Diverse, distantly-related eukaryotic lineages have adapted to low-oxygen environments, and possess mitochondrion-related organelles that have lost the capacity to generate adenosine triphosphate (ATP) through oxidative phosphorylation. A subset of these organelles, hydrogenosomes, has acquired a set of characteristic ATP generation enzymes commonly found in anaerobic bacteria. The recipient of these enzymes could not have survived prior to their acquisition had it not still possessed the electron transport chain present in the ancestral mitochondrion. In the divergence of modern hydrogenosomes from mitochondria, a transitional organelle must therefore have existed that possessed both an electron transport chain and an anaerobic ATP generation pathway. Here, we report a modern analog of this organelle in the habitually aerobic opportunistic pathogen, Acanthamoeba castellanii. This organism possesses a complete set of enzymes comprising a hydrogenosome-like ATP generation pathway, each of which is predicted to be targeted to mitochondria. We have experimentally confirmed the mitochondrial localizations of key components of this pathway using tandem mass spectrometry. This evidence is the first supported by localization and proteome data of a mitochondrion possessing both an electron transport chain and hydrogenosome-like energy metabolism enzymes. Our work provides insight into the first steps that might have occurred in the course of the emergence of modern hydrogenosomes.
Citation: Leger MM, Gawryluk RMR, Gray MW, Roger AJ (2013) Evidence for a Hydrogenosomal-Type Anaerobic ATP Generation Pathway in Acanthamoeba castellanii. PLoS ONE8(9): e69532. https://doi.org/10.1371/journal.pone.0069532
Editor: Julio Francisco Turrens, University of South Alabama, United States of America
Received: November 30, 2012; Accepted: June 13, 2013; Published: September 27, 2013
Copyright: © 2013 Leger et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work and R.M.R.G. were supported by grant MOP-62809 awarded to A.J.R. by the Canadian Institutes of Health Research. M.M.L. is supported by the National Research Fund, Luxembourg, and by the Nova Scotia Health Research Foundation. R.M.R.G. was supported by a Predoctoral Scholarship from the Killam Trusts. Operating grant support to M.W.G. from the Canadian Institutes of Health Research (MOP-4124) as well as funding from the Tula Foundation is gratefully acknowledged. A.J.R. acknowledges support from the Canada Research Chairs Program, the Canadian Institute for Advanced Research Program in Integrated Microbial Biodiversity and the Tula Foundation. Preliminary sequence data was obtained from Baylor College of Medicine Human Genome Sequencing Center website at http://www.hgsc.bcm.tmc.edu. The DNA sequence of Acanthamoeba castellanii was supported by grant number U01 HG02051 from NIH/NHGRI to Richard Gibbs at the BCM-HGSC. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
The capacity to produce adenosine triphosphate (ATP) under low oxygen conditions is found throughout the eukaryote tree, in diverse, distantly-related organisms. Of the lineages of this type that have been studied, most are anaerobic or microaerobic, and possess mitochondrion-related organelles (MROs), which, although derived from mitochondria, have lost the capacity to generate ATP through oxidative phosphorylation (reviewed in ). Some of these organelles, known as hydrogenosomes, have adopted a new function in anaerobic ATP generation by acquiring a set of characteristic enzymes that are commonly found in anaerobic bacteria –. In other anaerobic/microaerobic eukaryotes with more highly reduced MROs, such as Giardia intestinalis and Entamoeba histolytica, homologous enzymes are localized in the cytosol, and the MROs of these organisms are not involved in ATP generation , . MROs have long been classified according to their role in energy metabolism, and a recent review  retains the categories of mitochondria (class 1 under the authors' classification system), hydrogenosomes (class 4) and mitosomes (class 5), while proposing new classes to formally accommodate the more diverse range of MROs now known: anaerobically functioning mitochondria that do not produce hydrogen (class 2) and mitochondria that both possess an electron transport chain and produce hydrogen (class 3).
The best-characterized hydrogenosomes are those of the obligately parasitic parabasalid Trichomonas vaginalis. In mitochondria, the first step of pyruvate catabolism involves the decarboxylation of pyruvate by the enzyme complex pyruvate dehydrogenase (PDH, EC 188.8.131.52), which produces acetyl-CoA and CO2 with the concomitant reduction of NAD+ to NADH. In the hydrogenosomes of T. vaginalis, pyruvate decarboxylation is instead carried out by pyruvate:ferredoxin oxidoreductase (PFO, EC 184.108.40.206) , . In other eukaryotes such as Cryptosporidium parvum, Neocallimastix frontalis or Mastigamoeba balamuthi, a similar function is performed by a pyruvate:NADP oxidoreductase (PNO, EC 220.127.116.11) ,  or pyruvate:formate lyase (PFL, EC 18.104.22.168) , . Electrons produced during pyruvate decarboxylation by PFO are transferred first to a [2Fe-2S] ferredoxin, and then to [FeFe]-hydrogenase (EC 22.214.171.124), which reduces protons, producing molecular hydrogen. Three maturases, HydE, HydF and HydG, are involved in the assembly and insertion of the catalytic iron-sulfur clusters in bacterial [FeFe]-hydrogenases, and homologs of these enzymes have been reported in some, though not all, eukaryotes possessing this enzyme –. Coenzyme A is transferred from the resulting acetyl-CoA to succinate by an acetate:succinate CoA transferase (ASCT, EC 126.96.36.199) , generating acetate and succinyl-CoA. The subsequent regeneration of succinate, catalyzed by the tricarboxylic acid (TCA) cycle enzyme succinate thiokinase (STK, also referred to as succinyl-CoA synthetase, EC 188.8.131.52), generates ATP through substrate-level phosphorylation . ASCTs found in eukaryotes have been classified into three subfamilies . Enzymes of subfamily 1A, found in the mitochondria of trypanosomatids  and in the hydrogenosomes of rumen ciliates , are homologous to the succinyl-CoA:3-oxoacid CoA transferases (SCOT) that are involved in ketone body degradation in metazoan mitochondria. Members of subfamily 1B have been reported in the mitochondria of acetate-producing metazoans such as Fasciola hepatica  and Artemia franciscana . Based on in silico predictions, they are also believed to be present in Blastocystis sp.  and in the mitochondria of Naegleria gruberi . Enzymes of subfamily 1C have been found in the hydrogenosomes of T. vaginalis , Blastocystis sp. , , and the chytrid fungus Neocallimastix , .
Until recently, the only aerobic eukaryotes known to possess both [FeFe]-hydrogenase and PFO were green algae such as Chlamydomonas reinhardtii and Scenedesmus spp. In these organisms, [FeFe]-hydrogenase and PFO are expressed upon exposure to anoxic conditions, and localize to the chloroplast, where they function in both anaerobic energy production and anaerobic photosynthesis –. In 2010, genes encoding an [FeFe]-hydrogenase and the three [FeFe]-hydrogenase maturases were identified in the genome of Naegleria gruberi, an aerobic heterolobosean; in silico predictions suggested that these enzymes might be mitochondrially targeted . No PFO homologs have been found in the genome of this organism.
