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Nasal Carriage of Staphylococcus aureus in Botucatu, Brazil: A Population-Based Survey

Nasal Carriage of Staphylococcus aureus in Botucatu, Brazil: A Population-Based Survey

  • Fabiana Venegas Pires, 
  • Maria de Lourdes Ribeiro de Souza da Cunha, 
  • Lígia Maria Abraão, 
  • Patrícia Y. F. Martins, 
  • Carlos Henrique Camargo, 
  • Carlos Magno Castelo Branco Fortaleza
PLOS
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Abstract

Recent increases in the incidence and severity of staphylococcal infections renewed interest in studies that assess the burden of asymptomatic carriage of Staphylococcus aureus in the community setting. We conducted a population-based survey in the city of Botucatu, Brazil (122,000 inhabitants), in order to identify the prevalence of nasal carriage of Staphylococcus aureus (including methicillin-resistant strains). Nasal swabs were obtained from 686 persons over one year of age. Resistance to methicillin was assessed through phenotypic methods, identification of the mecA gene and typing of the Staphylococcal Chromosome Cassette mec (SCCmec). Methicillin-resistant S. aureus (MRSA) isolates were characterized using Pulsed-Field Gel Electrophoresis (PFGE), Multilocus Sequence Typing (MLST) and spa typing. Polymerase chain reaction was applied to identify genes coding for Panton-Valentine Leukocidin (PVL) in isolates. The prevalence of overall S. aureus carriage was 32.7% (95%CI, 29.2%–36.2%). Carriers were significantly younger (mean age, 28.1 versus 36.3 for non-carriers; OR for age, 0.98; 95%CI, 0.97–0.99) and likely to report recent skin infection (OR, 1.85; 95%CI, 1.03–3.34). Carriage of methicillin-resistant S. aureus (MRSA) was found in 0.9% of study subjects (95%CI, 0.4%–1.8%). All MRSA isolates harbored SCCmec type IV, and belonged to spa types t002 or t021, but none among them harbored genes coding for PLV. In MLST, most isolates belonged to clones ST5 or ST1776. However, we found one subject who carried a novel clone, ST2594. Two out of six MRSA carriers had household contacts colonized with isolates similar to theirs. Our study pointed to dissemination of community-associated MRSA among the Brazilian population.

Introduction

Recent reports point out to an increase in incidence and severity of staphylococcal infections arising in the community [1], [2]. The emergence and worldwide spread of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) worsened this picture [3]. Most CA-MRSA strains are genetically unrelated to hospital-acquired clones. Also, they have been implicated in the etiology of severe diseases, such as necrotizing pneumonia and skin and soft tissue infection. The severity of CA-MRSA infections has been linked to a major virulence factor, the Panton-Valentine Leukocidin (PVL) [4].

This phenomenon renewed interest in research focusing on the epidemiology of S. aureus as a whole and MRSA, both in the community and in healthcare settings. Some studies were directed towards identifying the burden of MRSA infections. Klevens et al. reported that, among 8,987 invasive MRSA infections from 9 sites in the United States (U.S.), 13.7% could be classified as community-associated [5]. From a different perspective, studies from Europe reported MRSA accounting for 6% to 30% of S. aureus infections in outpatients [6]. In Asian countries that proportion ranged from 2.5% to 39% [7]. One study conducted in five major African towns, including 542 patients with S. aureus infections, identified 86 MRSA isolates, of which 9 were classified as CA-MRSA [8]. Those cases accounted for 11.5% of community-associated S. aureus infections, though data may be biased due to criteria for collecting cultures [9].

There is strong evidence of continuous increase in the incidence of CA-MRSA disease during the past decade, at least in the U.S. [10]. Even though data from Europe suggest that the threat posed by CA-MRSA is lower when compared to the U.S., cases of invasive infections are regularly reported in several countries [6], [11], [12]. Reports of CA-MRSA infections in Africa [8] and Asia [7], [13] confirm the worldwide importance of this pathogen.

In order to identify the sources and dynamics of CA-MRSA in the general population, some epidemiological studies addressed the prevalence and determinants of asymptomatic carriage (also termed “colonization”) of S. aureus and MRSA in the community setting. Those studies are based on the evidence that colonization represents a reservoir for pathogenic strains and/or a pre-infectious stage [14], [15]. In the U.S., the nasal carriage of S. aureus was assessed in 9,622 persons as part of the National Health and Nutrition Examination Survey (NHANES) in 2001–2002. The study found prevalence of 32.4% and 0.8% for S. aureus and MRSA, respectively [16], [17]. In the following years, there was a slight decrease in the overall prevalence of S. aureus colonization, while the carriage of MRSA almost doubled [18].

Even though extensive research has focused on the global epidemiology of S. aureus and MRSA, there are significant gaps of information for regions in Asia, Africa and South America [19]. In Brazil, reports of CA-MRSA infections are scarce, and all data and strains were obtained from patients in university hospitals from highly populated cities [20][22].

Our study was designed to fill this gap, providing population-based data about S. aureus and MRSA colonization in a small city in inner Brazil. We also aimed to identify factors associated to colonization and the molecular epidemiology of colonizing strains.

