Patients with chronic known or unknown interstitial lung disease (ILD) may present with severe respiratory flares that require intensive management. Outcome data in these patients are scarce.
Patients and Methods
Clinical and radiological features were collected in 83 patients with ILD-associated acute respiratory failure (ARF). Determinants of hospital mortality and response to corticosteroid therapy were identified by logistic regression.
Hospital and 1-year mortality rates were 41% and 54% respectively. Pulmonary hypertension, computed tomography (CT) fibrosis and acute kidney injury were independently associated with mortality (odds ratio (OR) 4.55; 95% confidence interval (95%CI) (1.20–17.33); OR, 7.68; (1.78–33.22) and OR 10.60; (2.25–49.97) respectively). Response to steroids was higher in patients with shorter time from hospital admission to corticosteroid therapy. Patients with fibrosis on CT had lower response to steroids (OR, 0.03; (0.005–0.21)). In mechanically ventilated patients, overdistension induced by high PEEP settings was associated with CT fibrosis and hospital mortality.
Citation: Zafrani L, Lemiale V, Lapidus N, Lorillon G, Schlemmer B, Azoulay E (2014) Acute Respiratory Failure in Critically Ill Patients with Interstitial Lung Disease. PLoS ONE 9(8): e104897. https://doi.org/10.1371/journal.pone.0104897
Editor: Shervin Assassi, University of Texas Health Science Center at Houston, United States of America
Received: April 24, 2014; Accepted: July 16, 2014; Published: August 12, 2014
Copyright: © 2014 Zafrani et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: The authors confirm that all data underlying the findings are fully available without restriction. All relevant data are within the paper and its Supporting Information files.
Funding: The authors have no support or funding to report.
Competing interests: The authors have declared that no competing interests exist.
Interstitial lung disease (ILD) is a group of disorders that occur either in association with identifiable causes (chiefly connective tissue disease, environmental exposures, and drugs) or as idiopathic conditions . One complication of ILD is acute respiratory failure (ARF), which may develop as the inaugural manifestation or as an acute exacerbation of chronic ILD. ILD-associated ARF may require admission to the intensive care unit (ICU). Little is known about the clinical features and outcomes of ARF complicating ILD. Patients may meet Berlin’s criteria for acute respiratory distress syndrome (ARDS) , but whether they constitute a specific subset of ARDS is unclear. Most studies of ARDS excluded patients with previously diagnosed chronic ILD , , , and patients with ARDS inaugurating ILD were not studied separately . ARF/ARDS complicating ILD may differ from other forms of ARF/ARDS regarding the response to corticosteroids and the outcome. Furthermore, ILD is responsible for an increase in lung stiffness that may increase the risk of ventilator-induced injury compared to other causes of ARF or ARDS. High positive end-expiratory pressure (PEEP) was associated with increased mortality in a retrospective cohort study of patients with ILD .
Ascribing inaugural ARF to ILD may be challenging. The early diagnosis of ILD is crucial to improve outcome prediction, choose optimal ventilator settings, and assess the appropriateness of specific treatments such as corticosteroids or immunosuppressants. Knowledge of outcome predictors that can be assessed early after ICU admission would help to guide the use of specific treatments. However, the low incidence of ILD-associated ARF requiring ICU admission has been a major obstacle to research into outcome predictors and treatment optimisation. In two studies, mortality was high, from 47% overall to 89.7% among patients who required invasive mechanical ventilation , . As a result, intensivists may be reluctant to admit patients with ILD and ARF .
We conducted an 11-year retrospective study of patients admitted to our ICU with ILD-associated ARF. Our objectives were to describe the clinical and imaging study features, ventilator settings, and outcomes; and to identify early predictors of hospital mortality, long-term mortality, and corticosteroid responsiveness. In addition, in the mechanically ventilated patients, we evaluated correlations linking ventilator settings, computed tomography (CT) findings, and outcomes.
Patients and Methods
We retrospectively studied patients with ILD-associated ARF admitted to the ICU of a 650-bed tertiary hospital (Saint-Louis University Hospital, Paris, France) whose pulmonology department is highly specialized in ILD. We included 114 consecutive adults with ILD admitted to our ICU between January 2002 and March 2013. ICU admission policies did not change during this period. Patients were identified retrospectively by searching the electronic ICU database. After ICU discharge, all patients were managed at our hospital, usually by a pulmonologist. The study was approved by the ethics committee of the French Society for Intensive Care (Société de Réanimation de Langue Française) (CE SRLF 13–37). Patient records were anonymized and de-identified prior to analysis.
