Data on prevalence and incidence of hepatitis B virus (HBV) and hepatitis C virus (HCV) infection in Rwanda are scarce.
HBV status was assessed at baseline and Month 12, and anti-HCV antibodies at baseline, in a prospective cohort study of HIV-infected patients in Kigali, Rwanda: 104 men and 114 women initiating antiretroviral therapy (ART) at baseline, and 200 women not yet eligible for ART.
Baseline prevalence of active HBV infection (HBsAg positive), past or occult HBV infection (anti-HBc positive and HBsAg negative) and anti-HCV was 5.2%, 42.9%, and 5.7%, respectively. The active HBV incidence rate was 4.2/1,000 person years (PY). In a multivariable logistic regression model using baseline data, participants with WHO stage 3 or 4 HIV disease were 4.19 times (95% CI 1.21–14.47) more likely to have active HBV infection, and older patients were more likely to have evidence of past exposure to HBV (aRR 1.03 per year; 95%CI 1.01–1.06). Older age was also positively associated with having anti-HCV antibodies (aOR 1.09; 95%CI 1.04–1.14) while having a higher baseline HIV viral load was negatively associated with HCV (aOR 0.60; 95% CI 0.40–0.98). The median CD4 increase during the first 12 months of ART was lower for those with active HBV infection or anti-HCV at baseline. Almost all participants (88%) with active HBV infection who were on ART were receiving lamivudine monotherapy for HBV.
HBV and HCV are common in HIV-infected patients in Rwanda. Regular HBsAg screening is needed to ensure that HIV-HBV co-infected patients receive an HBV-active ART regimen, and the prevalence of occult HBV infection should be determined. Improved access to HBV vaccination is recommended. Active HCV prevalence and incidence should be investigated further to determine whether HCV RNA PCR testing should be introduced in Rwanda.
Citation: Rusine J, Ondoa P, Asiimwe-Kateera B, Boer KR, Uwimana JM, Mukabayire O, et al. (2013) High Seroprevalence of HBV and HCV Infection in HIV-Infected Adults in Kigali, Rwanda. PLoS ONE 8(5): e63303. doi:10.1371/journal.pone.0063303
Editor: Jason Blackard, University of Cincinnati College of Medicine, United States of America
Received: December 17, 2012; Accepted: April 1, 2013; Published: May 22, 2013
Copyright: © 2013 Rusine et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was part of the INTERACT Program funded by the Netherlands-African Partnership for Capacity Development and Clinical Interventions against Poverty-related Diseases (NACCAP) of the Netherlands Organisation for Scientific Research – The Netherlands Foundation for the Advancement of Tropical Research (NWO/WOTRO; URL: http://www.nwo.nl/nwohome.nsf/pages/NWOP_5VWBMM_Eng). Funding was also received from the European and Developing Countries Clinical Trials Partnership (EDCTP) as part of a project entitled: ‘Preparing for Phase III vaginal microbicide trials in Rwanda and Kenya: Preparedness studies, capacity building, and strengthening of medical referral systems’ (CT_ct_05_33070_001; URL: http://www.edctp.org/). The views expressed in this paper are those of the authors and do not necessarily represent the official position of NWO-WOTRO or EDCTP. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
In sub-Saharan Africa, 65–98% of the population will have lifetime exposure to hepatitis B virus (HBV) and 8–20% will become a chronic carrier . The predominant modes of transmission are perinatal and horizontal (in early childhood), but transmission via unprotected sexual intercourse or intravenous drug use in adults also occurs , . The proportion progressing from acute to chronic HBV is primarily determined by the age at infection: approximately 90 percent for perinatal infection, 20–50 percent for early childhood infection, and less than 5 percent for adult infection . Hepatitis C virus (HCV) is a parenterally transmitted virus . Sexual and vertical transmission of HCV is considered inefficient but co-infection of HIV and HCV increases the risk of perinatal transmission of either virus .
