Browse Subject Areas

Click through the PLOS taxonomy to find articles in your field.

For more information about PLOS Subject Areas, click here.

  • Loading metrics

Identification and Characterization of MicroRNAs in the Leaf of Ma Bamboo (Dendrocalamus latiflorus) by Deep Sequencing

  • Hansheng Zhao ,

    Contributed equally to this work with: Hansheng Zhao, Dongliang Chen

    Affiliation State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, International Center for Bamboo, Rattan, Beijing, China

  • Dongliang Chen ,

    Contributed equally to this work with: Hansheng Zhao, Dongliang Chen

    Affiliation State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, International Center for Bamboo, Rattan, Beijing, China

  • Zhenhua Peng , (ZMG); (ZHP)

    Affiliations State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, International Center for Bamboo, Rattan, Beijing, China, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, China

  • Lili Wang,

    Affiliation State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, International Center for Bamboo, Rattan, Beijing, China

  • Zhimin Gao (ZMG); (ZHP)

    Affiliation State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, International Center for Bamboo, Rattan, Beijing, China

Identification and Characterization of MicroRNAs in the Leaf of Ma Bamboo (Dendrocalamus latiflorus) by Deep Sequencing

  • Hansheng Zhao, 
  • Dongliang Chen, 
  • Zhenhua Peng, 
  • Lili Wang, 
  • Zhimin Gao


MicroRNAs (miRNAs), a class of non-coding small endogenous RNAs of approximately 22 nucleotides, regulate gene expression at the post-transcriptional levels by targeting mRNAs for degradation or by inhibiting protein translation. Thousands of miRNAs have been identified in many species. However, there is no information available concerning miRNAs in ma bamboo (Dendrocalamus latiflorus), one of the most important non-timber forest products, which has essential ecological roles in forests. To identify miRNAs in D. latiflorus, a small RNA library was constructed from leaf tissues. Using next generation high-throughput sequencing technology and bioinformatics analysis, we obtained 11,513,607 raw sequence reads and identified 84 conserved miRNAs (54 mature miRNAs and 30 star miRNAs) belonging to 17 families, and 81 novel miRNAs (76 mature miRNAs and five star miRNAs) in D. latiflorus. One hundred and sixty-two potential targets were identified for the 81 novel bamboo miRNAs. Several targets for the novel miRNAs are transcription factors that play important roles in plant development. Among the novel miRNAs, 30 were selected and their expression profiles in response to different light conditions were validated by qRT-PCR. This study provides the first large-scale cloning and characterization of miRNAs in D. latiflorus. Eighty-four conserved and 81 novel miRNAs were identified in D. latiflorus. Our results present a broad survey of bamboo miRNAs based on experimental and bioinformatics analysis. Although it will be necessary to validate the functions of miRNAs by further experimental research, these results represent a starting point for future research on D. latiflorus and related species.


MicroRNAs (miRNAs), initially discovered in Caenorhabditis elegans [1], are non-coding RNAs of approximately 22 nucleotides, which exist widely in animals, plants and some viruses [2-4]. MiRNAs have important functions in different developmental stages and metabolic process by mediating gene silencing at transcriptional and post-transcriptional levels [5-7]. In plants, miRNAs regulate leaf morphogenesis, the development of root and flowers and other key processes, such as response to biotic and abiotic stresses, via interactions with their specific target mRNAs [4,8-12].

With the development of next generation sequencing technologies, deep sequencing has become a valuable, powerful and effective high-throughput strategy for identifying novel miRNAs. To date, 21,264 entries representing hairpin precursor miRNAs, expressing 25,141 miRNAs, have been identified in 193 species; moreover, these data are available in the public miRNA database miRBase (Release 19, August 2012) [13]. Hundreds of miRNAs have been identified from Arabidopsis, Brassica rapa, barley, grapevine, maize, peanuts, rice, wheat, and other plants in previous research [14-25]. However, few studies have focused on the characterization of miRNAs in bamboo, which is one of the largest members of the grass subfamily Bambusoideae, of the family Poaceae (Gramineae) [26].

Bamboo, one of the most important non-timber forest products among the world’s plant and forest resources, is widely distributed in the tropical and subtropical areas under fluctuating light conditions. Most bamboos are fast growing, reaching their full height and diameter within a single growth season, which indicates that bamboos may possess unique carbon assimilation mechanisms in their leaves. D. latiflorus is an evergreen species locally known as ‘tropical giant bamboo’, which forms abundant forests in southern China and southeast Asia, and is a valuable natural resource used as food, building material and other human consumption [27]. Consequently, D. latiflorus is an obvious choice for an initial study of miRNAs in bamboo.

Using high-throughput sequencing and bioinformatics analysis, we identified 84 conserved miRNAs belonging to 17 families, and 81 novel miRNAs from more than 11 million raw sequence reads generated from a small RNA library of ma bamboo leaf. One hundred and sixty-two potential targets were identified for the 81 novel bamboo miRNAs. In addition, to confirm the novel predicted miRNAs in bamboo leaf tissues, the expression profiles of 30 novel miRNAs under different light conditions were validated by qRT-PCR. These results will lay the foundation for understanding miRNA-based regulation during D. latiflorus development.

Materials and Methods

Plant material, RNA isolation and small RNA high-throughput sequencing

Cutting seedlings of ma bamboo (D. latiflorus) were potted in our laboratory under a regime of 16 h light and 8 h darkness at 25°C, with a light intensity of 200 μmol·m-2·s-1 and a relative humidity of 75%. As experimental materials, we chose the third piece of new functional leaf (blade tissue only) from the top of the branch, which could be considered a juvenile leaf. The leaves were collected from 2-year-old cuttings and quickly frozen in liquid nitrogen. Total RNA was isolated from leaf tissues using the Trizol reagent (Invitrogen, Carlsbad, CA, USA), according to the manufacturer’s instructions.

The small RNA library construction for ma bamboo and Solexa sequencing were carried out at BGI-Shenzhen (Shenzhen, China) using the standard Solexa protocol [28]. Briefly, small RNAs of 15–30 nt in length were first isolated from the total RNA through 15% TBE urea denaturing polyacrylamide gels. Subsequently, 5′ and 3′ RNA adaptors were ligated to these small RNAs, followed by reverse transcription into cDNAs. These cDNAs were amplified by PCR and subjected to Solexa sequencing. After removing low quality reads and trimming adapter sequences, small RNAs ranging from 18–30 nt were collected and used for further analyses. Finally, the selected clean reads were analyzed by BLAST against the Rfam database ( [29] and the GenBank non-coding RNA database ( to discard rRNA, tRNA, snRNA and other ncRNA sequences. In addition, sequencing data within this study were firstly uploaded to NIH Short Read Archive (accession number: SRX347876).

