23 Jan 2014: de Luis M, Čufar K, Di Filippo A, Novak K, Papadopoulos A, et al. (2014) Correction: Plasticity in Dendroclimatic Response across the Distribution Range of Aleppo Pine (Pinus halepensis). PLOS ONE 9(1): 10.1371/annotation/5e1bcd37-a7f8-4f8b-b621-e58bb3caea90. https://doi.org/10.1371/annotation/5e1bcd37-a7f8-4f8b-b621-e58bb3caea90 View correction
We investigated the variability of the climate-growth relationship of Aleppo pine across its distribution range in the Mediterranean Basin. We constructed a network of tree-ring index chronologies from 63 sites across the region. Correlation function analysis identified the relationships of tree-ring index to climate factors for each site. We also estimated the dominant climatic gradients of the region using principal component analysis of monthly, seasonal, and annual mean temperature and total precipitation from 1,068 climatic gridpoints. Variation in ring width index was primarily related to precipitation and secondarily to temperature. However, we found that the dendroclimatic relationship depended on the position of the site along the climatic gradient. In the southern part of the distribution range, where temperature was generally higher and precipitation lower than the regional average, reduced growth was also associated with warm and dry conditions. In the northern part, where the average temperature was lower and the precipitation more abundant than the regional average, reduced growth was associated with cool conditions. Thus, our study highlights the substantial plasticity of Aleppo pine in response to different climatic conditions. These results do not resolve the source of response variability as being due to either genetic variation in provenance, to phenotypic plasticity, or a combination of factors. However, as current growth responses to inter-annual climate variability vary spatially across existing climate gradients, future climate-growth relationships will also likely be determined by differential adaptation and/or acclimation responses to spatial climatic variation. The contribution of local adaptation and/or phenotypic plasticity across populations to the persistence of species under global warming could be decisive for prediction of climate change impacts across populations. In this sense, a more complex forest dynamics modeling approach that includes the contribution of genetic variation and phenotypic plasticity can improve the reliability of the ecological inferences derived from the climate-growth relationships.
Citation: de Luis M, Čufar K, Di Filippo A, Novak K, Papadopoulos A, Piovesan G, et al. (2013) Plasticity in Dendroclimatic Response across the Distribution Range of Aleppo Pine (Pinus halepensis). PLoS ONE 8(12): e83550. https://doi.org/10.1371/journal.pone.0083550
Editor: Ben Bond-Lamberty, DOE Pacific Northwest National Laboratory, United States of America
Received: June 3, 2013; Accepted: November 5, 2013; Published: December 31, 2013
Copyright: © 2013 de Luis et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was partially supported by Spanish Ministry of Education and Science co-funded by FEDER program (CGL2012-31668), the European Union and the National Ministry of Education and Religion of Greece (EPEAEK- Environment – Archimedes), the Slovenian Research Agency (program P4-0015), and the USDA Forest Service. The cooperation among international partners was supported by the COST Action FP1106, STREeSS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Climate strongly influences the geographical distribution of plant species in terrestrial ecosystems , . As local temperature and water availability change with broader changes in climate, biodiversity, species distribution, and growth rates are expected to change accordingly , , . However, the impact of climate change may vary greatly depending on the ability of species to acclimate or to adapt to future climate conditions .
Plant species can adjust to new environmental conditions through local adaptation or phenotypic plasticity . Local adaptation may imply genetic differentiation among populations as a consequence of differential selection pressures and/or population isolation . Phenotypic plasticity, defined as the range of phenotypes that a single genotype can express as a function of its environment, can also be a crucial factor for plant response to rapid climate change , . Heterogeneous responses of species to climate variability across their range are directly connected to phenomena of local adaptation and phenotypic plasticity and are the basis of potential adaptability to future climate conditions. Detailed knowledge of the relationship between climate and growth across the range of distribution of species is essential to predict and mitigate the effects of climate change.
Climate change is especially rapid and extreme in the Mediterranean basin , , . However, due to the transitional nature of the Mediterranean climate (which ranges from near-desert to temperate regimes), Mediterranean areas contain a great variety of natural conditions which will likely affect the impact of climate change , , .
