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Can the Life-History Strategy Explain the Success of the Exotic Trees Ailanthus altissima and Robinia pseudoacacia in Iberian Floodplain Forests?

  • Pilar Castro-Díez ,

    mpilar.castro@uah.es

    Affiliation Department of Life Sciences (U.D. Ecology), University of Alcalá, Alcalá de Henares, Madrid, Spain

  • Guillermo Valle,

    Affiliation Department of Life Sciences (U.D. Ecology), University of Alcalá, Alcalá de Henares, Madrid, Spain

  • Noelia González-Muñoz,

    Affiliations Department of Life Sciences (U.D. Ecology), University of Alcalá, Alcalá de Henares, Madrid, Spain, Laboratory of Systematic Ecology and Evolution, University of Paris-Sud, Paris, France

  • Álvaro Alonso

    Affiliation Department of Life Sciences (U.D. Ecology), University of Alcalá, Alcalá de Henares, Madrid, Spain

Can the Life-History Strategy Explain the Success of the Exotic Trees Ailanthus altissima and Robinia pseudoacacia in Iberian Floodplain Forests?

  • Pilar Castro-Díez, 
  • Guillermo Valle, 
  • Noelia González-Muñoz, 
  • Álvaro Alonso
PLOS
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Abstract

Ailanthus altissima and Robina pseudoacacia are two successful invasive species of floodplains in central Spain. We aim to explain their success as invaders in this habitat by exploring their phenological pattern, vegetative and sexual reproductive growth, and allometric relations, comparing them with those of the dominant native tree Populus alba. During a full annual cycle we follow the timing of vegetative growth, flowering, fruit set, leaf abscission and fruit dispersal. Growth was assessed by harvesting two-year old branches at the peaks of vegetative, flower and fruit production and expressing the mass of current-year leaves, stems, inflorescences and infrutescences per unit of previous-year stem mass. Secondary growth was assessed as the increment of trunk basal area per previous-year basal area. A. altissima and R. pseudoacacia showed reproductive traits (late flowering phenology, insect pollination, late and long fruit set period, larger seeds) different from P. alba and other native trees, which may help them to occupy an empty reproductive niche and benefit from a reduced competition for the resources required by reproductive growth. The larger seeds of the invaders may make them less dependent on gaps for seedling establishment. If so, these invaders may benefit from the reduced gap formation rate of flood-regulated rivers of the study region. The two invasive species showed higher gross production than the native, due to the higher size of pre-existing stems rather than to a faster relative growth rate. The latter was only higher in A. altissima for stems, and in R. pseudoacacia for reproductive organs. A. altissima and R. pseudoacacia showed the lowest and highest reproductive/vegetative mass ratio, respectively. Therefore, A. altissima may outcompete native P. alba trees thanks to a high potential to overtop coexisting plants whereas R. pseudoacacia may do so by means of a higher investment in sexual reproduction.

Introduction

In the last decades, the scientific interest in explaining causes and consequences of biological invasions has drastically increased [1][4], partly due to the social and economic impact of some invasion events [5], [6]. In the case of plants, many studies have identified functional traits associated to the invasive success, such as high growth rate, low cost of tissue production, resprouting ability, small seed size, profuse seed production, long flowering period or N-fixation ability [7][10]. In addition, different properties of ecosystems promoting invasions have been reported, such as high and fluctuating resource availability, high frequency of disturbances, biogeographic isolation, existence of empty niches or high spatial/temporal heterogeneity [11][16]. However, most studies agree in the context-dependency of plant traits and of the ecosystem properties associated to invasions [10], [17], [18]. In particular, it has been suggested that the chances of success, as well as the magnitude of the impact, largely depends on the functional difference between the exotic species and the prevailing native vegetation [2], [19], [20].

Recent studies have emphasized the relevance of phenological differences between exotic and native plants as a mechanism favouring invasions [21][24]. For instance, a different phenology allows an exotic species to benefit from resources (radiation, pollinators, nutrients) that are temporally available [21], [25] and contributes to stabilize the interactions between the exotic and the native species [26][28]. In addition, a different phenology may also provide exotics certain competitive advantage, either because earlier phenology allows sequestering resources first [29], because a later phenology allows higher production after escaping from competition by early-senescent native plants [24], [25], [30][32], or because a more extended phenology provides greater access to resources [10], [33], [34] (see Wolkovich & Clealand, 2011 for a review). Other features recently recorded as relevant for the success of exotic plants as invaders are vegetative growth and sexual reproduction. A high vegetative growth rate has been frequently found in invasive species [8], [35]. This trait is largely responsible for the competitive capacity of a plant species [36]. High seed production has been also shown as a relevant contributor to invasiveness, as it allows an efficient spread [37], [38]. However, there is a trade-off between investment in vegetative growth and sexual reproduction [39], [40]. The balance between both plant functions is very dependent on the selective forces of the environment where the species have evolved. In this sense, classical r-K and CSR hypotheses predict that frequently-disturbed environments promote investment to sexual reproduction at the expense of growth, while stable environments, where competition is the main selective force, foster growth over sexual reproduction [36], [41], [42]. Therefore a sudden change in the disturbance regime may reduce the fitness of the native species and promote invasions by exotic plants with growth/reproduction balance more suited to the new conditions.

