Coloration of stinging insects is often based on contrasted patterns of light and black pigmentations as a warning signal to predators. However, in many social wasp species, geographic variation drastically modifies this signal through melanic polymorphism potentially driven by different selective pressures. To date, surprisingly little is known about the geographic variation of coloration of social wasps in relation to aposematism and melanism and to genetic and developmental constraints. The main objectives of this study are to improve the description of the colour variation within a social wasp species and to determine which factors are driving this variation. Therefore, we explored the evolutionary history of a polymorphic hornet, Vespa velutina Lepeletier, 1836, using mitochondrial and microsatellite markers, and we analysed its melanic variation using a colour space based on a description of body parts coloration. We found two main lineages within the species and confirmed the previous synonymy of V. auraria Smith, 1852, under V. velutina, differing only by the coloration. We also found that the melanic variation of most body parts was positively correlated, with some segments forming potential colour modules. Finally, we showed that the variation of coloration between populations was not related to their molecular, geographic or climatic differences. Our observations suggest that the coloration patterns of hornets and their geographic variations are determined by genes with an influence of developmental constraints. Our results also highlight that Vespa velutina populations have experienced several convergent evolutions of the coloration, more likely influenced by constraints on aposematism and Müllerian mimicry than by abiotic pressures on melanism.
Citation: Perrard A, Arca M, Rome Q, Muller F, Tan J, Bista S, et al. (2014) Geographic Variation of Melanisation Patterns in a Hornet Species: Genetic Differences, Climatic Pressures or Aposematic Constraints? PLoS ONE 9(4): e94162. https://doi.org/10.1371/journal.pone.0094162
Editor: Alexandre Roulin, University of Lausanne, Switzerland
Received: September 19, 2013; Accepted: March 11, 2014; Published: April 16, 2014
Copyright: © 2014 Perrard et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was funded by two ATM (“Formes possibles, formes réalisées” and “Biodiversité actuelle et fossile”) from the Museum National d'Histoire Naturelle, the Bourse Eole of the Franco-Netherland alliance and the Bourse Germaine Cousin of the Société Entomologique de France. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Geographic variation of coloration is one of the most striking aspects of diversity within many species , . Numerous factors could influence this colour variation, including evolutionary drift , but also various selection forces triggered by environmental differences between populations , . One of the factors of selection on coloration is aposematism, the use of a highly visible signal to warn predators of unpalatability –, such as the black and light stripes of wasps –. Efficiency of aposematic signals relies on the frequency of this signal in the habitats and its probability to be experienced by predators , , . Therefore, it may seem counter-intuitive to find colour diversity under aposematic constraints. However, colour variation occurs in many aposematic species –. In these species, selection on aposematic signals can also interact with other selective pressures for driving the colour variation in the species. For example, the main colour variation within wasp species is a change in the degree of dark pigmentation, or melanisation, inducing light- or dark-coloured morphs and confusing the taxonomy of the group , , . This melanism is known to be of adaptive importance in insects: it has been related to crypsis, to thermoregulation and to the resistance to pathogens –. Selection on melanism can thus interact with selection on aposematic patterns of coloration . Surprisingly, the potential relations of intra-specific colour variation with aposematism and melanism have been rarely studied in social wasps (but see ). In this study, our aim is to determine which factors influenced the geographic variation of coloration within a species of wasp.
In many organisms, melanism is expressed by a roughly homogeneous increase of the melanin pigmentation over the body , . On the contrary, in aposematic insects such as wasps and ladybirds, contrasted colours are a major component of the warning signal. In these organisms, melanism occurs through an increase of the area of delimited melanic patterns on the body , , . We thus described the variation in coloration of the species by the variation of these melanic patterns.
Warning signal in wasps includes the colour patterns of the different body segments. In order to maintain a recognizable warning signal among the different colour variants, the variation of body parts may follow an integrated process over the entire body, i.e. the different segments would vary in a coordinated way, thus maintaining the unity of the general pattern , . Furthermore, the different body parts are not likely to play an equivalent role in the warning signal. The coloration of some body parts may be either affected by stronger selective pressures or be under the influence of the same developmental pathways. These phenomena would result in modularity , i.e. some body parts co-varying more between them than with the other parts, forming colour “modules”. Integration and modularity may enhance the quality of the warning signal of the different colour variants by conserving a coherent pattern between the different body parts involved in this signal.
The evolutionary history of the species is also a major component in understanding the colour variation. In case of highly convergent variation in distantly related populations, the melanism variation could be induced by selective pressures caused either by abiotic factors related to climate, or biotic factors related to aposematism. If melanism convergence was caused by abiotic factors, melanisation should present geographical or altitudinal clines related to climatic proximity . On the other hand, the geographical variation of coloration driven by aposematic pressures would more likely reflect a mosaic of locally selected phenotypes than a cline .
We studied the colour variation of the yellow-legged hornet Vespa velutina Lepeletier, 1836 which presents a dozen distinct colour morphs across its distribution in South-East Asia , ,  (Fig. 1). Its coloration varies among populations from almost entirely yellow or orange to extensively black , , and can be labile within populations. This phenomenon underlay previous distinction of a second species, Vespa auraria Smith, 1852, because of the apparent sympatry in different localities of this colour form with another form called nigrithorax , .
In order to assess the evolutionary history of the species across its distribution and to disentangle potential taxonomic differences that could bias the analysis, we first explored the genetic relatedness of Vespa velutina populations using mitochondrial DNA sequences and microsatellite markers. Then, to quantify the colour variation of the individuals, we built a “colour space” from the gradual colour variation between populations based on the measure of melanic patterns of the different body parts. We tested the integration of the melanic variation and its modular nature through the correlation of the body parts vectors in the colour space. Finally, we tested whether the colour variation across the species was congruent with the genetic variation, geographic clines or a spatial mosaic by comparing geographic, genetic and phenotypic distances. These analyses allowed us to answer the following questions:
- Is auraria a lineage genetically divergent from the other colour morphs of V. velutina?
- Is the colour variation of hornet well depicted by a “colour space” based on melanic patterns?
- Was the coloration of an aposematic species influenced by developmental processes such as integration and modularity?
- Was the colour variation driven by genetic variation or differences in climatic niches?
We then discussed the implications of our results for the understanding of the development and variation of colour patterns in social wasps.
Material and Methods
A total of 448 specimens of Vespa velutina from 216 localities were studied, including 125 recently collected specimens from 69 different localities from Nepal, China, Laos, Thailand, Vietnam and six Indonesian islands. Other specimens used in this study were assembled from public and private collections. No endangered or protected species were sampled for this study. Specimens sampled in Indonesian and Vietnam were collected partially in national parks with the corresponding authorizations: the research permits were obtained from the head office of the forest protection and nature conservation in Indonesia and from the Tropical Institute for Biology in Vietnam; the permits to collect in National Parks (NP) were delivered by the local authorities of Gunung Rinjani NP in Lombok (Indonesia), of Laiwangi Wanggameti NP in Sumba (Indonesia) and of the Bidoup-Nui Ba NP in Vietnam. The localities sampled in other countries were not protected in any way. No specific permits were required for these collections made in collaboration with local researchers and authorizations of the land owners. Indonesian vouchers were stored at the Museum Zoologicum Bogoriense. Other vouchers were housed in the Museum National d'Histoire Naturelle in Paris. The study of public collection specimens was allowed by curators Y. Gérard and A. Drumont (Institut Royal des Sciences Naturelles, Brussels), J. van Achterberg (Nationaal Natuurhistorisch Museum Naturalis, Leiden), G. Broad (Natural History Museum, London) and F. Gusenleitner (Oberösterreichischen Landesmuseen, Linz). The study of private collection specimens was granted by their owners J. Gusenleitner, J. Haxaire, J.-L. Renesson and P. Tripotin.
