Stable carbon isotope analysis in tooth enamel is a well-established approach to infer C3 and C4 dietary composition in fossil mammals. The bulk of past work has been conducted on large herbivorous mammals. One important finding is that their dietary habits of fossil large mammals track the late Miocene ecological shift from C3 forest and woodland to C4 savannah. However, few studies on carbon isotopes of fossil small mammals exist due to limitations imposed by the size of rodent teeth, and the isotopic ecological and dietary behaviors of small mammals to climate change remain unknown. Here we evaluate the impact of ecological change on small mammals by fine-scale comparisons of carbon isotope ratios (δ13C) with dental morphology of murine rodents, spanning 13.8 to ∼2.0 Ma, across the C3 to C4 vegetation shift in the Miocene Siwalik sequence of Pakistan. We applied in-situ laser ablation GC-IRMS to lower first molars and measured two grazing indices on upper first molars. Murine rodents yield a distinct, but related, record of past ecological conditions from large herbivorous mammals, reflecting available foods in their much smaller home ranges. In general, larger murine species show more positive δ13C values and have higher grazing indices than smaller species inhabiting the same area at any given age. Two clades of murine rodents experienced different rates of morphological change. In the faster-evolving clade, the timing and trend of morphological innovations are closely tied to consumption of C4 diet during the vegetation shift. This study provides quantitative evidence of linkages among diet, niche partitioning, and dental morphology at a more detailed level than previously possible.
Citation: Kimura Y, Jacobs LL, Cerling TE, Uno KT, Ferguson KM, Flynn LJ, et al. (2013) Fossil Mice and Rats Show Isotopic Evidence of Niche Partitioning and Change in Dental Ecomorphology Related to Dietary Shift in Late Miocene of Pakistan. PLoS ONE 8(8): e69308. doi:10.1371/journal.pone.0069308
Editor: Laurent Viriot, Team ‘Evo-Devo of Vertebrate Dentition’, France
Received: February 22, 2013; Accepted: June 6, 2013; Published: August 2, 2013
Copyright: © 2013 Kimura et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by the Society of Vertebrate Paleontology (Mary R. Dawson Grant to YK), the Geological Society of America (Graduate Student Research Grant to YK), and ISEM (travel grants to YK) at Southern Methodist University. Other financial support was provided by NSF EAR-0959093 to TEC, NSF EAR-0958178 to LJF, and PURSE grant to RP. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Stable carbon isotope ratios (δ13C) in bioapatite reflect carbon isotope compositions of diets through physiological processes in animals ,  and have been widely used to reconstruct dietary preferences between C3 and C4 plants, resource partitioning among coexisting species, and vegetation types in their habitats (,  and references therein). Stable carbon isotope analysis along with tooth crown height can characterize feeding strategies of fossil mammals , , . Grimes et al.  addressed advantages of using small mammals (e.g., rodents) over large mammals for isotopic paleoenvironmental reconstruction. Higher temporal and spatial resolution would be possible owing to a more abundant fossil record. Stable isotope ratios in small mammals would reflect more local vegetation and environment in their habitats because of their small home ranges without seasonal migration. However, stable isotope studies of small mammals have lagged behind those of large herbivores because obtaining an adequate amount of CO2 sample from bioapatite in tiny teeth of small mammals is more difficult. Thus, few studies have been conducted using carbon isotope analysis of rodent teeth. In the previous studies, incisor enamel was preferably used , , ,  unless molars were large enough to provide the necessary yield of CO2 sample from a single individual , ,  or molar enamel derived from multiple individuals was combined . Fossil rodents are usually preserved as isolated teeth in sedimentary deposits and are identified almost exclusively based on molar morphology. Isolated incisors themselves can only be classified more-or-less at the order or family level. In using rodent incisors, paleoecological studies of rodents can be done only in those special cases in which incisors are identifiable, for instance, because they are recovered with complete skulls and jaws from a fossil locality with a limited diversity of rodents.
The in-situ infrared laser ablation method improved by Passey and Cerling  makes it possible to measure δ13C in teeth with 10 to 30 nmol of CO2 sample , meaning that rodent molars, which are ∼1.5 mm or larger, can generate a single data point from a single specimen using this method. Importantly, post-ablation teeth retain dental morphology for paleontological studies. Hynek et al.  applied the laser ablation method to incisor enamel and concluded that rodents are sensitive recorders of paleovegetation by comparing temporal change of δ13C in rodents with those of notoungulates and soil carbonates. Gehler et al.  suggested that carbon isotope compositions in incisor enamel record dietary differences between modern murine and arvicoline rodents. Therefore, carbon isotope analysis of rodent molars has potential to reveal past ecosystems at more detailed levels than has been achieved with rodent incisors or with large mammals.
Here, we show the significance of using molars of fossil rodents in geochemical analysis along with paleontological approaches to understand interactions between ecology and evolutionary biology of animals by comparing δ13C data in molar enamel of murine rodents with morphometric distances in molars that are ecomorphological characters for grazing on finest scale yet achieved through the Miocene Siwalik sequence, Pakistan. The ecological shift from C3 trees and shrubs to vegetation dominated by C4 grasses was first recognized in Siwalik Group rocks in Pakistan through a temporal sequence of carbon isotopes in soil carbonates . This global event is observed between 8.5 and 6.0 million years (Ma) ago at low to mid-latitudes , , , . The vegetation shift influenced dietary habits of large herbivorous mammals by changing the proportion of plant species available for food ( and references therein). Murine rodents from the Siwalik Group were chosen for this study because well-documented δ13C data of large herbivorous mammals and soil carbonates from the region are available for comparisons, making the Siwaliks an ideal experimental test, and no other small mammal taxa from the region approach the abundance of murine rodents  during the time interval surrounding the ecological and vegetation shift.
Murine rodents (Old World rats and mice) are the most diverse and abundant of modern mammal subfamilies, comprising over 550 species . Siwalik murine fossils represent the best and longest record of murine evolution from the earliest occurrence of the unambiguous murine rodent, Antemus, at 13.8 Ma . Their relative abundance increased at an accelerated rate since the first appearance of Antemus to become dominant over cricetid rodents at about 11 Ma (YGSP 76), when Progonomys appeared . Jacobs  and Jacobs and Downs  recognized gradual changes of dental morphology through time, which resulted primarily from in-situ evolution in northern Pakistan. They proposed two fundamental lineages derived from Antemus: the Progonomys clade containing Progonomys and Mus (mice), and the Karnimata clade containing Karnimata, Parapelomys, and potentially Rattus (rats). The dichotomous lineages are a simplified evolutionary hypothesis but capture overall morphological trends of Siwalik murine rodents , , . Thus, the effect of ecological change on the evolution of small mammals can be most effectively tested by utilizing the finely-spaced fossil record of Siwalik murine rodents with minimum influence of immigration from other regions.