Data content

To obtain and analyze miRNAs from the leaf of ma bamboo, three types of data were used in this study (1). MiRNA data. The miRNA data from the leaf of ma bamboo was obtained by next generation sequencing, and all the reference sequences of mature miRNAs, star miRNAs and their precursors (pre-miRNAs) were downloaded from miRBase, Release 19.0 ( [13]. (2) Genome data of moso bamboo (Phyllostachys heterocycla var. pubescens) [30]. Although the whole genome sequence of ma bamboo is not available, phylogenomic analyses suggested that ma bamboo and moso bamboo had the closest relationship, with high sequence similarity [31]. For this reason, the sequenced genome of moso bamboo from our previous study was used as the genome to analyze ma bamboo miRNA data (3). Expressed sequence tag (EST) and mRNA data from ma bamboo. The approach outlined in (2) would inevitably produce some biases and some miRNAs would not be identified. Therefore, to fill the gap, ESTs and other mRNA data from ma bamboo were obtained from the NCBI database site and used to identify additional miRNAs.

Prediction of conserved miRNAs, novel miRNAs and potential miRNA targets in ma bamboo

As miRNA precursors have a characteristic fold-back structure, 150 nt of the sequence flanking the genomic sequences of small RNAs (sRNAs) was extracted and used to predict miRNAs. For analysis of conserved miRNAs in ma bamboo, unique sRNAs were aligned with plant mature miRNAs in miRBase Release 19.0. First, after rigorous screening, all retained sequences with three or more copies were considered as potential miRNAs. Second, sRNA sequences with no more than four mismatched bases were selected by BLAST searching against miRBase. Third, the remaining 15–26 nt reads were used to map the genome of moso bamboo and ESTs of ma bamboo using the BLASTN program. Sequences with a tolerance of two mismatches were retained for miRNA prediction. RNAfold ( [32] was used for secondary structure prediction (hairpin prediction) of individual mapped miRNAs, using the default folding conditions to identify the known conserved miRNAs in ma bamboo. Finally, sequences that were not identical to the conserved miRNAs were termed novel miRNAs.

Potential target sequences for the newly identified miRNAs were predicted using the psRNATarget program ( with default parameters. Newly identified miRNA sequences for ma bamboo were used as custom miRNA sequences, while coding sequences for moso bamboo, as well as EST and mRNA databases for ma bamboo, were used as custom plant databases, respectively. All predicted target genes were evaluated by the scoring system and criteria defined in a previous report [33]. Sequences with a total score less than 3.0 were identified as miRNA targets.

Expression analysis of novel miRNAs by qRT-PCR

The stem-loop qRT-PCR method [3] was used to detect the expression levels of novel miRNAs in D. latiflorus. Forward primers were specifically designed for each individual miRNA, as detailed in a previous method [3]: six nucleotides of the 3' end of the stem-loop RT primer were complementary to the 3' end of the mature miRNA, and the sequence 5' - CTCAACTGGTGTCGTGGAGTC - 3' was used as the universal reverse primer [34]. U6 snRNA was used as an internal control [35]. More detailed information is supplied in File S1.

Total RNA was isolated from leaves under three different light conditions, namely CK (the control of 200 μmol•m-2•s-1 for 4 h), high light (1200 μmol•m-2•s-1 for 4 h) and dark (dark for 24 h). cDNAs were synthesized from total RNA with the miRNA-specific stem-loop RT primer. The volume of the reverse transcription reaction was 15 μL, which contained 300–500 ng total RNA, 1 μL (2 μM) of RT primer, 3 μL of 5 × reaction buffer, 1.3 μL (each 10 mM) dNTP mix, 1 μL (40 U/μL) RNAsafe and 0.5 μL (200 U/μL) M-MLV reverse transcriptase. The reaction was incubated at 16°C for 30 min, 42°C for 30 min, 85°C for 5 min and 4°C for 5 min.

The qRT-PCR was conducted using a SYBR Green I Master Kit (Roche, Germany) on a LightCycler® 480 Real-Time PCR System (Roche). The final volume was 20 μl, containing 7.5 μl 2×SYBR Premix Ex Taq, 0.3 μl of each primer (10 μM), 2 μl of cDNA and 7.2 μl of nuclease-free water. The amplification was carried out as follows: initial denaturation at 95°C for 10 min, followed by 41 cycles at 95°C for 10 s, 55°C for 20 s, and 72°C for 10 s. The melting curves were adjusted as 95°C for 5 s and 55°C for 1 min and then cooled to 40°C for 30 s [36]. All reactions were repeated three times.

For each condition, the qRT-PCR experiments were performed as biological triplicates and expression levels were normalized according to that of the internal control. The relative value of the gene expression was calculated using the 2-ΔΔCt method [37]. Statistical tests were performed on the qRT-PCR data using SPSS (Statistical Product and Service Solutions) 18.0 software. Error bars representing the standard deviation were derived from the three experiments in triplicate.

Results and Discussion

Overview of small RNA library sequencing

Deep sequencing of the small RNA library from D. latiflorus leaf tissues produced 11,513,607 raw sequence reads. Low quality sequences, adapters and small sequences shorter than 16 nucleotides (nt) were removed, leaving 10,593,305 clean reads and 6,320,379 unique sequences. After further removal of unannotated small RNAs and non-coding RNAs, such as tRNAs, rRNAs, siRNAs, snRNAs, snoRNAs and other non-coding RNAs, 910,151 miRNA sequences, accounting for 8.59% of the total sRNA, were identified (Table 1).

CategoryUnique sRNAPercent (%)Total sRNAPercent (%)

Table 1. Summary of small RNA in sequencing data.

Download CSV

The significant feature of the size profile permitted miRNAs to be distinguished from other small RNAs. The sRNAs length distribution (10–30 nt) of the original reads demonstrated that the most abundant reads were those of 20–24 nt in length (Figure 1), which were consistent with sRNAs with known function [19]. The most abundant class was those of 24 nt, which was also highly consistent with those small RNA of other Poaceae plants based on Solexa sequencing technology, such as rice [38], barley [39] and wheat [40].

Figure 1. Length, distribution and abundance of small RNAs in D. latiflorus.

Identification of conserved miRNAs in ma bamboo

Conserved miRNAs have been found in many plant species and play significant roles in plant development and stress responses [4]. To identify the conserved miRNAs in D. latiflorus, the sRNA library was searched using BLASTN for unique mature plant miRNA sequences in miRBase Version 19.0, in which miRNAs of bamboo had not been identified. Following a set of strict filtering criteria and further sequence analysis, 54 conserved miRNAs and 30 conserved star miRNAs were identified from D. latiflorus, which belonged to 17 families (Table 2).

miRNA familyNameNovel mature miRNAPrecursor
SequenceRead countLength (nt)SequenceRead countLength (nt)

Table 2. Conserved miRNAs in D. latiflorus.

