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Functional Gene Polymorphism to Reveal Species History: The Case of the CRTISO Gene in Cultivated Carrots

  • Vanessa Soufflet-Freslon ,

    Contributed equally to this work with: Vanessa Soufflet-Freslon, Matthieu Jourdan

    Affiliations Agrocampus Ouest, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Angers, France, Université d’Angers, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Angers, France, INRA, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Beaucouzé, France

  • Matthieu Jourdan ,

    Contributed equally to this work with: Vanessa Soufflet-Freslon, Matthieu Jourdan

    Affiliations Agrocampus Ouest, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Angers, France, Université d’Angers, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Angers, France, INRA, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Beaucouzé, France

  • Jérémy Clotault,

    Affiliations Agrocampus Ouest, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Angers, France, Université d’Angers, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Angers, France, INRA, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Beaucouzé, France

  • Sébastien Huet,

    Affiliations Agrocampus Ouest, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Angers, France, Université d’Angers, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Angers, France, INRA, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Beaucouzé, France

  • Mathilde Briard,

    Affiliations Agrocampus Ouest, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Angers, France, Université d’Angers, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Angers, France, INRA, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Beaucouzé, France

  • Didier Peltier,

    Affiliations Agrocampus Ouest, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Angers, France, Université d’Angers, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Angers, France, INRA, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Beaucouzé, France

  • Emmanuel Geoffriau

    emmanuel.geoffriau@agrocampus-ouest.fr

    Affiliations Agrocampus Ouest, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Angers, France, Université d’Angers, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Angers, France, INRA, UMR1345 Institut de Recherche en Horticulture et Semences, SFR4207 QUASAV, Beaucouzé, France

Functional Gene Polymorphism to Reveal Species History: The Case of the CRTISO Gene in Cultivated Carrots

  • Vanessa Soufflet-Freslon, 
  • Matthieu Jourdan, 
  • Jérémy Clotault, 
  • Sébastien Huet, 
  • Mathilde Briard, 
  • Didier Peltier, 
  • Emmanuel Geoffriau
PLOS
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Abstract

Background

Carrot is a vegetable cultivated worldwide for the consumption of its root. Historical data indicate that root colour has been differentially selected over time and according to geographical areas. Root pigmentation depends on the relative proportion of different carotenoids for the white, yellow, orange and red types but only internally for the purple one. The genetic control for root carotenoid content might be partially associated with carotenoid biosynthetic genes. Carotenoid isomerase (CRTISO) has emerged as a regulatory step in the carotenoid biosynthesis pathway and could be a good candidate to show how a metabolic pathway gene reflects a species genetic history.

Methodology/Principal Findings

In this study, the nucleotide polymorphism and the linkage disequilibrium among the complete CRTISO sequence, and the deviation from neutral expectation were analysed by considering population subdivision revealed with 17 microsatellite markers. A sample of 39 accessions, which represented different geographical origins and root colours, was used. Cultivated carrot was divided into two genetic groups: one from Middle East and Asia (Eastern group), and another one mainly from Europe (Western group). The Western and Eastern genetic groups were suggested to be differentially affected by selection: a signature of balancing selection was detected within the first group whereas the second one showed no selection. A focus on orange-rooted carrots revealed that cultivars cultivated in Asia were mainly assigned to the Western group but showed CRTISO haplotypes common to Eastern carrots.

Conclusion

The carotenoid pathway CRTISO gene data proved to be complementary to neutral markers in order to bring critical insight in the cultivated carrot history. We confirmed the occurrence of two migration events since domestication. Our results showed a European background in material from Japan and Central Asia. While confirming the introduction of European carrots in Japanese resources, the history of Central Asia material remains unclear.

Introduction

Selection events along the species or breeding history generally lead to signatures of selection at the molecular level, i.e. local variations of diversity or allele frequencies at genes underlying phenotypic variations or in their surrounding region. The detection of signatures of selection encounters various issues. Firstly, demographic events like population subdivision can modify genetic polymorphism and mimic selective events [1]. Secondly, selection pattern can vary along a gene [2], [3]. Signatures of selection are therefore detected or not, according to the targeted genomic region and the linkage disequilibrium (LD; i.e., the non-random association of alleles at different loci) throughout the gene.

