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Household Water Chlorination Reduces Incidence of Diarrhea among Under-Five Children in Rural Ethiopia: A Cluster Randomized Controlled Trial

Household Water Chlorination Reduces Incidence of Diarrhea among Under-Five Children in Rural Ethiopia: A Cluster Randomized Controlled Trial

  • Bezatu Mengistie, 
  • Yemane Berhane, 
  • Alemayehu Worku
PLOS
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Abstract

Background

Household water treatment has been advocated as a means of decreasing the burden of diarrheal diseases among young children in areas where piped and treated water is not available. However, its effect size, the target population that benefit from the intervention, and its acceptability especially in rural population is yet to be determined. The objective of the study was to assess the effectiveness of household water chlorination in reducing incidence of diarrhea among children under-five years of age.

Method

A cluster randomized community trial was conducted in 36 rural neighborhoods of Eastern Ethiopia. Households with at least one child under-five years of age were included in the study. The study compared diarrhea incidence among children who received sodium hypochlorite (liquid bleach) for household water treatment and children who did not receive the water treatment. Generalized Estimation Equation model was used to compute adjusted incidence rate ratio and the corresponding 95% confidence interval.

Result

In this study, the incidence of diarrhea was 4.5 episodes/100 person week observations in the intervention arm compared to 10.4 episodes/100 person week observations in the control arm. A statistically significant reduction in incidence of diarrhea was observed in the intervention group compared to the control (Adjusted IRR = 0.42, 95% CI 0.36–0.48).

Conclusion

Expanding access to household water chlorination can help to substantially reduce child morbidity and achieve millennium development goal until reliable access to safe water is achieved.

Trial Registration

ClinicalTrials.gov NCT01376440

Introduction

Diarrheal disease kills 1.5 million people mostly children under the age of five years in developing countries each year [1]. Many of infectious agents causing diarrhea are potentially water borne transmitted through contaminated water [2]. Even though Millennium Development Goal (MDG) of drinking water is achieved, 780 million people lack access to improved water sources and 2.5 billion lack improved sanitation worldwide, rural population are disproportionately undeserved [3]. Even water from improved source is not always safe [4]. Furthermore, water collected from initially acceptable microbial quality, it often becomes contaminated with pathogens during transport and storage [5].

To overcome the difficulties in providing safe water, point-of-use water treatment has been advocated as a means to improve access to potable water and decrease the global burden of diarrheal diseases [2], [6], [7]. However, the effect of household water disinfection with chlorine on diarrhea episode reduction is variable, ranging from no protective effect to 85 percent reduction [8][16]. The studies on effectiveness of water quality interventions in reducing diarrhea have been flawed due to responder observer biases [2], [17]. Uptake and use is low among rural population who are more at risk of water borne disease [18]. It is difficult to identify the population that benefit most from the potential effect of the intervention [17].

Thus, the main aim of this intervention study was to determine the effectiveness of household water chlorination (point-of-use water treatment) in reducing diarrhea incidence among children under-five years of age in rural community of Eastern Ethiopia.

Methods

Ethics Statement

The study was reviewed and approved by the Haramaya University, College of Health Science Ethical Review Committee. Consent was obtained from district administration, district health department, community leaders. Written consent was also obtained from the primary caregivers of children. Field workers provided Oral Rehydration Solution (ORS) obtained from Kersa district health department for the children with diarrhea and advised their caregiver to take them to the nearby health facility for further treatment. Control communities received the intervention after the completion of the study. The protocol for this trial (Protocol S1) and supporting CONSORT checklist (Checklist S1) are available as supporting information.

Study Setting

The study was conducted in rural Kersa Demographic and Health Research Centre (KD-HRC) Field Site, Kersa district, Eastern Ethiopia. It is located in kersa district which is about 482 kms far from the capital, Adis Ababa. According to the 2007 baseline survey, the study area has a population of 47,036 (8960 households) distributed in 10 kebeles (smallest administrative unit with a population of 5,000) of which 7870 are children under-five years of age. All the households had no running water. Families collect water from springs, streams/rivers, or wells and store in 20 liter jerry-can.