Previous studies have attempted to clarify the origin of these enzymes in eukaryotes; these efforts have generally been hampered by the small number of eukaryotic sequences available, and by low resolution in all parts of the tree. Phylogenetic analyses of [FeFe]-hydrogenase sequences have consistently recovered more than one eukaryotic clade, suggesting at least two origins of these enzymes in eukaryotes , –. A specific relationship between eukaryotic [FeFe]-hydrogenases and their homologs in α-proteobacteria has been rejected in topology tests, providing evidence against a mitochondrial endosymbiotic origin of [FeFe]-hydrogenases in extant eukaryotes . Analyses of [FeFe]-hydrogenase maturases recovered robust eukaryotic clades in all cases; however, the internal relationships within these clades were poorly supported and their closest prokaryotic homologs were, in no case, α-proteobacterial. Similar results were obtained by phylogenetic analyses of PFO , –. A neighbor-net analysis of ASCT1B and ASCT1C sequences  recovered eukaryote monophyly for both enzymes, consisting of metazoa in the case of ASCT1B, and fungi and T. vaginalis in the case of ASCT1C; at that time these taxa were the only eukaryotes known to possess ASCTs. Again, no clear α-proteobacterial affinity for eukaryotic groups was recovered, and thus there is no clear connection to mitochondrial origins. These observations suggest that lateral gene transfer has played a role in the appearance of these enzymes within eukaryotes; however the number of events involved, and the precise nature of the donor and recipient lineages, remain unclear.
Acanthamoeba castellanii is a free-living soil amoeba, found in a diverse range of marine, freshwater, soil and human-related environments. As an opportunistic pathogen, it is responsible for amoebic keratitis and granulomatous amoebic encephalitis in humans , and under free-living conditions, it grazes on bacterial biofilms . Thus it is likely that A. castellanii routinely encounters anaerobic or microaerobic conditions. Furthermore, while this amoebozoan has been reported to encyst rapidly when exposed to degassing with N2 , it is now known to respond well to low-oxygen conditions, replicating faster under these conditions than under aerobic ones .
In 2010, Hug et al. reported partial sequences of a few enzymes associated with anaerobic ATP generation in publicly available expressed sequence tag (EST) data from A. castellanii . Here, we report the existence a complete ‘hydrogenosomal’ type anaerobic ATP generation pathway, describe the genomic and transcript sequences of all enzymes involved, and show that they all possess classical mitochondrial targeting peptides. We show, by tandem mass spectrometry, that three of these enzymes – PFO, ASCT1B, and the [FeFe]-hydrogenase maturase HydF – are found in the mitochondria of aerobically grown cells. Our findings confirm the presence of a complete hydrogenosome-like ATP generation pathway in A. castellanii and strongly suggest that the enzymes are present within the mitochondria of this organism. Our results raise the tantalizing possibility that the mitochondrion of A. castellanii is able to act as an organelle with two metabolic modes, producing energy either aerobically, via classical oxidative phosphorylation, or anaerobically, via a hydrogenosomal-type pathway, according to the environmental conditions that the amoeba encounters.
Materials and Methods
454 ESTs available through the Baylor College of Medicine Human Genome Sequencing Center (http://www.hgsc.bcm.tmc.edu/ftp-archive/AcastellaniNeff/ESTs/) were assembled using CAP3  and Mira , .
Partial EST sequences of [FeFe]-hydrogenase, PFO, HydE and HydG previously identified by Hug et al.  were retrieved by performing Basic Local Alignment Search Tool (BLAST ) searches against the publicly available A. castellanii expressed sequence tag (EST) library at TBestDB . Genomic sequence data were obtained by performing subsequent BLASTn searches against scaffolds assembled by the Baylor College of Medicine Human Genome Sequencing Center (http://www.ncbi.nlm.nih.gov/bioproject/PRJNA20303; see Table S1 for accession numbers), using the A. castellanii ESTs as queries. As no ESTs encoding HydF or an ASCT had been identified, we used a Clostridium kluyveri HydF protein sequence (EDK34342) and Trypanosoma brucei, Fasciola hepatica and Trichomonas vaginalis ASCT sequences (EAN79240, ACF06126 and XP_001330176, respectively) as heterologous query sequences for tBLASTn searches. Subsequently, full-length cDNA sequences were obtained by performing BLASTn searches against the 454 EST data, using previously identified EST or genomic sequences as queries. The identities of hits were verified by performing BLASTx searches against GenBank. Start codons and intron positions were verified manually. Predicted mitochondrial targeting peptides were identified using TargetP –.
Cells were maintained at room temperature, but otherwise as described in .
Tandem mass spectrometry
Mitochondria were isolated from aerobically grown cells, purified on sucrose gradients, and subfractionated as described in . A whole mitochondrial fraction (SWM), a soluble protein-enriched fraction (SPE), and a mitochondrial membrane protein-enriched fraction (MPE) were separated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels; the resulting lanes were excised in approximately equally sized bands and digested, as described in . These fractions were separated by reverse-phase high-performance liquid chromatography (HPLC) and subjected to tandem mass spectrometry (MS/MS) as described . An additional whole mitochondrial fraction that had not undergone SDS-PAGE was digested in solution (WM) and separated into fractions using strong cation exchange liquid chromatography; these fractions were resolved by reversed-phase HPLC and subjected to MS/MS. Data were acquired and analyzed against the genomic and 454 EST data using Mascot , as described in . Of the 143 nuDNA-encoded proteins identified by MS/MS in , representing respiratory chain proteins (88 proteins) and non-respiratory chain proteins that contaminated preparations of respiratory complexes (55 proteins), none were known non-mitochondrial contaminants and none were confidently predicted to possess sorting signals that direct proteins to other cellular compartments, such as the endoplasmic reticulum or peroxisomes; accordingly, this protocol was deemed to have produced sufficiently pure mitochondria samples. In order to confirm that the samples used in this particular experiment were highly enriched in mitochondria, we compared the gel electrophoretic profile of the rRNA species recovered from the purified mitochondria to that of total cellular RNA, as a proxy for protein profiles (Figure S1). The RNA profiles were distinct; in particular, we were unable to detect cytosolic LSU or SSU species in our purified mitochondrial samples, indicating a high degree of mitochondrial enrichment in these samples.