The history of carrot (Daucus carota subsp. sativus), cultivated worldwide, might have lead to such selection signatures. The domestication of this species is thought to have occurred in the Afghanistan region before the 900 s [4]. The first cultivated carrots were purple or yellow rooted. Their cultivation spread along trade routes, reaching Middle East and North Africa, and then to Europe in the Middle Ages. They were gradually replaced by white- and orange-rooted forms, which appeared in the 1600 s [5]. Concomitantly, a red type appeared in Asia, and particularly in Japan in the 1700 s [6], [7]. Finally, since the nineteenth century, orange-rooted carrots have spread from Europe to other continents and have become predominant commercially. Diversity analyses were recently assessed in cultivated carrot and revealed a genetic subdivision between Western (European and American) and Eastern (Asian) accessions [8], [9].

Root pigmentation depends on the relative proportion of different carotenoids, in combination with anthocyanins for the purple type [10]. Orange- and red-rooted carrots accumulate large amounts of carotenoids, mainly β- and α-carotene for the orange type or lycopene and β-carotene for the red type. Yellow-rooted carrots present low amounts of carotenoids, especially lutein and β-carotene. White-rooted carrots contain negligible amounts of carotenoids. The genetic control for root carotenoid content can be associated with carotenoid biosynthetic genes in different species including carrot [11], [12], [13]. These data suggest that the carotenoid biosynthetic genes may have been targeted by human selection.

The carotenoid biosynthesis pathway was established in higher plants [14], [15]. This pathway involves a series of desaturations, cyclisations, hydroxylations and epoxidations. Most of the carotenoid genes were identified in various species [14], [15], [16], [17], [18] including carrot [19].

Among genes, the carotenoid isomerase (CRTISO) has emerged as a regulatory step in the carotenoid biosynthesis pathway. CRTISO enzyme catalyses the cis-to-trans isomerisation reactions leading to all trans-lycopene, the substrate for the subsequent lycopene cyclisation to form all trans-α/β-carotene [20]. The identification of CRTISO as an isomerase was also confirmed by its functional expression in Escherichia coli in which it was able to convert cis-carotenoids to all trans-carotenoids [17], [18]. CRTISO mutants, such as ccr2 (Arabidopsis), phs3 (rice) and tangerine (tomato), result in the accumulation of cis-carotenoids in the dark-grown plants [17], [21] and fruits [18].

During plant evolution, paralogous genes for several carotenoid biosynthesis enzymes have been conserved and subjected to subfunctionalization: the paralogs are differentially expressed in photosynthetic or non-photosynthetic tissues [22]. A mutation affecting the paralog expressed in non-photosynthetic tissues would therefore not affect the carotenogenesis needed for photosynthesis. In most species in which carotenoid isomerase was characterized (except maize, [23]), only one gene was found (tomato [17], carrot [19], rice [24]). One hypothesis to this tendency of CRTISO to be single-copy is that CRTISO activity could be partially redundant in the light because of photoisomerisation. Indeed, light could substitute for the lack of isomerase activity in ccr2 mutants which then synthesize efficiently most carotenoids [18]. A similar light-induced isomerisation of prolycopene to all trans-lycopene was observed in the outer green tissues in immature fruit of tangerine mutants that were exposed to light but not in non-photosynthetic tissues of flowers (petals), ripe fruit, and the innermost parts of the green fruit [17]. Consequently, the function of CRTISO in plants is presumably to enable carotenoid biosynthesis to occur in the dark and in non-photosynthetic tissues such as the root, case of carrot. By comparison to other crucial steps in the carotenoid pathway, CRTISO may have evolved with fewer constraints due to photoisomerisation, and may have been more prone to be subjected to artificial selection in non-photosynthetic organs, like root. Clotault et al. [25] showed that CRTISO gene has undergone through selection events in cultivated carrot but the polymorphism pattern was observed among partial CRTISO sequence (only 700–1,000 pb). The particular status of this gene and preliminary results suggest that CRTISO gene could be a good candidate for selection signature research. The analysis of the nucleotide polymorphism and the LD among the complete CRTISO sequence will enable to clarify the selection pattern, depending on the gene structure and in relation with colour types. Moreover, selection effect can spread over genomic regions around the gene by a selective sweep. The study of the intrachromosomic LD will allow testing this hypothesis.

Our work aimed to know whether polymorphism within CRTISO, a key gene involved in the carotenoid biosynthesis pathway especially in root organs, should reflect the cultivated carrot history, including selective events during breeding. The present results showed a signature of selection pattern at the CRTISO gene and the critical contribution of CRTISO polymorphism for explaining the cultivated carrot history, especially regarding material from Asia and Central Asia.

Materials and Methods

Plant Material

Thirty nine cultivars of carrot (Daucus carota subsp. sativus) were sampled (Table 1); each one was represented by a single individual. These cultivars were chosen to maximize the diversity according to geographical origin, root colour and shape. Seeds were obtained from several seed banks and breeding companies. Genomic DNA was extracted from 50 mg of young freeze-dried leaves by using a modified CTAB protocol [26].

