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Diet Segregation between Cohabiting Builder and Inquiline Termite Species

  • Daniela Faria Florencio,

    Affiliations Departamento de Entomologia, Lab Termitologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil, Departamento de Bioquímica, Universidade Federal de Santa Catarina, Curitibanos, Santa Catarina, Brazil

  • Alessandra Marins,

    Affiliations Departamento de Entomologia, Lab Termitologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil, Department of Biology, Pennsylvania State University, University Park, Pennsylvania, United States of America

  • Cassiano Sousa Rosa,

    Affiliations Departamento de Entomologia, Lab Termitologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil, Faculdade de Engenharia, Universidade do Estado de Minas Gerais, João Monlevade, Minas Gerais, Brazil

  • Paulo Fellipe Cristaldo,

    Affiliations Departamento de Entomologia, Lab Termitologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil, Infochemicals Research Team, Institute of Organic Chemistry and Biochemistry, Prague, Czech Republic

  • Ana Paula Albano Araújo,

    Affiliation Núcleo de Ecologia, Universidade Federal de Sergipe, São Cristóvão, Sergipe, Brazil

  • Ivo Ribeiro Silva,

    Affiliation Departamento de Solos, Laboratório de Isótopos Estáveis, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil

  • Og DeSouza

    og.souza@ufv.br

    Affiliation Departamento de Entomologia, Lab Termitologia, Universidade Federal de Viçosa, Viçosa, Minas Gerais, Brazil

Diet Segregation between Cohabiting Builder and Inquiline Termite Species

  • Daniela Faria Florencio, 
  • Alessandra Marins, 
  • Cassiano Sousa Rosa, 
  • Paulo Fellipe Cristaldo, 
  • Ana Paula Albano Araújo, 
  • Ivo Ribeiro Silva, 
  • Og DeSouza
PLOS
x

Abstract

How do termite inquilines manage to cohabit termitaria along with the termite builder species? With this in mind, we analysed one of the several strategies that inquilines could use to circumvent conflicts with their hosts, namely, the use of distinct diets. We inspected overlapping patterns for the diets of several cohabiting Neotropical termite species, as inferred from carbon and nitrogen isotopic signatures for termite individuals. Cohabitant communities from distinct termitaria presented overlapping diet spaces, indicating that they exploited similar diets at the regional scale. When such communities were split into their components, full diet segregation could be observed between builders and inquilines, at regional (environment-wide) and local (termitarium) scales. Additionally, diet segregation among inquilines themselves was also observed in the vast majority of inspected termitaria. Inquiline species distribution among termitaria was not random. Environmental-wide diet similarity, coupled with local diet segregation and deterministic inquiline distribution, could denounce interactions for feeding resources. However, inquilines and builders not sharing the same termitarium, and thus not subject to potential conflicts, still exhibited distinct diets. Moreover, the areas of the builder’s diet space and that of its inquilines did not correlate negatively. Accordingly, the diet areas of builders which hosted inquilines were in average as large as the areas of builders hosting no inquilines. Such results indicate the possibility that dietary partitioning by these cohabiting termites was not majorly driven by current interactive constraints. Rather, it seems to be a result of traits previously fixed in the evolutionary past of cohabitants.

Introduction

An efficient strategy for organisms which depend on nesting is to inhabit the nest of another species, because this avoids building costs while keeping the benefits of such structures. It is not surprising, therefore, that many inquiline species are spread throughout virtually all groups of animals. An intriguing issue is how invaders deal with potential conflicts with the builder, especially if invaders and builders cohabit, as frequently occurring with termite inquilines and their termite hosts. Here we provide evidence that inquilinism in certain termite nests seems to be eased by the use of conflict-avoiding strategies on the part of inquilines.

Examples of nest invaders include, but are not restricted to, nest-usurping woodpeckers, cuckoos and cowbirds [1], [2], joint nesting salamanders [3], inquiline bumblebees [4], and social parasitic butterflies [5]. In termite nests, intruders range from vertebrates such as birds [6] and bats [7] to a wide variety of arthropods [8], [9]. Most commonly, these assemblages are composed of a termite species that builds and maintains the nest, plus entire invertebrate food webs [10], [11] whose members are generally referred to as termitophiles. A particular subset of these is formed by termites that inhabit termite nests and may contribute to either nest maintenance or nest decay [12], the so called inquilines [13][17].

Inquiline termites form a particular group of invaders because, as their hosts, inquilines are detritivores. Risks imposed by inquiline termites are therefore rather distinct from those imposed, for instance, by predatory cohabitants such as larvae of elaterid beetles in termitaria [18], or the larvae of Microdon flies [19] and Lycaenidae butterflies [5] in ant nests. The absence of predation risks by no means implies the absence of trouble to the builder: negative interactions are still bound to arise if inquilines, e.g., feed on stored products or on the lining of the nest walls to a degree that requires constant replenishment or repair by the builders. At the very least, contests could be triggered when inquilines use a space originally built for the builder’s nestmates.