Between 2 and 20% of HIV-positive individuals in sub-Saharan Africa are also infected with HBV –. The consequences of co-infection are increased liver-related morbidity and mortality, increased viral replication of either virus and, in the context of antiretroviral therapy for HIV (ART), immune reconstitution inflammatory syndrome (IRIS) and hepatotoxicity , . In recent years, lamivudine, emtricitabine, and tenofovir have been approved for the management of HIV/HBV co-infection. This raises a number of possibilities and concerns related to the management of these infections. Studies have demonstrated that anti-HBV-active ART makes it possible to achieve suppression of HBV replication in a significant number of co-infected patients . On the other hand, the HBV status of most HIV patients in Africa is not known, which means that many are unknowingly receiving mono-therapy for HBV infection in the context of their ART for HIV. Mono-therapy with lamivudine has been shown to induce HBV resistance in 24% of HBV mono-infected patients after one year, increasing to 71% after 5 years of treatment . Moreover, resistance to lamivudine confers partial or complete cross-resistance to the HBV inhibitors emtricitabine, telbivudine and entecavir, thus limiting future treatment options for HBV infection. In contrast, an ART backbone of tenofovir/emtricitabine or tenofovir/lamivudine could be expected to suppress HBV replication effectively while inducing less HBV resistance .
HIV and HCV co-infections are likely to be less common in sub-Saharan Africa due to the differences in transmission routes but only limited data are available . HIV infection, alcohol consumption, and older age at the time of HCV infection have been shown to be associated with a higher rate of liver fibrosis progression . Treatment for HCV with pegylated interferon is rarely available in sub-Saharan African public health care settings.
Data on the prevalence and incidence of HBV and HCV infection in Rwandan HIV-infected adults are scarce. One study among HIV-infected pregnant women in 2007 found a seroprevalence of 2.4% for active HBV and 4.9% for anti-HCV antibodies . Since 2011, the Rwandan government recommends that all HIV patients co-infected with HBV receive an ART regimen containing tenofovir and lamivudine or emitricitabine  but HIV patients are not routinely tested for HBV. We observed HIV patients receiving care at a public HIV clinic in Kigali, Rwanda, between 2007 and 2010, and documented various behavioral, clinical, and laboratory endpoints (the SEARCH study). Within this context, we determined the prevalence and determinants of active and past HBV infection and anti-HCV antibodies and the incidence of active HBV infection, assessed the effect of HBV and HCV infection on HIV disease progression, and described the ART regimens that patients with active HBV co-infection are currently receiving.
The SEARCH study (Side Effects and Reproductive Health in a Cohort on HAART) was a prospective cohort study in Kigali, Rwanda, to evaluate ART adherence and outcomes, and the impact of ART on various aspects of reproductive and sexual health, including HBV and HCV. The SEARCH study was conducted at the HIV outpatient clinic of the Center for Treatment and Research on AIDS, Tuberculosis and Malaria (TRAC-Plus), which is now part of the Institute of HIV/AIDS, Disease Prevention and Control (IHDPC) within the Rwanda Biomedical Center. Ethical approval was obtained from the Rwandan National Ethics Committee. All study participants provided written informed consent prior to enrollment, were free to withdraw from the study at any time, and continued to receive care within publicly-funded HIV treatment programs at the end of their study participation.
Study Design and Population
The SEARCH study was designed to enroll 100 women and 100 men initiating ART and 200 women who did not yet qualify for ART according to the Rwanda Ministry of Health guidelines because they had a CD4 count higher than 350 cells/µl and were asymptomatic . All eligible patients attending the TRAC-Plus clinic between November 2007 and January 2010 were offered enrollment into the study. Patients were followed up for 6 to 24 months, until the study was closed in August 2010. Only data collected during the first 12 months of follow-up are presented in this paper. The study visit schedule followed the national ART program schedule as much as possible so that study participants could easily be transferred from SEARCH to the national ART program when the study ended. Women not eligible for ART were seen at baseline and 3-monthly intervals thereafter, and they were switched to the ART cohort when they became eligible for ART. For patients initiating ART, clinic visits were scheduled at baseline (ART initiation) and at week 2 and months 1, 2, 3, 6, 9, and 12 after ART initiation. In addition, pharmacy visits were scheduled monthly.
The study population consisted of ART-naïve HIV patients, 18 years or older, seeking care at the TRAC-Plus clinic. ART eligibility was determined by a TRAC-Plus committee according to the Rwanda Ministry of Health guidelines and clinic protocols. Study eligibility criteria also included residing within travel distance from the TRAC-Plus clinic, and being willing and able to adhere to the study protocol and give informed consent. The main exclusion criteria were pregnancy and diagnosis or clinical suspicion of tuberculosis.