In the widely diverse set of climatic conditions contained in the Mediterranean basin, pines are by far the most widespread genus covering approximately 5% of the total land area and 25% of the forested area. The most common species is the Aleppo pine (Pinus halepensis Mill.) occupying large areas of the western Mediterranean as well as occurring in the eastern Mediterranean , . The ecology and biogeography of Aleppo pine is well-researched , . The dendroclimatology of Aleppo pine has been investigated at individual locations within the Mediterranean basin , , , , , , , , , ,  confirming that Aleppo pine is sensitive to climate variation. However, a comprehensive dendroclimatic analysis across the distribution range of Aleppo pine is lacking.
In this paper, we investigate the variability of the climate-growth relationships of Aleppo pine using a dendroclimatic network composed of high-resolution climatic and dendrochronology databases comprising the complete climatic gradient across the Mediterranean Basin. Our objectives were to a) characterize the climatic variability within the range of Aleppo pine; b) relate high frequency variation in tree-ring width to monthly, seasonal, and annual temperature and precipitation; c) determine the dependence of the dendroclimatic relationships to underlying climatic patterns.
All sampling sites were located in public forests. The necessary permits for field sampling were issued by the forestry authorities at regional and local level. The locations were not protected areas, and the field studies did not involve endangered or protected species.
Locations and climatic data
The distribution map for Aleppo pine (Fig. 1) based on an earlier map  was obtained from the European Forest Genetic Resources Programme (EUFORGEN http://www.euforgen.org/distribution_maps.html). Climatic time series data for 1,068 spatial grid points within the distribution range for the period 1901–2000 were obtained from the Climatic Research Unit (CRU) of the University of East Anglia. For the western part (Spain, Algeria, France, Italy, Slovenia, Greece, and Turkey), monthly, seasonal, and annual mean temperatures and total precipitation were provided by the CRU TS 1.2 dataset (10-minute resolution) . For the portion of the distribution located east of the coverage of CRU TS 1.2, we used the CRU TS 2.1 dataset (0.5° grid resolution) . To characterize the spatial variability of the climate across the distribution area of Aleppo pine, while minimizing the effect of outlier values, we determined the central 95% range for each variable, defined as the values occurring between the 2.5 and 97.5 ranked percentiles.
The dendrochronological network consisted of newly collected and archived tree-ring width series from 63 Aleppo pine sites (Table 1), between 32.23° and 45.67°N latitude, 1.41°W and 36.17°E longitude, and 15 to 1650 m a.s.l. (Fig. 1). To determine the adequacy of the network to describe the range of dendroclimatic responses, climatic data for the CRU gridpoint closest to each site were compared with those of the Aleppo pine distribution as a whole.
A T-mode Principal Component Analysis (PCA)  was used to summarize the spatial variability of the mean climate conditions across the study area. PCA is a data reduction technique that transforms a large group of variables into a new smaller set of variables called principal components (PC), which are linear combinations of the original variables. These PC were calculated on the correlation matrix of mean monthly temperatures and mean total monthly precipitation for the period 1901–2000. The components were rotated (Varimax) to redistribute the explained variance and to obtain more stable and robust spatial patterns . Selected PC, guided by Kaiser's Rule (eigenvalues >1; ), described climatic gradients across the Mediterranean basin were used in subsequent analysis.
Tree-ring chronology construction
The tree-ring width series were derived from a total of 1634 increment cores from 818 trees (Table 1). Tree selection, core collection, processing, and ring-width measurement were conducted using standard techniques , . Ring-width index chronologies for each site in the network were constructed using ARSTAN software version 6.05P  to retain the high-frequency variation that would be most sensitive to the climate variables to be tested. First, a negative exponential or linear regression function was fitted to each ring-width series. The residuals from this first detrending were then fitted with a cubic smoothing spline function (50% frequency cut-off of 30 years). The residuals from the second detrending were then fitted with an autoregressive model to reduce the autocorrelation in the model residuals which were then averaged to construct the ring-width residual index chronology (chronology RES in ARSTAN) for each site using the biweight robust mean function. An expressed population signal (EPS) of 0.85 was used as a threshold to determine the reliable part of each index chronology to use in subsequent analysis .
Dendroclimatic relationships across climate gradients
For our first-stage tests, the statistical relationship between monthly, seasonal and annual climate series and annual tree-ring chronologies was assessed individually for each of the 63 sites by correlation function (CF) analysis using the program DendroClim2002 . The RES chronology was the dependent variable while the regressors were the monthly, seasonal and annual mean temperatures and the total precipitation for each 16-month biological year (from the previous September to the current December).