Microsatellite Genotyping

In order to study genetic structure of the sample, 17 microsatellite primer pairs (Table S1) were chosen based upon their reproducibility and coverage of the carrot genome [27]. Five microsatellite markers were located around CRTISO gene to investigate the linkage disequilibrium on the linkage group 4.

PCR reactions were performed in 20 µL volume with 10–50 ng genomic DNA, 1X PCR buffer, 0.2 mM dNTPs, 2 mM MgCl2, 0.25 µM of fluorescent dye-labelled forward primer, 0.25 µM of reverse primer, and 1U Taq DNA polymerase (Interchim, Montluçon, France). Amplifications were carried out by using a MyCycler thermalcycler (Biorad, Hercules, CA). The thermalcycler was programmed as follows: initial denaturation at 94°C for 2 min, 35 cycles at 94°C for 30 s, annealing temperature for 30 s, 72°C for 30 s, and a final extension at 72°C for 10 min.

The amplified fragments were analyzed by capillary electrophoresis (ABI 3730 DNA Analyzer, Applied Biosystems, Foster City, CA), and labelled PCR products were automatically sized with Genemapper 3.7 software (Applied Biosystems, Foster City, CA).

Genetic Structure

Microsatellite data were used to investigate the genetic structure of the sample with STRUCTURE 2.1 software [28]. The genetic model was used without information on the origin of each individual and allowing for admixture and allele correlated frequencies. Ten independent simulations, with a burn-in period length of 105 and a run length of 106, were used for each number of clusters (K) from one to ten. The most likely number of genetic clusters was selected using Evanno’s [29] and the highest lnP(P) methods. For each individual, the proportion q of its genome assigned to each cluster and its 95% confidence interval were calculated. An individual was assigned to a cluster if q>0.5. A CA (Correspondence Analysis) was also performed by using Genetix 4.05 software [30].

Fixation indexes were estimated among the colour groups (FCT), by using FSTAT 2.9 software [31] according to Weir and Cockerham (1984) formula [32].

Allele Sequencing

Gene-specific primers were designed and synthesized (Figure 1), based on the mRNA sequence of Daucus carota subsp. sativus (GenBank accession number DQ192188) in order to obtain the complete CRTISO sequence (exons and introns).

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Figure 1. Position of primers used for CRTISO amplification.

Boxes and lines represent exons and introns, respectively, of the CRTISO sequence. Arrows indicate partial sequences amplified with primers. The hatched box above the CRTISO sequence corresponds to the partial sequence analysed by [25].

https://doi.org/10.1371/journal.pone.0070801.g001

PCR reactions were performed in 25 µL volume with 10–50 ng genomic DNA, 1X PCR buffer, 2 mM MgCl2, 0.2 mM dNTPs, 0.2 µM of both forward and reverse primers, and 1U Taq DNA polymerase (Interchim, Montluçon, France). Amplifications were carried out by using a MyCycler thermalcycler (Biorad, Hercules, CA). The cycling conditions were: initial denaturation at 94°C for 3 min, 35 cycles at 94°C for 30 s, annealing at 55°C for 45 s, 72°C for 2 min, and a final extension at 72°C for 5 min.

PCR products were purified with ExoSAP-IT (GE Healthcare, France) and sequenced with BigDye® Terminator 3.1 chemistry (Applied Biosystems, Weiterstadt, Germany) in an ABI Prism 3730 sequencer (Applied Biosystems, Weiterstadt, Germany) according to the supplier’s instructions. The primers used to amplify PCR fragments were also used for the sequencing. Eight partial genomic regions were sequenced with forward and reverse primers to discard PCR errors (Table 2). Nucleotide sequences were read with Sequence Scanner 1.0 software (Applied Biosystems, Weiterstadt, Germany) and the credibility between the two reads was checked with Geneious 5.0.2 software [33]. For each individual, the eight partially overlapping sequences were assembled in a contiguous sequence with a program compiled in C++ [34].

The contiguous sequences of the 39 individuals were aligned with ClustalW software [35]. For heterozygous individuals, allelic phases were reconstructed by using the algorithm provided by PHASE implemented in DnaSP 5.10 software [36].

Sequence Polymorphism

The population genetic parameters were defined by using DnaSP 5.10 software [36]. Sites with alignment gaps were excluded from analysis.

The population-level genetic variation was estimated as nucleotide polymorphism (θw; [37]) and nucleotide diversity (π; [38]). θw is based on the number of segregating sites while π is based on the pairwise differences between sequences in the population. The analyses of nucleotide polymorphism (θw) and nucleotide diversity (πT) were based both upon the overall CRTISO sequence and upon a 100 bp window with a step size of 25 bp.

