The proton-coupled folate transporter (PCFT) was recently identified as the major uptake route for dietary folates in humans. The three-dimensional structure of PCFT and its detailed interplay with function remain to be determined. We screened the water-accessible extracellular surface of HsPCFT using the substituted-cysteine accessibility method, to investigate the boundaries between the water-accessible surface and inaccessible buried protein segments. Single-cysteines, engineered individually at 40 positions in a functional cysteine-less HsPCFT background construct, were probed for plasma-membrane expression in Xenopus oocytes with a bilayer-impermeant primary-amine-reactive biotinylating agent (sulfosuccinimidyl 6-(biotinamido) hexanoate), and additionally for water-accessibility of the respective engineered cysteine with the sulfhydryl-selective biotinylating agent 2-((biotinoyl)amino)ethyl methanethiosulfonate. The ratio between Cys-selective over amine-selective labeling was further used to evaluate three-dimensional models of HsPCFT generated by homology / threading modeling. The closest homologues of HsPCFT with a known experimentally-determined three-dimensional structure are all members of one of the largest membrane protein super-families, the major facilitator superfamily (MFS). The low sequence identity - 14% or less – between HsPCFT and these templates necessitates experiment-based evaluation and model refinement of homology / threading models. With the present set of single-cysteine accessibilities, the models based on GlpT and PepTSt are most promising for further refinement.
Citation: Duddempudi PK, Goyal R, Date SS, Jansen M (2013) Delineating the Extracellular Water-Accessible Surface of the Proton-Coupled Folate Transporter. PLoS ONE 8(10): e78301. doi:10.1371/journal.pone.0078301
Editor: Hendrik W. van Veen, University of Cambridge, United Kingdom
Received: June 26, 2013; Accepted: September 16, 2013; Published: October 18, 2013
Copyright: © 2013 Duddempudi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported, in part, by grants provided by the Center for Membrane Protein Research, TTUHSC (Texas Tech University Health Sciences Center)(to MJ), and by the seed-grant program of the Office of Research, TTUHSC (to MJ). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Folate (vitamin B9) belongs to the water-soluble B vitamin group. The name is derived from the Latin word for leaf (folium), as green leafy vegetables are naturally rich in folate. Oxidized and polyglutamate forms of folate are dietary active, but must be deconjugated to remove glutamic acid moieties and reduced to dihydrofolate and tetrahydrofolate to confer biological activity. These reduced compounds play important roles in one-carbon metabolism pathways such as biosynthesis of nucleic-acids and also in synthesis and breakdown of amino acids. Common to all vitamins, folates cannot be synthesized by mammals, and are therefore considered vital nutrients. Folates are highly absorbed from dietary sources in the intestine by specialized membrane proteins. Folate deficiency in pregnant women increases the risk for the development of neural tube defects (NTDs) in the offspring . Deficiency in adults may result in various pathological conditions including cardiovascular diseases and cancer [2,3]. However, adverse effects of high folate status include, decreased natural killer cell cytotoxicity, as well as progression and growth of preneoplastic cells . Due to its hydrophilic anionic structure, folic acid cannot freely diffuse across lipid bilayers, and efficient uptake into cells must therefore be facilitated by specialized membrane proteins. Specific transmembrane proteins that mediate folate uptake into cells are the proton-coupled folate transporter (PCFT), the reduced-folate carrier (RFC), and folate receptors (FRα and FRβ)[5-7]. These membrane proteins transport folate by different mechanisms and with different affinities and substrate specificities. PCFT and RFC are secondary-active transporters with optimum transport activity at acidic and neutral pH, respectively, while FRs are glycosylphosphatidylinositol (GPI) linked proteins and transport folates via endocytosis [8,9].
PCFT is highly expressed in the brush-border membrane of the proximal part of the small intestine, where folates are maximally absorbed . PCFT also transports folates from the blood into the cerebrospinal fluid through its expression on the basolateral membrane of ependymal cells in the choroid plexus [6,10]. Loss-of-function mutations of PCFT in humans disrupt these physiological roles and result in the development of a rare recessive autosomal disorder known as Hereditary Folate Malabsoption (HFM). Several mutations in the HsPCFT gene that result in HFM have been identified and characterized. Implications of HFM are low levels of folate in the blood and CNS, which result in megaloblastic anemia, immune deficiency, and neurological manifestations, including seizures, developmental delays, cognitive and motor impairment. PCFT expression was identified in various solid-tumor cell lines, where its acidic pH optimum, together with the acidic microenvironment surrounding these cells, facilitates transport of folates into solid tumors . Novel antifolates are synthesized that can be transported effectively through PCFT into human solid tumors .
PCFT belongs to the major facilitator superfamily (MFS) that comprises over 50 families. X-ray structures for prokaryotic members of six MFS families that utilize proton-cotransport and for one exchanger are currently available, but structural information about PCFT is very limited at present. PCFT is functional as a monomer in plasma membranes . Intracellular higher order oligomeric states have been observed under conditions of transient overexpression . Hydrophobicity analysis of HsPCFT’s amino acid sequence indicated that PCFT is composed of 12 transmembrane a-helices (TMI to TMXII) which was confirmed using a single-Cys in each loop between transmembrane segments, using the substituted-cysteine accessibility method (SCAM) [14,15]. PCFT contains two consensuses N-glycosylation sites (N58 and N68), located in the first extracellular loop between transmembrane segments 1 and 2 . Immunohistochemical analysis investigating the accessibility of an engineered HA epitope attached to PCFT showed that both N- and C-termini are located on the cytoplasmic side . Site-directed mutagenesis of residues identified in loss-of-function mutations of the HsPCFT gene involved in HFM have identified several functionally or/and structurally important elements in HsPCFT [18-27]. Server-based methods for homology-prediction and homology model-generation based on transporters with experimentally-determined - for example X-ray – three-dimensional structure can be applied to PCFT. However, due to the low sequence identity (14% or less) with identified templates, any model will require careful experimental validation. Previously, three-dimensional PCFT models were constructed using X-ray-derived structures of transporters with predicted structural homology, the glycerol-3-phosphate transporter (GlpT, 1pw4) or a multidrug transporter (EmrD, 2gfp) as templates [24,28]. Importantly, at present, six out of nine server-identified templates have been structurally characterized after the previous homology models were generated.