The second-stage test consisted of multiple regression analysis (forward selection) using the CF coefficients calculated in the first stage as dependent variables and the significant principal components (PC) as independent variables that characterize mean climate conditions for each of 63 study sites. In this test, dependence of CF on PC indicates that the correlation of growth to annual, seasonal, and monthly climate variability depended on the underlying climatic conditions. Then, if dendroclimatic relations were found to be climate-dependent, the variation in the climate-growth relationship may be quantified and predicted for the whole distribution area of species by resolving obtained regression models on all 1,068 points for which PC values were calculated.
Climate characteristics within the distribution area of Aleppo pine
The climatic conditions varied widely from near desert to moist temperate. The central 95% of the range (bounded by the 0.025 and 0.975 percentiles) of mean annual temperatures ranged from 10.2°–17.9°C. Winter temperature (average of 7.0°C) ranged from 2.9–11.5°C and summer temperature (average of 22.3°C) ranged from 18.2–25.9°C (Fig. 2). The central 95% range of annual total precipitation (average of 570 mm) across the geographic distribution ranged from 328–957 mm. The seasonal distribution of precipitation varied greatly with winter precipitation comprising about 31% of the total annual precipitation. Among sites, winter precipitation contributed 15–66% of the annual total precipitation. Spring precipitation contributed an average of about 27% of the total annual precipitation with sites ranging from about 12–36%. Autumn precipitation contributed an average of about 30% with a range of about 13–43%. Summer was generally the dry season with an average annual contribution of about 11% of the total precipitation but ranged across sites from less than 0.1% to about 29% of the total annual precipitation (Fig. 2).
Dark grey and light grey areas represent climate envelopes defined by the 2.5th–97.5th and the 0.5th–99.5th percentiles, respectively. Box plots show the range of mean monthly air temperatures and total monthly precipitation for the P. halepensis dendrochronological network of 63 locations. The central vertical lines indicate median values, boxes enclose the central two quartiles, whiskers indicate the 10th and the 90th percentiles and the dots represent the full range of mean climate values.
The climate variation across the distribution area of Aleppo pine can be summarized by four significant principal components (PC) which together explain about 95% of the variation. PC1 (containing 59% of the variation) represented the main climatic gradient, from cold and wet conditions to warm and dry (Fig. 3). PC2 (about 24%) related to variation in precipitation (Fig. 3). PC3 (about 8%) represented variation in the seasonal distribution of precipitation from sites where winter is the main precipitation season to sites where the contribution of summer and autumn precipitation to the total annual precipitation increases (Fig. 3). PC4 (about 3%) represented variation in temperature between winter and summer (Fig. 3).
Dendroclimatic relationships in the Aleppo pine tree-ring network
The basic statistics for 63 local chronologies are shown in Table 1. Mean correlation between trees and mean sensitivity of chronologies varied from 0.30 to 0.83 (average of 0.59) and from 0.18 to 0.65 (average of 0.34) respectively, indicating the potential for a common climate signal of varying strength among sites.
The CF analysis showed that ring-width index was primarily related to precipitation and secondarily to temperature. However, a high variability in the relationship between climate and growth was observed across the network (Fig. 4).
Only significant values are shown (p<0.05). Box plot showing the variability of correlation coefficients across the chronology network. The central horizontal lines indicate the median values, boxes enclose the central two quartiles, whiskers indicate the 10th and the 90th percentiles and the dots represent the full range of correlation coefficients. Dark and light grey areas indicate significance levels at 99 and 95%, respectively.
Tree-ring index was significantly related to annual total precipitation for all but two chronologies (FAR in France and AMA in Greece). The correlation of tree-ring index to winter temperature was usually significant and positive in colder locations, indicating reduced growth in years with cold winters. In contrast, at three network sites, where conditions are warmer, significant negative correlations indicated reduced growth in years with warm winters.
Variation in dendroclimatic relationships of Aleppo pine across its distribution
The significant regression equations of CF with the four PCs indicated that dendroclimatic relationships depended on the site position along the climatic gradient of the network (Table 2). This dependence produced a clear and coherent spatial pattern of tree growth in the Mediterranean Basin (Fig. 5). The CFs of annual temperature were inversely related to PC1 indicating that reduced growth associated with warm conditions occurred mainly in the southern part of the species distribution area where temperature was generally higher and precipitation lower than the regional average. Growth limitations due to cold conditions occurred in the northern part of the network where the average temperature was lower and precipitation less limiting than the regional average (Fig. 5). During winter, the area where cold conditions were associated with reduced tree growth was extended to the south. In contrast, in spring and summer reduced tree growth associated with warm conditions occurred in extended areas towards the southern distribution limits of Aleppo pine (Fig. 5).