Materials and Methods
We measured δ13C values in lower first molars (m1) of murine fossils collected from the Siwalik Group in the Potwar Plateau of northern Pakistan, ranging from13.8 to 6.5 Ma (21 localities), and northern India, ranging from ∼2.5 to ∼1.8 Ma (2 localities), and compared them with the compiled data of large herbivorous mammals and soil carbonates presented in Figure 2 of Badgley et al. . Carbon isotopes in enamel reflect dietary input during the time of tooth formation, whereas dental morphological characters are long-term adaptive traits obtained through evolutionary history. Two ecomorphological characters, van Dam's  index (VD index hereafter, defined below) and tooth crown height (hypsodonty) were evaluated to compare timing and direction of morphological change with isotopic dietary inferences.
To minimize inter-tooth isotopic variation, we deliberately analyzed only m1 (except several samples from the Pliocene of India). Isotope compositions across teeth in different tooth positions are influenced by nursing to different degrees (Figure S1) because mineralization of molar enamel starts before weaning in murine rodents (see Supporting Information S1). Although we do not exactly know the carbon isotope effect of nursing on murine molars, the magnitude of relative change across the data of m1 should reflect actual changes in the Siwalik murines, assuming that physiological differences in the closely-related species are negligible. We chose m1 over the second and third molars even though m1 is most influenced by mother's milk because m1 is large enough (1.5 to 2.9 mm in length) to generate a CO2 sample from a single tooth, and morphological complexity of m1 is useful for systematic identification and allows for species-level comparisons.
Samples ranging in age from 13.8 to 6.5 Ma were collected by sieving in the 1970's to 2000 from the Potwar Plateau, northern Pakistan. The fossil specimens are on long-term loan from Pakistan, under the authority of the Geological Survey of Pakistan, Islamabad, Pakistan, and are housed in the Peabody Museum of Archaeology and Ethnology, Harvard University. To fill the time gap between 6.5 Ma and Recent, five specimens from Kanthro (∼2.5 Ma) and six specimens from Nadah (∼2.0 to ∼1.8 Ma) recovered from Upper Siwalik localities of northern India ,  were analyzed. All Indian specimens used in this study are housed in the Centre of Advanced Study in Geology, Panjab University. Recent specimens from both regions were also utilized for comparison. Among those from Pakistan, Golunda ellioti and two Mus species (M. booduga, M. saxicola) were from owl pellets collected on the Potwar Pleatau, whereas Rattus sp. and Millardia sp. were captured in urban areas of the Potwar between 1970 and 1975. They are housed in the Shuler Museum of Paleontology, Southern Methodist University. Bandicota indica was collected from an owl pellet in Nagar, Punjab State, India in 2011. Specimen numbers are given in Datasets S1 and S2. No additional permits were required for the described study, which complied with all relevant regulations.
Specimen Identification and Sample Selection
Jacobs ,  described rodent specimens from localities YGSP 491 (13.8 Ma), YGSP 41 and 430 (13.6 Ma), YGSP 182 (9.2 Ma), DP 13 (6.5 Ma), and DP 24 (∼1.7 Ma), and recognized 9 species among 8 genera. Cheema et al.  described Progonomys hussaini from locality JAL-101 (∼11 Ma). Specimens from other localities have been identified at the generic level or, if not, were assigned to ambiguous groups such as “near Antemus” according to the most updated study by Jacobs and Flynn . The qualitative nature of the taxonomic assignments and anagenetic change in time-consecutive taxa impede the systematic classification of the Siwalik murines. We grouped tooth samples at each stratigraphic level based on size and morphology, so that individuals in the same assemblage display high similarity and generally followed Jacobs and Flynn  for names of taxa (Table S1). We analyzed all murine species known with n>5 by upper first molar (M1) at each stratigraphic level, as well as large Karnimata sp., which are minor components (n<5 by M1) at 8.2 and 8.8 Ma (Table S1). Complete m1 with slight to moderate wear, corresponding to wear stage I to IV of Lazzari et al. , were selected for carbon isotope analysis. The tooth samples are neither weathered nor etched. The enamel is intact based on visual observation under a light microscope with 100× magnification for all and SEM observation for randomly selected specimens. Diagenetic alteration was assumed to be negligible for the murine rodent teeth as confirmed by Quade et al.  for large mammal teeth from the Siwalik sediments of similar ages in the same region.
Carbon Isotope Analysis
We conducted in-situ laser ablation GC-IRMS isotope analyses on enamel of m1 using the laser ablation method described by Passey and Cerling  at the University of Utah. Isotope ratios are expressed as δ values (‰) on VPDB scale, where δsample = (Rsample/Rstandard-1)*103, and R is given by 13C/12C. Fossil specimens were cleaned by acetone to remove glue and were treated with 2% NaOCl for one hour to remove organic contaminants, followed by 0.1 M sodium acetate-acetic acid buffer for one hour (except specimens from YGSP 182, see below) to remove diagenetic carbonate minerals from enamel surfaces. The time period for the 2% NaOCl treatment varied from 3 hours to 8 hours in modern specimens, depending on the amount of organic tissue adhered to the specimen. The 0.1 M acetic acid buffer (pH = ∼5.4) was used rather than 0.1 M acetic acid (pH = ∼3.0) because thin enamel of murine molars are etched by 0.1 M acetic acid in only 15 minutes as observed under SEM (Figure S2). The treated samples were mounted on a plate of the laser sample chamber using Bostik Blu-Tack. The lingual side was preferentially used, but the labial and posterior sides were also analyzed when data results from the lingual side were not reliable due to low CO2 yield or contamination of CO2 generated from ablation of dentine or Blu-Tack. CO2 laser (wave length: 10.6 μm) setting ranged from 1.8 to 7.5 W with a 8.5 ms pulse duration. CO2 from multiple ablation pits was cryogenically concentrated, inlet to a gas chromatographic column (Poraplot Q; 60°C) and sent into a Finnigan MAT 252 gas-ratio mass spectrometer via a GC/CP interface.
