Listeria monocytogenes, a foodborne bacterial pathogen, is comprised of four phylogenetic lineages that vary with regard to their serotypes and distribution among sources. In order to characterize lineage-specific genomic diversity within L. monocytogenes, we sequenced the genomes of eight strains from several lineages and serotypes, and characterized the accessory genome, which was hypothesized to contribute to phenotypic differences across lineages. The eight L. monocytogenes genomes sequenced range in size from 2.85–3.14 Mb, encode 2,822–3,187 genes, and include the first publicly available sequenced representatives of serotypes 1/2c, 3a and 4c. Mapping of the distribution of accessory genes revealed two distinct regions of the L. monocytogenes chromosome: an accessory-rich region in the first 65° adjacent to the origin of replication and a more stable region in the remaining 295°. This pattern of genome organization is distinct from that of related bacteria Staphylococcus aureus and Bacillus cereus. The accessory genome of all lineages is enriched for cell surface-related genes and phosphotransferase systems, and transcriptional regulators, highlighting the selective pressures faced by contemporary strains from their hosts, other microbes, and their environment. Phylogenetic analysis of O-antigen genes and gene clusters predicts that serotype 4 was ancestral in L. monocytogenes and serotype 1/2 associated gene clusters were putatively introduced through horizontal gene transfer in the ancestral population of L. monocytogenes lineage I and II.
Citation: den Bakker HC, Desjardins CA, Griggs AD, Peters JE, Zeng Q, Young SK, et al. (2013) Evolutionary Dynamics of the Accessory Genome of Listeria monocytogenes. PLoS ONE 8(6): e67511. https://doi.org/10.1371/journal.pone.0067511
Editor: Catherine A. Brissette, University of North Dakota School of Medicine and Health Sciences, United States of America
Received: July 24, 2012; Accepted: May 23, 2013; Published: June 25, 2013
Copyright: © 2013 den Bakker et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This project has been funded in whole or in part with Federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under Contract No.: HHSN272200900018C. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
In this study we focus on the evolution and dynamics of the accessory genome of the foodborne pathogen Listeria monocytogenes. L. monocytogenes is a saprotrophic Firmicute, which can be commonly found in the environment. In case of an (usually foodborne) infection of a susceptible host, it switches from a saprotrophic to an intracellular pathogenic lifestyle and can cause a severe systemic infection termed listeriosis . Current population genetic and phylogenetic data show that L. monocytogenes can be subdivided into four phylogenetic lineages, designated Lineage I, II, III and IV, which seem to differ in ecology, recombination rates and genomic content . Lineage I strains seem to be overrepresented among human clinical cases in many countries, while lineage II strains are common in foods and seem to be widespread in natural and farm environments . Lineage III and IV strains are rare among human clinical cases and in foods compared to strains of the other lineages and have been associated with animal clinical cases .
Traditional subtyping of L. monocytogenes has relied on serotyping . L. monocytogenes serotypes are predominantly determined by somatic (O-) antigens, with 12 recognized O-antigens, which are highly variable between serotypes. Flagellar (H-) antigens are less abundant (only four antigens in L. monocytogenes) and are conserved in the majority of the L. monocytogenes serotypes . Serotypes 4b and 1/2b are the dominant serotypes in lineage I, while serotypes 1/2a and 3a are the most common serotypes in lineage II , . Lineages III and IV contain the serotypes 4a, 4b and 4c . O-antigenic variation is correlated to the biochemistry of the wall teichoic acids, components of the cell wall, which are exposed to the external milieu –. In particular the decoration of the wall teichoic acids seems to be correlated to serotype, ranging from no decoration in serotype 7, rhamnose based decorations in serotypes 1/2, and glucose and galactose based decorations in variants of serotypes 4, 5 and 6 , . To our knowledge, only rhamnose has so far been proven experimentally to be a major antigenic determinant, for serotype 1/2a . The major antigenic determinants for the other serotypes still have to be experimentally confirmed. The prevailing hypothesis based on population genetic research of L. monocytogenes has been that the most recent common ancestor of L. monocytogenes had a 1/2b serotype, and that the 4b serotype arose only recently from a 1/2b ancestor , . The number of serotypes recognized within L. monocytogenes is very small as compared to pathogens such as Salmonella enterica, which has more than 2,600 distinct serotypes , . This suggests that cell surface-related proteins responsible for antigen variation in L. monocytogenes may be under less diversifying selection as compared to other pathogens.
Comparative genomic research on L. monocytogenes has previously focused on pan/core genome size estimates and the role of recombination and positive selection in the evolution of the core genome –. The pan-genome (the collection of all genes) of a given bacterial taxonomical unit (TU; usually a species or genus) can be subdivided into the accessory genome (the collection of genes found in a subset of strains but not all strains of the TU) and the core genome (the collection of genes found among all strains of the TU). The core genome can be used to identify the specific genomic characteristics of a given TU, while the size, content and dynamics of the accessory genome can be an indicator of the plasticity or adaptability of a given TU . The accessory genome of different populations within a bacterial species can differ significantly due to selective pressures experienced in different environments . Therefore knowledge of the content and dynamics of the accessory genome of individual populations within a species may elucidate the kind of selective pressures experienced by these populations and increase our understanding of the ecology of a species. Here, we sequenced the genomes of 8 strains of L. monocytogenes, including representatives of lineages I, II, and III, and previously unsequenced serotypes 3a and 1/2c. We used these data to characterize the evolutionary dynamics of the accessory genome of L. monocytogenes to gain a better understanding of the genome organization of this pathogen and further focus on the evolution of O-antigen associated genes.
