisited. Nature 398: 299–300.
- 73. Felsenstein J (2004) Inferring phylogenies. Sunderland, Massachusetts: Sinauer Associates.
- 74. Heath TA, Hedtke SM, Hillis DM (2008) Taxon sampling and the accuracy of phylogenetic analyses. Journal of Systematics and Evolution 46: 239–257.
- 75. Nabhan AR, Sarkar IN (2012) The impact of taxon sampling on phylogenetic inference: a review of two decades of controversy. Briefings in Bioinformatics 13: 122–134.
- 76. Stefanović S, Rice DW, Palmer JD (2004) Long branch attraction, taxon sampling, and the earliest angiosperms: Amborella or monocots? BMC Evolutionary Biology 4: 35.
- 77. CBOL Plant Working Group (2009) A DNA barcode for land plants. Proceedings of the National Academy of Sciences 106: 12794–12797.
- 78. McMahon MM, Sanderson MJ (2006) Phylogenetic supermatrix analysis of GenBank sequences from 2228 Papilionoid Legumes. Systematic Biology 55: 818–836.
- 79. Edwards E, Smith SA (2010) Phylogenetic analyses reveal the shady history of C4 grasses. Proceedings of the National Academy of Sciences 107: 2532–2538.
- 80. Wiens JJ (2006) Missing data and the design of phylogenetic analyses. Journal of Biomedical Informatics 39: 34–42.
- 81. Phillipe H, Snell EA, Bapteste E, Lopez P, Holland PWH, et al. (2004) Phylogenomics of eukaryotes: impact of missing data on large alignments. Molecular Biology and Evolution 21: 1740–1752.
- 82. Wiens JJ, Morrill MC (2011) Missing data in phylogenetic analysis: reconciling results from simulations and empirical data. Systematic Biology 60: 719–731.
- 83. Wiens JJ (1998) Does adding characters with missing data increase or decrease phylogenetic accuracy? Systematic Biology 47: 625–640.
- 84. Poe S (2003) Evaluation of the strategy of long-branch subdivision to improve the accuracy of phylogenetic methods. Systematic Biology 52: 423–428.
- 85. Miller GL, Lugo AE (2009) Guide to the ecological systems of Puerto Rico. Gen. Tech. Rep. IITF-GTR-35. In: United States Department of Agriculture, Forest Service, International Institute of Tropical Forestry, editors. San Juan, PR. 437.
- 86. Adler PB, Fajardo A, Kleinhesselink AR, Kraft NJB (2013) Trait-based tests of coexistence mechanisms. Ecology Letters 16: 1294–1306.
- 87. Gotelli NJ, Ulrich W (2012) Statistical challenges in null model analysis. Oikos 121: 171–180.
- 88. Letcher SG (2010) Phylogenetic structure of angiosperm communities during tropical forest succession. Proceedings of the Royal Society B: Biological Sciences 277: 97–104.
- 89. Letcher SG, Chazdon RL, Andrade ACS, Bongers F, van Breugel M, et al. (2012) Phylogenetic community structure during succession: evidence from three neotropical forest sites. Perspectives in Plant Ecology, Evolution and Systematics 14: 79–87.
- 90. Gould WA, Alarcon C, Fevold B, Jimenez ME, Martinuzzi S, et al.. (2008) The Puerto Rico Gap Analysis Project volume 1: land cover, vertebrate species distributions, and land stewardship. Gen. Tech. Rep. IITF-39.
- 91. Gesch DB (2007) The National Elevation Dataset. In: Maune D, editor. Digital Elevation Model Technologies and Applications: The DEM Users Manual. 2nd Edition ed. Bethesda, Maryland: American Society for Photogrammetry and Remote Sensing. 99–118.