Download CSV

Compared with the conserved miRNA families in plants reported by Cuperus et al [41], 15 conserved miRNA families, accounting for 88% of the conserved miRNA families in ma bamboo, were represented in ma bamboo. However, of the 29 conserved miRNA families in ehrhartoideae [41], 14 conserved miRNA families were not represented in ma bamboo. This may have resulted from the data in miRBase database being a different version, may represent the particular evolution and function of miRNAs in ma bamboo, or may have resulted from the inevitable biases in processes such as the preparation of samples, sequencing and analysis. Further experiments using increased amounts of data are required to verify the conserved miRNA families in ma bamboo. In addition, based on the moso bamboo genome, 13 conserved miRNA families were predicted and compared with those experimentally derived from ma bamboo. This analysis indicated that eight conserved miRNA families, accounting for 62% of all families predicted in moso bamboo, were represented in ma bamboo. This may be explained by the fact that they are two different bamboo species with distinct biological and evolutionary features. For example, the number of chromosomes is 68 and 48 in ma bamboo and moso bamboo, respectively.

MiRNAs with high sequencing frequencies have been shown to play fundamental and essential regulatory functions in maintaining biological processes. Therefore, the read counts for known miRNA families were analyzed (Figure 2). The most abundant miRNA family (594,744 reads) was miR168, which was represented five to 41 times more frequently than other relatively high abundance miRNAs, including miR156/157, miR535, miR165/166 and miR167, whose total abundances ranged from 14,448 to 109,638 reads. Moreover, the top three most abundant miRNA family members (miR168, miR156/157 and miR535) were represented by 594,744, 109,642 and 77,838 reads, respectively, accounting for ~93.6% of the expressed miRNA tags. However, the lowest abundant miRNA families (miR528, miR319 and miR1878) represented only ~0.003% of expressed miRNA tags, indicating that these miRNAs were expressed at a low level.

The number of members in the differently conserved miRNA families was also analyzed. Four families (miR156/157, miR165, miR167 and miR535) contained multiple members, with nine, six, five and six members respectively. Five families, miR390, miR396, miR399, miR827 and miR1878, only had one member.

The bamboo flowering cycle can take up to 120 years and also involves infrequent and unpredictable flowering events, as well as peculiar monocarpic behavior, e.g., flowering once before culm death [26]; therefore, the mechanism of flowering, as a unique characteristic of bamboo, has received much research interest. Studies in other plants indicated that miRNAs play important roles in flower development [42,43]. The transition from juvenile to adult phase is controlled by miR156 and miR172. MiR172 also influences floral organ identity, as evidenced by failure of carpel abortion from the male inflorescence [42]. In addition, extensive analysis of certain monocotyledonous species, such as Brachypodium distachyon, Oryza sativa, Sorghum bicolor, and Zea mays, revealed more than three members in each miR172 family. Therefore, absence of miR172 may contribute to bamboo’s special regulatory mechanism of flower development. Other miRNAs (miR164, miR167, miR169 and miR319) with functions during different stages of flower development were detected in D. latiflorus.

As seen in Figure 3, to explore the evolutionary roles of these conserved miRNAs, deep analyses focused on extensive comparisons against known conserved miRNAs in other plant species, including Picea abies, Pinus taeda, Physcomitrella patens, Selaginella moellendorffii, Arabidopsis thaliana, Brassica napus, Ricinus communis, Medicago truncatula, Citrus sinensis, Vitis vinifera, O. sativa, S. bicolor and Z. mays. Based on BLAST searches and sequences analysis, some miRNA families of D. latiflorus (miR156/157, miR160, miR165/166, miR171, miR319, miR390 and miR396) are highly conserved and ancient. For example, miR156/157 is a family of endogenous miRNAs with a relatively high expression level in the juvenile phase of many plants. The level of miR156/157 gradually decreases with plant age [44,45].

Figure 3. Conserved miRNAs from D. latiflorus and their homologs in other some species.

Scale 0: miRNA undetected; Scale 1: miRNA identified using computational programs; Scale 2: miRNA sequenced using experimental cloned or high-throught technology; Scale 3: miRNA/miRNA* accumulation detected; Scale 4: miRNA detected by small RNA blot or RT-PCR or Northern; Scale 5: miRNAs with validated targets. Total information in Figure 3 is from miRBase 19.0 and references 14,47,63-84. Abbreviation: ath, Arabidopsis thaliana; bna, Brassica napus; rco, Ricinus communis; mtr, Medicago truncatula; csi, Citrus sinensis; vvi, Vitis vinifera; osa, Oryza sativa; sbi, Sorghum bicolor; zma, Zea mays; pab, Picea abies; pta, Pinus taeda; ppt, Physcomitrella patens; smo, Selaginella moellendorffii.

However, some miRNA families (miR528, miR535, miR827, miR1318 and miR1878) are less evolutionarily conserved and are involved in regulation of diverse physiological processes. For example, the miR528-target recognition site is only present within monocot genes, while all eudicot genes orthologous to SsCBP1 from Arabidopsis, poplar, grape, and soybean genomes completely lack the miR528-target recognition site, which supports the view that miR528 is a monocot-specific miRNA [46]. Another example is miR535, which was predicted to target a gene encoding a brassinosteroid signaling positive regulator protein in Physocmitrella patens [47]. It was reported that mature miR535 may be considered as a divergent member of the miR156/157 family, and targeting of SPL genes may be a vestigial function of miR535, which may have been performed more efficiently in the course of evolution by other members of the family [48].

The remaining five miRNA families, including miR164, miR167, miR168, miR169 and miR399, are homologous in eudicotyledons and monocotyledons, indicating these miRNA families may be recent and are more sensitive to certain stresses. For example, miR168 regulates loop ARGONAUTE1 (AGO1) homeostasis, which is crucial for the regulation of gene expression and plant development [49]. Another report demonstrated that NFYA5 contained a target site of miR169, which was downregulated by drought stress through an ABA-dependent pathway [50,51]. MiR399 is upregulated in phosphate starvation and its target gene, encoding a ubiquitin-conjugating E2 enzyme, is downregulated in A. thaliana [50,51].

Identification of novel miRNAs in ma bamboo

Previous studies have shown that each species has species-specific miRNAs [17,18,24,25,33,39,52-54]; therefore, ma bamboo is also likely to have unique miRNAs. In addition, a distinct feature of miRNAs is the ability of their pre-miRNA sequences to adopt the canonical stem-loop hairpin structure. After excluding sRNA reads homologous to known miRNAs and other non-coding sRNAs, the remaining 18-24 nt sRNAs were subjected to secondary structure analysis of their precursors using RNA fold software. Seventy-six novel miRNAs and five novel star miRNAs were identified as possible miRNA candidates in ma bamboo (Table 3). ESTs of ma bamboo were used to predict miRNAs; however, no novel miRNAs were detected, which might be explained by the small number of ESTs.

