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Correction: SinR Controls Enterotoxin Expression in Bacillus thuringiensis Biofilms

  • The PLOS ONE Staff

Correction: SinR Controls Enterotoxin Expression in Bacillus thuringiensis Biofilms

  • The PLOS ONE Staff
PLOS
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The figure legends for Figure 4 and Figure 5 contain misnumbered subfigures in the PDF and XML versions of the article. Please see these figures and their corrected figure legends below.

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Figure 4. Heterogeneity of hbl expression in planktonic cultures and in biofilms.

A: Flow cytometry analysis of bacteria expressing Phbl'-yfp in planktonic cultures or in biofilms, shown as histogram plot. The blue-filled curve shows biofilm data, the yellow-filled curve shows planktonic cultures data and the unfilled dashed curve shows data from bacteria lacking yfp. B: Flow cytometry analysis of bacteria expressing Papha3'-yfp in biofilms (blue-filled curve) compared to bacteria lacking yfp (unfilled dashed curve), shown as histogram plot. C: Expression from the hbl promoter was monitored in planktonic cultures and in biofilms by epifluorescence microscopy through a transcriptional fusion to yfp. Cell limits are shown by the membrane stain FM4-64 (red).

https://doi.org/10.1371/journal.pone.0087532.g004

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Figure 5. Expression of hbl and of sinI in biofilms.

A: Flow cytometry analysis of bacteria expressing Phbl'-yfp and PsinI'-mcherry in 48 h-old biofilms, shown as dot-plot. While 72% of the bacteria do not express hbl nor sinI (quadrant d), 15% of the cells which express hbl also express sinI (quadrant b), and 12% of the bacteria express sinI but nothbl (quadrant a). B: Observation by epifluorescence microscopy of bacteria expressing Phbl'-yfp (left, in green) and PsinI'-mcherry (center, in red) in 48 h-old biofilms. An overlay of YFP fluorescence (hbl expression), mCherry fluorescence (sinI expression) and phase contrast microscopy is shown on the right.

https://doi.org/10.1371/journal.pone.0087532.g005

Reference

  1. 1. Fagerlund A, Dubois T, Økstad O-A, Verplaetse E, Gilois N, et al. (2014) SinR Controls Enterotoxin Expression in Bacillus thuringiensis Biofilms. PLoS ONE 9(1): e87532