Materials and Methods
Bacterial Strains and Genome Sequencing
Bacterial strains used in this analysis and basic assembly information of each strain can be found in Table 1. In addition to the newly sequenced genomes presented in this paper we added representative published genomes – to the analysis. Sanger sequences were generated from three whole genome shotgun sequencing libraries for each strain (two plasmid libraries (4 kb and 10 kb inserts) and a Fosmid library (40 kb inserts)), using ABI 3730 machines as described previously . The remaining sequences were generated using 454  and Illumina  technology. Genome sequences of strains J0161, 10403S, FSL R2-561, and Finland 1998 were assembled with HybridAssemble from the September 2008 version of the Arachne assembly package  using both Sanger and 454 sequences. Assemblies for the other strains were created with Newbler 1.1.03.19 (http://454.com/products/analysis-software/index.asp) using 454 data and were then improved using SolexaPoly (from the September 2008 Arachne assembly package), which uses Illumina sequence data to correct 454 errors.
Classical serotyping was performed for a select number of isolates of Listeria innocua (FSL S4-378, FSL J1-023), Listeria seeligeri (FSL N1-067, FSL S4-171) and L. marthii (FSL S4-120), using antisera from Denka Seiken (Denka Seiken Co Ltd, Tokyo, Japan).
Protein-coding genes were predicted using a combination of ab initio, synteny-based, and homology-based gene prediction methods. For ab initio gene predictions, ORFs were predicted using Glimmer3 with default parameters , MetaGene with default parameters , and GeneMark trained with the 500 longest ORFs predicted by Glimmer3 . Synteny-based gene prediction was conducted as previously described , using default parameters for both Nucmer  and LAGAN  alignments and strains EGD-e and F2365 as reference genomes. In regions without ab initio or synteny-based gene models, homology-based gene models were constructed from BLAST hits to the non-redundant protein database with an e-value cutoff of 1×10−10. Gene product names were assigned based on BLAST hits to the UniRef90 database and hmmer hits to TIGRfam and PFAM, and every gene was assigned a unique locus number of the form xxxG_#####. Ribosomal RNAs were identified with RNAmmer , tRNA features were identified using tRNAScan , and other non-coding features were identified with RFAM .
Gene Ontology and Enrichment Analysis
Overrepresentation (enrichment) of certain Gene Ontology (GO) categories in the core versus the accessory genome and in the region around the chromosomal origin of replication was tested using a Bonferroni corrected Fisher’s exact test. Gene Ontology terms were assigned to each gene using Blast2GO  with an e-value cutoff of 1×10−10.
Gene Clustering and Evolutionary Analyses
Orthology assignment was performed with OrthoMCL 1  with a Markov inflation index of 1.5 and a maximum e-value of 1e-5, using the default parameter settings. We defined core genes as those present in all 10 finished genomes and accessory genes as those missing from at least 1 finished genome. Sequences of these clusters were aligned using MUSCLE , poorly aligned regions were trimmed using trimAl under default settings , and individual gene phylogenies were estimated using FastTree . We then calculated dN/dS for each cluster using the CODEML program of the PAML package (version 4.4) using the model of a single omega for all branches . To generate an organismal phylogeny we concatenated alignments of the 2,086 genes that were present as single copies in all genomes, and estimated a phylogeny using the GTRMIX model in RAxML . The tree was made ultrametric using PathD8  for ease of visualization.
Insertion/Deletion Hot Spot Analyses
Insertion/deletion hotspot maps were created as described in Touchon et al. . In short, genes present in all strains (single copy core genes) were plotted on the X-axis, while genes that were present in the insertion/deletion regions (the accessory genome) were plotted on the y-axis at the position relative to the adjacent core genome genes. To test if two groups (L. monocytogenes vs Staphylococcus aureus) have different accessory gene distributions across the chromosome, we plotted the cumulative distribution of accessory genes over the chromosome. Positions of the accessory genes on the genome were transformed to degrees (following the formula given in ) to allow comparisons of the distribution of the accessory genome among genomes, even in the presence of frequent genome rearrangements. Prophage related genes were excluded from this analysis. We then identified the degree position that divided the genome into two regions and maximized the χ2 value of the difference in the distributions of core and accessory genes.