NameNovel mature miRNAPrecursor
SequenceRead countLength (nt)Precursor IDPositionStandMFE (kcal/mol)

Table 3. Novel miRNAs in D. latiflorus.

Download CSV

Precursors of these novel miRNAs were identified and formed proper secondary hairpin structures, with minimal folding free energy (MFE) ranging from -20.3 kcal·mol-1 to -46.1 kcal·mol-1 (average of ~ -31.0 kcal·mol-1).

Moreover, the length distributions of plant pre-miRNAs are more heterogeneous than animal pre-miRNAs. The shortest pre-miRNA is 53 nt in length (miRBase ID: ath-MIR5645b) and the longest in 938 nt in length (miRBase ID: aly-MIR858) in miRBASE Version 19. In ma bamboo, the distribution of pre-miRNAs is 54-96 nt, which is consistent with the pre-miRNAs distributions in other plants [17,25,39]. In addition, the length distribution of novel miRNA ranged from 18–24 nt in length. Forty-eight (45.3%) of the novel miRNAs belong to the 24-nucleotide class, representing the most abundant novel miRNAs.

Among the novel miRNAs, dla-miRC1 had the highest expression in our data, with 4,801 reads. Based on their frequencies and sequences in the small RNA library, although the expression levels of these candidates ranged from thousands of reads to single reads, in general, novel miRNA candidates of D. latiflorus showed lower expression compared with most of the conserved families. The low abundance of novel miRNAs might indicate that these miRNAs play a specific role in certain tissues or developmental stages, and may be considered young miRNAs in terms of evolution [41]. This sRNA library was only generated from leaf tissues, and future experiments will be carried out to determine whether these low-abundant miRNAs are expressed at higher levels in other organs, such as flowers and seeds, or whether they are regulated by certain stresses.

Prediction of miRNA targets in ma bamboo

The identification of miRNA targets using bioinformatics approaches is an essential step to further understand the regulatory function of miRNAs [25,33,55]. Given the lack of genomic data for ma bamboo, the CDS from the genome of the closely related species moso bamboo and ESTs of ma bamboo were used to predict new miRNA target genes, using the criteria described in the material and methods. We identified 176 potential targets, including 139 targets from moso bamboo in File S2 and 37 targets from ma bamboo (Table 4).

miRNA nameTarget IDInhibitionTarget annotation
dla-miRC1JK012907.1Cleavageauxin response factor 15
JK010246.1Cleavageauxin response factor
dla-miRC2JK012063.1Cleavagephospholipase D
dla-miRC4JK015107.1Cleavagephosphoribosyl transferase
dla-miRC5JK010514.1TranslationR3H domain containing protein
JK015560.1TranslationBRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 precursor
JK008104.1CleavageNLI interacting factor-like phosphatase
JK016203.1Translationsialyltransferase family domain containing protein
JK008046.1Cleavageexpressed protein
JK016171.1Translationgibberellin response modulator protein
JK016234.1Cleavageexpressed protein
JK011714.1Translationethylene-responsive protein related
JK012545.1Translationexpressed protein
JK011067.1Translationglucan endo-1,3-beta-glucosidase-related
JK015476.1TranslationLIM domain-containing protein
JK008460.1Cleavagetransporter,major facilitator family
dla-miRC15JK016083.1TranslationCyclopropane-fatty-acyl-phospholipid synthase
dla-miRC27-5pJK015342.1Translationprotein phosphatase 2C containing protein
dla-miRC31JK014807.1Translationprotein kinase family protein
dla-miRC33JK009785.1CleavageATP synthase B chain, chloroplast precursor
JK013093.1CleavageRNA recognition motif containing protein
JK008661.1Cleavageexpressed protein
dla-miRC35JK010448.1CleavageCBS domain containing membrane protein
JK012636.1CleavageCBS domain containing membrane protein
JK014547.1CleavageCBS domain containing membrane protein
JK016367.1CleavageCBS domain containing membrane protein
JK016562.1CleavageCBS domain containing membrane protein
JK011948.1CleavageRNA polymerase sigma factor
JK012079.1CleavageRNA polymerase sigma factor
JK012720.1CleavageRNA polymerase sigma factor
JK013456.1CleavageRNA polymerase sigma factor
JK012868.1CleavageRNA polymerase sigma factor
JK016358.1CleavageRNA polymerase sigma factor
JK014679.1CleavageRNA polymerase sigma factor
dla-miRC50JK012577.1Translationexpressed protein

Table 4. Potential targets gene for novel miRNAs in D. latiflorus based on EST data of D. latiflorus.

Download CSV

Among the novel miRNAs, dla-miRC5 has 26 putative target genes with different functions, which indicates that dla-miRC5 might be involved in regulating the expression of multiple genes in ma bamboo. Some of the novel miRNAs target transcription factor (TF) genes that have been confirmed to play key roles in plant development. The targets of dla-miRC1 are auxin response factor (ARF) TF genes. ARF can regulate auxin response genes by binding specifically to cis-elements of their promoters to affect the developmental process of plants [55]. To date, miR160 and miR167 have been demonstrated to inhibit ARF10, ARF16, ARF17 [8,56] and ARF6, ARF8 [57], respectively; however, dla-miRC1 is barely homologous to miR160 and miR167, indicating that it might be another specific suppressor of ARF genes in ma bamboo.

In addition, an ERF gene (belonging to AP2/EREBP family) is predicted to be a target of dla-miRC37, a MYB TF gene is a putative target of dla-miRC56, and both dla-miRC9 and dla-miRC16 have one target gene belonging to the BHLH family of TFs. Scarecrow, of the GRAS TF gene family, is targeted by dla-miRC33. All the targets of these novel miRNAs were TF genes that function in regulating the development of plants [58-61], which indicated these miRNAs might play important roles in ma bamboo development and stress responses.

Some novel miRNAs, including dla-miRC2, dla-miRC5, dla-miRC13, dla-miRC35 and dla-miC45, are predicted to target genes related to chloroplast synthesis and photosynthesis in the leaf, which is the most important photosynthetic organ of plants. This result suggested that the miRNAs might be leaf-specific and be involved in the process of regulating chloroplast synthesis and photosynthesis.