Dealing with such conflicts so that they represent bearable costs to the builder is key to the stability of cohabitation over ecological and evolutionary time. Therefore, a plausible hypothesis is that inquiline selection favours the adoption of strategies to minimise costs to the builder, which can be achieved by inflicting low loss or offsetting losses with an associated benefit. A wide range of strategies fulfil such aims, among which segregation of feeding resources is an obvious example of conflict avoidance. A possibility that cannot be excluded is that inquilinism is based on non-interactive processes: opportunistic inquilines occupy abandoned parts of termitaria and remain there unnoticed by the builders. In this case, the relationship could be evolutionarily stable because the use of such spaces would not be deleterious to the builder but would enhance the inquilines’ fitness through reduction of their own building costs.

In the present study we analysed the coexistence of termite builders and inquiline species in the same termitarium, in the field, with a focus on one of the mechanisms that could explain this interaction: the diet use by the species involved. To this end we evaluated diet coincidence between two builder termite species and 12 associated inquiline species, inspecting the stable isotopic signature of individuals from 14 termite nests in a savannoid ecosystem (cerrado) in South-eastern Brazil. Our rationale was that the diet of inquilines should differ from that of builders and the difference can be inferred from distinct / and / ratios for the termites. As a null hypothesis we consider that if invasion of these termitaria occurred merely by chance, without any evidence of past or present interaction, we would not find any consistent diet pattern for builder and inquiline species. In short, we argue here that one of the reasons for the coexistence of these builders and inquilines is that diet segregation minimises negative interactions and favours cohabitation in the same termitarium.

Materials and Methods

Ethics Statment

All necessary permits were obtained for the described study, which complied with all relevant regulations of Brazil. This includes collecting and transportation permits from IBAMA (The Brazilian Institute for the Environment and Renewable Natural Resources), permission from EMBRAPA (The Brazilian Enterprise for Agricultural Research) to conduct the study on their site, as well as tacit approval from the Brazilian Federal Government implied by granting the authors the post of Scientific Researcher.

Definition of Terms

The term “termitarium” is used here to denote the physical epigeic structure built by termites (for taxonomic status see [20], [21]). We use “mound” and “nest” as synonyms of termitarium. “Colony” denotes the assemblage of individuals of a given species living and cooperating within the nest. “Coexistence” and “cohabitation” are used as synonyms and refer to the simultaneous occurrence of colonies of different termite species within a given termitarium, without implication of reciprocal positive or negative influences.

Diets exploited by termites were inferred from concentrations of stable carbon and nitrogen isotopes in termite bodies obtained by measuring / and / ratios. Termites from the same colony may forage on distinct lignocellulose sources with distinct degrees of decomposition. Therefore, the diet of a termite colony is characterized here by a set of / and / pairs obtained from several individuals from the same colony, this set circumscribing a bidimensional space in a Cartesian plot whose axes represent the concentration of such isotopes in termite bodies.

Study Site

The study was carried out in the Brazilian cerrado, an environment physiognomically but not floristically similar to a savannah, near the town of Sete Lagoas (27′ S, 14′ W, altitude 800–900 m above sea level), Minas Gerais State, South-eastern Brazil. In Köppen’s classification, the study area has an Aw climate (equatorial with dry winter) [22]. The total precipitation in 2008 was 1607 mm and the mean monthly temperature ranged from to [23]. Fire often occurs naturally in the cerrado and the termites [24] and other organisms [25] that live there tolerate fire or depend on it to survive. Epigeous termitaria are a common feature of such an environment and inquilines frequently inhabit these termite mounds [26].

Sampling

We sampled, from 24 to 28 July 2008 (7∶30–16∶00 h), 14 termitaria whose builder colonies were still alive and (apparently) healthy. These termitaria showed no sign of damage, were epigeic, and were easily removed from the soil without breaking its hypogeic portion. The termite builder species studied, Velocitermes heteropterus and Constrictotermes cyphergaster (both Termitidae: Nasutitermitinae), do not normally build termitaria presenting a significant hypogeic portion. It is worth noting that C. cyphergaster, which typically builds arboreal nests, can also build epigeous ones [27]. The termitaria were removed from the field, put into plastic bags, labelled, and taken to the laboratory. The vegetation and landscape were similar around all the termitaria sampled.

Once in the lab, the entire termitaria were carefully inspected to extract individuals using soft entomological forceps. Individuals from the same species grouped together were considered as belonging to the same cohabitant colony. Duplicate samples were taken from these cohabitant colonies, one for taxonomic identification and the other for isotopic analyses.