For each protein, NCBI databases of proteins and EST sequences were searched using BLASTp or tBLASTx respectively, and added to preexisting datasets used in  where applicable. Preliminary alignments were made using MUSCLE , , FSA  or MAFFT-L-INS-I – and trimmed using BMGE  or a script written by Dr. Daniel Gaston. Preliminary trees were made using FastTree , or RAxML (version 7.2.6 , using the Le and Gascuel [LG] model of amino acid substitution rates  with empirical amino acid frequencies and the gamma model of rate heterogeneity [PROTGAMMALGF]. Based on these initial analyses, long-branching taxa and paralogs were eliminated. In particular, a long-branching clade comprising both eukaryotic and bacterial sequences was identified in [FeFe]-hydrogenase analyses. Final analyses were performed both with and without these sequences. Initially, ASCT1C sequences were analyzed together with distantly homologous ASCT1B sequences, and distantly homologous HydE and HydF sequences were analyzed together; this was done to better identify paralogs in the face of widespread misannotation in GenBank.
Final alignments were made using MAFFT-L-INS-I, verified manually, and trimmed using BMGE. Independent maximum likelihood (ML) trees (200) and 1000 bootstrap replicates were generated in RAxML [PROTGAMMALGF] model, and bootstrap values were mapped onto the best-scoring tree. Bayesian inference posterior probabilities were calculated using PhyloBayes  under the [catfix C20] model of evolution .
We tested support for various grouping topologies using the approximately unbiased (AU) test in CONSEL . For each hypothesis tested, five ML trees were generated for a given constraint tree, using the PROTGAMMALGF model and the –g option in RAxML. Subsequent CONSEL analyses used the 1000 bootstrap trees initially produced to generate p-values for the best trees generated from the constrained RAxML analyses.
The A. castellanii genome encodes a complete anaerobic ATP generation pathway similar to that found in T. vaginalis hydrogenosomes
Hug et al.  had identified partial sequences of [FeFe]-hydrogenase, PFO, HydE and HydG in the A. castellanii transcriptome (Table S1). We have identified corresponding genomic sequences for all four genes. Searches using a Clostridium HydF sequence and ASCT sequences from Trypanosoma brucei, Fasciola hepatica and Trichomonas vaginalis as queries yielded a HydF homolog and two possible candidates for an ASCT, homologous to the T. brucei (subfamily 1A) and the F. hepatica (subfamily 1B) enzymes. The subfamily 1A enzyme of T. brucei is homologous to succinyl-CoA:3-ketoacid-CoA transferase (SCOT), an enzyme that is widespread in mammalian and fungal mitochondria, and that catalyzes the transfer of CoA from succinyl-CoA to a 3-oxoacid. The top BLASTp hits for this candidate were SCOT homologs from Polysphondylium pallidum and Dictyostelium discoideum, two other, ‘cellular slime mold’, amoebozoans that do not appear to possess anaerobic ATP generation enzymes. In contrast, the F. hepatica-type enzyme has been described in platyhelminths and arthropods, and is homologous to bacterial 4-hydroxybutyrate CoA-transferases; we were unable to find homologs of this enzyme in the Polysphondylium pallidum or Dictyostelium genome sequences available through dictyBase . Accordingly, we concluded that the F. hepatica enzyme hit was the more likely candidate to function in a pathway with the other anaerobic enzymes we had discovered. The A. castellanii genome encodes a single adrenodoxin-like [2Fe-2S] ferredoxin, homologous to eukaryotic mitochondrial ferredoxins, which may function as the electron mediator from PFO to [FeFe]-hydrogenase; and STK, which may perform dual functions in the TCA cycle and in anaerobic ATP generation.
Full-length EST sequences for each of these enzymes were retrieved from 454 pyrosequencing data, confirming the locations of spliceosomal introns in the genomic sequences. All of the genomic sequences contained canonical 5′GT-AG3′ spliceosomal introns, refuting the possibility that the genes we identified originated from bacterial contamination of the transcriptomic and genomic data (see Table S1 for the list of accession numbers). Interestingly, the genes encoding [FeFe]-hydrogenase and all three maturases are encoded within a single ∼50-kb stretch of the genome (Figure 1). Within this region, 11 other predicted genes were found that had homologs returned by BLAST searches. With the exception of two genes encoding hypothetical proteins, all of the predicted genes had significant tBLASTx hits (with an E-value≤10−3) to one of more of the amoebozoan genomes available through dictyBase. None of these additional genes has any obvious function in anaerobic respiration.
The direction of the arrows indicates the transcriptional orientation of the genes. Green, anaerobic metabolism enzyme-encoding gene located on the forward strand. Red, anaerobic metabolism enzyme-encoding gene located on the reverse strand. Gray, predicted gene encoding a product not evidently involved in anaerobic metabolism. Predicted genes annotated according to top BLAST hits in dictyBase: A. Similar to interferon-related protein PC4-like. B. Region with similarity to Dictyostelium hypothetical protein, possibly truncated at the 5′ end. C. Similar to importin beta 4. D. Low similarity to Dictyostelium vasodilator-stimulated phosphoprotein. E. Similar to RNA-binding region RNP-1 domain-containing protein. F. Similar to molybdenum cofactor synthesis protein 1. G. Similar to molybdenum cofactor synthesis protein 2. H. Region with similarity to sequences annotated as hypothetical protein in GenBank, but without tBLASTx hits in dictyBase. I. Similar to PHD Zn finger-containing protein. J. Low similarity to Dictyostelium hypothetical protein. K. Region without significant hits in either GenBank or dictyBase, but which corresponds to EST sequence and apparently contains an intron.
TargetP predicted high probabilities of mitochondrial localization, and identified putative targeting peptide cleavage sites, for all of the anaerobic ATP generation enzymes (Figure 2). The predicted mitochondrial targeting peptides (mtTPs) are rich in hydrophobic and positively charged amino acids, consistent with the amphipathic helix structure that mitochondrial targeting peptides are known to adopt . The mtTPs have arginine residues at positions −2, −3 or −10 relative to the cleavage site, as well as positively charged residues at position −8 in the latter case. Such residues are believed to be important in determining the site of targeting peptide cleavage . The composition of the predicted targeting peptides is consistent with those predicted by TargetP for other nucleus-encoded proteins known to be mitochondrially targeted in A. castellanii, such as mitochondrial malate dehydrogenase, dihydrolipoamide dehydrogenase and isocitrate dehydrogenase (data not shown).
Putative mtTPs were predicted by TargetP, and are shown underlined and bold. N-termini of bacterial and eukaryotic homologues are shown for comparison; bacterial homologues lack targeting peptides. Positively charged residues in the predicted mtTPs are shown in red; hydrophobic residues are shown in blue. Arginine residues at positions −2, −3 or −10, and hydrophobic residues at position −8, believed to be important in determining the cleavage site , are marked with an asterisk. P, TargetP mitochondrial targeting probability.
PFO, ASCT1B and the [FeFe]-hydrogenase maturase HydF are present in the mitochondrial proteome
Peptides identified in mitochondrial fractions are shown in red. mtTP cleavage sites predicted by TargetP are shown with arrows.
