The ideal conservation planning approach would enable decision-makers to use population viability analysis to assess the effects of management strategies and threats on all species at the landscape level. However, the lack of high-quality data derived from long-term studies, and uncertainty in model parameters and/or structure, often limit the use of population models to only a few species of conservation concern. We used spatially explicit metapopulation models in conjunction with multi-criteria decision analysis to assess how species-specific threats and management interventions would affect the persistence of African wild dog, black rhino, cheetah, elephant, leopard and lion, under six reserve scenarios, thereby providing the basis for deciding on a best course of conservation action in the South African province of KwaZulu-Natal, which forms the central component of the Maputaland-Pondoland-Albany biodiversity hotspot. Overall, the results suggest that current strategies of managing populations within individual, small, fenced reserves are unlikely to enhance metapopulation persistence should catastrophic events affect populations in the future. Creating larger and better-connected protected areas would ensure that threats can be better mitigated in the future for both African wild dog and leopard, which can disperse naturally, and black rhino, cheetah, elephant, and lion, which are constrained by electric fences but can be managed using translocation. The importance of both size and connectivity should inform endangered megafauna conservation and management, especially in the context of restoration efforts in increasingly human-dominated landscapes.
Citation: Di Minin E, Hunter LTB, Balme GA, Smith RJ, Goodman PS, Slotow R (2013) Creating Larger and Better Connected Protected Areas Enhances the Persistence of Big Game Species in the Maputaland-Pondoland-Albany Biodiversity Hotspot. PLoS ONE 8(8): e71788. doi:10.1371/journal.pone.0071788
Editor: Matt Hayward, Bangor University, United Kingdom
Received: August 27, 2012; Accepted: July 4, 2013; Published: August 14, 2013
Copyright: © 2013 Di Minin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The study was funded through a Panthera Kaplan Graduate Award and a US Fish & Wildlife Service Rhinoceros grant. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Conserving biodiversity with limited budgets requires allocating resources to actions that provide the highest return on investment . Prioritizing the allocation of limited resources to maximize conservation return requires accounting for the costs, benefits, and likelihood of success of alternative conservation actions . One of the best options to quantitatively measure the benefits of alternative conservation actions on the persistence of multiple species is to estimate the risk of extinction faced by each species using population viability analysis , . An important shortcoming of the use of population viability analyses is that they require extensive high quality data derived from long-term studies  and the effect of the conservation actions is generally a guess. Particularly, this is a limiting factor for many species of conservation concern for which little or no information is available . In addition, even with quality data, uncertainty in model parameters and/or structure is likely to affect the estimation of extinction probabilities .
The concept of population viability is pivotal to conservation planning and decision-making . Population models, for instance, can be used in an optimization framework, where a reserve selection algorithm is used to find a conservation solution that maximizes the viability of one or more species , . However, population models used in such frameworks tend to be simpler because of computing power limitations. Thus, population viability analyses have instead been used to provide information that can consequently be included in systematic conservation planning by: (1) producing data on the value of a given area to viability , ; (2) setting conservation targets for single or multiple species , ; and (3) determine cost-efficient protection strategies , . Alternatively, population viability analysis can be used to rank different conservation actions in a decision analysis context .
A decision analysis framework in combination with population viability analysis is considered one of the best ways to measure the effects of conservation actions on multiple species and account for uncertainty in population viability analysis , . A decision on the best course of conservation action is derived based on changes in the risk of extinction . Particularly, when constructing population models for multiple species, a decision model is required that considers the potentially diverging management needs of each species . Multi-criteria decision analysis, for instance, can be used to make a decision based on the ranking of alternative management strategies for each species . Alternatively, the probabilities of extinction can be combined to form a benefit or utility function . Otherwise, an index can be developed to combine assessments of viability for several species across the landscape .
The Maputaland-Pondoland-Albany biodiversity hotspot is internationally recognized for its high levels of species richness and endemism, which are under different levels of threat . Large mammal species were once widespread in the hotspot, but by the beginning of the 20th century they had declined dramatically or were driven to local extinction through over-hunting and persecution by humans . Recovery strategies, including one of the world's greatest conservation success stories, where the southern white rhinoceros (Ceratotherium simum simum) population increased from less than 20 individuals in the iMfolozi area in 1895 to more than 17,000 in the wild today , were then developed. More information is now required by decision-makers to inform the recovery strategies of great conservation value, such as the Black Rhino (Diceros bicornis minor) Range Expansion Project  and the African wild dog (Lycaon pictus) KwaZulu-Natal metapopulation management program , as well as enhance the conservation value of re-introductions of cheetah (Acynonix jubatus), elephant (Loxodonta africana) and lion (Panthera leo) , . Particularly, information is required on protected area size required to enhance the persistence of these species through management , . Although the metapopulation approach is being used as the cornerstone for the recovery of these species , , , populations are still heavily managed within individual, small, fenced reserves –.
In this study, we used multi-criteria decision analysis in combination with spatially explicit metapopulation models to create a rank order for six different reserve scenarios in order to decide which conservation action would best enhance the persistence of African wild dog, black rhinoceros, cheetah, elephant, leopard (Panthera pardus) and lion in the South African province of KwaZulu-Natal, which forms the central component of the Maputaland-Pondoland-Albany hotspot. The six study species are all listed under the IUCN Red List  and the Threatened or Protected Species List in South Africa . In addition, they are key flagship species for conservation in the region . Compared to previous studies , , this paper investigated the importance of protected area size, connectivity, and management, especially in the context of restoration efforts, in enhancing the persistence of wide-ranging species in human-dominated landscapes.
Study Area and Species
The KwaZulu-Natal province of South Africa has an extent of 92,000 km2 and forms the central component of the Maputaland-Pondoland-Albany biodiversity hotspot (Fig. 1) . This hotspot is the amalgamation of three centres of endemism (Maputaland, Pondoland and Albany) and encompasses six of South Africa‘s eight biomes . The topography ranges from ancient sand dunes and low-lying plains in the north to a series of rugged terraces deeply incised by river valleys in the central and southern parts. The climate ranges from subtropical/tropical in the low-lying northern coastal areas, to more temperate with frost in winter on the higher ground away from the coast .
The IUCN Red List threat status of the study species are as follows: black rhinoceros are classified as Critically Endangered; African wild dog as Endangered; lion, cheetah and elephant as Vulnerable; and leopard as Near Threatened . In South Africa, under the Threatened or Protected Species List, black rhino and African wild dog are classified as Endangered; cheetah, leopard and lion as Vulnerable; and elephant as Protected Species . In KwaZulu-Natal they are all classified as ‘specially protected game’ and fall under the mandate of the statutory conservation authority, Ezemvelo KwaZulu-Natal Wildlife, on both public and private lands (Fig. 1).