Specimens used for identification were preserved in 80% alcohol, labelled, and subsequently identified to species (or morpho-species) level according to Mathews [12] and literature referred to by Constantino [28]. Identifications were confirmed by comparison with the termite collection of the Entomological Museum of the Federal University of Viçosa (MEUV), where voucher specimens were deposited.

The builder species of each termitarium was determined by matching the termitarium physical traits with previous published accounts [12], [29] regarding size, geometric form, composition (soil or carton), wall texture, and wall hardness. In addition, builders tend to be far more abundant inside their termitarium than any inquiline.

Inquilines were identified as species whose colonies presented individuals of distinct instars, indicating that reproductive pairs were active and the colony was integrated in the environment. Some inquiline colonies were not populous enough to supply a minimum biomass of workers for isotopic analyses so their diet patterns were not mapped (these are denoted by ‘o’ for others in Table 1).

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Table 1. Termite (morpho)species cohabiting termitaria in a ‘cerrado’ ecosystem.

https://doi.org/10.1371/journal.pone.0066535.t001

Stable Isotope Analysis

We used stable isotope concentrations to infer diet because the isotopic composition of the body of an animal reflects the food consumed and assimilated during its lifetime [30], [31]. Within a given environment, comparatively higher values indicate a termite diet biased towards more humified organic matter, whereas lower values point to a less decomposed, even xylophagous diet. Bourguignon et. al. [32] presented a practical example of such a classification.

Termite workers of each species in the termitaria were sorted, when possible, into 10 subsamples, each with a sufficient number of individuals to obtain a dry biomass of 1.5 g for full-body isotopic analysis. Colonies meeting this criterion are denoted by ‘b’ (for builders) and ‘i’ (for inquilines) in Table 1. We used only workers for stable isotope analysis, not only because these are the most abundant individuals in a termite colony but also because they forage and feed other castes in the colony [33]. This procedure also eliminated any possible intercaste effects on isotopic values [34].

Each subsample was placed in a vial with distilled water and was immediately frozen until the analyses could be performed. Water was removed by freeze-drying for approximately 48 h to dehydrate the termites, prevent decomposition and maintain the original / and / ratios. The subsamples were then ground with a mortar and pestle and sieved through a 100-mesh sieve. Carbon and nitrogen isotope ratios were measured for each subsample independently, using an isotope ratio mass spectrometer (IRMS, ANCA-GSL 20–20, SerCon, UK) in the Laboratory of Stable Isotopes, Soils Department, Federal University of Viçosa (UFV). The analytical precision was estimated to be 0.1‰ for carbon and 0.2‰ for nitrogen. The natural abundance of and nitrogen is expressed as per thousand (‰) deviation from an international standard(belemnite of the Pee Dee Formation in South Carolina, USA (PDB) for carbon and atmospheric nitrogen (air) for nitrogen). The ratios of the heavy ( or ) to the light isotope ( or ), typically corresponding to rare and abundant isotopes are hereafter referred to as “isotopic ratios” [35] and are referenced by and .

Data Analysis

Diet limits were statistically defined as Bayesian standard ellipses plotted around pairs of and points representative of termites’ diet space, such ellipses being to bivariate data as standard deviation is to univariate data. Because these ellipses define the statistical limits for the dimensions of each diet, overlapping ellipses indicate statistically indistinguishable diet spaces. Ellipses and associated metrics were calculated using siber routines [36] from siar package [37], under R statistical computing environment [38].

Ellipses were estimated according to three distinct and complementary views of the dataset. Initially, a single ellipsis was estimated for the whole community of cohabitants within a given termitarium, thereby allowing comparisons among whole termitaria across the sampled region. Overlapping ellipses would indicate similarity between diets among termitaria in spite of their spatial distribution over the sampled region. Then, the data relative to the full set of inquilines of a given builder species were amassed (across all termitaria) in a single ellipsis, thereby allowing comparisons with the single ellipsis of the respective builder species, also amassed across all termitaria. This allowed to infer general patterns for diet spaces of inquilines versus builders. Finally, individual ellipses were plotted for each cohabitant within each termitarium, thereby allowing diet comparisons between builders and their respective inquilines within a given termitarium.

To infer on interactive processes regulating diet segregation we checked for correlation between the dimensions of diet spaces of cohabitants within each termitarium. If inquilines dynamically expand their diets at the expense of their host’s diets (or vice-versa), the dimension of their respective diet spaces across all termitaria should correlate negatively. Accordingly, diet spaces of builders living alone should be larger than those of builders cohabiting with inquilines. Analyses were carried out using Generalized Linear Modelling under normal errors followed by residual analyses to confirm the model suitability and the choice of error distribution. Initially, a subset of the data containing only termitaria having both, builders and inquilines, was subjected to a model in which the area of the builder's ellipsis (y-var) was correlated with the respective area of the ellipsis formed by their respective inquilines taken together (x-var). The identity of the builder entered the model as a covariate, both as a single term and as part of the first order interaction. Another independent model compared the average area of the builder's ellipsis (y-var) between termitaria with and without inquilines (x-var). This was only possible on termitaria built by C. cyphergaster because for those both instances of the x-variable were available. Models were simplified by deleting non-significant terms () from the initial model according to their complexity, starting with the most complex term, following recommendations by Crawley [39].