Over the 216 studied localities, populations were delineated based on colour morphs, geographic distances and ecological environments. We defined ten continental populations (Kashmir, Nepal, Yunnan, Zhejiang, Guangdong, north of Vietnam, south of Vietnam, Thailand, Kra and Malaysia), and eight insular populations from Taiwan and nine Indonesian islands (Sumatra, Java with Bali, Lombok with Sumbawa, Flores, Sumba, Sulawesi and Timor) (Fig. 2, Table 1). Four populations (Kashmir, Nepal, Thailand and Yunnan) each encompass two to three colour morphs for which colour distributions are known to overlap in these areas (Fig. 1). The delineation of these populations was based on the geographic distances of collected individuals and altitude. For example Yunnan specimens were defined as specimens from the mountains while Thailand specimens were defined as located at lower altitude in the North Indo-Burman valley. Three closely-related species were used as outgroup: V. bicolor Fabricius, 1787, V. simillima Smith, 1868, and V. vivax Smith, 1870 .
Dotted populations are represented by less than 10 specimens.
DNA extraction, Polymerase Chain Reaction, Sequencing and Genotyping
Recently collected specimens were preserved in 95% ethanol. Genomic DNA was extracted from legs using QIAGEN ‘DNeasy tissue Kit’.
A 658 bp sequence of the mitochondrial gene cytochrome C oxidase subunit I (CO1) was amplified for 119 specimens of V. velutina, one of V. bicolor, one of V. vivax and one of V. simillima using universal primer sequences HCO and LCO . DNA amplification followed the standard Polymerase Chain Reaction (PCR) protocol of the Canadian Centre for DNA Barcoding . PCR products were checked on a 2% agarose gel. The purified PCR products were sequenced in both directions. BIOEDIT 220.127.116.11 and CodonCode Aligner V.3.5 were used to align both strands of DNA . Sequences were truncated to the same length (658 bp) to avoid missing data. No insertions, deletions or stop codons were found in the alignment.
Genotypes of V. velutina populations were assessed using 11 of the 15 microsatellite loci previously developed for the analysis of the origin of the invasion of V. velutina in France: D2-185, R4-100, R4-114, D3-15, R1-36, R1-75, R1-77, R4-33, R1-137, R1-169 and D2-142 , as well as two supplementary loci (List 2015 and List 2020B) . PCR protocols and genotype scoring are detailed by Arca . Because of high frequencies of missing values in the sample caused by low-quality template DNA, the other loci used in Arca et al.  were not analyzed.
Haplotype network and diversity among CO1 sequences were calculated using NETWORK 18.104.22.168 software . An evolutionary tree based on CO1 sequences was computed using the Maximum Likelihood criterion (ML) under a GTR model with PhyML 2.4.4 . Bootstrap supports were calculated from 1000 resamplings.
We built a Neighbor Joining (NJ) tree  of individuals using (microsatellite) shared allele distances (DAS; ) with the R software and “ape” library , and performed a Principal Coordinate Analysis (PCoA) on these distances. Population structure was explored using Bayesian clustering through STRUCTURE 2.0 software . We used admixture model and correlated frequencies. Length of the burnin and the MCMC were 10.000 and 100.000 respectively. Simulations were iterated 10 times for each number of clusters from K = 2 to K = 15. Specimen assignment to a cluster was defined by the probability of the specimen to belong to this cluster with a threshold of 0.5.
Descriptive statistics of populations based on microsatellite data as the genetic diversity estimate θ were obtained using Arlequin 3.01 .
Colour pattern analyses
The curved nature of most body segments of wasps makes a standardized quantification of colour over the insect body difficult, due to reflectance and luminosity problems caused by the 3D structure. Furthermore, colour of specimens in natural history collections can be altered by the different collecting methods and conservation conditions. Direct colour quantification using common photograph and pixel-based methods are hardly suitable to such analyses. On the other hand, a semi-quantitative characterisation of melanin patterns can be applied.
We identified the melanic patterns, or modalities, of the different body parts using the original descriptions of V. velutina colour morphs and the observation of 448 collection specimens. Body parts with constant coloration (e.g. clypeus, propodeum), rare colour variation present in less than 5% of the specimens studied (e.g. middle- and hind-femora) or variation without clear patterns (e.g. coxae) were not included in the analysis.
We found 23 recurrent colour variations over the body parts, four concerning the head, five the mesosoma, three the legs, seven the metasomal dorsum and four the metasomal sterna (Table 2, Fig. 3). These characters were coded in two to five modalities, giving a total of 73 patterns depicted in Figure 4 (cf. Appendix S1). For each analysed body part, extreme melanic patterns were coded as two binary characters of the presence/absence of the darkest and the lightest modalities. Intermediate modalities were coded following a fuzzy coding between these two extreme characters . In practice, it is equivalent to coding the modalities following their ranking in an ordination of the modalities from one extreme to the other as:where r is the rank of the coded modality, starting from 0 for the extreme modality not described by the character, for example the darkest modality for a character of presence/absence of the lightest modality, and n the total number of modalities.
Characters coding variation of melanisation are in bold and numbered from one to 23. The twelfth character (spot at the apex of the hind-tibia) is not depicted.
Each colour character was depicted independently from the others. The ensemble of colour patterns gathered in a given illustration is thus not necessarily reflecting an actual coloration found in wild organisms. A. Variation of the four head characters in dorsal view. B. Variation of the four dorsal mesosomal characters. C. Variation of the lateral mesosoma character and the two anterior leg characters. The hind leg with a spot at the apex of the hind-tibia was not depicted. D. Variation of the seven dorsal metasomal characters. E. Variation of the four ventral metasomal characters.
Only workers were coded in order to avoid bias caused by social colour dimorphism within the species .
We analysed the resulting matrix of 46 variables ranging from 0 to 1 using a correspondence analysis (CA). The coding in two opposed variables for each character induced a marginal value identical for every individual, independently from their melanisation level, and so the same weight in the CA. In such analysis, colour variation is estimated by semi-quantitative variables describing the level of melanisation of each body part. Because the modalities of a given body part could also be considered as independent discrete states between populations, we also computed a multiple correspondence analysis (MCA) on a matrix considering each modality as a variable. The second analysis being based on 73 variables, its description of colour variation was seemingly more precise than the one using the fuzzy coding. However, the CA with fuzzy coding has the advantage of describing the variation of melanic patterns of each body part by a linear direction in the resulting multivariate “colour space”. In the MCA, the variation of a single body part is described by a succession of vectors, due to the different modalities.
In order to quantify the accuracy of the colour spaces from the two analyses, we compared the distribution of individuals in these spaces to the classes of colour morphs distinguished by systematists. We tested for the separation of individuals from these different morphs in the colour spaces using cross-validation from a canonical variate analyses.
Both light and dark characters of a body part being vectors with opposite directions from the origin in the CA, we estimated the directions of variation of melanic patterns in the colour space by focusing only on variables describing the lightest modalities. We used these directions to compute a correlation matrix between colour characters in order to explore the potential integration and modularity of the melanisation variation. We tested for melanisation integration in organisms by comparing correlations between characters. If melanisation is an integrated process over the entire body, correlation between “light” variables of the different body parts should be mostly positive.