Population viability analysis was performed under six reserve scenarios, which were developed according to current constraints and future management opportunities in the study area. In the first scenario, named Small Reserve scenario hereafter, metapopulation dynamics were simulated for current conditions in which populations are constrained and managed within individual, fenced, protected areas , , . In the second scenario, named Big Reserve scenario hereafter, metapopulation dynamics were simulated for future conditions in which internal fences between all adjacent protected areas could be dropped in order to create larger protected areas. In the third scenario, named Small and Big Connected Reserve scenario hereafter, metapopulation dynamics were modelled for future conditions in which the most-connected big and small reserves from the Big and Small Reserve scenarios respectively could be selected. In the fourth scenario, named Big and Connected Reserve scenario hereafter, metapopulation dynamics were modelled for future conditions in which the big and small reserves from the Big and Small Connected Reserve scenario could be connected via designated linkages to create a network of large and connected protected areas. In the fifth scenario, named Bigger and Connected, but Cheap, Reserve scenario hereafter, metapopulation dynamics were modelled for future conditions in which the large reserves from the Big & Connected Reserve scenario could be expanded by including suitable habitat currently unprotected. Under the Bigger and Connected, but Cheap, Reserve scenario, we only included suitable habitat whereby conservation land-use was more profitable than alternative land uses , making it applicable to real-world protected area expansion. In the sixth and last scenario, named Biggest Reserve scenario hereafter, metapopulation dynamics were modelled for future conditions in which all suitable habitat could be protected. Under each reserve scenario external fences would be maintained, as best management practice, to separate biodiversity from threatening processes, and to constrain human-wildlife conflict . As a result, translocations were modelled for black rhino, cheetah, elephant, and lion to enhance gene flow among populations. For African wild dog and leopard, which cannot be constrained by fences , , dispersal was instead modelled based on distance between populations.
Spatially Explicit Metapopulation Models
A flow chart summarizing the modelling and decision analysis framework is provided in Figure 2. To analyse metapopulation viability of the six study species, we used the software RAMAS GIS 5.0 . Its wide application to different taxa and continued development makes RAMAS GIS a suitable tool for modelling spatial population dynamics of the study species . Furthermore, the software can include Allee effects, important for species such as the African wild dog , . First, the Spatial Data program was used to determine the metapopulation spatial structure under each reserve scenario mentioned above, using a habitat suitability map derived from species distribution models. Then an age-based matrix model was linked to each recognized population, allowing for spatial structure in population viability analysis and spatial variability in population dynamics. Under each reserve scenario, comprising a different number of populations for each species, several species-specific management and threat scenarios were then developed to understand how populations would react under previously not experienced conditions in the future. Based on the results of the metapopulation viability analysis, we then ranked the effectiveness of each reserve scenario in reducing the metapopulation probability of extinction using an outranking method . Conservation actions were ranked based on the probability of extinction because the objective of decision-makers in the area is to establish viable metapopulations of the study species through a shared commitment on private, community and public land –. All species are key flagships for conservation in the study area  and their re-introduction can potentially generate important funding. Hence, for the Bigger and Connected, but Cheap, Reserve scenarios, which looks at protected area expansion, we did a spatial cost-benefit analysis to evaluate the potential economic return from ecotourism, trophy hunting and live sales (conservation businesses) and then compared this return to 26 alternative land uses in the area so that we were able to select only areas where conservation land use is more profitable. Full details are provided in .
Species Distribution Models
We derived the habitat suitability map from species distribution models developed using MaxEnt . MaxEnt was selected among different presence-only modelling techniques available because it has been found to perform best . A presence-only modelling method was chosen as reliable absence data, and complete information on historical distributions of the species across the whole province, were missing. Occurrence, i.e. presence, data for the six study species were derived from historical records, on-going research and monitoring in the area, as well as surveys commissioned by the local conservation authorities. For on-going research and monitoring a combination of radio-telemetry and conventional tracking techniques were used to gather locations for all species , , , . The effect of sampling bias was examined by applying a spatial filter in an attempt to ensure a minimum geographical separation distance and increase the probability that observations were independent . Predictor variables were compiled to represent potential climatic and non-climatic (habitat and human impact) range controls. Variable selection was initially based on correlation tests to minimise potential collinearity issues , screening the number of variables used to a total of 23 (Table 1). The correlation test showed that only three slightly correlated pairs (median rainfall of the driest month vs the normalized difference vegetation index (NDVI) of the driest month; median rainfall of the wettest month vs NDVI of the wettest month; and elevation vs minimum temperature of the coldest month) were present (Pearson’s correlations, r <0.60), which could be expected . Despite these correlations, we decided to retain the correlated variables because they represented important, and mechanistically different, properties of the environment affecting species distributions. All statistical tests were performed using R v. 2.15.0 .
The non-climatic factors were represented by thirteen habitat and four human impact variables. The habitat variables representing the environmental variability of the area were aspect, digital elevation model (DEM), geology, slope and soil type. The slope and aspect grids were derived from DEM  in ArcEditor 9.2 . The geology and soil grids were derived from the Harmonised World Soil Database, which for Southern Africa is derived from regional SOTER (soil and terrain) studies . Additional habitat variables (distance from: bushland; dense bush; grassland; grassland/bushland; water; and woodland) were derived from the KwaZulu-Natal land cover map generated from multi-date spot 2/4 imagery , using the “Euclidean Distance” tool that gives the distance from each cell in the raster to the closest source . NDVI (for the driest and wettest months) was used as an estimate of vegetation production . NDVI values were calculated from 10 day composites of remotely sensed images from SPOT4 and SPOT5 satellites . As current anthropogenic factors are also believed to control species ranges , human impact was represented by three layers based on distance from different types of road, and one on human development, derived from the KwaZulu-Natal land cover map , using the “Euclidean Distance” tool in ArcEditor 9.2 . Predictor variables were re-sampled to WGS 1984 and cell size used was 0.005 decimal degrees in relation to the grid cell size of available environmental data and the characteristics of the occurrence data, which were geographically accurate and available at large numbers .
Species distribution models were then evaluated using the area under the curve (AUC) of the Receiver Operating Characteristic (ROC) curve. MaxEnt was run under the “auto-features” mode . The use of the default settings was reasonable, considering these were validated in studies with a wide range of species, environmental conditions, individual species records, and in cases with sample-selection bias . Default settings were also used in choosing at random 10000 background samples of pseudo-absences from the study area, and used them in place of absences during modelling to represent the environmental conditions in the region . The “replicates” option using cross-validation was used to do 10 runs for each species.