Evolution of Genes Associated with O-antigen Variation
Genes with phylogenetic histories discordant with the major lineage divisions were identified using a previously described method . Briefly, each gene was assigned a value based on its position within the phylogenetic tree of its orthologs within L. monocytogenes. These values were mapped to a gradient ranging from dark red (groups solely with lineage I, III, and IV) to dark blue (groups solely with lineage II). Then each gene of each genome was plotted by color against the reference genome of strain F2365, using Circos . Nucleotide sequences of genes from discordant regions in L. monocytogenes, along with genes from additional Listeria species (L. innocua CLIP11262 serotype 6a, FSL S4-378 serotype 4ab, FSL J1-023 serotype 4b; L. seeligeri SLCC3954 serotype 1/2b, FSL S4-171 serotype 4c, FSL N1-067 serotype 7; L. marthii FSL S4-120 6a; L. ivanovii subsp. ivanovii PAM and L. ivanovii subsp. londoniensis; both serotype 5) were aligned using MUSCLE version 3.8.31 . Phylogenetic trees were inferred from these alignments using the maximum likelihood criterion in PHYML version 3 , with 100 bootstrap replicates. Maximum likelihood trees were inspected and categorized into two groups; (i) trees primarily clustering according to the organismal tree (that is the phylogenetic relationships are congruent to the inter- and intraspecific phylogenies of Listeria as inferred in ) and (ii) trees that cluster according to serotype. To reconcile individual gene trees with the organismal tree, AnGST  (http://almlab.mit.edu/angst/) and Mowgli  (http://www.atgc-montpellier.fr/Mowgli/) were used. AnGST was run using the event penalties recommended by the authors of the software (horizontal gene transfer: 3, gene duplication: 2, gene loss: 1, and speciation: 0), Mowgli was ran using the default parameters, with the exception that nearest neighbor editing was allowed for branches with a bootstrap support <60.
Recombination Analysis Tool (RAT: ) was used to detect putative recombination breakpoints in gene clusters.
L. monocytogenes Genomes are Highly Conserved
We sequenced the genomes of eight strains of L. monocytogenes (Table 1), yielding three finished (single scaffold) and five high-quality draft (coverage ≥20X, multiple scaffolds) genomes. Furthermore, we generated improved assemblies for four previously published genomes , resulting in one additional finished and three high-quality draft genomes. All genomes were annotated (see methods) and resulting statistics are shown in Table 2. In Table 1, we compare these genomes to an additional six finished and three annotated draft genomes already available in Genbank. Genome size in L. monocytogenes genomes is tightly conserved, ranging from the 2.74 Mb genome of FSL J1-208 to the 3.14 Mb genome of FSL N1-017, and is not correlated with lineage membership. As expected, the largest and smallest genomes also had the fewest and most genes, 2,765 and 3,187, respectively.
OrthoMCL  was used to identify clusters of orthologous genes across all Listeria genomes. We identified 2,439 L. monocytogenes core genes present in all 10 completely sequenced genomes, similar to previously estimated size (between 2,330 and 2,465 genes) of the core genome of L. monocytogenes , . The accessory genome represents a small fraction of L. monocytogenes gene content relative to the core genomes (12–23%). Therefore, while there is substantial variation in the size of the accessory genome (which ranges from 323 to 753 genes per strain), genome size and the total number of genes are highly conserved across L. monocytogenes strains. Variation in accessory genome size can be due to many factors, including biological factors such as the presence/absence of various prophages in the L. monocytogenes genomes (as previously shown in Den Bakker et al. ) or artifacts such as completeness of the genome assemblies.
The First 65 Degrees Adjacent to the Origin of Replication of L. monocytogenes are Significantly Enriched for Accessory Genes
Utilizing the 2,086 genes identified as orthologous across all Listeria species, we constructed a phylogeny of L. monocytogenes genomes rooted with outgroup genomes of the closely related species L. innocua, L. welshimeri, and L. seeligeri (Fig. 1, outgroups not shown). This phylogeny agrees with previous phylogenetic analyses ,  and divides L. monocytogenes into its four major lineages. To examine the positioning of the accessory genes along L. monocytogenes genomes in a phylogenetic context, the number of accessory genes between each core gene was plotted for each genome (Fig. 1). Positioning of accessory gene clusters is conserved across L. monocytogenes genomes, as was observed by Touchon et al. in E. coli . The distribution of accessory gene clusters over the chromosome in L. monocytogenes seems to differ from that of E. coli in that in L. monocytogenes there is a high concentration of these accessory gene clusters close to the origin of replication. This is particularly true in the region spanning the first approximately 500 Kb of the chromosome. While this paper was under review, Kuenne et al.  published an analysis of accessory gene distribution using a largely non-overlapping set of L. monocytogenes strains, and also found genes clustered into insertion-deletion hotspots. Independent confirmation of these insertion-deletion hotspots in different sets of genomes by Kuenne et al.  and this study show that these hotspots are highly conserved among L. monocytogenes strains. Concentration of hotspots to the right of the origin of replication is also supported by Kuenne et al. , who found eight out of nine insertion deletion hotspots identified in their study to be positioned in the right replichore. In our work, however, we noted that genomic change is not restricted to these hotspots, but that the whole region of the chromosome adjacent to the origin of DNA replication is prone to insertion and deletion events (see below) and can be considered a ‘hot region’.