Materials and Methods

Ethical issues

This study was conducted according to the principles expressed in the Declaration of Helsinki. It was approved by the reference Committee for Ethics in Research (“Comitê de Ética em Pesquisa” from “Faculdade de Medicina de Botucatu”. City of Botucatu, São Paulo State, Brazil). A written informed consent was obtained from all study subjects or their legal guardians.

Study design and population

We conducted a cross-sectional study in order to identify the prevalence of carriage S. aureus among urban population in the city of Botucatu, Brazil (22°53′09″S, 48°26′42″W). The city is located in São Paulo State, in Southeastern Brazil. It has approximately 122,000 inhabitants, 95% of whom live in urban areas. The survey was conducted during the year 2011.

The sample size was estimated in OpenEpi software (Emory University, Atlanta, GA) using the following parameters: city population, 122,000; anticipated S. aureus prevalence, 32.4%; confidence limits, 5%; design effect, 2. We estimated the inclusion of 672 subjects. The sample size was later extended for 686, for operational reasons.

The sample was stratified for age and gender, according to the 2010 census data. Subjects with less than one year of age were excluded. Households and subjects from the urban area of Botucatu were selected from an updated version of the database used in the São Paulo State Health Survey [23].

Collection of swabs and microbiologic methods

Nasal swabs were obtained from the selected subjects. Humidified swabs were inserted in both nares as deeply as possible, until the subjects reported discomfort. Specimens were transported in Stuart medium and cultured in Baird Parker agar. Species identification was performed as follows: The isolates obtained were stained by the Gram method for the determination of purity and observation of morphology and specific staining. After confirmation of these characteristics, the strains were submitted to catalase and coagulase tests. Fermentation tests of maltose, trehalose and mannitol were performed to differentiate S. aureus from other coagulase-positive staphylococci [24]. The isolates were also submitted to genotypic identification by Polymerase Chain Reaction (PCR), using primers for amplification of chromosomal DNA fragment which is specific for S. aureus (442-bp fragment). We followed the protocol described by Martineau et al. [25] and used primers Sa442-1 (5′-AAT CTT TGT CGG TAC ACG ATA TTC TTC ACG-3′) and Sa442-2 (5′-CGT AAT GAG ATT TCA GTA GAT AAT ACA ACA-3′).

Susceptibility tests followed guidelines from the Clinical Laboratory Standards Institute (CLSI), using disks for oxacillin and cefoxitin [26].

Molecular detection and characterization of SCCmec

For the purposes of our study, S. aureus isolates were classified as MRSA when they harbored the mecA gene, which is responsible for methicillin-resistance. PCR for detection of mecA was performed as described by Murakami et al. [27]. All reactions included positive (S. aureus ATCC 33591) and negative (S. aureus ATCC 25923) controls. The multiplex-PCR protocol described by Milheiriço et al [28] was used for the characterization of the staphylococcal cassette chromosome mec (SCCmec).

Molecular strain typing

All MRSA isolates were submitted to strain typing. For Pulsed-Field Gel Electrophoresis (PFGE), we used a protocol modified from McDoughal et al. [29], based on DNA digestion with the restriction enzyme SmaI. The analysis of similarity was performed using the Dice coefficient. Clusters were defined on the basis of similarity values over 80%. Dendrograms were drawn based on Unweighted Pair Group Method Using Arithmetic Averages (UPGMA) in the BioNumerics 6.1 software (Applied Maths, Belgium). Several MRSA isolates originated from different countries were included as controls in the dendrogram.

Multilocus sequence typing (MLST) was subsequently performed, amplifying and sequencing genes arcC, aroE, glpF, gmk, pta, tpi and yqiL, as described by Enright et al. [30]. Analysis of results focused on the consensus sequence, according to data from S. aureus official MLST site (http://saureus.mlst.net).

spa typing was performed according to the protocol described by Shopsin et al [31]. PCR was performed using primers 1095F (5′-AGACGATCCTTCGGTGAGC-3′) e 1517R (5′-GCTTTTGCAATGTCATTTACTG-3′). The amplified product was purified and sequenced. The analysis of repetitions was carried out in the software Bionumerics (Applied Maths, Belgium), which assessed (through specific plugin) the database from Ridom Spa Server (http://www.spaserver.ridom.de).

Detection of genes coding for PVL

All S. aureus isolates were tested for the presence of genes coding for PVL (lukS-PV, lukF-PV). We used a PCR protocol, as previously described [32].

Epidemiological analysis

A questionnaire was applied to all study subjects. Several issues were assessed, including demographics, practice of sports, behavioral factors and comorbidities. Subjects were also questioned about infectious diseases, use of antimicrobials and exposure to healthcare settings during the previous year.

All collected data were stored in database in EPI INFO 3.5 (Centers for Disease Control and Prevention, Atlanta, GA, USA) and analyzed using SPSS 19.0 (IBM, Armonk, NY, USA). Analysis was performed separately for two outcomes: (a) overall S. aureus carriage and (b) carriage of MRSA.