Thirty unique PFO-specific peptides were identified in the WM, SWM and SPE fractions, with a high ion score (1457), evidence that PFO is present at a relatively high abundance in A. castellanii mitochondria even under aerobic conditions. No PFO-specific peptides were identified in the MPE fraction; these findings are consistent with PFO in A. castellanii being a soluble matrix protein, in contrast to that of T. vaginalis, which is bound to the hydrogenosomal membrane . ASCT1B was similarly well represented in the mitochondrial proteome (ion score: 1900, 17 unique peptides).
A single peptide from the [FeFe]-hydrogenase maturase HydF was identified, as were three peptides from ferredoxin. No peptides corresponding to [FeFe]-hydrogenase, or to the two other [FeFe]-hydrogenase maturases, were recovered.
In addition, we performed immunogold labeling experiments on A. castellanii cells that had been exposed to anaerobic conditions for 6 or 24 hr, using an antibody raised against A. castellanii [FeFe]-hydrogenase (Methods S1). Antibody staining in these cells was elevated in mitochondria (approx. 2.9-fold higher than in the cytosol, and approx. 1.7-fold higher than in the nucleus), consistent with the presence of a predicted mitochondrial targeting peptide for [FeFe]-hydrogenase (Figures S2, S3, S4), although the high levels of antibody required and the degree of cross-reaction in other cellular locations suggest that optimal expression conditions remain to be established for [FeFe]-hydrogenase in A. castellanii.
Evolutionary histories of anaerobic ATP generation enzymes
Previous analyses of [FeFe]-hydrogenase phylogenies have failed to recover eukaryotes as a monophyletic clade , , , . Our results (Figure 4), which include sequences from a larger number of eukaryotic taxa than were previously available, are consistent with these findings, in that we recover at least three distinct eukaryotic clades. While our trees suffer from the same poor resolution (i.e. low bootstrap support for many branches) that has been reported in previous analyses, it is possible to conduct approximately unbiased (AU) topology tests to determine whether the data have sufficient information to reject alternative phylogenetic hypotheses using an alpha-level of 0.05 as the significance threshold. The hypotheses tested are shown in Table 1 and include tests for the monophyly of eukaryote sequences as a whole, the grouping of A. castellanii sequences with homologs from other amoebozoans, and tests for grouping of eukaryotic and/or A. castellanii sequences with α-proteobacterial sequences (as expected if they were of mitochondrial origin). These tests show, for example, that monophyly of the A. castellanii sequence with other amoebozoan homologs, such as the sequence from Mastigamoeba balamuthi or the Entamoeba histolytica sequence, can be rejected, whereas eukaryote monophyly itself cannot be rejected. Preliminary RAxML and FastTree analyses recovered one unusually long-branching eukaryotic/bacterial clade, corresponding to the major [FeFe]-hydrogenase Clade B described in Hug et al. , which includes the M. balamuthi, both Trimastix pyriformis, and one of the E. histolytica enzymes. Separate analyses were performed excluding the taxa in this clade (Figure S5). Removing this clade did not alter the overall topology enough to recover eukaryote monophyly; again, however, in AU tests using this dataset, eukaryote monophyly was not rejected.
The topology shown is the maximum likelihood (ML) tree generated by RAxML analyses; 279 sites were examined across 175 taxa. Bootstrap support values ≥50% and Bayesian posterior probabilities ≥0.5 are shown. Eukaryotes are shaded gray.
The only previous phylogenetic analysis of [FeFe]-hydrogenase maturases  was notable in that it recovered eukaryote monophyly for all three enzymes, despite this not having been the case for [FeFe]-hydrogenase itself – even accounting for the lack of known sequences of these enzymes in some eukaryotes. This observation also holds true for our analyses (Figures S6,S7, S8), despite the additional eukaryotic sequence data that have become available in the interim. Although Spironucleus vortens groups with α- and β-proteobacteria in the HydE tree (Figure S6), this position has low bootstrap support and, as with the other two maturases, topology tests (Table 1) do not reject eukaryote monophyly for HydE. In contrast with the topology tests for [FeFe]-hydrogenase, PFO and ASCT1B, a specific grouping of eukaryotes and α-proteobacteria (as expected if the enzymes were of mitochondrial origin) is not rejected by topology tests (Table 1). Within the main eukaryotic clade, low support precludes drawing conclusions about internal relationships, including that of A. castellanii; monophyly of A. castellanii and M. balamuthi is not rejected by topology tests for any of the maturases.
Previous phylogenetic analyses of PFO reached different conclusions as to the recovery of eukaryote monophyly , . Our analyses excluded a long-branching Monocercomonoides sequence that may have distorted the topology recovered by Hug et al. ; consequently, we recover eukaryotic monophyly (Figure 5), a finding consistent with that of Horner and colleagues . As in the case of [FeFe]-hydrogenase, a hypothetical Acanthamoeba+Entamoeba clade was rejected by topology tests; but an Acanthamoeba+Mastigamoeba clade was not. However, large groupings within the eukaryote clade have poor support, preventing the inference of clear internal relationships.
The topology shown is the ML tree estimated by RAxML; 954 sites were examined across 335 taxa. Bootstrap support 50% and posterior probabilities ≥0.5 are shown. Eukaryotes are shaded gray.
A previous neighbor-net analysis of ASCT1B and ASCT1C sequences  recovered a monophyletic cluster of animal sequences, representing the only eukaryotes known to possess ASCT1B-like sequences at that time. Our analyses include additional animal sequences, as well as sequences from a number of other lineages. Monophyly of all of these eukaryote sequences is rejected in topology tests (Table 1), as is the grouping of α-proteobacteria with any of the major eukaryote groups. However, both the grouping of A. castellanii with the aerobic flagellate Malawimonas jakobiformis as well as the branches separating this clade from away from opisthokonts and Blastocystis have low bootstrap support (Figure S9), and an alternate position of this organism, grouping with opisthokonts and Blastocystis, is not rejected.
Acanthamoeba castellanii is known to inhabit aerobic environments and to produce energy via oxidative phosphorylation; it possesses a mitochondrion with a functional TCA cycle and electron transport chain similar to those found in other aerobic eukaryotes , . Nevertheless, it inhabits a wide range of soil, aquatic, and man-made environments, and it is likely that it encounters low-oxygen conditions with some frequency. An organism with such a lifestyle would likely derive significant survival benefit from being able to function under a wide range of conditions, including periods of anoxia. Here, we present the first case of a complete hydrogenosome-like ATP generation pathway with predicted mitochondrial targeting in a habitually aerobic eukaryote, and show that several of its key enzymes are present in the mitochondria. Our work provides the first evidence supported by localization data for a mitochondrion possessing the metabolic components of a ‘hybrid’ organelle, which may enable it to adopt functions in oxidative phosphorylation or anaerobic metabolism according to the conditions that it encounters. The existence of such organelles in an extant organism immediately suggests a possible sequence of events in the first steps leading to the emergence of hydrogenosomes. Upon acquiring an anaerobic energy-generating pathway, a previously obligate aerobe would be able to thrive in a more diverse range of habitats, as well as surviving temporal fluctuations in oxygen levels. Subsequently, descendents of such a cell inhabiting exclusively low-oxygen environments, with a reduced need to perform oxidative phosphorylation, might lose components of the electron transport chain, as well as other mitochondrial functions (Figure 6).