Isotope data reported in this study were corrected for isotope fractionation between laser and conventional H3PO4 methods to be comparable with the compiled data of Badgley et al. , in which carbon isotope values were obtained by the H3PO4 method. Isotope enrichment (ε) between two substances A and B is defined as εA−B = (RA/RB −1)*1000 , , which is independent on the scale (i.e., PDB, SMOW) and more preferable than the scale-specific difference of ΔA−B = δA–δB , . The superscript * (ε*) is designated for non-equilibrium processes . The mean 13ε*laser-H3PO4 value was −1.5±0.3‰ (1σ) for a modern beaver incisor analyzed in every run, whose isotope values had been determined by the H3PO4 method. This value is larger than the value (13ε*laser-H3PO4 = −0.3‰) reported by Passey and Cerling  but is smaller than those of Podlesak et al. . The mean 18ε*laser-H3PO4 value, −6.1±0.9‰, was a much larger offset than that of 13ε*laser-H3PO4 values. This is because laser ablation of enamel liberates both phosphate and carbonate bound oxygen, whereas the H3PO4 method targets only carbonate oxygen. There is a ∼9 permil offset between phosphate and carbonate bound oxygen . Oxygen isotope data produced by the CO2 laser ablation method are less precise than isotopic measurements of phosphate oxygen or structural carbonate oxygen due to incomplete mixing of oxygen-bearing components . We do not discuss oxygen isotope compositions of Siwalik murine rodents in this study. Carbon isotope compositions of Recent species reflect light carbon inputs to the atmosphere by burning fossil fuels. They were corrected for δ13C values (−6.5‰) of atmospheric CO2 in pre-industrial times . Modern δ13C atmosphere in years of sampling (1970's and 2011) are −7.4‰  and −8.2‰ , respectively.
Nine specimens from YGSP 182 (Dataset S1) were treated with 0.1 M acetic acid for 8 hours and resulted in strongly etched enamel surface. These initial treatment results led us to check the effect of the acetic acid treatment on murine enamel. By observation under SEM, removal of non-prismatic outer enamel started in only 15 minutes in a 0.1 M solution due to the low pH of the acetic acid (pH = ∼3.0) (Figure S2). Thus, the 0.1 M acetic acid treatment was replaced with 0.1 M sodium acetate-acetic acid buffer (pH = ∼5.4). The isotopic composition of the YGSP 182 specimens may have shifted by the acetic acid treatment. In Koch et al. , powdered enamel samples treated with 0.1 M acetic acid for three days were altered by −0.2 ‰ for δ13C and by +0.9 ‰ for δ18O compared to untreated samples. In the YGSP 182 specimens, the mean isotope ratio of the etched samples (n = 9) are offset by −1.1‰ in carbon and by +1.5‰ in oxygen, compared to samples (n = 2) treated by buffered acetic acid. Although the direction of the acid effect is the same as demonstrated in Koch et al. , the absolute values for correction are not known in this study. The nine specimens are not corrected for the difference in the acid treatment.
Quantifying Dental Characters
The VD index evaluates space between anteroposteriorly aligned cusps on upper first molar (M1), defined as the ratio of tooth width to a distance between the posterior side of the lingual anterocone and that of protocone. Species with a value of more than 2.2 are predominantly grazers , . Note that van Dam  used two other characters along with VD index in a principle component analysis in order to distinguish grazing murines from non-grazers. Both characters are based on m2, which are more difficult to identify and are not used in this study. Following the result of van Dam , taking 2.2 as a critical value for grass diets is reasonable although it does not necessarily mean that every species with >2.2 is a grazer.
Hypsodonty is well-studied in ungulates and is associated with adaptation to high rates of tooth wear due to tough diets together with ingestion of soil and grit while feeding , . Hypsodonty index is most commonly determined by height/width of unworn m3 . In this study, hypsodonty was measured as crown height divided by length of M1. The length was used instead of width because its variance was less than half that of width among the species identified by Jacobs , .
Specimens used for VD index are unworn to moderately worn, corresponding to wear stage I to IV of Lazzari et al. , and those used for hypsodonty are unworn or slightly worn specimens in wear stage I to II. All dental measurements were taken in VHX-1000 communication software based on 2D digital pictures photographed with a Keyence VHX-1000 digital microscope. In a mean of VD index for each species, large Karnimata sp. at 9.2 Ma (YGSP 7717) was included in K. darwini due to small sample size.
The rates of temporal change in δ13C values were compared between two anagenetic lineages, Karnimata of the Karnimata clade (blue circles in figures) and the Progonomys clade without Mus sp. at 7.4 Ma (red triangles in figures), from 9.2 through 6.5 Ma by using linear regression lines between δ13C values (dependent variable) and time (independent variable, covariate) of the two clades (categorical variable). That species of Mus sp. was excluded because it may be an immigrant from another region, based on its smaller size and more elongated anterior side of the lingual and labial anterocones than those of the younger Mus auctor (6.5 Ma). To avoid confusion, the Progonomys clade without Mus sp. at 7.4 Ma is expressed as the “Progonomys clade” hereafter. We tested the interaction between the categorical variable and the covariate in the aov() command of R for a null hypothesis that they do not interact. Because the null hypothesis was accepted, i.e., slopes of the two regression lines are equal (p = 0.54), Analysis of covariance (ANCOVA) was performed to test if intercepts of the linear regression lines between the two clades are significant. Before performing the linear regression, we checked that a linear model fitted the data as well as or better than a quadratic model. Temporal changes in VD index and hypsodonty values between the two clades were also examined in the same procedure. We did not adopt linear regression analysis between the ecomorphological characters and δ13C values (e.g., VD index vs. mean δ13C values) because the variables are at different time scales. Carbon isotope compositions in enamel are a short-term variable, reflecting mixing of two end-members, C3 plants and C4 plants, in their diets during enamel mineralization. On the other hand, VD index and hypsodonty are long-term variables that species acquired along phylogeny. As a result, linear regressions between the ecomorphological characters and δ13C values have lower R2 values than linear regressions of temporal changes in the three variables.
At 7.4 Ma, 6.5 Ma, and Recent, in which three species were considered, Welch's ANOVA was performed to assess whether means of δ13C values in the three coexisting species are indistinguishable at α = 0.05. Welch's ANOVA was chosen to avoid increasing Type II error rates due to small sample sizes. The Games-Howell test was used for post-hoc tests to identify the location of significant differences at α = 0.05.The assumptions of normality and homogeneity of variance were checked by the Shapiro-Wilk test and the Bartlett's test. Recent Millardia sp. was combined with Recent Rattus sp. due to small sample size. Although molecular studies show they are not very closely related , Millardia was sometimes considered a subgenus of Rattus . Recent Mus spp. includes Mus booduga and Mus saxicola. The 95% bootstrap confidence intervals were computed by the bias-corrected and accelerated method with 9999 randomizations in the ‘boot’ package . All statistical tests but Welch's ANOVA and Games-Howell test, which were computed in IBM SPSS Statistics 18 , were performed in R2.15.1 . Samples whose values clearly deviate from others in the same species and are beyond three standard deviations were removed from statistical tests as outliers.