Comparison of colour, genetic and geographic diversity
In order to test the congruence of colour pattern variation with genetic and geographic variation, the distances between colour patterns were compared to the geographic, climatic and genetic distances using pairwise vectors correlation (RV) tests on principal coordinates. The RV coefficient is a multivariate equivalent of the correlation coefficient and addresses the relationship between two sets of variables drawn from a same sample , . Its significance was tested using an approximation of a permutation distribution with the library “FactoMineR” in R , . The different distances were simultaneously available for 84 specimens from eight populations because the genetic sampling comprised fragmented specimens or specimens from the queen caste. Both individual and population distances were analysed. Geographic distances were computed as geodesic distances between GPS coordinates of the different sample localities using the library “oce” . Genetic distances were computed as allele-shared distances and haplotypic distances. Climatic distances were computed on scores of a Principal Component Analysis of eight climatic variables used in a previous niche modelling of the species : Annual mean temperature, Temperature seasonality, Maximum and Minimum annual temperatures, Annual precipitation, Maximum and Minimum monthly precipitations and Precipitation seasonality. These variables were extracted from the BIOCLIM database as five arc-minutes grids (http://www.worldclim.org/; ) on the basis of GPS coordinates, and scaled by their standard deviation. Colour pattern variation was described from scores of the CA. Principal coordinates requiring Euclidean distances, genetic and geodesic distances were transformed following Lingoes . Disparity between the distance matrices was visualised using neighbour joining on the dissimilarity matrix with one minus the squared RV coefficient as a dissimilarity index. We illustrated details of the dissimilarity between haplotypic and phenotypic data by plotting the correlation matrix of distances per individuals between the two datasets and their corresponding hierarchical clustering with the “gplots” package . Under the hypothesis of a strong similarity, the structure of the two clustering trees should be equivalent and the correlation of distances for each individual should approach one. Consequently, the correlation matrix of distances should present clear blocks of high and low correlations related to the corresponding clusters.
The low intra-population diversity produced a similar structure in every distance matrix computed on individual distances. In order to test the correlation of phenotypic, geographic and genetic distances among populations, we also computed distances between populations' colour, climatic, geodesic and genetic averages. We used respectively population mean colour and climatic scores, mean GPS coordinates and mean DAS and haplotype distances.
Previous studies used Fst and Qst estimates to compare phenotypic and neutral genetic differentiation between populations and to test for geographic clines (e.g. , ). However, these estimates involve the intra-population variation  which can hardly be estimated for lowly sampled populations such as some of ours. Furthermore, Qst computation requires an assessment of the heritability and of the additive genetic variance of the phenotype , . These values were unknown for the wing shape of social wasps and a sensitivity analysis showed that they critically influenced our Qst estimates. Therefore, we chose not to use these estimates in our study.
We found 25 different haplotypes in our mitochondrial DNA sampling of V. velutina. Populations were separated into two main clusters: a cluster from the Indonesian archipelago and a cluster from mainland populations (Fig. 5A). More than 15 mutations occurred between these two clusters, while the maximum of divergence within each cluster reaches 11 and 12 mutations for mainland and Indonesian haplotypes respectively (Fig. 5B, C).
A - ML tree computed on CO1 sequences. Scale bar represents the expected mutation per site, node values are bootstrap supports. B - Haplotype network. White diamonds are the inferred mutations. C - Populations sampled. Size of triangles (A) and circles (C) are proportional to the related number of specimens having these haplotypes.
In the ML tree Indonesian haplotypes were grouped in the cluster that was the sister group of all other Asian haplotypes. They were more similar to Nepalese haplotypes than to haplotypes from other geographically closer populations. Indonesian haplotypes exhibited high genetic diversity within and between populations without shared haplotypes between islands.
Haplotypes from mainland Asia were split in two groups not entirely congruent with the geography of populations. Nepalese haplotypes were basal or grouped with haplotypes from the southern part of Vietnam but this group was not supported by bootstrap values. Thai and Yunnan haplotypes were related to Zhejiang haplotypes in a relatively well supported group.
Allele-shared distances were congruent with distance computed with CO1 in distinguishing mainland from Indonesian specimens in the NJ tree. Overall, mainland specimens were split between a western cluster with the specimens from Nepal, Thailand and Vietnam, and an eastern cluster with specimens from Zhejiang. Specimens from Yunnan formed three different groups spread over these two clusters.
Nepal and Zhejiang populations displayed on average similar genetic diversity (θ = 2.29 and 2.55 respectively) with similar number of localities sampled, but the highest genetic diversity was found in the Yunnan population (θ = 2.64).
We found a significant departure from Hardy-Weinberg Equilibrium for many loci of the Yunnan population and for the L2015 and the R4-114 loci of the Nepal population. We thus excluded them from the Bayesian clustering.
Bayesian clustering distinguished Indonesian and continental populations from K = 2 (Fig. 6). According to Evanno's method, K = 4 would be the best estimate number of clusters in the sampling , . At K = 4, continental specimens were split across three clusters, one with Nepal and Thai specimens, a second with Zhejiang specimens and a third with those from Vietnam. With increasing number of K, new clusters occurred mostly within the Zhejiang and Nepal clusters. These relationships may be obscured by the higher allelic diversity found in the Zhejiang and Nepal samples. With K greater than 7, new clusters were not congruent among iterations of simulations. The Indonesian cluster remained as a whole in most iteration, but Sulawesi specimens were found separated from other Indonesian populations in few iterations from K = 5.
The CA returned a colour space with 23 dimensions of which the first dimension gathered 40.64% of the total variation. This axis described a variation from dark to light characters states (Fig. 7). All the vectors of the lightest modalities were on the positive part of the axis. The second dimension encompassed 13.05% of the variation and essentially opposed the coloration of the metanotum and anterior metasomal dorsum to that of the head, legs and pronotum. The third component, accounting for 9.23% of the variation, opposed the coloration of antennal scape and mesepisternum to the coloration of ventral and apico-dorsal metasomal surfaces and the presence of a spot on hind-tibia. The fourth to seventh components mostly described the variation of two characters in relation to all the others: the spot at the apex of the hind-tibia and the spot on the mesepisternum.
Two first dimensions of the colour space resulting from a correspondence analysis on melanisation described by two binary variables of the extreme light and dark coloration for each body part. Intermediate modalities were integrated using a fuzzy coding. Coloured spots described the mean values of each colour morphs (see Fig. 1). Colour forms: N = nigrithorax. K = karnyi. C = celebensis. V = velutina. U = flavitarsus. W = variana. T = timorensis. R = ardens. A = auraria. F = floresiana. D = divergens. P = pruthii. B = sumbana.
Cross-validation confirmed that the colour space separated most of the colour morphs: more than 92.05% of specimens were correctly attributed to their respective colour morph. Of the 31 specimens misidentified from their colour coding, six corresponded to bad discrimination between the auraria and pruthii morphs, for which the main divergence concerns leg colours not taken into account in the analysis.
The MCA resulted in a similar distribution of the specimens with the first axis of global melanisation encompassing 20.8% of the variation. This analysis returned a colour-space with higher dimensionality allowing only for a slightly more accurate attribution of the specimens to their respective colour morphs with cross-validation (93.08%). Results of the MCA will thus not be further discussed.
The correlation matrix computed between the vectors of lighter coloration of the different body parts in the colour space defined by the CA showed 96.84% of positive correlations (Fig. 8). The negative correlations opposed primarily the presence of a colour spot at the apex of hind tibia and the darkening of the fourth metasomal tergum to the darkening of the scape, the metanotum, the first three metasomal terga and the fourth metasomal sternum. Correlation between colour patterns revealed two complexes of correlated characters: one including the three head characters, the anterior leg, pronotum, mesoscutum and scutellum and the other the colour patterns of the metanotum and the three first metasomal terga.