Similarly, the inverse relationship of CF to PC2 suggests that the positive effects of annual, winter, and spring precipitation on tree growth significantly increased from wetter to drier sites. The influence of winter precipitation also increased along the wet to dry gradient being especially important at sites with high annual temperatures (Fig. 5). PC3 and PC4 were also significantly related to CFs suggesting that the variation in the seasonal distribution of precipitation and temperature across the network influenced the observed dendroclimatic relationships. PC3 was positively related with summer and negatively with winter precipitation while PC4 was positively related with sites with especially high summer temperatures.
Sensitivity of Aleppo pine to climate variations across its distribution area
The dendrochronological network of 63 sites comprises an essentially complete climatic gradient for Aleppo pine and provides information on how the growth of this species responds to climate variability across its distribution range. In the widely diverse set of climatic conditions, our study demonstrated that Aleppo pine is an excellent species for climate-growth studies. The mean correlation between trees in essentially all chronologies indicated that despite micro-environmental influences, a common pattern of year-to-year variation in ring width exists across all sites. The mean tree-ring sensitivity (the average relative difference in adjacent rings and a measure of climatic responsiveness) was generally higher than observed for other species in the Mediterranean area , , , , ,  highlighting the importance of Aleppo pine as a climate proxy for large regional areas. This could be especially important in the Mediterranean region where available climate observations are generally limited to the second half of the 20th century . Additional sampling sites at locations predicted to contain strong climatic signals based on these results would be especially useful to improve reconstruction of past climate. Distribution maps of climate-growth relationships across the distribution range of Aleppo pine may be also especially valuable to predict the response of trees to changing climate patterns.
High sensitivity of a species to climate variations across a wide geographic distribution area or in a wide range of climate conditions as observed here and in other dendrochronological studies suggests that climate change impacts may not be restricted to the ecotone or edge but may occur across the whole distribution range , . According to this, our results for Aleppo pine suggest that in the colder sites where low winter temperatures are presently limiting, future increased temperature may have a positive effect on tree growth. However, in the drier and warmer southern Mediterranean, where current positive anomalies in temperature have negative effects on tree growth, conditions for future tree growth will become less favorable as temperatures increase.
The role of phenotypic plasticity in a species response to climate variability under global warming
The ability of a species to respond through expression of existing genetic variation or phenotypic plasticity to changing environmental conditions may play a decisive role in species persistence or expansion under future global warming , . Phenotypic plasticity may be especially important as an adaptive strategy in trees and woody plants since the long generation time of many perennial species implies that the same set of genotypes needs to cope with year-to-year changing environmental conditions .
Generally, tree species are considered to express moderate to high plasticity in their responses to environmental stress , . Highly variable species that are able to survive in a broader range of environmental conditions are expected to better adjust to future climate conditions .
Pinus halepensis shows a wide ecological breadth and is adapted to a large range of environmental conditions, abiotic stressors, and perturbations . Several recent studies highlight substantial phenotypic plasticity of Pinus halepensis in relation to different anatomical, reproductive and vegetative traits , , , , , , , , , , , , , , , .
A substantial plasticity in the annual rhythms of cambial activity of Aleppo pine in response to different climatic conditions has been also well established. Thus, some studies suggest that the cambium is able to maintain activity throughout the whole year when and where climate conditions are favourable . Under other conditions, cambial activity can stop for one to three months during winter, depending on the prevalence of low temperatures . In addition, cambial activity in Pinus halepensis can slow down, or even stop, during summer drought , , , ,  and resume later when moisture availability increases in autumn , , , , , . In other circumstances, the trees can be subjected to “double stress” characterized by two stops in cambial activity - one during winter, caused by low temperatures, and one during the summer triggered by high temperatures and lack of precipitation , , , .
However, despite such evidence, plasticity in species responses to climate variability has been poorly explored from a dendrochronological perspective and variability in dendroclimatic response is more often considered as due to environmental noise in the tree-ring signal rather than a consequence of the plastic character of the species.