Plots show the number of accessory genes in between each core gene as ordered in the reference strain EGDe. Insertion sites of prophages (P), integrated conjugative elements (ICE), and Listeria genomic islands (LGI) as detailed in Table 4 are indicated above each accessory genome distribution plot. Vertical dotted lines with a question mark indicate prophages, which are not assembled in a single contiguous piece, but are hypothesized to be present in the location based on presence of the appropriate phage genes in unalignable fraction of the assembly. Plots are colored by lineage: I, red, II, blue, III, green, IV, purple. Serotypes are shown to the right of each plot. The phylogenetic tree is based on a maximum likelihood analysis of the concatenated alignments of 2,086 core genes.
To test if this distribution is uniquely found in L. monocytogenes we plotted the cumulative distribution of accessory genes along the chromosome for L. monocytogenes, and the phylogenetically closely related species Staphylococcus aureus and the Bacillus cereus group, with the exclusion of prophage regions. L. monocytogenes shows a highly unequal distribution with 38% of the accessory genes found within the first 65° (approximately a 0.5 Mb region) from the origin of replication (χ2 = 2411, p<0.0001), while the accessory genomes of S. aureus and the B. cereus group are more evenly distributed over the chromosome (Fig. 2). The distributions of accessory genes in S. aureus and B. cereus were significantly different from that of L. monocytogenes (P<0.0001, Kruskal-Wallis test), confirming the uniqueness of the pattern found in L. monocytogenes.
The circular genome position starts at the origin of replication, which is at 0 degrees.
To evaluate whether the strength of selection differs between the different regions of the genome and between core and accessory genes, we calculated dN/dS for all genes shared by at least two L. monocytogenes strains. As expected, we found that genes in the accessory genome are less selectively constrained than those in the core genome (median dN/dS = 0.131 and 0.036, respectively, p<0.001, Wilcoxon test). However we also found that core genes in the first 65° of the genome experience significantly less purifying selection than core genes in the last 295° of the genome (median dN/dS = 0.045 and 0.035, respectively, p<0.001, Wilcoxon test). The same pattern was also found for accessory genes (median dN/dS = 0.133 and 0.128, respectively, p = 0.003, Wilcoxon test). This suggests that irrespective of designation as a core or accessory, genes in the first 65° of the genome are more rapidly evolving than those in the last 295°.
We also found differences in the length of intergenic regions. Intergenic regions in the first 65° of the genome are significantly longer than intergenic regions in the last 295° of the genome (p<0.0001, Wilcoxon test). This difference in intergenic length distributions is the result of comparably long regions between neighboring accessory and core genes (median length 85 bp); intergenic regions between accessory and core genes are significantly more common in the first 65° relative to the last 295° (p<0.0001, chi-square test) of the chromosome. Interestingly, the core-core intergenic regions (median length = 45 bp) were found to be significantly longer than accessory-accessory intergenic regions (median length = 24 bp; p<0.0001, Wilcoxon test).
The Accessory Genome of L. monocytogenes is Enriched for Phosphotransferase Systems, Cell Surface Genes, and Prophages
Eight functional categories were found significantly overrepresented in the accessory genome of L. monocytogenes and were represented by more than 100 genes in each category (Table 3). These categories relate to four broad classes of genes: (i) phosphotransferase system (PTS) components (involved in sugar transport), (ii) cell wall components, (iii) transcriptional regulators (represented by the sequence-specific DNA binding term), and (iv) mobile elements (represented by the DNA integration term). The enrichment for mobile elements is likely reflective of the numerous large prophages that are unequally distributed across the different L. monocytogenes strains (Fig. 1; Table 4). The over-representation of genes corresponding to the remaining three categories likely represents a response to the diverse environmental pressures faced by L. monocytogenes.
To further examine evolutionary changes in the accessory genome, we identified accessory loci that distinguish the two major lineages of L. monocytogenes, I and II (Table 5). Lineage II has significantly more distinguishing genes than lineage I (38 vs. 21; p = 0.03, chi-square test). Most functional categories from the enrichment analysis are represented within the lineage specific operons – both lineages have specific PTS operons (including transcriptional regulators) and cell-wall anchored proteins (including internalins). Furthermore, each lineage had a specific antimicrobial resistance-related operon/gene (Table 5; lineage I, anti-microbial peptide ABC-type transport system; lineage II, bacteriocin immunity protein). Despite inclusion of only two representatives of lineage III in our analysis (HCC23 and J2-071), this lineage showed a large degree of variation with respect to presence/absence of loci it from distinguishing lineages I and II, consistent with a previous array-based study .