We included patients with ILD who had ARF requiring high-dose oxygen therapy (>6 L/min), non-invasive ventilation (NIV) or, invasive mechanical ventilation (IMV). Patients had either previously diagnosed ILD meeting American Thoracic Society/European Respiratory Society (ATS/ERS) International Multidisciplinary Consensus Classification of the Idiopathic Interstitial Pneumonias ,  or ILD diagnosed after ICU admission. ILD was either idiopathic or related to known causes (e.g., drugs, radiation, or connective tissue disease).
Patients with ILD were categorised into subtypes according to the ATS/ERS International Multidisciplinary Consensus Classification of the Idiopathic Interstitial Pneumonias  based on an extensive chart review by a panel composed of a radiologist, two pulmonologists, and an intensivist. A careful clinical exam and tests for connective tissue diseases have been performed routinely by intensivists and pulmonologists in charge of these patients to exclude an acute exacerbation of previously undiagnosed dermatomyositis, polymyositis, rheumatoid arthritis, primary Sjögren syndrome or systemic lupus erythematosus. Based on clinical examination, a consultation with a specialist in internal medicine and/or a rheumatologist (especially when articular manifestations were present) was obtained. We typically obtained serum muscle enzymes (eg. alanine aminotransferase, aspartate aminotransferase, creatine kinase), an antinuclear antibody test, a rheumatoid factor and an anti-Jo-1 antibody. If these tests were negative, additional tests for antibodies to RNA synthetases and signal recognition particle (anti-SRP) have been performed.
Acute exacerbations were defined based on criteria reported by Akira et al.  : subjective worsening of dyspnoea within the last month; new ground-glass opacities or consolidation by chest radiography or high-resolution computed tomography (CT); hypoxemia with a >10 mmHg decline in partial pressure of oxygen (PaO2); no evidence of lung infection by respiratory cultures or serological tests; and no clinical evidence of pulmonary embolism, congestive heart failure, or pneumothorax.
Hospital mortality was the primary outcome. We also assessed 1-year mortality rates, ICU and hospital lengths of stay, and response to corticosteroids. Responsiveness to corticosteroids was defined as an increase in the ratio of PaO2 over fraction of inspired oxygen (FiO2) ratio to more than 100 mmHg within 1 week of initiating high-dose corticosteroid therapy, as previously described , . CT evidence of lung fibrosis was defined as the visualisation of traction bronchiectasis and/or honeycombing.
ARDS was defined using Berlin criteria  as ARF not fully explained by heart failure or fluid overload as judged by the treating physician, bilateral opacities consistent with pulmonary oedema by chest radiography or CT, and onset within 1 week after a known clinical insult or new/worsening respiratory symptoms. PaO2/FiO2 between 201 and 300 mmHg defined mild ARDS, 101 and 200 mmHg moderate ARDS, and PaO2/FiO2 less than or equal to 100 mmHg severe ARDS; these PaO2/FiO2 values were with a PEEP level at least of 5 cm H2O.
Pre-capillary pulmonary hypertension (estimated by ultrasonography) was defined by systolic pulmonary artery pressure > 35 mmHg without increased left ventricular filling pressure.
The data in Tables 1, 2 and 3 were abstracted from the medical records. The clinical, laboratory, and imaging data for each patient were reviewed by three intensivists, two of whom are also pulmonologists. Careful attention was given to recording mechanical ventilation settings including tidal volume (VT, adjusted for lung size estimated from predicted body weight), respiratory rate, peak airway pressure, plateau airway pressure, PEEP, and FiO2. We also recorded the use of prone positioning, inhaled nitric oxide, and neuromuscular blockers. Data on fluid balance, the Sequential Organ Failure Assessment (SOFA) score  on the first ICU day, and treatment limitation decisions were recorded.