In the Mediterranean part of Europe, floodplains are one of the habitats more susceptible to exotic plant invasions [43]. This is partly because river floods bring about some of the properties of invasion-prone environments mentioned above, such as high and fluctuating resource availability, and spatial and temporal heterogeneity [44], [45]. Besides, rivers are efficient dispersal agents for exotic seeds [44], [46], [47]. Finally, human management of rivers and floodplains (e.g. flow regulation, removal of riparian forests, deepening of water tables) can alter selective forces in riparian forests at a rate that cannot be tracked by genetic changes in long-life plants, such as trees. Thus, these human actions may facilitate plant invasion by reducing native plant fitness under the new conditions and by providing conditions that directly benefit invading species [48], [49].

In Mediterranean riparian forests of central Spain, the native Populus alba L. dominates clay-rich basic soils of middle or low river stretches, where it coexists with other less abundant native trees, such as Salix alba L., Populus nigra L., Fraxinus angustifolia L. and Ulmus minor Mill. [50]. Since the 19th century these species also co-occur with naturalized exotic trees, such as Ailanthus altissima (Mill.) Swingle and Robinia pseudoacacia L., which are native to E-China and the Appalachian Mountains (USA), respectively [51][53]. These exotic trees are efficient colonizers of disturbed sites, but they also colonize riparian forest and displace natural vegetation [51][54]. Both species possess many of the functional traits conferring invasive success, such as N-fixation ability in R. pseudoacacia, profuse seed production and fast growth in A. altissima and vegetative reproduction by root suckering in both species [52][54]. Besides, the two species are well known for their ecological impacts on the invaded ecosystems. For instance, A. altissima may increase soil fertility [55], [56] and suppress native vegetation by competition and/or allelopathic effects [52], [57], [58]. R. pseudoacacia may increase the input of atmospheric N into the soil [59], [60], but the high lignin content of its leaves make their decomposition slower than that of co-occurring native deciduous leaves of riparian forests [61][63]. This may explain the low colonization of R. pseudoacacia leaves by aquatic invertebrates [63]. Accordingly, both species are included in the list of the top 100 more aggressive invaders in Europe [64]. However, little effort has been done so far to understand the context-specific factors explaining the success of these invasive species in P. alba-dominated riparian forests of Central Spain.

We aim to explain the invasive success of A. altissima and R. pseudoacacia in the above described community by comparing their life-history strategies with that of the dominating native tree in the recipient community, P. alba. We hypothesize that (1) the exotic species differ in phenology from the dominating natives [21]; (2) a different organ phenology allows the invaders to achieve larger relative production of that organ, due to earlier resource sequestration and/or longer resource uptake period; and (3) given that flood regulation has decreased the disturbance frequency of floodplains, successful invaders are expected to be pre-adapted to more stable environments by a higher vegetative to reproductive mass ratio than the native species.

Methods

Study Species and Site

This study was conducted in the floodplains of a medium-low stretch of the Henares River in central Spain (Province of Madrid), which is a public non protected area where no permit access is required. The study area spans over 22 km, from Alcalá de Henares to Mejorada del Campo. Environmental conditions and vegetation structure along this stretch are considered to be homogeneous [50]. Soils are luvisols and fluvisols [65] with a pH near 8 and a percentage of organic matter of 4.6–9 [61]. Altitude ranged from 554 to 602 m above sea level. Climate is continental Mediterranean with hot and dry summers and cold winters. Mean annual temperature and annual precipitation in the study area were 14.1°C and 528.5 mm in 2010 and 15.1°C and 411.1 mm in 2011 (Fig. 1) (data from “Torrejón de Ardoz” weather station, National Institute of Meteorology). In the study area the riparian forest is mostly constrained to a narrow band due to the occupation of floodplains by crops, industry and human settlements. The main dominant tree in the native community is Populus alba, which is accompanied by S. alba and to a minor extent, P. nigra, F. angustifolia and U. minor [50]. Among them, patches of the exotics A. altissima and R. pseudoacacia are frequent, mainly in human-disturbed areas [51]. P. alba has been found to be an exotic invasive species in other regions, such as Australia and South Africa [66], [67].