All participants were interviewed regularly throughout the study to collect information on demographics, sexual and contraceptive behavior, nutritional status, medical and reproductive history, use of medications, and symptoms (with a focus on those potentially related to HIV infection, ART use or reproductive health problems). Clinical assessments were also carried out at most visits and included a general physical exam, a speculum exam in women, and targeted assessments for neuropathy and lipodystrophy. Blood samples were collected at all clinic visits. They were hand-carried from the clinic to the laboratory next door within 4 hours after collection. CD4+ T-cell counts on EDTA blood samples were always done on the day of sample collection. All other samples were centrifuged to separate plasma from blood cells, aliquoted and stored at −80°C on the day of sample collection until further processing and testing.
All laboratory tests relevant to this paper were carried out at the National Reference Laboratory in Kigali, Rwanda. At study enrollment, the presence of HIV infection in each study participant was confirmed using a 4th generation HIV ELISA. CD4+ T cell counts (FACSCalibur, Becton Dickinson, San Jose, CA, USA) were done every 3 months for women who did not yet qualify for ART and every 6 months for those initiating ART. Plasma HIV RNA viral loads (COBAS AmpliPrep/COBAS TaqMan HIV-1 Test versions 2.0, Roche Molecular Diagnostics, Pleasanton, CA, USA) were done at ART initiation and every 12 months thereafter. The lower limit of detection was 40 HIV RNA copies/ml.
An hCG urine pregnancy test was done every 6 months and testing for various sexually transmitted infections (STIs) every 6 or 12 months. Herpes simplex type 2 (HSV-2) serology was done every 12 months using HerpeSelect test kits (Focus Diagnostics, Cypress, CA, USA). The liver enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) (COBAS Integra Chemistry Analyzer, Roche Diagnostics, Mannheim, Germany) were determined at all visits post ART initiation.
HBV and HCV testing was conducted on all available baseline and Month 12 specimens after data collection had been completed and the study had been closed. About 4 mL plasma in EDTA Vacutainer tubes had been stored at −80°C. After thawing, specimens were tested for HBV surface antigen (HBsAg) using the Murex HBsAg version 3 kit, anti-HBV core antibody (anti-HBc) using the Murex anti-HBc total kit, and anti-HCV IgG antibody (anti-HCV) using the Murex anti-HCV version 4 kit (for all: Abbott Murex, Dartford, UK). However due to a kit shortage, 38 specimens were tested on the Abbott ARCHITECT i2000/SR (Abbott Diagnostics, Lake Forest, IL, USA) using test kits with similar specificity and sensitivity as the Murex kits. All assays were performed according to manufacturer instructions. HBV DNA PCR and HCV RNA PCR were not available in Rwanda at the time of the study. Participants with clinically relevant abnormal test results for HBV and HCV were referred to Kigali University Teaching Hospital, Department of Gastro-enterology for clinical follow-up.
Data were analyzed using STATA version 11.0 (StataCorp, College Station, TX, USA). Stem and leaf plots and Shapiro-Wilk tests were used to investigate the normality of data distribution. The Pearson Chi-square test, student’s t-test or ANOVA test were used to test for differences between groups in the case of normally distributed data, and non-parametric tests were used otherwise.
A positive test for HBsAg, regardless of anti-HBc test result, was interpreted as active HBV infection (acute or chronic). A positive test for anti-HBc in combination with a negative test for HBsAg is referred to as a past infection in this manuscript, but we could not differentiate past infections from occult infections. Any exposure to HBV was defined as a positive test for anti-HBc with or without a positive HBsAg test. A positive test for anti-HCV antibodies was interpreted as evidence of past or ongoing HCV infection. Incident HBV infection was defined as having a positive HBsAg test at Month 12 among participants with a negative HBsAg test at baseline. Virological failure to ART was defined as having more than 40 HIV RNA copies/mL 12 months after ART initiation.
Potential determinants of HBV infection were assessed in bi- and multivariable models using multinomial logistic regression with active HBV infection (HBsAg positive regardless of anti-HBc result), past HBV infection (anti-HBc positive and HBsAg negative), and never having been HBV exposed (negative for both) as the outcome variable. Potential determinants of anti-HCV antibodies were assessed in bi- and multivariable binary logistic regression models. In all multivariable models, potential determinants were selected based on evidence from published studies (age, gender), Akaike’s information criteria, and a p-value of less than 0.2 in bivariable models. No evidence for collinearity was found in least squares regression models containing the same variables as our logistic regression models (all variance inflation factors were below 2). Sensitivity analyses were conducted to determine the robustness of the models by excluding participants who were anti-HCV positive at baseline from the analyses with HBV as the outcome. This did not alter any of our conclusions and results are therefore not presented.
