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Correction: Essential Role of TEA Domain Transcription Factors in the Negative Regulation of the MYH 7 Gene by Thyroid Hormone and Its Receptors

  • The PLOS ONE Staff

Correction: Essential Role of TEA Domain Transcription Factors in the Negative Regulation of the MYH 7 Gene by Thyroid Hormone and Its Receptors

  • The PLOS ONE Staff
PLOS
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The image for Figure 4 is incorrect. Please see the correct Figure 4 here.

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Figure 4. T3-bound TRs (T3/TRs) repress TEAD-induced activity of the MYH7 promoter.

(A) and (B) T3/TRβ1 represses promoter activity of the MYH7 gene induced by TEAD-3 (A) or TEAD-1 (B). MYH7-CAT (wild-type) was transfected into CV-1 cells along with the expression plasmid for human TRβ1, mouse TEAD-3, or TEAD-1. *, P<0.05; ***, P<0.001 of T3 (−) vs. T3 (+). (C) Dose dependency of the amount of TRβ1 expressed. MYH7-CAT (2.0 µg) was co-transfected with the expression plasmid for human TRβ1 (0–0.8 µg) into CV-1 cells along with or without mouse TEAD-3 (0.2 µg) under the same conditions as those described in (A). In the left panel, CAT activities in the presence or absence of 1 µM T3 are indicated. The results are means ± S.D. for three independent experiments. *, P<0.05; ***, P<0.001 of T3 (−) vs. T3 (+). #, P<0.05. In the right panel, TRβ1 dose-dependency is indicated as fold repression. CAT activity without T3 was divided by that with 1 µM T3 to calculate fold activation. #, P<0.05. (D) Dose dependency of T3 or GC-1. MYH7-CAT, TEAD-3, and TRβ1 were expressed in CV-1 cells under the same conditions as those described in (A) and 0–1000 nM of T3 or GC-1 was supplemented. The results are means ± S.D. for three independent experiments. *, P<0.05 vs. TEAD-3 plus TRβ1 with vehicle. (E) TRα1 as well as TRβ1 inhibit the MYH7 promoter by T3. MYH7-CAT, TEAD-3, and FLAG-tagged TRα1 (gray bar) or TRβ1 (solid bar) were expressed in CV-1 cells under the same conditions as described in (A), and 0–1000 nM of T3 was supplemented. The expression of FLAG-tagged TRα1 and TRβ1 transfected into CV-1 cells were demonstrated by Western blot with antibody against FLAG and N-terminal region of TRβ1 [16], [26], [27], respectively (inset). Solid arrowhead, TRα1; solid arrow, TRβ1; open arrowheads, non-specific bands; cont, empty plasmid. The numbers on the left side of each panel indicate molecular mass markers (kDa). The results are means ± S.D. for three independent experiments. *, P<0.05 of T3 (−) vs. T3 (+). In these reporter assays (A–E), the expression level of pCMV–CAT was adjusted to a value of 100.

http://dx.doi.org/10.1371/journal.pone.0088610.g004

Reference

  1. 1. Iwaki H, Sasaki S, Matsushita A, Ohba K, Matsunaga H, et al. (2014) Essential Role of TEA Domain Transcription Factors in the Negative Regulation of the MYH 7 Gene by Thyroid Hormone and Its Receptors. PLoS ONE 9(4): e88610 doi:10.1371/journal.pone.0088610.