Comparison of colour, genetic and geographic diversity
Comparisons using RV test on the individual distances resulted in a significant relatedness between every dataset. The RV coefficient was the highest between climatic and geodesic distances (RV = 0.680) and the lowest between mitochondrial and colour distances (RV = 0.163). Correlation matrix and hierarchical clustering showed low correlations and clear differences in the structure of these haplotypic and phenotypic distances (Fig. 9). Correlation between the two types of distances was low for most of the specimens. Overall the correlation matrix was not highly structured. Clustering using mitochondrial distances grouped individuals per populations except for Sulawesi, Flores and one specimen from Yunnan, and distinctly separated continental and Indonesian populations. On the contrary, phenotypic distances split most populations and mixed distant specimens. The two main colour clusters separated specimens from Zhejiang, Java, Sulawesi and some specimens from Nepal in one group characterized by a dark head and mesosoma, and specimens from Yunnan, Zhejiang, Vietnam, Flores and Lombok populations presenting a lighter head and mesosoma, in a second group.
Correlation matrix between haplotypic distances (rows) and phenotypic distances (columns) with associated dendrograms. Correlation between distances of a same individual are marked with white squares. Under the hypothesis of similarity between the data, the trees should have the same structure and the individual correlations should approach one. Furthermore, high correlations should be organised in well delimited blocks corresponding to clusters. Haplotypic distances were computed on CO1 sequences and phenotypic distances were computed as the Euclidean distance between individuals in the colour space. Correlation coefficients ranged from −0.6 to 0.6. Dendrograms resulted from complete-linkage clusterings and should not be interpreted as evolutionary trees. Order of specimens differs in rows and columns. Populations: L = Lombok; F = Flores; J = Java; N = Nepal; S = Sulawesi; V = Vietnam; Y = Yunnan; Z = Zhejiang.
Population distances returned fewer significant relationships between the different datasets. Mitochondrial, geodesic and climatic distances were still significantly related but these datasets were no more related to the colour distances and to distances based on genotype data (Table 3).
Evolution of Vespa velutina
Our results highlighted two main genetic groups of populations: one from continental Asia and the other from the Indonesian islands. Genetic variation for CO1 within the Indonesian cluster was equivalent to the variation observed within the continental group. However, the structure of the Indonesian variation was not congruent with geography. The low sampling of these islands limited a more detailed interpretation of this structure and may explain the absence of resolution found with the Bayesian clustering on microsatellite markers. Within the continental cluster, mitochondrial and nuclear markers returned distinct results. Sequences of CO1 clustered populations from Nepal and Vietnam while Thailand, Yunnan and Zhejiang specimens were in a different clade (Fig. 5). The microsatellite markers supported a different partition of populations with Nepal and Thailand as a group, Zhejiang and Vietnam being independent populations (Fig. 6). These differences could be explained by the different transmission of mitochondrial and nuclear DNA combined with the unequal sample sizes of populations. Bayesian clustering returned subgroups within the two populations with the largest samples, Nepal and Zhejiang (Fig. 4), while the discrepancies in results between markers involved Vietnam and Thailand populations, both having very low sample diversity. Subgroups found within the continental group should therefore not be considered as relevant biological groups without further genetic data. Both markers confirmed nonetheless that Yunnan sample was genetically heterogeneous, potentially influenced by high migration from the surrounding populations.
The strong genetic difference between continental and Indonesian populations suggested an ancient divergence of these groups of populations. It also raised the question of a potential speciation of southern populations. Two populations from Sumatra and Peninsular Malaysia are located between these continental and southern populations. The absence of molecular data from these two intermediate populations limits our understanding of the isolation of the Indonesian and continental populations. Furthermore, the lack of morphological characters discriminating the two main groups did not support the hypothesis of a long isolation leading to a speciation event.
The presence of a continental cluster confirmed that populations of the auraria colour form belong to the same species as populations of the nigrithorax and variana forms . Populations of the auraria colour form were long considered as a different species on the basis of sympatry with the colour form nigrithorax in north-eastern India, Nepal, Burma, Assam and western China . In fact, these two colour forms may interbreed: they present intermediate coloration forms in a single locality, as in our Nepal sample presenting typical auraria specimens and darker specimens with a coloration somewhat similar to the nigrithorax form as observed in northern Vietnam . Considering the close relationships between Nepalese nigrithorax and auraria specimens, the different populations of nigrithorax, and possibly auraria, observed in Figure 1 are likely convergent evolution in different populations.
Patterns of melanisation
We quantified the variation of melanism across V. velutina distribution by decomposing the global melanisation pattern into a suite of discrete variations of the different body parts. Some of the colour variation between the populations of V. velutina could not be included in this analysis focusing on defined melanic patterns. For example, the differences in light colour that are clearly yellow or reddish in the different populations were not coded and some variation in leg melanisation was too labile or too rare to be taken into account without overweighting these characters. However, the high rate of correct identification of specimens to their colour morph confirmed that a characterisation of melanin patterns of the different sclerites is a good estimate of the colour variation across the species. Furthermore, the use of a fuzzy coding forcing each character variation to follow a linear direction in the colour space minimally affected the results, when compared to a more complex colour space of each variation considered as a discrete binary variable.
This quantification of melanism highlighted that the melanisation of numerous sclerites varies differently in V. velutina (Table 2). Each sclerite tended to have a well defined set of particular and complex melanin patterns across the distribution (Fig. 4). These patterns involved mostly an antero-posterior extension of the black stripes, on the metasoma (Fig. 4.D and E). Other segments like mesoscutum, metanotum or mesepisternum presented well-defined marks of light colours which size may vary (Fig. 4.B). Finally, these patterns seemed restricted to a given sclerite, not extending to different segments.
Genetic bases of melanin production and patterning genes
The genetic control of pigmentation has been widely studied, notably in fruit flies and butterfly wings –. This genetic control occurs at different scales in melanin synthesis: directly on the genes coding for proteins of the synthesis chain, like the yellow gene , ; indirectly on genes coding for proteins altering melanin precursors, like ebony ; or on genes modifying the expression of these two previous groups of genes, like engrailed or Abdomen-B . Patterning expression of these genes has been related to the diffusion of small compounds called “morphogens” from source cells through the tissues during the development –. Morphogen diffusion is tightly related to temperature, but also to body topology: for example, wing veins act as barriers in signal diffusion across the wing , . This may explain why some light or dark spots in hornets do not extend to their neighbouring sclerites through cuticular sutures, such as those on the metanotum or mesepisternum (Fig. 4).
In our analysis, the black and yellow stripes of the different metasomal segments were formed by an apical yellow stripe extending more or less anteriorly and often interrupted by a median band and sometimes lateral spots of melanin (Fig. 4.D). This variation appeared strikingly similar to the patterned activities of three genes Hox, decapentaplegic, wingless and engrailed, regulating the abdominal pigmentation in Drosophila melanogaster – (Fig. 10). In the fruitfly, engrailed was found responsible for the light band on the posterior margin by repressing the expression of the yellow gene, blocking the production of melanin . On another hand, decapentaplegic seems to enhance the formation of the median band of melanin . Interaction between these three genes and the variation of their level of expression could explain the complex patterns of melanisation of the metasomal segments of social wasps . These three genes are probably not the only genes involved in the variation of melanin patterns, but they may be good candidates for identifying genes involved in the formation of patterns in metasoma melanisation.
A. Expression patterns of engrailed (en), wingless (wg) and decapentaplegic (dpp) on an abdominal tergum of Drosophila (modified from , after , ). B. Variation of melanisation of the third metasomal tergum of V. velutina. The blue dotted lines represent the part of the segment covered by the second tergum.