O-antigen Associated Genes seem to Follow a Serotype Specific Phylogenetic Pattern and show Several Instances of Horizontal Gene Transfer
A phylogenetic approach to identify genes with evolutionary histories that deviate from the organismal phylogeny identified two gene clusters: (i) a cluster corresponding to lmo1074–1091 in L. monocytogenes EGD-e (cluster 1), and (ii) a cluster (cluster 2) corresponding to lmo2549-2558 in L. monocytogenes EGD-e (Fig. 3). These clusters are found in distinct regions of the genome; however, they both contain genes implicated in the biosynthesis of wall teichoic and lipoteichoic acids. Wall teichoic acids are associated with O-antigen variation , ,  and because of this putative involvement, we will refer to these clusters as O-antigen clusters 1 and 2. For these clusters, the lineage I serotype 1/2b strains appear to have genes that are much more closely related to their orthologs in lineage II, which includes all the 1/2a and 3c strains, than to their orthologs in other lineage I strains (Fig.3). The phylogenetic distribution of serotype 1/2 related genes is incongruent with the organismal phylogeny (Fig. 1), and therefore horizontal transfer of these clusters from lineage II into lineage I could explain the occurrence of 1/2 serotypes in both lineages.
The order of genome rings is listed in the circle center, with F2365 being the outermost ring. The 7 outermost rings represent lineage I (serotype 4b and 1/2b), the next three rings represent lineage III and lineage IV strains (serotype 4a and 4c), and the last 11 rings represent lineage II strains (serotype 1/2a, 1/2c, and 3a). Clade membership of the individual genes is indicated by color; blue indicates lineage II, red indicates lineage I, and gray is unresolved membership. The two O-antigen gene clusters are highlighted in green and yellow. Genes in these clusters found in serotype 1/2b lineage I cluster phylogenetically with orthologs found in lineage II clade.
Within a serotype (1/2 or 4, irrespective of alphabetical designation), all L. monocytogenes strains have largely the same gene content and order across both clusters (Fig. 4A, Figs. S1 and S2). Exceptions are a hypothetical protein (LMOf2365_1098 in strain F2365) in cluster 1 of lineage I serotype 4b strains and the lineage IV serotype 4 strain FSL J1-208. Between serotypes, O-antigen clusters 1 and 2 substantially differ in gene content (Fig. 4A, Fig. S1 and 2). The genomes of newly sequenced serotype 3a and 1/2c strains have identical gene content in the two serotype clusters as 1/2a strains, consistent with the phylogeny based on the concatenated alignments of the 2,086 core genes, which places the serotype 3a and 1/2c genomes among lineage II 1/2a strains (Fig. 1).
The organismal phylogeny of the genus Listeria is shown in the upper panel (A), while the syntenic relationships of the two O-antigen gene clusters between the two major serotype divisions and the phylogenetic tree based on a representative serotype specific gene are shown in the two lower panels (B and C). Genes are colored by their phylogenetic histories: Serotype-specific genes (i.e., genes found only in specific serotypes) are colored green, while genes displaying an organismal phylogeny across the Listera genus are colored blue. Genes which follow a serotype-related phylogeny across Listeria are shown in orange. Values on the branches represent bootstrap values based on 100 bootstrap replicates. The organismal tree is based on a 10 locus multi-locus sequence analysis as described in Den Bakker et al. . The topology of this tree is congruent with a tree based on the MLST scheme used in Ragon et al. .
To determine if the phylogeny of O-antigen cluster genes is discordant with the organismal phylogeny across the entire Listeria genus we analyzed the gene content and synteny for both clusters in non-L. monocytogenes Listeria species for which genome sequences are available. In addition, we investigated other genes outside the two clusters which displayed a serotype related phylogenetic pattern, genes that were uniquely found within one serotype or the other, and genes that had been implicated in L. monocytogenes O-antigen variation in previous publications , ,  (see supplemental Table S1 for key results). To aid in the analysis we also serotyped five additional Listeria strains (see Table 1). Gene content and gene order in cluster 1 was found to be highly similar between serotypes 1/2 (found in L. monocytogenes and L. seeligeri), 3 and 7 (found in L. seeligeri FSL N1-067 and in L. monocytogenes ), irrespective of the species in which the cluster was found (Fig. S1). While gene content and gene order in cluster 1 in serotypes 1/2, 3 and 7 are extremely similar among L. monocytogenes strains and even between species (L. seeligeri versus L. monocytogenes), we found this cluster to display subtle differences when serotypes 4, 5 and 6 were compared. Cluster 1 in L. innocua CLIP 11262 (serotype 6a) was found to be identical in gene content and gene order to L. monocytogenes serotype 4b and L. monocytogenes FSL J1-208 (serotype 4a). Gene content and gene order in cluster 1 of L. welshimeri SLCC5334 serotype 6b was found to be identical to L. monocytogenes serotype 4a (strain HCC23) and serotype 4c (strain FSL J2-071). We further found homologs of gltA and gltB in cluster 1 in L. innocua FSL J1-023 serotype 4b and in L. ivanovii serotype 5 (see Fig. S1). The gltA-gltB gene cassette was previously reported to be serotype 4b specific and involved in wall teichoic acid glycosylation . This gene cassette is found in a region approximately 1.6 Mb removed from cluster 1 in L. monocytogenes serotype 4b isolates such as F2365 (LMOf2365_2740 and LMOf2365_2741).
