Skip to main content
Advertisement
Browse Subject Areas
?

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

  • Loading metrics

Late Cretaceous Aquatic Plant World in Patagonia, Argentina

  • N. Rubén Cúneo ,

    rcuneo@mef.org.ar

    Affiliation Museo Paleontológico Egidio Feruglio - CONICET. Trelew, Chubut, Argentina

  • María A. Gandolfo,

    Affiliation L. H. Bailey Hortorium, Plant Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, New York, United States of America

  • María C. Zamaloa,

    Affiliation Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina

  • Elizabeth Hermsen

    Affiliation Department of Environmental and Plant Biology, Ohio University, Athens, Ohio, United States of America

Abstract

In this contribution, we describe latest Cretaceous aquatic plant communities from the La Colonia Formation, Patagonia, Argentina, based on their taxonomic components and paleoecological attributes. The La Colonia Formation is a geological unit deposited during a Maastrichtian-Danian transgressive episode of the South Atlantic Ocean. This event resulted in the deposition of a series of fine-grained sediments associated with lagoon systems occurring along irregular coastal plains in northern Patagonia. These deposits preserved a diverse biota, including aquatic and terrestrial plants and animals. The aquatic macrophytes can be broadly divided into two groups: free-floating and rooted, the latter with emergent or floating leaves. Free-floating macrophytes include ferns in Salviniaceae (Azolla and Paleoazolla) and a monocot (Araceae). Floating microphytes include green algae (Botryoccocus, Pediastrum and Zygnemataceae). Among the rooted components, marsileaceous water ferns (including Regnellidium and an extinct form) and the eudicot angiosperm Nelumbo (Nelumbonaceae) are the dominant groups. Terrestrial plants occurring in the vegetation surrounding the lagoons include monocots (palms and Typhaceae), ferns with affinities to Dicksoniaceae, conifers, and dicots. A reconstruction of the aquatic plant paleocommuniy is provided based on the distribution of the fossils along a freshwater horizon within the La Colonia Formation. This contribution constitutes the first reconstruction of a Cretaceous aquatic habitat for southern South America.

Introduction

There are currently about 2,600 species of vascular aquatic macrophytes distributed among 87 families and 407 genera [1]; these plants inhabit all types of wetland environments and are found throughout the world [2], although they are most diverse in the tropics, a pattern that is particularly pronounced for aquatic angiosperms [3]. Today, vascular aquatic macrophytes are represented by lycophytes (Isoetes), ferns (Salviniales or Hydropteridales) [4][5], and angiosperms (e.g., Nymphaeales, Nelumbonaceae, and Ceratophyllum, to name a few) while non-vascular macrophytes include macroalgae (e.g., Chara, Nitella), and bryophytes (certain liverworts and mosses) [6]. The fossil record suggests that aquatic ecosystems were conquered by vascular macrophytes in the late Early Cretaceous [7]. Heterosporous water ferns with affinities to the order Salviniales (or Hydropteridales) [4][5] first appeared in the latest Jurassic to earliest Cretaceous [8][9][10][11], whereas the earliest aquatic angiosperms, such as Archaefructus, Proteaphyllum, and ranunculaceous species, appeared in the mid-Early Cretaceous (Barremian-Aptian) [7][12]. A greater diversity of aquatic angiosperms, such as Nymphaeales [13][14] and some monocots [15][16][17], appeared later in the Early Cretaceous, when the first unequivocal records of green algae (Hydrodictyaceae, Scenedesmaceae, Coelastraceae and Chlorococcaceae) are also recorded [18][19][20].

By the Late Cretaceous, freshwater angiosperms were the dominants in aquatic assemblages. For example, aquatic communities documented from Europe [21] include the basal angiosperm Nymphaeites (Nymphaeaceae), monocots such as Typhaceae, and the eudicots Nelumbites (Nelumbonaceae) and Palaeoaldrovanda (Droseraceae), as well as halophytic aquatic plants (although in some cases the affinities of the latter have been questioned) [8]. In Mongolia [22], several Maastrichtian localities of the Nemegt Formation bear aquatic plants that comprise three distinct assemblages: 1- a pondweed assemblage dominated by Potamogeton-like plants associated with an isoetalean component; 2- a nymphaealean assemblage; and 3- an assemblage dominated by duckweeds (Araceae subfamily Lemnoideae). These three assemblages occurred in oxbow lake deposits associated with fluvial systems under a temperature regime that was probably subtropical with a monsoonal dry season. Cretaceous and Paleocene aquatic plant communities from western North America and northeastern Asia [23][24][25][26][27] resemble modern communities, and include common elements such as Nelumbites (Proteales), Quereuxia (an angiosperm of unknown affinities), Cobbania and Limnobiophyllum (monocots in Araceae), along with extinct aquatic ferns (Hydropteris in Salviniales) [5].

Comparatively little is known about the evolution of aquatic macrophytes in the Southern Hemisphere, particularly from macrofossil records. Recently, however, a series of contributions describing aquatic macrophytes from the Maastrichtian La Colonia Formation of Patagonia, Argentina, have been published [28][29][30][31][32][33]. The La Colonia Formation paleoflora, currently documented from three localities that yield macrofossils, is interpreted as preserved in situ or after minimal transport given the excellent preservation of delicate plant organs. This interpretation is also supported by the presence of a highly diverse green microalgal component. In this contribution, we summarize recently published and new findings on the composition and environment of the La Colonia Formation paleofloras. This constitutes the first detailed description and ecological interpretation of a latest Cretaceous aquatic plant community from southern South America.

Materials and Methods

Macrofossils and palynological samples were collected from several localities of the La Colonia Formation, which outcrops in the central part of northern Chubut Province in Patagonia, Argentina (Fig. 1). Based on the characteristics of its paleobiota, which includes plant macro- and microfossils, dinoflagellates, invertebrates, and vertebrates, the formation is thought to have been deposited during the latest Cretaceous into possibly the earliest Danian [29][34][35][36]. For this contribution, we examined two stratigraphic sections from a plant horizon that can be traced for a distance of approximately 10 km through the La Colonia Formation (Figs. 1, 2). These two sections include localities that yield plant macrofossils: Cerro Bosta and Cañadón del Irupé/Quebrada del Helecho (Fig. 2). The sections were measured from base to top and their sedimentological attributes—including lithologies, sedimentary structures, and fossil content–were noted. Macrofossils and microfossils were identified and their dominance was determined qualitatively. A total of five palynological samples per locality were analyzed from the same horizons as the macrofossils, and they were processed following standard palynological techniques.

thumbnail
Figure 1. Map showing the location of the outcrops of the La Colonia Formation examined for this study in the Cerro Buitre and Arroyo Mirasol Chico areas.

Modified after (101).

https://doi.org/10.1371/journal.pone.0104749.g001

thumbnail
Figure 2. Stratigraphic sections of the La Colonia Formation at the Cerro Bosta locality (a) and Cañadón del Irupé/Quebrada del Helecho localities (b).

Note distribution of lagoon facies and fresh water horizon after correlation of aquatic plants.

https://doi.org/10.1371/journal.pone.0104749.g002

No permits were required for the described study. Since the current study is based on specimens previously collected, no particular permit was necessary. This is clearly established in the by-laws of the Chubut Province legislation dealing with fossil protection (Law Number 3559). All macrofossil specimens are housed in the Museo Paleontológico Egidio Feruglio (MEF) Paleobotanical Collection (MPEF-Pb) in Trelew, Chubut, Argentina; palynological samples are also housed in the MEF Palynological collection (MPEF-Mz Palin) and at the Laboratorio de Paleobotánica y Palinología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina (BAFCB). All specimens and data supporting this contribution is freely available throughout public repository as indicated above at www.mef.org.ar/index.php?option = com_content&view = article&id0102&ltemid085&lang = es.

Geology and sedimentology of the La Colonia Formation

Background.

The La Colonia Formation is a sedimentary accumulation that outcrops at the southeastern edge of the Somuncurá Massif in northern Patagonia, Argentina [37] (Fig. 1). The Somuncurá Massif (or Plateau) is a structural unit composed of Paleozoic metamorphic and igneous complexes, which are the source rocks for clastic Late Cretaceous/early Paleogene sedimentary deposits occurring in the region. A major transgression caused by subsidence of the south Atlantic margin took place during the Late Cretaceous, which gave rise to extensive shallow epicontinental seas defined as Patagonian Platforms [35][38]. These seas covered most of the Patagonian region and represented the last episode of a tectonic foreland stage [39]. Thus, a series of Late Cretaceous coastal plain sedimentary deposits representing estuaries, deltas, and lagoon/barrier complexes accumulated [36][40].