Variation of melanisation between the different segments
The first axis of variation in the colour space described a global variation in melanisation and appeared positively correlated to the 23 variables of light coloration (Fig. 7). Furthermore these variables were positively correlated in 96.84% of the pairwise comparisons. The correlation between vectors of melanisation of each body part was not homogeneous and suggested two modules: one combining the melanisation of the cephalic capsule together with the pronotum and scutellum, and another the melanisation of the three first metasomal terga with metanotum and in a lesser extent with mesoscutum (Fig. 8). Together, these results suggested a partial integration of melanisation variation over the body, with potential regionalisation of this phenotype.
Because the aposematic signal is located over the entire body in social wasps, such integration and modularity may enhance the quality of the warning signal of the different variants. Colour integration over the body may result from variation of the general regulation of coloration. Two regulation processes of the pigmentation are hormonal levels, melanin production being related to ecdysone titre , , and genetic variations. Sensitivity of genes involved in melanin synthesis to morphogens may differ from one population to another: the influence of the different morphogens in inhibiting or stimulating a gene expression results from the ability of their transcriptional regulators to bind to a regulatory region of the DNA next to the coding part of the gene . For the yellow gene, a cis-regulatory promoter has been identified as responsible for pigmentation difference between species due to evolutionary changes altering the number of binding sites of the regulators , . Variation in these cis-regulatory sequences returned different pigmentations of several body parts, either the whole abdomen, sometime with the thorax, or only the two last abdominal segments. The integration of melanism over the body and its structure in modules may thus be linked to variation in hormonal production, but also to a difference in regulatory genes' sensitivity to morphogens' signals that evolutionary changes alter from one population to another.
Colour variation between populations
Our results of comparisons of distance matrices showed that colour pattern diversity did not match the evolutionary history described by our analysis of molecular markers, nor the geographic distances or the climatic similarity between populations (Figs. 9). Climate, geographic and mitochondrial based distances presented similar structure, but the data based on genotypic and phenotypic distances were differently structured. The absence of congruence between genotypic and CO1 sequences or geography may indicate an influence of the restricted population sampling on the genotypic distances. Another hypothesis would be an unbalanced dispersal of males and females in the species, with males dispersing more than females; however this is not congruent with our current knowledge of this species , .
Non congruence of colour-based distances with the other dataset was explained by the presence of several populations having very dark colour patterns both in Indonesia and on the continent, while a southern Indonesian population has the lightest colour morph, similar to the Malaysian population and, in a lesser extent, to the continental eastern populations (Fig. 1). On the other hand, Indonesian and continental populations are two divergent lineages discriminated by both mitochondrial and nuclear markers. Most of the similar patterns of melanisation found between these two lineages are thus convergences with different evolutionary origins.
In theory, such convergence could be either induced by a high phenotypic plasticity or by convergent evolutions of genes regulating the melanisation process. Phenotypic plasticity of the coloration has been shown in butterflies through day-length influence on the hormonal production  and in paper wasps through variation in rearing temperature  and diet . Tibetts highlighted that diet influenced only limited melanin patterns involved in social signal such as the clypeus markings . MacLean and her collaborators showed that colour variation in paper wasps induced by a rearing temperature change did not reach the inter-population variation . Furthermore, diet of a single colony of hornet can change through the season in temperate areas  without inducing a colour variation in individuals (pers. obs.). Finally, in contrast to the paper wasps studied by Green et al. , hornet larvae develop in enclosed nests with buffered temperature and humidity, lowering the influence of external physical factors , . Dark specimens of V. velutina from semi-tropical China have been accidentally introduced to temperate areas of France and Korea several years ago ,  and these invasive populations did not change in coloration , . It is therefore unlikely that the observed convergences in coloration of several V. velutina populations are due to phenotypic plasticity induced by similar local factors.
A more probable hypothesis is a parallelism in the evolution of the regulatory and patterning genes either by similar or dissimilar mutations . This evolution may also have been influenced by extrinsic factors through selection: melanism, with a genetic basis, can have both positive and negative impacts on the organism's fitness , . The different factors that may induce a selection on melanism are related to the climate and associated environments, but also to the local communities of predators and of Müllerian mimics.
Climate is known to have an influence on melanism in insects. Melanism has been related to thermoregulation  and dessication resistance , . Higher melanic insects were found to warm up faster under the sun, to reach higher temperature, but also to lose less water and resist desiccation better than lighter morphs , .
However, studies do not agree on the distribution of melanic forms. Some authors suggest that melanism occurs mostly in dry environments , while other argues that they are found in cool and wet habitats ,  or in tropical areas . Our results concur only partially with this last study. Two of the least melanised populations occur respectively in Nepal and in the driest island of the distribution of V. velutina in Indonesia while geographically intermediate populations are mostly darker, with the exception of the population from Malaysia. However, we found only low structured climatic differences between the localities of light and dark coloured populations and no cline is visible (Fig. 1). Melanism does not appear to be influenced by altitude, average or extreme temperatures or humidity of the locality of sampling. Especially, the difference between the geographically close Malaysian and Sumatran colour forms, respectively the lightest and the darkest, rejects the hypothesis of climate pressures as the major evolutionary force driving colour polymorphism in V. velutina.
Besides physical elements of the environment, hornets are likely to be selected for the efficiency of their warning colours . Like other social wasps, hornets have a painful sting and are distasteful due to their venom gland, making them avoided by predators  , . They benefit from being recognized by potential predators that may attack them or disturb their nests. Furthermore, predator avoidance may be critical in one part of the life-cycle of V. velutina: like in many other social wasps, each colony is annual and founded by one solitary queen . At this time, selective pressures within the species are high, as each queen has to survive for weeks before being able to produce the next generation. As such, warning colours play an important role in social wasps by protecting queens during their solitary phase.
The notion of warning colours depends on the perception of colours by predators , . While dark coloration may be advantageous for crypsis , the yellow coloration seems to be a warning colour naturally avoided by bird predators . Vidal-Cordero and his collaborators also showed a direct link between the lightness of the metasoma pigmentation in a paper wasp and the size of its venom gland . Light coloration may therefore intervene in predator avoidance through its intensity.
On another hand, black and yellow stripes patterns help in prey recognition both by vertebrate ,  and invertebrate predators . This may explain the presence of black and yellow metasomal stripes in most of the V. velutina populations and the potential patterns observed in mesosoma: the pronotum, the scutellum and sometimes the metanotum can become lightly coloured while the mesoscutum and the propodeum always present black markings. This coloration creates over the mesosoma an alternating pattern of light and black colours extending the striped pattern of the metasoma (Fig. 4.B). It is therefore unsurprising to find yellow marks even on the darkest morphs of V. velutina as well as black segments and markings on the lightest populations (Figs. 1 & 4).
The influence of warning signal on predator behaviour depends on other harmful species encountered by the predators through the process of Müllerian mimicry, different harmful species sharing a similar aposematic signal, thus mimicking each other. This mimicry reinforces the impact of warning coloration by augmenting the probability of association between the bad experience of the predator and the signal displayed . This phenomenon has been widely studied in butterflies , , but is also known among bumblebee species , ,  and wasps . Both being stinging Hymenoptera, bumblebees, bees and wasps are likely to be recognized as similar prey items by predators and may share the Müllerian effect of their coloration. For example, Hines and Williams pointed out drastic variation of colour in bumblebees from Malaysia, for which specimens are fully orange , matching the singular orange morph divergens of V. velutina found in the same region. Another example is the mimicry between the nigrithorax form of V. velutina and queens of two yellow jacket species Vespula koreensis (Radoszkowski, 1887) and Vespula orbata (du Buysson, 1902) found in southern China.