Accordingly, we designed the present study to generate and validate PCFT homology models. We utilized cysteine-scanning in combination with the substituted-cysteine accessibility method (SCAM) to investigate the extent of accessibility of all twelve transmembrane segments towards their respective extracellular ends and of the predicted extracellular loops towards the extracellular side of the plasma membranes. Initially, we manually generated a secondary structure model based on a three-dimensional homology model of PCFT built on the X-ray structure of GlpT as a template. We engineered 40 PCFT constructs, each containing a single-Cys in or close to an extracellular loop based on its location in our secondary structure model of PCFT. The plasma-membrane expression of each single-Cys construct was probed with sulfosuccinimidyl 6-(biotinamido) hexanoate (Sulfo-NHS-LC-biotin), and the accessibility of each engineered Cys was tested utilizing the sulfhydryl-selective reagent 2-((biotinoyl)amino)ethyl methanethiosulfonate (MTSEA-biotin). The Cys-accessibility normalized against the primary amine-accessibility was then used to evaluate nine homology models generated by HHpred/MODELLER and LOMETS/MODELLER. We identified the models based on GlpT and PepTSt most suitable for further studies.
Materials and Methods
Sulfosuccinimidyl 6-(biotinamido) hexanoate (sulfo-NHS-LC-biotin) and High Capacity NeutrAvidin Agarose Resin were purchased from Thermo Scientific (Rockford, IL, USA). MTSEA-biotin was obtained from Toronto Research Chemicals (Toronto, Ontario, Canada).
Site-directed mutagenesis of PCFT
A ctV5-HsPCFT-CYS- construct in the pXOON vector  was constructed by replacing all seven endogenous Cys residues in ctV5-HsPCFT pXOON (PCFT wild-type with a C-terminal V5-epitope tag, GKPIPNPLLGLDST) with serine, using the QuikChangeTM Lightning Site-Directed Mutagenesis kit (Agilent, Santa Clara, CA, USA). This construct was used as a template to generate 40 single-Cys mutants by site-directed mutagenesis using the QuikChangeTM kit (Agilent). We tried to engineer the Cys at positions such that the mutations were conserved. The mutagenesis primers were generated based on the manufacturer’s recommendations and sequences are available upon request. A total of 40 residues were mutated to Cys, one at a time, in all six predicted extracellular loops, and in the predicted extracellular ends of the transmembrane segments. All mutations were confirmed by DNA sequencing of the entire gene (Genewiz, South Plainfield, NJ, USA).
Xenopus laevis oocytes
Large, adult lab-bred female Xenopus laevis were purchased from Xenopus Express (Hamosassa, FL, USA). Oocytes were harvested from tricaine-anesthetized frogs, and washed in oocyte Ringer’s buffer, OR2 (in mM: 82.5 NaCl, 2 KCl, 1 MgCl2, and 5 HEPES; pH adjusted to 7.5 with NaOH) before treatment with collagenase (2 mg/ml type 1A, Sigma, St. Louis, MO, USA) in the same buffer for 45-75 minutes at room temperature. Oocytes were treated thoroughly with OR2 containing Ca for three 45-minute intervals with media changes in between each incubation. Oocytes were then maintained in standard oocyte saline (SOS) medium (in mM: 100 NaCl, 2 KCl, 1.8 CaCl2, 1 MgCl2, and 5 mM HEPES, pH 7.5), supplemented with 1% antibiotic–antimycotic (100x) liquid (10,000 IU/ml penicillin, 10,000 µg/ml streptomycin, and 25 µg/ml amphotericin B; Invitrogen, Carlsbad, CA, USA) and 5% horse serum (Sigma). Oocytes were injected with 50 ng of in-vitro synthesized mRNA 12 to 24 h after harvest, and subsequently incubated in horse serum media for 4-10 days at 16-18°C.
Radiosubstrate Uptake by PCFT-expressing X. laevis oocytes
PCFT mediated uptake of [3H]folic acid (Moravek Biochemicals, Inc., Brea, CA) into X. laevis oocytes was determined in MES buffered saline (MBS) buffer (140 mM NaCl, 2.8 mM KCl, 2 mM MgCl2, 1 mM CaCl2, 10 mM MES, pH 5.5). Transport of folic acid through PCFT is proton-coupled and therefore facilitated by acidic pH. Therefore, uptake was studied at pH 5.5 . Oocytes were washed 3-4 times with Hepes buffered saline (HBS) buffer (140 mM NaCl, 2.8 mM KCl, 2 mM MgCl2, 1 mM CaCl2, 10 mM HEPES, pH 7.4). Uptake was initiated by placing 3-5 oocytes into MBS buffer (pH 5.5) containing a 0.015 μM concentration of [3H]folic acid. After incubation for 10 min at room temperature, uptake was halted by 5-6 rapid washes with cold MBS buffer (pH 5.5). Oocytes were individually solubilized in 300 μl of 5% SDS for 60 minutes to overnight, and uptake of radiolabeled substrate was determined with a Packard 1900 TR liquid scintillation analyzer or a Beckman LS 6500 Scintillation Counter. To evaluate non-PCFT mediated folic acid uptake in oocytes, control experiments were performed with uninjected oocytes. Comparison of uptake in noninjected vs. water-injected oocytes showed no significant differences (data not shown). Uptake expressed in picomoles of [3H]folic acid per oocyte.
Biotinylation of Xenopus laevis oocytes with Sulfo-NHS-LC-biotin
4-5 days after injection, oocytes were washed three times with 6 ml of calcium-free OR-2. Surface proteins were biotinylated with 0.5 mg/ml sulfo-NHS-LC-biotin for 30 minutes at room temperature. Then the oocytes were washed three times with 6 ml of calcium-free OR-2 solution. The excess amount of sulfo-NHS-LC-biotin was quenched by incubating the oocytes for 10 minutes in buffer H (100 mM NaCl, 20 mM Tris, pH 7.4). The oocytes were triturated at 4°C in 20 μl/oocyte buffer H++ (buffer H with 1% Triton X-100, 0.5% deoxycholate, and 1x HALT protease inhibitor cocktail, Thermo Scientific), solubilized by rotating at 4°C for 60 minutes and spun at 21,000 g for 10 minutes at 4°C. After carefully removing the debris and yolk, the supernatant was again spun at 21,000 g for 10 minutes at 4°C to remove any residual debris and yolk. To isolate biotinylated proteins, the supernatant was incubated with prewashed and buffer H++-equilibrated neutravidin beads for 2 hours at 4°C. After incubation the beads were spun at 2,500 g for 2.5 minutes at room temperature to remove unbound proteins. The beads were washed three times with 1ml of buffer H++, with the last wash supplemented with 2% SDS. The biotinlyated proteins were eluted from the beads by adding 60 µl of 4X SDS-sample buffer with DTT. Samples were loaded on 4-15 % Precast criterion gels (Bio-rad), transferred to PVDF membranes, and probed with V5 HRP antibody (1:5,000 in 5% milk for 4 hours at room temperature).