To further probe the evolution of the two O-antigen clusters, we constructed gene phylogenies for genes, within these clusters, that had orthologs in both serotypes 1/2 and 4. Two phylogenetic patterns could be found among the shared genes in O-antigen cluster 1 (Fig. 4B): (i) a serotype-specific pattern, showing a clade consisting of serotypes 1/2, 3 and 7 and a clade consisting of serotypes 4, 5, and 6, (Fig. 4B, orange pattern; seven genes), and (ii) a pattern mirroring the organismal phylogeny of Listeria (Fig. 4B, blue pattern). The shared genes in cluster 2 also showed two distinct phylogenetic patterns (Fig. 4C): (i) a phylogenetic pattern reminiscent of the organismal phylogeny of Listeria and similar to that seen in cluster 1 (Fig. 4C, blue pattern), and (ii) a serotype-associated pattern for L. monocytogenes, L. innocua, L. welshimeri and L. marthii, but a non-serotype specific pattern for L. seeligeri and L. ivanovii (Fig. 4C, orange pattern; three genes). Cluster 1 genes with a serotype specific phylogenetic pattern were tagG (LMOf2365_1091) and tagH (LMOf2365_1092), an UTP-glucose-1-phosphate uridylyltransferase (homologous to rfbA: LMOf2365_1099), a glycosyl transferase (LMOf2365_1100), ribitol-5-phosphate cytidylyltransferase (LMOf2365_1101), tagB (CDP-glycerol:N-acetyl-beta-D-mannosaminyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenylglycerophosphotransferase: LMOf2365_1104) and a putative sorbitol dehydrogenase (LMOf2365_1105). Shared genes with a serotype specific phylogenetic pattern in cluster 2 were an autolysin (LMOf2365_2530), a gene annotated as UDP-N-acetylglucosamine 1-carboxyvinyltransferase (LMOf2365_2524), a transcription termination factor (LMOf2365_2523), and the cell wall teichoic acid glycosylation protein GtcA (LMOf2365_2522). Most of these shared genes with a serotype-associated phylogenetic pattern are homologous to genes implicated in basic functions in wall teichoic acid synthesis in other Firmicutes , , and in L. monocytogenes , , . All wall teichoic acid associated genes that display a serotype-associated phylogenetic pattern show a high nucleotide divergence (e.g., 8.2–40%) between homologous genes of lineage I L. monocytogenes serotype 4b and L. monocytogenes 1/2b strains, while the nucleotide divergence between L. monocytogenes 1/2a (lineage II) and L. monocytogenes 1/2b (lineage I) strains is between 1.0 and 2.7%. The high nucleotide divergence suggests that 1/2- and 4- like serotypes predate the most common ancestor of L. monocytogenes. The fact that L. monocytogenes lineage III and IV, and closely related species such as L. marthii and L. innocua display 4 and 6 like serotypes, suggests that the most recent common ancestor of L. monocytogenes putatively was of serotype 4, and the 1/2-like serotypes were introduced, through horizontal gene transfer, in the ancestral population of L. monocytogenes lineage I and II. Alternatively both 1/2-like and 4-like serotypes could have been present in the ancestral L. monocytogenes population, and 4-like serotypes were subsequently lost in lineage II.
To reconstruct the putative evolutionary history of serotypes in L. monocytogenes we reconciled the gene trees with serotype-specific patterns (Fig. 4B, red and orange patterns) with the organismal tree of the genus Listeria (similar to Fig. 4B, blue pattern) using the AnGST  and Mowgli  algorithms. Both algorithms simultaneously account for gene loss, gene duplications and horizontal gene transfer. The majority of the reconciliations for both cluster 1 genes (6/7 genes) and cluster 2 genes (3/3 genes) support a scenario in which horizontal gene transfer was responsible for the introduction of the 1/2 serotypes in the ancestral population of L. monocytogenes lineage I and II (Fig. 5). In the case of cluster 1, the putative donor of the genes encoding expression of the L. monocytogenes 1/2 serotypes was the ancestral population of L. seeligeri. Reconciliations of the cluster 2 genes suggest that the 1/2 serotypes arose once, either in the ancestral populations of L. welshimeri or L. seeligeri. The gene cluster was then transferred from these populations into the ancestral population of L. monocytogenes lineage I and II, and were subsequently lost in the donor populations.
This construction suggests that serotype 1/2 genes were horizontally transferred from L. seeligeri to an ancestor of L. monocytogenes lineages I and II. The origin of the serotype 1/2 cluster is unclear, we hypothesize that this cluster putatively originated in the most recent common ancestor of the L. seeligeri and L. ivanovii clade (as indicated by the dashed line). Serotype 4 genes appear to be largely inherited by vertical descent, except for a lateral transfer of genes from L. welshimeri into some strains of L. monocytogenes lineage III (dotted red line).