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Figure 1. Climatic conditions and plant phenology.

A) Climatic diagram showing monthly mean temperature and precipitation during 2011 (Torrejón de Ardoz weather station). B) Diagrams of phenological activity of the three species in 2011. Different letters across species for the beginning or end of each phenophase indicate significant differences after a pairwise Watson-Williams multisample test (P<0.05). Missing letters means that comparisons could not be performed for all species due the lack of variation between replicates.

https://doi.org/10.1371/journal.pone.0100254.g001

The three studied species, Populus alba, A. altissima and R. pseudoacacia, are considered as fast-growing pioneer trees which can reach up to 25 m of height [51], [52], [68], [69]. P. alba and A. altissima are dioecious, while R. pseudoacacia is monoecious but self-pollination is prevented by a hairy collar below the stigma [68]. The native P. alba is wind-pollinated [70] while the two exotics are pollinated by insects [51], [52]. Seeds are primarily dispersed by wind in the three species, although only P. alba and A. altissima have specific structures assisting wind-dispersal [52], [71]. While seed viability last only 2–4 weeks in P. alba, the two exotics are able to form seed banks, both in the soil and in the plant canopy [52], [72].

Phenological Monitoring

In January 2011, before any sign of budbreak, we selected 10–11 healthy adult trees per species to performed phenological observations. After flowering, we selected 5 and 8 additional trees in A. altissima and P. alba, respectively, to have representation of both sexes. Mean ± SE trunk diameter at breast height (DBH) of selected trees were 34.7±6.2, 28.0±3.3 and 33.5±4.0 cm for A. altissima, R. pseudoacacia and P. alba, respectively, which was representative of the average tree size in the studied populations. Monitoring of phenology spanned from 7th February 2011 until 12th April 2012, when fruit dispersal of A. altissima was completed. Every 1–3 weeks, depending on the activity of the plant, we noted whether the following phenophases were active or not: leaf development, flowering, fruit set, fruit dispersal, leaf abscission. Phenophases were considered active in a tree when they were easily observed at naked eye in at least 5% of the crown. These data are available at Table S1.

Branch Production

In January 2010 we selected 6 adult trees of R. pseudoacacia, 6 female and 6 male adult trees of A. altissima. This year we could not sample P. alba due to technical reasons. To minimize the risk of branch collection interfering with tree growth, in January 2011 we selected 6 new trees per species and sex plus 6 female adult trees of P. alba (the sex of the selected trees could not be identified before flowering, which occurred in the second sampling date, see Table 1). In every selected tree we measured DBH and performed 2–3 harvests of 5–10 two-year-old branches, coinciding with the time of maximum leaf, flower and fruit production (see Table 1). Branches were firstly dissected, separating current year (t) and previous-year (t-1) cohorts, and then each cohort was separated into different organs, oven-dried (>48 h at 60°C) and weighted (stem mass-S, leaf mass-L, inflorescence mass-FL and infrutescence mass-FR). To be representative of the full tree, current-year FL and FR values were multiplied by the proportion of branches in the canopy holding flowers or fruits. This proportion was estimated at the time of flower and fruit sampling as follows: we divided the canopy of each tree in four quarters and randomly selected 10 branches in each quarter. The proportion of the selected branches holding flowers/fruits was considered to be representative for the full tree. To correct for the allometric effects of previous-year shoot mass on current-year production, the oven-dried weight of every current-year fraction (St, Lt, FLt and FRt) was divided by that of the previous-year stem (St−1) bearing the fraction. In this way we obtained the relative production of stems (St/St−1), leaves (Lt/St−1), inflorescences (FLt/St−1) and infrutescences (FRt/St−1). In the case of P. alba, the six initially selected trees failed to produce fruits, so they were replaced by 6 additional trees from the same population for the last sampling of fruit production. These data are available at Table S2.

Secondary Growth

In February 2012, before the onset of the new growing season, 3–4 radial wood samples were extracted at 1.30 m of trunk height using an increment core borer (Haglöf, Sweden) from the same trees used in 2011 for branch production. Wood samples were glued on wooden holders and sanded until tree-rings were clearly visible under a binocular microscope. The width of the last two xylem rings, corresponding to 2010 (RW10) and 2011 (RW11), was measured with an accuracy of 1/100 mm using a LINTAB measuring table and the software TSAP [73]. At the same height where cores were extracted we measured trunk perimeter (P) to derive the trunk radius (R = P/2π) and assessed the average cork thickness (Cth) with a cork calliper (Haglöf, Sweden) in four equidistant points around the trunk. Using these data we estimated the trunk basal area in 2011 excluding the cork (BA2011 = π(R-Cth)2), the 2010 basal area (BA2010 = π(R-Cth-RW11)2 and the 2009 basal area (BA2009 = π(R-Cth-RW11-RW10)2). The basal area increment of each tree in 2010 and 2011 (ΔBAt) was the difference between current- and previous-year basal areas. These data are available at Table S3.