All data were initially submitted to bivariate analysis. Dichotomous variables were analyzed using the Chi-square or Fisher Exact test. For continuous variables, we used Student's T or the Mann-Whitney U test. Multivariate analysis was performed using logistic regression models. A “change-in-estimate” approach use used for selection of variables [33]. In the first step, logistic regression included all variables with p<0.1 in the bivariate analysis. New models included all the other variables, one at a time. Variables that changed the Odds Ratio of significant factors in more than 10% were kept in the final model. As counterproof, we performed the multivariate analysis using forward and backward strategies for variable selection, and obtained similar results. P<0.05 was used for definition of statistical significance in all steps of statystical analysis.

Results

The overall prevalence of nasal carriage of S. aureus was 32.7% (95%CI, 29.2%–36.2%). The bivariate analysis of factors associated with carriage is presented in Table 1. Table 2 displays the final multivariate logistic regression model. Briefly, persons carrying S. aureus were significantly younger than non-carriers, and more likely to report recent skin infection. Genes coding for PVL were found in five out of 224 isolates (2.2%), all of which were methicillin-susceptible. It is worth noting that only one out of five subjects carrying PVL-positive isolates reported recent skin infection. Given the small number of subjects, the incidence of skin infections in the previous year for carriers of PVL-positive (20%) and PVL-negative (11%) isolates was not statistically significant (p = 0.5).

thumbnail
Table 1. Bivariate analysis of factors associated with nasal carriage of Staphylococcus aureus among persons living in the city of Botucatu, Brazil, alongside with data from six MRSA carriers in that population.

https://doi.org/10.1371/journal.pone.0092537.t001

thumbnail
Table 2. Final model of multivariate analysis of factors associated with nasal carriage of Staphylococcus aureus among persons living in the city of Botucatu, Brazil.

https://doi.org/10.1371/journal.pone.0092537.t002

On the other hand, MRSA isolates were found colonizing the nares of 0.9% of the study subjects (95%CI, 0.4%–1.8%). None of the six MRSA-colonized subjects reported recent exposure to healthcare settings. All their household contacts (15 persons) were screened, and carriage of MRSA was found in two of them.

All MRSA isolates harbored SCCmec type IV, but none of them carried genes coding for PVL. There was a major PFGE cluster grouping isolates from four out of six MRSA-positive subjects, plus one household contact (Figure 1). The isolates in that cluster were characterized as spa type t002. In MLST, most belonged to ST5. However, one isolated differed in the allele for the gene glpF. The data were submitted to the MSLT homepage (http://saureus.mlst.net), and a novel number was attributed to that allele (320). The isolated was characterized as a novel clone (ST 2594).

thumbnail
Figure 1. Dendrogram showing Pulsed Field Gel Electrophoresis typing of the study isolates, alongside with results from Multilocus Sequence Typing and spa typing.

International SCCmec type IV clones are included as controls. Note. Data on age/gender and urban area typing are included. Control strains were kindly provided by Dr. Antonio Carlos Campos Pignatari (Universidade Federal de São Paulo, City of São Paulo, São Paulo State, Brazil) and Dr. Agnes Marie Sá Figueiredo (Universidade Federal do Rio de Janeiro, City of Rio de Janeiro, Rio de Janeiro State, Brazil).

https://doi.org/10.1371/journal.pone.0092537.g001

The two remaining subjects carried MRSA isolates that presented different PFGE patterns and spa types (t002 and t021), but belonged to ST1176. One of them had a household contact colonized with an isolate that was similar to hers in all typing methods.

Discussion

Several recent studies have assessed S. aureus asymptomatic colonization among non-hospitalized persons. Many among them focused on special populations, such as sportsmen [34], military [35], prison inmates [36] or remote communities [37], [38]. Others enrolled specific age groups, such as children [39], [40], [41] and elderly persons [41]. While these studies are of great interest, it is still necessary to estimate the burden of S. aureus and MRSA carriage among general population from different countries. Our study aimed to contribute to this issue.

This was the first study to focus S. aureus colonization in Brazil using a population-based approach. Our findings point out to a prevalence of both S. aureus and MRSA colonization similar to that reported in the 2001–2002 NHANES reports [16], [17] and in minor studies from the U.S. [42], [43]. We found that about one third of the population of Botucatu carries S. aureus on their nares. On the other hand, the spread of MRSA in the community is limited. Still, we can estimate more than 1,000 persons carrying MRSA in the city of Botucatu at the time when the study was conducted. This is in contrast with results from two studies that focused on asymptomatic S. aureus carriage in Europe. The Tromso Staph and Skin Study enrolled 2,279 subjects, finding prevalence of S. aureus nasal colonization of 26.2% for healthcare workers and 26.0% for the general population [44]. Pooled results from Dutch surveys reported colonization in 34.9% of 3,198 children and 27.1% of 3,851 adults over 55 years of age [41]. Despite their sample size, neither studies from Norway or Netherlands found any case of MRSA colonization. At the other end of the epidemiological spectrum in Europe, one study from Malta reported MRSA nasal colonization in 8.8% of 329 healthy individuals [45].

The comparison of our results with data from other countries or continents is difficult, due to differences in the target populations and sites of collection of cultures. Still, it is worth reporting that surveys in African countries found asymptomatic colonization with S. aureus and a whole and MRSA in 33% and 9% of 120 persons from the Niger delta (Nigeria) [46] and in 29% and 1% of 500 subjects from rural and semi-urban areas in Lambaréné, Gabon [47]. One study from Queensland (Australia) enrolled 396 patients presenting to general practice and 303 randomly selected volunteers. The prevalence rates of methicillin-susceptible S. aureus and MRSA nasal colonization were 28% and 0.7% [48].