Results are described as median and interquartile range (IQR) for quantitative variables and frequencies and percentages for qualitative variables. To determine the response to stepwise changes in PEEP, we compared the average peak and plateau airway pressures (when available), respiratory system compliance, expired VT, and PaO2/FiO2 before and after PEEP increases >4 cmH2O at PEEP levels >8 cmH2O. Multivariable Cox proportional-hazards regression was used to examine the 12-month survival mortality in patients with and without ILD-related pulmonary hypertension or CT evidence of lung fibrosis. We plotted Kaplan-Meier curves of 1-year survival for both these subgroups. We assessed the sensitivity and specificity of proteinuria, arthralgia, and skin rash for diagnosing connective tissue disease with their 95% confidence interval (exact binomial test). Possible predictors of hospital mortality and 1-year mortality were identified with the use of univariable logistic regression. Covariates available at ICU admission and associated with p values lower than 0.2 by univariable analysis or deemed clinically relevant were included in a multivariable logistic regression selection process. Given the number of in-hospital deaths, a maximum of four covariates was allowed in the tested models. Bootstrapping and data imputation were used to ensure the robustness of the model selection procedure: 30 datasets were generated via multiple imputations by chained equation. For each dataset, 200 bootstrap samples were drawn by random sampling with replacement. A forward/backward stepwise Bayesian information criterion (BIC)-based selection was repeated in the 6000 bootstrapped datasets . The final model was the one with p<0.05 for all covariates selected in most bootstrap samples. We checked that omitting each of the selected variables induced no significant increase in the likelihood of hospital mortality and the model’s calibration was tested by the le Cessie–van Houwelingen goodness-of-fit test . Odds ratios (ORs) and their 95% confidential intervals (CI) were calculated from the original non-imputed dataset. Statistical analysis was performed with R version 2.15.1 (R Development Core Team 2011; R Foundation for Statistical Computing, Vienna, Austria).
Patient characteristics and outcomes
Of the 114 patients with ILD admitted for ARF during the 11-year study period, 31 had cardiogenic pulmonary oedema and were excluded (Figure 1), leaving 83 patients for the study. Table 1 lists their main baseline characteristics. All patients had severe hypoxemia and diffuse pulmonary infiltrates by chest radiography; none had evidence of left heart failure. Berlin criteria for ARDS were met in 60 (73%) patients, most of whom had severe ARDS (Table 2). Invasive mechanical ventilation (IMV) was required in 50 (60%) patients and vasopressors in 42 (50%) (Table 2). Acute kidney injury developed during the ICU stay in 42 (50%) patients (Table 1).
Of the 83 patients, 34 (41%) had previously diagnosed ILD and 49 (59%) had ILD diagnosed after ICU admission. Six (0.07%) patients were on low-dose supplemental oxygen prior to hospitalization. Table 1 lists the main causes of ILD. Connective tissue disease was the leading cause (33.7% of patients). A proteinuria/creatinuria ratio >30 mg/mmoL was highly sensitive for detecting connective tissue disease-associated ILD (sensitivity of 0.88, (0.69–0.97); specificity of 0.79 (0.64–0.89)). Skin rash and arthralgia were highly specific symptoms of connective tissue disease-associated ILD in ARF-related ILD (specificity of 0.98, 0.89–1.00; and of 0.84, 0.71–0.92; respectively).
Table 3 reports the results of the diagnostic investigations. CT showed ground-glass attenuation in 70% of patients and fibrosis in 35.8%. No correlations were found between CT features and broncho-alveolar lavage (BAL) fluid characteristics. Histological data were available for only 11 patients.
High-dose corticosteroid therapy was used during the ICU stay in 54 (65%) patients. Twenty-three (28%) patients required low-dose corticosteroids prior to hospitalization. Median time from hospital admission to high-dose corticosteroid therapy initiation was 5 (2–12) days. Of the 54 patients, 32 (59%) met our criteria for responsiveness. Among the 18 patients on IMV who responded to high-dose corticosteroid therapy, all but 2 were successfully extubated within a few weeks; the remaining 2 patients improved initially then experienced fatal complications. Median ICU-length of stay was 8 (3, 14) days. Hospital mortality was 41% and 1-year mortality 53.7%.
Factors associated with hospital mortality and 1-year mortality
By univariable analysis, age, pre-capillary pulmonary hypertension, CT fibrosis, SOFA score, acute kidney injury, shock and response to high-dose corticosteroids yielded p values <0.005 for association with hospital mortality. Mortality rates were significantly higher in the subgroups with acute-on-chronic idiopathic ILD exacerbation or toxic ILD than in the subgroups with connective tissue disease-associated ILD or acute idiopathic interstitial pneumonia (Table 4).