Results

Species Distribution among Termitaria

A survey carried out in the study area revealed that termitaria were 4.4 1.7 m (mean SD) apart from their four nearest neighbouring termitaria. This survey included, but was not restricted to, the termitaria studied here.

Some 20 species of termite inquilines were found in the termitaria, of which 12 species presented individuals enough to be analysed isotopically. A total of 13 species occurred only once (Table 1) and seven occurred in two or more termitaria. Termitaria sheltered between zero and six inquiline species. Termitaria of V. heteropterus sheltered between one and six inquiline species at once, whereas termitaria of C. cyphergaster housed between zero and one inquiline species.

Heterotermes longiceps was the sole inquiline species found in termitaria of both builder species, but it was neither frequent nor abundant: only two very small colonies were recorded, the largest of which comprised approximately 40 individuals. The remaining 19 inquiline species were not shared between builder species, suggesting species-specific differences in the ability to coexist with other species. Supporting such a trend, Inquilinitermes microcerus did not occur in termitaria of V. heteropterus but it was found only in termitaria of C. cyphergaster. This is in line with previous reports that I. microcerus is an obligatory inquiline of C. cyphergaster [12].

The 14 termitaria studied housed 29 inquiline colonies along with the builder colony (‘i’+‘o’ in Table 1), of which 18 colonies presented individuals enough to be analysed isotopically (‘i’ in Table 1). Termitaria housing multiple colonies showed no evidence of more than a single colony of a given cohabitant species.

Diet Segregation

Termitaria overlapped each other regarding the overall diet space of their community of cohabitants (Fig. 1) indicating that, in average, communities exploited rather similar diets despite being confined to distinct termitaria. A single C. cyphergaster nest did not overlap the others (Fig. 1, leftmost ellipsis, corresponding to nest c7). This nest is devoid of inquilines and its detachment was not strong enough to scramble the statistical non-overlaping trend presented by the other nests, as it is shown in Fig. 2, “builder alone”. The diets of builder and inquiline species never overlapped (Fig. 2, 3 and 4). This diet segregation tended to be majorly driven by isotopic dimension, with inquilines occupying a higher trophic position than builders (Fig. 2), albeit still within the detritivore level (horizontal dotted lines in all figures denote changes in trophic position, as it is generally agreed that trophically distinct organisms would differ by 3‰ in [40]).

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Figure 1. Overall summary of diet spaces of termite communities cohabiting termitaria, as inferred from their isotopic profile.

Each panel depicts a set of termitaria of a given builder (Constrictotermes cyphergaster or Velocitermes heteropterus). Axes represent the concentration of stable carbon and nitrogen isotopes in termite bodies. Each dot refers to a group of termite workers weighing 1.5 g. Each Bayesian standard ellipsis represents a single termitarium. Dotted lines parallel to x-axis define 3‰ intervals thought to correspond to trophically distinct positions. Both, inquiline and builder species are included. See Table 1 for termite species identities.

https://doi.org/10.1371/journal.pone.0066535.g001

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Figure 2. Overall summary of diet spaces of builder and inquilines termites, as inferred from their isotopic profile.

Each panel depicts a set of termitaria of a given builder (Constrictotermes cyphergaster or Velocitermes heteropterus). Axes represent the concentration of stable carbon and nitrogen isotopes in termite bodies. Each dot refers to a group of termite workers weighing 1.5 g. Each Bayesian standard ellipsis represents the full set of builders or inquilines amassed across all termitaria studied. Dotted lines parallel to x-axis define 3‰ intervals thought to correspond to trophically distinct positions. See Table 1 for termite species identities.

https://doi.org/10.1371/journal.pone.0066535.g002

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Figure 3. Diet spaces of termite species coexisting in termitaria built by Velocitermes heteropterus, as inferred from their isotopic profile.

Each panel represents a single termitarium. Axes represent the concentration of stable carbon and nitrogen isotopes in termite bodies. Each dot refers to a group of termite workers weighing 1.5 g. Bayesian standard ellipses refer to the builder and its inquiline termite species. Dotted lines parallel to x-axis define 3‰ intervals thought to correspond to trophically distinct positions.

https://doi.org/10.1371/journal.pone.0066535.g003

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Figure 4. Diet spaces of termite species coexisting in termitaria built by Constrictotermes cyphergaster, as inferred from their isotopic profile.