We selected the metapopulation structure for each species based on habitat suitability thresholds and the neighbourhood distance. For all species, the suitability threshold in MaxEnt was selected based on a balance between maximizing sensitivity and minimizing predicted area . Under the Biggest Reserve scenario, the metapopulation structure of each species was recognized by clustering together suitable cells if they were below a certain neighbourhood distance based on average home range size for each species , , , . Populations of black rhino, elephant, cheetah and lion were then split up if a national highway crossed them because electrified fences along them would prevent individuals from crossing them. Wildlife over-passes are currently not an option in the study area because they are too expensive to build. For leopard and African wild dog, which are not restricted by electrified fences , , instead, national highways were not considered as permanent barriers. Under the Small Reserve scenario, individual, fenced, protected areas were considered populations. Under the Big Reserve scenario, the spatial metapopulation structures were obtained in ArcEditor 9.2  by clipping out from each population recognized under the Biggest Reserve scenario suitable habitat currently protected, using a spatial layer where only adjacent protected areas were merged together. Under the Small and Big Connected Reserve scenario, we aggregated the most-connected big patches identified under the Big Reserve scenarios with the closest (but not directly adjacent) reserves identified under the Small Reserve scenarios. Under the Big and Connected Reserve scenario, the spatial metapopulation structures were obtained in ArcEditor 9.2  by merging the most-connected big and small patches identified under the Small and Big Connected Reserve scenarios via designated linkages. Under the Bigger and Connected, but Cheap, Reserve scenario, we added to the patches identified under the Big and Connected Reserve scenarios suitable habitat whereby conservation land use was more profitable than alternative land uses .
The permeability of the background matrix to dispersal between different populations was modelled for African wild dog and leopard only using a cost surface approach. Permeability was modelled for African wild dog and leopard only because, to prevent conflict with local communities and private landowners from occurring, all other species would continue being managed artificially within electrified fences under all Reserve scenarios. A friction surface, where each grid cell value represents the relative cost of dispersing through that cell, was used to recognize potential dispersal corridors from one population to another . Cost surface analysis allows incorporating matrix quality and dispersal barriers when evaluating the connectivity among populations. Matrix quality included areas with habitat suitability ≥0.5 outside identified populations, which were assigned the base cost of 1, or reflected the ability of African wild dog and leopard to move through various land cover types. In the latter case we simply estimated resistance to different land cover types, by applying information on habitat use of dispersing individuals for both species. The lowest cost (the areas of low resistance) of 1 was attributed to natural habitat (grassland, grassland and bushland, bushland, dense bush, woodland and forest). A cost of 10 was attributed to sugarcane farming and timber wood plantations and to provincial roads because both species were found dispersing through such land cover types before , . Settlements and urban areas were considered full dispersal barriers. As both African wild dog and leopard have been observed crossing national highways before and considering rivers dry up during dry season in the study area allowing wildlife crossing, a dispersal cost value of 200 was specified, implying dispersing through 200 cells of high-quality habitat (cost = 1 or HS ≥0.5) was equally costly than dispersing through one partial barrier.
To model metapopulation dynamics, we developed an age structured matrix model with annual time steps for each recognized population. Males and females were included as separate matrices for all species, but had the same age structures. Table 2 provides a summary of the most important model parameters included in the simulation. A detailed breakdown of the parameters and the matrices for each species is shown in Tables S1–S6 in Appendix S1. Initial population size and abundances were the same as actual populations, for which estimates were already available –.
Carrying capacity (K) for each modelled population was estimated by using different methods for carnivores and herbivores respectively. For elephant and black rhino, K was calculated by dividing the spatial extent of each population by its population density estimate. Specifically, we classified suitable habitat into low, medium and high suitability areas for both species. We then used a population density estimate based on forage/browse availability for each class (0.4, 0.6 and 0.8 elephant km−2 ; 0.1, 0.2 and 0.3 black rhino km−2 . For lion, leopard, cheetah and African wild dog, instead, we used a method, in which a predator would select prey from its preferred prey body mass range in proportion to the abundance of the same species in the same mass range to then calculate K . Specifically, the model that was used to calculate K for carnivores consisted of five different steps. The first step was to calculate prey biomass from the number of individuals available per prey species and mean mass of that species (Table S7 in Appendix S1) . The second step was to calculate prey biomass density by dividing prey biomass by the total suitable habitat area size in km−2. The third step was to estimate predator biomass density using prey biomass density and equations derived from . The fourth step was to calculate total predator biomass by dividing predator biomass density by the total suitable habitat area size in km−2. The fifth and final step was to calculate K by dividing total predator biomass by mean mass of predator , . Specific details about each step are provided in Appendix S1.
A dispersal-matrix, which defined dispersal rates based on distance between populations, was then developed for African wild dog and leopard only. Dispersal was defined as a function of K, whereby the dispersal rate was determined by the dispersal matrix when the population hit K. If the population was below K, then the dispersal rate decreased linearly as a function of K . For all other species, ‘artificial’ dispersal through translocation was modelled to ensure gene flow or restore populations, and implemented through the Population Management dialog box in RAMAS Metapop 5.0  (Tables S1–S6 in Appendix S1).
Demographic stochasticity was included in model simulations by sampling the number of survivors from a binomial distribution . Variability in environmental conditions can have strong influence on the survival and fecundity of the study species. Thus, environmental stochasticity was modelled by drawing values randomly from lognormal distributions described by the fecundity and survival values and their associated standard deviations . The effects of stochasticity on fecundity, survival, and K were assumed to be correlated within a population . The density-dependence function affecting all vital rates was modelled by modifying the mean values of survival rates and fecundities as a function of the population size  (see Tables S1–S6 in Appendix S1 for information on species-specific density dependence).
A sensitivity analysis was performed by creating a set of alternative models for each species, and comparing their results in RAMAS GIS. We did so to perform a parameter-by-parameter analysis of sensitivity . Specifically, this involved systematically changing the values of each input variable in absolute stepwise increments (±5 and 10%) while holding all other variables constant. The model parameters selected for sensitivity analysis were female and male survival in all age classes, fecundity, frequency and severity of catastrophe, initial population size, K, environmental stochasticity and maximum finite rate of increase.
Management and Threat Scenarios
We first considered a basic scenario under each reserve scenario in which metapopulation dynamics were modelled for existing management strategies and observed risk and severity of catastrophe (Table 3; Tables S1–S6 in Appendix S1). Species-specific threat and management scenarios were then performed under each reserve scenario in order to understand how metapopulations would respond to new environmental conditions, not previously experienced, in the future. Such scenarios were developed according to planned management strategies or potential threats faced by the study species in the area, making them applicable to real-world decision-making , – (Table 3). Each scenario was run over a short time interval of 50-year in order to minimize error propagation and evaluate conservative probabilities of extinction . At the end of each simulation, the metapopulation probability of extinction was recorded. As the simulation was repeated 1000 times, the metapopulation probability of extinction was estimated as the proportion of simulations that went extinct over the same timeframe.
