Mice were transplanted DL cells (1x105cells/0.5 ml PBS) on day 0 following administration of Vehicle alone (control) or containing orlistat 240mg/kg body weight/day up to day 14 post tumor transplantation. On day 16 BMC were harvested from femurs.
BMDM differentiated from BMC of control or orlistat-administered tumor-bearing mice were incubated in vitro for 24h in medium alone or containing IFN- γ (100IU/ml) + LPS (10ng/ml) followed by estimation of NO (a), indicated cytokines by ELISA in culture supernatant (b), assay of ROS expression (d,e), phagocytosis (c), BMDM-mediated tumoricidal activity (f,g) and expression of cell surface functional markers: CD11c and TLR2 (h). Values shown in (a,b,e,f,g) mean ± SD of three independent experiments done in triplicate.*p<0.05 vs. values of respective control. *#p<0.05 vs. values for orlistat and LPS + IFN-γ treated control groups. Arrows indicates increased phagocytosis (c), expression of ROS (d) and CD11c & TLR-2 (h) in BMDM of orlistat group treated with IFN-γ + LPS.
Figure 1 is incorrect. The authors have provided a corrected version here.
Figure 6 is incorrect. The authors have provided a corrected version here.
Citation: The PLOS ONE Staff (2014) Correction: Myelopoietic Efficacy of Orlistat in Murine Hosts Bearing T Cell Lymphoma: Implication in Macrophage Differentiation and Activation. PLoS ONE 9(3): e93312. https://doi.org/10.1371/journal.pone.0093312
Published: March 24, 2014
Copyright: © 2014 The PLOS ONE Staff. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.