Each panel represents a single termitarium. Axes represent the concentration of stable carbon and nitrogen isotopes in termite bodies. Each dot refers to a group of termite workers weighing 1.5 g. Bayesian standard ellipses refer to the builder and its inquiline termite species. Dotted lines parallel to x-axis define 3‰ intervals thought to correspond to trophically distinct positions.

https://doi.org/10.1371/journal.pone.0066535.g004

There was also a general trend for diet segregation among inquiline species within termitaria, with only a single case of overlap out of 14 nests (Fig. 3, v3). Diet segregation among inquilines was also most obvious in the dimension.

Diet Shrinkage

There was no correlation between the areas of the builder’s diet space and that of its inquilines (, ). Accordingly, the diet areas of C. cyphergaster builders hosting inquilines were in average as large as the areas of builders hosting no inquilines (, ). This seems to lend support to the notion that inquilines and builders do not interfere to each other in terms of their diet.

Discussion

Differentiation in resource use has been considered one of the main mechanisms that facilitates species coexistence (for a comprehensive historical account, see [41]) that includes communities of plants [42], [43], fish [44], and insects [45], [46]. In communities of termites, interactions with respect to food resources have been identified as an important regulating factor; examples include species assemblages from the African savannah [47], [48] and the South American tropical rainforest [32], [34].

While dietary shifts seem to affect the coexistence of termite species in environments delimited by permeable borders, patterns of interaction with respect to diet are virtually unknown for termite species assemblages circumscribed by discrete physical barriers (but see [49] for competing insular termite populations), especially those cohabiting the same termitarium.

Such spatially confined populations represent suitable scenarios for studying dietary shifts as determinants of species coexistence. Because barriers restrict spatial adjustments that could preclude species interactions, the importance of dietary adjustments may in turn be amplified. In fact, for the termite builder–inquiline assemblages studied here, feeding resource segregation appears to be typical, if not the determinant, of cohabitation in the same termitarium. Diet spaces for inquilines never overlapped host’s spaces at both, regional (i.e. the sampled environment) and local (termitarium) scales (Figs. 2, 3 and 4). Mechanisms behind this diet segregation could include (i) local differences in the suitability and availability of resources, including predation constraints [50], [51], so that each set of cohabitants in a termitarium has access to a particular diet; and (ii) local-scale interspecific tradeoffs [52] leading to diet partitioning along a trophic continuum within the termitarium.

The fact that inquiline-bearing termitaria presented strong overlap regarding the overall diets of their cohabiting communities (Fig. 1) seems to point out that cohabitants had access to similar resources, and that is reinforced by the close proximity of all nests (in average 4.4 m apart). Additionally, consistent patterns of non-overlapping diets between builders and inquilines and among inquilines across all termitaria (Figs. 2, 3 and 4) seem to weaken the hypothesis of local differences in resources in favour of the trade-off hypothesis, even though these are not necessarily mutually exclusive.

Segregation in resource use on its own does not imply species interactivity since species can be assembled by chance events [53]. However, as well as the consistent differences observed for their actual diets (Fig. 3 and 4), the cohabiting termites studied here did not seem to be assembled at random (Table 1). Rather, inquiline species of V. heteropterus did not seem to be able to live in termitaria of C. cyphergaster and vice versa. This is reinforced by the presence of the obligatory inquiline I. microcerus [12], [29], [54], which was only found in C. cyphergaster nests. In fact, ocupation of C. cyphergaster nests by I. microcerus is not believed to occur at random but to depend on host/nest features [16]. Thus, inquiline occupation in these termitaria is likely to be related to the intrinsic characteristics of the species involved rather than being a simple chance event.

Diet segregation under such a deterministic scenario could result from feeding resource competition but the absence of overlap between the overall diet spaces of inquiline and builders would challenge this idea, because even when not sharing the same termitarium and hence not subject to potential conflicts, the diet of inquiline species never overlapped that of host species (Fig. 2). Indeed, the mere fact that inquilines did exploit distinct diets makes it risky to advocate some link between the observed segregation and contemporary competitive interactions. It seems therefore that dietary partitioning by cohabitants was not majorly driven by interactive constraints, a hypothesis also supported by the absence of correlation between the diet space areas of builder and inquiline species within termitaria (; ), where interactions would be highly likely. This is further supported by the fact that the average diet areas of C. cyphergaster builders did not expand significantly in the absence of inquilines (, ). In other words, interspecific tradeoffs as a force driving termite inquilinism in this system would more likely to have occurred – if at all – in the evolutionary past rather than in the contemporary ecological time frame (the ‘ghost of competition past’ [55]). An alternative and perhaps more conservative view is that current inquiline species are descended from specialist lineages, and never conflicted with the dietary requirements of their hosts.