Biotinylation of Xenopus laevis oocytes with MTSEA-biotin
The procedure followed for Cys-biotinylation was comparable to the one described for primary amine-biotinylation described above, except that MTSEA-biotin was used. Excess MTSEA-biotin was removed by washing extensively with OR-2.
Western blot analysis
Densitometry analysis of the Western blots was performed with the AlphaEaseFC software after image capture with the Fluor ChemSP Imager (Alpha Innotech Co., San Leandro, CA). For each mutant we determined the ratios between Western blot signals with ImageJ after labeling with the Cys-selective label (MTSEA-biotin) over the Lys-selective label (sulfo-NHS-LC-biotin) to quantify Cys-accessibility over surface expression. The accessibility ratios were binned into three groups with regard to the accessibility of the respective Cys: Cys not accessible (ratio ≤ 0.3), Cys medium accessible (0.3 < ratio < 1.0), Cys highly accessible (ratio ≥ 1.0).
Data shown are means ± SEM. One-way ANOVA with Bonferroni’s multiple comparison test or unpaired t-test (GraphPad Prism v5.0 software) was used to calculate statistical difference in [3H]folic acid uptake between HsPCFT wild-type and HsPCFT-CYS- injected oocytes.
Homology Modeling of PCFT
The HHpred server together with MODELLER (http://toolkit.tuebingen.mpg.de/hhpred) was used to search for remote homologues of PCFT (query date: Apr 26, 2013, GenBank accession number NP_NM_080669.4) with experimentally-determined crystal structures in the PDB database and to generate structural models using default parameters . Similarly, LOMETS/MODELLER (http://zhanglab.ccmb.med.umich.edu/LOMETS/) was used to generate additional models.
Homology Modeling of PCFT
HHpred identified nine hits with ≥ 99.9 % probability [31,32]. Six hits are E. coli proteins, glycerol-3-phosphate transporter (GlpT, PDB # 1pw4), multidrug resistance protein (EmrD, PDB # 2gfp), L-fucose-proton symporter (FucP, PDB # 3o7q), D-Xylose-proton symporter (XylE, PDB # 4gc0), lactose permease (LacY, PDB # 2cfq), nitrite extrusion protein (NarU, PDB # 4iu9). Two more hits are of prokaryotic origin, tripeptide-proton symporter (PepTSt, PDB # 4aps), and proton-peptide symporter (PepTSo, PDB # 2xut), and one hit of eukaryotic origin, phosphate transporter (PipT, PDB # 4j05). The E-values ranged between 7.5x10-24 to 1.8x10-38, and sequence identities between 9 and 14 % (Table 1). LOMETS identified the following templates with high confidence scores: GlpT, LacY, and EmrD. GlpT scored as the best template in HHpred and several sub-algorithms of LOMETS and therefore we chose it as the most suitable template to construct an initial homology model of PCFT. The alignment between PCFT and GlpT obtained from the HHpred server was used as input for structure modeling with the SWISS-MODEL server to generate a three-dimensional homology model of PCFT [42,43] that was subsequently used to generate a two-dimensional secondary structure of PCFT (Figure 1). For all identified templates default server settings were used to generate homology models with MODELLER.
|Gene||MFS member||E-value||% identity||PDB #@||Resolution [Å]||Release date MM/YYYY|
|GlpT||glycerol-3-phosphate transporter||3.7 x 10-37||14||1pw4%,&||3.30||08/2003|
|EmrD||multidrug resistance protein||1.8 x 10-38||13||2gfp%,&||3.50||05/2006|
|PipT||phosphate transporter||4.4 x 10-32||11||4j05%||2.90||04/2013|
|FucP||fucose-proton symporter||3.9 x 10-29||13||3o7q%||3.20||09/2010|
|XylE||D-Xylose-proton symporter||3.5 x 10-28||12||4gc0%||2.60||10/2012|
|LacY||lactose permease||7.4 x 10-31||12||2cfq%||2.95||03/2006|
|NarU||nitrite extrusion protein||3.1 x 10-29||9||4iu9%||3.01||04/2013|
|PepTSt||tripeptide-proton symporter||4.4 x 10-25||14||4aps%,&||3.30||06/2012|
|PepTSo||proton-peptide symporter||7.5 x 10-24||13||2xut%||3.62||12/2010|
(A) A three-dimensional homology model of PCFT was built by the SWISS-MODEL server using an HHpred generated alignment with GlpT as input and the crystal structure of GlpT as a template. View parallel to the membrane showing all twelve transmembrane segments, color-coding as in panel C. (B) View perpendicular to the membrane looking into the predicted aqueous translocation pathway from the extracellular side. (C) Cartoon representation of the secondary structure of PCFT generated from the homology model by extrapolating information regarding the extent and orientation of transmembrane segments and loops. Gray-filled circles represent residues individually mutated to Cys. Black-filled circles identify endogenous Cys that were mutated to Ser. Numbering of extracellular loops (ECL) and intracellular loops (ICL) as indicated. Glycosylation sites indicated by “*”, and ECL surface Lys by “+”. Note, that the two Cys in ECL1 and ECL4 were also used as single-Cys mutants.