Study Design and Procedure

We conducted randomized controlled, parallel group, field trial to assess the effectiveness of household chlorination in reducing diarrhea episode. The study site was selected by Haramaya University in 2007 to serve as demographic surveillance and health research center. It is one of the six Demographic Surveillance Sites (DSS) in the country. Ethiopian statistics authority has demarcated population enumeration villages (clusters) for 2007 Ethiopian population census across the country. Clusters are distinct neighborhoods with defined geographical boundaries. Rural KDS-HRC has 64 clusters and all were eligible for the study. Ethiopian central statistics authority statistician randomly selected 36 clusters from district population enumeration areas using computer generated random sampling. Field workers conducted census identified households in the selected clusters that had at least one child under-five years of age. Twenty four children (median of 16 households) were selected in each cluster by using simple random sampling from the list of households that contain the number of children in the presence of community leaders and some residents for follow up.

The randomization of clusters was done in a meeting with community leaders and representative from the health department. Each cluster code was written on a separate paper in front of the community leaders and put in a box. They agreed the first 18 draws to be assigned in the intervention arm and the remaining in the control. One of the community leaders draw 18 clusters consecutively from the box and assigned in the intervention arm. The remaining 18 clusters assigned in the control group. Field workers approached residents of selected households and completed baseline survey. Sodium hypochlorite (intervention) was distributed for all the households in the intervention arm. We used cluster level randomization to avoid ethical concerns and minimize the potential transfer of the intervention between the two groups (figure 1).

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Figure 1. Community randomized trial flow of participants on household water chlorination, eastern Ethiopia, 2011.

https://doi.org/10.1371/journal.pone.0077887.g001

Sample Size

The sample size was calculated using methods published by Hayes and Bennett [19], assuming 11% incidence of diarrhea among children in the control group based on previous study [20], 80% power, 10% drop out, 95% confidence interval and design effect of three from clustering. Accordingly, we aimed to enroll 18 clusters per arm with 24 children under the age of five years per cluster followed for 16 weeks (which would provide 6921 person weeks of observation in each group) to get sufficient power to detect 40% reduction in the incidence of diarrhea in the intervention group among children under-five years of age.

Intervention

The intervention for this study was 1.25% sodium hypochlorite branded locally known as “WaterGaurd” and obtained from population service international (PSI) manufactured specifically for home water disinfection. Local women were selected to distribute sodium hypochlorite for the intervention households with no charge to treat their stored water at home for 16-weeks from June to October 2011. They also explained how to treat water with chlorine and demonstrated how to use the disinfectant. Control groups continued their usual practice with respect to drinking water. Both intervention and control groups collect and store water with 20 liter jerry cans. In many African countries 20 liter jerry cans are used to transport and store water at home and are good options for safe storage [21].

Data Collection

A baseline survey was conducted on the demographic and socioeconomic condition, sources of water, access to and quality of water, water handling practice, sanitation, hygiene and pre-intervention diarrhea rates. The questionnaire was translated from English to the local language, Oromifa, back translated in to English and administered to the mother/caregiver by Local language.

The primary outcome was the occurrence of diarrhea among children under-five years of age. Diarrhea is defined as three or more loose or watery stools in 24 hours or more frequently than normal for an individual [1]. We defined a new episode of diarrhea if it occurred after a period of three diarrhea free days [22], [23]. We calculated the incidence of diarrhea as the number of new episodes divided by the total number of person–weeks observation [24]. The survey instrument was pre-tested in the nearby villages and amended based on the comments from the pretest. Field workers obtained data on the occurrence of diarrhea, water treatment practices and residual chlorine on weekly bases during the study.

The secondary outcome was compliance of the intervention. It was assessed on two unannounced and regular weekly visits using free residual chlorine measured with residual chlorine test kit (Wagetech 225 comparator color disc). It is a color wheel test kit that uses DPD (N,N diethyl-p-phenylene diamine) tablet.

Water samples were collected for bacteriological analysis from half of the randomly selected household water storage containers from both the intervention and the control villages at the baseline and the end of the study. Sterile 150 ml bottles were used for sample collection. The samples were transported to Haramaya University laboratory using ice packs and reached to the laboratory within 6 hours of collection. Multiple tube fermentation technique was used for determination of Escherichia coli which are regarded as the most reliable indicators of fecal contamination [6]. This technique is one of the standard methods in microbiological drinking water quality analysis [25].

