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National Surveillance Study on Carbapenem Non-Susceptible Klebsiella pneumoniae in Taiwan: The Emergence and Rapid Dissemination of KPC-2 Carbapenemase

  • Sheng-Kang Chiu,

    Affiliations Division of Infectious Diseases and Tropical Medicine, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC, Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan, ROC

  • Tsu-Lan Wu,

    Affiliation Department of Laboratory Medicine, Chang Gung Memorial Hospital, Kweishan, Taoyuan, Taiwan, ROC

  • Yin-Ching Chuang,

    Affiliation Department of Medical Research, Chi Mei Medical Center, Tainan Hsien, Taiwan, ROC

  • Jung-Chung Lin,

    Affiliation Division of Infectious Diseases and Tropical Medicine, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC

  • Chang-Phone Fung,

    Affiliation Section of Infectious Diseases, Department of Medicine, Taipei Veterans General Hospital, National Yan-Ming University, Taipei, Taiwan, ROC

  • Po-Liang Lu,

    Affiliation Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan, ROC

  • Jann-Tay Wang,

    Affiliation Division of Infectious Diseases, Department of Medicine, National Taiwan University Hospital, Taipei, Taiwan, ROC

  • Lih-Shinn Wang,

    Affiliation Department of Infectious Diseases, Buddhist Tzu Chi General Hospital, Hualien, Taiwan, ROC

  • L. Kristopher Siu,

    Affiliation Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, Taiwan, ROC

  • Kuo-Ming Yeh

    Affiliation Division of Infectious Diseases and Tropical Medicine, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC

National Surveillance Study on Carbapenem Non-Susceptible Klebsiella pneumoniae in Taiwan: The Emergence and Rapid Dissemination of KPC-2 Carbapenemase

  • Sheng-Kang Chiu, 
  • Tsu-Lan Wu, 
  • Yin-Ching Chuang, 
  • Jung-Chung Lin, 
  • Chang-Phone Fung, 
  • Po-Liang Lu, 
  • Jann-Tay Wang, 
  • Lih-Shinn Wang, 
  • L. Kristopher Siu, 
  • Kuo-Ming Yeh



The global spread and increasing incidence of carbapenem non-susceptible Klebsiella pneumoniae (CnSKP) has made its treatment difficult, increasing the mortality. To establish nationwide data on CnSKP spread and carbapenem-resistance mechanisms, we conducted a national surveillance study in Taiwanese hospitals.


We collected 100 and 247 CnSKP isolates in 2010 and 2012, respectively. The tests performed included antibiotic susceptibility tests; detection of carbapenemase, extended-spectrum β-lactamases (ESBL), and AmpC β-lactamases genes; outer membrane porin profiles; and genetic relationship with pulsed-field gel electrophoresis and multilocus sequence type.


The resistance rate of CnSKP isolates to cefazolin, cefotaxime, cefoxitin, ceftazidime, and ciprofloxacin was over 90%. Susceptibility rate to tigecycline and colistin in 2010 was 91.0% and 83.0%, respectively; in 2012, it was 91.9% and 87.9%, respectively. In 2010, carbapenemase genes were detected in only 6.0% of isolates (4 blaIMP-8 and 2 blaVIM-1). In 2012, carbapenemase genes were detected in 22.3% of isolates (41 blaKPC-2, 7 blaVIM-1, 6 blaIMP-8, and 1 blaNDM-1). More than 95% of isolates exhibited either OmpK35 or OmpK36 porin loss or both. Impermeability due to porin mutation coupled with AmpC β-lactamases or ESBLs were major carbapenem-resistance mechanisms. Among 41 KPC-2-producing K. pneumoniae isolates, all were ST11 with 1 major pulsotype.


In 2010 and 2012, the major mechanisms of CnSKP in Taiwan were the concomitance of AmpC with OmpK35/36 loss. KPC-2-KP dissemination with the same ST11 were observed in 2012. The emergence and rapid spread of KPC-2-KP is becoming an endemic problem in Taiwan. The identification of NDM-1 K. pneumoniae case is alarming.


Klebsiella pneumoniae is an important pathogen causing various kinds of infection including bacteraemia, pneumonia, liver abscess, and urinary tract infections [1], [2]. The spread of carbapenem non-susceptible Klebsiella pneumoniae (CnSKP) has made its treatment difficult and caused higher disease-related mortality [3], [4]. The incidence of carbapenem resistance among K. pneumoniae in intensive care units in Taiwan increased from 1.2% in 2003 to 11.9% in 2011 [5]. Carbapenem resistance in K. pneumoniae can arise due to the presence of true carbapenemase, or via the combination of impermeability and production of extended-spectrum beta-lactamase (ESBL) or a strongly expressed AmpC enzyme [6], [7]. However, the mechanisms involved in the presence of CnSKP isolates in Taiwan have not been fully investigated.