Deposits of the La Colonia Formation have a maximum thickness of approximately 240 m [37]. They are underlain by late Early to Late Cretaceous sediments of the Chubut Group or the Puntudo Chico Formation [41], and are exposed in the Cerro Buitre/Mirasol Chico Creek area (Fig. 1), and towards the NE in the Telsen area over the volcanics of the Jurassic Marifil Formation [36]. This Jurassic unit seems to have played an important role in shaping the topography of the landscape during the latest Cretaceous transgression [36], probably as part of the Atlantic Patagonian Dorsal [38]. The La Colonia Formation is overlain by basalts of the El Buitre Formation (Paleocene) and the informally named “Baibian Beds” (probably Eocene to early Oligocene) to the southwest [42]. Above these is a basaltic intrusion (Somuncurá Formation), which is succeeded by the classic Oligocene-early Miocene deposits of the Sarmiento Formation. The whole sequence in this area is crowned by Neogene basalts and Quaternary sedimentary deposits (Tehuelches Pebbles).

The sedimentology of the La Colonia Formation has been characterized based on data collected from several localities. Three lithofacies associations were initially described along the eastern slope of the La Colonia Formation in the proximity of the Cerro Buitre area (Fig. 1) [34]. The lowest association is composed of coarse-grained sandy to conglomeratic cross-bedded deposits up to 16 m thick and was initially interpreted as a terrestrial setting drained by low- to moderately sinuous fluvial channels [34]. The middle and thickest association is represented by fine-grained deposits and includes three facies (laminated mudstones, pelite-fine sandstones, and banded siltstones and claystones) thought to have been deposited on coastal plains. The upper association is a single facies consisting of laminated claystones deposited in an intertidal zone.Two of the three facies (pelite-fine sandstones and banded siltstones and claystones) have also been recognized within the middle facies association at a locality north of Cerro Buitre (Fig. 1), where the La Colonia Formation is almost 130 m in thickness [43]. Near Telsen (Fig. 1), the La Colonia Formation is much thinner (around 15 m on average) and starts with fine conglomerates that represent an erosive surface (or ravinement surface) [36][44][45] resulting from the Maastrichtian transgression. This is overlain by three sandstone facies and two fine-grained facies that define four depositional environments: 1) shoreface transitioning to off-shore, 2) tidal flats, 3) lagoons with tidal influence, and 4) lagoons without marine influence (36). The presence or absence of marine influence was determined by the presence or absence of dinoflagellates.

New sedimentological results.

Although the sedimentology of the La Colonia Formation has received detailed examination and interpretation, the major sedimentological processes responsible for the deposition of the La Colonia Formation are still poorly understood. The presence of an aquatic paleobiota in the formation is thus significant, as it is helpful for making paleoenvironmental interpretations. In this regard, we have analyzed two plant-bearing sections that correspond to the Cerro Bosta and Cañadón del Irupé/Quebrada del Helecho localities of the La Colonia Formation (Fig. 1). These sections have quite similar characteristics in terms of their thickness and in the facies associations represented. Sandy/conglomeratic coarse-grained deposits that represent the initial transgressive phase of the latest Cretaceous Atlantic Epicontinental Sea occur at the base of each section, and correlate to the erosive “key surface” (or ravinement) represented in localities to the northeast [36]. These deposits are 5 to 10 m thick and are interpreted as representing shoreface sedimentation dominated by bi-modal wave processes (Fig. 2a). This is contrary to the initial fluvial interpretation [34], but in agreement with that suggested from study of sediments in the Telsen area [36]. Notably, embayed transgressing coastlines normally lack fluvial influence in their barrier-lagoon systems [46][47].

The La Colonia Formation section measured at the Cañadón del Irupé/Quebrada del Helecho localities (Fig. 2b) is 137 m thick. It represents barrier island deposits that prograde over lagoonal deposits, creating water bodies separated from the nearshore. The barrier deposits are composed of fine- to medium-grained, laminated to cross-bedded sandstones. In some cases, laminated coarse silts to very fine sandstone deposits represent sandbars. The lagoon deposit proper is represented by fine-grained laminated to massive sediments (clays and silts), in some cases with high participation of heterolithic facies (lenticular and linsen bedding). These deposits, due to lack of terrestrial sediment supply, are associated with marine influence, in particular by washover caused by stormwaters entering the lagoon [48]. Washover and lagoonal environments tend to be preserved during transgressions [49], when organic muds are vertically accreted in a “shore zone” (Fig. 2a) [50][51]. The absence of washover channels or tidal inlets that represent connections between the open sea and the lagoons is probably due to the presence of a microtidal regime that resulted from shallow conditions along the shoreline of the Patagonian Platform sea [38]. Microtidal barrier islands tend to be long and narrow, with abundant storm wave-dominated washover deposits (Fig. 2b) that overtop the barrier [52]. This communication with the open sea is responsible for salinity changes within the lagoons, with development of aquatic plant communities during freshwater stages [53]. Therefore, coastal environments show a normal gradation from saline to freshwater conditions [54].

The section at the Cerro Bosta locality reaches 135 m in thickness (Fig. 2a) and has a well-developed shoreface facies that clearly shows a bi-modal paleocurrent pattern, suggesting tidal and wave influence. Here, barrier/washover deposits are not as evident, and most of the section appears to be dominated by the constant influence of tidal/wave processes. The exception is the horizon that yields the aquatic fossil plants, the presence of which suggests freshwater conditions. As noted for the Cañadón del Irupé/Quebrada del Helecho section, the absence of both coarse-clastic sediments and sedimentary structures indicates that the site was relatively distant from active fluvial dynamics.

In summary, the La Colonia Formation outcropping between the Cerro Bosta locality and the Cañadón del Irupé/Quebrada del Helecho, includes sedimentary deposits interpreted as clastic coastal plains bathed by shallow seas. Barrier-island/lagoon complexes developed in this region. The lagoons were protected from the sea by barrier islands, and were built by wave-dominated beach/shoreface processes normally related to low sediment supply and sea-level rise [55].

Reconstruction of the aquatic plant community of the La Colonia Fm

Modern aquatic macrophytes rarely occur as monospecific populations in freshwater systems, but instead tend to form recognizable assemblages composed of several species representing several different major life forms. These forms include free-floating macrophytes [2], also known as pleustophytes [4][56], that are not anchored to the substrate and float in the water column or on the water surface; and rooted macrophytes [2] that are anchored to the substrate with structures like rhizomes or roots and known as benthophytes [56] or, perhaps more commonly, rhizophytes [4]. Rhizomatous or rooted macrophytes can further be divided into those with leaves that are completely submerged, those with emergent leaves (borne above the surface of the water), and those with leaves floating on the water surface [2][57]. The La Colonia Formation plant community clearly includes both free-floating macrophytes as well as rooted/rhizomatous macrophytes with either emergent or floating leaves. Additionally, there is a diversity of microphytes in the form of free-floating green algae (Chlorophyta).

Free-floating microphyte component.

Green microalgae (Fig. 3), represented by members of the families Hydrodictyaceae (Pediastrum spp.) (Fig. 3 A,B), Botryococcaceae (Botryoccocus spp.) (Fig. 3C), and filamentous Zygnemataceae (Spirogyra type, Zygnema type, and Mougeotia type) (Fig. 3 D, E), comprise the microphyte component of the plant community. These planktonic algae are abundant and diverse in sediments of the localities studied, suggesting highly favorable conditions for their growth, which likely occurred in humid to wet substrates during long hydroperiods. Abundant freshwater algae indicate meso- to eutrophic conditions, strong light penetration, and small- to medium-sized bodies of water [58]. No dinoflagellate cysts were found within the microfossil samples, suggesting a lack of marine influences at these levels.

thumbnail
Figure 3. Free-floating microphyte components (chloroccocalean microalgae).

A, B (SEM): Pediastrum spp.; BAFCB meb 262. C: Botryoccocus sp. (SEM); BAFCB meb 262. D, E: Zygnemataceae spores (LM); BAFCB pm 260. Scales A, B, C = 1000 µm; D, E = 10 µm.

https://doi.org/10.1371/journal.pone.0104749.g003

Botryococcaceae colonies are today widely dispersed in temperate and tropical regions and are able to tolerate seasonally cold climates [58]. They normally live in freshwater (although sometimes in brackish water), occurring in bogs, temporary pools, ponds, and lakes. Quiet water environments are favorable to their proliferation, and they may form a thick scum on the water surface [58].