Based on the metapopulation viability results, we used an outranking method  to compare the effectiveness of each model scenario in reducing the probability of extinction. The six species were treated as six separate criteria, and the PROMETHEE method was used to give an overall ranking of the reserve scenarios , . Practically, this required making pairwise comparisons of the basic, threat and management scenarios for the six alternative reserve scenarios for each species. The scenario with the lowest probability of extinction received 1 point and the scenario with the highest probability of extinction received no point. In case of a draw, no points were given at all. In order to account for simulation error within the decision-making framework, two scenarios were compared by using the Kolmogorov-Smirnov test statistic D of the terminal extinction risk curves with significance p<0.001 . By comparing all the scenarios, a preference matrix was constructed for each species. A total preference matrix was then constructed for all species using the preference matrices for each species. In the total preference matrix, the sum of each row gave the number of times that each scenario was preferred, and the sum of each column gave the number of times a scenario was beaten. Specifically, in the total preference matrix there were 18 criteria, which represented the six species times the three threat/management scenarios.
The species distribution models exhibited high average AUC values for the training and test dataset (0.95 and 0.91 respectively), indicating they were ‘highly accurate’ . Mean annual temperature and maximum temperature of the hottest month were the most important variables explaining the distributions of all species (Table S8 in Appendix S1). Soil types, elevation, geology, minimum temperature of the coldest month and median rainfall of the wettest month were also important variables affecting distributions. Distances from habitat variables were more important than NDVI in predicting distributions of all species. Distance from human development was the variable that decreased predictive performance the most for all species when removed from the simulation. The relative importance of all other variables was species-specific (Table S8 in Appendix S1).
Based on the habitat suitability maps (Figure 3), a different number of populations were identified under each reserve scenario for each species (Table 4). While the number of recognized populations decreased, mean, minimum and maximum carrying capacity increased from the Small to the Biggest Reserve scenarios (Table 4). The mean distance to other populations also decreased from the Small to the Biggest Reserve scenarios (Table 4). However, the Small & Big Reserve scenarios and the Bigger & Connected, but Cheap, Reserve scenarios were found to be better connected than the Big and the Biggest Reserve scenarios respectively (Table 4).
The results of the metapopulation dynamics for each species show that elephant, black rhino and leopard are at very low risk of extinction under basic Small Reserve scenarios (<5%), whilst African wild dog, cheetah and lion are at considerable risk of extinction (>25%) (Table 5). However, under the Small Reserve scenario the probability of extinction of elephant, black rhino and leopard increased as a consequence of increased risk and severity of poaching and disease (Table 5). Particularly, under the Small Reserve scenarios, African wild dog and cheetah will be under considerable risk of extinction if disease were to impact the populations more severely in the future, and mitigation strategies will not be able to offset negative effects.
Under the Big Reserve scenarios the probability of extinction decreased for all species, but remained high for cheetah under the basic, threat and threat mitigation scenarios (>22%), as well as black rhino, elephant and leopard under increased severity of poaching in the future (>29%). The probability of extinction decreased for all species under both the Small and Big Connected Reserve scenarios and the Big and Connected Reserve scenarios, but was higher for the former. While under the Small & Big Connected Reserve scenario African wild dog, cheetah and leopard remained at high risk of extinction, under the Big and Connected Reserve scenarios only the leopard metapopulation would still be at high risk of extinction under the increased poaching scenarios (17 and 93% for 20 and 30 individuals poached each year respectively) (Table 5). The probabilities of extinction for African wild dog and cheetah under the disease scenario would be 7 and 12% respectively. Under the Bigger and Connected, but Cheap, Reserve scenario the leopard metapopulation would still be at high risk of extinction (78%) under high levels of poaching for skins (30 individuals poached per year) (Table 5). Under the Biggest Reserve scenario, the probability of extinction for all species did not vary significantly compared to the Bigger and Connected, but Cheap, Reserve scenario (Kolmogorov-Smirnov test statistic D; Tables 5 and 6).
According to the sensitivity analyses, increasing carrying capacity was the most important factor decreasing the probability of extinction for African wild dog, cheetah and lion, whilst increased severity of poaching was the most important factor increasing the metapopulation probability of extinction for black rhino, elephant and leopard (Figure 4). Changes in other parameters did not affect the probability of extinction significantly.
Other parameters did not affect the probability of extinction significantly and were not included.
Overall, the results of the multi-criteria decision analysis suggest that the Bigger and Connected, but Cheap, Reserve scenarios were the most effective in reducing the metapopulation probability of extinction for all species, whilst the Small Reserve scenarios were the least effective (Table 6). Overall, larger and better connected protected areas were found to be a good compromise for managing black rhino, cheetah, elephant and lion, which are constrained by electric fences, and African wild dog and leopard, which are unconstrained by electric fences.
We used spatially explicit metapopulation models in combination with multi-criteria decision analysis to calculate the rank of six alternative reserve scenarios with respect to their effect on the persistence of big game metapopulations. Generally, combining the two methods is useful when dealing with uncertainty . Overall, the results suggest that current management strategies are unlikely to enhance metapopulation persistence should catastrophic events affect populations in the future. Creating larger, better connected, protected areas would ensure that threats can be better mitigated in the future.
Understanding metapopulation dynamics of large, wide-ranging, mammal species is strategic to enhance their persistence in increasingly human-dominated landscapes . Overall, our results highlight how large, wide-ranging, mammal species may experience lower extinction risks in better connected reserve networks because of increased re-colonization rates. However, the results also highlight how large, wide-ranging, mammal species, which may not be able to disperse naturally, will face increased extinction risk in smaller patches. A number of studies have highlighted the importance of maintaining connectivity and creating corridors for dispersal to enhance gene flow among populations of wide-ranging species –. However, the reality is that corridors are often poorly planned because of weak theoretical and empirical bases or the habitat selected for corridor creation is unsuitable . In absence of natural dispersal, our results demonstrate the importance of maintaining larger protected areas in combination with artificial management to enhance the persistence of large, wide-ranging, mammal species . Increased poaching levels and/or other catastrophic events (see e.g. disease spread) affecting small populations simultaneously, in fact, may result in quick population declines and extinctions . In the study area, managing populations of wide-ranging mammals in larger and better connected, but fenced, protected areas would enable managers to mitigate threats and help them meet broader conservation objectives for both wide-ranging species that can disperse naturally and those that are constrained by electric fences , , . Finally, our results also highlight how translocation can be an important management strategy to enhance gene flow among populations when creating corridors for natural dispersal may not be an option , .