Hamdan-Partida et al. [49] investigated 1,243 healthy volunteers from Mexico, finding S. aureus and MRSA in 59.8% and 8.6% of study subjects. Those authors followed subjects for 6-years in order to characterize persistent carriage, which occurred in 13.0% of colonized persons. Cultures from nares and throat were performed, and this strategy increased the detection of S. aureus. However, the study subjects were selected on the basis of the possibility of long term follow up. Therefore, the sample was not stratified in order to reflect the demographic distribution of Mexican population.

In a study from India, the reported prevalence rates among 1,000 subjects were 22.5% for S. aureus and 16.6% for MRSA [50]. A comparison of these results with ours is hindered by the fact that researchers also screened subjects for colonization in body sites other than the nares: namely, forearms and palms. Once again, the approach used for selection of subjects is unclear. Several groups were enrolled, including people from different economic classes, healthcare workers and inpatients. The latter two groups seem overrepresented, so one may doubt the validity of the results as estimates of the colonization burden in the general population.

In our study, we found an inverse association between age and S. aureus colonization. This finding has been consistently reported in other studies [16], [18]. Indeed, research that specifically focused on children identified high rates of both S. aureus and MRSA colonization [51], [52]. Interestingly, a survey conducted among Pygmies from Gabon identified peek colonization rates among teenagers [38].

In our study, S. aureus colonization was associated with the report of skin infections in the past year. In fact, incidence of skin infections during that period was 11.2% for carriers of S. aureus. Also, those infections affected two out of six MRSA-colonized subjects. An interesting result is obtained when we reanalyze our database, selecting skin infection as an outcome, and including alternatively colonization with S. aureus as a whole or MRSA in multivariate logistic regression models. In this analysis, the previous history of skin infections is independently associated both with the carriage of S. aureus (OR, 2.04; 95%CI, 1.11–3.73; p = 0.02) and of MRSA (OR, 9.19; 95%CI, 1.158–53.47; p = 0.01). On the other hand, the presence of PVL genes was not associated to skin infections, possibly due to the small number of isolates harboring those genes.

The association of nasal colonization with S. aureus and skin or soft tissue infections was reported as early as the 1960s [53]. In the following decades, studies related colonization with chronic furunculosis and surgical site infections [54], [55], [56], [57]. Based on those results, decolonization with mupirocin has been advised for preventing recurrent skin infections [55], [57].

However, this issue remains controversial [58]. Some studies reported association of S. aureus or MRSA colonization with several non-infectious skin diseases [59], [60], [61]. High prevalence of MRSA (of community or healthcare origin) carriage was found in the nares and skin of patients from a dermatology outpatient service [62]. On the other hand, nasal decolonization has failed to prevent recurrent skin infections [63]. Finally, studies focusing on the association of skin infections with colonization of patients and their households reported conflicting results [64], [65], [66].

In regard to our results, as well as those reported in the papers discussed above, one must remember that data obtained from cross-section studies are ambiguous in regard to causality. It is therefore possible that carriage of S. aureus led to the development of infection, or that colonization just remained after the infectious syndrome was controlled.

Though limited, our data on MRSA colonization suggest that there are few clones spreading in the community, possibly through social contact. All isolates harbored SCCmec type IV, and belonged to Clonal Complex 5 (ST5, ST1176 or ST2594) on MLST. Most of them belonged to the same spa type (t002). Finally, a major PFGE cluster comprised isolates from four out of six subjects. Interestingly, two MRSA carriers (subjects #103 and #106) lived on the same street, and their isolates had 100% electrophoretic similarity. Also, two household contacts were colonized with strains similar to those found in their related subjects.

Those findings are noteworthy. Clonal complex 5 is widely spread through America, Europe, Asia and Australia [67]. ST1176 was recently described on clinical cultures from patients admitted to hospitals in the city of São Paulo, 140 miles apart from Botucatu [68]. ST2594 is a novel clone, identified in our survey, and belongs to Clonal complex 5. In summary, our study found evidence of clonal spread on regional and household levels.

The routes of S. aureus spread in the community are yet to be elucidated. The same is true for CA-MRSA clones, in spite of extensive research applying methods from classic and molecular epidemiology [19]. It has been suggested that methodological approaches based on social networks may help in filling gaps in our current knowledge. An interesting study addressed this issue focusing on the most basic social network: the household [64]. Analysis of determinants and clonality of S. aureus colonization was performed both on individual and aggregate levels. The authors found evidence for transmission within the household, but also for continuous introduction of new strains into households.

Another interesting result concerns PVL. Genes coding for this virulence factor were found in few S. aureus isolates, all of which were methicillin-susceptible. The absence of PVL in MRSA isolates colonizing asymptomatic persons is not surprising. In fact, PVL was not an effective marker for CA-MRSA in all studies [69]. Besides, it is expected that PVL-harboring clones are more prevalent among people with active infections.