Presence of Müllerian mimicry is also often accompanied by strong phenotypic variation structured in mosaic across the distribution of the species . Polymorphism between populations of V. velutina may thus be the result of different selective pressures induced both by variation in the local stinging Hymenoptera communities and by the pressures on melanism-related traits: crypsis, thermoregulation, desiccation and pathogen resistance. While hornets may not be highly sensitive to some of these pressures, co-mimic species with open nests or solitary habits may be, thus leading to an indirect effect of these factors on hornet coloration. Furthermore, the critical phase in colony development associated with the potential founder effects in island colonisation during Vespa velutina evolutionary history may explain the high variety of coloration found across islands while colour variation is more progressive and colour forms more widespread in continental Asia.
In order to test for this hypothesis, further studies could focus on characterising the melanism of the different species of stinging Hymenoptera of similar sizes in different regions. This work has already been started on bumblebees , ,  but should be extended over the different families of Hymenoptera, as mimetism is not restrained taxonomically.
Our study described the changes in patterns of melanisation over the distribution of the hornet species Vespa velutina. The main axis of pattern variation in the resulting colour space described a global melanisation of the body indicating that the melanisation is a partially integrated process across the distribution. Correlations among the melanisation of the different body parts revealed a structured variation with two apparent modules: one composed of the head capsule, anterior part of mesosoma and anterior legs and the other connecting melanisation of the anterior metasomal dorsum and metanotum.
Based on mitochondrial sequences and microsatellite markers, we identified two highly divergent lineages within the V. velutina species: a continental lineage and a lineage restricted to southern Indonesian islands. The existence of a continental lineage confirmed the synonymy of V. auraria under V. velutina. The evolutionary history of the species could not explain the observed colour variations: the variation in melanisation clearly included convergence in different populations. Comparison of the climatic, genetic, geographic and colour diversities showed that melanism was unlikely driven by abiotic factors such as climate variation or latitudinal clines. This variation may result instead of mutations selected by high local constrains on aposematism and Müllerian mimicry with potential influence of the founder effects in islands.
Together, these results confirmed that colour patterns should not be regarded as reliable criteria for population relationships or species distinction in social wasps. They also suggest that colour variation in aposematic species is not tightly related to abiotic conditions. Further studies could use the quantification of colour patterns to track correlated changes between species within communities of aposematic species and test for the influence of Müllerian mimicry, predators and habitat types on the geographic variation of aposematic coloration.
List of light colour characters with their modalities. All modalities are depicted in Figure 4. The coding for the different modalities is mentioned in brackets for the 23 light coloration characters. The coding for the 23 dark characters is equivalent to one minus the corresponding light coloration character.
We thank Adrien Quiles and Claire Capdevielle Dulac for their help with microsatellite analysis, and Nelly Gidaszewski, Mathieu Joron, Alexandre Roulin and the two anonymous reviewers for their helpful comments on the subject and the manuscript. We are grateful to Alain Roques (INRA, Orléans), Agnès Rortais (CNRS, Gif-sur-Yvette), but also the LIPI authorities from Indonesia, especially our counterparts Yayuk Suhardjono and Oscar Effendy, NARC from Nepal, Truong Quang Tam (Institute of Tropical Biology, Vietnam) and the Pr. Xue-xin Chen (University of Zhejiang, China) for helping us to collect specimens in Asia. We are also grateful to Josef Gusenleitner, Jean Haxaire, Jean-Luc Renesson and Pierre Tripotin that shared their private collections, and Rémi Perrard for his help with posterior GPS coordinates of ancient sampling localities. Special thanks are due to curators Alain Drumont, Yvonnick Gérard, Kees van Achterberg, Fritz Gusenleitner and Gavin Broad who allowed the study in their respective museum collections. This study benefited of other public collections: the American Museum of Natural History (New York, USA), the University of Zhejiang (Hangzhou, China), the Museum Zoologicum Bogoriense (Bogor, Indonesia) and the Muséum National d'Histoire Naturelle (Paris, France).
Conceived and designed the experiments: AP MA JFS CV. Performed the experiments: AP MA QR FM JT SB HN CV. Analyzed the data: AP MA RB MB CV. Contributed reagents/materials/analysis tools: AP MA QR FM JT SB HN JFS JMC CV. Wrote the paper: AP MA MB JFS JMC CV.
- 1. Poulton EB (1890) The colours of animals: their meaning and use, especially considered in the case of insects. D. Appleton. 360 p.
- 2. Gehara M, Summers K, Brown JL (2013) Population expansion, isolation and selection: novel insights on the evolution of color diversity in the strawberry poison frog. Evol Ecol 27: 797–824.
- 3. Lehtonen PK, Laaksonen T, Artemyev AV, Belskii E, Both C, et al. (2009) Geographic patterns of genetic differentiation and plumage colour variation are different in the pied flycatcher (Ficedula hypoleuca). Mol Ecol 18: 4463–4476.
- 4. Mallet J, Joron M (1999) Evolution of diversity in warning color and mimicry: polymorphisms, shifting balance, and speciation. Annu Rev Ecol Syst 30: 201–233.
- 5. Ruxton GD, Sherratt TN, Speed MP (2004) Avoiding attack: the evolutionary ecology of crypsis, warning signals, and mimicry. Oxford University Press Oxford. 249 p.
- 6. Stevens M, Ruxton GD (2012) Linking the evolution and form of warning coloration in nature. Proc R Soc Lond B Biol Sci 279: 417–426.
- 7. Wallace AR (1878) Tropical Nature and Other Essays. Kessinger Publishing, LLC. 356 p.
- 8. Kauppinen J, Mappes J (2003) Why are wasps so intimidating: field experiments on hunting dragonflies (Odonata: Aeshna grandis). Anim Behav 66: 505–511.
- 9. Hauglund K, Hagen SB, Lampe HM (2006) Responses of domestic chicks (Gallus gallus domesticus) to multimodal aposematic signals. Behav Ecol 17: 392–398.
- 10. Yachi S, Higashi M (1998) The evolution of warning signals. Nature 394: 882–884.
- 11. Speed MP, Ruxton GD (2005) Aposematism: what should our starting point be? Proc R Soc Lond B Biol Sci 272: 431–438.
- 12. Joron M, Jiggins CD, Papanicolaou A, McMillan WO (2006) Heliconius wing patterns: an evo-devo model for understanding phenotypic diversity. Heredity 97: 157–167.
- 13. Wang IJ, Shaffer HB (2008) Rapid color evolution in an aposematic species: a phylogenetic analysis of color variation in the strikingly polymorphic strawberry Poison-Dart Frog. Evolution: 2742–2759.
- 14. Archer ME (1991) The number of species that can be recognized within the genus Vespa (Hym., Vespinae). Entomol Month Mag 127: 161–164.
- 15. van der Vecht J (1957) The Vespinae of the Indo-Malayan and Papuan areas (Hymenoptera, Vespidae). Zool Verhandel 34: 1–82.
- 16. MacLean B K, Chandler L, MacLean DB (1978) Phenotypic expression in the paper wasp Polistes fuscatus (Hymenoptera: Vespidae). Great Lakes Entomol 11: 105–116.
- 17. Nappi AJ, Vass E (1993) Melanogenesis and the generation of cytotoxic molecules during insect cellular immune reactions. Pigment Cell Res 6: 117–126.
- 18. Wilson K, Cotter SC, Reeson AF, Pell JK (2001) Melanism and disease resistance in insects. Ecol Lett 4: 637–649.