Statistical Analysis

Phenological data were analysed using circular statistics with Oriana 2.0 package. Dates of beginning and end of each phenophase (expressed as weeks since January the 1st and transformed into angles of a 52-week circle) were pair-wise compared across species using the multisample Watson-Williams F test [74].

In previous analyses we found that some growth variables differed between years in some species. For instance, in R. pseudoacacia a liner mixed model, using year as factor, DBH as covariate and tree as random factor, revealed that St/St−1 was significantly larger in 2010 than in 2011 (0.37 and 0.19 gg−1 respectively, t = 4.45, p = 0.002). Therefore, we decided to perform all analyses separately for 2010 and 2011. Although in 2010 we lack of native control, we decided to compare the two invasive species to check the extent to which the patterns found for them were consistent across years.

The allometric relations of St−1 with St, Lt, FLt and FRt were fitted using standard major axis estimation (SMA) after log-transforming both variables [75]. SMA lines were tested for different slopes between sexes in A. altissima and across species using the likelihood ratio statistic (LRS). When cross-species differences were detected, we performed pairwise comparisons, lowering the significance level to 0.017, following a Bonferroni correction for multiple comparisons [76]. When slopes did not differ across species, they were additionally tested for equal elevation and for shifts along the common axis, using the Wald test [75]. Analyses were performed using the “smart” package in R 3.0.2.

St, Lt, FLt, FRt,St/St−1, Lt/St−1, FLt/St−1 and FRt/St−1 were compared among species, using linear mixed models, with species as fix factor, DBH as covariate and tree as random factor. When species was significant, pairwise comparisons were performed using a Tukey post-hoc test. In the case of A. altissima, variables were also compared among sexes (fix factor), using DBH as additional fix factor and tree as random factor. When necessary, variables were log-, 0.25- or 0.01-power-transformed to achieve homoscedasticity and normal distribution of residuals. Analyses were performed using R 3.0.2 and packages “nmle” and “multcomp”.

To assess the relative investment in vegetative growth vs. sexual reproduction, we represented in a plane defined by the mean vegetative (leaves+stems) and reproductive (inflorescences+infrutescences) mass produced per previous-year stem, each species, sex (in the case of A. altissima) and each year. Species above the 1∶1 line had a relative higher investment in sexual reproduction while species below this line invested more in vegetative growth.

The allometric relation between basal area increment (ΔBAt) and previous-year basal area (BAt−1) was fitted using standard major axis estimation (SMA). SMA lines were tested for different slope, elevation and shift across species as explained above.

Results

Phenological Differences Across Species

Vegetative growth occurred mainly in spring in the three species, largely coinciding with the months of higher precipitation (Fig. 1). Although the three species started at a similar date, A. altissima arrested growth significantly earlier than R. pseudoacacia and P. alba (Fig. 1). Flowering phenology largely contrasted across species, being the earliest in P. alba (March), intermediate in R. pseudoacacia (late April–mid May), and the latest in A. altissima (June) (Fig. 1). Accordingly, fruit set was earlier and shorter in P. alba (April), followed by R. pseudoacacia (May–September) and by A. altissima (June–October) (Fig. 1). Leaf abscission occurred mostly in autumn, coinciding with the temperature drop, although A. altissima started later (early October), P. alba ended later (late December), and R. pseudoacacia was intermediate (Fig. 1). Finally, fruit dispersal occurred all over the year in the invaders, while in P. alba it was concentrated between April and May (Fig. 1).

Branch Production

In A. altissima the slope of the allometric lines did not differ between sexes (LRS<0.5 and P>0.36 in all cases), but the LtSt−1 line showed higher elevation in females, whereas the FLt- St−1 line showed higher elevation in males (Fig. S1). Similarly, FLt/St−1 tended to be marginally higher in males (linear mix model effect F-value for sex = 3.38, P = 0.10, Table S4). This means that, for a given unit of previous year stem, females produce more leaf mass but less flower mass than males. The rest of variables were not affected by sex (Table S4).