Our study has several limitations. Most are inherent to the cross-sectional design, which did not allow us to assess the acquisition of S. aureus and MRSA over time. Also, new evidence pointed out that relying only on nasal samples may have underestimated the colonization prevalence [49]. The scarcity of subjects with the outcome cast doubt on the validity of the analysis of determinants of MRSA colonization. Finally, authors have questioned the role of nasal colonization as a major issue in CA-MRSA epidemiology [70]. Analyzing data from outbreak reports, they conclude that skin-skin and skin-fomite contact may provide an efficient route for cross-transmission, even in the absence of colonization.

Despite all those limitations, our study has several strengths. First, subjects were selected so as to provide a representative sample of the urban population of the city of Botucatu. Several potential determinants of colonization, including previous contact with healthcare settings, were assessed in the questionnaire. The analysis was extensive, and mixed classical epidemiological and molecular methods to provide the first data on population carriage of S. aureus and MRSA in Brazil. Our findings are particularly relevant at a time when CA-MRSA reaches the status of a public health threat. There are lots of issues to be addressed, including policies for screening patients upon admission to hospitals and guidelines for treating suspected staphylococcal infections. Also, surveillance for CA-MRSA infection should be implemented on a nation-wide basis, in order to make sure if data from asymptomatic MRSA carriage correlate with the etiology of skin and soft-tissue infections. It is worth reporting that one adolescent with CA-MRSA cellulitis that started after trauma in a soccer match and progressed to severe sepsis was diagnosed in Bofete, a small town neighboring Botucatu. The isolate belonged to ST5, spa type 311, and harbored PVL genes [71]. As with our results, this occurrence demonstrates the circulation of CA-MRSA in regions distant from major urban centers, and reinforces the necessity of including CA-MRSA in the agenda of public health offices in Brazil.

In conclusion, we found that prevalence rates of S. aureus and MRSA colonization in a small city in inner Brazil were similar to those reported in nationwide US surveys. There is evidence of low-level (but consistent) spread CA-MRSA clones, a finding that deserves further investigation. Our results demonstrate the importance of including CA-MRSA among pathogens of interest for public health surveillance at the regional level.

Acknowledgments

Disclaimer: Partial results were presented at ID week (joint Meeting of IDSA, SHEA, HIVMA, and PIDS) in San Diego, 2012.

Author Contributions

Conceived and designed the experiments: CMCBF MLRSC FVP. Performed the experiments: FVP LMA PYFM CHC. Analyzed the data: CMCBF MLRSC CHC. Contributed reagents/materials/analysis tools: PYFM CHC LMA. Wrote the paper: CMCBF MLRSC FVP.