- 19. True JR (2003) Insect melanism: the molecules matter. Trends Ecol Evol 18: 640–647.
- 20. Roff DA, Fairbairn DJ (2013) The costs of being dark: the genetic basis of melanism and its association with fitness-related traits in the sand cricket. J Evol Biol 26: 1406–1416.
- 21. Lindstedt C, Lindström L, Mappes J (2009) Thermoregulation constrains effective warning signal expression. Evolution 63: 469–478.
- 22. du Buysson R (1905) Monographie des guêpes ou Vespa. Ann Soc Entomol Fr 72: 260–288.
- 23. Bequaert J (1930) On the generic and subgeneric divisions of the Vespinae (Hymenoptera). Bull Brooklyn Entomol Soc 25: 59–70.
- 24. Olson EC, Miller RI (1958) Morphological Integration. University of Chicago Press, Chicago, USA. 355 p.
- 25. Klingenberg CP (2013) Cranial integration and modularity: insights into evolution and development from morphometric data. Hystrix 24: 43–58.
- 26. Klingenberg CP (2008) Morphological integration and developmental modularity. Annu Rev Ecol Evol Syst 39: 115–132.
- 27. Antoniazza S, Burri R, Fumagalli L, Goudet J, Roulin A (2010) Local adaptation maintains clinal variation in melanin-based coloration of European Barn Owls (Tyto alba). Evolution 64: 1944–1954.
- 28. Sherratt TN (2008) The evolution of Müllerian mimicry. Naturwissenschaften 95: 681–695.
- 29. Archer ME (1994) Taxonomy, distribution and nesting biology of the Vespa bicolor group (Hym., Vespinae). Entomol Month Mag 130: 149–158.
- 30. Van der Vecht J (1959) Notes on Oriental Vespinae, including some species from China and Japan (Hymenoptera, Vespidae). Zool Mededel 13: 205–232.
- 31. Nguyen LTP, Saito F, Kojima J, Carpenter JM (2006) Vespidae of Viet Nam (Insecta: Hymenoptera) 2. Taxonomic Notes on Vespinae. Zoolog Sci 23: 95–104.
- 32. Perrard A, Pickett K, Villemant C, Kojima J, Carpenter J (2013) Phylogeny of hornets: a total evidence approach (Hymenoptera, Vespidae, Vespinae, Vespa). J Hym Res 32: 1–15.
- 33. Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol 3: 294–299.
- 34. Hajibabaei M, Ivanova NV, Ratnasingham S, Dooh RT, Kirk SL, et al. (2005) Critical factors for assembling a high volume of DNA barcodes. Philos Trans R Soc Lond B Biol Sci 360: 1959–1967.
- 35. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41: 95–98.
- 36. Arca M, Capdevielle-Dulac C, Villemant C, Mougel F, Arnold G, et al. (2011) Development of microsatellite markers for the yellow-legged Asian hornet, Vespa velutina, a major threat for European bees. Conserv Genet Resour 4: 1–4.
- 37. Daly D, Archer ME, Watts PC, Speed MP, Hughes MR, et al. (2002) Polymorphic microsatellite loci for eusocial wasps (Hymenoptera: Vespidae). Mol Ecol Notes 2: 273–275.
- 38. Arca M (2012) Caractérisation génétique et étude comportementale d'une espèce envahissante en France: Vespa velutina Lepeletier (Hymenoptera, Vespidae) Doctoral Thesis, Paris: Université Pierre et Marie Curie. 199p.
- 39. Bandelt HJ, Forster P, Röhl A (1999) Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol 16: 37–48.
- 40. Hordijk W, Gascuel O (2005) Improving the efficiency of SPR moves in phylogenetic tree search methods based on maximum likelihood. Bioinformatics 21: 4338–4347.
- 41. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4: 406–425.
- 42. Chakraborty R, Jin L (1993) A unified approach to study hypervariable polymorphisms: statistical considerations of determining relatedness and population distances. In: Pena S, Jeffreys A, Epplen J, Chakraborty R, editors. DNA fingerprinting, current state of the science.Basel: Birkhauser. pp. 153–175.
- 43. Paradis E, Claude J, Strimmer K (2004) Ape: Analyses of phylogenetics and evolution in R language. Bioinformatics 20: 289–290.
- 44. Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155: 945–959.
- 45. Excoffier L, Laval G, Schneider S (2005) Arlequin (version 3.0): an integrated software package for population genetics data analysis. Evol Bioinform Online 1: 47–50.
- 46. Chevene F, Doléadec S, Chessel D (1994) A fuzzy coding approach for the analysis of long-term ecological data. Freshw Biol 31: 295–309.
- 47. Escoufier Y (1973) Le traitement des variables vectorielles. Biometrics 29: 751–760.
- 48. Robert P, Escoufier Y (1976) A unifying tool for linear multivariate statistical methods: The RV Coefficient. J R Stat Soc C Appl Stat 25: 257–265.
- 49. Josse J, Pagès J, Husson F (2008) Testing the significance of the RV coefficient. Comput Stat Data An 53: 82–91.
- 50. Husson F, Josse J, Lê S, Mazet J (2013) FactoMineR: Multivariate Exploratory Data Analysis and Data Mining with R, R package version 1.24. CRAN website. Available: http://CRAN.R-project.org/package=FactoMineR. Accessed 2013 September 18.
- 51. Kelley D (2009) oce: Analysis of Oceanographic data. R package version 0.1–80. CRAN website. Available: http://CRAN.R-project.org/package=oce. Accessed 2013 September 18.
- 52. Villemant C, Barbet-Massin M, Perrard A, Muller F, Gargominy O, et al. (2011) Predicting the invasion risk by the alien bee-hawking Yellow-legged hornet Vespa velutina nigrithorax across Europe and other continents with niche models. Biol Cons 144: 2142–2150.
- 53. Hijmans RJ, Cameron SE, Parra JL, Jones PG, Jarvis A (2005) Very high resolution interpolated climate surfaces for global land areas. Int J Climatol 25: 1965–1978.
- 54. Lingoes JC (1971) Some boundary conditions for a monotone analysis of symmetric matrices. Psychometrika 36: 195–203.
- 55. Warnes GR, Bolker B, Bonebakker L, Gentleman R, Huber W, et al. (2013) gplots: Various R programming tools for plotting data. R package version 2 113. CRAN website. Available: http://CRAN.R-project.org/package=gplots. Accessed 2013 September 18.
- 56. Chapuis E, Martin G, Goudet J (2008) Multivariate Qst–Fst comparisons: a neutrality test for the evolution of the G matrix in structured populations. Genetics 180: 2135–2149.
- 57. Leinonen T, McCairns RS, O'Hara RB, Merilä J (2013) Qst–Fst comparisons: evolutionary and ecological insights from genomic heterogeneity. Nat Rev Gen 14: 179–190.
- 58. Brommer JE (2011) Whither PST? The approximation of Qst by Pst in evolutionary and conservation biology. J Evol Biol 24: 1160–1168.
- 59. Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol 14: 2611–2620.
- 60. Waples RS, Gaggiotti O (2006) INVITED REVIEW: What is a population? An empirical evaluation of some genetic methods for identifying the number of gene pools and their degree of connectivity. Mol Ecol 15: 1419–1439.
- 61. Walter MF, Black BC, Afshar G, Kermabon A-Y, Wright TR, et al. (1991) Temporal and spatial expression of the yellow gene in correlation with cuticle formation and DOPA decarboxylase activity in Drosophila development. Dev Biol 147: 32–45.