The fossil record in South America indicates that from the Late Cretaceous to the Early Eocene only the widespread Pediastrum boryanum (Hydrodictyaceae) occurs [59]. In this regard, the presence of at least three species in the La Colonia sediments denotes that the genus was well established by the late Cretaceous at high southern latitudes. Pediastrum is a freshwater planktonic coenobium that can occur in various trophic conditions [59], although theyare generally more abundant under slightly eutrophic conditions. Proliferation of Pediastrum may result in the formation of an ooze at the bottom of a lagoon, pond, or lake. Cenobia walls have a delicate sporopollenin layer that is easily broken during diagenetic processes. Nevertheless, most cenobia found in the La Colonia sediments remain complete and well preserved, denoting in situ preservation in a low energy paleoenvironment.

Zygnemataceae, one of the most common families of modern freshwater unbranched filamentous algae, typically occurs in shallow, stagnant, clean, and oxygen-rich waters where they form scums on the water surface, although a few species occur in brackish water. Members of this family produce sexual spores (zygospores) in spring under favorable conditions and also asexual resistant spores (aplanospores) that allow them to persist through periods when environmental conditions are unfavorable. Differentiation between sexual and asexual spores is not discernible in most genera but their sole presence indicates local conditions of the body of water [60][61].

Free-floating macrophytes.

The free-floating macrophyte [2] or pleustophyte [6][56] component of the La Colonia plant paleocommunity is represented by vascular plants, incudingferns and angiosperms (see Table 1). The vascular plants can be classified as acropleustophytes, or macrophytes that float on the water surface [6][56], based on their structure and comparison to related modern plants.

thumbnail
Table 1. Distribution and abundances of plant taxa represented by micro- and macrofossils at the three localities studied.

https://doi.org/10.1371/journal.pone.0104749.t001

The ferns are members of the family Salviniaceae (Order Salviniales, water ferns). Modern and fossil Salviniaceae are aquatic, free-floating, heterosporous ferns [62][63]. Salviniaceous ferns were first identified from the La Colonia Formation based on megaspores and microspore massulae assigned to Paleoazolla patagonica from the Cerro Bosta locality [28]. Paleoazolla patagonica megaspores with preserved floats and microspore massulae with characteristic glochidia bearing recurved lateral barbs were found at all three La Colonia Formation localities sampled for this study (Figs. 4 C, D). Azolla megaspores without floats preserved and microspore massulae bearing glochidia with anchor-shaped ends like those found in modern Azolla section Azolla [64][65] were also found at the Cerro Bosta and Cañadón del Irupé localities (Fig. 4 B). Additionally, Azolla-like sporophytes, including sterile and fertile material, are known from the Cañadón del Irupé locality (Figs. 5B, D) [30]. The fertile specimens bear megasporocarps and microsporocarps, the latter including smaller round bodies interpreted as in situ microspore massulae (Fig. 4 B). The spores to which the sporophyte correspond have not yet been definitively established.

thumbnail
Figure 4. Spores and pollen organs representing free-floating aquatic macrophytes.

A: Azolla-like megaspore (SEM) without floats preserved; MPEF-MEB 5505 (scale = 50 µm). B: Azolla microspore massula (SEM); BAFCB meb 263 (scale = 20 µm). C: Paleoazolla patagonica megaspore with floats preserved (SEM); MPEF-MEB 5506 (scale = 200 µm). D: Paleoazolla patagonica microspore massula (SEM); MPEF-MEB 5507 (scale = 10 µm). E: Araceae pollen grain (Pandaniidites sp.); BAFCB pm 259 (LM) (scale = 10 µm).

https://doi.org/10.1371/journal.pone.0104749.g004

thumbnail
Figure 5. Free-floating aquatic macrophyte components from the Cañadón del Irupé locality.

A, C: Lemnoid leaves; MPEF-Pb 3983. B, D: Azolla sporophytes B. Sporophyte with sporocarps (s, arrows); MPEF-Pb 5060. D: Sterile sporophytes; MPEF-Pb 5081. Scales A, C = 1 cm; B, D = 5 mm.

https://doi.org/10.1371/journal.pone.0104749.g005

Today, Azolla is frequently found in abundance floating on the surface of ponds, lakes, marshes, and slow-moving streams, in freshwater or sometimes in brackish water. It occurs in tropical to warm-temperate regions throughout the world [62][66] and has limited tolerance for frost, salinity, and drought [67][68][69]. Due the fact that Azolla does not tolerate high salinity and prefers stagnant water, it is an excellent indicator of freshwater, low energy environments [62][63][69].

Another free-floating component of this plant community is represented by leaves and pollen attributed to a lemnoid Araceae basal to the duckweed clade and definitely part of the free-floating aquatic aroids [31] (Figs. 5 A, C). The leaves belong to a newly described genus comparable to the fossil genera Limnobiophyllum and Cobbania [30][31]. They are associated with Pandaniidites-type pollen (Fig. 4E), which has also been found in situ in Limnobiophyllum anthers [70]. These fossils are found only at the Cañadón del Irupé locality [31]. Today, the Araceae are a large, mostly terrestrial tropical family with approximately 20 species adapted to the aquatic/wetland environment with low flow or stagnant shallow waters [56][71] as interpreted for the Quebrada del Irupé locality in Patagonia.

Rooted/rhizomatous macrophytes.

The rooted macrophyte [1] or benthophyte or rhizophyte [4][57] component of the La Colonia Formation flora is also represented by ferns and angiosperms (Table 1). These plants are thought to have had either emergent or floating leaves based on their structure and comparison to their nearest living relatives.

Ferns include members of the family Marsileaceae (Order Salviniales, water ferns). The fossil species Regnellidium thomas-taylorii (Fig. 6 A, B) is the only macrophyte known from two of the localities, Cerro Bosta and Quebrada del Helecho. Remains of this plant include petiolate compound leaves with two leaflets (Fig. 6B), aerenchymatous rhizomes bearing rootlets (Fig. 6 A), and a small septate sporocarp. The marsileaceous sporae dispersae Molaspora lobata (megaspores, Fig. 7A) and Crybelosporites (microspores, Fig. 7B) were recovered in abundance from the same layer bearing R. thomas-taylorii macrofossils. Mirasolita irupensis, leaves and leaflets representing an extinct form (Fig. 6C, D), co-occur with Lugiomarsiglia aquatica, representing marsileaceous sporocarps (Fig. 6D), at the Cañadón del Irupé locality; Molaspora lobata megaspores are known to occur in the same layers [33].

thumbnail
Figure 6. Rooted/rhizomatous macrophytes.

A: Regnellidium thomas-taylorii rhizomes, rootlets, petioles and leaflets; MPEF-Pb 5405. B: R. thomas-taylorii leaflets; MPEF-Pb 5446. C: Mirasolita leaflets; MPEF-Pb 5521. D: Luigimarsiglia sporocarps; MPEF-Pb 5527. E: Nelumbo puertai leaf; MPEF-Pb 864. F: Nelumbo-like associated reproductive organ; MPEF-Pb 920. Scales = 1 cm.

https://doi.org/10.1371/journal.pone.0104749.g006

thumbnail
Figure 7. Rooted/rhizomatous macrophytes represent by spores and pollen.

A: Molaspora lobata megaspore (SEM) associated with Regnellidium thomas-taylorii; MPEF-MEB 5501 (scale = 50 µm). B: Crybelosporites microspore (SEM) associated with Regnellidium thomas-taylorii; MPEF-MEB 5502 (scale = 10 µm). D: Molaspora lobata megaspore (SEM) associated with Mirasolita and Luigimarsiglia; MPEF-MEB 5503 (scale = 50 µm). D: Nelumbo-like tetracolpate-reticulate pollen grain (SEM); BAFCB pm 263 (scale = 10 µm).

https://doi.org/10.1371/journal.pone.0104749.g007

Living marsileaceaous ferns are amphibious and rhizomatous, with adventitious roots borne on the rhizome that anchors them in the substrate [72][73]. All three extant genera of Marsileaceae (Marsilea, Pilularia, and Regnellidium) are adapted to intermittently dry conditions [74][75]. Extant Marsilea and Regnellidium exhibit heterophylly, producing two to three adult leaf forms (terrestrial, floating, and submerged) depending on the availability of water when the leaves develop [76]. These leaves vary in some of their features in characteristic ways. For example, floating leaves tend to be glabrous and entire-margined, whereas terrestrial leaves tend to bear trichomes and may have terminal teeth [77][78]. The morphology of the fossil leaves suggests that they are floating or terrestrial/emergent forms. Regnellidium only grows in shallow waters or in muddy substrates that are subject to periodic flooding and the leaf petioles reach a maximum of ca. 21 cm in length [74], roughly indicating the maximum water depth in which the extant plants might occur when the leaves develop.