Three factors were independently associated with hospital mortality by multivariable analysis: pre-capillary pulmonary hypertension (OR, 4.55; 95%CI (1.20–17.33)), traction bronchiectasies and/or honeycombing on CT scan (OR, 7.68; 95%CI (1.78–33.22)), and acute kidney injury (OR, 10.60; 95%CI (2.25–49.97)) (Table 4).
Multivariate analysis of variables associated with 1-year mortality showed that respiratory SOFA score (OR, 2.20; 95%CI (1.01–4.76)), performance status ≥2 (OR, 4.80; 95%CI (1.10–20.91)), traction bronchiectasies and/or honey combing (OR, 6.30; 95%CI (1.50–26.52)) and mechanical ventilation (OR, 5.18; 95%CI (1.18–22.75)) were associated with poorer survival (Table S1).
After checking the proportional hazards assumption, the multivariable Cox regression identified ILD-related pulmonary hypertension and CT fibrosis as factors associated with a higher 12-month mortality: hazards ratios (HR): 3.21 (95%CI, 1.45–7.11; p = 0.004) and 2.36 (95%CI, 1.05–5.32; p = 0.038) respectively. The respective Kaplan-Meier curves are plotted figures 2a and 2b respectively for the first 12 months.
Factors associated with responsiveness to high-dose corticosteroids
By univariable analysis, variables yielding p values <0.05 for the association with responsiveness to high-dose corticosteroids were age, CT fibrosis, BAL fluid lymphocytosis, and acute kidney injury. By multivariable analysis, only two factors were independently associated with non-responsiveness to high-dose corticosteroids: CT fibrosis (OR, 0.03; 95%CI (0.005–0.21)) and longer time from hospital admission to first corticosteroid bolus (OR, 0.88/day; 95%CI (0.79–0.97)) (Table 5).
Ventilator settings and effect of PEEP titration in patients given invasive mechanical ventilation –IMV)
Ventilator settings in patients managed with IMV are reported in Table S2. Prone positioning was used in only 11 patients, which precluded a valid statistical analysis of this variable.
PEEP titration with PEEP steps >4 cmH2O was performed in 37 patients during the first 24 h of mechanical ventilation. Increases in plateau pressure and peak airway pressure during PEEP titration correlated positively with ICU mortality, whereas increase in PaO2/FiO2 ratio correlated negatively with ICU mortality (Figure 3). Increases in plateau pressure and peak airway pressure during PEEP titration correlated positively with CT fibrosis (Figure 3). Of note, the maximum PEEP applied in patients with and without CT fibrosis were not different (respectively, median PEEP 11 cmH20 IQR (7.75–12) versus 10 cmH20 IQR (7.25–13.5), p = 0.81).
Right column: correlations linking variations in peak airway pressure (ΔPpeak), plateau pressure (ΔPplat), and PaO2/FiO2 (ΔPF) before and after PEEP titration to ICU mortality. Left column: correlations linking variations in peak airway pressure (ΔPpeak), plateau pressure (ΔPplat), and PaO2/FiO2 (ΔPF) before and after PEEP titration to pulmonary fibrosis by computed tomography.
We found high hospital and 1-year mortality rates, of 41% and 53.7%, respectively, in patients admitted to our ICU for ARF complicating ILD, among whom 73% met criteria for ARDS and 60% received invasive mechanical ventilation. Fifty-nine percent of patients with ARF complicating ILD had never been previously diagnosed prior to their admission at ICU. High-dose corticosteroid therapy was administered in the ICU in two-thirds of patients, among whom 59% responded to this treatment. Fibrosis by CT was associated with hospital mortality, 1-year mortality, and failure to respond to high-dose corticosteroid therapy. In addition, acute kidney injury and pulmonary hypertension predicted hospital mortality. Delayed administration of the first corticosteroid bolus decreased the probability of obtaining a response.
Our hospital and 1-year mortality rates are consistent with a previous retrospective study of 94 patients with ILD and ARF requiring IMV in which mortality rates were 53% at hospital discharge and 59% after 1 year . We did not have any information on the triage process to ICU admission and we were therefore unable to determine whether some patients with severe ILD were denied ICU admission. If such was the case, then our mortality rates would constitute an underestimation of true mortality rates in ARF complicating ILD.