Haplotypes were inferred with DnaSP 5.10 software by including infinite-site violations. The number of haplotypes h and haplotype diversity Hd were then calculated [38].

A median-joining network establishing relationships among haplotypes based on the number of polymorphisms (each insertion/deletion -indel- was considered as a single nucleotide polymorphism -SNP-) was performed with Network 4.61 software [39].

Linkage Disequilibrium

Linkage disequilibrium (LD) is the non-random association of alleles at different loci. The level of LD is influenced by many factors such as population subdivision, selection, genetic linkage, recombination rate [40]. We chose to measure LD by using r2 because it summarizes both recombination and mutation histories [41].

LD was investigated by using TASSEL 3.0 software [42] not only along the complete CRTISO sequence but also along the linkage group 4 where CRTISO gene and five microsatellite markers were mapped [27]. Since rare alleles can generate a large variance in LD estimates, only biallelic loci with at least 5% frequency were used to calculate r2, which was plotted for all pairwise comparisons among SNPs. Given the influence of population subdivision, LD was calculated in each genetic group revealed by population structure analysis.

Neutrality Tests

Tajima’s D test [43], implemented in DnaSP 5.10 software [36], was used to estimate the allele frequency departure from neutral expectation, by considering the dataset as a whole, but the genetic and colour groups separately. Positive and negative D values reveal an excess of intermediate and low frequency variants, respectively. Tajima’s D test was calculated for the overall CRTISO sequence and upon a 100 bp window with a step size of 25 bp. The statistical significance for the above tests was inferred provided that the observed value was included within the 95% confidence interval of neutral distribution, which was calculated by using 10,000 coalescent simulations in DnaSP by assuming no recombination.

The significance of observed Tajima’s D values was also compared to coalescent simulations according to a demographic model. This demographic model aimed at testing the role of genetic bottleneck during domestication and subsequent population subdivision during carrot cultivation. This model included two populations, corresponding to the Eastern and Western genetic groups, assuming constant effective population sizes, NE and NW respectively. At Td generations in the past, these two populations diverged. This divergence was confounded with the end of a genetic bottleneck, started at Tb, and characterized by an effective population size Nb. Before the bottleneck, the ancestral population size was NA. In domestication modeling studies, the bottleneck intensity d = TbTd and Nb are positively correlated and define the bottleneck severity k = Nb/d [44]. We chose arbitrarily a fixed d value of 100 generations and varied Nb in order to test ten k values: 0.2, 0.5, 1, 2, 5, 7, 10, 15, 20 and 50. Other model parameters were sampled from posterior parameter distribution obtained in [25], by using the algorithm described in [45]. For each k value, 5,000 coalescent simulations were made by using ms software [46]. For each simulation, 40 sequences from East and 38 sequences from West were obtained in order to mimic the most closely the biological dataset. Tajima’s D was calculated for each simulation by using Egglib [47]. The calculation for colour groups was made by sampling as many sequences from the Eastern and the Western groups as observed in each colour group. The statistical significance for Tajima’s D was inferred provided that the observed value was included within the 95% confidence interval of simulated distribution.

Results

Population Structure

All 17 microsatellite loci were polymorphic. A total of 174 alleles were identified with a mean of 10.2 alleles per locus, and a range from two to 19 alleles per locus. Delta K method (Figure S1) suggested two genetically distinct clusters as optimal, corroborated by CA analysis (Figure S2). But the log likelihood STRUCTURE analysis supported the presence of five clusters (Figure S1). Nevertheless, the subdivision of cultivated carrot into two genetic clusters is biologically meaningful and confirmed by literature [8], [9]. Among the studied 39 individuals, 34 were assigned to a cluster with a probability higher than 0.90 and five with a lower probability from 0.68 to 0.82 (Figure 2). The first cluster contained 19 individuals, including 12 from Europe, three from Central Asia and four from Japan. These individuals showed mainly orange, white or yellow roots. In accordance with the distinction from [8] and [9], this cluster was considered as the Western genetic group. However, the assignment probability in this cluster was the lowest for three orange rooted cultivars from Japan, along with high confidence interval, which showed an intermediate status of these cultivars. In contrast, two out of three Central Asia orange rooted cultivars were significantly assigned to this first cluster. The second cluster contained 20 individuals, only sampled in Middle East or Asia, except the Swiss cultivar ‘Kuttinger’ (code 100). Most of these individuals showed red, purple or yellow roots. This second cluster was considered as the Eastern genetic group.