The carbapenemase enzyme found in K. pneumoniae could be clavulanic-acid-inhibited β-lactamases (Ambler class A families: KPC, NMC, IMI, SME, and GES), metallo-β-lactamases (Ambler class B families: IMP, VIM, NDM-1, GIM, SPM, and SIM), or expanded-spectrum oxacillinases (Ambler class D family: OXA-48) [8]. Carbapenemase-producing K. pneumoniae (KPC-KP) strains were first reported in 2001 from strain isolated in 1996 in the USA [9]. Since then, they have been identified in many places around the world [8], [10][13]. KPC-KP was not reported in Taiwan until 2011 [14], and it could be an emerging threat to clinical practice in Taiwan. Thus, continuous surveillance of antimicrobial resistance is warranted.

CnSKP that exhibits multidrug resistance reduces therapeutic choices and may lead to untreatable infections [8]. Therefore, identification of carbapenemase genes using molecular techniques may help us understand the spread of CnSKP and assist in determining appropriate control measures. In this study, we conducted a nationwide, multicentre prospective surveillance study in Taiwanese hospitals in 2010 and 2012.

Materials and Methods

Hospital Settings

In 2010, 8 medical centres from around Taiwan were enrolled in the study. These included the Keelung Chang Gung Memorial Hospital, Tri-Service General Hospital, Taipei Veterans General Hospital, and Linkou Chang Gung Memorial Hospital in the north; the China Medical University Hospital and Chiayi Chang Gung Memorial Hospital in the central region of the country; and the Kaohsiung Chang Gung Memorial Hospital and Kaohsiung Medical University Hospital in the south. In 2012, the number of participating hospitals was extended to 17, including 9 medical centres and 8 regional hospitals. The hospitals added in 2012 included the National Taiwan University Hospital, Taoyuan Armed Forces General Hospital, Buddhist Tzu Chi General Hospital, Hualien Armed Forces General Hospital, National Yang-Ming University Hospital, Taichung Armed Forces General Hospital, Chi Mei Medical Center, Kaohsiung Armed Forces General Hospital, and Kaohsiung Municipal Hsiaokang Hospital.

Bacterial Isolates

From January through December 2010 and from January through December 2012, CnSKP isolates were collected from clinical specimens sent for culture in the microbiological laboratories of participating hospitals. A single strain was selected per patient. Carbapenem non-susceptibility was defined as a minimum inhibitory concentration (MIC) of at least 2 mg/L for imipenem or meropenem. The isolates collected from each hospital were sent to the National Health Research Institutes, Miaoli, Taiwan and were stored at −70°C in 10% glycerol Luria-Bertani medium before analysis. Species confirmation was performed by standard biochemical methods, on a VITEK 2 automated system (bioMérieux, Marcy l’Etoile, France).

Antimicrobial Susceptibility Testing

MICs for carbapenems (ertapenem, imipenem, meropenem, and doripenem) and other antimicrobial agents [cefazolin, cefotaxime, cefoxitin, cefepime, ciprofloxacin, amikacin, gentamicin, trimethoprim-sulfonamides (SXT), and colistin] were determined by broth microdilution method (Sensititre, Trek Diagnostic Systems, Cleveland, OH, USA). The Clinical and Laboratory Standards Institute (CLSI) M100-S22 interpretive breakpoints were used to interpret the MIC results for all antimicrobial agents studied, except tigecycline and colistin [15]. The MICs for tigecycline were determined using the E-test (AB Biodisk, Solna, Sweden) on Mueller-Hinton media, and susceptibility to tigecycline was defined based on the Food and Drug Administration criteria (MIC ≤2 mg/L) [16]. The susceptibility to colistin was defined based on the European Committee on Antimicrobial Susceptibility Testing criteria (MIC ≤2 mg/L) [17].

Detection of Genes Encoding Carbapenemase, AmpC β-lactamase, and ESBLs

CnSKP isolates were subjected to polymerase chain reaction (PCR) detection of carbapenemase genes (encoding Ambler class A families KPC, NMC, IMI, SME, and GES; Ambler class B families IMP, VIM, NDM, GIM, SPM, and SIM; and Ambler class D family OXA-48-type) [18], plasmid-borne AmpC-like genes (encoding CMY, and DHA) [19], and ESBL genes (encoding CTX-M [20], TEM [20], and SHV [21]). The primers are listed in Table S1. Bacterial DNA was prepared by suspending 1 loop of freshly cultured cells in 500 µL of sterile distilled water and heating the mixture at 95°C for 10 min. The amplification conditions were as follows: 95°C for 5 min, followed by 35 cycles of 95°C for 1 min; 54°C for 1 min; and 72°C for 1 min, with a single, final, elongation step at 72°C for 10 min.