Sporocarps of Marsilea and Regnellidium typically develop on the leaf petioles only when the plants are exposed to air by a decrease in water level, and it is thought that sporocarp production may be stimulated by exposure of plant organs to light. Water, however, is necessary for dehiscence [76]. The leaflets of Marsilea leaves that bear sporocarps die away by the time the sporocarps are mature [76], so perhaps the Mirasolita leaves and Lugiomarsiglia sporocarps found at the Cañadón del Irupé locality were produced at different times under slightly different environmental conditions. The somewhat inexplicable paucity of marsileaceous sporocarps in the fossil record, despite the occurrence of marsileaceous ferns in habitats conducive to preservation and the durability of the sporocarps themselves, is perhaps explained by the tendency of sporocarps to float or to germinate quickly under favorable conditions [78].

The eudicot Nelumbo puertae is another macrophyte of the La Colonia Formation flora that has emergent or floating leaves. This taxon occurs at the Cañadón del Irupé locality [29][30]. The plant is represented by typical peltate, discoidal leaves (Fig. 6E) and associated infructescences (Fig. 6F). Modern Nelumbonaceae comprise two species within the genus Nelumbo. Modern Nelumbo develops horizontal rhizomes with nodal adventitious roots and tubers. Its leaves are emergent or floating, with petioles up to 2 m long. Flowers are also elevated and frequently emergent on a peduncle up to 2 m in length. The maximum lengths of the leaf petioles and flower peduncles determine the maximum depths at which this aquatic plant can grow [54][79]. Considering the size and mature condition of the nelumbonaceous remains from Cañadón del Irupé, the lagoon system at this locality likely could not have been deeper than 2 m.

Structure and organization of the aquatic paleocommunity.

Aquatic macrophyte assemblages are commonly organized along depth gradients, with macrophytes bearing emergent leaves typically dominating shallow areas, submerged macrophytes colonizing deeper sites, androoted or rhizomatous species with floating leaves inhabiting zones of intermediate depth [80]. Other physical environmental variables can also affect the distribution and community structure of aquatic plants, the most relevant being water temperature light availability, and salinity [53][81]. Most aquatic macrophytes and some microalgae are able to tolerate conditions unfavorable for their growth and survival (e.g., suboptimal water temperatures, changes in water chemistry, low water levels, etc.) for limited periods.

The aquatic plant communities at the Cerro Bosta and Quebrada del Helecho localities were dominated by the rooted marsileaceous fern Regnellidium thomas-taylorii, which was associated with the presumably free-floating salviniaceous fern Paleoazolla and abundant microalgae (see reconstructions in Figs. 8, 9). These localities probably represented a sector of a lagoon system with eutrophic conditions and shallow water. At the Cañadón del Irupé locality (see reconstruction in Fig. 9), aquatic macrophytes were more diverse, with free-floating components represented by an araceous plant and water ferns in the family Salviniaceae (Azolla and Paleoazolla). There were also free-floating microphytes and rooted macrophytes, the latter including marsileaceous ferns and the eudicot Nelumbo (Nelumbonaceae). Based on the life habits of the nearest living relatives of these plants, this paleocommunity also probably occupied shallow waters of the lagoon system. The substratum may have been more organic than at the other localities as indicated by the carbonaceous nature of the fossil remains. The aquatic plant horizon identified in different sections of the La Colonia Formation suggests that freshwater conditions prevailed at this stage throughout the whole region. Periodic influx of saline water from the nearby sea caused retreat of freshwater macro- and microphytes to more suitable habitats in the coastal plain water bodies. Finally, the lack of frost tolerance in modern analogues of some components of the La Colonia Formation flora (e.g., Azolla, lemnoids, Marsiliaceae) suggests that water temperatures were rarely, if ever, too cold.

thumbnail
Figure 8. Reconstruction of the plant communities at the Cerro Bosta locality.

Surrounding (close-allochthonous) terrestrial vegetation: (a) Arecaceae, (A) Euterpe (Plantsystematics.org- DOL11633) and (b) Dicksoniaceae, (B) Dicksonia sellowiana (Photo by R. Moran, Plantsystematics.org- DOL75000). Autochthonous vegetation: Emergent-rooted macrophyte (c) Regnellidium thomas-taylorii, (C) Regnellidium diphyllum (Photo by D.W. Stevenson, Plantsystematics.org- DOL25635) and free-floating macrophyte (d) Azolla and Paleoazolla, (D) Azolla (Photo by R. Moran, Plantsystematics.org- DOL6803).

https://doi.org/10.1371/journal.pone.0104749.g008

thumbnail
Figure 9. Reconstruction of the plant communities at Cañadón del Irupé/Quebrada del Helecho localities.

Surrounding (close-allochthonous) terrestrial vegetation: (a) Arecaceae, (A) Euterpe (Plantsystematics.org- DOL11633), (b) Dicksoniaceae, (B) Dicksonia sellowiana (Photo by R. Moran, Plantsystematics.org-DOL75000), and (c) Typha-like, (C) Typha latifolia (Photo by K.C. Nixon, Plantsystematics.org- DOL6212). Autochthonous vegetation: Emergent-rooted macrophytes: (d) Mirasolita-Lugiomarsiglia, (D) Marsilea scalaripes (Photo by D. Nickrent, PhytoImages.siu.edu-DOL69348) and (e) Nelumbo puertae and reproductive structures, (E) Nelumbo lutea (Photo by R. Moran, Plantsystematics.org- DOL42110); and freeifloating macrophytes: (f) Araceae, (F) Pistia lactucoides (Photo by K.C. Nixon, Plantsystematics.org- DOL4884) and (g) Azolla and Paleoazolla, (G) Azolla (Photo by R. Moran, Plantsystematics.org- DOL6803).

https://doi.org/10.1371/journal.pone.0104749.g009

Surrounding vegetation.

Terrestrial vegetation surrounding the lagoon system can be divided into two prinicipal types based on the participation of different plant groups, the “wetlands” (or parautochthonous elements) and the “nearby upper lowlands” (allochthonous elements).

1.Wetlands or parautochtonous (marshes).

This terrestrial component is represented in the microflora by arecaceous (palm) and typhaceous pollen and in the macroflora by herbaceous monocots (probably Typhaceae) and several dicot leaves of uncertain affinities [30] (Table 1; Fig. 10). Dicksoniaceous ferns, represented by macro- and microfossils (Fig. 10A, C), are an important terrestrial component of the vegetation. We interpret these plants as having occupied a belt of vegetation surrounding the water bodies where a shallow water table was present. They probably represent a region of open vegetation composed of an understory of dicot shrubs or small trees (represented by leaf macrofossils) and herbaceous monocots (Typhaceae), a tree-fern stratum (Dicksoniaceae), and an overstory of taller palm trees (Arecaceae) (Fig. 8).

thumbnail
Figure 10. Main representatives of the surrounding lowland vegetation.

A: Dicksoniaceous fertile and sterile fronds; MPEF-Pb 6457. C: Dicksoneaceous spore massulae; BAFCB meb 263. B: Typhaceous leaf; MPEF-Pb 5074. D: Arecipites type pollen grain; BAFCB meb 261. E: Podocarpaceous bisaccate pollen grain; BAFCB meb 262. F: Classopolis pollen grain; BAFCB meb 259. G: Typhaceous pollen grain; BAFCB meb 260. C, D, E (SEM); F, G (LM). Scales: A, B = 1 cm; C = 20 µm; D = 5 µm; E, F, G = 10 µm.

https://doi.org/10.1371/journal.pone.0104749.g010

Extant genera of Typhaceae (Typha and Sparganium) are aquatic macrophytes; they can be considered caulescent planemergent or emergent benthophytes [57] or submerged/floating-leaved/emergent macrophytes [2][82][83][84][85]. A few fragments of parallel-veined, putatively typhaceous leaves were reported from the Cañadón del Irupé locality [30] (Fig. 10B). However, the presence of typhaceous plants is mostly based on several pollen grains of Sparganiaceaepollenites, a type similar to pollen produced by Sparganium and Typha [86], found at the Cerro Bosta and Cañadón del Irupé localities (Fig. 10D). Typically, Typhaceae grow in freshwater lagoons and stagnant ponds, although they sometimes occur in brackish environments or in swamps. Modern Typhaceae produce enormous amounts of pollen, and pollen grains that land in the water eventually sink and settle on the substrate [87][88]. This suggests that Spargiaceaepollenites pollen should be abundant if plants were producing it in loco at the La Colonia Formation localities. Because Sparganiaceaepollenites pollen was found in very small amounts within the palynoflora, we infer that the typhaceous plants were not growing within the lagoon itself, but were part of the vegetation belt surrounding it.