In the short term, the first step to create larger and better connected protected areas would be to drop internal fences between neighbouring reserves to create larger conservancies and use smaller reserves to enhance connectivity among larger patches (Small & Big Connected Reserve scenario) . Dropping internal fences would allow creating source-sink dynamics and species would re-colonize empty suitable patches naturally through dispersal, thus enhancing gene flow . This would be particularly important to stabilize elephant numbers and thus release pressure on other species . Furthermore, removing internal fences would enable including sites that are currently under the minimum 50 black rhino carrying capacity threshold for re-introduction . Again, this would be highly beneficial to improve population performance and decrease pressure on resources in very small reserves. As our results show, maintaining smaller reserves that facilitate inter-large-patch migration can decrease the risk of extinction for African wild dog and leopard, which cannot be constrained by fences , , more than simply creating larger protected areas . Recent evidence confirms that leopard and African wild dog are dispersing naturally through the landscape and have successfully moved from one reserve to the other , , .
The second step, instead, will require merging the most-connected large and small patches by using already identified linkages  in order to create larger conservancies (Big & Connected Reserve scenario). This will be particularly beneficial for the carnivore species, which are currently heavily managed with little hope to address urgent conservation issues of the species . In the long term, expanding the current protected area network to suitable habitat currently unprotected (Bigger and Connected, but Cheap, Reserve scenario) will be the most effective strategy to decrease extinction risk and stabilize numbers of all species. This is particularly so for African wild dog, cheetah, and lion. However, increased levels of poaching for animal parts could still be very detrimental for leopard, black rhino and elephant even under the Bigger and Connected, but Cheap, Reserve scenario. Mitigating these threats in the future, by reducing the demand, may require developing innovative and at the same time controversial initiatives, such as legalizing the trade in rhino horn and ivory ,  or promoting the use of alternative and affordable faux leopard skins .
Establishing such a protected area network requires long-term political, social and financial commitments that go far beyond simply declaring new parks . Strategically, partnerships between conservation agencies, the state, non-governmental organizations, private landowners and communities are now needed to make sure internal fences are dropped , . An obvious reason why such partnerships may be beneficial to all stakeholders is that, based on previous studies, management costs per unit area will generally decrease rapidly, as economies of scale mean that protected area mergers can achieve considerable cost savings , . This is not trivial at a time when protected area budgets in South Africa are soaring due to increasing protection costs of rhinos . Furthermore, targeted incentives, such as tax cuts, and financial support from local and international donors would make such initiatives more appealing and may increase support for carnivore conservation among private and communal landowners . In addition, decision-makers may consider developing new policies under which protected area size would need to be larger than a species-specific threshold. In the case of black rhino, for instance, a strict policy on protected area size for re-introduction has encouraged landowners to drop internal fences and create larger conservancies .
Acquiring land for protected area expansion is not an option in the study area because of both limited financial resources and unwillingness to sell of both local communities and private landowners . However, conservation stewardship agreements can be promoted – and are supported by local decision-makers, as a means to increase economic prosperity of communities and private landowners through conservation businesses by joining the existing protected area network . Conservation businesses based on ecotourism and sustainable resource use can potentially provide important incentives to strengthen the partnerships we mention above, whereby larger conservation areas can share management costs and maximize profit, by increasing sustainable harvesting quotas from larger wildlife populations and number of tourists visiting the area . However, in the case of newly-established conservation businesses that would join already existing protected areas (Bigger and Connected, but Cheap, Reserve scenario), it would require capital investments worth more than 2200 USD km−2 and management costs in the range of 500 to 4500 USD km−2 yr−1 to make operations sustainable , , . The lack of funding, as well as capacity for business development, in local stakeholders – especially poor communities - represents the biggest limitation to the implementation of this conservation plan. However, well-established and better capitalized private companies and tour operators could join forces with local communities and run conservation businesses on leased land, as such businesses are increasingly delivering financial benefits and guaranteeing employment to local communities , . The establishment and development of conservation businesses on communal land will also receive support at high political levels and help meet human and economic development objectives, as well as broader biodiversity objectives .
In conclusion, using population viability analysis in combination with decision-analysis, as an alternative to traditional conservation planning, can help deal with species-specific needs for the amount and spatial configuration of protected areas. Our results suggest that having a network of larger protected areas connected either through dispersal or translocation, where fences prevent conflict with humans from occurring, may represent the most effective way to mitigate threats and maintain viable metapopulations of wide-ranging species in human-dominated landscapes. Particularly, as technologies for the translocation of such species are well-verified , , , allocating resources for continued artificial management between larger protected areas may be more beneficial than allocating unsuitable habitat for corridors. We suggest such results, especially in regarding facilitating connectivity, also have broader implications that can inform endangered megafauna management, especially in the context of restoration in increasingly human-dominated landscapes .
Supplementary Methods and Results.
Research was carried out with the permission of Ezemvelo KZN Wildlife and the iSimangaliso Wetland Park Authority. The following organisations provided access to data on species occurrence and game count estimates: Ezemvelo KZN Wildlife and University of KwaZulu-Natal. We thank S. Morgan, J. Craigie, C. Kelly, G. Shannon, G. Clinning, T. Conway, K. Odendaal, S. Naylor, D. Gilroy, C. Senekal, P. Ngwenya, N. Tredger for providing occurrence data and game count estimates.
Conceived and designed the experiments: EDM PSG RS. Analyzed the data: EDM LTBH GAB RJS PSG. Wrote the paper: EDM RS. Provided inputs to manuscript: EDM LTBH GAB RJS PSG RS.
- 1. Bottrill MC, Joseph LN, Carwardine J, Bode M, Cook C, et al. (2008) Is conservation triage just smart decision making? Trends in Ecology & Evolution 23: 649–654. doi: 10.1016/j.tree.2008.07.007
- 2. Joseph LN, Maloney RF, Possingham HP (2008) Optimal allocation of resources among threatened species: a project prioritization protocol. Conservation Biology 23: 328–338. doi: 10.1111/j.1523-1739.2008.01124.x
- 3. Burgman M, Lindenmayer DB, Elith J (2005) Managing landscapes for conservation under uncertainty. Ecology 86: 2007–2017. doi: 10.1890/04-0906
- 4. Nicholson E, Possingham HP (2007) Making conservation decisions under uncertainty for the persistence of multiple species. Ecological Applications 17: 251–265. doi: 10.1890/1051-0761(2007)017[0251:mcduuf]2.0.co;2
- 5. Di Minin E, Griffiths R (2011) Viability analysis of a threatened amphibian population: modelling the past, present and future. Ecography 34: 162–169. doi: 10.1111/j.1600-0587.2010.06263.x
- 6. Fagan WF, Meir E, Prendergast J, Karieva P (2001) Characterizing population vulnerability for 758 species. Ecology Letters 4: 132–138. doi: 10.1046/j.1461-0248.2001.00206.x
- 7. Regan HM, Colyvan M, Burgman MA (2002) A taxonomy and treatment of uncertainty for ecology and conservation biology. Ecological Applications 12: 618–628. doi: 10.1890/1051-0761(2002)012[0618:atatou]2.0.co;2
- 8. Beissinger SR, Nicholson E, Possingham HP (2002) Application of Population Viability Analysis to Landscape Conservation Planning. In: Millspaugh, JJ, Thompson, FR (Eds.). Models for planning wildlife conservation in large landscapes. Elsevier Science, Netherlands, 33–49.