A hypothetical scenario for the acquisition of anaerobic ATP generation enzymes and the subsequent emergence of extant mitochondrion-related organelles (MROs). (1) Acquisition of anaerobic energy generation enzymes. (2) Loss of the capacity for oxidative phosphorylation. (3) Loss of diverse mitochondrial functions. Yellow stars represent the electron transport chain, while red stars represent the hydrogenosomal anaerobic ATP generation pathway. Circles represent the mitochondrial genomes.
We have confirmed the presence in A. castellanii of a complete anaerobic ATP generation pathway similar to that found in hydrogenosomes. All of these enzymes are predicted to have mitochondrial localization, and we confirm this localization for the characteristic hydrogenosomal energy enzymes PFO, ASCT1B, HydF and [FeFe]-hydrogenase. It should be noted that we cannot exclude the possibility of a dual mitochondrial and cytosolic localization of these enzymes; nevertheless, the presence in mitochondrial fractions of PFO, ASCT1B and HydF, and the elevated localization of [FeFe]-hydrogenase within the mitochondria, suggests that these organelles can function as a site of anaerobic respiration in A. castellanii. Further research should elucidate the conditions under which this pathway is induced in A. castellanii, and the interplay between anaerobic respiration and encystation as different – or perhaps complementary – survival modes in this organism. In addition, the detection of PFO, ASCT1B and HydF peptides in mitochondrial fractions purified from aerobically grown cells raises the possibility that some or all of these enzymes may be upregulated in response to environmental factors other than oxygen concentration.
The most intriguing question raised by this study concerns the origin of these genes. The physical proximity of [FeFe]-hydrogenase and its three maturases in the genome might suggest the lateral transfer of a single bacterial operon as an acquisition mechanism. However, the presence of so many interspersing genes and the absence of a clear bacterial donor candidate argue against a single, recent transfer from bacteria. Topology tests reject the grouping of A. castellanii and α-proteobacteria (with remaining eukaryotes unconstrained) for [FeFe]-hydrogenase, PFO and ASCT1B. This result is consistent with previous studies, which recover at least two independent origins for [FeFe]-hydrogenase , , , , , and specifically reject α-proteobacterial ancestry for [FeFe]-hydrogenase in topology tests .
Eukaryote monophyly is recovered for two of the maturases and for PFO, and is not rejected in topology tests for [FeFe]-hydrogenase and the remaining maturase. This finding might seem consistent with the hydrogen hypothesis, which holds that the original endosymbiont that gave rise to mitochondrion-related organelles within eukaryotes was a facultative anaerobe possessing an [FeFe]-hydrogenase, retained by a methanogenic, hydrogen-dependent host for the hydrogen it generated as a waste product . Nevertheless, the lack of a clear affinity to α-proteobacterial homologues for these enzymes, and their distribution within eukaryotes – in particular their absence from so many taxa closely related to anaerobes – weakens such a conclusion. All or most of these genes might still have been present in the protomitochondrial endosymbiont as a result of a lateral gene transfer (LGT) event from a different prokaryote, or might have been acquired by the ancestral eukaryote by other means ; these scenarios would be more consistent with the very small number of homologs reported in contemporary α-proteobacteria. However, if this is the case, then (1) the patchy distribution of anaerobic ATP generation enzymes among eukaryotes in general, (2) the rejection of monophyly of A. castellanii with two other amoebozoans for [FeFe]-hydrogenase, and (3) the absence of genes for these enzymes in the genomes of other members of Amoebozoa, remain to be explained.
Intriguingly, the existence of monophyletic eukaryotic clades with unusual internal topology has been reported for other enzymes with patchy distributions among eukaryotes. The authors of these studies proposed multiple lateral transfers between eukaryotes as a hypothesis to explain patchy distributions and unexpected phylogenetic relationships for other enzymes found in both aerobic and anaerobic protists , –. This mode of acquisition would provide an attractive alternative scenario for the acquisition of anaerobic metabolism genes; the transfer of genes between eukaryotes would remove the need for the acquisition of eukaryotic regulatory sequences for the enzymes in the recipient, and would account for the distribution of anaerobic ATP generation enzymes in extant eukaryotes. Furthermore, it would provide an elegant explanation for the common pool of anaerobic ATP generation enzymes found in anaerobic eukaryotes . Frustratingly, the lack of phylogenetic resolution in much of the tree, including for internal eukaryote relationships and for the position of A. castellanii itself, does not allow us to draw strong inferences that would help us to distinguish among competing hypotheses as to the origin of anaerobic enzymes in A. castellanii.
Our work cements the possibility, initially raised by Hug et al. , that anaerobic ATP generation enzymes might be more widespread among eukaryotes than previously thought, not being limited to anaerobic or microaerobic lineages. It also highlights the importance of exploratory sequencing efforts focusing on a wide range of organisms. So far, anaerobic energy enzymes have been described in two non-photosynthetic organisms, A. castellanii and N. gruberi. As an opportunistic human pathogen that also harbours pathogenic bacteria, and a close relative of an opportunistic human pathogen, respectively, both of these organisms have links to human health, making them attractive targets for sequencing efforts. However, the more important link between them is likely their lifestyle, which exposes them to a wide range of habitats that vary in oxygen concentration, a lifestyle likely made possible by the anaerobic energy enzymes they have acquired. Free-living soil protists are relatively poorly studied, and investigations into the metabolic complements of a wider range of such organisms will reveal whether these hybrid organelles are found more commonly in nature than previously suspected.
Methods relating to results shown in Figures S2–S5.
Assessment of mitochondrial purity by comparing rRNA profiles of total cellular RNA and total mitochondrial RNA from A. castellanii. A) An EtBr-stained 6% (w:v) acrylamide, 7 M urea gel loaded with varying quantities of total cellular (T) and total mitochondrial (M) RNA. Numbers are quantities of RNA (in µg) loaded in each lane. The profiles of large rRNA species in T and M are distinct, suggesting that the mitochondrial fraction is relatively free of contaminating cytosolic rRNA (and presumably cytosolic ribosomal proteins). A. castellanii cytosolic (c) and mitochondrial (m) LSU and SSU species are identified (note that the cLSU is split). B) 10% acrylamide gel as in A). Note that a mitochondrial 5S rRNA (m5S) is visualized in the M lane and, to a much lower extent, in the T lane. A band corresponding in size to cytosolic 5.8S rRNA is visible in an overloaded M lane; however, no cytosolic 5S (c5S) is detectable.