Macrofossil Dicksoniaceae are extremely abundant at the Quebrada del Helecho locality and occur more sporadically at the Cañadón del Irupé locality [30]. At both localities, dicksoniaceous ferns are represented by dispersed spores and spore massulae as well as fertile and sterile tripinnate pinnules (Fig. 10A). While the sporophytic phase is completely terrestrial in Dicksoniaceae, these ferns favor humid environments and are frequently found on riverbanks [89][90].

Palm (Arecaceae) pollen grains were found in sediments of the study localities as isolated grains (Fig. 10G) as well as clumps. When palm pollen is found in clumps, it is indicative that the pollen grains fell close to the source [91]. Although few species of palms grow in wetlands, several are characteristic of this type of environment. These include Manicaria saccifera, Mauritia flexuosa, and Euterpe oleracea, which today are quite common in the Orinoco Delta region of Venezuela [92], where they occur in coastal freshwater swamps, frequently in large, dense stands [92].

2. Nearby upper lowlands (allochtonous).

The plant remains of the nearby upper lowland flora are considered to be allochthonous. They are composed of plants that did not require direct contact with a permanent water body and were adapted to a deeper water table. The upper lowlands were inhabited primarily by conifers, in particular those yielding Classopollis (Cheirolepidiaceae) and podocarpaceous pollen (Fig. 10F). Some angiosperms were also present, as suggested by their dispersed pollen grains and undetermined leaf remains. Pollen include three species of Nothofagaceae, several types of Proteaceae, and several other unidentified eudicots and monocots.

Plant consumers: the associated fauna and its environmental and paleoecological implications

Undoubtedly, one of the best-preserved and most diverse latest Cretaceous meso- and macrofaunas in the Southern Hemisphere occurs in the La Colonia Formation. The fauna is composed of dinosaurs including carnosaurs, hadrosaurs, and ankylosaurs [93][94], aquatic and terrestrial turtles [95], plesiosaurs [96][97], snakes [98], birds [99], and mammals [34][100][101]. The presence of polycotylid plesiosaurs [96][97] and elasmosaurs [96] in the Cerro Bosta paleofauna supports the interpretation of lagoonal settings for these sections of the formation. Polycotylid plesiosaurs [102] are part of a derived clade of small plesiosaurs (actually pliosaurs) that lived in lagoonal, estuarine and freshwater environments [103]. Elasmosaurs are another group of small plesiosaurs that lived in the same types of paleoenvironments.

Of particular interest among the dinosaurs are several forms with aquatic or semi-aquatic lifestyles, the hadrosaurs and ankylosaurs. Hadrosaurs are traditionally considered important consumers of aquatic plants based on their ecology and adaptations, while ankylosaurs could have been consumers of aquatic plants as suggested from their dentition features adapted mainly for soft vegetation [104]. Turtles are the most abundant and diverse of the reptilian fauna in the La Colonia Formation, and some of them are also potential consumers of the aquatic vegetation. Plants such as green microalgae colonies, Azolla (a fast-growing fern that is also a good source of nitrogen due to its symbiosis with a cyanobacterium), and aquatic angiosperms could have served as nutritious sources of food for these herbaceous vertebrates [65].

True water bugs (Heteroptera, Nepomorpha) are also commonly found in the La Colonia Formation (Petrulevicius, pers. comm.). Nepomorphs are distributed worldwide, but are most diverse in the tropics, inhabiting different habitats ranging from shores of small ponds and banks of streams to deep inland water bodies [105]. These insects are characterized by their seasonal adaptations that permit them to survive under periodically fluctuating environmental conditions such as those that affect lagoon systems, especially during episodes when the lagoon is flooded with seawater [105]. In the La Colonia Formation, these water bugs most likely found shelter and food sources in the aquatic vegetation, probably creating their own microhabitats.

Biogeographic patterns

Most diverse modern aquatic plant communities are primarily distributed in the zone between the 30°N and 30°S latitude [1]. Species diversity is highest in the Neotropics, followed by the Afrotropics, Indo-Malaya, the Nearctic, and then the remaining phytogeographic regions [1]. The broad distribution of aquatic plants has classically been attributed to long-distance dispersal, in particular via migratory birds [2]. Continental drift and land connections also likely played a major role, and were probably particularly important before the evolution of birds in the Cretaceous [24].

This tendency toward broad geographic distribution was established early in the history of aquatic macrophytes. Fossil Nelumbonaceae, for example, are mainly recorded from Laurassia with a curious record in southern South America at the La Colonia and Lefipán formations by the Late Cretaceous [29][30], initially explained by the spreading of the group [106][107], but suggesting continuous and similar conditions in fresh water coastal ecosystems from North to South America that would have allowed some migratory flow. Interestingly, the record of the family in South America is only represented by fossil forms from Patagonia [29][30], with the current presence of Nelumbo on Gondwanan continents (northern South America and northern Australia) probably being attributable to human-mediated dispersal [107]. Additionally, aquatic marsileaceous ferns are found throughout the world in the Cretaceous, with an extensive and cosmopolitan dispersed spore record that extends throughout the period (for summaries of many known occurrences, see Arcellites, Molaspora, Rodeites and Crybelosporites in [9][108]). Aquatic Araceae are clearly present by the latest Cretaceous to Paleocene not only in South America, but also in North America and northeastern Asia [23][27][70][109][110]

Latest Cretaceous and Paleocene aquatic communities reported from the Northern Hemisphere include those from Europe (19), Mongolia/NE Asia [22][24], and western North America [24][27], which share elements with the La Colonia flora. Although the taxonomic composition of these floras is not the same at generic level, commonalities are observed at the level of plant family or plant habit. All are composed of floating microalgae (e.g., Pediastrum), floating aquatic ferns (e.g., Azolla), rooted salvinialean ferns (e.g., Hydropteris, Marsileaceae), and floating and/or rooted angiosperms (Araceae and Nelumbonaceae), with a terrestrial component consisting of selected coniferous gymnosperms, ferns, and angiosperms. The presence of aquatic swamp communities dominated by Azolla and Pediastrum was reported from the latest Cretaceous Hell Creek Formation [24], North Dakota, USA, in paleoenvironments analogous to modern subtropical swamps. These elements are abundant in the Cañadón del Irupé/Quebrada del Helecho localities, suggesting a similar environment.

In Patagonia, an aquatic plant community has been detected from the nearbyPaleocene (Danian)-aged Bororó Formation [111]. This community comprises some of the elements found within the La Colonia flora, including Chlorococcales, Zygnematales, Salviniaceae, pteridophytes, and conifers, as well as angiosperms in the families Araceae, Arecaceae, Typhaceae, and Proteaceae. The occurrence of the same taxa suggests that the Cretaceous/Paleogene boundary event did not affect aquatic plant communities, which retained approximately similar structure and composition during the transition between the Maastrichtian and the basalmost Paleocene (Danian). Similar conclusions were obtained from the coeval and nearby Lefipán Formation in northwestern Patagonia [112].

Concluding remarks

Near the end of the Cretaceous in central and southern Patagonia, marine transgressions favored oceanic climates that influenced the composition of the vegetation. In general, the Patagonian paleofloras were dominated by angiosperms, suggesting warm-temperate to subtropical humid conditions for the region [113]. Notably, the frost intolerance of the modern analogues of some plants found in the La Colonia Formation sediments—such as Salviniaceae, Marsiliaceae, Araceae, and palms—also indicates a tropical to subtropical climate.

Based on the data presented in this report, it is clear that by the latest Cretaceous there was a suite of lagoonal coastal wetlands in Patagonia, that promoted the proliferation and, later, the preservation of freshwater plant communities. The interpretation of the paleofloral localities as representing a lagoonal environment is confirmed by the presence and types of free-floating aquatic macrophytes (pleustophytes), free-floating microphytes, and rooted macrophytes with floating or emergent leaves (planmergent or emergent benthophytes) that constituted the main components of the aquatic vegetation. A small number of terrestrial and aquatic macrophytes surrounded the water bodies, and additional allochthonous components occurred on higher ground. The clastic conditions of these southern hemisphere mid-latitude environments did not allow for the development of mangrove vegetation that is normally linked to more calcareous shallow platforms.