Cys-less PCFT is expressed in plasma membranes and functional
A Cys-less functional human PCFT (HsPCFT) construct with a C-terminal hemagglutinin-tag (HA) and with all seven endogenous Cys replaced by Ser has been previously expressed in HeLa cells using the vector pcDNA3.1 [15,16]. We have generated a HsPCFT construct with a C-terminal V5 epitope tag using the vector pXOON (ct-V5-HsPCFT). We substituted all seven endogenous Cys in our ct-V5-HsPCFT wild-type construct in the pXOON vector with Ser to obtain a Cys-less PCFT construct (ct-V5-HsPCFT-Cys-, short PCFT-Cys-) (Figure 1). We expressed ct-V5-HsPCFT with and without all endogenous Cys in Stage V X. laevis oocytes by micro-injecting the corresponding RNA. We first investigated if the Cys-less construct ct-V5-HsPCFT-Cys- was expressed in plasma membranes of X. laevis oocytes by surface-biotinylating plasma-membrane proteins with membrane-impermeant sulfo-NHS-LC biotin. The biotinylated proteins were isolated with avidin beads, separated by SDS PAGE, transferred to PVDF membranes and probed with an antibody against the C-terminal V5 epitope tag. We found ct-V5-HsPCFT and ct-V5-HsPCFT-Cys- to be robustly expressed at the plasma membrane. To ascertain that the construct retained its ability to translocate folic acid across the plasma membrane facilitated by a pH gradient, we performed uptake studies with [3H]folic acid at pH 5.5 (Figure 2). No statistical significant difference between [3H]folic acid influx mediated by HsPCFT wild-type and HsPCFT-Cys- injected oocytes was observed.
Five days after the oocytes were injected with WT PCFT and PCFT CYS- mRNA, the uptake of 0.015 µM [3H]folic acid was measured at pH 5.5 over 10 minutes. Transport of folic acid through PCFT is proton-coupled and therefore facilitated by acidic pH. Therefore, uptake was studied at pH 5.5 . Uninjected or water-injected oocytes (Uninj) were used as negative controls. Data represent the mean ± SEM from three different batches of oocytes. One-way ANOVA with Bonferroni’s multiple comparison test results shown. *** indicates p < 0.001; ns indicates not significant. Wild-type and Cys- are also not significantly different when analyzed with unpaired t test.
Extracellular accessibility of single-Cys PCFT constructs with sulfo-NHS-LC-biotin and MTSEA-biotin
To probe and optimize the obtained homology models of PCFT based on various X-ray-crystallography-derived templates by utilizing the substituted-cysteine accessibility method (SCAM), we used the functional Cys-less PCFT construct described above as template for generating 40 single-Cys mutants of PCFT (Figure 1). All constructs were confirmed by sequencing the entire coding region.
It has been observed in previous studies that the plasma-membrane expression levels of different single-Cys mutants can vary widely . We therefore used the plasma-membrane expression level to normalize our SCAM results. To evaluate the external accessibility of the single-Cys mytants we injected the corresponding RNA into X. laevis oocytes, and applied two procedures to each batch of oocytes in parallel: 1) we assessed the plasma-membrane expression level of each single-Cys construct individually, and 2) we probed the external accessibility of the engineered Cys. To investigate the plasma-membrane expression in X. laevis oocytes we used surface-biotinylation with sulfo-NHS-LC-biotin. Intact oocytes were treated with sulfo-NHS-LC-biotin. Due to the low pKa of the sulfo moiety this biotinylation compound is charged at our experimental labeling pH, and therefore it is water soluble and unable to cross biological membranes. The reactive NHS moiety selectively labels primary amine groups as found in Lys sidechains and the N-terminal primary amine group. If labeling is performed on intact cells, only Lys exposed to the surface can be modified. Since modification introduces a biotin, biotinylated surface proteins including labeled PCFT can be isolated after cell lysis with avidin beads. Avidin-bead-purified proteins were separated by SDS-PAGE and PCFT detected with an antibody against the V5-epitope tag engineered at its C-terminus. PCFT wild-type and Cys-less PCFT are efficiently surface-biotinylated by this reagent and detected in Western blots (Figure 3).
(A) Fifteen residues were selected within and close to ECL1 and ECL2. (B) Six residues were investigated in each ECL3 and ECL4. (C) Seven residues each were probed in ECL5 and ECL6. NH2-biotinylation shows Western blot after plasma membrane fraction isolation following surface-NH2 biotinylation with sulfo-NHS-LC-biotin / avidin beads. Uninjected oocytes (uninj.) served as negative control, and PCFT V5 and PCFT CYS- injected oocytes served as positive control. SH-biotinylation shows Western blot after avidin isolation of MTSEA-biotin labeled Cys. Single-Cys engineered at positions indicated on top of the lanes. Equal amounts of protein samples were electrophoretically separated on 4-15 % precast gels, transferred to PVDF membranes, and probed with V5-HRP antibody (1/5,000 dilution in 5 % milk). The experiment was repeated on three different batches of oocytes and a representative Western blot is shown for each condition. Lanes in boxes are from separate experiments as additional positions were probed later.
To probe the Cys accessibility a similar procedure was used after labeling with MTSEA-biotin. Under our reaction conditions MTSEA-biotin does not label PCFT wild-type or Cys-less PCFT (Figure 3). Additionally, in our hands MTSEA-biotin does not have access to internal Cys. Labeling of Cys in intracellular loops with MTSEA-biotin was only observed when oocytes were treated concomitantly with a permeabilizing agent like digitonin (10 µM, data not shown).
Subsequently, we engineered Cys in all six extracellular loop-helix boundary areas, such that at least one position approximately at the center of the loop, at least one position at the start of both the adjoining N- and C-terminal α-helical segments, and at least one position inside both the adjoining N- and C-terminal α-helical segments were mutated individually to Cys. Consequently, for each of the six ECL regions, we tested 4-8 individual positions, for a total of 40 engineered Cys.
The plasma-membrane expression levels, determined by sulfo-NHS-LC-biotin labeling of 38 single-Cys constructs, were sufficiently high to infer further the accessibility of the engineered Cys with MTSEA-biotin. The plasma-membrane expression for the single-Cys constructs varied substantially (Figure 3).