References

  1. 1. Hayward A, Knott F, Petersen I, Livermore DM, Duckworth G, et al. (2008) Increasing hospitalizations and general practice prescriptions for community-onset staphylococcal disease, England. Emerg Infect Dis 14: 720–726.
  2. 2. Saxena S, Thompson P, Birger R, Bottle A, Spyridis N, et al. (2010) Increasing skin infections and Staphylococcus aureus complications in children, England, 1997–2006. Emerg Infect Dis 16: 530–533.
  3. 3. Grundmann H, Aires-de-Sousa M, Boyce J, Tiemersma E (2006) Emergence and resurgence of meticillin-resistant Staphylococcus aureus as a public-health threat. Lancet 368: 874–885.
  4. 4. Bassetti M, Nicco E, Mikulska M (2009) Why is community-associated MRSA spreading across the world and how will it change clinical practice? Int J Antimicrob. Agents 34 (Suppl 1)S15–S19.
  5. 5. Klevens RM, Morrison MA, Nadle J, Petit S, Gershman K, et al. (2007) MRSA Investigators. Invasive methicillin-resistant Staphylococcus aureus infections in the United States. JAMA 298: 1763–1771.
  6. 6. Köck R, Becker K, Cookson B, van Gemert-Pijnen JE, Harbarth S, et al. (2010) Methicillin-resistant Staphylococcus aureus (MRSA): burden of disease and control challenges in Europe. Euro Surveill 15: 19688.
  7. 7. Chuang YY, Huang YC (2013) Molecular epidemiology of community-associated meticillin-resistant Staphylococcus aureus in Asia. Lancet Infect Dis 13: 698–708.
  8. 8. Breurec S, Zriouil SB, Fall C, Boisier P, Brisse S, et al. (2011) Epidemiology of methicillin-resistant Staphylococcus aureus lineages in five major African towns: emergence and spread of atypical clones. Clin Microbiol Infect 17: 160–165.
  9. 9. Breurec S, Fall C, Pouillot R, Boisier P, Brisse S, et al. (2011) Epidemiology of methicillin-susceptible Staphylococcus aureus lineages in five major African towns: high prevalence of Panton-Valentine leukocidin genes. Clin Microbiol Infect 17: 633–639.
  10. 10. Casey JA, Cosgrove SE, Stewart WF, Pollak J, Schwartz BS (2013) A population-based study of the epidemiology and clinical features of methicillin-resistant Staphylococcus aureus infection in Pennsylvania, 2001–2010. Epidemiol Infect 141: 1166–1179.
  11. 11. Johnson AP (2011) Methicillin-resistant Staphylococcus aureus: the European landscape. J Antimicrob. Chemother. 66 (Suppl 4)iv43–iv48.
  12. 12. Mithoe D, Rijnders MI, Roede BM, Stobberingh E, Möller AV (2012) Prevalence of community-associated meticillin-resistant Staphylococcus aureus and Panton-Valentine leucocidin-positive S. aureus in general practice patients with skin and soft tissue infections in the northern and southern regions of The Netherlands. Eur J Clin Microbiol Infect Dis 31: 349–56.
  13. 13. Wu D, Wang Q, Yang Y, Geng W, Wang Q, et al. (2010) Epidemiology and molecular characteristics of community-associated methicillin-resistant and methicillin-susceptible Staphylococcus aureus from skin/soft tissue infections in a children's hospital in Beijing, China. Diagn Microbiol Infect Dis 67: 1–8.
  14. 14. Kluytmans J, van Belkum A, Verbrugh H (1997) Nasal carriage of Staphylococcus aureus: epidemiology, underlying mechanisms, and associated risks. Clin Microbiol Rev 10: 505–520.
  15. 15. Safdar N, Bradley EA (2008) The risk of infection after nasal colonization with Staphylococcus aureus. Am J Med 121: 310–315.
  16. 16. Kuehnert MJ, Kruszon-Moran D, Hill HA, McQuillan G, McAllister SK, et al. (2006) Prevalence of Staphylococcus aureus nasal colonization in the United States, 2001–2002. J Infect Dis 193: 172–179.
  17. 17. Graham PL 3rd, Lin SX, Larson EL (2006) A U.S. population-based survey of Staphylococcus aureus colonization. Ann Intern Med 144: 318–325.
  18. 18. Gorwitz RJ, Kruszon-Moran D, McAllister SK, McQuillan G, McDougal LK, et al. (2008) Changes in the prevalence of nasal colonization with Staphylococcus aureus in the United States, 2001–2004. J Infect Dis 197: 1226–1234.
  19. 19. Mediavilla JR, Chen L, Mathema B, Kreiswirth BN (2012) Global epidemiology of community-associated methicillin resistant Staphylococcus aureus (CA-MRSA). Curr Opin Microbiol 15: 588–595.
  20. 20. Ribeiro A, Coronado AZ, Silva-Carvalho MC, Ferreira-Carvalho BT, Dias C, et al. (2007) Detection and characterization of international community-acquired infections by methicillin-resistant Staphylococcus aureus clones in Rio de Janeiro and Porto Alegre cities causing both community- and hospital-associated diseases. Diagn Microbiol Infect Dis 59: 339–345.
  21. 21. Silva-Carvalho MC, Bonelli RR, Souza RR, Moreira S, dos Santos LC, et al. (2009) Emergence of multiresistant variants of the community-acquired methicillin-resistant Staphylococcus aureus lineage ST1-SCCmecIV in 2 hospitals in Rio de Janeiro, Brazil. Diagn Microbiol Infect Dis 65: 300–305.
  22. 22. Reinert C, McCulloch JA, Watanabe S, Ito T, Hiramatsu K, et al. (2008) Type IV SCCmec found in decade old Brazilian MRSA isolates. Braz J Infect Dis 12: 213–236.
  23. 23. Cesar CL, Carandina L, Alves MC, Barros MB, Goldbaum M (2005) Saúde e condição de vida em São Paulo. Inquérito multicêntrico de saúde no estado de São Paulo – ISA/SP. São Paulo, Brazil: Universidade de São Paulo, Faculdade de Saúde Pública. 212 p.