- 9. Nicholson E, Westphal MI, Frank K, Rochester W, Pressey RL, et al. (2006) A new method for conservation planning for the persistence of multiple species. Ecology Letters 9: 1049–1060. doi: 10.1111/j.1461-0248.2006.00956.x
- 10. Moilanen A, Cabeza M (2002) Single-species dynamic site selection. Ecological Applications 12: 913–926. doi: 10.1890/1051-0761(2002)012[0913:ssdss]2.0.co;2
- 11. Noss RF, Carroll C, Vance-Borland K (2002) A multicriteria assessment of the irreplaceability and vulnerability of sites in the Greater Yellowstone ecosystem. Conservation Biology 16: 895–908. doi: 10.1046/j.1523-1739.2002.01405.x
- 12. Carroll C, Noss RF, Paquet PC, Schumaker NH (2003) Use of population viability analysis and reserve selection algorithms in regional conservation plans. Ecological Applications 13: 1773–1789. doi: 10.1890/02-5195
- 13. Burgman MA, Possingham HP, Lynch AJJ, Keith DA, Mccarthy MA, et al. (2001) A method for setting the size of plant conservation target areas. Conservation Biology 15: 603–616. doi: 10.1046/j.1523-1739.2001.015003603.x
- 14. Cowling R, Pressey R, Rouget M, Lombard A (2003) A conservation plan for a global biodiversity hotspot–the Cape Floristic Region, South Africa. Biological Conservation 112: 191–216. doi: 10.1016/s0006-3207(02)00425-1
- 15. Nalle DJ, Montgomery C, Arthur JL, Polasky S, Schumaker NH (2004) Modeling joint production of wildlife and timber. Journal of Environmental Economics and Management 48: 997–1017. doi: 10.1016/j.jeem.2004.01.001
- 16. Haight RG, Travis LE (2007) Reserve design to maximize species persistence. Environmental Modeling & Assessment 13: 243–253. doi: 10.1007/s10666-007-9088-4
- 17. Drechsler M, Burgman MA (2004) Combining population viability analysis with decision analysis. Biodiversity and Conservation 13: 115–139. doi: 10.1023/b:bioc.0000004315.09433.f6
- 18. Noon BR, Murphy DD, Beissinger SR, Shaffer ML, Dellasala D (2003) Conservation planning for US national forests: conducting comprehensive biodiversity assessments. BioScience 53: 1217–1220. doi: 10.1641/0006-3568(2003)053[1217:cpfunf]2.0.co;2
- 19. Drechsler M, Frank K, Hanski I, O’Hara RB, Wissel C (2003) Ranking metapopulation extinction risk: from patterns in data to conservation management decisions. Ecological Applications 13: 990–998. doi: 10.1890/1051-0761(2003)13[990:rmerfp]2.0.co;2
- 20. Root KV, Akçakaya HR, Ginzburg L (2003) A Multispecies Approach to Ecological Valuation and Conservation. Conservation Biology 17: 196–206. doi: 10.1046/j.1523-1739.2003.00447.x
- 21. Steenkamp Y, Van Wyk B, Victor J, Hoare D, Smith G, et al.. (2004) Maputaland-Pondoland-Albany. In: Mittermeier RA, Robles-Gil P, Hoffman M, Pilgrim JD, Brooks T, et al.., editors. Hotspots revisited: earth’s biologically richest and most endangered ecoregions. Mexico City: Cemex. 219–228.
- 22. McCracken DP (2008) Saving the Zululand wilderness, an early struggle for nature conservation. Johannesburg, South Africa: Jacana Publishers.
- 23. Emslie RH, Amin R, Kock R (2009) Guidelines for the in situ re-introduction and translocation of African and Asian rhinoceros. Emslie RH, Amin R, Kock R, editors Gland, Switzerland: IUCN. Available: http://www.iucn.org/dbtw-wpd/html/SSC-OP-039/cover.html. Accessed 05 July 2013.
- 24. Davies-Mostert H, Mills M, MacDonald D (2009) South Africa’s wild dog Lycaon pictus meta-population management programme. In: Hayward MW, Somers MJ, editors. The reintroduction of top-order predators. Oxford: Blackwell Publishing.
- 25. Slotow R, Garaï ME, Reilly B, Page B, Carr RD (2005) Population dynamics of elephants re-introduced to small fenced reserves in South Africa. South African Journal of Wildlife Research 35: 23–32.
- 26. Hunter LTB, Pretorius K, Carlisle LC, Rickelton M, Walker C, et al. (2007) Restoring lions Panthera leo to northern KwaZulu-Natal, South Africa: short-term biological and technical success but equivocal long-term conservation. Oryx 41: 196–204. doi: 10.1017/s003060530700172x
- 27. Gusset M, Ryan SJ, Hofmeyr M, Dyk GV, Harriet T, et al. (2008) Efforts going to the dogs? Evaluating attempts to re-introduce endangered wild dogs in South Africa. Journal of Applied Ecology 45: 100–108. doi: 10.1111/j.1365-2664.2007.01357.x
- 28. Akçakaya HR, Mills G, Doncaster CP (2007) The Role of metapopulations in conservation. In: Macdonald, D.W., Service, K. (Eds.), Key topics in conservation biology. Blackwell Publishing, Oxford, 64–84.
- 29. Lindsey P, Tambling CJ, Brummer R, Davies-Mostert H, Hayward M, et al. (2011) Minimum prey and area requirements of the Vulnerable cheetah Acinonyx jubatus: implications for reintroduction and management of the species in South Africa. Oryx 45: 587–599. doi: 10.1017/s003060531000150x
- 30. IUCN (2008) IUCN red list of threatened species. Gland, Switzerland: IUCN. Available: http://www.iucnredlist.org. Accessed 05 July 2013.
- 31. Department of Environmental Affairs and Tourism (DEAT) (2007) Environmental Management: Biodiversity Act. Lists of critically endangered, endangered, vulnerable and protected species. Government Gazette, Pretoria, RSA.
- 32. Di Minin E, Fraser I, Slotow R, MacMillan DC (2013) Understanding heterogeneous preference of tourists for big game species: implications for conservation and management. Animal Conservation 16: 249–258. doi: 10.1111/j.1469-1795.2012.00595.x
- 33. Di Minin E, MacMillan DC, Goodman PS, Escott B, Slotow R, et al.. (2013) Conservation businesses and conservation planning in a biological diversity hotspot. Conservation Biology 00: n/a–n/a. doi:10.1111/cobi.12048.