More than half of our patients were not known to have ILD at ICU admission. Diagnosing ILD in patients with ARF is a major diagnostic challenge. Crackles on auscultation and pulmonary infiltrates on chest radiographs are nonspecific signs that do not separate patients with ILD from those with other forms of ARDS. A careful assessment of extra-pulmonary signs is crucial. In our study, 98% of the patients with skin lesions at ICU admission had connective tissue disease. Proteinuria was highly sensitive and a skin rash highly specific for connective tissue disease in our population of patients with ILD.
High-resolution CT is more sensitive than chest radiography for diagnosing and classifying ILD , . We found that CT evidence of fibrosis, defined as traction bronchiectasis and/or honeycombing, was associated with hospital and 1-year mortality rates. In addition, in keeping with an earlier study , fibrosis by CT predicted failure to respond to high-dose corticosteroid therapy. Traction bronchiectasis and honeycombing indicate advanced histopathological alterations  associated with increased lung stiffness, poorer alveolar-capillary gas exchange and greater vulnerability of the lung if IMV is used.
Another predictor of hospital mortality in our cohort was pre-capillary pulmonary hypertension indicating right ventricular dysfunction at ICU admission. No patient in our cohort was treated with vasodilator or endothelial antagonist therapy specifically for pulmonary hypertension. Pulmonary hypertension in patients with ARF complicating ILD can be related to multiple factors including hypoxia, hypercapnia, mediator-induced vasoconstriction, vascular compression by oedema and fibrosis, and vascular wall remodelling. In mechanically ventilated patients, increased intra-thoracic pressures adversely influence the right ventricular afterload , , and pulmonary hypertension is of adverse prognostic significance in patients with ARDS  and/or idiopathic pulmonary fibrosis . Whether pulmonary hypertension in ILD-associated ARF is directly involved in the occurrence of organ failures or merely reflects a more advanced stage of the lung disease remains to be elucidated.
In our cohort, BAL was performed in 64% of patients. BAL fluid cytology provides useful diagnostic information and may help to rule out active infection . The treatment of ILD targets the inflammatory process, since the fibrosis is irreversible. The presence of active alveolar and interstitial inflammation suggests that high-dose corticosteroid and/or immunosuppressive treatment may prove beneficial. Lymphocytosis in the BAL fluid may indicate early-stage ILD with active inflammation. However, in our multivariable analysis BAL fluid lymphocytosis was not associated with responsiveness to high-dose corticosteroid therapy. According to current recommendations about BAL in patients with ILD overall, the BAL fluid pattern provides useful diagnostic information but does not predict the prognosis  or treatment response , , . In our study, delayed initiation of high-dose corticosteroid therapy was independently associated with a lower response rate. This finding emphasises the need for an early diagnosis of ILD in patients admitted to the ICU with ARF.
Among our patients, 60% received IMV. Larger plateau pressure increases during PEEP titration correlated strongly with CT fibrosis and ICU mortality. In a retrospective study of ventilator settings in 53 medical and 41 postsurgical ICU patients with ILD and ARF, high PEEP was independently associated with higher mortality . High PEEP failed to improve oxygenation and was associated with lung overdistension. Fibrosis results in stiffness of the lung , which increases the risk of ventilator-induced lung injury. In patients with ARDS and a small percentage of recruitable lung parenchyma, high PEEP was not beneficial . In ILD at the stage of lung fibrosis, the proportion of recruitable lung is probably small and the risk of overdistension high, as suggested by the larger plateau pressure increases seen in patients with CT evidence of fibrosis in our study. Thus, our results indicate that caution is in order when considering the applicability of data on PEEP settings in the overall population of ARDS patients ,  to patients with ILD-associated ARDS. In this last population, the use of high PEEP levels should be viewed with circumspection, particularly in patients with CT fibrosis and/or pulmonary hypertension. Similarly, recruitment manoeuvres may increase the risk of ventilator-induced lung injury in ILD-associated ARF.
Our study has several limitations. We used a single-centre retrospective design. Data on triage to ICU admission were not available, and our study may have missed patients with severe ILD who were considered too ill to benefit from ICU admission. Lung histopathological data were obtained in the ICU in only 13% of our patients, and some patients who had ILD diagnosed in the ICU may therefore have been misclassified. This source of bias was minimised in our study by a detailed review of all cases by a radiologist, two pulmonologists, and an intensivist, who used ATS guidelines . The low rate of open lung biopsy probably reflects concern among intensivists about the risk of increased morbidity and mortality rates associated with this procedure. Moreover, the usefulness of urgent histopathological documentation has been challenged .