Statistical Analysis

Data were double entered on to EPI data Version 3.1 and statistical analysis was performed using STATA Version 11. Mean was calculated to determine average compliance with the intervention. Intention- to- treat analysis was used to compare the incidence of diarrhea among children under-five years of age between intervention and control arms. We presented incidence data because is a better predictor of disease transmission, disease surveillance and control [24]. Generalized estimation equation (GEE) with log link Poisson distribution family was used to consider the repeated and clustered nature of the data. Crude and adjusted incidence rate ratio along with corresponding 95% confidence intervals was calculated to control the potential confounders. We used mixed effect logistic regression to obtain intera-cluster correlation coefficient (ρ).

Results

Participants and Baseline Characteristics

A total of 427 children in the intervention and 422 children in the control arm enrolled in 36 clusters. The number of children per cluster was 24. The median number of participating households with children under-five years of age per cluster was 16. Three households refused to give consent. Four households were lost to follow up. Data obtained from 425 children (284households) in the intervention group and 420 children (285 households) in the control group. The Follow up started in June 2011 and ended in October 2011. Data was collected on the occurrence of diarrhea for 13499 person week observation representing 98.03% and 97% of the total person week observation in intervention and controls groups respectively (figure 1). There was no any harm observed in this study.

The mean ages of the respondents and that of the children under the age of five years were 29.06 years and 26 months respectively. Only 8% mothers/caregivers had formal education. There was no significant difference in baseline demographic and socio-economic characteristics between the intervention and the control households.

Some of the households were getting water from well (49.2%), some from spring (40.6%) and the rest from stream (10.19%). Almost all households store their drinking water using jerry-cans. Most of the households did not treat their drinking water. There was no significant difference on source of water and water treatment practice between the two groups at the baseline. About 37.6% of the households had latrine. Before the intervention, the two week prevalence of diarrhea was 24.3% in the intervention households and 25.2% in the control households. Overall, at the baseline, the intervention and the control households had similar sanitation, hygiene and water handling practices. The two groups were similar in many potential confounding variables, as well (Table 1).

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Table 1. Baseline characteristics of community and household of the randomized control trial, Kersa district, Eastern Ethiopia, 2011.

https://doi.org/10.1371/journal.pone.0077887.t001

Diarrhea Incidence

From the control arm, 700 episodes of diarrhea (10.4 episodes per 100 person weeks observation) was reported but, from the intervention arm, it was 307 episodes (4.5 episodes per 100 person weeks observation). The effect of the intervention was different among the children with different age groups. It reduced 63% and 53% of the burden among children 3 to 4 years and 1 to 2 years of age respectively, while the reduction was lesser in less than one year children (44%) (Table 2). The relationship between study weeks and diarrhea in the intervention and control groups is shown in figure 2.

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Figure 2. Weekly prevalence of diarrhea versus weeks of observation, Kersa district, Eastern Ethiopia, 2011.

https://doi.org/10.1371/journal.pone.0077887.g002

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Table 2. Effect of the intervention with different age group of under-five children, Kersa district, Eastern Ethiopia, 2011.

https://doi.org/10.1371/journal.pone.0077887.t002

On the multivariable analysis adjusted for age, sex of the child, availability of latrine, waste disposal, availability of soap at home and hand washing facility, children in the intervention arm had lower risk of diarrhea (Adjusted RR = 0.42; 95% CI 0.37–0.49). There was a 58% overall reduction in the incidence of diarrhea among the intervention group compared to the controls (Table 3).

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Table 3. Multivariable analysis of intervention effect on the incidence of diarrhea among under-five children, Kersa district, Eastern Ethiopia, 2011.

https://doi.org/10.1371/journal.pone.0077887.t003

Use of the Intervention

Field workers measured the residual chlorine on a weekly basis throughout the study. During a weekly scheduled visit, an average 79.89% of the samples from the intervention households had free residual chlorine ≥0.2 mg/l (Figure 3). In unannounced visit 75.94% and 77.13% of the households had free residual chlorine in the 5th and 12th week respectively. Members of the households were not informed about the result of the free residual chlorine test. During the follow up 1.25% of the control households reported treating their drinking water.