In the N-terminal domain of PCFT that comprises transmembrane segments I through VI, we tested a total of 21 positions. For the ECL1 region we probed nine positions that span a 45 residue segment, Q45, H49, S52, N58, C66, T74, T82, T86, and M89. For H49C the plasma-expression level was too low to make an assumption on the accessibility of the Cys. The other eight Cys constructs yielded good plasma-membrane expression (Figure 3). The ratio between Cys-labeling over Lys-labeling was quantified for all position and arbitrarily assigned to three bins, non-reactive, somewhat reactive, and highly reactive (Figure 4). Q45C and M89C were not accessible towards MTSEA-biotin. S52C, N58C, and T86C were somewhat accessible, and C66, T74C, and T82C were highly accessible (Figure 3, Figure 4). ECL2 region comprised six tested positions covering a 15 position amino acid stretch, V132, Q138, L139, V141, Y143, and L146. The plasma-membrane expression level of Q138C was too low to further investigate this position. Three positions, V132C, Y143C, and L146C were not accessible and two positions, L139C and V141C were highly accessible. Six positions as far apart as 18 positions were investigated in the ECL3 region, S196, L198, G207, N210, F212 and, L214. Here, one position, G207C, was highly accessible, four positions, S196C, L198C, N210C, and F212C, were somewhat accessible, and the L214C position was not accessible.
(A) ECL1 and ECL2. (B) ECL3 and ECL4. (C) ECL5 and ECL6. (D) Additional positions from all ECLs. Results shown in individual panels represent positions investigated on the same Western blot.
Out of the C-terminal domain that contains transmembrane segments VII through XII, we assayed a total of 19 positions in the remaining three ECLs. In ECL4 we engineered six single-CYS spanning a 16 amino acid segment at the following positions: A284, L288, E292, C298, and G307. Only one position was not accessible, A284C, while four were highly accessible, L288C, C298C, and G307C, and E292C was somewhat accessible. In ECL5 seven positions along a 19 amino acid long stretch were tested, V347, A350, T353, L357, F359, T360, and L365. Five were not accessible, V347C, A350C, T353C, T360C, and L365C. L357C was somewhat assessable whereas F359 was highly accessible. In the last ECL, ECL6, a segment covering an 18-amino acid segment with six engineered Cys, S407, N411, T417, M421, F424, and L427, was investigated. All positions here, except L427C that was not accessible, were highly accessible.
We utilized Cysteine scanning to investigate the loop-helix boundaries of the six extracellular loops in the 12-transmembrane segment MFS protein PCFT. In a previous study one residue from each extracellular loop was investigated to determine its external accessibility showing that C66, V141, G207, E292, L357 and T417 residues are externally accessible as these were labeled by MTSEA-biotin . The experimental results in this study further substantiate the previously established extracellular loops of PCFT. The present study additionally extends the experimentally-derived accessibility of the extracellular surface of PCFT by firstly studying 40 instead of only 6 positions, and secondly quantifying the extent of accessibility by applying a ratiometric approach, i.e., Cys-labeled over Lys-labeled. The positions were chosen such that the substitution of the endogenous residues by Cys was a conserved mutation.
The reactivity of individual Cys may be influenced by a variety of factors to include: access to the bulk solution and the pKa of the respective Cys. Access to the bulk solution is influenced by steric factors as well as the electrostatic environment along the pathway to the Cys. It is expected to be very high at the protein surface and in large water-filled cavities. The pKa of a Cys in a protein environment is crucial, since MTS reagents react 109 times faster with deprotonated thiolates over thiol Cys . Most Cys SH have a pKa of 8.5 to 9, however, closely-apposing positively-charged residues and appropriately oriented aromatic residues can lower it by five orders of magnitude, to as low as 3.5 [47,48]. Additionally, lipid-buried Cys are not expected to ionize to a significant extend and are much less likely to react.
The aim of our study was to investigate the extent of accessibility of residues in the extracellular ends of transmembrane helices and extracellular loops towards the extracellular milieu using the membrane impermeable Cys-modifying agent MTSEA-biotin. All Cys were engineered in a Cys-less background construct that had all seven native Cys replaced by Ser and that - when injected into oocytes - exhibited WT PCFT-like transport of [3H]folic acid at pH 5.5 (Figure 2). Many membrane proteins have been prepared as Cys-less versions with similar to wildtype functionality for Cys-scanning methods [14,49-53]. However, instances where a Cys-less construct was non-functional or the Cys-substituting amino acids had to be optimized have also been reported. For PCFT it has been shown previously that Cys-less PCFT translocates folic acid substrates similarly to wildtype [15,16]. Only two out of the seven Cys of PCFT are involved in disulfide bond formation . Since it has been shown that the plasma-membrane expression of Cys-less constructs can be decreased, we have normalized Cys-accessibility as determined with MTSEA-biotin in our present study against plasma-membrane expression levels .
A large extracellular loop was predicted between TM1 and TM2 (Figure 5) [45,55,56]. Indeed, we found that 5 residues, from a stretch of a total of 34 amino acids, are Cys-accessible. On both the N- and C-terminal ends we found one position that was not accessible. Likely, these two positions are part of the respective α-helical segments of TM1 and TM2 where access to the bulk solution is limited either by compact packing with neighboring amino acids or lipids. The loop between TM3 and TM4 is very short, and two close-by positions that are highly accessible (L139C and V141C) are framed by inaccessible residues in close proximity. S196C is accessible from the extracellular environment even though it is positioned at 1/3 of the distance from the start of transmembrane segment 5. Similarly, L198, that is almost one α-helical turn towards the extracellular environment, is also accessible. S196C may be facing the proposed hydrophilic substrate pathway, and L198C may be at the lipid-protein interface in the head-group region.
Importantly, we identified one unusual stretch of highly-accessible engineered Cys (S407-N411-T417-M421-F424). The first three are located on the extracellular end of TM11, and the latter two on the extracellular end of TM12. The location of S407C and N411C is below the bilayer level; however, these positions face the center of our PCFT model based on GlpT, and are likely lining the extracellular mouth of PCFT in the outward-facing state. A recent report investigated P425 extracellular accessibility with MTSEA-biotin and found that it is not accessible . The neighboring residue (F424), is labeled by MTSEA-biotin, proving its accessibility to the extracellular environment.
Overall, the extracellular water-accessibility of specific positions that are part of loop-helix regions on the extracellular surface of PCFT was determined. The loop between TM1 and TM2 is the largest extracellular loop, extending approximately from S42 to T82. Additionally, several residues that we predicted to be below the plasma membrane level based on our initial homology model were also labeled by MTSEA-biotin, likely because their side chains are accessible to potential hydrophilic substrate or proton-translocation pathway(s) at the center of PCFT.