Many of the records reported for the La Colonia paleocommunity are of significant value. The Salviniaceae, Nelumbonaceae, and Araceae constitute the first macrofossil records for these Southern Hemisphere families. The occurrence of arecaceaous pollen within a wetland environment suggests that palms were found in both drier and in wetland areas in Patagonia in the past. In summary, the La Colonia paleocommunity is the first aquatic plant community described for the Southern Hemisphere based on both macro- and microfossils, and, thus, provides a unique window into better understanding the Late Cretaceous Patagonia and Southern Hemisphere.

Acknowledgments

We would like to thank the Museo Paleontológico Egidio Feruglio (MEF) and E. Ruigómez and other MEF staff for access to the fossil plant collections and assistance in specimen preparation. Ignacio Escapa provided invaluable help with illustrations. Florencia Gigena prepared illustrations in fine detail. Field crew members who helped in collecting the fossil specimens include: M. Caffa, J. Carballido, M. Dellocca, C. González, and P. Puerta. We particularly thank Drs. Zulma Gasparini (La Plata Museum), Ana Parras (Univ. Nac. La Pampa), Julio Varela (Univ. Nac. La Pampa) and, Gerardo Cladera (MEF) provided invaluable sedimentological information used for drawing sections depicted in Fig. 2. J. Svitko (Cornell University) assisted with assembling the bibliography and plates. R. Moran and D.W. Stevenson (New York Botanical Garden), D. Nickrent (Southern Illinois University, PhytoImages.siu.edu), and K.C. Nixon (Cornell University, Plantsystematics.org) allowed use of images of extant taxa. The use of MEB facilities at the Museo Argentino Ciencias Naturales B. Rivadavia is particularly appreciated. David W. Taylor, an anonymous reviewer, and PlosOne Academic Editor Dana Royer provided insightful comments that greatly improved the manuscript.

Author Contributions

Conceived and designed the experiments: NRC MAG MCZ EJH. Performed the experiments: NRC MAG MCZ EJH. Analyzed the data: NRC MAG MCZ EJH. Contributed reagents/materials/analysis tools: NRC MAG MCZ EJH. Contributed to the writing of the manuscript: NRC MAG MCZ EJH.