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Figure 2. Genetic structure of 39 carrot cultivars based on a Bayesian approach on microsatellite data and assuming two clusters.

Each individual is represented by a single vertical box broken in two coloured segments, with length proportional to each cluster assignment probability (y-axis). Bars represent the 95% confidence interval. The individuals are placed on the x-axis according to the approximate longitude of their country of origin, from France (left) to Japan (right).

https://doi.org/10.1371/journal.pone.0070801.g002

Fixation indexes among colour groups (FCT) ranged from 0.003 to 0.142 (Table 3), showing the highest differentiation between orange and purple groups.

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Table 3. Fixation indexes (FCT) among colour groups based on microsatellite data.

https://doi.org/10.1371/journal.pone.0070801.t003

Genomic Structure of the Carotenoid Isomerase in Carrot

The sequencing of CRTISO gene resulted in an alignment of 4,234 bp for 39 carrot diploid individuals.

The CRTISO gene in carrot exhibits the same genomic structure as other species (Arabidopsis, tomato, rice): these genes are split into 13 exons and 12 introns [17], [18], [24] (Figure 1). The coding and non-coding regions contributed respectively to 45 and 55% of the analysed sequences (excluding indels).

Linkage Disequilibrium

The complete CRTISO sequence exhibited a high LD level with an average r2 of 0.61 for the Western genetic group and 0.64 for the Eastern genetic group. LD showed no decay within 4,234 bp (Figure 3), when tested with the method described in [48].

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Figure 3. Linkage disequilibrium along CRTISO gene for the (A) Western and (B) Eastern genetic groups.

LD level was measured by squared correlations of allele frequencies (r2) against physical distance between pairs of SNPs.

https://doi.org/10.1371/journal.pone.0070801.g003

LD was also evaluated along LG4 [12] by using five microsatellite loci flanking CRTISO gene. Low LD level was observed between markers and CRTISO gene (Figure 4). Indeed, r2 ranged from 0 to 0.24 for both Western and Eastern genetic groups. Only GSSR6 and GSSR96, separated by 11.5 cM, were in linkage disequilibrium (r2 = 0.47) in the Western genetic group.

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Figure 4. Linkage disequilibrium along linkage group 4 for the (A) Western and (B) Eastern genetic groups.

LD level and its significance are represented at the top and at the bottom of the matrix, respectively. LD level was measured by squared correlations of allele frequencies (r2) against genetic distance. Significances of LD for microsatellite pairs are given as P-values determined by permutations. Each matrix compares the LD between pairs of markers displayed at the left and the bottom.

https://doi.org/10.1371/journal.pone.0070801.g004

Nucleotide Diversity and Neutrality Test

The observed nucleotide variation of the CRTISO genomic sequence, among the whole dataset, the Western versus Eastern genetic group, and the colour groups, is summarized in table 4.

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Table 4. Nucleotide polymorphism of CRTISO in a sample of 39 carrot cultivars.

https://doi.org/10.1371/journal.pone.0070801.t004

Within the whole dataset.

Among the studied 78 allelic sequences, 15 haplotypes were identified, among which five were found only once (singleton haplotypes). Haplotypes differed by 212 polymorphisms, consisting of 196 nucleotide substitutions and 16 indels. Among the 55 substitutions found in coding regions, 18 were non-synonymous.

The overall nucleotide diversity was moderate (πT = 0.01753). The synonymous diversity value (πsyn = 0.02788) was much higher than the non-synonymous one (πnonsyn = 0.00354), yielding a πnonsyn/πsyn ratio of 0.12697. The nucleotide diversity was higher within non-coding regions (πnon-coding = 0.02433) than within coding regions (πcoding = 0.00928) (Table 4). High diversity values were observed within most of the introns, and particularly within intron 1 of CRTISO sequence (Figure 5A).

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Figure 5. Overall nucleotide diversity (πT) (grey line), nucleotide polymorphism (θw) (dashed line), and Tajima’s D value (black line), estimated along CRTISO gene for (A) the whole dataset, the (B) Western and (C) Eastern genetic groups.

The analysis is based upon a 100 bp window with a step size of 25 bp. The exons and introns are shown as grey and white boxes, respectively. The significance for Tajima’s D is displayed at the top of each plot.

https://doi.org/10.1371/journal.pone.0070801.g005

Tajima’s D statistic detected an excess of intermediate-frequency variants in the whole dataset, which yielded a significant positive D value (D = 2.76, P<0.01). High and significant D values were also obtained within non-coding regions (Table 5, Figure 5A). These D values were significant according to demographic models with bottleneck intensity k ≥10 (Figure S3).