The amplicons were sequenced and the entire sequences were compared were compared to the National Center for Biotechnology Information (NCBI) database at determine the molecular type. Nucleotide sequencing was conducted using corresponding primers specific to blaSHV, blaCTX-M, blaTEM, blaCMY, blaDHA, blaKPC and blaNDM genes with an ABI Prism 3700 DNA sequencer (Applied Biosystems, Foster City, CA, USA).

Identification of Outer Membrane Porins (OmpK35 and OmpK36)

Isolates were grown in high-osmolarity Mueller-Hinton broth to the logarithmic phase and were lysed by sonification. Outer membrane porins (OMPs) were isolated according to the rapid procedure of Carlone et al [22]. The OMP profiles were identified by 12% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by coomassie blue staining (Gibco-BRL, Grand Island, NY, USA). K. pneumoniae ATCC 13883 was used as the control strain.

Pulsed-Field Gel Electrophoresis

Pulsed-field gel electrophoresis (PFGE) was performed for the KPC-KP isolates. In brief, bacterial chromosomal DNAs were digested by using XbaI (New England Biolabs, Beverly, MA, USA) [23]. Electrophoresis was carried out for 22 h at 14°C with pulse times ranging from 2 to 40 s at 6 V/cm with Bio-Rad CHEF MAPPER apparatus (Bio-Rad Laboratories, Richmond, CA, USA). A dendrogram based on the unweighted pair group was generated by the methods previously described [24]. Isolates that had more than 80% similarity on the PFGE profiles were considered as closely related strains.

Multilocus Sequence Type

Multilocus sequence type (MLST) was performed on all isolates from 2010 and KPC-KP in 2012 according to the protocol described on the K. pneumoniae MLST website ( MLST results were typed according to the international K. pneumoniae MLST database created in 2005 at the Pasteur Institute in Paris, France [25].

Statistical Analysis

Statistical analyses were performed using SPSS software package (version 16, Chicago, IL, USA). Analysis was performed by the chi-square test or Fisher’s exact test for categorical variables. A value of p<0.05 was considered statistically significant.


Characteristics of Bacterial Isolates

A total of 100 single-patient isolates were collected in 2010, of which 67 were from northern, 16 were from central, 17 were from southern Taiwan. A total of 247 single-patient isolates were collected in 2012, of which 206 were from northern, 19 were from central, 20 were from southern, and 2 were from eastern Taiwan (Table 1).

Table 1. Regional distributions of the participating hospitals, carbapenem non-susceptible K. pneumoniae isolates, and K. pneumoniae carbapenemase-2-producing K. pneumoniae.

Antimicrobial Susceptibility of CnSKP Isolates

The MIC ranges, MIC50 values, and MIC90 values of 15 antimicrobial agents as well as the rates of susceptibility to said agents against the CnSKP isolates are listed in Table 2. Among the isolates collected in 2010, all isolates (100/100) were resistant to cefazolin, cefoxitin, and ertapenem. Susceptibility rates to cefotaxime and ceftazidime were 1.0%. The susceptibility rates to cefepime, imipenem, and meropenem were less than 10%. Susceptibility rates to gentamicin and amikacin were 11.0% (11/100) and 22.0% (22/100), respectively. The susceptibility rate to ciprofloxacin was 7%. The susceptibility rate to SXT was 10.0% (10/100). Susceptibility rates to tigecycline and colistin were 91.0% (91/100) and 83.0% (83/100), respectively.

Table 2. Results of the antimicrobial susceptibility tests for carbapenem non-susceptible K. pneumoniae isolates collected in 2010 and 2012.

Most of the antimicrobial resistance patterns in isolates collected in 2012 were similar to those observed in isolates collected in 2010. Exceptions to this were the susceptibility rates to gentamicin (44.1% vs. 11.0%, p<0.001), amikacin (66.0% vs. 22.0%, p<0.001), meropenem (26.7% vs. 8.0%, p<0.001), doripenem (25.5% vs. 15.0%, p  = 0.034), and SXT (18.6% vs. 10.0%, p  = 0.048), which were higher than those observed in 2010. The susceptibility rates to tigecycline and colistin in 2012 were 91.9% (227/247) and 87.9% (217/247), respectively.

Susceptibility rates to amikacin (62.6% vs 22.0%, p<0.001), gentamicin (43.9% vs 11.0%, p<0.001), and SXT (20.0% vs 10.0%, p<0.034) were higher in isolates collected in 2012 from original 8 hospitals than isolates collected in 2010. Susceptibility rates to meropenem (11.0% vs 53.5%, p<0.001) and doripenem (8.4% vs 54.3, p<0.001) were lower in isolates collected in 2012 from 8 original hospitals than isolates collected in 2012 from 9 newly participating hospitals.