Four hundred and eighteen participants were enrolled into this study: 104 men and 114 women initiating ART, and 200 women who were not yet eligible for ART. A total of 16 participants were excluded from the baseline analyses due to erroneous enrollment or missing blood specimens or test results (Figure 1). During follow-up, 4 participants died, 13 transferred from the pre-ART to the ART group, 11 were lost to follow-up, 103 had not yet reached the Month 12 visit when the study was terminated, and 18 had missing HBV test results at Month 12. The analysis sample therefore consisted of 402 participants at baseline, and 253 of them (63%) had HBV test results at 12 months of follow up.
The mean age of study participants was 34.6 years, and those who had never been exposed to HBV were significantly younger than those who had been exposed to HBV or HCV (Table 1). More than half (56.9%) of the participants were married, and most had attended primary (50.2%) or secondary (35.8%) school. Eight percent of the participants reported to have two or more sexual partners, 47.0% used a condom during their last vaginal sex act, and 38.1% regularly consumed alcohol (defined as alcohol use on more than 3 days per week) during the previous 6 months. Injection drug use was not reported by anyone.
Most participants had a normal body mass index (BMI; 57.3%) and those with an abnormal BMI were more often overweight or obese (32.9%) than underweight (9.8%). The majority of participants (85.8%) had positive HSV-2 serology. Most participants were in WHO clinical stage 1, but the distribution of clinical stage varied significantly between the HBV and HCV outcome groups (see Table 1 and further). All participants who initiated ART at baseline and who had a Month 12 follow-up visit were given a first-line regimen that contained lamivudine but not tenofovir, which was in agreement with the national treatment guidelines at that time (Table 1). Participants initiating ART had a baseline median CD4 count of 222 cells/µl (interquartile range (IQR) 142–289) and a median HIV RNA viral load of 4.81 log10 copies/ml (IQR 4.22–5.33). Participants not initiating ART had a baseline median CD4 count of 513 cells/µl (IQR 413–651) and a median HIV RNA viral load of 3.69 log10 copies/ml (IQR 2.92–4.41).
Prevalence and Incidence of HBV and HCV
The baseline prevalence of active HBV was 5.2% (21/402; 95% CI 3.0–7.4), past HBV 42.9% (172/401; 95% CI 38.0–47.8) and never having been exposed to HBV 51.9% (208/401; 95% CI 47.0–56.7). Of the 21 cases of active HBV at baseline, 6 were HBsAg-negative at Month 12, 10 were still HBsAg-positive, and 5 were not retested. Between baseline and Month 12, one new case of active HBV was detected, giving an incidence rate of 4.2/1,000 person years of follow-up (PY) (95% CI 0/1,000–12.5/1,000 PY). The baseline prevalence of anti-HCV was 5.7% (23/402; 95% CI 3.44–8.0%). At baseline, 3 participants tested positive for both HBsAg and anti-HCV and 12 for both anti-HBc and anti-HCV.
Baseline Determinants of HBV and HCV Infection
In the multivariable model, participants with active HBV infection presented with a more advanced WHO clinical stage (stage 3 or 4 compared to stage 1; adjusted RR 4.19; 95% CI 1.21–14.47) than participants who had never been exposed to HBV (Table 2). Participants with past HBV infection were more likely to be older than those who had never been exposed to HBV with a 3% increased risk for each year increase in age (adjusted RR 1.03; 95% CI 1.01–1.06). Baseline CD4 counts and HIV viral loads were not significantly different between the three HBV outcome groups (Tables 1 and 2).
In the multivariable HCV model, a year increase in age led to a 10% increased odds of being anti-HCV positive (adjusted OR 1.10, 95% CI 1.05–1.17) (Table 2). A more advanced WHO clinical stage was positively associated with anti-HCV (stage 3 or 4 compared to stage 1; adjusted OR 4.05; 95% CI 1.12–14.58) but a higher baseline HIV viral load was negatively associated with anti-HCV (adjusted OR 0.60; 95% CI 0.40–0.98).