Stable carbon isotope data combined for all murine species are shown in Figure 1, and summarized in Table 1 and Table S2 (Dataset S1). A total of 189 carbon isotope data points were obtained from modern and fossil murines from the Siwalik Group. Each data point is represented by a single molar.
(B) Carbon isotope ratios of enamel in the lower first molars (m1) of Siwalik fossil and Recent murines. Ages of the Siwalik localities derived from paleomagnetic stratigraphy are based on Geomagnetic Polarity Time Scale of Ogg and Smith .
The δ13C values became abruptly more positive with a broader range at 7.4 Ma. The mean δ13C value is −10.8‰ for pre-7.4 Ma (i.e., 13.8 to 7.8 Ma) and becomes −6.6‰ at 7.4 Ma. The range value of 9.0‰ at 7.4 Ma is a threefold increase compared to previous age intervals. Predating the rapid increase, there is a gradual negative shift of 2.5‰ between 13.8 Ma and 8.7 Ma. A relatively large offset at 9.2 Ma compared to 9.0 Ma may be partly attributed to the difference in the acid procedure (see Carbon Isotope Analysis in Material and Methods). From 8.7 to 8.2 Ma, the mean δ13C value shifted by +1.5‰ without changing the range of variation. Despite a slight increase from 8.7 to 8.2 Ma, the maximum values at 8.2 Ma and 8.0 Ma do not exceed the maximum value at 13.8 Ma.
The rates of temporal change in δ13C values are not significantly different (p = 0.54, Figure S3, Table S3) between Karnimata (large species, blue circles in figures) and the “Progonomys clade” (small species, red triangles in figures), indicating both clades experienced the same degree of dietary change from 9.2 through 6.5 Ma. ANCOVA shows intercepts of the regression lines are significantly different between the clades (p = 0.002, Table S3), meaning that Karnimata species have a greater mean δ13C value than the “Progonomys clade” when the effect of time is controlled. Each linear model predicts 73% and 69% of the variance in δ13C values by time, respectively (p<0.001, Table S4). Based on the linear regression lines, Karnimata species and the “Progonomys clade” increase δ13C values by 3.0‰ and 2.8‰ per million years, respectively.
The VD index of Karnimata is positively related to time (R2 = 0.56, p<0.001), whereas there is no significant linear relationships between VD index and time in the “Progonomys clade” (Table S4, Dataset S2). The rates of temporal change in VD index are significantly different (p<0.001, Figure S4, Table S3) between the clades, indicating that Karnimata species increase VD index values through time, while VD index is constant in the “Progonomys clade”. Hypsodonty shows no relationship with time in either clade (Table S4, Dataset S2), but ANCOVA shows the observed difference in the intercepts is marginally significant (p = 0.04, Figure S5, Table S3), meaning that Karnimata species have higher tooth crowns than the “Progonomys clade”.
Statistical tests showed significant differences in mean δ13C values among coexisting species at 7.4 Ma, 6.5 Ma, and Recent (Tables S5 and S6). For 7.4 Ma and 6.5 Ma, post-hoc tests show a mean of one species is significantly different from those of other coexisting species. At 7.4 Ma, the smallest species, Mus sp. (green diamonds in figures), has a mean δ13C value more negative than Progonomys sp. and Karnimata sp. by 2.6‰ and 3.5‰, respectively. At 6.5 Ma, a mean δ13C value of the largest species, Parapelomys robertsi (light blue squares in figures), is more negative than that of Mus auctor and Karnimata huxleyi by 2.4‰ and 4.3‰, respectively.
Comparisons with Carbon Isotope Ratios in Large Mammals and Soil Carbonates
An abrupt positive shift of δ13C values indicates a pronounced dietary shift of murine rodents between 7.8 and 7.4 Ma. The dietary shift is more abrupt in murine rodents (Figure 1) than in large mammals . Comparisons at different systematic levels may contribute to the fact that large mammals have wider variation of δ13C values than murine rodents. However, the ranges of δ13C values in murine rodents are concordant with variation within 5 to 95 percentiles of δ13C values in large mammals, ∼3‰ for 13 to 11 Ma and ∼9‰ for 7.4 to 7.0 Ma. Hipparionine equids incorporated C4 grasses into their diet by ∼8.5 Ma , , whereas murine rodents lagged behind these ungulates by one million years. The pattern of δ13C values in murine rodents is similar to the isotopic shift recorded in soil carbonates, which reflect the overlying vegetation more directly than large mammal data. It suggests that murine rodents record past ecological conditions more precisely than large mammals because of their feeding behavior as dietary opportunists with small home ranges in short life span.
In contrast, neither large mammals nor soil carbonates record the gradual negative shift of 2.5‰ toward 8.7 Ma observed in murine data. The negative shift may reflect an actual change in vegetation because the most negative mean δ13C values in large mammals and soil carbonates are at 9.2 to 8.6 Ma and at 9.2 to 8.3 Ma, respectively, which encompasses the 8.7 Ma level of the murine data.
Dietary Niche Partitioning
From 9.2 Ma through 6.5 Ma, large murine species have a consistently greater mean δ13C value than small species with the exception of Parapelomys robertsi (Figure 2). Carbon isotope ratios in coexisting species can vary due to different patterns in spatial occupation. In a modern ecosystem, Codron et al.  showed δ13C values of C3 plants vary by up to 2‰ in an African savanna, having more negative values associated with availability of perennial water sources. Thus, the isotopic variation (less than 1.7‰) between large and small murine species observed from 9.2 Ma through 8.2 Ma may be explained by spatial partitioning, with small species feeding on less water-stressed C3 plants. Considering isotopic enrichment of insects and non-photosynthetic plant tissues relative to green leaves , , the magnitude of the isotopic variation can also be explained by dietary niche partitioning, with large species feeding on more strictly vegetarian diets. The intake of C4 grasses might be partially responsible for the isotopic differentiation as early as 8.2 Ma because isotopes in hipparionine equids indicate a C4 dietary component beginning at 8.5 Ma in Pakistan . Nevertheless, C3 vegetation still dominated major floodplains at ∼8.0 Ma .