In contrast to genes of the serotype 1/2 gene clusters, the serotype 4 O-antigen clusters followed a largely vertical descent through Listeria species (Fig. 5). The one exception to this mode of inheritance appears to be a replacement, in lineage III serotype 4a and 4c strains, of part of the ancestral O-antigen cluster 1 with a L. welshimeri type O-antigen cluster 1 through horizontal transfer. Horizontal transfer of the O-antigen cluster 1 into lineage III serotype 4a and 4c strains is further supported by the similarity in synteny of this cluster in both donor (L. welshimeri SLCC5334) and recipient (L. monocytogenes lineage III serotype 4a and 4c; see Fig. S1). All gene tree reconciliations support a most recent common ancestor of L. monocytogenes, which had serotype 4.
The phylogenetic patterns detailed above suggest the occurrence of homologous recombination within cluster 2 between L. monocytogenes donors and recipients. To test for homologous recombination and sequence tracts involved in these recombination events we used RAT  to detect putative breakpoints. We subjected sequences representing the entire cluster 2 (minus large indel regions) of L. monocytogenes serotypes 1/2b, lineage I 4b, and 1/2a to this analysis. The results of this analysis suggest that two sequence tracts within cluster 2 were putatively introduced into the lineage I serotype 1/2b strains from a lineage II serotype 1/2a donor. These tracts include (i) a tract encoding part of a homoserine dehydrogenase (lmo2547), the entire 50S ribosomal protein L31, gtcA, transcription termination factor Rho, UDP-N-acetylglucosamine 1-carboxyvinyltransferase, a hypothetical protein (lmo2555 homolog) and a glycosyl transferase, and (ii) a tract encoding an autolysin.
L. monocytogenes genomes are highly conserved and free of major genomic rearrangements even when compared to closely related Listeria species . However, our work here suggests that this picture does not fully represent what appears to be an unappreciated property of this species; the Listerial genomes show evidence for uneven vulnerability to the gain of, or tolerance for, horizontal transfer based on position in the genome. The first 65° of the chromosome is enriched for accessory genes, while the last 295° is enriched for core genes; this genome compartmentalization is absent from the closely related bacteria such as S. aureus and B. cereus. There could be an adaptive value in such a behavior although the molecular mechanism responsible for this is unresolved. We also find a series of genes, which cluster phylogenetically according to serotype, but not according to the organismal phylogeny. The majority of these genes is organized in two gene clusters, and reconstruction of the putative evolutionary history of these clusters shows these genes have a complex evolutionary history, involving multiple instances of horizontal gene transfer.
The enrichment of the first 65° degrees of the genome for accessory genes can only be partly attributed to the eight hotspots recently described by Kuenne et al.  for this chromosomal region, as less than 25% of the accessory genome could be attributed to these hotspots. Overall, we found 38% of the accessory genome (prophage related genes not included) in the first 65° degrees of the genome. Kuenne et al.  used a strict definition of an insertion deletion hotspot (‘hotspots were defined by the localization of at least three non-homologous insertions between mutually conserved core genes’). We find that a large part of the accessory genome found in the first 65° degrees is found outside of the eight hotspots identified previously  and in the work reported here. We thus propose that this portion of the chromosome may be more accurately described as a "hot region" for the gain of horizontally acquired information.
The genome partitions we find in L. monocytogenes appear to stem from differences in selective pressures and different rates of gene insertion. The former is supported by the finding in L. monocytogenes genomes that core genes in the first 65° of the genome are under less purifying selection than genes in the last 295°, indicating that to some extent, the position of a gene within the genome may affect its rate of evolution regardless of whether the gene is part of the core or accessory genome. The size of intergenic regions is thought to be driven by, and reflective of, the balance between insertions and deletions . The longer intergenic distances in accessory-rich region of the genome may reflect the dynamic nature of this region where the balance is tipped toward insertions of new accessory operons.
What molecular mechanism could account for one region becoming more prone to the accretion of foreign DNA? One possible explanation could involve systems that physically sequester regions of the genome. For example in E. coli the terminus region is physically and functionally gathered together through the action of the MatP protein that recognizes a series of sites (matS) in this region of the chromosome . This region containing matS sites is constrained by another protein that seems to allow the terminus region to interact with the division machinery ). If a similar system worked in the first 65 degrees of the L. monocytogenes chromosome it could conceivable render this region differentially accessible for new DNA sequences that enter the cell. Interestingly the terminus region of the E. coli chromosome appears to evolve differently from the rest of the genome displaying lower rates of recombination without higher mutation rates .
Alternatively, as suggested previously for E. coli , one could also imagine a series of "domino" effects that follow the acquisition of a very large segment of DNA. If beneficial gene products were encoded in this DNA segment it could encourage maintenance of the new large DNA segment. However, genes on this same stretch of DNA that were under negative selection or were neutral would allow (if not encourage) the acquisition of more insertions. This entire new region would then be active for gain and loss of genes for a protracted period of time as deletions also occurred across the regions under negative or neutral selection. Eventually the original genes that allowed the new DNA to become fixed in the population would be unrecognizable from other core genes from the species, but the process of gaining more genetic information in the region and winnowing of the sequences under negative and neutral selection could occur over a much longer period of time. The net result would be a mosaic of core and accessory genes without any necessary association to mobile elements. Interestingly only one complete prophage can be found in the first 65° of the chromosome. Core genes found in this region may have only relatively recently become fixed in the population (or part of the core genome), which may explain why this region is more rapidly evolving compared to the rest of the chromosome.