- 62. Wittkopp PJ, True JR, Carroll SB (2002) Reciprocal functions of the Drosophila yellow and ebony proteins in the development and evolution of pigment patterns. Development 129: 1849–1858.
- 63. Wittkopp PJ, Beldade P (2009) Development and evolution of insect pigmentation: genetic mechanisms and the potential consequences of pleiotropy. Semin Cell Dev Biol 20: 65–71.
- 64. Joron M, Frezal L, Jones RT, Chamberlain NL, Lee SF, et al. (2011) Chromosomal rearrangements maintain a polymorphic supergene controlling butterfly mimicry. Nature 477: 203–206.
- 65. Koch PB, Lorenz U, Brakefield PM (2000) Butterfly wing pattern mutants: developmental heterochrony and co-ordinately regulated phenotypes. Dev Genes Evol 210: 536–544.
- 66. Jeong S, Rokas A, Carroll SB (2006) Regulation of body pigmentation by the Abdominal-B Hox protein and its gain and loss in Drosophila evolution. Cell 125: 1387.
- 67. French V, Brakefield PM (1992) The development of eyespot patterns on butterfly wings: morphogen sources or sinks? Development 116: 103–109.
- 68. French V, Brakefield PM (1995) Eyespot development on butterfly wings: the focal signal. Dev Biol 168: 112–123.
- 69. Murray JD (2003) Mathematical Biology II: Spatial Models and Biomedical Applications. 2003. Springer-Verlag, New York. 814 p.
- 70. Koch PB, Nijhout HF (2002) The role of wing veins in colour pattern development in the butterfly Papilio xuthus (Lepidoptera: Papilionidae). Europ J Entomol 99: 67–72.
- 71. Reed RD, Gilbert LE (2004) Wing venation and Distal-less expression in Heliconius butterfly wing pattern development. Dev Genes Evol 214: 628–634.
- 72. Kopp A, Blackman RK, Duncan I (1999) Wingless, decapentaplegic and EGF receptor signaling pathways interact to specify dorso-ventral pattern in the adult abdomen of Drosophila. Development 126: 3495–3507.
- 73. Kopp A, Duncan I, Carroll SB (2000) Genetic control and evolution of sexually dimorphic characters in Drosophila. Nature 408: 553–559.
- 74. Kopp A, Duncan I (2002) Anteroposterior patterning in adult abdominal segments of Drosophila. Dev Biol 242: 15–30.
- 75. Nijhout HF (2010) Molecular and physiological basis of colour pattern formation. Adv In Insect Phys 38: 219–265.
- 76. Koch BP, Merk R, Reinhardt R, Weber P (2003) Localization of ecdysone receptor protein during colour pattern formation in wings of the butterfly Precis coenia (Lepidoptera: Nymphalidae) and co-expression with Distal-less protein. Dev Genes Evol 212: 571–584.
- 77. Wray GA (2007) The evolutionary significance of cis-regulatory mutations. Nat Rev Genet 8: 206–216.
- 78. Gompel N, Prud'homme B, Wittkopp PJ, Kassner VA, Carroll SB (2005) Chance caught on the wing: cis-regulatory evolution and the origin of pigment patterns in Drosophila. Nature 433: 481–487.
- 79. Matsuura M, Yamane S (1990)Biology of the vespine wasps. Springer Verlag. 323 p.
- 80. Nijhout HF (2003) Development and evolution of adaptive polyphenisms. Evol Dev 5: 9–18.
- 81. Green JP, Rose C, Field J (2012) The role of climatic factors in the expression of an intrasexual signal in the paper wasp Polistes dominulus. Ethology 118: 766–774.
- 82. Tibbetts EA (2010) The condition dependence and heritability of signaling and nonsignaling color traits in paper wasps. Am Nat 175: 495–503.
- 83. Riabinin K, Kozhevnikov M, Ishay JS (2004) Ventilating activity at the hornet nest entrance. J Ethol 22: 49–53.
- 84. Jones JC, Oldroyd BP (2006) Nest thermoregulation in social insects. Adv In Insect Phys 33: 153–191.
- 85. Villemant C, Haxaire J, Streito JC (2006) Premier bilan de l'invasion de Vespa velutina Lepeletier en France (Hymenoptera, Vespidae). Bull Soc Entomol Fr 111: 535–538.
- 86. Perrard A, Muller F, Rome Q, Villemant C (2011) Observations sur le Frelon asiatique à pattes jaunes, Vespa velutina Lepeletier, 1836 (Hymenoptera, Vespidae). Bull Soc Entomol Fr 116: 159–164.
- 87. Wittkopp PJ, Williams BL, Selegue JE, Carroll SB (2003) Drosophila pigmentation evolution: divergent genotypes underlying convergent phenotypes. Proc Natl Acad Sci 100: 1808–1813.
- 88. Pereboom JJM, Biesmeijer JC (2003) Thermal constraints for stingless bee foragers: the importance of body size and coloration. Oecologia 137: 42–50.
- 89. Parkash R, Singh S, Ramniwas S (2009) Seasonal changes in humidity level in the tropics impact body color polymorphism and desiccation resistance in Drosophila jambulina—Evidence for melanism-desiccation hypothesis. J Insect Physiol 55: 358–368.
- 90. Parkash R, Chahal J, Sharma V, Dev K (2012) Adaptive associations between total body color dimorphism and climatic stress-related traits in a stenothermal circumtropical Drosophila species. Insect Sci 19: 247–262.
- 91. Williams P (2007) The distribution of bumblebee colour patterns worldwide: possible significance for thermoregulation, crypsis, and warning mimicry. Biol J Linn Soc Lond 92: 97–118.
- 92. Mostler G (1935) Beobachtungen zur Frage der Wespenmimikry. Z Morphol Oekol Tiere 29: 381–454.
- 93. Gilbert F (2005) The Evolution of Imperfect Mimicry. In: Fellowes M, Holloway G, Rolff J, editors. Insect evolutionary ecology. Wallingford: CABI Publishing. pp. 231–288.
- 94. Théry M, Gomez D (2010) Insect colours and visual appearance in the eyes of their predators. Adv In Insect Phys 38: 267–353.
- 95. Stelzer RJ, Raine NE, Schmitt KD, Chittka L (2010) Effects of aposematic coloration on predation risk in bumblebees? A comparison between differently coloured populations, with consideration of the ultraviolet. J Zool 282: 75–83.
- 96. Vidal-Cordero JM, Moreno-Rueda G, López-Orta A, Marfil-Daza C, Ros-Santaella JL, et al. (2012) Brighter-colored paper wasps (Polistes dominula) have larger poison glands. Front Zool 9: 1–5.
- 97. Schuler W, Hesse E (1985) On the function of warning coloration: a black and yellow pattern inhibits prey-attack by naive domestic chicks. Behav Ecol Sociobiol 16: 249–255.
- 98. Joron M, Wynne IR, Lamas G, Mallet J (1999) Variable selection and the coexistence of multiple mimetic forms of the butterfly Heliconius numata. Evol Ecol 13: 721–754.
- 99. Plowright RC, Owen RE (1980) The evolutionary significance of bumble bee color patterns: a mimetic interpretation. Evolution 34: 622–637.
- 100. Hines HM, Williams PH (2012) Mimetic colour pattern evolution in the highly polymorphic Bombus trifasciatus (Hymenoptera: Apidae) species complex and its comimics. Zool J Linn Soc 166: 805–826.
- 101. O'Donnell S, Joyce F J (1999) Dual mimicry in the dimorphic eusocial wasp Mischocyttarus mastigophorus Richards (Hymenoptera: Vespidae). Biol J Linn Soc Lond 66: 501–514.