Our work has several important clinical implications. First, a careful physical examination for skin rash, arthralgia, and proteinuria may help to identify connective tissue disease, which is a common cause of ILD. Second, pulmonary hypertension and CT signs of fibrosis predict poor survival in all patterns of ILD with ARF. Third, whether a high lymphocyte count in BAL fluid predicts a good response to corticosteroids remains unclear. Fourth, when high-dose corticosteroid therapy is indicated, it should be started promptly to maximise the likelihood of a response. Finally, in patients managed with IMV, high PEEP levels may be harmful, most notably in patients with lung fibrosis.
Conceived and designed the experiments: LZ VL EA GL BS. Analyzed the data: NL LZ VL EA. Contributed to the writing of the manuscript: LZ EA.
- 1. American Thoracic Society/European Respiratory Society International Multidisciplinary Consensus Classification of the Idiopathic Interstitial Pneumonias. This joint statement of the American Thoracic Society (ATS), and the European Respiratory Society (ERS) was adopted by the ATS board of directors, June 2001 and by the ERS Executive Committee, June 2001. Am J Respir Crit Care Med 165: 277–304.
- 2. Ranieri VM, Rubenfeld GD, Thompson BT, Ferguson ND, Caldwell E, et al. (2012) Acute respiratory distress syndrome: the Berlin Definition. JAMA 307: 2526–2533.
- 3. Amato MB, Barbas CS, Medeiros DM, Magaldi RB, Schettino GP, et al. (1998) Effect of a protective-ventilation strategy on mortality in the acute respiratory distress syndrome. N Engl J Med 338: 347–354.
- 4. Ventilation with lower tidal volumes as compared with traditional tidal volumes for acute lung injury and the acute respiratory distress syndrome. The Acute Respiratory Distress Syndrome Network. N Engl J Med 342: 1301–1308.
- 5. Guerin C, Reignier J, Richard JC, Beuret P, Gacouin A, et al. (2013) Prone positioning in severe acute respiratory distress syndrome. N Engl J Med 368: 2159–2168.
- 6. Thille AW, Esteban A, Fernandez-Segoviano P, Rodriguez JM, Aramburu JA, et al. (2013) Comparison of the Berlin definition for acute respiratory distress syndrome with autopsy. Am J Respir Crit Care Med 187: 761–767.
- 7. Fernandez-Perez ER, Yilmaz M, Jenad H, Daniels CE, Ryu JH, et al. (2008) Ventilator settings and outcome of respiratory failure in chronic interstitial lung disease. Chest 133: 1113–1119.
- 8. Gungor G, Tatar D, Salturk C, Cimen P, Karakurt Z, et al. (2013) Why do patients with interstitial lung diseases fail in the ICU? a 2-center cohort study. Respir Care 58: 525–531.
- 9. Raghu G, Collard HR, Egan JJ, Martinez FJ, Behr J, et al. (2011) An official ATS/ERS/JRS/ALAT statement: idiopathic pulmonary fibrosis: evidence-based guidelines for diagnosis and management. Am J Respir Crit Care Med 183: 788–824.
- 10. Akira M, Hamada H, Sakatani M, Kobayashi C, Nishioka M, et al. (1997) CT findings during phase of accelerated deterioration in patients with idiopathic pulmonary fibrosis. AJR Am J Roentgenol 168: 79–83.
- 11. Meduri GU, Golden E, Freire AX, Taylor E, Zaman M, et al. (2007) Methylprednisolone infusion in early severe ARDS: results of a randomized controlled trial. Chest 131: 954–963.
- 12. Meduri GU, Headley AS, Golden E, Carson SJ, Umberger RA, et al. (1998) Effect of prolonged methylprednisolone therapy in unresolving acute respiratory distress syndrome: a randomized controlled trial. JAMA 280: 159–165.
- 13. Vincent JL, Moreno R, Takala J, Willatts S, De Mendonca A, et al. (1996) The SOFA (Sepsis-related Organ Failure Assessment) score to describe organ dysfunction/failure. On behalf of the Working Group on Sepsis-Related Problems of the European Society of Intensive Care Medicine. Intensive Care Med 22: 707–710.