Our ratiometric approach allows to rationally investigate homology models generated by server-based structure-prediction methods that identify distant homologues within the PDB database. If we simply color-code the three ratio-bins and apply this color code to the investigated 40 residues in the 9 homology models we generated with HHpred/MODELLER and LOMETS/MODELLER, it is immediately obvious that several models contradict our experimental observation, since the colors seem to be randomly distributed over the entire transmembrane depth of the modeled PCFT (Figure 6). For some models, however, the experimentally determined highly-accessible residues are at the extracellular protein surface, either because they are at local extracellular pikes of the protein, or directly lining a large cavity found in the extracellular-facing center of the protein, whereas somewhat accessible positions are partially buried, and non-accessible positions are in fact buried. The models based on the templates GlpT and PepTSt best reflect our current results.
Gene names are given above the respective panels, and pdb # and algorithm inside the top left corner of the upper panel for each model. View normal to the membrane in top row and from extracellular side in bottom row. The figures were prepared using the PyMOL Molecular Graphics System, Version 220.127.116.11 Schrödinger, LLC.
Further experiments are needed for rational improvement of the alignments used for generating the models, for example with MODalign. Even though the sequence identity between PCFT and the template transporters with presently known X-ray structures is 14% or less at present, the core region is expected to be structurally well conserved with an RMSD for backbone atoms within almost atomic resolution, making the predicted structure of this core region more reliable. By experimentally probing the more flexible ECL regions and in the future also ICL regions, and further experimentation to delineate the exact vertical alignment between individual TMs it should be possible to generate a homology model with a precision within the current X-ray structure determination range for membrane proteins. A verified model will be of great use for future studies investigating residues involved in substrate and proton translocation as well as mechanistic studies.
We thank Dr. Michael P. Blanton for generous assistance in uptake experiments and for helpful comments on the manuscript. We thank the TTUHSC Image Analysis Core Facility: some of the images and or data were generated in the Image Analysis Core Facility supported by TTUHSC.
Conceived and designed the experiments: PKD RG SD MJ. Performed the experiments: PKD RG SD. Analyzed the data: PKD RG SD MJ. Wrote the manuscript: PKD RG SD MJ.
- 1. Smithells RW, Sheppard S, Schorah CJ (1976) Vitamin dificiencies and neural tube defects. Arch Dis Child 51: 944-950. doi:10.1136/adc.51.12.944. PubMed: 1015847.
- 2. Blount BC, Mack MM, Wehr CM, MacGregor JT, Hiatt RA et al. (1997) Folate deficiency causes uracil misincorporation into human DNA and chromosome breakage: implications for cancer and neuronal damage. Proc Natl Acad Sci U S A 94: 3290-3295. doi:10.1073/pnas.94.7.3290. PubMed: 9096386.
- 3. Duthie SJ (1999) Folic acid deficiency and cancer: mechanisms of DNA instability. Br Med Bull 55: 578-592. doi:10.1258/0007142991902646. PubMed: 10746348.
- 4. Smith AD, Kim YI, Refsum H (2008) Is folic acid good for everyone? Am J Clin Nutr 87: 517-533. PubMed: 18326588.
- 5. Underhill TM, Flintoff WF (1989) Complementation of a methotrexate uptake defect in Chinese hamster ovary cells by DNA-mediated gene transfer. Mol Cell Biol 9: 1754-1758. PubMed: 2725523.
- 6. Qiu A, Jansen M, Sakaris A, Min SH, Chattopadhyay S et al. (2006) Identification of an intestinal folate transporter and the molecular basis for hereditary folate malabsorption. Cell 127: 917-928. doi:10.1016/j.cell.2006.09.041. PubMed: 17129779.
- 7. Antony AC, Utley C, Van Horne KC, Kolhouse JF (1981) Isolation and characterization of a folate receptor from human placenta. J Biol Chem 256: 9684-9692. PubMed: 6169714.
- 8. Henderson GB (1990) Folate-binding proteins. Annu Rev Nutr 10: 319-335. doi:10.1146/annurev.nu.10.070190.001535. PubMed: 2166548.
- 9. Antony AC (1992) The biological chemistry of folate receptors. Blood 79: 2807-2820. PubMed: 1586732.
- 10. Zhao R, Min SH, Wang Y, Campanella E, Low PS et al. (2009) A role for the proton-coupled folate transporter (PCFT-SLC46A1) in folate receptor-mediated endocytosis. J Biol Chem 284: 4267-4274. PubMed: 19074442.
- 11. Desmoulin SK, Wang Y, Wu J, Stout M, Hou Z et al. (2010) Targeting the proton-coupled folate transporter for selective delivery of 6-substituted pyrrolo[2,3-d]pyrimidine antifolate inhibitors of de novo purine biosynthesis in the chemotherapy of solid tumors. Mol Pharmacol 78: 577-587. doi:10.1124/mol.110.065896. PubMed: 20601456.
- 12. Duddempudi PK, Nakashe P, Blanton MP, Jansen M (2013) The monomeric state of the proton-coupled folate transporter represents the functional unit in the plasma membrane. FEBS J 280: 2900-2915. doi:10.1111/febs.12293. PubMed: 23601781.
- 13. Hou Z, Kugel Desmoulin S, Etnyre E, Olive M, Hsiung B et al. (2012) Identification and Functional Impact of Homo-oligomers of the Human Proton-coupled Folate Transporter. J Biol Chem 287: 4982-4995. doi:10.1074/jbc.M111.306860. PubMed: 22179615.
- 14. Akabas MH, Stauffer DA, Xu M, Karlin A (1992) Acetylcholine receptor channel structure probed in cysteine-substitution mutants. Science 258: 307-310. doi:10.1126/science.1384130. PubMed: 1384130.
- 15. Zhao R, Unal ES, Shin DS, Goldman ID (2010) Membrane topological analysis of the proton-coupled folate transporter (PCFT-SLC46A1) by the substituted cysteine accessibility method. Biochemistry 49: 2925-2931. doi:10.1021/bi9021439. PubMed: 20225891.