Regardless of the mechanism that accounts for the regional effect suggested by our analyses, the compartmentalization of the Listeria chromosome into accessory gene rich and poor regions could provide an evolutionary risk management strategy analogous to one recently described in E. coli, where the chromosome is divided into mutational hot and cold spots . In E. coli, mutational cold spots (regions with a lower mutation rate) coincide with highly expressed genes and genes under strong purifying selection, thereby reducing the risk of deleterious mutations in these regions .
Functional enrichment of transcriptional regulators, cell surface genes, and phosphotransferase systems in the accessory genome highlights the selective pressures faced by contemporary strains of L. monocytogenes. The complex regulation potentially required for networks of auxiliary or core genes to respond to these pressures may explain the abundance of transcription factors among the auxiliary genome. Enrichment of cell surface-related genes in the accessory genome of suggests that there is sustained selective pressure on L. monocytogenes to continually remodel the cell surface, playing a putative role in host specificity, host interactions, and the evasion of predators such as bacteriophages and protists in the non-host environment. Enrichment of cell surface-related genes in L. monocytogenes was also found in previous array based studies , . These cell wall-enriched accessory genes include internalins, a class of genes that also encodes well characterized virulence factors such as internalin A, internalin B and internalin C . The finding that phosphotransferase systems are enriched in the auxiliary genome suggests a selective pressure for L. monocytogenes to maintain a diverse repertoire of sugar transporters to cope with the diverse carbon sources in both hosts and the environment . Another explanation for the diversification of phosphotransferase systems could involve interaction with other microbes, as it has been shown that certain phosphotransferase systems in L. monocytogenes are putative targets for bacteriocins . A high diversity of phosphotransferase systems, combined with functional redundancy, may be a way to reduce bacteriocin sensitivity within host microbial communities.
While most genes in the L. monocytogenes genome follow a pattern of vertical descent, O-antigen associated genes and gene clusters seem to have distinct phylogenetic histories suggesting lateral transfers. A gene-by-gene gene-tree reconciliation approach suggests lateral transfer of O-antigen cluster 1 from a serotype 1/2 or 7 L. seeligeri ancestor into the serotype 4 L. monocytogenes ancestor. A putative change of function of O-antigen associated genes in cluster 2 in the L. seeligeri donor could explain the discrepancy between the phylogenetic patterns of cluster 1 and cluster 2 genes, where cluster 1 genes show a serotype specific pattern across Listeria species and cluster 2 genes only show a serotype-specific pattern within L monocytogenes. The fact that O-antigen cluster 2 genes in L. seeligeri 1/2b or 7 do not phylogenetically cluster according to serotype, suggests that genes in O-antigen cluster 1 are probably the most important determinants of O-antigen serotype. A break point analysis of L. monocytogenes cluster 2 suggests that Lineage I 1/2b serotype strains only recently acquired the serotype 1/2 gene fragments from Lineage II 1/2a donors. Further experimental work will be needed to clarify the role of cluster 1 and cluster 2 genes in serotype expression in different L. monocytogenes and Listeria species serotypes.
While serotype 1/2 was previously hypothesized to be the ancestral serotype in L. monocytogenes , our data support the alternative hypothesis, proposed here for the first time that 4-like serotypes were present in the ancestral population of L. monocytogenes lineages. This hypothesis seems to be supported by the observation that both lineage III and IV display 4-like serotypes, while the species most closely related to L. monocytogenes (i.e., L. innocua and L. marthii) also have 4-like serotypes. Based on the current data it is hard to refute the possibility that genes encoding serotype 1/2 expression (i.e., the clusters associated with this O-antigens) were introduced in the ancestor of both lineages I and II, and subsequently replaced by serotype 4 genes in a subset of lineage I. Additionally, while our gene tree reconciliations suggest that L. seeligeri was a donor of clusters 1/2, the reverse transfer cannot be excluded at this stage. More research on the function and evolution of these O-antigen related genes is necessary to unravel their complex evolutionary history and involvement in host-pathogen and bacteriophage interactions.
Comparison of O-antigen cluster 1 in L. monocytogenes and closely related Listeria species.
Comparison of O-antigen cluster 2 in L. monocytogenes and closely related Listeria species.
Summary of phylogenetic patterns found for wall teichoic and lipoteichoic acid associated genes in Listeria.
We acknowledge the contribution of the Broad Institute Genome Sequencing Platform. We would like to thank Dr. Zuzana Kecerova and Dr. Cheryl L. Tarr from the Centers for Disease Control and Prevention, Atlanta GA for serotyping L. innocua, L. marthii and L. seeligeri isolates. We thank Renato H. Orsi and Jennifer Wortman for their helpful comments on the manuscript.
Conceived and designed the experiments: HCdB CAD BJH BWB MW. Performed the experiments: SKY TAH QZ CDK CY. Analyzed the data: HCdB CAD ADG JEP. Wrote the paper: HCdB CAD BJH JEP MW.
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