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Figure 3. Percentage of residual chlorine during observation period, Kersa district, Eastern Ethiopia, 2011.

https://doi.org/10.1371/journal.pone.0077887.g003

Microbiological Quality

At the beginning and the end of the study drinking water samples were taken from the intervention and control household water storage containers. At the baseline, 78% of the households from the intervention (median E.coli MPN 70 per 100 ml) and 81% of those from the control households (median E.coli MPN 90 per 100 ml) were contaminated with E.coli. At the end of the study, however, 16.5% of the intervention households (median E.coli MPN 0 per 100 ml) and 65% control households (median E.coli MPN 60 per 100 ml) had E.coli in the sampled water. At the baseline, there was no significance difference between the two groups. However, drinking water samples from the intervention households were more likely to meet the WHO guidelines for bacteriological quality than samples from the control households at the end of the study (P<0.001) (Table 4).

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Table 4. Household stored water quality among intervention and control households at the baseline and end point of intervention.

https://doi.org/10.1371/journal.pone.0077887.t004

Discussion

A community based cluster randomized trial was conducted to assess the effectiveness of household water treatment with chlorine in reducing diarrhea among children under-five years of age. Household water treatment with sodium hypochlorite has reduced the incidence of diarrhea among children under-five years of age assigned to the intervention compared to the control (RR = 0.42 95% CI 0.37–0.49). There was significant improvement in the quality of stored water in intervention households.

Our result on reduction of diarrhea episode was consistent with similar studies conducted in Kenya [26] and Haiti [14]. But, the finding was higher than other studies from Bolivia [9] and Brazil [16]. The high magnitude of the protective effect in this study could be attributed to the high compliance of the intervention as observed during both scheduled and unannounced visits. Other study indicated that beneficial effect of such interventions may be greater in population where fecal contamination of drinking water is more likely [27]. We observed high fecal contamination of household stored water during the baseline thus that could be the reason for observing the desired effects of the intervention.

The household water treatment in this study was less effective among the younger children (less than one year) and the result is consistent with a study conducted in rural Guatemala [28]. This might be due to the high susceptibility and more chance of exposure to contaminated supplemental liquids among younger children.

High level of intervention compliance was achieved in the current study as observed in Zambia [10] and Gana [13]. Sub-group analysis within the intervention arm showed association between increased compliance and lower incidence of diarrhea (P = 0.014). Thus, the observed reduction in diarrheal episodes among the treatment group can be attributed to the intervention.

Household water chlorination is the most cost effective among all water quality interventions [29]. For instance, in the study area, 125 ml 1.25% sodium hypochlorite was bought $0.28 per bottle from retail shops or drug vendors, which can serve an average family for a month. This is about $ 0.00035 per liter that could be affordable for many of the households if they have access to the service. Using local community for the distribution of the disinfectant is a promising strategy to reach the rural community where the risk of diarrhea and other water borne diseases is high [30].

The primary outcome for this study was self reported diarrhea, which could be sensitive to recall bias. To minimize this recall bias, the data collection was on a weekly basis. This study was not blinded due to taste and odor of chlorine as well as ethical issue. Courtesy bias and Howthrone effect may overstate effectiveness of the HWT intervention in unblinded trials [2]. However, in this study, independent field workers were used for introducing the intervention and for collecting of data. Mausezahl et al recently indicated that in the absence of blinding using independent data collectors and intervention implementers is critical to reduce bias in HWT studies [31]. The investigators were not involved in the implementation of the intervention and data collection. The objective of the study was not stated for data collectors. We believe that sources of bias are addressed and their effects, if any, on the reported estimates are minimal.

In conclusion, home treatment of water with sodium hypochlorite significantly reduced the incidence of diarrhea among under-five children in the rural population where fecal contamination was high. We suggest that increasing access to such intervention would decrease child morbidity and mortality caused by diarrhea and help to achieve millennium development goal of 4 and 7.

Acknowledgments

The authors greatly acknowledge the households who participated in the study. We also thank our team of field workers for their careful data collection, Kersa district health department for providing Oral Rehydration Therapy, PSI Ethiopia for providing “WaterGuard” for the intervention.

Author Contributions

Conceived and designed the experiments: BM YB AW. Performed the experiments: BM YB AW. Analyzed the data: BM YB AW. Contributed reagents/materials/analysis tools: BM YB AW. Wrote the paper: BM YB AW.

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