The mass of every branch organ in either cohort was the largest in A. altissima and the smallest in P. alba, although FLt and FRt did not differ between A. altissima and R. pseudoacacia in 2011 and 2010, respectively (Table 2). However, when organ mass was relativized by St−1, A. altissima tended to show larger relative production only for stems, but lower for reproductive organs (Fig. 2 right, Fig. S2). In 2011, linear mixed models showed that St/St−1 was the largest in A. altissima and similar between P. alba and R. pseudoacacia (Fig. 2B). Lt/St−1 was similar across species, being marginally smaller in R. pseudoacacia than in P. alba (P = 0.07). By contrast, FLt/St−1 and FRt/St−1 followed the ranking R. pseudoacaciaP. albaA. altissima (Fig. 2F, H). This ranking was consistent independently of whether any or both sexes were considered in A. altissima (data not shown). In 2010 comparisons between A. altissima and R. pseudoacacia showed similar trends, but less often significant (Fig. S2). Among all analyses, DBH was only significant in the case of St/St−1 of 2011, where it showed a negative slope (i.e. thicker trees tended to produce less stem mass). In 2011, the random factor (tree) explained between 37 and 57% of the variance not explained by the fixed factors, while in 2010 this proportion varied from 6% to 83% (Table 3).

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Figure 2. Species branch production in 2011.

Left: Allometric relationships between previous-year stem mass and current year stem (A), leaf (C), inflorescence (E) and infrutescence (G) mass (note the log-scale of both axes). The Likelihood Ratio Statistic (LRS) comparing slopes across species and its significance is shown in each chart. Different letters indicate significant slope differences (P<0.017, after Bonferroni correction). Right: relative production of current-year stem (B), leaf (D), inflorescence (F) and infrutescence (H) mass, expressed per unit of previous-year stem mass. Different letters across species indicate significant differences (Tukey test, see Table 3 for more details).

https://doi.org/10.1371/journal.pone.0100254.g002

The allometric relationships revealed that species differed in the gain of current year organ mass per unit of St−1 increase. In the case of St and FLt, this gain was the largest in A. altissima and the smallest in P. alba (see slopes in Fig. 2A and 2E). In the case of Lt, the gain was the lowest in P. alba, which showed the smallest slope, R. pseudoacacia showing larger slope than A. altissima (Fig. 2C). In the case of infrutescences, slopes of SMA lines were similar across species, although A. altissima exhibited smaller elevation than the other species (Wald statistic = 52.3 P<0.001), i.e. this species produced less infrutescence mass for a given value of previous-year stem mass (Fig. 2G).

The relationship between vegetative and reproductive mass per previous-year stem was c.a. 1∶1 in female P. alba trees of 2011. R. pseudoacacia invested more in sexual reproduction, either in 2010 and 2011, while A. altissima invested more in vegetative growth, disregard the sex and the year (Fig. 3).

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Figure 3. Relationship between vegetative and reproductive production.

Relationship between mean (±SE) vegetative mass (VG = leaf+stem mass) and the mean (±SE) reproductive mass (RP = inflorescence+infrutescence mass) produced per previous-year stem in each species (AA- Ailanthus altissima, RP- Robinia pseudoacacia, PA- Populus alba), each sex (f-female, m-male) and each study year (2010 and 2011). The dashed line indicates the 1∶1 relation between VG and RP. Species lying above this line invested more in reproduction, while species below the line invested more in vegetative growth. (Note the log-scale of both axes).

https://doi.org/10.1371/journal.pone.0100254.g003

Secondary Growth

The annual increment of trunk basal area allometrically increased with previous-year basal area in all species (Fig. 4). The slope of SMA lines tended to be smaller for A. altissima than for the remaining species, but this difference was marginally significant in 2010 and non-significant in 2011 (Table 4). The elevation of the SMA line was significantly smaller for R. pseudoacacia than for P. alba (Fig. 3, Table 4), which means that, at equal previous-year basal area, R. pseudoacacia increased less its basal area than P. alba. Ailanthus altissima did not differ in the elevation of its SMA line from the remaining species. Results were consistent between years (Fig. 4, Table 4).

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Figure 4. Tree basal area increment.

Allometric relationship between current-year basal area increment and previous-year basal area for 2010 (left) and 2011 (right). Each dot corresponds to a different tree. Different letters indicate significant difference of elevation across species (pairwise Wald test for multiple comparison, P<0.017, after Bonferroni correction). Slopes did not significantly differ across species (see Table 4).

https://doi.org/10.1371/journal.pone.0100254.g004

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Table 4. Comparison of the allometric relation between trunk growth and area across species.

https://doi.org/10.1371/journal.pone.0100254.t004

Discussion

Is there a Phenological Offset between the Invaders and the Native?