- 16. Unal ES, Zhao R, Qiu A, Goldman ID (2008) N-linked glycosylation and its impact on the electrophoretic mobility and function of the human proton-coupled folate transporter (HsPCFT). Biochim Biophys Acta 1778: 1407-1414. doi:10.1016/j.bbamem.2008.03.009. PubMed: 18405659.
- 17. Qiu A, Min SH, Jansen M, Malhotra U, Tsai E et al. (2007) Rodent intestinal folate transporters (SLC46A1): secondary structure, functional properties, and response to dietary folate restriction. Am J Physiol Cell Physiol 293: C1669-C1678. doi:10.1152/ajpcell.00202.2007. PubMed: 17898134.
- 18. Zhao R, Min SH, Qiu A, Sakaris A, Goldberg GL et al. (2007) The spectrum of mutations in the PCFT gene, coding for an intestinal folate transporter, that are the basis for hereditary folate malabsorption. Blood 110: 1147-1152. doi:10.1182/blood-2007-02-077099. PubMed: 17446347.
- 19. Shin DS, Mahadeo K, Min SH, Diop-Bove N, Clayton P et al. (2011) Identification of novel mutations in the proton-coupled folate transporter (PCFT-SLC46A1) associated with hereditary folate malabsorption. Mol Genet Metab 103: 33-37. doi:10.1016/j.ymgme.2011.01.008. PubMed: 21333572.
- 20. Shin DS, Min SH, Russell L, Zhao R, Fiser A et al. (2010) Functional roles of aspartate residues of the proton-coupled folate transporter (PCFT-SLC46A1); a D156Y mutation causing hereditary folate malabsorption. Blood 116: 5162-5169. doi:10.1182/blood-2010-06-291237. PubMed: 20805364.
- 21. Shin DS, Zhao R, Fiser A, Goldman DI (2012) Functional roles of the A335 and G338 residues of the proton-coupled folate transporter (PCFT-SLC46A1) mutated in hereditary folate malabsorption. Am J Physiol Cell Physiol 303: C834-C842. doi:10.1152/ajpcell.00171.2012. PubMed: 22843796.
- 22. Shin DS, Zhao R, Yap EH, Fiser A, Goldman ID (2012) A P425R mutation of the proton-coupled folate transporter causing hereditary folate malabsorption produces a highly selective alteration in folate binding. Am J Physiol Cell Physiol.
- 23. Lasry I, Berman B, Glaser F, Jansen G, Assaraf YG (2009) Hereditary folate malabsorption: a positively charged amino acid at position 113 of the proton-coupled folate transporter (PCFT/SLC46A1) is required for folic acid binding. Biochem Biophys Res Commun 386: 426-431. doi:10.1016/j.bbrc.2009.06.007. PubMed: 19508863.
- 24. Lasry I, Berman B, Straussberg R, Sofer Y, Bessler H et al. (2008) A novel loss-of-function mutation in the proton-coupled folate transporter from a patient with hereditary folate malabsorption reveals that Arg 113 is crucial for function. Blood 112: 2055-2061. doi:10.1182/blood-2008-04-150276. PubMed: 18559978.
- 25. Meyer E, Kurian MA, Pasha S, Trembath RC, Cole T et al. (2010) A novel PCFT gene mutation (p.Cys66LeufsX99) causing hereditary folate malabsorption. Mol Genet Metab 99: 325-328. doi:10.1016/j.ymgme.2009.11.004. PubMed: 20005757.
- 26. Min SH, Oh SY, Karp GI, Poncz M, Zhao R et al. (2008) The clinical course and genetic defect in the PCFT gene in a 27-year-old woman with hereditary folate malabsorption. J Pediatr 153: 435-437. doi:10.1016/j.jpeds.2008.04.009. PubMed: 18718264.
- 27. Atabay B, Turker M, Ozer EA, Mahadeo K, Diop-Bove N et al. (2010) Mutation of the proton-coupled folate transporter gene (PCFT-SLC46A1) in Turkish siblings with hereditary folate malabsorption. Pediatr Hematol Oncol 27: 614-619. doi:10.3109/08880018.2010.481705. PubMed: 20795774.
- 28. Unal ES, Zhao R, Chang MH, Fiser A, Romero MF et al. (2009) The functional roles of the His247 and His281 residues in folate and proton translocation mediated by the human proton-coupled folate transporter SLC46A1. J Biol Chem 284: 17846-17857. doi:10.1074/jbc.M109.008060. PubMed: 19389703.
- 29. Jespersen T, Grunnet M, Angelo K, Klaerke DA, Olesen SP (2002) Dual-function vector for protein expression in both mammalian cells and Xenopus laevis oocytes. BioTechniques 32: 536-538, 11911656.
- 30. Söding J (2005) Protein homology detection by HMM–HMM comparison. Bioinformatics 21: 951-960. doi:10.1093/bioinformatics/bti125. PubMed: 15531603.
- 31. Söding J (2005) Protein homology detection by HMM-HMM comparison. Bioinformatics 21: 951-960. doi:10.1093/bioinformatics/bti125. PubMed: 15531603.
- 32. Söding J, Biegert A, Lupas AN (2005) The HHpred interactive server for protein homology detection and structure prediction. Nucleic Acids Res 33: W244-W248. doi:10.1093/nar/gki162. PubMed: 15980461.
- 33. Huang Y, Lemieux MJ, Song J, Auer M, Wang DN (2003) Structure and mechanism of the glycerol-3-phosphate transporter from Escherichia coli. Science 301: 616-620. doi:10.1126/science.1087619. PubMed: 12893936.
- 34. Yin Y, He X, Szewczyk P, Nguyen T, Chang G (2006) Structure of the multidrug transporter EmrD from Escherichia coli. Science 312: 741-744. doi:10.1126/science.1125629. PubMed: 16675700.
- 35. Dang S, Sun L, Huang Y, Lu F, Liu Y et al. (2010) Structure of a fucose transporter in an outward-open conformation. Nature 467: 734-738. doi:10.1038/nature09406. PubMed: 20877283.
- 36. Sun L, Zeng X, Yan C, Sun X, Gong X et al. (2012) Crystal structure of a bacterial homologue of glucose transporters GLUT1-4. Nature 490: 361-366. doi:10.1038/nature11524. PubMed: 23075985.