Despite being not able to distinguish between the hypotheses of past interspecific trade-off versus pre-adaptations favouring specialization, our data reinforce both hypotheses over a hypothesis focusing on current competition. Although still within the detritivore trophic level, inquiline and builder species never shared the same trophic position (Figs 3 and 4) and were sometimes as much as four full positions apart (taking each trophic step as 3‰ units in , as in de Visser et al. [10]). Since inquiline species are obviously not predators but detritivores, such disparate trophic positions may indicate that they in fact feed on materials far more decayed than those used by the builder species. These could include stored organic material, the hosts faeces and dead bodies, and the lining of the termitarium walls, which is also composed of faeces. Although still open to investigation, this assumption is in line – at least regarding I. microcerus– with previous reports by Noirot [56] and Mathews [12] and recent evidence by Bourguignon et al. [32].

Diet differences were also observed among most inquiline species cohabiting the same termitarium; those that actually differed being arranged in stepwise trophic positions. It is possible that a trophic chain was established, with one inquiline species feeding on the by-products of its host, another feeding on the excreta and remains of this inquiline, and so on. Alternatively, inquiline species could selectively feed on distinct parts of the nest, and thus would have distinct and inputs. Termites are indeed able to feed selectively in the field [51] and can select soil particles from distinct layers to build specific mound structures, which in turn exhibit distinct C and N contents [57] most likely with characteristic and values. This would explain not only the consistent differences observed between builder and inquiline species regarding dimensions of their diets, but also the fact that diets of inquilines, albeit still distinct, differed sometimes in a single dimension and sometimes in both. In other words, under this scenario, inquiline species and their host would differ less markedly in than in (Figs. 1, 3 and 4) because by feeding on specific parts of the nest, inquiline species have access to a subset of the carbon resources collated by their host along with selected soil particles that are trophically distinct because they are cemented with the host’s faeces. The possibility that a given inquiline species could also differ from the builder and other inquiline species by foraging for distinct food outside the nest [12] remains to be considered. All in all, this would only reinforce the diet segregation patterns observed here.

In summary, we found evidence that, at least for the system at hand, cohabitation of termite species in the same termitarium was related to diet segregation that did not seem to be majorly constrained by interspecific interactions for food. Rather, inquilines exploited diets not used by their host, thereby circumventing conflicts over use of feeding resources.

Acknowledgments

We thank J.M. Waquil from the Brazilian Enterprise for Agricultural Research (EMBRAPA) for logistic support, E.E.L. Borges and J.M. Ferreira from the Laboratory of Analysis of Forest Seeds at the Federal University of Viçosa (UFV) for freeze-drying of termite samples, R. Constantino for help with termite identification, and C. Sperber for inspiring discussion. J. Šobotník, T. Bourguignon, S. Elliot, R. Solar and two anonymous referees kindly reviewed versions of the manuscript providing invaluable insights. AM is grateful to Shea Lab members (PennState, US) for productive discussions during the preparation of the manuscript. All necessary permits were obtained for the described study, which complied with all relevant regulations of Brazil. All computational work was performed using free software (GNU-Linux/Debian and Ubuntu, LaTeX, Gimp, Kile, LibreOffice, RStudio, and R plus its packages siar, RRJ, lattice, among others). This is contribution no. 51 from the Termitology Lab at UFV, Brazil (http://www.isoptera.ufv.br).

Author Contributions

Conceived and designed the experiments: DFF ODS AM CSR PFC APAA. Performed the experiments: DFF APAA AM CSR PFC ODS IRS. Analyzed the data: ODS DFF. Contributed reagents/materials/analysis tools: IRS DFF ODS. Wrote the paper: ODS DFF AM PFC APAA CSR.