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Table 5. Tajima’s D value calculated for the whole dataset, the genetic and colour groups.

https://doi.org/10.1371/journal.pone.0070801.t005

Within and between groups.

Only the Western genetic group showed a similar pattern to the whole dataset for nucleotide diversity indexes and neutrality test (Tables 4 and 5; Figures 5A and 5B). The high nucleotide diversity observed within this genetic group was consistent with a balancing selection revealed by a positive and significant D value. This balancing selection hypothesis is reinforced by significance according to demographic models with k ≥7 (Figure S3).

Otherwise, the Eastern genetic group showed low nucleotide diversity (Table 4, Figure 5C) and a non significant negative Tajima’s D (Table 5).

Among the colour groups, the overall nucleotide diversity (πT) varied from 0.01105 to 0.02071, with an average value of 0.01614. The white-, orange- and yellow-rooted carrots showed globally higher nucleotide diversity than the red and purple ones (Table 4). These results were consistent with the detected selection pattern. Indeed, the yellow and orange carrots presented a positive D value for both coding and non-coding regions revealing a balancing selection, whereas the white type presented a significant one only for the non-coding region. These results were significant for demographic models with k ≥2 for yellow carrots, k ≥15 for orange carrots and k ≥7 for white carrots (Figure S3). Notice that the red and purple types showed a trend of negative Tajima’s D values even if they did not reach statistical significance (Table 5).

Relationships among Haplotypes

The haplotype network revealed four main clusters (Figure 6A). The cluster A was the most distant one with 137 substitutions from the closest cluster. Based on haplotype 2 only, it contained mainly individuals belonging to the Western genetic group with white, yellow or orange roots (Table 1 and Figure 6B). The cluster B corresponded to 11 haplotypes. It contained yellow-, red- and purple-rooted carrots (and one white) belonging to the Eastern genetic group, and orange ones belonging predominantly to the Western genetic group but cultivated in Japan or Central Asia. In this cluster, all the orange Japanese material (haplotypes 9 to 12 only) was separated from other Asian cultivars.

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Figure 6. Median joining network derived from reconstructed DNA sequence haplotype of CRTISO gene.

Haplotypes are displayed at network nodes and are symbolised by circles whose diameter is proportional to the frequency in the sample. The code number of the haplotypes is written in the circle centre. Perpendicular hashes on the lines joining haplotypes represent substitutions. When too numerous, they are displayed as slanting hashes with the number of substitutions differentiating two network nodes. The main clusters are identified by dotted boxes. (A) The black and grey proportions of each circle represent respectively the proportion of individuals from the Western and Eastern genetic groups (estimated by STRUCTURE analysis) sharing the same haplotype. (B) Colours represent the proportion of different colour groups: white, yellow, orange, red and purple.

https://doi.org/10.1371/journal.pone.0070801.g006

Finally, the clusters C (only haplotype 1) and D (haplotypes 3 and 15) corresponded only to four individuals, with white or orange roots. Three out of four individuals belonged to the Western genetic group, whereas the two clusters were overall closer to the cluster B. The clusters C and D could constitute intermediate clusters.

Discussion

Variation of Polymorphism along the Complete Gene Sequence

In the present study, both results from microsatellites and CRTISO gene showed that cultivated carrot was divided into two genetic groups: one from Asia (Eastern genetic group), and another one mainly from Europe (Western genetic group). This subdivision is consistent with previous studies about microsatellite markers or genes [8], [9] and with the historical mention of two independent migration events from the domestication centre in Afghanistan: the first one to West in the 1200 s, and the second one to East in the 1400s [5], [6]. The haplotype network of CRTISO was especially marked by the genetic subdivision between the Western and Eastern genetic groups.

No decay of linkage disequilibrium was detected within 4,234 bp for CRTISO gene. This was consistent with results obtained by [8] which did not detect any decay of LD within 700–1,000 bp for some carotenoid biosynthetic genes in cultivated carrot. This result is unexpected for an allogamous species like carrot [49]. Indeed LD is known to decay quicker in outcrossing species than in selfing species [40]. For example, LD decays within 1,500 bp in maize [48], 200 bp in Populus tremula [50] or 500 bp in ryegrass [51]. Nevertheless, different factors could explain the observed high LD level such as low recombination rate, selection, and demographic events like population bottlenecks or population subdivisions.