Detection of Genes Encoding Carbapenemase, AmpC β-lactamase, and ESBLs

Carbapenemase genes were detected in only 6.0% (6/100) of isolates collected in 2010. Four of these isolates expressed blaIMP-8; the other 2 isolates expressed blaVIM-1. All other isolates were negative for all carbapenemases tested. Seventy-eight isolates exhibited Amp-C β-lactamase: 76 isolates had blaDHA-1 and 2 isolates had blaCMY-2. Among the isolates with genes encoding ESBLs, 42 isolates exhibited the CTX-M-9 group (blaCTX-M-14), 4 isolates exhibited the CTX-M-1 group (2 isolates exhibited blaCTX-M-3, 2 isolates exhibited blaCTX-M-15). Twenty-one isolates harboured the SHV-type ESBLs genes (blaSHV-2, blaSHV-2A, blaSHV-5, blaSHV-12, blaSHV-28, and blaSHV-31).

Carbapenemase genes were detected in 22.3% of isolates collected in 2012 (55/247). Further, blaNDM-1 was detected in 1, blaIMP-8 in 6, blaVIM-1 in 7, and blaKPC-2 in 41 isolates. No isolates harboured the blaIMI, blaSME, blaGES, blaNMC and blaOXA-48-type genes. However, 70.8% (175/247) of isolates exhibited Amp-C β-lactamase: 155 and 15 isolates harboured the blaDHA-1 and blaCMY-2 genes, respectively, and 5 isolates carried both blaDHA-1 and blaCMY-2. Among the isolates with genes encoding ESBLs, 157 exhibited the CTX-M-9 group (blaCTX-M-14), 12 exhibited the CTX-M-1 group, and 4 carried both the CTX-M-9 and CTX-M-1 groups. Forty-seven isolates harboured SHV-type ESBLs genes (blaSHV-2, blaSHV-2A, blaSHV-5, blaSHV-12, and blaSHV-120).

Thirty-eight of the 41 KPC-2-KP isolates were from northern Taiwan, 2 were from southern Taiwan, and 1 was from central Taiwan.

Analysis of the OMP Profiles

SDS-PAGE analysis of the isolates from 2010 revealed that 71.0% (71/100) of isolates lacked both OmpK35 and OmpK36 porins, followed by 18.0% (18/100) of isolates without the OmpK35 porin and 9.0% (9/100) of isolates without the OmpK36 porin. Among the isolates collected in 2012, 36.4% (90/247) lacked both the OmpK35 and OmpK36 porins, followed by 55.9% (138/247) of isolates that lacked the OmpK35 porin and 2.4% (6/247) of isolates without the OmpK36 porin. Further, 5.3% (13/247) of isolates did not show outer membrane porin loss. In all, 95.7% of isolates (332/347) exhibited defects either in OmpK35 or in OmpK36. Combination of outer membrane porin profiles with carbapenemase, AmpC β-lactamase, or ESBLs is shown in Table S2.

PFGE Analysis and MLST

PFGE was performed on 100 isolates collected in 2010, and the dendrogram (Figure S1) revealed that there was no dominant clone and inter-hospital spread in 2010. Several intra-hospital clonal spreads were detected by using the 80% similarity as the cut-off.

A dendrogram of the 247 isolates collected in 2012 is shown in Figure S2. The dendrogram and band patterns of KPC-2-producing K. pneumoniae strains are shown in Figure 1. One major pulsotype were identified using the 80% similarity as the cut-off on PFGE profiles. There was a clonal spread of 16 identical isolates obtained from 3 hospitals.

Figure 1. Pulsed-field gel electrophoresis profiles and dendrogram.

Pulsed-field gel electrophoresis profiles and dendrogram of the 41 KPC-2-producing K. pneumoniae isolates. One major pulsotype is shown using the 80% similarity as the cut-off. All these KPC-2-producing K. pneumoniae isolates are ST11.

MLST typing was performed on the 100 isolates collected in 2010 and on the 41 KPC-2-KP isolates in 2012. Among the 2010 isolates, 59.0% (59/100) were sequence type (ST) 11, 6.0% were ST15 (6/100), 7.0% were ST37 (7/100), 5% were ST1 (5/100), 4% were ST524 (4/100), 3% were ST48 (3/100), and 2% were ST147 (2/100). Other ST types had only 1 isolate each. All 41 isolates with the KPC-2 gene in 2012 were ST11.


Until now, no national data specifically on CnSKP in Taiwan has been collected and studied. The present report showed that the major mechanism underlying the CnSKP phenotype in Taiwan was the loss of outer membrane porins combined with β-lactamases such as AmpC enzyme (blaDHA-1 and blaCMY-2) or ESBLs (blaSHV-12, blaCTX-M). A low burden of carbapenemase production among CnSKP was found in Taiwan in 2010, with only 6.0% (6/100) of CnSKP isolates being metallo-β-lactamase (blaIMP-8 and blaVIM-1) producers. In 2010, no other carbapenemase genes such as blaKPC, blaNDM-1, or blaOXA-48-type were detected. However, in 2012, 22.3% (55/247) of CnSKP were carbapenemase producers with blaKPC-2 being detected in 41 isolates (16.6%). NDM-1 CnSKP also appeared in 2012. The clonal spread of ST11 KPC-2-producing K. pneumoniae is occurring with alarming speed, and public-health authorities should be alerted to the potential dissemination of NDM-1 as well.