References

  1. 1. Davies NB (2000) Cuckoos, cowbirds and other cheats. T. and A. D. Poyser, London.
  2. 2. Kronland WJ (2007) Nest usurpation by red-headed woodpeckers in southeastern Montana. Wilson J Ornithol 119: 486–489.
  3. 3. Harris RN, Hames WW, Knight IT, Carreno CA, Vess TJ (1995) An experimental analysis of joint nesting in the salamander Hemidaetylium scutatum (Caudata: Plethodontidae): the effects of population density. Anim Behav 50: 1309–1316.
  4. 4. Hines HM, Cameron SA (2010) The phylogenetic position of the bumble bee inquiline Bombus inexspectatus and implications for the evolution of social parasitism. Insectes Soc 57: 379–383.
  5. 5. Pierce NE, Braby MF, Heath A, Lohman DJ, Mathew J, et al. (2002) The ecology and evolution of ant association in the Lycaenidae (Lepidoptera). Annu Rev Entomol 47: 733–771.
  6. 6. Brightsmith DJ (2000) Use of arboreal termitaria by nesting birds in the Peruvian Amazon. The Condor 102: 529–538.
  7. 7. Dechmann DKN, Kalko EKV, Kerth G (2004) Ecology of an exceptional roost: energetic benefits could explain why the bat Lophostoma silvicolum roosts in active termite nests. Evol Ecol Res 6: 1037–1050.
  8. 8. Kistner DH (1990) The integration of foreign insects into termite societies or why do termites tolerate foreign insects in their societies. Sociobiology 17: 191–215.
  9. 9. Grassé P (1986) Termitologia. Masson, volume III, chapter Termitophiles et termitophilie. 235–367.
  10. 10. De Visser SN, Freymann BP, Schnyder H (2008) Trophic interactions among invertebrates in ter mitaria in the African savanna: a stable isotope approach. Ecol Entomol 33: 758–764.
  11. 11. Costa DA, Carvalho RA, Lima Filho GF, Brandão D (2009) Inquilines and invertebrate fauna associated with termite nests of Cornitermes cumulans (Isoptera, Termitidae) in the Emas National Park, Mineiros, Goiás, Brazil. Sociobiology 53: 443–453.
  12. 12. Mathews AGA (1977) Studies on termites from the Mato Grosso State, Brazil. Rio de Janeiro: Academia Brasileira de Ciências, 267 p.
  13. 13. Kalshoven LGE (1954) On the change in occupation by termite colonies of mounds after conversion of a jungle area into rice-fields in Java. Insectes Soc 1: 319–323.
  14. 14. Bouillon A (1970) Termites of the Ethiopian Region. In: Krishna K, Wessner FM, editors, Biology of Termites, New York and London: Academic Press, volume 2: 153–280.
  15. 15. Redford KH (1984) The termitaria of Cornitermes cumulans (Isoptera, Termitidae) and their role in determining a potential keystone species. Biotropica 16: 112–119.
  16. 16. Cristaldo PF, Rosa CS, Florencio DF, Marins A, DeSouza O (2012) Termitarium volume as a determinant of invasion by obligatory termitophiles and inquilines in the nests of Constrictotermes cyphergaster (Termitidae: Nasutitermitinae). Insect Soc 59: 541–548.
  17. 17. Darlington JPEC (2012) Termites (Isoptera) as secondary occupants in mounds of Macrotermes michaelseni (Sjöstedt) in Kenya. Insectes Soc 59: 159–165.
  18. 18. Costa C, Vanin SA (2010) Coleoptera larval fauna associated with termite nests (Isoptera) with emphasis on the “bioluminescent termite nests” from Central Brazil. Psyche 2010: 1–12.
  19. 19. Schönrogge K, Gardner MG, Elmes GW, Napper EKV, Simcox DJ, et al. (2006) Host propagation permits extreme local adaptation in a social parasite of ants. Ecol Lett 9: 1032–1040.
  20. 20. Inward D, Beccaloni G, Eggleton P (2007) Death of an order: a comprehensive molecular phylo genetic study confirms that termites are eusocial cockroaches. Biological Letters 3: 331–335.
  21. 21. Lo N, Engel MS, Cameron S, Nalepa Ca, Tokuda G, et al. (2007) Save isoptera: a comment on inward et al. Biology letters 3: 562–3.
  22. 22. Kottek M, Grieser J, Beck C, Rudolf B, Rubel F (2006) World map of the Kopen-Geiger climate classification updated. Meteorol Z 15: 259–263.
  23. 23. Agritempo (2009) Sistema de Monitoramento Agrometerológico. Embrapa Informática Agropecuária. Available: http://www.agritempo.gov.br/agroclima/sumario Accessed: 2009 Jul 13.
  24. 24. DeSouza O, Albuquerque LB, Tonello VM, Pinto LP, Reis-Junior R (2003) Effects of fire on termite generic richness in a savanna-like ecosystem (`Cerrado’) of Central Brazil. Sociobiology 42: 639–649.
  25. 25. Coutinho LM (1990) Fire in the ecology of the Brazilian cerrado. In: Goldammer JG, editor, Fire in the tropical biota: Ecosystem processes and global challenges, Berlin: Springer-Verlag, volume 84: 82–105.
  26. 26. Domingos DJ, Gontijo TA (1996) Multi-occupation of termite mounds in cerrado vegetation in south-eastern Brazil. Rev Bras Biol 56: 717–723.
  27. 27. Vasconcellos A, Araújo VFP, Moura FMS, Bandeira AG (2007) Biomass and population structure of Constrictotermes cypergaster (Silvestri) (Isoptera: Termitidae) in the dry forest of Caatinga, northeastern Brazil. Neotrop entomol 36: 693–698.