We expected A. altissima and R. pseudoacacia to differ from P. alba in phenology (hypothesis 1), as a mechanism to exploit temporal empty niches, to reduce competition and to promote coexistence [22], [23], [25], [30], [77]. This hypothesis was only supported for reproductive growth, which occurred later and during a longer period in the invaders than in the native tree (see Fig. 1). Given that flowering and fruiting phenology –at monthly resolution– where available in the literature for other native woody species, we also compared the invaders phenology with that of the most frequent native woody plants of the studied floodplains. Most native trees (i.e. Populus nigra, Salix alba and Ulmus minor) show a reproductive phenology similar to P. alba (early flower and fruit set), only F. angustifolia showing a pattern in between P. alba and the invaders (early flower but autumn fruit ripening [70]). By contrast, the reproductive phenology of the tree invaders resembles more to that of several native entomophilous riparian shrubs / vines, such as Cornus sanguinea L., Humulus lupulus L., Ligustrum vulgare L. or Rubus ulmifolius Schott, which flower from April/May to July/August, and extend fruit set until autumn [70].

The period when a plant flowers is determined by the interaction between genetic factors and environmental variables (e.g. water availability, temperature or day length) [78][81], or pollinator phenology [82], [83]. An evidence of the genetic control of flowering time is the fact that invasive plants tend to keep their native flowering phenology after being transferred to a climatically different region [77]. One genetic factor that constrains flowering phenology is the architecture of buds [84]. Species which bloom before leafing-out, such as P. alba, necessarily possess separated reproductive (R) and vegetative (V) buds, which allows any combination of vegetative and reproductive phenology [84], [85]. By contrast, A. altissima and R. pseudoacacia enclose vegetative and reproductive primordia within the same buds (VR buds), which forces these species to start shoot elongation and leaf expansion before bloom [84] (see Fig. S3). Among environmental factors, summer drought is a strong selective force for phenology in Mediterranean regions, where flowering mostly occurs in spring [84], [86][88]. However, the presence of a water table in the floodplains probably makes this factor less limiting, since late-spring/summer flowering is not uncommon among native undercanopy shrubs and vines of the studied region [70]. If the climatic filter is not so strong, pollination type may be a more relevant driver for flowering phenology. Wind-pollinated plants, such as P. alba, and the other native trees (P. nigra, S. alba, U. minor and F. angustifolia) [70], have been found to bloom earlier than animal-pollinated plants [83], as wind pollination is more efficient when plants are still bare of leaves. By contrast, A. altissima and R. pseudoacacia, like the above mentioned native shrubs/vines, are insect-pollinated and present late flowering [52][54], [70]. In this case, a successful pollination requires blooming to occur at a time with high insect activity, which coincides with the months of warmer temperature [89], [90].

What are the Benefits of an Offset Phenology for the Invaders?

The niche for insect-pollinated late-flowering trees seems to be empty in the studied native community and successfully colonized by A. altissima and R. pseudoacacia. The invaders may benefit from the reproductive asynchrony with native trees due to a reduced competition for the resources demanded for flowering and fruit set, i.e. nutrients, water and pollinators, which in A. altissima and R. pseudoacacia are mainly honeybees, beetles and other nectar- and pollen-feeding generalist insects [52], [68], [91]. In fact, a shortage of pollinators has been found to limit fruit production in many invasive plants, including R. pseudoacacia [92][94]. Given that all native trees in the recipient community are wind-pollinated [70], A. altissima and R. pseudoacacia only have to face competition for pollinators by the sub-dominant undercanopy entomophilous shrubs and vines. Moreover, the latter species may facilitate exotic tree invasion by providing an established pollinator community, as found before [93], [95]. A. altissima and R. pseudoacacia also contrasted with P. alba in the year-round seed dispersal period. Seed viability is very short in P. alba and the remaining native trees of the studied community [70], [72], [96] but it extends for one year or more in the invaders, which allows them to form seed banks, both in the canopy and in the soil [97][100]. In this way these species widen the temporal window for sexual reproduction and reduce seed loss in years with poor conditions for germination [101], [102]. Another remarkable difference between the invaders and the native is the longer fruit setting period of the former. Our hypothesis 2 predicted that a more extended organ phenology would allow a higher relative production of that organ. However, against this prediction, we found that the relative fruit production of P. alba was not significantly different from any of the invaders (Fig. 2H). Alternatively, previous studies found a relation between fruit set duration and seed mass [103]. This was true for our species, as seed mass of the long fruit-set invaders (A. altissima = 14–25 mg, R. pseudoacacia = 16–20 mg [104]) were almost two orders of magnitude larger than the short fruit-set native P. alba (0.1–0.6 mg [70], [72], [104]). Large seeds have been found to produce larger seedlings, to have more chances to grow through the thick debris layer of deciduous forests, and to expand leaves earlier –before canopy closure-, therefore having more chances to survive in the undercanopy [105]. Although A. altissima and R. pseudoacacia possess many traits of pioneer trees and gap-colonizers [52], [68], [71], [104], their larger seeds may make them less dependent on gaps for sexual reproduction than the tiny-seeded P. alba. Moreover, gaps in riparian forest are becoming less frequent due to flow regulation, which decreases the frequency of gap-opening extraordinary floods [48], [49] and expands the potential regeneration niche for large-seeded plants. Most of the remaining native trees of the native community are also tiny-seeded (e.g. P. nigra, S. alba and U. minor) and only F. angustifolia possesses seeds larger than those of the invaders [70], [72], [104]. By contrast, other exotic trees, such as Acer negundo L. and Elaeagnus angustifolia L, which are potentially invasive in Spanish riparian forests [51], also possess large seeds [104]. Therefore native tree richness of the studied riparian forest might decline in the future at the expense of exotic tree richness if the dynamic of regulated rivers is not restored.