Western blot showing recognition of the recombinant [FeFe]-hydrogenase by the homologous anti-[FeFe]-hydrogenase antibody. Lanes 1, 3, 5 and 7: inclusion bodies from C41(DE) cells expressing empty pET-16b vector. Lanes 2, 4, 6 and 8: inclusion bodies from C41(DE) cells expressing recombinant A. castellanii [FeFe]-hydrogenase from pET-16b. Lanes 1 and 2: anti-His-tag antibody, exposed for a shorter period of time than lanes 3–8 in order to avoid overexposure. Lanes 3 and 4: anti [FeFe]-hydrogenase antibody only. Lanes 5 and 6: anti-[FeFe]-hydrogenase antibody incubated with inclusion bodies from C41(DE) cells expression empty pET-16b. Lanes 7 and 8: anti-[FeFe]-hydrogenase antibody incubated with inclusion bodies from C41(DE) cells expressing recombinant A. castellanii [FeFe]-hydrogenase from pET-16b.
Immunogold localization of [FeFe]-hydrogenase in A. castellanii trophozoites exposed to anaerobic conditions for 24 hr. A. Whole cell fixed for immunogold staining; scale bar, 500 nm; this image has been cropped in order to show only the whole cell from which the insets shown were derived. B. and C. Magnified sections from the cell depicted in (A), showing gold particles corresponding to [FeFe]-hydrogenase localization; scale bar, 500 nm. D. Mean density of immunogold labeling in the cytosol, nucleus and mitochondria (8 cells). Brightness and contrast have been adjusted in each image to enhance visibility of the mitochondria and gold particles.
Immunogold localization of [FeFe]-hydrogenase in A. castellanii trophozoites exposed to anaerobic conditions for 6 hr. A. Whole cell fixed for immunogold staining; scale bar, 500 nm; this image has been cropped in order to show only the whole cell from which the insets shown were derived. B. and C. Magnified sections from the cell depicted in (A), showing gold particles corresponding to [FeFe]-hydrogenase localization; scale bar, 500 nm. D. Mean density of immunogold labeling in the cytosol, nucleus and mitochondria (7 cells). Brightness and contrast have been adjusted in each image to enhance visibility of the mitochondria and gold particles.
Phylogeny of [FeFe]-hydrogenase in eukaryotes and bacteria excluding long-branching taxa. Taxa forming a long-branching clade, corresponding to Clade B identified by Hug et al. (2010), have been excluded from these analyses. The topology shown is the ML tree generated by RAxML analyses; 328 sites were examined across 151 taxa. Bootstrap support values ≥50% and posterior probabilities ≥0.5 are shown. Eukaryotes are shaded gray.
Phylogeny of HydE in eukaryotes and bacteria. The topology shown is the ML tree generated by RAxML analyses; 264 sites were examined across 109 taxa. Bootstrap support values ≥50% and posterior probabilities ≥0.5 are shown. Eukaryotes are shaded gray.
Phylogeny of HydF in eukaryotes and bacteria. The topology shown is the ML tree generated by RAxML analyses; 307 sites were examined across 196 taxa. Bootstrap support values ≥50% and posterior probabilities ≥0.5 are shown. Eukaryotes are shaded gray.
Phylogeny of HydG in eukaryotes and bacteria. The topology shown is the ML tree generated by RAxML analyses. 391 sites were examined across 87 taxa; bootstrap support values greater than or equal to 50%, and posterior probabilities greater than or equal to 0.5, are shown. Eukaryotes are shaded gray.
Phylogeny of ASCT1B in eukaryotes and bacteria. The topology shown is the ML tree generated by RAxML analyses; 315 sites were examined across 221 taxa. Bootstrap support values ≥50% and posterior probabilities ≥0.5 are shown. Eukaryotes are shaded gray.
We thank Dr. Brendan Loftus for early sharing of his Acanthamoeba castellanii 454 EST data; Prof. John Walker for providing OverExpress™ C41(DE) cells; Dr. Daniel Gaston for the use of his phylogenetic alignment trimming script; Mary Ann Trevors and Dr. Gary Faulkner for help with immunoelectron microscopy experiments; Dr. Edward Susko for advice on statistical analyses; and Drs. David Spencer, Murray Schnare and Laura Eme for helpful discussion.
Conceived and designed the experiments: MML RMRG MWG AJR. Performed the experiments: MML RMRG. Analyzed the data: MML RMRG MWG. Contributed reagents/materials/analysis tools: MML RMRG MWG AJR. Wrote the paper: MML. Critically read and revised the manuscript: RMRG MWG AJR. Obtained early sequence data: MWG.
- 1. Tsaousis AD, Leger MM, Stairs CAW, Roger AJ (2012) The biochemical adaptations of mitochondrion-related organelles of parasitic and free-living microbial eukaryotes to low oxygen environments. In: Altenbach AV, Bernhard JM, Seckbach J, editors. Anoxia: Evidence for Eukaryote Survival and Paleontological Strategies. Dordrecht: Springer. pp. 51–81.
- 2. Lindmark DG, Muller M (1973) Hydrogenosome, a cytoplasmic organelle of the anaerobic flagellate Tritrichomonas foetus, and its role in pyruvate metabolism. J Biol Chem 248: 7724–7728.
- 3. Dyall SD, Yan W, Delgadillo-Correa MG, Lunceford A, Loo JA, et al. (2004) Non-mitochondrial complex I proteins in a hydrogenosomal oxidoreductase complex. Nature 431: 1103–1107.
- 4. Hrdý I, Hirt RP, Dolezal P, Bardonova L, Foster PG, et al. (2004) Trichomonas hydrogenosomes contain the NADH dehydrogenase module of mitochondrial complex I. Nature 432: 618–622.
- 5. Ellis JE, Williams R, Cole D, Cammack R, Lloyd D (1993) Electron transport components of the parasitic protozoon Giardia lamblia. FEBS Lett 325: 196–200.
- 6. Rodriguez MA, Hidalgo ME, Sanchez T, Orozco E (1996) Cloning and characterization of the Entamoeba histolytica pyruvate: ferredoxin oxidoreductase gene. Mol Biochem Parasitol 78: 273–277.
- 7. Müller M, Mentel M, van Hellemond JJ, Henze K, Woehle C, et al. (2012) Biochemistry and evolution of anaerobic energy metabolism in eukaryotes. Microbiol Mol Biol Rev 76: 444–495.
- 8. Steinbuchel A, Muller M (1986) Anaerobic pyruvate metabolism of Tritrichomonas foetus and Trichomonas vaginalis hydrogenosomes. Mol Biochem Parasitol 20: 57–65.