References

  1. 1. Cook CDK (1996) Aquatic Plant Book. SPB Academic Publishing, Amsterdam 228 pp.
  2. 2. Chambers PA, Lacoul P, Murphy KJ, Thomaz SM (2008) Global diversity of aquatic macrophytes in freshwater. Hydrobiol 595: 9–26.
  3. 3. Sculthorpe CD (1967) The biology of aquatic vascular plants. Edward and Arnold Publishers, London.
  4. 4. Lacoul P, Freedman B (2006) Environmental influences on aquatic plants in freshwater ecosystems. Environ Rev 14: 89–136.
  5. 5. Collinson ME (1988) Freshwater macrophytes in palaeolimnology. Palaeog Palaeoclim Palaeoecol 62: 317–342.
  6. 6. Janauer GA (2001) Is what has been measured of any direct relevance to the success of the macrophyte in its particular environment? J of Limnol 60(Suppl. 1): 33–38.
  7. 7. Rothwell GW, Stockey RA (1994) The role of Hydropteris pinnata gen. et sp. nov. in reconstructing the cladistics of heterosporous ferns. Am J Bot 81: 479–492.
  8. 8. Martin-Closas C (2003) The fossil record and evolution of fresh water plants: a review. Geol Acta 1: 315–338.
  9. 9. Kovach WL, Batten DJ (1989) Worldwide stratigraphic occurrences of Mesozoic and Tertiary megaspores. Palyn 13: 247–277.
  10. 10. Lupia R, Schneider H, Moeser GM, Pryer KM, Crane PR (2000) Marsileaceae sporocarps and spores from the Late Cretaceous of Georgia, U.S.A. Int J Plant Sci 161: 975–988.
  11. 11. Yamada T, Kato M (2002) Regnellites nagashimae gen. et sp. nov., the oldest macrofossil of Marsileaceae, from the Upper Jurassic to Lower Cretaceous of western Japan. Int J Plant Sci 163: 715–723.
  12. 12. Blanc-Louvel C (1984) Le genre “Ranunculus L.” dans le Berriasien (Crétacé inf.) de la province de Lérida (Espagne). Ilerda 45: 83–92.
  13. 13. Wang X, Zheng XT (2012) Reconsiderations on two characters of early angiosperm Archaeofructus. Paleoworld 21: 193–201.
  14. 14. Wang H, Dilcher DL (2006) Aquatic angiosperms from the Dakota Formation (Albian, Lower Cretaceous), Hoisington III locality, Kansas, USA. Int J Plant Sci 167: 385–401.
  15. 15. Gandolfo MA, Nixon K, Crepet W (2004) Cretaceous flowers of Nymphaeaceae and implicatioins for complex insect entrapment pollination mechanismms in early Angiospers. Proc Nat Acad Sci U S A 101: 8056–8060.
  16. 16. Coiffard C, Mohr B, Bernardez de Oliveira M (2013) Jaguariba wiersemana gen. nov. Et sp. nov., an Early Cretaceous member of crown group Nyumphaeales (Nymphaeaeae) fromnorthern Gondwana. Taxon 62: 141–151.
  17. 17. Mohr B, Friis EM (2000) Early angiosperms from the Aptian Crato Formation (Brazil), a preliminary report. Int J Plant Sci 161: 155–167.
  18. 18. Batten DJ, Lister JK (1988) Early Cretaceous dinoflagellate cysts and chlorococcalean algae from freshwater and low salinity palynofacies in the English Wealden. Cret Res 9: 337–367.
  19. 19. Batten DJ (1996) Colonial Chlorococcales. In: Palynology: Principles and Applications, Am Assoc Strat Palyn Found Jansonius J, McGregor DC, editors. 1: 191–203.
  20. 20. Zippi PA (1998) Freshwater algae from the Mattagami Formation (Albian), Ontario: Paleoecology, botanical affinities, and systematic taxonomy. Micropaleont 44(Suppl.1): 1–78.
  21. 21. Mai DH (1985) Entwicklung der Wasser und Sumpfpflanzen-Gesellschaften Europas von der Kreide bis ins Quartär. Flora 176: 449–511.
  22. 22. Krassilov V, Makulbekov NM (1995) Maastrichtian aquatic plants from Mongolia. Paleontol J 29: 119–140.
  23. 23. Stockey RA, Rothwell GW, Johnson KR (2007) Cobbania corrugata gen. et comb. nov. (Araceae): a floating aquatic monocot from the Upper Cretaceous of western North America. Am J Bot 94: 609–624.
  24. 24. Krassilov V, Kodrul T, Maslova N (2009) Plant systematics and differentiation of species over trans-Beringian land connections including a newly recognized cupressaceous conifer Ditaxocladus Guo & Sun. Bull. Geosci 85: 95–110.
  25. 25. Golovneva LB (2000) Aquatic plant communities at the Cretaceous-Paleogene boundary in north-eastern Russia. Acta Paleobot 40: 139–151.
  26. 26. Vajda V, Lyson T, Bercovici A, Doman J, Pearson D (2013) A snapshot into the terrestrial ecosystem of and exceptionally well-preserved dinosaur (Hadrosauridae) from the Upper Cretaceous of North Dakota, USA. Cret Res 46: 114–122.
  27. 27. McIver EE, Basinger JF (1993) Flora of the Ravenscrag Formation (Paleocene), Southwestern Saskatchewan, Canada. Palaeontogr Can 10: 101.
  28. 28. Archangelsky A, Phipps CJ, Taylor TT, Taylor EL (1999) Paleoazolla, a new heterosporous fern from the Upper Cretaceous of Argentina. Am J Bot 86: 1200–1206.
  29. 29. Gandolfo MA, Cúneo NR (2005) Fossil Nelumbonaceae from the La Colonia Formation (Maastrichtian, Upper Cretaceous), Chubut, Patagonia, Argentina. Rev Palaeobot Palyn 133: 169–178.
  30. 30. Gandolfo MA, Cúneo NR, Hermsen EJ (2014) Reporte preliminar sobre la paleoflora de la Formación La Colonia (Campaniano-Maastrichtiano, Cretácico tardío), Chubut, Patagonia, Argentina. Rev Asoc Geol Mex 66: 11–23.
  31. 31. Gallego J, Gandolfo MA, Cúneo NR (In press) Fossil Araceae from the Late Cretaceous of Patagonia, Argentina, with implications on the origin of free-floating aquatic aroids. Rev Palaeobot Palyn
  32. 32. Cúneo NR, Hermsen EJ, Gandolfo MA (2013) Regnellidium (Salviniales, Marsileaceae) macrofossils and associated spores from the Late Cretaceous of South America. Int J Plant Sci 174: 340–349.
  33. 33. Hermsen EJ, Gandolfo MA, Cúneo NR (2013) New Marsileaceous Fossils from the Late Cretaceous of South America and a Reevaluation of Marsileaceaephyllum. Plant Syst Evol DOI:https://doi.org/ 10.1007/s00606-013-0886-7.
  34. 34. Pascual R, González P, Ardolino A, Puerta PF (2000) A highly derived docodont from the Patagonian Late Cretaceous: evolutionary implications for Gondwanan mammals. Geodivers 22: 395–414.
  35. 35. Náñez C, Malumián N (2008) Paleobiogeografía y paleogeografía del Maastrichtiense marino de la Patagonia, Tierra del Fuego y la Plataforma Continental Argentina, según sus foraminíferos bentónicos. Rev Esp Paleont 23: 273–300.
  36. 36. Guler MV, Borel CM, Brinkhuis H, Navarro E, Astini R (2014) Brackish to freshwater dinoflagellate cyst assemblages from the La Colonia Formation (Paleocene?), Northeastern Patagonia, Argentina. Ameghiniana 51: 141–153.
  37. 37. Ardolino A, Delpino D (1987) Senoniano (continental-marino). Comarca Norpatagónica, Provincia del Chubut, Argentina. X Cong Geol Arg, Tucumán, Actas 3: 193–196.
  38. 38. Malumián N, Náñez C (2011) The Late Cretaceous–Cenozoic transgressions in Patagonia and the Fuegian Andes: foraminifera, palaeoecology, and palaeogeography. Biol J Linn Soc 103: 269–288.
  39. 39. Franzese J, Spalletti L, Gómez Pérez I, MacDonald D (2003) Tectonic and paleoenvironmental evolution of Mesozoic sedimentary basins along the Andean foothills of Argentina. J South Am Earth Sci 16: 81–90.
  40. 40. Scasso RA, Aberhan M, Ruiz L, Weidemeyer S, Medina FA, et al. (2012) Integrated bio- and lithofacies analysis of coarse-grained, tide-dominated deltaic environments across the Cretaceous/Paleogene boundary in Patagonia, Argentina. Cret Res 36: 37–57.
  41. 41. Ardolino AA, Franchi M (1996) Hoja geológica 4366–I Telsen. Provincia del Chubut. Dirección Nacional del Servicio Geológico, Bol 215, Buenos Aires.
  42. 42. Gandolfo MA, Zamaloa MC, Cúneo NR, Archangelsky A (2009) Fossil Potamogetonaceae from the Paleogene of Chubut, Patagonia, Argentina. Int J Plant Sci 170: 419–428.
  43. 43. O'Gorman JP, Salgado L, Varela J, Parras A (2013) Elasmosaurs (Sauropterygia, Plesiosauria) from the La Colonia Formation (Campanian-Maastrichtian), Argentina. Alcheringa 37: 259–267.
  44. 44. Stamp LD (1921) On cycles of sedimentation in the Eocene strata of the Anglo-Franco-Belgian basin. Geol Mag 58: 108–114.
  45. 45. Swift DJP (1968) Coastal erosion and transgressive stratigraphy. J Geol 76: 444–456.
  46. 46. Dalrymple RW, Zaitlin BA, Boyd R (1992) Estuarine facies models: conceptual basis and stratigraphic implications. J Sed Petrol 62: 1130–1146.
  47. 47. Boyd R, Dalrymple R, Zaitlin BA (1992) Classification of clastic coastal depositional environments. Sed Geol 80: 139–150.
  48. 48. Reading HG, Collinson JD (1996) Clastic Coasts. In: Readin, H.G (Ed.), Sedimentary Environments, processes, facies and stratigraphy. Blackwell, Malden, Massachusetts, 154–231.
  49. 49. Reinson GE (1992) Transgressive barrier island and estuarine systems. In: Walker, R.G., James, N.P. (Eds.), Facies Models: response to sea level change. St. John's, Newfoundland, 179–194.
  50. 50. Sanders JE, Kumar N (1975) Evidence of shoreface retreat and in-place “drowning” during Holocene submergence of barriers, shelf off Fire Island, New York. Geol Soc Am Bull 86: 65–76.
  51. 51. Cattaneo A, Steel RJ (2002) Transgressive deposits: a review of their variability. Earth Sci Rev 62: 187–228.
  52. 52. Hayes MO (1979) Barrier-island morphology as a function of tidal and wave regime. In: Lestherman, SP, ed., Barrier Islands-from the Gulf of St. Lawrence to the Gulf of Mexico. New York, Academic Press, 1–28.
  53. 53. Koch E (2001) Beyond light: physical, geological and geochemical parameters as possible submersed aquatic vegetation habitat requirements. Estuaries 24: 1–17.
  54. 54. Crow GE (1993) Species diversity in aquatic angiosperms: latitudinal patterns. Aq Bot 44: 229–258.
  55. 55. Horne JC, Ferm JC, Caruccio FT, Baganz BP (1978) Depositional models in coal exploration and mine planning in Appalachian region:. Am Assoc Petrol Geol Bull 62: 2379–2411.
  56. 56. Schuyler AE (1984) Classification of life forms and growth forms of aquatic macrophytes. Bartonia 50: 8–11.