Vegetative and Reproductive Growth of Invasive and Native Trees

Branch gross production was the highest and the lowest in A. altissima and P. alba, respectively, and intermediate in R. pseudoacacia, either for stems, leaves, inflorescences or infrutescences (Table 4). This result suggests a clear competitive advantage of the invaders (especially A. altissima) over the native at the adult stage. However, when plant production was relativized per unit of previous-year stem mass (i.e., relative production), differences between R. pseudoacacia and P. alba tended to disappear (vegetative and reproductive relative productions were marginally lower and higher in R. pseudoacacia, respectively) and A. altissima only retained a superior relative production for stems but showed the lowest reproductive relative production (Fig. 2B, F, H). Moreover, secondary growth relativized per unit of pre-existing basal area was the lowest in R. pseudoacacia and similar between A. altissima and P. alba (Fig. 4). This suggests that the observed superior gross production of the invaders was due to a larger pre-existing biomass, rather than to a faster growth, and the larger pre-existing biomass may be due to a faster growth at early ontogenetic stages. In line with this argument, annual tree height increment of A. altissima in different parts of the invaded range has been reported to be as large as 2 m in 1–2 year-old seedlings growing under favourable conditions [106], [107] and to decline to 8 cm or less in 20–25 year-old trees [52]. Similarly, in its native area R. pseudoacacia, has been reported to show a fast growth rate in the early life stages which sharply decreases at an age of only 10–20 years [71].

The larger relative production of stems (Lt/St−1) found in A. altissima agrees with the above reports of great potential for height growth [106], [107]. Moreover, the trend of A. altissima to have a less steep allometric relation between basal area increment and trunk area than the other species (see slopes in Fig. 4), suggests that A. altissima prioritize primary over secondary growth. Such a strategy may help this species to quickly overtop other coexisting plants and thus to escape from the canopy shade that it cannot tolerate [52], [108]. One functional property behind the high growth potential of A. altissima may relate to its ability to optimize light capture by combining physiological attributes of high- and low-irradiance adapted plants [109]. The low vegetative relative production of R. pseudoacacia was observed for both leaf and stem mass production (Fig. 2B, 2D), as well as for secondary growth (Fig. 4). This unexpected result for a species which is considered as a successful invader in Spain [51] may be attributed to different non-exclusive reason. First, in the study region R. pseudoacacia usually shows open crowns, sparse leaves and many dead branches, suggesting that trees are affected by any disease [110]. Second, given the positive association between secondary growth and April-June precipitation [111], the drastic summer decrease of rainfall typical of Mediterranean regions (see Fig. 1) may hamper the realization of its potential growth. Third, R. pseudoacacia growth rate may drastically decline with tree age according to the short-life and pioneer strategy described for this species [68], [71], so that the relative low growth capacity observed may be an ontogenetic effect. By contrast, this species exhibited the largest relative reproductive production, which may contribute to a great potential to spread.

Our third hypothesis predicted lower ratio of reproductive to vegetative annual mass production in the invaders than in the native as a pre-adaption to the decreased rate of gap formation resulting from flood regulation. In P. alba, this ratio was close to 1∶1 for female trees, although we can expect it to be slightly lower for males, as found in other dioecious species [39]. According to our hypothesis, A. altissima showed the lowest ratio, indicating that vegetative growth was prioritized over sexual reproduction, and in line with the great growth potential reported for this species [106][108]. However, contrary to our hypothesis, R. pseudoacacia showed the highest reproductive to vegetative mass ratio, which suggests a great potential to colonize new sites, but not so great to compete with other plants. The prioritization to sexual reproduction found in R. pseudoacacia is consistent with reports indicating that vegetative growth notably declines after 10–20 years [71], while fecundity might not decline before 30–40 years [94].