Expression profiles of novel miRNAs in response to light

The expression patterns of miRNAs could provide clues to their functions [22]. As an efficient and sensitive method for detecting gene expression [62], stem-loop qRT-PCR was developed based on the common qRT-PCR method, with advantages such as increased sensitivity and high accuracy. Therefore, stem-loop qRT-PCR has been widely employed to distinguish two miRNAs with small differences [3].

Among the novel predicted miRNAs, the top 30 novel miRNAs (according to the number of reads) were selected and primers were designed based on their highly specific stem-loop reverse sequences to experimentally identify their expression. As shown in File S3, the result of stem-loop qRT-PCR showed that each of the selected miRNAs had a highly specific dissolution curve, which indicated that the primers could be used for further analysis. Moreover, as shown in Figure 4, the in-depth analysis demonstrated the expression of the miRNAs under high light stress conditions; the number of miRNAs that were upregulated and downregulated was 10 and 16, respectively. The expressions of dla-miRC18, dla-miRC27-5p and dla-miRC27-3p were significantly increased under high light (P<0.01), among which dla-miRC18 was upregulated to 15 times the level of the control. However, the expression of dla-miRC1, dla-miRC19 and dla-miRC28 was significantly downregulated (P<0.01). Under dark condition, the expressions of dla-miRC1, dla-miRC22, dla-miRC25, dla-miRC27-5p and dla-miR27-3p were upregulated significantly (P<0.01), while the expressions of dla-miRC5, dla-miRC17 and dla-miRC29 were downregulated significantly (P<0.01).

Figure 4. Expression profiles of novel miRNAs in leaves of D. latiflorus response to different light conditions.

CK: 200 µmol·m-2·s-1 for 4 h; High light: 1200 µmol·m-2·s-1 for 4 h; Dark: dark for 24 h.

This analysis also indicated that the expressions of dla-miRC1 and dla-miRC19 were downregulated significantly under high light and upregulated markedly under dark conditions (P<0.01), indicating that they were inhibited by light. However, dla-miRC29 was upregulated under high light and downregulated under dark, indicating it was light-induced expression. MiR27-5p and miR27-3p were cleaved from the same precursor and had similar expression profiles, being upregulated under both high light and dark, which indicated they might have the same promoter and cis-elements, and synergistic expression characteristics. The expression results indicated that these miRNAs were affected by light, and might play vital roles in the regulation of genes involved in light signal transduction and light stress.

Bamboo converts light energy into chemical energy through photosynthesis, which is one of the necessary processes supplying carbohydrates for the rapid expansion of cells. As the first comparative identification of miRNAs among leaves treated with distinct light conditions, this analysis showed that bamboo may possess a unique light regulation mechanism involving miRNAs, although its function and mechanism are currently unknown.


We identified 81 novel miRNAs and 84 conserved miRNAs belonging to 17 families in the leaf of ma bamboo using high-throughput sequencing and bioinformatics analysis. The results of qRT-PCR indicated the miRNAs might regulate the expression of genes involved in photosynthesis, which acts as a key metabolic pathway in the fast growth of bamboo. These miRNAs will add to the growing database of new miRNAs and lay the foundation for further understanding of miRNA function in the regulation of ma bamboo development and other biological characteristics. These miRNAs identified in the leaf of ma bamboo provide new opportunities for future functional genome research in bamboo and other related species.

Supporting Information

File S1.

Primers used in novel miRNAs qRT-PCR.


File S2.

Potential targets gene for novel miRNAs in D. latiflorus based on moso bamboo CDS data.


File S3.

Dissolution curves of U6 snRNA and 30 novel miRNAs.



We thank the reviewer for the positive and constructive comments on drafts of the manuscript.

Author Contributions

Conceived and designed the experiments: ZMG. Performed the experiments: ZMG DLC. Analyzed the data: HSZ LLW. Contributed reagents/materials/analysis tools: HSZ ZHP. Wrote the manuscript: HSZ ZMG.