- 9. Hrdý I, Müller M (1995) Primary structure and eubacterial relationships of the pyruvate:ferredoxin oxidoreductase of the amitochondriate eukaryote Trichomonas vaginalis. J Mol Evol 41: 388–396.
- 10. Ctrnacta V, Ault JG, Stejskal F, Keithly JS (2006) Localization of pyruvate:NADP+ oxidoreductase in sporozoites of Cryptosporidium parvum. J Eukaryot Microbiol 53: 225–231.
- 11. Buetow DE (1989) The mitochondrion. In: Buetow DE, editor. The Biology of Euglena, Vol 4, Subcellular Biochemistry and Molecular-Biology. San Diego: Academic Press. pp. 247–314.
- 12. Gelius-Dietrich G, Henze K (2004) Pyruvate formate lyase (PFL) and PFL activating enzyme in the chytrid fungus Neocallimastix frontalis: a free-radical enzyme system conserved across divergent eukaryotic lineages. J Eukaryot Microbiol 51: 456–463.
- 13. Stairs CW, Roger AJ, Hampl V (2011) Eukaryotic pyruvate formate lyase and its activating enzyme were acquired laterally from a firmicute. Molecular Biology and Evolution 28: 2087–2099.
- 14. Posewitz MC, King PW, Smolinski SL, Zhang L, Seibert M, et al. (2004) Discovery of two novel radical S-adenosylmethionine proteins required for the assembly of an active [Fe] hydrogenase. J Biol Chem 279: 25711–25720.
- 15. Pütz S, Dolezal P, Gelius-Dietrich G, Bohacova L, Tachezy J, et al. (2006) Fe-hydrogenase maturases in the hydrogenosomes of Trichomonas vaginalis. Eukaryot Cell 5: 579–586.
- 16. Hug LA, Stechmann A, Roger AJ (2010) Phylogenetic distributions and histories of proteins involved in anaerobic pyruvate metabolism in eukaryotes. Mol Biol Evol 27: 311–324.
- 17. Tielens AG, van Grinsven KW, Henze K, van Hellemond JJ, Martin W (2010) Acetate formation in the energy metabolism of parasitic helminths and protists. Int J Parasitol 40: 387–397.
- 18. Riviere L, van Weelden SW, Glass P, Vegh P, Coustou V, et al. (2004) Acetyl:succinate CoA-transferase in procyclic Trypanosoma brucei. Gene identification and role in carbohydrate metabolism. J Biol Chem 279: 45337–45346.
- 19. Boxma B, de Graaf RM, van der Staay GW, van Alen TA, Ricard G, et al. (2005) An anaerobic mitochondrion that produces hydrogen. Nature 434: 74–79.
- 20. van Grinsven KW, van Hellemond JJ, Tielens AG (2009) Acetate:succinate CoA-transferase in the anaerobic mitochondria of Fasciola hepatica. Mol Biochem Parasitol 164: 74–79.
- 21. Oulton MM, Amons R, Liang P, MacRae TH (2003) A 49 kDa microtubule cross-linking protein from Artemia franciscana is a coenzyme A-transferase. Eur J Biochem 270: 4962–4972.
- 22. Fritz-Laylin LK, Prochnik SE, Ginger ML, Dacks JB, Carpenter ML, et al. (2010) The genome of Naegleria gruberi illuminates early eukaryotic versatility. Cell 140: 631–642.
- 23. van Grinsven KW, Rosnowsky S, van Weelden SW, Putz S, van der Giezen M, et al. (2008) Acetate:succinate CoA-transferase in the hydrogenosomes of Trichomonas vaginalis: identification and characterization. J Biol Chem 283: 1411–1418.
- 24. Lantsman Y, Tan KS, Morada M, Yarlett N (2008) Biochemical characterization of a mitochondrial-like organelle from Blastocystis sp. subtype 7. Microbiology 154: 2757–2766.
- 25. Stechmann A, Hamblin K, Perez-Brocal V, Gaston D, Richmond GS, et al. (2008) Organelles in Blastocystis that blur the distinction between mitochondria and hydrogenosomes. Curr Biol 18: 580–585.
- 26. Marvin-Sikkema FD, Pedro Gomes TM, Grivet JP, Gottschal JC, Prins RA (1993) Characterization of hydrogenosomes and their role in glucose metabolism of Neocallimastix sp. L2. Arch Microbiol 160: 388–396.
- 27. Gaffron H, Rubin J (1942) Fermentative and photochemical production of hydrogen in algae. J Gen Physiol 26: 219–240.
- 28. Happe T, Kaminski A (2002) Differential regulation of the Fe-hydrogenase during anaerobic adaptation in the green alga Chlamydomonas reinhardtii. Eur J Biochem 269: 1022–1032.
- 29. Forestier M, King P, Zhang L, Posewitz M, Schwarzer S, et al. (2003) Expression of two [Fe]-hydrogenases in Chlamydomonas reinhardtii under anaerobic conditions. Eur J Biochem 270: 2750–2758.
- 30. Horner DS, Heil B, Happe T, Embley TM (2002) Iron hydrogenases–ancient enzymes in modern eukaryotes. Trends Biochem Sci 27: 148–153.
- 31. Hackstein J (2005) Eukaryotic Fe-hydrogenases—old eukaryotic heritage or adaptive acquisitions. Biochem Soc Trans 33: 47–50.
- 32. Meyer J (2007) [FeFe] hydrogenases and their evolution: a genomic perspective. Cell Mol Life Sci 64: 1063–1084.
- 33. Vignais PM, Billoud B (2007) Occurrence, classification, and biological function of hydrogenases: an overview. Chem Rev 107: 4206–4272.
- 34. Horner DS, Hirt RP, Embley TM (1999) A single eubacterial origin of eukaryotic pyruvate: ferredoxin oxidoreductase genes: implications for the evolution of anaerobic eukaryotes. Mol Biol Evol 16: 1280–1291.
- 35. Rotte C, Stejskal F, Zhu G, Keithly J, Martin W (2001) Pyruvate: NADP oxidoreductase from the mitochondrion of Euglena gracilis and from the apicomplexan Cryptosporidium parvum: a biochemical relic linking pyruvate metabolism in mitochondriate and amitochondriate protists. Molecular Biology and Evolution 18: 710.
- 36. Embley TM (2006) Multiple secondary origins of the anaerobic lifestyle in eukaryotes. Philos Trans R Soc Lond B Biol Sci 361: 1055–1067.
- 37. Marciano-Cabral F, Cabral G (2003) Acanthamoeba spp. as agents of disease in humans. Clinical Microbiology Reviews 16: 273–307.
- 38. Cometá I, Schatz S, Trzyna W, Rogerson A (2011) Tolerance of naked amoebae to low oxygen levels with an emphasis on the genus Acanthamoeba. Acta Protozoologica 50: 33–40.
- 39. Turner NA, Biagini GA, Lloyd D (1997) Anaerobiosis-induced differentiation of Acanthamoeba castellanii. Fems Microbiology Letters 157: 149–153.