- 34. Hayward MW, Kerley GIH (2009) Fencing for conservation: restriction of evolutionary potential or a riposte to threatening processes? Biological Conservation 142: 1–13. doi: 10.1016/j.biocon.2008.09.022
- 35. Balme GA, Slotow R, Hunter LTB (2009) Impact of conservation interventions on the dynamics and persistence of a persecuted leopard (Panthera pardus) population. Biological Conservation 11: 2681–2690. doi: 10.1016/j.biocon.2009.06.020
- 36. Akçakaya HR (2005) RAMAS GIS: Linking Spatial Data with Population Viability Analysis. Version 5.0. Applied Biomathematics, New York.
- 37. Somers MJ, Graf J, Szykman M, Slotow R, Gusset M (2008) Dynamics of a small re-introduced population of wild dogs over 25 years: Allee effects and the implications of sociality for endangered species’ recovery. Oecologia 158: 239–247. doi: 10.1007/s00442-008-1134-7
- 38. Bach LA, Pedersen RBF, Hayward M, Stagegaard J, Loeschcke V, et al. (2010) Assessing re-introductions of the African Wild dog (Lycaon pictus) in the Limpopo Valley Conservancy, South Africa, using the stochastic simulation program VORTEX. Journal for Nature Conservation 18: 237–246. doi: 10.1016/j.jnc.2009.09.001
- 39. Goodman PS, Conway AJ (2010) KwaZulu-Natal biodiversity status assessment report. Biodiversity asset: black rhinoceros (Diceros bicornis minor). Ezemvelo KwaZulu-Natal Wildlife, Pietermaritzburg.
- 40. Goodman PS, Craigie J, Blok R (2010) KwaZulu-Natal biodiversity status assessment report. Biodiversity asset: elephant (Loxodonta africana). Ezemvelo KwaZulu-Natal Wildlife, Pietermaritzburg.
- 41. Goodman PS, Craigie J (2010) KwaZulu-Natal biodiversity status assessment report. Biodiversity asset: leopard (Panthera pardus). Ezemvelo KwaZulu-Natal Wildlife, Pietermaritzburg.
- 42. Goodman PS, Whittington-Jones B, Craigie J (2010) KwaZulu-Natal biodiversity status assessment report. Biodiversity asset: African wild dog (Lycaon pictus). Ezemvelo KwaZulu-Natal Wildlife, Pietermaritzburg.
- 43. Phillips SJ, Anderson RP, Schapire RE (2006) Maximum entropy modeling of species geographic distributions. Ecological Modelling 190: 231–259. doi: 10.1016/j.ecolmodel.2005.03.026
- 44. Elith J, Graham CH, Anderson RP, Dudık M, Ferrier S, et al. (2006) Novel methods improve prediction of species ’ distributions from occurrence data. Ecography 2: 129–151. doi: 10.1111/j.2006.0906-7590.04596.x
- 45. Morgan S, Mackey RL, Slotow R (2008) A priori valuation of land use for the conservation of black rhinoceros (Diceros bicornis). Biological Conservation 142: 384–393. doi: 10.1016/j.biocon.2008.10.033
- 46. Wintle BA, Kavanagh RP, McCarthy MA, Burgman M (2006) Estimating and dealing with detectability in occupancy surveys for forest owls and arboreal marsupials. Journal of Wildlife Management 69: 905–917. doi: 10.2193/0022-541x(2005)069[0905:eadwdi]2.0.co;2
- 47. Blach-Overgaard A, Svenning J, Dransfield J, Greve M, Balslev H (2010) Determinants of palm species distributions across Africa: the relative roles of climate, non-climatic environmental factors, and spatial constraints. Ecography 33: 380–391. doi: 10.1111/j.1600-0587.2010.06273.x
- 48. R Core Development Tem (2011) R: A language and environment for statistical computing.
- 49. Consortium for Spatial Information website. Shuttle Radar Topographic Mission 90m Digital Elevation Data. Available: http://srtm.csi.cgiar.org. Accessed 2013 July 5.
- 50. Batjes NH (2004) SOTER-based soil parameter estimates for Southern Africa. Report 2004/04. ISRIC – World soil information, Wageningen.
- 51. ESRI (2009) ArcEditor 9.2. Redlands: ESRI.
- 52. Pettorelli N, Vik JO, Mysterud A, Gaillard J-M, Tucker CJ, et al. (2005) Using the satellite-derived NDVI to assess ecological responses to environmental change. Trends in Ecology & Evolution 20: 503–510. doi: 10.1016/j.tree.2005.05.011
- 53. Baret F, Bartholomé E, Bicheron P, Borstlap G, Bydekerke L, et al.. (2006) VGT4Africa user manual. Joint Research Centre of the European Commission, Ispra.
- 54. Laliberte AS, Ripple WJ (2004) Range contractions of North American carnivores and ungulates. BioScience 54: 123–138. doi: 10.1641/0006-3568(2004)054[0123:rconac]2.0.co;2
- 55. Ezemvelo KwaZulu-Natal Wildlife (2008) KwaZulu-Natal Land Cover 2008 V1.1. Ezemvelo KwaZulu-Natal Wildlife, Pietermaritzburg.
- 56. Guisan A, Graham CH, Elith J, Huettmann F (2007) Sensitivity of predictive species distribution models to change in grain size. Diversity and Distributions 13: 332–340. doi: 10.1111/j.1472-4642.2007.00342.x
- 57. Davies-Mostert HT, Kamler JF, Mills GL, Jackson CR, Rasmussen GSA, et al. (2012) Long-distance transboundary dispersal of African wild dogs among protected areas in southern Africa. African Journal of Ecology 50: 500–506. doi: 10.1111/j.1365-2028.2012.01335.x
- 58. Hayward MW, Adendorff J, Moolman L, Hayward GJ, Kerley GIH (2007) The successful reintroduction of leopard Panthera pardus to the Addo Elephant National Park. African Journal of Ecology 45: 103–104. doi: 10.1111/j.1365-2028.2006.00673.x
- 59. Hayward M, Obrien J, Kerley G (2007) Carrying capacity of large African predators: predictions and tests. Biological Conservation 139: 219–229. doi: 10.1016/j.biocon.2007.06.018
- 60. Stuart C, Stuart T (2000) Field guide to the larger mammals of Africa. Cape Town: Struik.
- 61. East R (1984) Rainfall, soil nutrient status and biomass of large African savanna mammals. African Journal of Ecology 22: 245–270. doi: 10.1111/j.1365-2028.1984.tb00700.x
- 62. Jooste E, Hayward MW, Pitman RT, Swanepoel LH (2013) Effect of prey mass and selection on predator carrying capacity estimates. European Journal of Wildlife Research. doi:10.1007/s10344-013-0696-9.