Outliers removed from statistical tests are shown by asterisks.
The isotopic variation of less than 2‰ may also result from proportional contributions of insects and non-photosynthetic tissues of C3 plants in the diet of murine rodents. Most of today's murine rodents are omnivorous , consuming a variety of plant material (seeds, grains, nuts, fruits, leaves, stems, roots), insects, and other invertebrates. Insects tend to be more enriched in 13C than primary producers due to trophic enrichment . The mean estimate of trophic shift is +0.6‰ among terrestrial arthropods, which are one of major food source for murines, based on data compiled in McCutchan et al. . The isotopic differentiation resulting from different proportional amounts of insects is observed in modern Rattus rattus and Mus musculus inhabiting forests of Hawaii, where they partition food resources in that Rattus rattus is primarily vegetarian (∼80% of stomach content being fruits and seeds), whereas Mus musculus consumes a large amount of arthropods (∼50% arthropods, ∼30% fruits and seeds) . A mean δ13C value in bone collagen of Rattus rattus is more negative than the more insectivorous Mus musculus by ∼1.4‰ . Non-photosynthetic plant tissues are slightly more enriched in 13C than leaves . Badeck et al.  documented that roots and woody stems are isotopically heavier than leaves by 1.1‰ and 1.9‰ on average in C3 plants, respectively, whereas the differences between roots and leaves are not significant in C4 plants. Fruits and seeds are also more enriched than leaves. The isotopic differences between fruits and leaves (Δ13C fruits-leaves) range from 0.5‰ to 3.0‰ in various species of leguminous plants , .
By 7.4 Ma, the difference in mean δ13C values between large (Karnimata sp.) and small (Mus sp.) species increased significantly, reaching 3.5‰ (Figure 2 and Table S6). The difference of 3.5‰ is too great to be explained solely by isotopic variation in C3 plants or physiological variation in murine species and therefore indicates that consumption of C4 grasses had come to play an important role in isotopic differentiation among coexisting species. If the difference arose solely from proportional contribution of C4 to C3 diets, we conclude that Karnimata sp. preferentially consumed C4 plants over C3 green leaves 20 to 30% more than Mus sp. at 7.4 Ma. The isotopic difference of 4.3‰ at 6.5 Ma, between Parapelomys robertsi and Karnimata huxleyi, translates into 30 to 40% more consumption of C4 grasses by K. huxleyi.
Comparisons with Dental Morphology
The tooth size of each individual is expressed as a natural logarithm of tooth area, Ln(length*width), in Figures 2 and 3A, B (Table S7). Two sympatric species (or morphotypes) can be recognized as early as 11.2 Ma based on dental morphology of M1 . These species greatly overlap in size and morphology of m1. They are tightly clustered by carbon isotope composition (Figure 1, Table 1), indicating that these species of similar size did not isotopically partition their diets. This condition lasted at least until 10.1 Ma. Size divergence occurred by 9.2 Ma (Figure 3A, B). Compared to corresponding species at 8.2 Ma, by 7.4 Ma, Progonomys sp. became larger, while Karnimata sp. reduced its size, resulting in opening morphospace for small Mus sp. and large Parapelomys sp. The species of Mus sp. was a major species at 7.4 Ma (Figure 3A). The presence of three major species (P. robertsi, K. huxleyi, M. auctor) continued through 6.5 Ma (Table S1).
(A) Ln(length*width of m1). Symbols in A: 13.8 to 13 Ma, inverted orange triangle for Antemus chinjiensis; 11.6 to 11.2 Ma, purple diamond for Progonomys hussaini +?Karnimata sp.; 10.5 to 10.1 Ma, blue cross for Karnimata sp. + Progonomys sp.; 8.8 Ma, green cross for morphotype 7; 7.8 Ma, green cross for morphotype 8; 7.4 Ma, light blue square for Parapelomys sp.; other symbols as in Figure 2. (B) 20th percentile and 80th percentile of Ln(length*width of m1). (C) van Dam's  index of M1, calculated as tooth width divided by the distance (lap in figure) between the posterior side of the lingual anterocone and that of protocone. The vertical bar at 2.2 shows the lower boundary of VD index to be predominantly grazers. Symbols in C: 12.4 Ma, purple diamond for near Progonomys sp.; 11.6 to 11.2 Ma, purple diamond for Progonomys hussaini; 11.2 Ma, blue circle for ?Karnimata sp.; 10.5 and 10.1 Ma, blue circle for Karnimata sp., red triangle for Progonomys sp.; 8.8 and 8.2 Ma, blue circle for Karnimata sp. (+ large Karnimata sp.); other symbols as in A. (D) Hypsodonty of M1, calculated as crown height divided by length. Symbols as in C. Error bars indicate 95% bootstrap confidence intervals. Red dotted lines connect two reference points, and blue dotted lines are parallel to the red line and have one reference point.
Among coexisting species that differ in size, large species consistently have greater VD index and higher hypsodonty values (i.e., narrower valleys between anteroposteriorly aligned cusps on higher-crowned teeth) than small species from 9.2 through 6.5 Ma (Figures 3C, D, Tables S8 and S9). Karnimata sp. progressively narrowed valleys between anteroposteriorly aligned cusps through the evolutionary sequence, indicating that they consumed greater amounts of tough diets through time. In the VD index, Karnimata sp. exceeds the grazing value of 2.2 by 7.4 Ma, and large species (Parapelomys robertsi and Karnimata huxleyi) are above that value at 6.5 Ma (Figure 3C). In Karnimata, the attainment of the VD value signaling consumption of grass diets corresponds in time to the increase of the mean δ13C value and increased total range of δ13C values at 7.4 Ma. On the other hand, the “Progonomys clade” did not modify the valley space between cusps as shown by a constant VD index value even though the rate of dietary change toward more tough diets is same as Karnimata. At 7.4 Ma, Mus sp. has a mean VD value (1.77) significantly lower than that of Progonomys sp (1.96). Although the rates of change in δ13C values are the same in both clades, the different rates of morphological evolution in VD index indicate that selection pressures leading to the morphological change are greater in Karnimata than in the “Progonomys clade”.
There is no evidence of increasing crown height in Karnimata and the “Progonomys clade”, but Parapelomys robertsi, which is a derived member of the Karnimata clade , has significantly higher tooth crowns than the coexisting species at 6.5 Ma (p≤0.01 for each pair in the Games-Howell test). Thus, in Siwalik murines, tooth crown height lagged attainment of the grazing threshold in the VD index by 2.7 Ma. A morphological trend toward increasing VD index and hypsodonty values was also recognized in a clade of European murine rodents, Progonomys-Occitanomys-Stephanomys .