KPC-KP was not reported in Taiwan until 2011. Chung et al. reported the first case of KPC-2-KP ventilator-associated pneumonia with bacteraemia, in a businessman working in Zhejiang Province, China, when he was transferred to Taiwan for medical management [14]. Lee et al. described an outbreak of 16 KPC-2-KP in 4 hospitals in northern Taiwan between June and September 2011 [26]. Although our study did not include the strains of CnSKP in 2011, the report of Lee et al [26] could be speculated as the origin of ST11 KPC-2-KP in Taiwan. Our study also showed that rapid intra-hospital and inter-hospital dissemination of ST11 KPC-2-KP continued in Taiwan in 2012. The difference between our study and Lee’s study was that we had enrolled different levels of hospitals in northern, central, southern, and eastern Taiwan and we had showed recent molecular basis for the increasing carbapenem resistance among K. pneumoniae. Through this surveillance, we found that ST11 KPC-2-KP had appeared not only in northern Taiwan but also in central and southern Taiwan. During the course of our study, ST11 KPC-2-KP became an endemic problem in Taiwan.

In 2010, the CLSI published new MIC and disk diffusion breakpoints for Enterobacteriaceae [27]. The new MIC breakpoints are 1 to 3 doubling dilutions lower than the original breakpoints (the breakpoint for carbapenems was 4 times lower for imipenem, meropenem, and doripenem and 8 times lower for ertapenem). These changes in the susceptibility breakpoints represent more sensitive criteria for the detection of carbapenem-resistant Enterobacteriaceae and eliminate the need for the detection of carbapenemase activity for making treatment decisions. By using the interpretive criteria with lower MIC breakpoints for carbapenem, the advantage is that laboratories will not miss a carbapenemase producer, while the disadvantage is that organisms having carbapenem resistance under these criteria are probably those with outer membrane porin loss combined with AmpC β-lactamase or ESBL, but not true carbapenemase producers in areas where the burdens of carbapenemase are low. Whether these changes will improve the care outcome of Enterobacteriaceae-infected patients or reduce the potential of carbapenemase needs further evaluation.

According to our results, ST11 was the epidemic clone of the KPC-2-KP in Taiwan in 2012. K. pneumoniae ST11 was first reported in France, and it has been reported around the world, including in the USA, Brazil [28], most countries in Europe [29], and Asia [30]. ST11 is known to be associated with different ESBLs, primarily CTX-M-15, CTX-M-14, and SHV-5 [30], [31]. ST11 is the most predominant type of ESBL-producing K. pneumoniae in Asian countries [32].

There is a single-locus difference between ST11 and ST258; they both belong to the clonal complex CC92 and are considered to be epidemic clones of K. pneumoniae with multi-drug resistance worldwide [33]. ST258 has been reported as the predominant clone of KPC-KP in the USA [34], Israel [35], and Italy [36]. KPC-producing K. pneumoniae ST11 have been reported as the predominant clones of carbapenem-resistant K. pneumoniae in China [37], Singapore [38], and the UK [39]. ST11 was also reported as a common ST for NDM-1-producing K. pneumoniae isolates [40]. ST11 was also reported to be associated with IMP-type metallo-β-lactamase-producing K. pneumoniae in Taiwan between 2002 and 2009 [41]. ST11 may be the major ST among K. pneumoniae isolates in Taiwan. Through the acquisition of different resistance mechanisms, a stepwise increase in resistance to carbapenem and multi-drug regimens causes a high prevalence of ST11 among CnSKP.

Our study on the resistance mechanisms of CnSKP revealed that concomitance of ESBL producers was a general phenomenon. The best choice for treatment should be tigecycline or colistin. Gentamicin, amikacin, cefepime, or SXT is not adequate for treating infections with CnSKP. A resistance rate of 98% for fluoroquinolones has also been reported, and approximately half of KPC-KP isolates were resistant to gentamicin and amikacin, as reported by a study in New York [42].

There were significant differences in the susceptibility rates to meropenem, doripenem, amikacin, gentamicin and SXT between the CnSKP isolates from 2010 and 2012. The true reason for these differences is not currently clear. The possible reasons might be that the newly participating hospitals in 2012 included eight regional hospitals but only one medical center. The antibiotics consumption of meropenem and doripenem might be lower in regional hospitals and might have contributed to the lesser resistance against meropenem and doripenem in 2012 due to lesser antibiotics selective pressure. The antibiotics consumption data needs to be collected in the further study. Another possible reason might be that KPCs are found on plasmids that often carry other β-lactamases and resistance genes [8]. The KPC-2-KP isolates in 2012 should contain plasmids that had different antibiotics resistance genes compared with CnSKP in 2010. The plasmid structure of KPC-2-KP needs further evaluation to answer this question.

