The LH levels in the PKR2 antagonist (10 mg/kg 3Cl-MPL) group were significantly reduced compared to the vehicle treatment (n = 6 for each group, p<0.01.
PKR2-GFP expression was shown in green and ERα expression was shown in red. Yellow or orange color in the MnPO and AVPV regions indicates likely co-expression of PKR2 and ERα (scale bars:100 µm).
Antagonist potency was examined in Chinese Hamster Ovary (CHO) cells that stably express PKR2. RLU is an index for calcium influx measurement with a luminescence-based assay. The IC50 of 3Cl-MPL for PKR2 were 24.9±4.3 nM(Mean ± S.E.). Shown was representative of three independent experiments.
Citation: The PLOS ONE Staff (2014) Correction: Signaling Role of Prokineticin 2 on the Estrous Cycle of Female Mice. PLoS ONE 9(5): e98314. doi:10.1371/journal.pone.0098314
Published: May 15, 2014
Copyright: © 2014 The PLOS ONE Staff. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.