- 14. Schwarz G (1978) Estimating the Dimension of a Model Ann Statist. 6: 461–464.
- 15. le Cessie S, van Houwelingen JC (1991) A Goodness-of-Fit Test for Binary Regression Models, Based on Smoothing Methods. Biometrics 47: 1267–1282.
- 16. Hodnett PA, Naidich DP (2013) Fibrosing Interstitial Lung Disease. A Practical High-Resolution Computed Tomography-based Approach to Diagnosis and Management and a Review of the Literature. Am J Respir Crit Care Med 188: 141–149.
- 17. Ichikado K, Suga M, Muller NL, Taniguchi H, Kondoh Y, et al. (2002) Acute interstitial pneumonia: comparison of high-resolution computed tomography findings between survivors and nonsurvivors. Am J Respir Crit Care Med 165: 1551–1556.
- 18. Ichikado K, Johkoh T, Ikezoe J, Takeuchi N, Kohno N, et al. (1997) Acute interstitial pneumonia: high-resolution CT findings correlated with pathology. AJR Am J Roentgenol 168: 333–338.
- 19. Vieillard-Baron A, Schmitt JM, Augarde R, Fellahi JL, Prin S, et al. (2001) Acute cor pulmonale in acute respiratory distress syndrome submitted to protective ventilation: incidence, clinical implications, and prognosis. Crit Care Med 29: 1551–1555.
- 20. Moloney ED, Evans TW (2003) Pathophysiology and pharmacological treatment of pulmonary hypertension in acute respiratory distress syndrome. Eur Respir J 21: 720–727.
- 21. Bull TM, Clark B, McFann K, Moss M (2010) Pulmonary vascular dysfunction is associated with poor outcomes in patients with acute lung injury. Am J Respir Crit Care Med 182: 1123–1128.
- 22. Judge EP, Fabre A, Adamali HI, Egan JJ (2012) Acute exacerbations and pulmonary hypertension in advanced idiopathic pulmonary fibrosis. Eur Respir J 40: 93–100.
- 23. Meyer KC, Raghu G, Baughman RP, Brown KK, Costabel U, et al. (2012) An official American Thoracic Society clinical practice guideline: the clinical utility of bronchoalveolar lavage cellular analysis in interstitial lung disease. Am J Respir Crit Care Med 185: 1004–1014.
- 24. Strange C, Bolster MB, Roth MD, Silver RM, Theodore A, et al. (2008) Bronchoalveolar lavage and response to cyclophosphamide in scleroderma interstitial lung disease. Am J Respir Crit Care Med 177: 91–98.
- 25. Boomars KA, Wagenaar SS, Mulder PG, van Velzen-Blad H, van den Bosch JM (1995) Relationship between cells obtained by bronchoalveolar lavage and survival in idiopathic pulmonary fibrosis. Thorax 50: 1087–1092.
- 26. Fireman E, Vardinon N, Burke M, Spizer S, Levin S, et al. (1998) Predictive value of response to treatment of T-lymphocyte subpopulations in idiopathic pulmonary fibrosis. Eur Respir J 11: 706–711.
- 27. Nava S, Rubini F (1999) Lung and chest wall mechanics in ventilated patients with end stage idiopathic pulmonary fibrosis. Thorax 54: 390–395.
- 28. Gattinoni L, Caironi P, Cressoni M, Chiumello D, Ranieri VM, et al. (2006) Lung recruitment in patients with the acute respiratory distress syndrome. N Engl J Med 354: 1775–1786.
- 29. Richard JC, Maggiore SM, Jonson B, Mancebo J, Lemaire F, et al. (2001) Influence of tidal volume on alveolar recruitment. Respective role of PEEP and a recruitment maneuver. Am J Respir Crit Care Med 163: 1609–1613.
- 30. Mercat A, Richard JC, Vielle B, Jaber S, Osman D, et al. (2008) Positive end-expiratory pressure setting in adults with acute lung injury and acute respiratory distress syndrome: a randomized controlled trial. JAMA 299: 646–655.
- 31. Chuang ML, Lin IF, Tsai YH, Vintch JR, Pang LC (2003) The utility of open lung biopsy in patients with diffuse pulmonary infiltrates as related to respiratory distress, its impact on decision making by urgent intervention, and the diagnostic accuracy based on the biopsy location. J Intensive Care Med 18: 21–28.