  24. 24. Winn Jr WM, Allen S, Janda W, Koneman E, Procop G, et al. (2005) Koneman's Color Atlas and Textbook of Diagnostic Microbiology, 6th Ed. Philadelphia: Lippincott Williams & Wilkins. 1,736p.
  25. 25. Martineau F, Picard FJ, Roy PH, Ouellette M, Bergeron MG (1998) Species-specific and ubiquitous-DNA-based assays for rapid identification of Staphylococcus aureus. J Clin Microbiol. 6: 618–23.
  26. 26. Clinical and Laboratory Standards Institute (2009) Performance standards for antimicrobial disk susceptibility tests; approved standard, tenth edition (M02-A10). Wayne, PA: CLSI. 76 p.
  27. 27. Murakami K, Minamide K, Wada K, Nakamura E, Teraoka H, et al. (1991) Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction. J Clin Microbiol 29: 2240–2244.
  28. 28. Milheiriço C, Oliveira DC, de Lencastre H (2007) Update to the multiplex PCR strategy for assignment of mec element types in Staphylococcus aureus. Antimicrob. Agents Chemother 51: 3374–3377.
  29. 29. McDougal LK, Steward CD, Killgore GE, Chaitram JM, McAllister SK, et al. (2003) Pulsed-field gel electrophoresis typing of oxacillin-resistant Staphylococcus aureus isolates from the United States: establishing a national database. J Clin Microbiol. 41: 5113–5120.
  30. 30. Enright MC, Day NP, Davies CE, Peacock SJ, Spratt BG (2000) Multilocus sequence typing for characterization of methicillin- resistant and methicillin-susceptible clones of Staphylococcus aureus. J Clin Microbiol 38: 1008–1015.
  31. 31. Shopsin B, Gomes M, Montomery SO, Smith DH, Waddington M, et al. (1999) Evaluation of Protein A Gene Polymorphic Region DNA Sequencing for Typing of Staphylococcus aureus Strains. J Clin Microbiol 37: 3556–3563.
  32. 32. Lina G, Piémont Y, Godail-Gamot F, Bes M, Peter MO, et al. (1999) Involvement of Panton-Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia. Clin Infect Dis 29: 1128–1132.
  33. 33. Greenland S (1989) Modeling and variable selection in epidemiologic analysis. Am J Public Health 79: 340–349.
  34. 34. Lear A, McCord G, Peiffer J, Watkins RR, Parikh A, et al. (2011) Incidence of Staphylococcus aureus nasal colonization and soft tissue infection among high school football players. J Am Board Fam Med 24: 429–435.
  35. 35. Qu F, Cui E, Guo T, Li H, Chen S, et al. (2010) Nasal colonization of and clonal transmission of methicillin-susceptible Staphylococcus aureus among Chinese military volunteers. J Clin Microbiol 48: 64–69.
  36. 36. Lee CJ, Sankaran S, Mukherjee DV, Apa ZL, Hafer CA, et al. (2011) Staphylococcus aureus oropharyngeal carriage in a prison population. Clin Infect Dis 52: 775–778.
  37. 37. O'Brien FG, Coombs GW, Pearman JW, Gracey M, Moss F, et al. (2009) Population dynamics of methicillin-susceptible and -resistant Staphylococcus aureus in remote communities. J Antimicrob Chemother 64: 684–693.
  38. 38. Schaumburg F, Köck R, Friedrich AW, Soulanoudjingar S, Ngoa UA, et al. (2011) Population structure of Staphylococcus aureus from remote African Babongo Pygmies. PLoS Negl Trop Dis 5: e1150.
  39. 39. Tavares DA, Sá-Leão R, Miragaia M, de Lencastre H (2010) Large screening of CA-MRSA among Staphylococcus aureus colonizing healthy young children living in two areas (urban and rural) of Portugal. BMC Infect Dis 10: 110.
  40. 40. Chen CJ, Hsu KH, Lin TY, Hwang KP, Chen PY, et al. (2011) Factors associated with nasal colonization of methicillin-resistant Staphylococcus aureus among healthy children in Taiwan. J Clin Microbiol. 49: 131–137.
  41. 41. Melles DC, Tenover FC, Kuehnert MJ, Witsenboer H, Peeters JK, et al. (2008) Overlapping population structures of nasal isolates of Staphylococcus aureus from healthy Dutch and American individuals. J Clin Microbiol. 46: 235–241.
  42. 42. Rim JY, Bacon AE 3rd (2007) Prevalence of community-acquired methicillin-resistant Staphylococcus aureus colonization in a random sample of healthy individuals. Infect Control Hosp Epidemiol 28: 1044–6.
  43. 43. Malik S, Vranken P, Silio M, Ratard R, Van Dyke R (2009) Prevalence of community-associated methicillin-resistant Staphylococcus aureus colonization outside the healthcare environment. Epidemiol Infect 137: 1237–1241.
  44. 44. Olsen K, Sangvik M, Simonsen GS, Sollid JU, Sundsfjord A, et al. (2013) Prevalence and population structure of Staphylococcus aureus nasal carriage in healthcare workers in a general population. The Tromsø Staph and Skin Study. Epidemiol Infect 141: 143–152.
  45. 45. Scerri J, Monecke S, Borg MA (2013) Prevalence and characteristics of community carriage of methicillin-resistant Staphylococcus aureus in Malta. J Epidemiol Glob Health 3: 165–173.
  46. 46. Onanuga A, Temedie TC (2011) Nasal carriage of multi-drug resistant Staphylococcus aureus in healthy inhabitants of Amassoma in Niger delta region of Nigeria. Afr Health Sci 11: 176–181.
  47. 47. Ateba Ngoa U, Schaumburg F, Adegnika AA, Kösters K, Möller T, et al. (2012) Epidemiology and population structure of Staphylococcus aureus in various population groups from a rural and semi urban area in Gabon, Central Africa. Acta Trop 124: 42–47.