ART Regimens Taken by HIV-HBV Co-infected Patients
Among those with active HBV at baseline (n = 21), 16 were in the group that initiated ART immediately: 14 initiated an ART regimen containing lamivudine but not tenofovir and 2 initiated a regimen including tenofovir. Only the 14 patients not using tenofovir were retested at Month 12∶4 were HBsAg-negative and anti-HBc positive at Month 12 and 10 developed persistent infection. One person switched to tenofovir at the Month 12 visit but this switch was related to drug toxicity and not to the patient’s HBV status. Of the 5 HIV-HBV co-infected patients who did not initiate ART at baseline, only 2 were retested at Month 12. Both of them were HBsAg-negative and anti-HBc positive at Month 12.
Effects of HBV and HCV on Liver Enzymes and HIV Disease Progression
Liver enzymes were monitored only after participants had initiated ART. No statistical differences in median ALT and AST levels at baseline and Month 12 were found between the different HBV and HCV outcome groups (Table 3).
Among those who had initiated ART, participants with active HBV at baseline achieved a median CD4 increase of only 28 cells/µl/year while participants who had never been exposed to HBV achieved a median increase of 113 cells/µl/year (p = 0.04). There was no statistically significant difference in the median CD4 increase between participants who had been infected with HBV in the past (127 cells/µl/year) and those who had never been exposed (113 cells/µl/year). Among those who had initiated ART, 69% of participants with active HBV or past HBV exposure at baseline achieved full HIV viral load suppression (defined as HIV RNA levels below 40 copies/ml) after 12 months of ART compared to 83% of the participants who had never been exposed to HBV (p = 0.11). In a multivariable analysis controlled for baseline CD4 count and baseline HIV viral load, participants with active HBV infection or past exposure to HBV at baseline had 2.40 increased odds of virological failure compared to non-HBV exposed participants (OR 2.40; 95% CI 1.04–5.51).
Participants with anti-HCV at baseline had a slower CD4 count recovery rate of 58 cells/µl/year compared to participants without anti-HCV (126 CD4 cells/µl/year; p = 0.07). HIV virological failure did not differ significantly between participants with anti-HCV (60%) and those without (76%; p = 0.26).
The prevalence rates of active HBV infection and anti-HCV in this cohort of HIV patients seeking care in an urban HIV clinic in Rwanda were high at 5.2% and 5.7%, respectively. Almost half of all patients (42.9%) had ever been exposed to HBV. These rates are slightly higher than those reported in 2007 for HIV-positive pregnant women in Rwanda (2.4% for active HBV and 4.9% for anti-HCV) . High rates have also been found in other African settings –. In fact, several African studies have found even higher rates for HBsAg prevalence in HIV-positive patients than we have –.
The major limitation of our study is that we could not identify occult HBV infections in those who were HBsAg-negative but anti-HBc-positive by HBV DNA PCR testing, and that we could not identify active HCV infections among those positive for anti-HCV by HCV RNA PCR testing. Occult active HBV infections are thought to be common among HIV patients, with reported prevalence rates ranging from 10–14% – to 89% in one study in South Africa . Given the fact that 42.9% of HIV patients in Rwanda had isolated anti-HBc, further research is needed to determine the prevalence of occult HBV in this group. Similarly, further research is needed to determine the prevalence of active HCV infection among those with anti-HCV. About 25% of HCV infections clear spontaneously (resulting in a positive anti-HCV test but a negative HCV RNA PCR test) . Furthermore, while anti-HCV testing has been used extensively to estimate HCV prevalence and incidence, recent studies comparing antibody tests with HCV RNA PCR suggest that false-positive antibody test results are common in African settings . Unfortunately, routine confirmation of positive anti-HCV test results with HCV RNA PCR is expensive and not feasible in many African settings. Larger studies designed to determine the sensitivity and specificity of HCV assays, and to evaluate affordable diagnostic algorithms, in African settings are urgently needed.
Other limitations of our study included the fact that ALT and AST were only evaluated in patients after they had initiated ART and the fact that 37% of the patients had less than 12 months follow-up due to study closure. A strength of our study is that we worked within a public HIV care setting and are therefore reporting results that are relevant to local policymakers.