  1. 1. Lee RC, Feinbaum RL, Ambros V (1993) The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell 75: 843-854. doi:10.1016/0092-8674(93)90529-Y. PubMed: 8252621.
  2. 2. Bushati N, Cohen SM (2007) microRNA functions. Annu Rev Cell Dev Biol 23: 175-205. doi:10.1146/annurev.cellbio.23.090506.123406. PubMed: 17506695.
  3. 3. Chen C, Ridzon DA, Broomer AJ, Zhou Z, Lee DH et al. (2005) Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Res 33: e179. doi:10.1093/nar/gni178. PubMed: 16314309.
  4. 4. Jones-Rhoades MW, Bartel DP, Bartel B (2006) MicroRNAs and their regulatory roles in plants. Annu Rev Plant Biol 57: 19-53. doi:10.1146/annurev.arplant.57.032905.105218. PubMed: 16669754.
  5. 5. Aukerman MJ, Sakai H (2003) Regulation of flowering time and floral organ identity by a MicroRNA and its APETALA2-like target genes. Plant Cell 15: 2730-2741. doi:10.1105/tpc.016238. PubMed: 14555699.
  6. 6. Bari R, Datt Pant B, Stitt M, Scheible WR (2006) PHO2, microRNA399, and PHR1 define a phosphate-signaling pathway in plants. Plant Physiol 141: 988-999. doi:10.1104/pp.106.079707. PubMed: 16679424.
  7. 7. Llave C, Xie Z, Kasschau KD, Carrington JC (2002) Cleavage of Scarecrow-like mRNA targets directed by a class of Arabidopsis miRNA. Science 297: 2053-2056. doi:10.1126/science.1076311. PubMed: 12242443.
  8. 8. Wang JW, Wang LJ, Mao YB, Cai WJ, Xue HW et al. (2005) Control of root cap formation by MicroRNA-targeted auxin response factors in Arabidopsis. Plant Cell 17: 2204-2216. doi:10.1105/tpc.105.033076. PubMed: 16006581.
  9. 9. Palatnik JF, Allen E, Wu X, Schommer C, Schwab R et al. (2003) Control of leaf morphogenesis by microRNAs. Nature 425: 257-263. doi:10.1038/nature01958. PubMed: 12931144.
  10. 10. Kidner CA, Martienssen RA (2005) The developmental role of microRNA in plants. Curr Opin Plant Biol 8: 38-44. doi:10.1016/j.pbi.2004.11.008. PubMed: 15653398.
  11. 11. Guo HS, Xie Q, Fei JF, Chua NH (2005) MicroRNA directs mRNA cleavage of the transcription factor NAC1 to downregulate auxin signals for Arabidopsis lateral root development. Plant Cell 17: 1376-1386. doi:10.1105/tpc.105.030841. PubMed: 15829603.
  12. 12. Chen X (2004) A microRNA as a translational repressor of APETALA2 in Arabidopsis flower development. Sci Signal 303: 2022.
  13. 13. Kozomara A, Griffiths-Jones S (2011) miRBase: integrating microRNA annotation and deep-sequencing data. Nucleic Acids Res 39: D152-D157. doi:10.1093/nar/gkr817. PubMed: 21037258.
  14. 14. Rajagopalan R, Vaucheret H, Trejo J, Bartel DP (2006) A diverse and evolutionarily fluid set of microRNAs in Arabidopsis thaliana. Genes Dev 20: 3407-3425. doi:10.1101/gad.1476406. PubMed: 17182867.
  15. 15. Peláez P, Trejo MS, Iñiguez LP, Estrada-Navarrete G, Covarrubias AA et al. (2012) Identification and characterization of microRNAs in Phaseolus vulgaris by high-throughput sequencing. BMC Genomics 13: 83. doi:10.1186/1471-2164-13-83. PubMed: 22394504.
  16. 16. Yu X, Wang H, Lu Y, de Ruiter M, Cariaso M et al. (2012) Identification of conserved and novel microRNAs that are responsive to heat stress in Brassica rapa. J Exp Bot 63: 1025-1038. doi:10.1093/jxb/err337. PubMed: 22025521.
  17. 17. Zhao CZ, Xia H, Frazier TP, Yao YY, Bi YP et al. (2010) Deep sequencing identifies novel and conserved microRNAs in peanuts (Arachis hypogaea. p. L.). BMC Plant Biol 10: 3.
  18. 18. Lertpanyasampatha M, Gao L, Kongsawadworakul P, Viboonjun U, Chrestin H et al. (2012) Genome-wide analysis of microRNAs in rubber tree (Hevea brasiliensis L.) using high-throughput sequencing. Planta, 236: 1-9. PubMed: 22407387.
  19. 19. Pantaleo V, Szittya G, Moxon S, Miozzi L, Moulton V et al. (2010) Identification of grapevine microRNAs and their targets using high-throughput sequencing and degradome analysis. Plant J 62: 960-976. PubMed: 20230504.
  20. 20. Sunkar R, Zhou X, Zheng Y, Zhang W, Zhu JK (2008) Identification of novel and candidate miRNAs in rice by high throughput sequencing. BMC Plant Biol 8: 25. doi:10.1186/1471-2229-8-25. PubMed: 18312648.
  21. 21. Zhang J, Xu Y, Huan Q, Chong K (2009) Deep sequencing of Brachypodium small RNAs at the global genome level identifies microRNAs involved in cold stress response. BMC Genomics 10: 449. doi:10.1186/1471-2164-10-449. PubMed: 19772667.
  22. 22. Yao Y, Guo G, Ni Z, Sunkar R, Du J et al. (2007) Cloning and characterization of microRNAs from wheat (Triticum aestivum. p. L.). Genome Biol 8: R96.
  23. 23. Mica E, Piccolo V, Delledonne M, Ferrarini A, Pezzotti M et al. (2009) High throughput approaches reveal splicing of primary microRNA transcripts and tissue specific expression of mature microRNAs in Vitis vinifera. BMC Genomics 10: 558. doi:10.1186/1471-2164-10-558. PubMed: 19939267.
  24. 24. Schreiber AW, Shi B-J, Huang C-Y, Langridge P, Baumann U (2011) Discovery of barley miRNAs through deep sequencing of short reads. BMC Genomics 12: 129. doi:10.1186/1471-2164-12-129. PubMed: 21352554.
  25. 25. Song QX, Liu YF, Hu XY, Zhang WK, Ma B et al. (2011) Identification of miRNAs and their target genes in developing soybean seeds by deep sequencing. BMC Plant Biol 11: 5. doi:10.1186/1471-2229-11-5. PubMed: 21219599.
  26. 26. Singh SR, Singh R, Kalia S, Dalal S, Dhawan AK et al. (2013) Limitations, progress and prospects of application of biotechnological tools in improvement of bamboo—a plant with extraordinary qualities. Physiol Mol Biol Plants 19: 21-41. doi:10.1007/s12298-012-0147-1.
  27. 27. Lin CS, [!(surname)!] , Hsaio HW, Lin MJ, Chang WC (2007) Flowering of the green and albino regenerates obtained via somatic embryogenesis in inflorescence segments of Dendrocalamus latiflorus. New Forests: 9.
  28. 28. Thomas MF, Ansel KM (2010) Construction of small RNA cDNA libraries for deep sequencing. Methods Mol Biol 667: 93-111. doi:10.1007/978-1-60761-811-9_7. PubMed: 20827529.
  29. 29. Burge SW, Daub J, Eberhardt R, Tate J, Barquist L et al. (2013) Rfam. p. 11.0: 10 years of RNA families. Nucleic Acids Res 41: D226-232.