Even the current distribution of KPC-2-KP appears to be homogeneous in Taiwan, it is still not clear if the spread of KPC-2 is due to similar or different plasmids. Plasmid related tests (S1-PFGE, restriction fragment length polymorphism ) is helpful to evaluate it. These organisms are commonly isolated from patients in specific regions and hospitals of Taiwan, but they are not regularly found in patients from other regions. Patients colonized or infected with CnSKP may seek medical care in more than 1 hospital, and thereby serve as reservoirs that lead to the spread of CnSKP from 1 facility to another. Based on the survey results, prevention strategies should be tailored to meet the regional needs according to the frequency of CnSKP detection [43].

In conclusion, the major resistance mechanisms of CnSKP in 2010 were due to the production of AmpC-type β-lactamase or ESBLs along with the loss of outer membrane porins, and there were no KPC-producing isolates detected in 2010. In 2012, the emergence of KPC-2 was observed and its incidence increased. More efforts are necessary to prevent the prevalence of CnSKP from increasing. Antibiotic stewardship of carbapenem and infection-control measures should be enforced. National data may help clinicians to choose appropriate antibiotics and to tailor strategies for preventing clonal outbreaks.


The authors would like to thank Dr. Sai-Cheong Lee of KCGMH, Dr. Jen-Hsien Wang of CMUH, Dr. Cong-Yu Huang of CCGMH, Dr. Zhen-Xiang Li of KCGMH, Dr. Ke Chang of KMHH, Dr. Shih-Ta Shang of Taoyuan AFGH, Dr. Zheng-Bang Guo of Taichung AFGH, Dr. Ren-Zhi Ban of KAFGH and Dr. Xin-Bai Chen of NYMUH for their participation in the Study Group of Carbapenem Resistance in Klebsiella pneumoniae in Taiwan.

Supporting Information

Figure S1.

Dendrogram of carbapenem non-susceptible K. pneumoniae isolates in 2010. Dendrogram of XbaI -digested genomic DNA of 100 carbapenem non-susceptible K. pneumoniae isolates collected in 2010. There is no dominated clone causing inter-hospital spread. ST type appears on the right of the figure.



Figure S2.

Dendrogram of carbapenem non-susceptible K. pneumoniae isolates in 2012. Dendrogram of XbaI -digested genomic DNA of 247 carbapenem non-susceptible K. pneumoniae isolates collected in 2012. KPC-2-producing-producing strains are marked with grey colour.



Table S1.

Oligonucleotide primer sequences used for amplification of genes encoding carbapenemase, AmpC β-lactamase, and ESBLs.



Table S2.

Resistance mechanisms among carbapenem non-susceptible K. pneumoniae isolates collected in 2010 and 2012.



Author Contributions

Conceived and designed the experiments: SKC TLW YCC JCL CPF PLL JTW LSW LKS KMY. Performed the experiments: SKC TLW PLL JTW LKS. Analyzed the data: SKC YCC CPF PLL LKS KMY. Wrote the paper: SKC JCL LKS KMY.