Contradictions in Carbon Isotope and Dental Morphology of P. robertsi
At 6.5 Ma, the largest species, Parapelomys robertsi, is more depleted in 13C than its two coexisting species (Mus auctor, Karnimata huxleyi), which would indicate a higher proportion of C3 plants in the diet. However, P. robertsi is expected to be a grazer based on dental characters. Its VD index value (2.5) is equivalent to that of Recent Golunda ellioti (Table S8), which predominantly consumes C4 grass. Its tooth crown is higher than any other Siwalik murines (Figure 3D and Table S9). Similar apparent contradictions in dental morphology and isotopic indicators of diet can be found in modern rodents. Recent Millardia sp. shows more negative δ13C values than Recent Mus booduga and Mus saxicola, but dental characters of Millardia sp. appear to be more adapted to a grass diet than for the two species of Mus (Tables S8 and S9). Shiels  reported that Rattus rattus has more negative δ13C values than coexisting Mus musculus although Rattus has greater values in VD index than Mus (Table S8). Among large herbivorous mammals, hypsodont ungulate species are not universally specialized for consuming C4 plants , . Feranec ,  proposed that hypsodonty may broaden the dietary niche of generalists feeding on mixed C3/C4 diets, which may be the case for murine rodents. Further studies of isotopic dietary inference are necessary to understand the ecomorphological strategy of small mammals. However, taxonomically fine-scale comparisons of carbon isotope ratios with grazing indices throughout the temporally well-constrained sequence of Siwalik fossil localities has provided a more detailed and calibrated view of rodent ecomorphology and dietary change than previously possible.
Isotope compositions in a sequence of molars from m1 to m3 from three mandibles (#1 to #3) of Recent Rattus sp. (A) δ13C data. (B) δ18O data. Specimens were analyzed in the same analytical run except m1 of #1 and #3, which were run four days after the others. All were right molars except m2 of #2.
SEM images of m1 of laboratory Mus musculus, which are treated by 0.1 M acetic acid. The m1specimens were provided by the University of Texas, Southwestern Medical Center. Individuals were sacrificed for a research purpose unrelated to this study.
Scatter plot of δ13C data vs. time, ranging from 9.2 to 6.5 Ma, between Karnimata and the “Progonomys clade”.
Scatter plot of van Dam's  index vs. time, ranging from 9.2 to 6.5 Ma, between Karnimata and the “Progonomys clade”.
Scatter plot of hypsodonty vs. time, ranging from 9.2 to 6.5 Ma, between Karnimata and the “Progonomys clade”.
Full data of m1 specimens analyzed in this study, including dental measurements, δ13C values, and the side of teeth from which isotope data were taken. The δ13C values are corrected to be consistent with the H3PO4 method using the laser-H3PO4 offset of modern beaver incisor in every run. Specimens from YGSP 182 treated by 0.1 M acetic acid instead of 0.1 M sodium acetate-acetic acid buffer are marked in Acetic acid.
Full data of M1 specimens of Siwalik murines from the Potwar Plateau, including dental measurements, van Dam's  index, and hypsodonty values. Abbreviations: lap, distance between the posterior side of the lingual anterocone and that of protocone (see Figure 3C); VD, van Dam's  index; Hy, hypsodonty.
Key to names of Siwalik murine species used in this study in comparison to Jacobs and Flynn . Major species are those known by five or more specimens of the upper first molars (M1). Minor species are those known by less than five specimens of M1.
Carbon isotope data summarized by age. Note that all data but YGSP 34415 are included in the descriptive statistics.
Results of ANCOVA between Karnimata and the “Progonomys clade”. Asterisks for p<0.05.
Summary of linear regression analysis for δ13C values, VD index, and hypsodonty. Asterisks for p<0.05.
Results of statistical tests for carbon isotope data in coexisting species at 7.4 Ma, 6.5 Ma, and Recent. Asterisks for p<0.05. Abbreviations: WA, Welch's ANOVA; MW, Mann-Whitney U test.
Results of the Games-Howell post-hoc test, associated with Table S5. The mean difference is expressed as a mean of species 1 minus that of species 2. Asterisks for p<0.05.
Summary of dental measurements of m1 in mm.
Means of VD index with 95 % bootstrap confidence intervals.
Means of hypsodonty measurements with 95 % bootstrap confidence intervals. Asterisks indicate hypsodonty values measured in specimens in wear stage III of Lazzari et al. .
Effect of nursing on carbon isotope composition in murine rodents.
We sincerely thank J. Prange-Kiel, D. Dearing, and A. Winkler for providing specimens of laboratory mice and field-captured rodents. We also acknowledge C. Badgley for letting us access compiled data of carbon isotope ratios in large mammals and soil carbonates and R. Beavers for SEM images. We are thankful to the editor L. Viriot and reviewers (J.-J. Jaeger and one anonymous reviewer) for providing useful comments that considerably improved the manuscript.
Conceived and designed the experiments: YK LLJ TEC. Performed the experiments: YK KTU. Analyzed the data: YK LLJ TEC KTU KMF LJF. Contributed reagents/materials/analysis tools: LLJ TEC LJF RP. Wrote the paper: YK LLJ TEC KTU KMF LJF RP.
- 1. Lee-Thorp JA, van der Merwe NJ (1987) Carbon isotope analysis of fossil bone apatite. S Afr J Sci 83: 712–715.
- 2. Cerling TE, Harris JM (1999) Carbon isotope fractionation between diet and bioapatite in ungulate mammals and implications for ecological and paleoecological studies. Oecologia 120: 347–363.
- 3. Koch PL (1998) Isotopic reconstruction of past continental environments. Annu Rev Earth Planet Sci 26: 573–613.
- 4. Kohn MJ, Cerling TE (2002) Stable isotope compositions of biological apatite. Rev Mineral Geochem 48: 455–488.
- 5. Cerling TE, Harris JM, Leakey MG, Mudida N (2003) Stable isotope ecology of Northern Kenya, with emphasis on the Turkana Basin. In: Leakey MG, Harris JM, editors. Lothagam: The Dawn of Humanity in East Africa: Columbia University Press. 583–603.
- 6. Feranec RS (2003) Stable isotopes, hypsodonty, and the paleodiet of Hemiauchenia (Mammalia: Camelidae): a morphological specialization creating ecological generalization. Paleobiology 29: 230–242.