In our study, older age was associated with past exposure to HBV infection and with exposure to HCV infection. This most likely reflects ongoing exposure over time, as has been reported by others , . Gender was, however, not associated with active HBV, past HBV or anti-HCV in our study, which is in contrast with several other studies that have shown strong associations with male gender , , , . This could be explained by the fact that our study oversampled women by design and did not include any injecting drug users. We did not find any associations between HBV and sexual risk behaviors, which is also in contrast with other studies , , . In our cohort, sexual risk taking was modest, with 8.2% of the participants reporting multiple partners and 47.0% reporting to have used a condom during the last sex act. We did not ask the male participants whether they have had sex with other men.
WHO clinical stage 3 or 4 at baseline was associated with a higher likelihood of having an active HBV or anti-HCV at baseline, but while the median baseline CD4 count was lower among those with active HBV (but not among those with anti-HCV), this was not statistically significant. However, having active HBV or anti-HCV at baseline were both associated with a slower CD4 count recovery rate during follow-up. This has also been found in a study in Ghana , but not in studies in South Africa, Nigeria, and Denmark –. Surprisingly, having a detectable HIV RNA viral load at baseline was less likely in those who had HCV antibodies at baseline. This has not been confirmed by other studies , and should be interpreted with caution because we only conducted anti-HCV testing and did not confirm infections with HCV RNA PCR. HIV virological failure after 12 months of ART was more likely in those with active HBV at baseline but not in those with anti-HCV. Reports in the literature about virological failure rates in those with and without active HBV are conflicting –. Finally, we did not find any statistical differences in median ALT and AST levels between the different HBV and HCV outcome groups, but the comparison groups were small. Reports in the literature in this area are also conflicting –, , . Additional carefully designed studies investigating the impact of HBV and HCV co-infection on ART response and on hepatotoxicity are therefore needed.
During the first 12 months of the study, almost all participants on ART were using a regimen containing lamivudine but not tenofovir, which was in accordance with the Rwanda antiretroviral treatment guidelines for HIV infection prior to the revision in 2011. Of the participants with HIV and active HBV co-infection who were on ART, 88% were receiving lamivudine monotherapy for their HBV infection. Studies have shown that treatment with two anti-HBV-active drugs simultaneously reduces the emergence of drug resistance –.
In conclusion, active HBV infection and anti-HCV are both common in HIV-infected patients in Rwanda and are associated with a slower rate of CD4 recovery in the context of HIV-suppressive ART. Our study confirms the importance of screening HIV patients in Rwanda for HBsAg to ensure that HIV-HBV co-infected patients receive an HBV-active ART regimen. The prevalence of occult HBV infection should be determined and improved access to HBV vaccination considered. Active HCV prevalence and incidence should be investigated further to determine whether HCV RNA PCR testing should be introduced in Rwanda.
We thank the SEARCH study participants, the SEARCH team at the TRAC-Plus clinic, the INTERACT Rwanda office, and the National Reference Laboratory and National Blood Transfusion Center in Rwanda. We also thank Nienke Veldhuijzen, Coosje Tuijn, Robert Meester, Diana Numan, Ludwig de Naeyer, and Andrew Musemakweli.
Conceived and designed the experiments: JW PR PO. Performed the experiments: JR PO JMU. Analyzed the data: BK JR KB PO JW. Contributed reagents/materials/analysis tools: OM. Wrote the paper: JW JR BK PO. Clinical evaluation of the patients in Rwanda: JM. Guidance on interpretation of hepatitis testing results: HZ.
- 1. Alter MJ (2003) Epidemiology of hepatitis B in Europe and worldwide. J Hepatol 39 (Suppl 1)S64–69.
- 2. Edmunds WJ, Medley GF, Nokes DJ, O’Callaghan CJ, Whittle HC, et al. (1996) Epidemiological patterns of hepatitis B virus (HBV) in highly endemic areas. Epidemiol Infect 117: 313–325.
- 3. Vardas E, Mathai M, Blaauw D, McAnerney J, Coppin A, et al. (1999) Preimmunization epidemiology of hepatitis B virus infection in South African children. J Med Virol 58: 111–115.
- 4. Lavanchy D (2004) Hepatitis B virus epidemiology, disease burden, treatment, and current and emerging prevention and control measures. J Viral Hepat 11: 97–107.
- 5. Shepard CW, Finelli L, Alter MJ (2005) Global epidemiology of hepatitis C virus infection. Lancet Infect Dis 5: 558–567.