By considering population differentiation obtained here, LD remained high in the Western and Eastern genetic groups, suggesting the population subdivision could not explain the absence of LD decay. However, CRTISO gene was mapped on the linkage group 4 near the centromeric region [27], and some studies revealed a high LD level in centromeric regions due to low recombination rate for several species (maize [48], human [52], [53]). Carrot is a recently domesticated and biennial species: this has resulted in fewer recombination events than expected. Despite the absence of LD decay in CRTISO gene, the Tajima’s D value varied a lot along the sequence, varying from significant to non significant values, especially in the whole dataset and the Western genetic group. The partial sequence analyzed in [8] and [25] showed a similar pattern than the complete sequence. However, caution is needed when conducting candidate-genes selection analysis on partial gene sequence.

The Effect of Selection at CRTISO Gene

Deviations from neutrality expectations could be explained by selection or demographic events. The use of demographic models, considering both a moderate genetic bottleneck (k ≥2 to k ≥15, depending on the considered groups) during carrot domestication and a subsequent population subdivision between the Eastern and Western groups, confirmed the significance of observed D values. However, it should be noted that for an intense bottleneck hypothesis (k <2), the significance disappeared. Bottleneck intensity usually found for domestication ranges from k = 0.2 (Oryza sativa ssp. japonica [54]) to k = 2.45 (maize [55]). These domestication events led a significant loss of diversity in these cultivated species by comparison to their wild relatives. By comparison, carrot may have experienced a weaker domestication bottleneck because no significant difference of genetic diversity level was found in cultivated carrot by comparison to wild carrot [56]. Therefore, it is unlikely that the observed significant neutrality tests were obtained only by population size variations during domestication or by population subdivision during cultivation history. These simulations give credit to the selection hypothesis.

The possibility of photoisomerisation in leaves made us hypothesize that CRTISO gene may have evolved with less constraints and may have been more prone to artificial selection, for example for root colour. Indeed, the analysis of the complete CRTISO sequence demonstrated a significant positive Tajima’s D value, which indicates a departure from the neutral expectation. This significant positive value was observed for the whole dataset and the Western genetic group, whereas the Eastern genetic group exhibited a neutral evolution pattern. Therefore it appears that CRTISO evolved differentially in the Western and Eastern genetic groups.

Indeed, these results suggest balancing selection as the force governing CRTISO evolution in the Western genetic group, and are congruent with the high linkage disequilibrium and the high silent-site nucleotide diversity detected in this genetic group. Furthermore, the results obtained in our study are in agreement with the previous results [8], [25] based on the analysis of a partial CRTISO sequence corresponding mainly to the intron 1. By contrast, the Eastern genetic group showed no selection with a low nucleotide diversity.

One explanation for the potential balancing selection observed in the Western genetic group would be the selection for some colour types. A signature of balancing selection was found at CRTISO gene within yellow and orange carrots (D = 3.08636, P<0.001; D = 2.18514, P<0.05, respectively). Several models of selection maintaining diversity were suggested: heterozygote advantage at a locus, frequency-dependent selection, temporally or spatially heterogeneous selection [57]. The latter hypothesis is the most plausible for carrot colour selection. Carrot has been bred for new colours between the domestication of this species and the eighteenth century, and these selective events occurred in the centre of origin (Afghanistan region), in Europe and in Asia. Therefore, the breeding objectives varied temporally and spatially for carrot root colour.

However, caution is needed while interpreting the CRTISO polymorphism in yellow and orange carrots as evidence for signature of selection. Indeed, the differentiation between colour groups was considerably influenced by the subdivision between the Western and Eastern genetic groups. White carrots were mostly in the Western genetic group, four among six. Otherwise, all red and purple carrots (except the cultivar ‘Honbeni Kintoki’, code 426 unexpectedly) were assigned to the Eastern genetic group. The yellow group was half constituted by Western and Eastern individuals concordantly for microsatellite and CRTISO data, while the orange group was composed of individuals mainly assigned to the Western genetic group, with some individuals cultivated in Asia showing CRTISO haplotypes common to Eastern carrots. Therefore, these two colour groups are probably the most made of the two genetic groups. The respective effect of population subdivision, which could mimic balancing selection, and real balancing selection in these two colour types should be investigated in the future.

Selection footprint and high LD extent detected among CRTISO gene might lead to a selective sweep around this gene [58]. The low intrachromosomic LD observed in our study could not confirm this hypothesis. New markers in CRTISO genomic region could be developed thanks to carrot genome sequencing, and therefore could allow improving the study of selection events in this species.

Insights into the History of Cultivated Carrots

Neutral loci and CRTISO polymorphism analyses allowed testing the hypotheses about cultivated carrot history. Microsatellite markers gave information about the entire genome and were therefore very suitable to understand gene pool origin. The incongruity between the CRTISO haplotype network and genetic structure given by microsatellite analysis informed about genetic admixture.