  57. 57. Scremin-Dias E (2003) Tropical aquatic plants: morphoanatomical adaptations. In: Tropical biology and conservation management, Vol. 1. Encyclopedia of life support systems.
  58. 58. Batten DJ, Grenfell HR (1996) Chapter 7D. Botryococcus. In: Palynology: Principles and Applications, Am Assoc Strat Palyn Found Jansonius J, McGregor DC, editors. 1: 205–214.
  59. 59. Zamaloa MC, Tell G (2005) The fossil record of freshwater micro-algae Pediastrum Meyen (Chlorophyceae) in southern South America. J Paleolim 34: 433–444.
  60. 60. Van Geel B, Grenfell HR (1996) Chapter 7A. Spores of Zygnemataceae. In: Palynology: principles and applications. Am Assoc Strat Palyn Found Jansonius J, McGregor DC, editors. 1: 173–179.
  61. 61. Zamaloa MC (1996) Asociación de zigósporas de Zygnemataceae (Chlorophyta) en el Terciario Medio de Tierra del Fuego, Argentina. Ameghiniana 33: 179–184.
  62. 62. Tryon R, Tryon AF (1982) Ferns and allied plants. Springer-Verlag, New York.
  63. 63. Schneller JJ (1990) Azollaceae. In: Kramer, K.U., Green, P.S. (Eds.), The Families and Genera of Vascular Plants. I Pteridophytes and Gymnosperms, New York, p. 57–60.
  64. 64. Saunders RMK, Fowler K (1993) The supraspecific taxonomy and evolution of the fern genus Azolla (Azollaceae). Plant Syst Evol 184: 175–193.
  65. 65. Metzgar JS, Schneider H, Pryer KM (2007) Phylogeny and divergence time estimates for the fern genus Azolla (Salviniaceae). Int J Plant Sci 168: 1045–1053.
  66. 66. Heusser CJ, Denton GH, Hauser A, Anderson BG, Lowell TV (1996) Water Fern (Azolla filiculoides Lam.) in Southern Chile as an index of paleoenvironment during Early Deglaciation. Artic Alpine Res 28: 148–155.
  67. 67. Rai V, Rai AK (1999) Growth behaviour of Azolla pinnata at various salinity levels and induction of high salt tolerance. Plant and Soil 206: 79–84.
  68. 68. Rai V, Rai AK (2000) Response of NaCL-adapted and unadapted Azolla pinnata-Anabaena azollae complex to salt stress: partial photosynthetic process and respiration. Symbiosis 29: 246–261.
  69. 69. Tryon AF, Lugardon B (1991) Spores of the Pteridophyta. Springer-Verlag, New York.
  70. 70. Stockey RA, Hoffman GL, Rothwell GW (1997) The fossil monocot Limnobiophyllum scutatum: resolving the phylogeny of Lemnaceae. Am J Bot 84: 35–368.
  71. 71. Cook CDK (1990) Aquatic Plant Book. SPB Academic Publishing, The Hague. The Netherlands.
  72. 72. Schultz AR (1949) Contribuicoes ao conhecimiento de Regnellidium diphyllum Lindm. Lilloa 17: 139–144.
  73. 73. Schneider H, Pryer KM (2002) Structure and function of spores in the aquatic heterosporous fern family Marsileaceae. Int J Plant Sci 163: 485–505.
  74. 74. Alonso Paz E, Bassagoda MJ (2002) Revisión de las Marsileaceae del Uruguay y primera cita de Pilularia americana A. Braun. Museos Nacionales de Historia Natural y Antropología, Com Bot 125: 1–8.
  75. 75. Kramer KU (1990) Marsileaceae. In: Kramer K.U., Green, P. S., (Eds.). Pteridophytes and Gymnosperms. Vol. 1- The families and genera of vascular plants. Kubitzki, K., (Ed), Springer Verlag, Berlin, 180–183.
  76. 76. Johnson DM (1986) Systematics of the New World species of Marsilea (Marsileaceae). Syst Bot Monog 11: 1–87.
  77. 77. Gupta KM (1962) Marsilea. Botanical Monographs, Vol. 2, 1–113. Council of Scientific and Industrial Research, New Delhi.
  78. 78. Collinson ME (2001) Diversification of modern heterosporous pteridophytes. In: Pollen and Spores, Syst Assoc Spec Blackmore S, Barnes SH, editors. Vol 44: 119–150.
  79. 79. Williamson PS, Schneider EL (1993) Nelumbonaceae. In: K. Kubitzki (Ed.), The families and genera of vascular plants, Springer-Verlag, Berlin, 470–473.
  80. 80. Thomaz SM, Esteves FA, Murphy KJ, dos Santos AM, Caliman A, et al.. (2008) Aquatic macrophytes in the tropics: ecology of populations and communities, impacts of invasions and human use. In: Tropical biology and conservation management, Vol. 4. Encyclopedia of Life Support Systems.
  81. 81. Flynn KM, McKee KL, Mendelssohn IA (1995) Recovery of freshwater marsh vegetation after a saltwater intrusion event. Oecologia 103: 63–72.
  82. 82. Finlayson M, Forrester RI, Mitchell DS, Chick AJ (1985) Identification of native Typha species in Australia. Aust J Bot 33: 101–107.
  83. 83. Grace JB, Harrison JS (1986) The biology of Canadian weeds. 73. Typha latifolia L., Typha angustifolia L. and Typha glauca Godr. Can J Plant Sci 66: 361–379.
  84. 84. Thieret JW (1982) The Sparganiaceae in the southeastern United States. J Arnold Arboret 63: 341–355.
  85. 85. Thieret JW, Luken JO (1996) The Typhaceae in the southeastern United States. Harv Pap in Bot 8: 27–56.
  86. 86. Punt W (1975) Northwest European Pollen Flora, 5. Sparganiaceae and Typhaceae: Rev Palaeobot Palyn 19: 75–88.
  87. 87. Cambon G, Suc JP, Aloisi JC, Giresse P, Monaco A, et al. (1997) Modern pollen deposition in the Rhone delta area (lagoonal and marine sediments), France. Grana 36: 105–113.
  88. 88. Mahabalé TS (1968) Spores and pollen grains of water plants and their dispersal. Rev Palaeobot Palyn 7: 285–296.
  89. 89. Kramer KU (1990) Dicksoniaceae. In: Kramer K.U., Green, P. S., (Eds.). Pteridophytes and Gymnosperms. Vol. 1- The families and genera of vascular plants. Kubitzki, K., (Ed), Springer Verlag, Berlin, 94–99.
  90. 90. Fiori CCL, Santos M, Randi AM (2009) Aspects of gametophyte development of Dicksonia sellowiana Hook (Dicksoniaceae): and endangered tree fern indigenous to South and Central America. Am Fern J 99: 207–216.
  91. 91. Hoffman CC (2002) Pollen distribution in sub-Recent sedimentary environments of the Orinoco Delta (Venezuela), an actuo-paleobotanical study. Rev Paleobot Palyn 119: 191–217.
  92. 92. Dransfield J, Uhl NW, Asmussen CB, Baker WJ, Harley MM, et al.. (2008) Genera Palmarum- The Evolution and Classification of Palms. Kew Publishing, Royal Botanic Gardens, Kew.
  93. 93. Bonaparte JF (1985) A horned Cretaceous carnosaur from Patagonia. Nat Geog Res 1: 263–272.
  94. 94. Gasparini Z, De la Fuente M (2000) Tortugas y plesiosauros de la Formación La Colonia (Cretácico superior) de Patagonia, Argentina. Rev Esp Paleont 15: 23–35.
  95. 95. Sterli J, de la Fuente MS (2011) A new turtle from La Colonia Formation (Campanian-Maastricthian), Patagonia, Argentina, with remarks on the evolution of the vertebral column in turtles. Palaeont 54: 63–78.
  96. 96. Gasparini Z, Casadio S, Fernández M, Salgado L (2001) Marine reptiles from the Late Cretaceous of northern Patagonia. J South African Earth Sci 14: 51–60.
  97. 97. O'Gorman JP, Gasparini Z (2013) Revision of Sulcusuchus erraini (Sauropterygia, Polycotylidae) from the Upper Cretaceous of Patagonia, Argentina. Alcheringa 37: 163–176.
  98. 98. Albino AM (2000) New record of snakes from the Cretaceous of Patagonia (Argentina). Geodiversitas 22: 247–253.
  99. 99. Lawver DR, Debee AM, Clarke JA, Rougier GW (2011) A New Enantiornithine bird from the Upper Cretaceous La Colonia Formation of Patagonia, Argentina. Ann Carn Mus 80: 35–42.
  100. 100. Kielan–Jaworowska Z, Ortiz–Jaureguizar E, Vieytes C, Pascual R, Goin FJ (2007) First ?cimolodontan multituberculate mammal from South America. Acta Palaeont Pol 52: 257–262.
  101. 101. Rougier GW, Forasiepi AM, Hill RV, Novacek MJ (2009) New mammalian remains from the Late Cretaceous La Colonia Formation, Patagonia, Argentina. Acta Palaeont Pol 54: 195–212.
  102. 102. Benson RBJ, Evans M, Druckenmiller PS (2012) High diversity, low disparity and small body seize in pleiosaurs (Reptilia, Sauropterrygia) from the Triassic-Jurassic boundary. PLoS ONE 7: e31838.
  103. 103. Cruickshank A (1997) A lower Cretaceous pliosaurid from South Africa. Ann S Afr Mus 105: 207–226.
  104. 104. Dettman M, Molnar RE, Douglas JG, Burger D, Fielding C, et al. (1992) Australian Cretaceous terrestrial faunas and floras: biostratigraphic and biogeographic implications. Cret Res 13: 207–262.
  105. 105. Saulich AH, Musolin DL (2007) Seasonal development of aquatic and semiaquatic true bugs (Heteroptera). St. Peterburg University Press, Saint Peterburg.
  106. 106. Estrada-Ruiz E, Upchurch GR Jr, Wolfe JA, Cevallos-Ferriz SRS (2011) Comparative morphology of fossil and extant leaves of Nelumbonaceae, including a new genus from the Late Cretaceous of western North America. Syst Bot 36: 337–351.
  107. 107. Li Y, Smith T, Svetlana P, Yang J, Jin JH, Li CS (2014) Paleobiogeography of the lotus plant (Nelumbonaceae: Nelumbo) and its bearing on the paleoclimatic changes. Palaeogeog Palaeoclim Palaeoecol 399: 284–293
  108. 108. Batten DJ, Kovach WL (1990) Catalog of Mesozoic and Tertiary megaspores. Am Assoc Strat Palyn Cont Ser 24.
  109. 109. Kvaček Z (1995) Limnobiophyllum Krassilov - a fossil link between the Araceae and the Lemnaceae. Aq Bot 50: 49–61.
  110. 110. Kvaček J, Herman AB (2004) Monocotyledons from the Early Campanian (Cretaceous) of Grunbach, Lower Austria. Rev Palaeobot Palyn 128: 323–353.
  111. 111. Volkheimer W, Scafati L, Melendi DL (2007) Palynology of a Danian warm climatic wetland in Central Northern Patagonia, Argentina. Rev Esp Micropaleont 39: 117–134.
  112. 112. Barreda VD, Cúneo NR, Wilf P, Currano ED, Scasso RA, et al. (2012) Cretaceous/Paleogene floral turnover in Patagonia: drop in diversity, low extinction, and a Classopollis spike. PLoS ONE 7: e52455.
  113. 113. Iglesias A, Artabe AE, Morel EM (2011) The evolution of Patagonian climate and vegetation from the Mesozoic to the present. Biol J Linn Soc 103: 409–22.