  30. 30. Peng Z, Lu Y, Li L, Zhao Q, Feng Q et al. (2013) The draft genome of the fast-growing non-timber forest species moso bamboo (Phyllostachys heterocycla). Nat Genet 45: 456-461, 461e451-452 doi:10.1038/ng.2569. PubMed: 23435089.
  31. 31. Zhao L, Zhang N, Ma PF, Liu Q, Li DZ et al. (2013) Phylogenomic analyses of nuclear genes reveal the evolutionary relationships within the BEP clade and the evidence of positive selection in poaceae. PLOS ONE 8: e64642. doi:10.1371/journal.pone.0064642. PubMed: 23734211.
  32. 32. Denman RB (1993) Using RNAFOLD to predict the activity of small catalytic RNAs. BioTechniques 15: 1090-1095. PubMed: 8292343.
  33. 33. Chi X, Yang Q, Chen X, Wang J, Pan L et al. (2011) Identification and characterization of microRNAs from peanut (Arachis hypogaea L.) by high-throughput sequencing. PLOS ONE 6: e27530. doi:10.1371/journal.pone.0027530. PubMed: 22110666.
  34. 34. Varkonyi-Gasic E, Wu R, Wood M, Walton EF, Hellens RP (2007) Protocol: a highly sensitive RT-PCR method for detection and quantification of microRNAs. Plants Methods 3: 12. doi:10.1186/1746-4811-3-12. PubMed: 17931426.
  35. 35. Ding Y, Chen Z, Zhu C (2011) Microarray-based analysis of cadmium-responsive microRNAs in rice (Oryza sativa). J Exp Bot 62: 3563-3573. doi:10.1093/jxb/err046. PubMed: 21362738.
  36. 36. Unver T, Budak H (2009) Conserved microRNAs and their targets in model grass species Brachypodium distachyon. Planta 230: 659-669. doi:10.1007/s00425-009-0974-7. PubMed: 19585143.
  37. 37. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 25: 402-408. doi:10.1006/meth.2001.1262. PubMed: 11846609.
  38. 38. Yi R, Zhu Z, Hu J, Qian Q, Dai J et al. (2013) Identification and Expression Analysis of microRNAs at the Grain Filling Stage in Rice(Oryza sativa L.)via Deep Sequencing. PLOS ONE 8: e57863. doi:10.1371/journal.pone.0057863. PubMed: 23469249.
  39. 39. Lv S, Nie X, Wang L, Du X, Biradar SS et al. (2012) Identification and Characterization of MicroRNAs from Barley (Hordeum vulgare L.) by High-Throughput Sequencing. Int J Mol Sci 13: 2973-2984. doi:10.3390/ijms13032973. PubMed: 22489137.
  40. 40. Li YF, Zheng Y, Jagadeeswaran G, Sunkar R (2013) Characterization of small RNAs and their target genes in wheat seedlings using sequencing-based approaches. Plant Sci 203-204: 17-24. doi:10.1016/j.plantsci.2012.12.014. PubMed: 23415324.
  41. 41. Cuperus JT, Fahlgren N, Carrington JC (2011) Evolution and functional diversification of MIRNA genes. Plant Cell 23: 431-442. doi:10.1105/tpc.110.082784. PubMed: 21317375.
  42. 42. Nag A, Jack T (2010) Sculpting the flower; the role of microRNAs in flower development. Curr Top Dev Biol 91: 349-378. doi:10.1016/S0070-2153(10)91012-0. PubMed: 20705188.
  43. 43. Wollmann H, Weigel D (2010) Small RNAs in flower development. Eur J Cell Biol 89: 250-257. doi:10.1016/j.ejcb.2009.11.004. PubMed: 20031253.
  44. 44. Matts J, Jagadeeswaran G, Roe BA, Sunkar R (2010) Identification of microRNAs and their targets in switchgrass, a model biofuel plant species. J Plant Physiol 167: 896-904. doi:10.1016/j.jplph.2010.02.001. PubMed: 20207044.
  45. 45. Wu G, Park MY, Conway SR, Wang JW, Weigel D et al. (2009) The sequential action of miR156 and miR172 regulates developmental timing in Arabidopsis. Cell 138: 750-759. doi:10.1016/j.cell.2009.06.031. PubMed: 19703400.
  46. 46. Zanca AS, Vicentini R, Ortiz-Morea FA, Del Bem LE, da Silva MJ et al. (2010) Identification and expression analysis of microRNAs and targets in the biofuel crop sugarcane. BMC Plant Biol 10: 260. doi:10.1186/1471-2229-10-260. PubMed: 21092324.
  47. 47. Arazi T, Talmor-Neiman M, Stav R, Riese M, Huijser P et al. (2005) Cloning and characterization of micro-RNAs from moss. Plant J 43: 837-848. doi:10.1111/j.1365-313X.2005.02499.x. PubMed: 16146523.
  48. 48. Carra A, Mica E, Gambino G, Pindo M, Moser C et al. (2009) Cloning and characterization of small non-coding RNAs from grape. Plant J 59: 750-763. doi:10.1111/j.1365-313X.2009.03906.x. PubMed: 19453456.
  49. 49. Li W, Cui X, Meng Z, Huang X, Xie Q et al. (2012) Transcriptional regulation of Arabidopsis MIR168a and argonaute1 homeostasis in abscisic acid and abiotic stress responses. Plant Physiol 158: 1279-1292. doi:10.1104/pp.111.188789. PubMed: 22247272.
  50. 50. Li W-X, Oono Y, Zhu J, He X-J, Wu J-M et al. (2008) The Arabidopsis NFYA5 transcription factor is regulated transcriptionally and posttranscriptionally to promote drought resistance. Plant Cell Available: 20: 2238-2251 PubMed: 18682547.
  51. 51. Chiou T-J, Aung K, Lin S-I, Wu C-C, Chiang S-F et al. (2006) Regulation of phosphate homeostasis by microRNA in Arabidopsis. Plant Cell Available: 18: 412-421 PubMed: 16387831.
  52. 52. Guo W, Wu G, Yan F, Lu Y, Zheng H et al. (2012) Identification of novel Oryza sativa miRNAs in deep sequencing-based small RNA libraries of rice infected with Rice stripe virus. PLOS ONE 7: e46443. doi:10.1371/journal.pone.0046443. PubMed: 23071571.
  53. 53. Gao Z, Shi T, Luo X, Zhang Z, Zhuang W et al. (2012) High-throughput sequencing of small RNAs and analysis of differentially expressed microRNAs associated with pistil development in Japanese apricot. BMC Genomics 13: 371. doi:10.1186/1471-2164-13-371. PubMed: 22863067.
  54. 54. Li H, Dong Y, Yin H, Wang N, Yang J et al. (2011) Characterization of the stress associated microRNAs in Glycine max by deep sequencing. BMC Plant Biol 11: 170. doi:10.1186/1471-2229-11-170. PubMed: 22112171.
  55. 55. Yin ZJ, Shen FF (2010) Identification and characterization of conserved microRNAs and their target genes in wheat (Triticum aestivum). Genet Mol Res 9: 1186-1196. doi:10.4238/vol9-2gmr805. PubMed: 20589616.
  56. 56. Mallory AC, Bartel DP, Bartel B (2005) MicroRNA-directed regulation of Arabidopsis AUXIN RESPONSE FACTOR17 is essential for proper development and modulates expression of early auxin response genes. Plant Cell 17: 1360-1375. doi:10.1105/tpc.105.031716. PubMed: 15829600.
  57. 57. Wu MF, Tian Q, Reed JW (2006) Arabidopsis microRNA167 controls patterns of ARF6 and ARF8 expression, and regulates both female and male reproduction. Development 133: 4211-4218. doi:10.1242/dev.02602. PubMed: 17021043.