Geographical variation in climate conditions across the distribution area of a species creates differential selection pressures, and as a consequence population responses to climate variability are likely to vary among genetic provenances as well as through phenotypic plasticity. In this sense, our results highlight significant geographical variations in growth response of Aleppo pine to interannual climate variability suggesting substantial plasticity of growth in response to various climatic conditions. These results do not resolve the source of variability as genetic variation in provenance, phenotypic plasticity or some combination of these or other factors. However, the use of isoenzymes, terpene composition, and other markers has shown that Aleppo pine contains little genetic variability ,  and that intrapopulation genetic variability is generally much higher than interpopulation variability . These findings suggest that phenotypic plasticity has a dominant role in the observed variation in dendroclimatic response across distribution range of Aleppo pine. Then, as current growth responses to inter-annual climate variability vary spatially across existing climate gradients, we expect that future climate-growth relationships will also vary with position along future climate gradients.
Historical records of biological responses to climate in situ are perhaps the most dependable data we have to reconstruct past climate conditions, to predict future climate change impacts, and to develop realistic mitigation strategies. The tree-ring record is the most important and widely used source of long-term proxy data as they are available over a wide range of temporal and spatial scales.
However, the use of tree-rings to reconstruct past climate patterns and to forecast of future forest growth within the context of global warming is based on the uniformitarian principle (UP) that assumes that the climate-limiting factors that controlled tree-ring characteristics in the past continue the present and will extend into the future . Under UP, the modeled dendroclimatic relationship is viewed as stationary and consistent through time and the range of climatic variability.
To the contrary, our results show that dendroclimatic relationships significantly vary across species distribution in accordance to underlying climate conditions. Our results also suggest that such variations are likely to be related to the acclimation of trees to the new environmental conditions through a plastic response over decadal time periods. This complicates the simple projection of current relationships into the past or the future. For example, the response to winter temperature change from positive to negative moving from northern (mesic) to southern (drier) sites, with no response at intermediate sites along the thermal gradient. In this sense, the recent warming has a positive effect at the northern limit and a negative effect on the southern portion of the distribution range. At the same time, as summer drought severity increases the response to precipitation disappears, as the tree enters a quiescent and nonreactive state.
Accordingly, the application of UP to dendrochronology may be inappropriate when the dendroclimatic responses are likely to be inherently unstable and climate-dependent as we have shown for Aleppo pine in the Mediterranean basin.
Interestingly, a number of tree-ring studies have addressed changes in tree sensitivity and/or changes in the response of tree growth to climate in coincidence with unprecedented climate warming over the recent decades , , , , , , , . The causes of this lack of stability in the dendroclimatic relationship are still not well understood as all potential forcing factors (e.g., changes in climate, atmospheric CO2 concentration, or nitrogen deposition) capable of driving this change covary and obscure the individual impacts  but a plausible explanation could be the acclimation of trees to the new environmental conditions through a plastic response over decadal time periods. Our results are related to a limited geographical area and a specific dataset and may have limited application to other species or regions. However, such patterns may occur elsewhere and if so, it may have serious implications by affecting reliability of tree-ring based climate reconstructions.
In addition, climate model validation , species distribution models ,  and forecasts of future forest growth within the context of global warming , , , ,  whether or not based on dendrochronology also assumes a uniform response of species to climate variability and change without the competitive advantage conferred by genetic and/or phenotypic plasticity . These assumptions could lead to exaggerate or underestimate species at risk under future climate change.
Accurate prediction of growth of Aleppo pine and other species in response to future climate variability requires an understanding of the plasticity of the response of growth to climate variability and change. A more complex forest dynamics modeling approach considering genetic variation and phenotypic plasticity may contribute to resolve these uncertainties and would result in a more realistic characterization of the biological processes that govern species responses to climatic changes , , . In this sense, the reliability of old principles and assumptions requires re-examination and complementary theoretical and experimental frameworks should be developed to better understand what trees are telling us in a changing world.
The authors would like to thank the Academic editor Ben Bond-Lamberty and two anonymous reviewers that for their helpful and constructive comments on the earlier versions of the manuscript.
Conceived and designed the experiments: MdL. Performed the experiments: MdL KC ADF AP GP CBKR JR MAS KTS. Analyzed the data: MdL KN JR MAS. Contributed reagents/materials/analysis tools: MdL KC ADF AP GP CBKR JR MAS. Wrote the paper: MdL KTS.
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