- 7. MacFadden BJ (1997) Origin and evolution of the grazing guild in New World terrestrial mammals. Trends Ecol Evol 12: 182–187.
- 8. Grimes ST, Collinson ME, Hooker JJ, Mattey DP (2008) Is small beautiful? A review of the advantages and limitations of using small mammal teeth and the direct laser flourination analysis technique in the isotope reconstruction of past continental climate change. Palaeogeogr Palaeoclimatol Palaeoecol 266: 39–50.
- 9. Gehler A, Tütken T, Pack A (2012) Oxygen and carbon isotope variations in a modern rodent community – implications for palaeoenvironmental reconstructions. PLoS ONE 7: e49531.
- 10. Hopley PJ, Latham AG, Marshall JD (2006) Paleoenvironments and palaeodiets of mid-Pliocene micromammals from Makapansgat Limeworks, South Africa: A stable isotope and dental microwear approach. Palaeogeogr Palaeoclimatol Palaeoecol 233: 235–351.
- 11. Hynek SA, Passey BH, Prado JL, Brown FH, Cerling TE, et al. (2012) Small mammal carbon isotope ecology across the Miocene–Pliocene boundary, northwestern Argentina. Earth Planet Sci Lett 321–322: 177–188.
- 12. Yeakel JD, Bennett NC, Koch PL, Dominy NJ (2007) The isotopic ecology of African mole rats informs hypotheses on the evolution of human diet. Proc R Soc B 274: 1723–1730.
- 13. Grimes ST, Mattey DP, Hooker JJ, Collinson ME (2003) Paleogene paleoclimate reconstruction using oxygen isotopes from land and freshwater organism: the use of multiple paleoproxies. Geochim Cosmochim Acta 67: 4033–4047.
- 14. Grimes ST, Collinson ME, Hooker JJ, Mattey DP, Grassineau NV, et al. (2004) Distinguishing the diets of coexisting fossil theridomyid and glirid rodents using carbon isotopes. Palaeogeogr Palaeoclimatol Palaeoecol 208: 103–119.
- 15. McLean BS, Emslie SD (2012) Stable isotopes reflect the ecological stability of two high-elevation mammals from the late Quaternary of Colorado. Quatern Res 77: 408–417.
- 16. Rogers KL, Wang Y (2002) Stable isotopes in pocket gopher teeth as evidence of a Late Matuyama climate shift in the Southern Rocky mountains. Quatern Res 57: 200–207.
- 17. Passey BH, Cerling TE (2006) In situ stable isotope analysis (δ13C, δ18O) of very small teeth using laser ablation GC/IRMS. Chem Geol 235: 238–249.
- 18. Quade J, Cerling TE, Bowman JR (1989) Development of Asian monsoon revealed by marked ecological shift during the latest Miocene in northern Pakistan. Nature 342: 163–166.
- 19. Cerling TE, Harris JM, MacFadden BJ, Leakey MG, Quade J, et al. (1997) Global vegetation change through the Miocene/Pliocene boundary. Nature 389: 153–158.
- 20. Fox DL, Koch PL (2003) Tertiary history of C4 biomass in the Great Plains, USA. Geology 31: 809–812.
- 21. Fox DL, Koch PL (2004) Carbon and oxygen isotopic variability in Neogene paleosol carbonates: constraints on the evolution of the C4-grasslands of the Great Plains, USA. Palaeogeogr Palaeoclimatol Palaeoecol 207: 305–329.
- 22. Latorre C, Quade J, McIntosh WC (1997) The expansion of C4 grasses and global change in the late Miocene: Stable isotope evidence from the Americas. Earth Planet Sci Lett 146: 83–96.
- 23. Badgley C, Barry JC, Morgan ME, Nelson SV, Behrensmeyer AK, et al. (2008) Ecological changes in Miocene mammalian record show impact of prolonged climatic forcing. Proc Natl Acad Sci USA 105: 12145–12149.
- 24. Jacobs LL, Flynn LJ, Downs WR (1990) Quo vadis, Antemus? The Siwalik muroid record. In: Lindsay EH, editor. European Neogene Mammal Chronology. New York: Plenum Press. 573–586.
- 25. Musser GG, Carleton MD (2005) Superfamily Muroidea. In: Wilson DE, Reeder DM, editors. Mammal species of the World: a taxonomic and geographic reference. 3rd ed. Baltimore: Johns Hopkins University Press. 894–1531.
- 26. Jacobs LL, Flynn LJ, Downs WR (1989) Neogene rodents of southern Asia. In: Black CC, Dawson MR, editors. Papers on Fossil Rodents in Honor of Albert E Wood: Natural History Museum of Los Angeles County, Science Series. 157–177.
- 27. Jacobs LL (1978) Fossil rodents (Rhizomyidae and Muridae) from Neogene Siwalik deposits, Pakistan. Mus Northern Arizona Press Bull Ser 52: 1–103.
- 28. Jacobs LL, Downs WR (1994) The evolution of murine rodents in asia. Nat Sci Mus Monogr 8: 149–156.
- 29. Jacobs LL, Flynn LJ (2005) Of Mice…again: the Siwalik rodent record, murine distribution, and molecular clocks. In: Lieberman DE, Smith RJ, Keller J, editors. Interpreting the Past Essays on Human, Primate, and Mammal Evolution in Honor of David Pilbeam: Brill Academic Publishers. 63–80.
- 30. van Dam JA (1997) The small mammals from the Upper Miocene of the Teruel–Alfambra region (Spain): paleobiology and paleoclimatic reconstructions. Geol Ultraiectina 156: 1–204.
- 31. Patnaik R (2001) Late Pliocene micromammals from Tatrot Formation (Upper Siwaliks) exposed near village Saketi, Himachal Pradesh, India. Palaeontogr Abt A 261: 55–81.
- 32. Patnaik R (1997) New murids and gerbillids (Rodentia, Mammalia) from Pliocene Siwalik sediments of India. Palaeovertebrata 26: 129–165.
- 33. Jacobs LL (1977) A new genus of murid rodent from the Miocene of Pakistan and comments on the origin of the Muridae. PaleoBios 25: 1–11.
- 34. Cheema IU, Raza SM, Flynn LJ, Rajpar AR, Tomida Y (2000) Miocene small mammals from Jalalpur, Pakistan, and their biochronologic implications. Bull Natl Sci Mus Ser C 26: 57–77.
- 35. Lazzari V, Charles C, Tafforeau P, Vianey-Liaud M, Aguilar J-P, et al. (2008) Mosaic convergence of rodent dentitions. PLoS ONE 3: e3607.