- 40. Huang X, Madan A (1999) CAP3: A DNA sequence assembly program. Genome Res 9: 868–877.
- 41. Chevreux B, Wetter T, Suhai S (1999) Genome sequence assembly using trace signals and additional sequence information. Computer Science and Biology: Proceedings of the German Conference on Bioinformatics 99: 45–56.
- 42. Chevreux B, Pfisterer T, Drescher B, Driesel AJ, Müller WEG, et al. (2004) Using the miraEST assembler for reliable and automated mRNA transcript assembly and SNP detection in sequenced ESTs. Genome Research 14: 1147–1159.
- 43. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, et al. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25: 3389–3402.
- 44. O'Brien EA, Koski LB, Zhang Y, Yang L, Wang E, et al. (2007) TBestDB: a taxonomically broad database of expressed sequence tags (ESTs). Nucleic Acids Res 35: D445–451.
- 45. Nielsen H, Engelbrecht J, Brunak S, von Heijne G (1997) Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Engineering 10: 1–6.
- 46. Emanuelsson O, Nielsen H, Brunak S, von Heijne G (2000) Predicting subcellular localization of proteins based on their N-terminal amino acid sequence. J Mol Biol 300: 1005–1016.
- 47. Emanuelsson O, Brunak S, von Heijne G, Nielsen H (2007) Locating proteins in the cell using TargetP, SignalP and related tools. Nat Protoc 2: 953–971.
- 48. Lohan AJ, Gray MW (2007) Analysis of 5′- or 3′-Terminal tRNA Editing: Mitochondrial 5′ tRNA Editing in Acanthamoeba castellanii as the Exemplar. Meth Enzymol 424: 221–242.
- 49. Gawryluk RM, Chisholm KA, Pinto DM, Gray MW (2012) Composition of the mitochondrial electron transport chain in Acanthamoeba castellanii: structural and evolutionary insights. Biochim Biophys Acta 1817: 2027–2037.
- 50. Perkins DN, Pappin DJ, Creasy DM, Cottrell JS (1999) Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis 20: 3551–3567.
- 51. Gawryluk RM, Gray MW (2010) Evidence for an early evolutionary emergence of gamma-type carbonic anhydrases as components of mitochondrial respiratory complex I. BMC Evol Biol 10: 176.
- 52. Edgar RC (2004) MUSCLE: a multiple sequence alignment method with reduced time and space complexity. BMC Bioinformatics 5: 113.
- 53. Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32: 1792–1797.
- 54. Bradley RK, Roberts A, Smoot M, Juvekar S, Do J, et al. (2009) Fast statistical alignment. PLoS Comput Biol 5: e1000392.
- 55. Katoh K, Misawa K, Kuma K, Miyata T (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res 30: 3059–3066.
- 56. Katoh K, Kuma K, Toh H, Miyata T (2005) MAFFT version 5: improvement in accuracy of multiple sequence alignment. Nucleic Acids Res 33: 511–518.
- 57. Katoh K, Toh H (2008) Recent developments in the MAFFT multiple sequence alignment program. Brief Bioinform 9: 286–298.
- 58. Criscuolo A, Gribaldo S (2010) BMGE (Block Mapping and Gathering with Entropy): a new software for selection of phylogenetic informative regions from multiple sequence alignments. BMC Evol Biol 10: 210.
- 59. Price MN, Dehal PS, Arkin AP (2009) FastTree: computing large minimum evolution trees with profiles instead of a distance matrix. Mol Biol Evol 26: 1641–1650.
- 60. Stamatakis A (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688–2690.
- 61. Le SQ, Gascuel O (2008) An improved general amino acid replacement matrix. Mol Biol Evol 25: 1307–1320.
- 62. Lartillot N, Lepage T, Blanquart S (2009) PhyloBayes 3: a Bayesian software package for phylogenetic reconstruction and molecular dating. Bioinformatics 25: 2286–2288.
- 63. Le SQ, Lartillot N, Gascuel O (2008) Phylogenetic mixture models for proteins. Philos Trans R Soc Lond B Biol Sci 363: 3965–3976.
- 64. Shimodaira H, Hasegawa M (2001) CONSEL: for assessing the confidence of phylogenetic tree selection. Bioinformatics 17: 1246–1247.
- 65. Gaudet P, Fey P, Basu S, Bushmanova YA, Dodson R, et al. (2011) dictyBase update 2011: web 2.0 functionality and the initial steps towards a genome portal for the Amoebozoa. Nucleic Acids Res 39: D620–624.
- 66. Schneider G, Sjoling S, Wallin E, Wrede P, Glaser E, et al. (1998) Feature-extraction from endopeptidase cleavage sites in mitochondrial targeting peptides. Proteins 30: 49–60.
- 67. Williams K, Lowe PN, Leadlay PF (1987) Purification and characterization of pyruvate: ferredoxin oxidoreductase from the anaerobic protozoon Trichomonas vaginalis. Biochem J 246: 529–536.
- 68. Horner DS, Foster PG, Embley TM (2000) Iron hydrogenases and the evolution of anaerobic eukaryotes. Mol Biol Evol 17: 1695–1709.
- 69. Voncken FG, Boxma B, van Hoek AH, Akhmanova AS, Vogels GD, et al. (2002) A hydrogenosomal [Fe]-hydrogenase from the anaerobic chytrid Neocallimastix sp. L2. Gene 284: 103–112.
- 70. Edwards SW, Lloyd D (1978) Properties of mitochondria isolated from cyanide-sensitive and cyanide-stimulated cultures of Acanthamoeba castellanii. Biochem J 174: 203–211.
- 71. Martin W, Muller M (1998) The hydrogen hypothesis for the first eukaryote. Nature 392: 37–41.
- 72. Andersson JO (2009) Horizontal gene transfer between microbial eukaryotes. Methods Mol Biol 532: 473–487.
- 73. Andersson JO (2009) Gene transfer and diversification of microbial eukaryotes. Annu Rev Microbiol 63: 177–193.
- 74. Andersson JO, Sjogren AM, Horner DS, Murphy CA, Dyal PL, et al. (2007) A genomic survey of the fish parasite Spironucleus salmonicida indicates genomic plasticity among diplomonads and significant lateral gene transfer in eukaryote genome evolution. BMC Genomics 8: 51.
- 75. Takishita K, Chikaraishi Y, Leger MM, Kim E, Yabuki A, et al. (2012) Lateral transfer of tetrahymanol-synthesizing genes has allowed multiple diverse eukaryote lineages to independently adapt to environments without oxygen. Biol Direct 7: 5.
- 76. Andersson JO, Hirt RP, Foster PG, Roger AJ (2006) Evolution of four gene families with patchy phylogenetic distributions: influx of genes into protist genomes. BMC Evol Biol 6: 27.