  1. 1. Ko WC, Paterson DL, Sagnimeni AJ, Hansen DS, Von Gottberg A, et al. (2002) Community-acquired Klebsiella pneumoniae bacteremia: global differences in clinical patterns. Emerg Infect Dis 8: 160–166.
  2. 2. Siu LK, Yeh KM, Lin JC, Fung CP, Chang FY (2012) Klebsiella pneumoniae liver abscess: a new invasive syndrome. Lancet Infect Dis 12: 881–887.
  3. 3. Patel G, Huprikar S, Factor SH, Jenkins SG, Calfee DP (2008) Outcomes of carbapenem-resistant Klebsiella pneumoniae infection and the impact of antimicrobial and adjunctive therapies. Infect Control Hosp Epidemiol 29: 1099–1106.
  4. 4. Neuner EA, Yeh JY, Hall GS, Sekeres J, Endimiani A, et al. (2011) Treatment and outcomes in carbapenem-resistant Klebsiella pneumoniae bloodstream infections. Diagn Microbiol Infect Dis 69: 357–362.
  5. 5. Annual report of Taiwan Nosocomial Infection Surveillance Systems, 2011. Available: Accessed 2013 Apr 06.
  6. 6. Woodford N, Dallow JW, Hill RL, Palepou MF, Pike R, et al. (2007) Ertapenem resistance among Klebsiella and Enterobacter submitted in the UK to a reference laboratory. Int J Antimicrob Agents 29: 456–459.
  7. 7. Doumith M, Ellington MJ, Livermore DM, Woodford N (2009) Molecular mechanisms disrupting porin expression in ertapenem-resistant Klebsiella and Enterobacter spp. clinical isolates from the UK. J Antimicrob Chemother 63: 659–667.
  8. 8. Nordmann P, Cuzon G, Naas T (2009) The real threat of Klebsiella pneumoniae carbapenemase-producing bacteria. Lancet Infect Dis 9: 228–236.
  9. 9. Yigit H, Queenan AM, Anderson GJ, Domenech-Sanchez A, Biddle JW, et al. (2001) Novel carbapenem-hydrolyzing beta-lactamase, KPC-1, from a carbapenem-resistant strain of Klebsiella pneumoniae. Antimicrob Agents Chemother 45: 1151–1161.
  10. 10. Jean SS, Hsueh PR (2011) High burden of antimicrobial resistance in Asia. Int J Antimicrob Agents 37: 291–295.
  11. 11. Landman D, Bratu S, Kochar S, Panwar M, Trehan M, et al. (2007) Evolution of antimicrobial resistance among Pseudomonas aeruginosa, Acinetobacter baumannii and Klebsiella pneumoniae in Brooklyn, NY. J Antimicrob Chemother 60: 78–82.
  12. 12. Wendt C, Schutt S, Dalpke AH, Konrad M, Mieth M, et al. (2010) First outbreak of Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae in Germany. Eur J Clin Microbiol Infect Dis 29: 563–570.
  13. 13. Landman D, Babu E, Shah N, Kelly P, Olawole O, et al. (2012) Transmission of carbapenem-resistant pathogens in New York City hospitals: progress and frustration. J Antimicrob Chemother 67: 1427–1431.
  14. 14. Chung KP, Tseng SP, Huang YT, Tsai TH, Teng LJ, et al. (2011) Arrival of Klebsiella pneumoniae carbapenemase (KPC)-2 in Taiwan. J Antimicrob Chemother 66: 1182–1184.
  15. 15. Clinical and Laboratory Standards Institute (2012) Performance standards for antimicrobial susceptibility testing: Twenty-Second Informational Supplement M100-S22. Wayne, PA. USA.
  16. 16. Department of Health and Human Services, Food and Drug Administration (2009) Class II special controls guidance document: antimicrobial susceptibility test systems (AST): guidance for industry. Washington, DC. USA. Available: Accessed 2013 Apr 06.
  17. 17. European Committee on Antimicrobial Susceptibility Testing (2013) Breakpoint tables for interpretation of MICs and zone diameters. Version 3.0 ed. Available: Accessed 2013 Apr 06.
  18. 18. Queenan AM, Bush K (2007) Carbapenemases: the versatile beta-lactamases. Clin Microbiol Rev 20: 440–458, table of contents.
  19. 19. Alvarez M, Tran JH, Chow N, Jacoby GA (2004) Epidemiology of conjugative plasmid-mediated AmpC beta-lactamases in the United States. Antimicrob Agents Chemother 48: 533–537.
  20. 20. Eckert C, Gautier V, Saladin-Allard M, Hidri N, Verdet C, et al. (2004) Dissemination of CTX-M-type beta-lactamases among clinical isolates of Enterobacteriaceae in Paris, France. Antimicrob Agents Chemother 48: 1249–1255.
  21. 21. Rasheed JK, Jay C, Metchock B, Berkowitz F, Weigel L, et al. (1997) Evolution of extended-spectrum beta-lactam resistance (SHV-8) in a strain of Escherichia coli during multiple episodes of bacteremia. Antimicrob Agents Chemother 41: 647–653.
  22. 22. Carlone GM, Thomas ML, Rumschlag HS, Sottnek FO (1986) Rapid microprocedure for isolating detergent-insoluble outer membrane proteins from Haemophilus species. J Clin Microbiol 24: 330–332.
  23. 23. D'Agata EM, Gerrits MM, Tang YW, Samore M, Kusters JG (2001) Comparison of pulsed-field gel electrophoresis and amplified fragment-length polymorphism for epidemiological investigations of common nosocomial pathogens. Infect Control Hosp Epidemiol 22: 550–554.