- 36. Quade J, Cerling TE, Barry JC, Morgan ME, Pilbeam DR, et al. (1992) A 16-Ma record of paleodiet using carbon and oxygen isotopes in fossil teeth from Pakistan. Chem Geol 94: 183–192.
- 37. Craig H (1954) Carbon 13 in plants and the relationships between carbon 13 and carbon 14 variations in nature. J Geol 62: 115–149.
- 38. Passey BH, Robinson TF, Ayliffe LK, Cerling TE, Sponheimer M, et al. (2005) Carbon isotope fractionation between diet, breath CO2, and bioapatite in different mammals. J Archaeol Sci 32: 1459–1470.
- 39. Podlesak DW, Torregrossa A-M, Ehleringer JR, Dearing MD, Passey BH, et al. (2008) Turnover of oxygen and hydrogen isotopes in the body water, CO2, hair, and enamel of a small mammal. Geochim Cosmochim Acta 72: 19–35.
- 40. Iacumin P, Bocherens H, Mariotti A, Longinelli A (1996) Oxygen isotope analyses of co-existing carbonate and phosphate in biogenic apatite: a way to monitor diagenetic alteration of bone phosphate? Earth Planet Sci Lett 142: 1–6.
- 41. Marino BD, McElroy MB, Salawitch RJ, Spaulding WG (1992) Glacial-to-interglacial variations in the carbon isotopic composition of atmospheric CO2. Nature 357: 461–466.
- 42. Francey RJ, Allison CE, Etheridge DM, Trudinger CM, Enting IG, et al. (1999) A 1000-year high precision record of δ13C in atmospheric CO2. Tellus 51B: 170–193.
- 43. Keeling RF, Piper SC, Bollenbacher AF, Walker SJ (2010) Monthly atmospheric 13C/12C isotopic ratios for 11 SIO stations. Trends: A Compendium of Data on Global Change: Carbon Dioxide Information Analysis Center, Oak Ridge National Laboratory, U.S. Department of Energy. Available: http://cdiac.ornl.gov/trends/co2/iso-sio/iso-sio.html. Accessed 15 July 2012.
- 44. Koch PL, Tuross N, Fogel ML (1997) The effects of sample treatment and diagenesis on the isotopic integrity of carbonate in biogenic hydroxylapatite. J Archaeol Sci 24: 417–429.
- 45. Patnaik R (2003) Reconstruction of Upper Siwalik palaeoecology and palaeoclimatology using microfossil palaeocommunities. Palaeogeogr Palaeoclimatol Palaeoecol 197: 133–150.
- 46. Fortelius M (1985) Ungulate cheek teeth: developmental, functional, and evolutionary interrelations. Acta Zool Fennica 180: 1–76.
- 47. Damuth J, Janis CM (2011) On the relationship between hypsodonty and feeding ecology in ungulate mammals and its utility in paleoecology. Biol Rev 86: 733–758.
- 48. Janis CM (1988) An estimation of tooth volume and hypsodonty indices in ungulate mammals, and the correlation of these factors with dietary preference. In: Russell DE, Santoro J-P, Sigogneau-Russell D, editors. Teeth revisited: Proceedings of the VIIth International Symposium on Dental Morphology, Paris: Mémoirs de Musée d'Histoire naturelle. 367–387.
- 49. Lecompte E, Aplin K, Denys C, Catzeflis F, Chades M, et al. (2008) Phylogeny and biogeography of African Murinae based on mitochondorial and nuclear gene sequences, with a new tribal classification of the subfamily. BMC Evol Biol 8: 199.
- 50. Nowak RN (1999) Walker's Mammals of the World. Baltimore: Johns Hopkins Press. 1936 p.
- 51. Canty A, Ripley B (2012) boot: Bootstrap R (S-Plus) Functions. R package version 137.
- 52. SPSS Inc. (2009) PASW Statistics for Windows, version 18.0. Chicago: SPSS Inc.
- 53. R Development Core Team (2012) A language and environment for statistical computing. Vienna: R Foundation for Statistical Computing.
- 54. Codron J, Codron D, Lee-Thorp JA, Sponheimer M, Bond WJ, et al. (2005) Taxonomic, anatomical, and spatio-temporal variations in the stable carbon and nitrogen isotopic compositions of plants from an African savanna. J Archaeol Sci 32: 1757–1772.
- 55. McCutchan JHJ, Lewis WMJ, Kendall C, McGrath CC (2003) Variation in trophic shift for stable isotope ratios of carbon, nitrogen, and sulfur. Oikos 102: 378–390.
- 56. Badeck F-W, Tcherkez G, Nogués S, Piel C, Ghashghaie J (2005) Post-photosynthetic fractionation of stable carbon isotopes between plant organs–a widespread phenomenon. Rapid Commun Mass Spectrom 19: 1381–1391.
- 57. Behrensmeyer AK, Quade J, Cerling TE, Kappelman J, Khan IA, et al. (2007) The structure and rate of late Miocene expansion of C4 plants: Evidence from lateral variation in stable isotopes in paleosols of the Siwalik Group, northern Pakistan. Geol Soc Am Bull 119: 1486–1505.
- 58. Shiels AB (2010) Ecology and impacts of introduced rodents (Rattus spp. and Mus musculus) in the Hawaiian Islands. PhD thesis, University of Hawaii. Available: http://manoa.hawaii.edu/hpicesu/DPW/AS_THESIS/04.pdf. Accessed 15 June 2012.
- 59. Cernusak LA, Tcherkez G, Keitel C, Cornwell WK, Santiago LS, et al. (2009) Why are non-photosynthetic tissues generally 13C enriched compared with leaves in C3 plants? Review and synthesis of current hypotheses. Funct Plant Biol 36: 199–213.
- 60. Yoneyama T, Ohtani T (1983) Variations of natural 13C abundances in leguminous plants. Plant Cell Physiol 24: 971–977.
- 61. Cernusak LA, Pate JS, Farquhar GD (2002) Diurnal variation in the stable isotope composition of water and dry matter in fruiting Lupinus angustifolius under field conditions. Plant Cell Environ 25: 893–907.
- 62. Feranec RS (2007) Ecological generalization during adaptive radiation: evidence from Neogene mammals. Evol Ecol Res 9: 555–577.
- 63. Ogg JG, Smith AG (2004) The geomagnetic polarity time scale. In: Gradstein FM, Ogg JG, Smith AG, editors. A Geologic Time Scale 2004. Cambridge, UK: Cambridge University Press. 63–86.