- 63. Akçakaya HR (2000) Viability analyses with habitat-based metapopulation models. Population Ecology 42: 45–53. doi: 10.1007/s101440050008
- 64. Elmhagen B, Angerbjörn A (2001) The applicability of metapopulation theory to large mammals. Oikos 94: 89–100. doi: 10.1034/j.1600-0706.2001.11316.x
- 65. Rabinowitz A, Zeller KA (2010) A range-wide model of landscape connectivity and conservation for the jaguar, Panthera onca. Biological Conservation 143: 939–945. doi: 10.1016/j.biocon.2010.01.002
- 66. Linkie M, Chapron G, Martyr DJ, Holden J, Leader-Williams N (2006) Assessing the viability of tiger subpopulations in a fragmented landscape. Journal of Applied Ecology 43: 576–586. doi: 10.1111/j.1365-2664.2006.01153.x
- 67. Wikramanayake E, Knight MMC, Dinerstein E, Joshi A, Gurung B, et al. (2004) Designing a conservation landscape for tigers in human-dominated environments. Conservation Biology 18: 839–844. doi: 10.1111/j.1523-1739.2004.00145.x
- 68. Simberloff D, Farr JA, Cox J, Mehlman DW, Farr JA, et al. (1992) Movement corridors: conservation bargains or poor investments? Conservation Biology 6: 493–504. doi: 10.1046/j.1523-1739.1992.06040493.x
- 69. Parks SA, Harcourt AH (2002) Reserve size, local human density, and mammalian extinctions in U.S. protected areas. Conservation Biology 16: 800–808. doi: 10.1046/j.1523-1739.2002.00288.x
- 70. Hess GR (1994) Conservation corridors and contagious disease: a cautionary note. Conservation Biology 8: 256–262. doi: 10.1046/j.1523-1739.1994.08010256.x
- 71. Burkey TV (1989) Extinction in nature reserves: the effect of fragmentation and the importance of migration between reserve segments. Oikos 55: 75–81. doi: 10.2307/3565875
- 72. Packer C, Loveridge A, Canney S, Caro T, Garnett ST, et al. (2013) Conserving large carnivores: dollars and fence. Ecology Letters 16: 635–641. doi: 10.1111/ele.12091
- 73. Trinkel M, Ferguson N, Reid A, Reid C, Somers M, et al. (2008) Translocating lions into an inbred lion population in the Hluhluwe-iMfolozi Park, South Africa. Animal Conservation 11: 138–143. doi: 10.1111/j.1469-1795.2008.00163.x
- 74. Lindsey PA, Romañach SS, Davies-Mostert HT (2009) The importance of conservancies for enhancing the value of game ranch land for large mammal conservation in southern Africa. Journal of Zoology 277: 99–105. doi: 10.1111/j.1469-7998.2008.00529.x
- 75. Thomas CD, Kunin W (2000) The spatial structure of populations. Journal of Animal Ecology 6: 647–657. doi: 10.1046/j.1365-2656.1999.00330.x
- 76. Aarde RJV, Jackson TP (2006) Megaparks for metapopulations: addressing the causes of locally high elephant numbers in southern Africa. Biological Conservation 134: 289–297. doi: 10.1016/j.biocon.2006.08.027
- 77. Maddock A, Benn GA (2013) Identification of conservation-worthy areas in Northern Zululand, South Africa. Conservation Biology 14: 155–166. doi: 10.1046/j.1523-1739.2000.98241.x
- 78. Milliken BT, Shaw J, Emslie RH, Taylor RD, Turton C (2012) The South Africa – Viet Nam rhino horn trade nexus. Johannesburg, South Africa: Traffic. Available: http://www.trafficj.org/publication/12_The_SouthAfrica-VietNam_RhinoHorn_Trade_Nexus.pdf. Accessed 05 July 2013.
- 79. van Kooten GC (2008) Protecting the African elephant: a dynamic bioeconomic model of ivory trade. Biological Conservation 141: 2012–2022. doi: 10.1016/j.biocon.2008.05.016
- 80. Balme GA, Lindsey PA, Swanepoel LH, Hunter LTB (2013) Failure of research to address the range wide conservation needs of large carnivores: leopards in South Africa as a case study. Conservation Letters 00: n/a–n/a. doi:10.1111/conl.12028.
- 81. Bruner AG, Gullison RE, Balmford A (2004) Financial costs and shortfalls of managing and expanding protected-area systems in developing countries. BioScience 54: 1119–1126. doi: 10.1641/0006-3568(2004)054[1119:fcasom]2.0.co;2
- 82. Di Minin E, Fraser I, Slotow R, MacMillan DC (2013) Conservation marketing and education for less charismatic biodiversity and conservation businesses for sustainable development. Animal Conservation 16: 263–264. doi: 10.1111/acv.12060
- 83. Balmford A, Gravestock P, Hockley N, McClean CJ, Roberts CM (2004) The worldwide costs of marine protected areas. Proceedings of the National Academy of Sciences of the United States of America 101: 9694–9697. doi: 10.1073/pnas.0403239101
- 84. Downsborough L, Shackleton CM, Knight AT (2011) The potential for voluntary instruments to achieve conservation planning goals: the case of conservancies in South Africa. Oryx 45: 357–364. doi: 10.1017/s0030605310001559
- 85. Knight AT, Grantham HS, Smith RJ, McGregor GK, Possingham HP, et al. (2011) Land managers’ willingness-to-sell defines conservation opportunity for protected area expansion. Biological Conservation 144: 2623–2630. doi: 10.1016/j.biocon.2011.07.013
- 86. Wensing D (2006) A baseline economic study of the Gumbi Community Reserve “Somkhanda.” WWF South Africa, Johannesburg.
- 87. Snyman SL (2012) The role of tourism employment in poverty reduction and community perceptions of conservation and tourism in southern Africa. Journal of Sustainable Tourism 20: 395–416. doi: 10.1080/09669582.2012.657202
- 88. Gusset M, Jakoby O, Müller MS, Somers MJ, Slotow R, et al. (2009) Dogs on the catwalk: modelling re-introduction and translocation of endangered wild dogs in South Africa. Biological Conservation 142: 2774–2781. doi: 10.1016/j.biocon.2009.07.007
- 89. Nilsen EB, Milner-Gulland EJ, Schofield L, Mysterud A, Stenseth NC, et al. (2007) Wolf reintroduction to Scotland: public attitudes and consequences for red deer management. Proceedings of the Royal Society B: Biological Sciences 274: 995–1003. doi: 10.1098/rspb.2006.0369