Literature considers the purple and yellow carrots as the two original colour types [4], [5]. The purple type mainly found in Middle East was clearly assigned to the Eastern group, both from microsatellite and CRTISO sequence data. By comparison, the clustering of yellow cultivars half into the Western group and half into the Eastern group, whether through microsatellite or CRTISO sequence analyses, suggested that the yellow type had experienced two separate migration events, following domestication.

According to their assignment probability and large confidence interval estimated by STRUCTURE analysis, orange carrots sampled from Japan and Central Asia were assigned to the Western genetic group but with some admixture between Western and Eastern carrots whereas they belonged clearly to the Asian cluster depending on CRTISO data. This is congruent with the breeding of this colour type. Indeed some European cultivars were exported and adapted for cultivation to Japan in the 1900 s. They were used in breeding programs and crossed with Asian carrots [59]. In the same way, carrots from Central Asia might come from European cultivars that were either exported and cultivated straight in Central Asia, or went by Japan before being used in Central Asia. Two out of three were significantly assigned to the Western genetic group based on the microsatellite results, but to the Asian cluster and an intermediate one (haplotype 15) based on CRTISO data. It remains therefore unclear if Central Asia cultivars came from European material crossed locally with Eastern material, or they originated from an Eastern migration from Japan.

Conclusions

Neutral loci and CRTISO polymorphism analyses were shown to be complementary in order to recount the breeding history of cultivated carrot. Indeed, human activities (seed exchange and transport, and breeding) could affect allele diversity, which resulted in the distinction of two genetic groups and large diversity at the CRTISO gene. These results may suggest that human preferences for carrot root colour have varied depending on periods and geographical areas. It brings new insights about the history of orange carrots. The orange-rooted carrots spread from Europe to other continents including Asia. Therefore the breeding of this carrot form was adapted to different markets. In spite of nucleotide polymorphism and the distinction of several haplotype clusters within the orange carrots, corresponding individuals belonged mainly to the Western genetic group based on microsatellite data. This would confirm that the introduction of European carrots in Asian breeding programs is relatively recent, as genetic background remains common to both plant materials. The knowledge of the breeding history of this species, based both on neutral and functional loci, allows a better characterisation of plant material, and offers substantial insight to secure, manage and exploit carrot genetic resources while maximising genetic diversity.

Supporting Information

Figure S1.

Plots of (A) Delta K and (B) the log likelihood, from the STRUCTURE analysis.

https://doi.org/10.1371/journal.pone.0070801.s001

(TIF)

Figure S2.

Genetic structure of 39 carrot cultivars based on a correspondence analysis (CA) on microsatellite data. Squares and circles represent respectively individuals from the Western genetic group and individuals from the Eastern genetic group according to STRUCTURE results.

https://doi.org/10.1371/journal.pone.0070801.s002

(TIF)

Figure S3.

Significance of Tajima’s D according to the genetic bottleneck severity k, for (A) the total sequence, (B) the coding sequence and (C) the non-coding sequence. Ten values of bottleneck severity from 0.2 (most severe) to 50 (least severe) were tested. The significance was displayed for the whole dataset (black line), Western group (blue line), Eastern group (green line), white group (grey line), yellow group (yellow line), orange group (orange line), red group (red line) and purple group (purple line). The significance is shown as P-values for two-tailed Tajima’s test, with a logarithmic scale (y-axis). P-values were calculated by comparing the location of observed Tajima’s D with the simulated dataset obtained by the demographic model. In each plot, the areas with P<0.001, P<0.01 and P<0.05 are represented with red, orange and yellow backgrounds, respectively.

https://doi.org/10.1371/journal.pone.0070801.s003

(TIF)

Table S1.

Characteristics of 17 carrot microsatellite markers [27].

https://doi.org/10.1371/journal.pone.0070801.s004

(XLSX)

Acknowledgments

We would like to thank Charles Poncet, of the ‘Plateforme GENTYANE’ (UMR1095 GDEC, Clermont-Ferrand, France), for microsatellite genotyping. We wish to thank Françoise Gros and Marie-France Le Cunff, of the ‘Plateforme de Séquençage et de Génotypage’ (IFR26, Nantes, France), for sequencing PCR products. We thank Sylvain Gaillard for the sequence assembly program.

Author Contributions

Conceived and designed the experiments: VSF MJ EG DP. Performed the experiments: VSF MJ SH. Analyzed the data: VSF MJ JC. Contributed reagents/materials/analysis tools: VSF MJ SH JC. Wrote the paper: VSF MJ JC DP MB EG.

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