- 37. Mirza O, Guan L, Verner G, Iwata S, Kaback HR (2006) Structural evidence for induced fit and a mechanism for sugar/H+ symport in LacY. EMBO J 25: 1177-1183. doi:10.1038/sj.emboj.7601028. PubMed: 16525509.
- 38. Yan H, Huang W, Yan C, Gong X, Jiang S et al. (2013) Structure and mechanism of a nitrate transporter. Cell Rep 3: 716-723. doi:10.1016/j.celrep.2013.03.007. PubMed: 23523348.
- 39. Solcan N, Kwok J, Fowler PW, Cameron AD, Drew D et al. (2012) Alternating access mechanism in the POT family of oligopeptide transporters. EMBO J 31: 3411-3421. doi:10.1038/emboj.2012.157. PubMed: 22659829.
- 40. Newstead S, Drew D, Cameron AD, Postis VL, Xia X et al. (2011) Crystal structure of a prokaryotic homologue of the mammalian oligopeptide-proton symporters, PepT1 and PepT2. EMBO J 30: 417-426. doi:10.1038/emboj.2010.309. PubMed: 21131908.
- 41. Pedersen BP, Kumar H, Waight AB, Risenmay AJ, Roe-Zurz Z et al. (2013) Crystal structure of a eukaryotic phosphate transporter. Nature 496: 533-536. doi:10.1038/nature12042. PubMed: 23542591.
- 42. Kiefer F, Arnold K, Künzli M, Bordoli L, Schwede T (2009) The SWISS-MODEL Repository and associated resources. Nucleic Acids Res 37: D387-D392. doi:10.1093/nar/gkn750. PubMed: 18931379.
- 43. Arnold K, Bordoli L, Kopp J, Schwede T (2006) The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling. Bioinformatics 22: 195-201. doi:10.1093/bioinformatics/bti770. PubMed: 16301204.
- 44. Mueckler M, Makepeace C (2005) Cysteine-scanning mutagenesis and substituted cysteine accessibility analysis of transmembrane segment 4 of the Glut1 glucose transporter. J Biol Chem 280: 39562-39568. doi:10.1074/jbc.M509050200. PubMed: 16172126.
- 45. Zhao R, Unal ES, Shin DS, Goldman ID (2010) Membrane Topological Analysis of the Proton-Coupled Folate Transporter (PCFT-SLC46A1) by the Substituted Cysteine Accessibility Method. Biochemistry 49: 2925-2931. doi:10.1021/bi9021439. PubMed: 20225891.
- 46. Roberts DD, Lewis SD, Ballou DP, Olson ST, Shafer JA (1986) Reactivity of small thiolate anions and Cysteine-25 in papain towards methylmethanethiosulfonate. Biochemistry 25: 5595-5601. doi:10.1021/bi00367a038. PubMed: 3778876.
- 47. Gan ZR, Sardana MK, Jacobs JW, Polokoff MA (1990) Yeast thioltransferase--the active site cysteines display differential reactivity. Arch Biochem Biophys 282: 110-115. doi:10.1016/0003-9861(90)90093-E. PubMed: 2221913.
- 48. Britto PJ, Knipling L, Wolff J (2002) The local electrostatic environment determines cysteine reactivity of tubulin. J Biol Chem 277: 29018-29027. doi:10.1074/jbc.M204263200. PubMed: 12023292.
- 49. Leng XH, Nishi T, Forgac M (1999) Transmembrane topography of the 100-kDa a subunit (Vph1p) of the yeast vacuolar proton-translocating ATPase. J Biol Chem 274: 14655-14661. doi:10.1074/jbc.274.21.14655. PubMed: 10329659.
- 50. Kasahara T, Kasahara M (2010) Identification of a key residue determining substrate affinity in the yeast glucose transporter Hxt7: a two-dimensional comprehensive study. J Biol Chem 285: 26263-26268. doi:10.1074/jbc.M110.149716. PubMed: 20525688.
- 51. Parikh RB, Bali M, Akabas MH (2011) Structure of the M2 transmembrane segment of GLIC, a prokaryotic Cys loop receptor homologue from Gloeobacter violaceus, probed by substituted cysteine accessibility. J Biol Chem 286: 14098-14109. doi:10.1074/jbc.M111.221895. PubMed: 21362624.
- 52. Holstead RG, Li MS, Linsdell P (2011) Functional differences in pore properties between wild-type and cysteine-less forms of the CFTR chloride channel. J Membr Biol 243: 15-23. doi:10.1007/s00232-011-9388-0. PubMed: 21796426.
- 53. Bao X, Chen Y, Reuss L, Altenberg GA (2004) Functional expression in Xenopus oocytes of gap-junctional hemichannels formed by a cysteine-less connexin 43. J Biol Chem 279: 9689-9692. PubMed: 14676187.
- 54. Zhao R, Shin DS, Goldman ID (2011) Vulnerability of the cysteine-less proton-coupled folate transporter (PCFT-SLC46A1) to mutational stress associated with the substituted cysteine accessibility method. Biochim Biophys Acta 1808: 1140-1145. doi:10.1016/j.bbamem.2011.01.008. PubMed: 21256110.
- 55. Zhao R, Sang HM, Qiu A, Sakaris A, Goldberg GL et al. (2007) The spectrum of mutations in the PCFT gene, coding for an intestinal folate transporter, that are the basis for hereditary folate malabsorption. Blood 110: 1147-1152. doi:10.1182/blood-2007-02-077099. PubMed: 17446347.
- 56. Qiu A, Sang HM, Jansen M, Malhotra U, Tsai E et al. (2007) Rodent intestinal folate transporters (SLC46A1): Secondary structure, functional properties, and response to dietary folate restriction. Am J Physiol Cell Physiol 293: C1669-C1678. doi:10.1152/ajpcell.00202.2007. PubMed: 17898134.
- 57. Shin DS, Zhao R, Yap EH, Fiser A, Goldman ID (2012) A P425R mutation of the proton-coupled folate transporter causing hereditary folate malabsorption produces a highly selective alteration in folate binding. Am J Physiol Cell Physiol 302: C1405-C1412. doi:10.1152/ajpcell.00435.2011. PubMed: 22345511.