  24. 24. Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, et al. (1995) Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33: 2233–2239.
  25. 25. Diancourt L, Passet V, Verhoef J, Grimont PA, Brisse S (2005) Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates. J Clin Microbiol 43: 4178–4182.
  26. 26. Lee CM, Liao CH, Lee WS, Liu YC, Mu JJ, et al. (2012) Outbreak of Klebsiella pneumoniae carbapenemase-2-producing K. pneumoniae sequence type 11 in Taiwan in 2011. Antimicrob Agents Chemother 56: 5016–5022.
  27. 27. Clinical and Laboratory Standards Institute (2010) Performance standards for antimicrobial susceptibility testing: Twentieth Informational Supplement M100-S20U (June 2010 Update). Wayne, PA, USA.
  28. 28. Andrade LN, Curiao T, Ferreira JC, Longo JM, Climaco EC, et al. (2011) Dissemination of blaKPC-2 by the spread of Klebsiella pneumoniae clonal complex 258 clones (ST258, ST11, ST437) and plasmids (IncFII, IncN, IncL/M) among Enterobacteriaceae species in Brazil. Antimicrob Agents Chemother 55: 3579–3583.
  29. 29. Damjanova I, Toth A, Paszti J, Hajbel-Vekony G, Jakab M, et al. (2008) Expansion and countrywide dissemination of ST11, ST15 and ST147 ciprofloxacin-resistant CTX-M-15-type beta-lactamase-producing Klebsiella pneumoniae epidemic clones in Hungary in 2005–the new 'MRSAs'? J Antimicrob Chemother 62: 978–985.
  30. 30. Ko KS, Lee JY, Baek JY, Suh JY, Lee MY, et al. (2010) Predominance of an ST11 extended-spectrum beta-lactamase-producing Klebsiella pneumoniae clone causing bacteraemia and urinary tract infections in Korea. J Med Microbiol 59: 822–828.
  31. 31. Hrabak J, Empel J, Bergerova T, Fajfrlik K, Urbaskova P, et al. (2009) International clones of Klebsiella pneumoniae and Escherichia coli with extended-spectrum beta-lactamases in a Czech hospital. J Clin Microbiol 47: 3353–3357.
  32. 32. Lee MY, Ko KS, Kang CI, Chung DR, Peck KR, et al. (2011) High prevalence of CTX-M-15-producing Klebsiella pneumoniae isolates in Asian countries: diverse clones and clonal dissemination. Int J Antimicrob Agents 38: 160–163.
  33. 33. Pereira PS, de Araujo CF, Seki LM, Zahner V, Carvalho-Assef AP, et al.. (2012) Update of the molecular epidemiology of KPC-2-producing Klebsiella pneumoniae in Brazil: spread of clonal complex 11 (ST11, ST437 and ST340). J Antimicrob Chemother.
  34. 34. Kitchel B, Rasheed JK, Patel JB, Srinivasan A, Navon-Venezia S, et al. (2009) Molecular epidemiology of KPC-producing Klebsiella pneumoniae isolates in the United States: clonal expansion of multilocus sequence type 258. Antimicrob Agents Chemother 53: 3365–3370.
  35. 35. Kallen A, Guh A (2012) United States Centers for Disease Control and Prevention issue updated guidance for tackling carbapenem-resistant enterobacteriaceae. Euro Surveill 17.
  36. 36. Mammina C, Palma DM, Bonura C, Anna Plano MR, Monastero R, et al. (2010) Outbreak of infection with Klebsiella pneumoniae sequence type 258 producing Klebsiella pneumoniae Carbapenemase 3 in an intensive care unit in Italy. J Clin Microbiol 48: 1506–1507.
  37. 37. Qi Y, Wei Z, Ji S, Du X, Shen P, et al. (2011) ST11, the dominant clone of KPC-producing Klebsiella pneumoniae in China. J Antimicrob Chemother 66: 307–312.
  38. 38. Balm MN, Ngan G, Jureen R, Lin RT, Teo J (2012) Molecular characterization of newly emerged blaKPC-2-producing Klebsiella pneumoniae in Singapore. J Clin Microbiol 50: 475–476.
  39. 39. Virgincar N, Iyer S, Stacey A, Maharjan S, Pike R, et al. (2011) Klebsiella pneumoniae producing KPC carbapenemase in a district general hospital in the UK. J Hosp Infect 78: 293–296.
  40. 40. Giske CG, Froding I, Hasan CM, Turlej-Rogacka A, Toleman M, et al. (2012) Diverse sequence types of Klebsiella pneumoniae contribute to the dissemination of blaNDM-1 in India, Sweden, and the United Kingdom. Antimicrob Agents Chemother 56: 2735–2738.
  41. 41. Ma L, Lu PL, Siu LK, Hsieh MH (2013) Molecular typing and resistance mechanisms of imipenem-non-susceptible Klebsiella pneumoniae in Taiwan: results from the Taiwan surveillance of antibiotic resistance (TSAR) study, 2002–2009. J Med Microbiol 62: 101–107.
  42. 42. Bratu S, Tolaney P, Karumudi U, Quale J, Mooty M, et al. (2005) Carbapenemase-producing Klebsiella pneumoniae in Brooklyn, NY: molecular epidemiology and in vitro activity of polymyxin B and other agents. J Antimicrob Chemother 56: 128–132.
  43. 43. Centers for Disease Control and Prevention (2012) Guidance for Control of Carbapenem-resistant Enterobacteriaceae. Available: h. Accessed 2013 Apr 06.