  28. 28. Constantino R (2002) An illustrated key to Neotropical termite genera (Insecta: Isoptera) based primarily on soldiers. Zootaxa 67: 1–40.
  29. 29. Araujo RL (1970) Termites of the Neotropical Region. In: Krishna K, Weessner FM, editors, Biology of Termites, New York and London: Academic Press, volume 2: 527–576.
  30. 30. DeNiro MJ, Epstein S (1978) Inuence of diet on the distribution of carbon isotopes in animals. Geochim Cosmochim Acta 42: 495–506.
  31. 31. Eggers T, Jones TH (2000) You are what you eat…or are you? Trends Ecol Evol 15: 265–266.
  32. 32. Bourguignon T, Šobotník J, Lepoint G, Martin JM, Hardy OJ, et al. (2011) Feeding ecology and phylogenetic structure of a complex neotropical termite assemblage, revealed by nitrogen stable isotope ratios. Ecol Entomol 36: 261–269.
  33. 33. Lee KE, Wood TG (1971) Termites and Soil. London and New York: Academic Press, 251 p.
  34. 34. Bourguignon T, Šobotník J, Lepoint G, Martin JM, Roisin Y (2009) Niche differentiation among Neotropical soldierless soil-feeding termites revealed by stable isotope ratios. Soil Biol Biochem 41: 2038–2043.
  35. 35. Fry B (2006) Stable isotope ecology. New York: Springer, 308 p.
  36. 36. Jackson AL, Inger R, Parnell AC, Bearhop S (2011) Comparing isotopic niche widths among and within communities: SIBER – Stable Isotope Bayesian Ellipses in R. J Anim Ecol. 80: 595–602.
  37. 37. Parnell AC, Inger R, Bearhop S, Jackson AL (2010) Source partitioning using stable isotopes: Coping with too much variation. PLoS ONE 5: e9672.
  38. 38. R Development Core Team (2012) R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria.
  39. 39. Crawley MJ (2007) The R Book. Chichester, West Sussex: John Wiley & Sons Ltd, 942 p.
  40. 40. Vander Zanden MJ, Shuter BJ, Lester NP, Rasmussen JB (2000) Within and among population variation in the trophic position of pelagic predator lake trout Salvelinus namaycush. Can J Fish Aquat Sci 57: 725–731.
  41. 41. Chase JM, Leibold MA (2003) Ecological Niches: linking classical and contemporary approaches. Chicago and London: The University of Chicago Press, 212 p.
  42. 42. Silvertown J (2004) Plant coexistence and the niche. Trends Ecol Evol 19: 605–611.
  43. 43. Fargione J, Tilman D (2005) Niche differences in phenology and rooting depth promote coexistence with a dominant C4 bunchgrass. Oecologia 143: 598–606.
  44. 44. Mason NWH, Lanoiselée C, Mouillot D, Wilson JB, Argillier C (2008) Does niche overlap control relative abundance in French lacustrine fish communities? A new method incorporating functional traits. J Anim Ecol 77: 661–669.
  45. 45. Sarty M, Abbott KL, Lester PJ (2007) Community level impacts of an ant invader and food mediated coexistence. Insect Soc 54: 166–173.
  46. 46. Tillberg CV, Holway DA, LeBrun EG, Suarez AV (2007) Trophic ecology of invasive Argentine ants in their native and introduced ranges. Proc Natl Acad Sci USA 104: 20856–20861.
  47. 47. Korb J, Linsenmair KE (2001) The causes of spatial patterning of mounds of a fungus-cultivating termite: results from nearest-neighbour analysis and ecological studies. Oecologia 127: 324–333.
  48. 48. Korb J, Linsenmair KE (2001) Resource availability and distribution patterns, indicators of com4 petition between Macrotermes bellicosus and other macro-detritivores in the Comoé National Park, Côte d’Ivoire. Afr J Ecol 39: 257–265.
  49. 49. Perdereau E, Dedeine F, Christidès JP, Dupont S, Bagnères AG (2011) Competition between invasive and indigenous species: an insular case study of subterranean termites. Biol Invasions 13: 1457–1470.
  50. 50. Korb J, Linsenmair KE (2002) Evaluation of predation risk in the collectively foraging termite Macrotermes bellicosus. Insectes Soc 49: 264–269.
  51. 51. DeSouza O, Araújo APA, Reis-Jr R (2009) Trophic controls delaying foraging by termites: reasons for the ground being brown? Bull Entomol Res 99: 603–609.
  52. 52. Kneitel JM, Chase JM (2004) Trade-offs in community ecology: linking spatial scales and species coexistence. Ecol Lett 7: 69–80.
  53. 53. Hubbell SP (2001) The Unified Neutral Theory of Biodiversity and Biogeography. Princeton and Oxford: Princeton University Press.
  54. 54. Coles HR (1980) Defensive strategies in the ecology of Neotropical termites. Ph.D. thesis, University of Southampton, Southampton, Hampshire.
  55. 55. Connell JH (1980) Diversity and the coevolution of competitors, or the ghost of competition past. Oikos 35: 131–138.
  56. 56. Noirot CH (1970) The nests of termites. In: Krishna K, Weesner F, editors, Biology of Termites, New York and London: Academic Press, volume 2: 73–125.
  57. 57. Jouquet P, Lepage M, Velde B (2002) Termite soil preferences and particle selections: strategies related to ecological requirements. Insectes Soc 49: 1–7.