  58. 58. Fu XY, Zhang Z, Peng RH, Xiong AS, Liu JG et al. (2007) Isolation and characterization of a novel cDNA encoding ERF/AP2-type transcription factor OsAP25 from Oryza sativa L. Biotechnol Lett 29: 1293-1299. doi:10.1007/s10529-007-9370-1. PubMed: 17516027.
  59. 59. Oh IH, Reddy EP (1999) The myb gene family in cell growth, differentiation and apoptosis. Oncogene 18: 3017-3033. doi:10.1038/sj.onc.1202839. PubMed: 10378697.
  60. 60. Toledo-Ortiz G, Huq E, Quail PH (2003) The Arabidopsis basic/helix-loop-helix transcription factor family. Plant Cell 15: 1749-1770. doi:10.1105/tpc.013839. PubMed: 12897250.
  61. 61. Dhondt S, Coppens F, De Winter F, Swarup K, Merks RM et al. (2010) SHORT-ROOT and SCARECROW regulate leaf growth in Arabidopsis by stimulating S-phase progression of the cell cycle. Plant Physiol 154: 1183-1195. doi:10.1104/pp.110.158857. PubMed: 20739610.
  62. 62. Udvardi MK, Czechowski T, Scheible WR (2008) Eleven golden rules of quantitative RT-PCR. Plant Cell 20: 1736-1737. doi:10.1105/tpc.108.061143. PubMed: 18664613.
  63. 63. Yakovlev IA, Fossdal CG, Johnsen O (2010) MicroRNAs, the epigenetic memory and climatic adaptation in Norway spruce. New Phytol 187: 1154-1169. doi:10.1111/j.1469-8137.2010.03341.x. PubMed: 20561211.
  64. 64. Lu S, Sun YH, Amerson H, Chiang VL (2007) MicroRNAs in loblolly pine (Pinus taeda L.) and their association with fusiform rust gall development. Plant J 51: 1077-1098. doi:10.1111/j.1365-313X.2007.03208.x. PubMed: 17635765.
  65. 65. Fattash I, Voss B, Reski R, Hess WR, Frank W (2007) Evidence for the rapid expansion of microRNA-mediated regulation in early land plant evolution. BMC Plant Biol 7: 13. doi:10.1186/1471-2229-7-13. PubMed: 17359535.
  66. 66. Axtell MJ, Snyder JA, Bartel DP (2007) Common functions for diverse small RNAs of land plants. Plant Cell 19: 1750-1769. doi:10.1105/tpc.107.051706. PubMed: 17601824.
  67. 67. Backman TW, Sullivan CM, Cumbie JS, Miller ZA, Chapman EJ et al. (2008) Update of ASRP: the Arabidopsis Small RNA Project database. Nucleic Acids Res 36: D982-D985. PubMed: 17999994.
  68. 68. Xie Z, Allen E, Fahlgren N, Calamar A, Givan SA et al. (2005) Expression of Arabidopsis MIRNA genes. Plant Physiol 138: 2145-2154. doi:10.1104/pp.105.062943. PubMed: 16040653.
  69. 69. Lu C, Kulkarni K, Souret FF, MuthuValliappan R, Tej SS et al. (2006) MicroRNAs and other small RNAs enriched in the Arabidopsis RNA-dependent RNA polymerase-2 mutant. Genome Res 16: 1276-1288. doi:10.1101/gr.5530106. PubMed: 16954541.
  70. 70. Sunkar R, Zhu JK (2004) Novel and stress-regulated microRNAs and other small RNAs from Arabidopsis. Plant Cell 16: 2001-2019. doi:10.1105/tpc.104.022830. PubMed: 15258262.
  71. 71. Fahlgren N, Howell MD, Kasschau KD, Chapman EJ, Sullivan CM et al. (2007) High-throughput sequencing of Arabidopsis microRNAs: evidence for frequent birth and death of MIRNA genes. PLOS ONE 2: e219. doi:10.1371/journal.pone.0000219. PubMed: 17299599.
  72. 72. Xie FL, Huang SQ, Guo K, Xiang AL, Zhu YY et al. (2007) Computational identification of novel microRNAs and targets in Brassica napus. FEBS Lett 581: 1464-1474. doi:10.1016/j.febslet.2007.02.074. PubMed: 17367786.
  73. 73. Wang L, Wang MB, Tu JX, Helliwell CA, Waterhouse PM et al. (2007) Cloning and characterization of microRNAs from Brassica napus. FEBS Lett 581: 3848-3856. doi:10.1016/j.febslet.2007.07.010. PubMed: 17659282.
  74. 74. Zhao YT, Wang M, Fu SX, Yang WC, Qi CK et al. (2012) Small RNA profiling in two Brassica napus cultivars identifies microRNAs with oil production- and development-correlated expression and new small RNA classes. Plant Physiol 158: 813-823. doi:10.1104/pp.111.187666. PubMed: 22138974.
  75. 75. Song C, Fang J, Li X, Liu H, Thomas Chao C (2009) Identification and characterization of 27 conserved microRNAs in citrus. Planta 230: 671-685. doi:10.1007/s00425-009-0971-x. PubMed: 19585144.
  76. 76. Zeng C, Wang W, Zheng Y, Chen X, Bo W et al. (2010) Conservation and divergence of microRNAs and their functions in Euphorbiaceous plants. Nucleic Acids Res 38: 981-995. doi:10.1093/nar/gkp1035. PubMed: 19942686.
  77. 77. Zhou ZS, Huang SQ, Yang ZM (2008) Bioinformatic identification and expression analysis of new microRNAs from Medicago truncatula. Biochem Biophys Res Commun 374: 538-542. doi:10.1016/j.bbrc.2008.07.083. PubMed: 18662674.
  78. 78. Jagadeeswaran G, Zheng Y, Li YF, Shukla LI, Matts J et al. (2009) Cloning and characterization of small RNAs from Medicago truncatula reveals four novel legume-specific microRNA families. New Phytol 184: 85-98. doi:10.1111/j.1469-8137.2009.02915.x. PubMed: 19555436.
  79. 79. Zhang L, Chia JM, Kumari S, Stein JC, Liu Z et al. (2009) A genome-wide characterization of microRNA genes in maize. PLOS Genet 5: e1000716.
  80. 80. Wei LQ, Yan LF, Wang T (2011) Deep sequencing on genome-wide scale reveals the unique composition and expression patterns of microRNAs in developing pollen of Oryza sativa. Genome Biol 12: R53. doi:10.1186/gb-2011-12-6-r53. PubMed: 21679406.
  81. 81. Jeong DH, Park S, Zhai J, Gurazada SG, De Paoli E et al. (2011) Massive analysis of rice small RNAs: mechanistic implications of regulated microRNAs and variants for differential target RNA cleavage. Plant Cell 23: 4185-4207. doi:10.1105/tpc.111.089045. PubMed: 22158467.
  82. 82. Jaillon O, Aury JM, Noel B, Policriti A, Clepet C et al. (2007) The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla. Nature 449: 463-467. doi:10.1038/nature06148. PubMed: 17721507.
  83. 83. Mica E, Piccolo V, Delledonne M, Ferrarini A, Pezzotti M et al. (2010) Correction: High throughput approaches reveal splicing of primary microRNA transcripts and tissue specific expression of mature microRNAs in Vitis vinifera. BMC Genomics 11: 109. doi:10.1186/1471-2164-11-109. PubMed: 20152027.
  84. 84. Wu L, Zhang Q, Zhou H, Ni F, Wu X et al. (2009) Rice MicroRNA effector complexes and targets. Plant Cell 21: 3421-3435. doi:10.1105/tpc.109.070938. PubMed: 19903869.