  48. 48. Munckhof WJ, Nimmo GR, Schooneveldt JM, Schlebusch S, Stephens AJ, et al. (2009) Nasal carriage of Staphylococcus aureus, including community-associated methicillin-resistant strains, in Queensland adults. Clin Microbiol Infect 15: 149–55.
  49. 49. Hamdan-Partida A, Sainz-Espuñes T, Bustos-Martínez (2010) Characterization and persistence of Staphylococcus aureus strains isolated from the anterior nares and throats of healthy carriers in a Mexican community. J Clin Microbiol 48: 1701–1705.
  50. 50. Goud R, Gupta S, Neogi U, Agarwal D, Naidu K, et al. (2011) Community prevalence of methicillin and vancomycin resistant Staphylococcus aureus in and around Bangalore, southern India. Rev Soc Bras Med Trop 44: 309–312.
  51. 51. Gardella N, Murzicato S, Di Gregorio S, Cuirolo A, Desse J, et al. (2011) Prevalence and characterization of methicillin-resistant Staphylococcus aureus among healthy children in a city of Argentina. Infect Genet Evol 11: 1066–71.
  52. 52. Adler A, Givon-Lavi N, Moses AE, Block C, Dagan R (2010) Carriage of community-associated methicillin-resistant Staphylococcus aureus in a cohort of infants in southern Israel: risk factors and molecular features. J Clin Microbiol 48: 531–8.
  53. 53. Williams RE (1963) Healthy carriage of Staphylococcus aureus: its prevalence and importance. Bacteriol Rev 27: 56–71.
  54. 54. Weems JJ, Beck LB (2002) Nasal Carriage of Staphylococcus aureus As a Risk Factor for Skin and Soft Tissue Infections. Curr Infect Dis Rep 4: 420–425.
  55. 55. Durupt F, Mayor L, Bes M, Reverdy ME, Vandenesch F, et al. (2007) Prevalence of Staphylococcus aureus toxins and nasal carriage in furuncles and impetigo. Br J Dermatol 157: 1161–1167.
  56. 56. Fritz SA, Epplin EK, Garbutt J, Storch GA (2009) Skin infection in children colonized with community-associated methicillin-resistant Staphylococcus aureus. J Infect 59: 394–401.
  57. 57. Demos M, McLeod MP, Nouri K (2012) Recurrent furunculosis: a review of the literature. Br J Dermatol 167: 725–732.
  58. 58. Smith CH, Goldman RD (2012) Staphylococcus aureus decolonization for recurrent skin and soft tissue infections in children. Can Fam Physician 58: 1350–1352.
  59. 59. Ertam I, Biyikli SE, Yazkan FA, Aytimur D, Alper S (2007) The frequency of nasal carriage in chronic urticaria patients. J Eur Acad Dermatol Venereol 21: 777–780.
  60. 60. Talpur R, Bassett R, Duvic M (2008) Prevalence and treatment of Staphylococcus aureus colonization in patients with mycosis fungoides and Sézary syndrome. Br J Dermatol 159: 105–112.
  61. 61. Petry V, Lipnharski C, Bessa GR, Silveira VB, Weber MB, et al. (2013). Prevalence of community-acquired methicillin-resistant Staphylococcus aureus and antibiotic resistance in patients with atopic dermatitis in Porto Alegre, Brazil. Int J Dermatol. E-pub ahead of print. doi:10.1111/ijd.12020
  62. 62. Pacheco RL, Lobo RD, Oliveira MS, Farina EF, Santos CR, et al. (2011) Methicillin-resistant Staphylococcus aureus (MRSA) carriage in a dermatology unit. Clinics (Sao Paulo) 66: 2071–2077.
  63. 63. Rahimian J, Khan R, LaScalea KA (2007) Does nasal colonization or mupirocin treatment affect recurrence of methicillin-resistant Staphylococcus aureus skin and skin structure infections? Infect Control Hosp Epidemiol 28: 1415–1416.
  64. 64. Miller M, Cook HA, Furuya EY, Bhat M, Lee MH, et al. (2009) Staphylococcus aureus in the community: colonization versus infection. PLoS One 4: e6708.
  65. 65. Fritz SA, Hogan PG, Hayek G, Eisenstein KA, Rodriguez M, et al. (2012) Staphylococcus aureus colonization in children with community-associated Staphylococcus aureus skin infections and their household contacts. Arch Pediatr Adolesc Med 166: 551–557.
  66. 66. Fritz SA, Hogan PG, Hayek G, Eisenstein KA, Rodriguez M, et al. (2012) Household versus individual approaches to eradication of community-associated Staphylococcus aureus in children: a randomized trial. Clin Infect Dis 54: 743–51.
  67. 67. Monecke S, Coombs G, Shore AC, Coleman DC, Akpaka P, et al. (2011) A field guide to pandemic, epidemic and sporadic clones of methicillin-resistant Staphylococcus aureus. PLoS One 6: e17936.
  68. 68. Carmo MS, Inoue F, Andrade SS, Paschoal L, Silva FM, et al. (2011) New multilocus sequence typing of MRSA in São Paulo, Brazil. Braz J Med Biol Res 44: 1013–1017.
  69. 69. Rossney AS, Shore AC, Morgan PM, Fitzgibbon MM, O'Connell B, et al. (2007) The emergence and importation of diverse genotypes of methicillin-resistant Staphylococcus aureus (MRSA) harboring the Panton-Valentine leukocidin gene (pvl) reveal that pvl is a poor marker for community-acquired MRSA strains in Ireland. J Clin Microbiol 45: 2554–2563.
  70. 70. Miller LG, Diep BA (2008) Clinical practice: colonization, fomites, and virulence: rethinking the pathogenesis of community-associated methicillin-resistant Staphylococcus aureus infection. Clin Infect Dis 46: 752–760.
  71. 71. Camargo CH, da Cunha M de L, Bonesso MF, da Cunha FP, Barbosa AN, et al. (2013) Systemic CA-MRSA infection following trauma during soccer match in inner Brazil: clinical and molecular characterization. Diagn Microbiol Infect Dis 76: 372–374.