Altogether, our growth results suggest that both A. altissima and R. pseudoacacia may outcompete the native P. alba due to their large mass acquired at early stages. Later on, A. altissima would retain a great height growth capacity, which would allow it to overtop other coexisting plants. However, R. pseudoacacia would suffer a rapid decline in vegetative growth rate, but retain a high potential for fruit production which would make this species more efficient to colonize new sites than to compete with coexisting adult trees of P. alba.

Conclusions

Ailanthus altissima and R. pseudoacacia show reproductive traits (late flowering phenology, insect-pollination, long fruit set period, relatively large seeds) different to those of the tree species dominating the studied riparian forest community of central Spain. These differences may help them to occupy an empty reproductive niche, and then benefit from a reduced competition for the resources required by flower/fruit development and for pollinators. Moreover, the long fruit set period of invaders and the associated larger seed mass may make them less dependent on gaps for seedling establishment than the tiny-seeded native tree P. alba. Such ability may help the invaders to expand their reproductive niche in flood-regulated rivers of the study region. The two invasive species showed a superior gross production than the native which was due to a higher size of pre-existing stems rather than to a faster relative growth rate. At the studied adult stage A. altissima and R. pseudoacacia showed the lowest and highest reproductive/vegetative mass ratio, respectively. Therefore, A. altissima might outcompete the native trees thanks to a higher vegetative growth whereas R. pseudoacacia may do so by means of a higher investment in sexual reproduction. Altogether, our findings suggest that preserving the natural flood regime of the river is the best strategy to favour the native trees over the two invaders in this case study. Given the great vegetative production of the invaders at adult stage, especially in the case of A. altissima, an efficient management should focus on early detection and prevention of establishment. Further information on the growth potential of all native and invasive tree species at different ontogenetic stages would help to predict the dynamics of this invaded riparian forest community.

Supporting Information

Figure S1.

Allometric relationships between previous-year stem mass and current year leaf (left) and inflorescence (right) mass in A. altissima (data of 2011). Different symbols and lines represent different sexes. The Wald statistic indicates a significant difference in line elevation. No significant difference was found between slopes.

https://doi.org/10.1371/journal.pone.0100254.s001

(TIF)

Figure S2.

Left- Allometric relationships between previous-year stem mass and current year stem (A), leaf (C), inflorescence (E) and infrutescence (G) mass (note the log-scale of both axes). The Likelihood Ratio Statistic (LRS) comparing slopes across species and its significance is shown in each chart. When slopes were equal, we also show results for the Wald satistics (WS) comparing elevation and shift along a common slope. Right- current-year stem (B), leaf (D), inflorescence (F) and infrutescence (H) mass per unit of previous-year stem mass. Different letters across species indicate significant differences (Linear mixed model, species and DBH being the fix factors and tree the random factor). Data from 2010 collection.

https://doi.org/10.1371/journal.pone.0100254.s002

(TIF)

Figure S3.

Left- Picture of a current-year shoot of Ailanthus altissima, showing that the same current-year stem bears leaves and inflorescences, which were derived from the same VR bud (see text) (April 2009). Centre- Picture of two recently opened buds of Robinia pseudoacia. It can be observed that both leaves and inflorescence derived from the same VR buds (March 2009). Right- Picture of a female inflorescence of Populus alba. It can be seen that the inflorescence grew on the previous-year stem from a R bud, while the apical vegetative (V) bud, enclosing the current-year vegetative organs, is still close (March 2009). Pictures by the authors.

https://doi.org/10.1371/journal.pone.0100254.s003

(TIF)

Table S1.

Phenological data of the studied species.

https://doi.org/10.1371/journal.pone.0100254.s004

(XLSX)

Table S2.

Branch production data of the studied species.

https://doi.org/10.1371/journal.pone.0100254.s005

(XLSX)

Table S3.

Secondary growth of the studied species.

https://doi.org/10.1371/journal.pone.0100254.s006

(XLSX)

Table S4.

Comparison of shoot production between sexes in Ailanthus altissima.

https://doi.org/10.1371/journal.pone.0100254.s007

(DOC)

Acknowledgments

We acknowledge the Spanish Meteorological National Agency (AEMet) for freely supplying the climatic data shown in Fig. 1A. According to the Order MAM/160/2006 of January the 2nd, these data have a value of 21.77 euros.

Author Contributions

Conceived and designed the experiments: PCD AA NGM. Performed the experiments: GV NGM. Analyzed the data: PCD. Contributed to the writing of the manuscript: PCD. Critical review of the draft: AA GV NGM.

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