- 6. Arshad M, El-Kamary SS, Jhaveri R (2011) Hepatitis C virus infection during pregnancy and the newborn period – are they opportunities for treatment? J Viral Hepat 18: 229–236.
- 7. Burnett RJ, François G, Kew MC (2005) Hepatitis B virus and human immunodeficiency virus co-infection in sub-Saharan Africa: a call for further investigation. Liver Int 25: 201–213.
- 8. Barth RE, Huijgen Q, Taljaard J, Hoepelman AI (2010) Hepatitis B/C and HIV in sub-Saharan Africa: an association between highly prevalent infectious diseases. A systematic review and meta-analysis. Int J Infect Dis 14: e1024–e1031.
- 9. Hoffmann CJ, Thio CL (2007) Clinical implications of HIV and hepatitis B co-infection in Asia and Africa. Lancet Infect Dis 7: 402–409.
- 10. Thio CL, Seaberg EC, Skolasky R Jr, Phair J, Visscher B, et al. (2002) HIV-1, hepatitis B virus, and risk of liver-related mortality in the Multicenter Cohort Study (MACS). Lancet 360: 1921–1926.
- 11. Nunez M, Ramos B, Diaz-Pollan B, Camino N, Martín-Carbonero L, et al. (2006) Virological outcome of chronic hepatitis B virus infection in HIV-coinfected patients receiving anti-HBV active antiretroviral therapy. AIDS Res Human Retroviruses 22: 842–848.
- 12. Lok AS, Lai CL, Leung N, Yao GB, Cui ZY, et al. (2003) Long-term safety of lamivudine treatment in patients with chronic hepatitis B. Gastroenterol. 125: 1714–1722.
- 13. van Bommel F, Zollner B, Sarrazin C, Spengler U, Hüppe D, et al. (2006) Tenofovir for patients with lamivudine-resistant hepatitis B virus (HBV) infection and high HBV DNA level during adefovir therapy. Hepatol 44: 318–325.
- 14. Taylor LE, Swan T, Mayer KH (2012) HIV coinfection with hepatitis C virus: evolving epidemiology and treatment paradigms. Clin Infect Dis (Suppl 1): S33–S42.
- 15. Pirillo MF, Bassani L, Germinario EA, Mancini MG, Vyankandondera J, et al. (2007) Seroprevalence of hepatitis B and C viruses among HIV-infected pregnant women in Uganda and Rwanda. J Med Virol 79: 1797–1801.
- 16. Rwanda Ministry of Health (2011) National HIV Care and Treatment Guidelines, 2011.
- 17. Harania RS, Karuru J, Nelson M, Stebbing J (2008) HIV, hepatitis B and hepatitis C coinfection in Kenya. AIDS 19: 1221–1222.
- 18. Nakwagala FN, Kagimu MM (2002) Hepatitis B virus and HIV infections among patients in Mulago hospital. East Afr Med J 79: 68–72.
- 19. Di Bisceglie AM, Maskew M, Schulze D, Reyneke A, McNamara L, et al. (2010) HIV-HBV coinfection among South African patients receiving antiretroviral therapy. Antivir Ther 15: 499–503.
- 20. Firnhaber C, Viana R, Reyneke A, Schultze D, Malope B, et al. (2009) Occult hepatitis B virus infection in patients with isolated core antibody and HIV co-infection in an urban clinic in Johannesburg, South Africa. Int J Infect Dis 13: 488–492.
- 21. Laurent C, Bourgeois A, Mpoudi-Ngolé E, Kouanfack C, Ciaffi L, et al. (2010) High rates of active hepatitis B and C co-infections in HIV-1 infected Cameroonian adults initiating antiretroviral therapy. HIV Med 11: 85–89.
- 22. Otegbayo JA, Taiwo BO, Akingbola TS, Odaibo GN, Adedapo KS, et al. (2008) Prevalence of hepatitis B and C seropositivity in a Nigerian cohort of HIV-infected patients. Ann Hepatol 7: 152–156.
- 23. Diop-Ndiaye H, Toure-Kane C, Etard JF, Lô G, Diaw P, et al. (2008) Hepatitis B, C seroprevalence and delta viruses in HIV-1 Senegalese patients at HAART initiation (retrospective study). J Med Virol 80: 1332–1336.
- 24. Geretti AM, Patel M, Sarfo FS, Chadwick D, Verheyen J, et al. (2010) Detection of highly prevalent hepatitis B virus coinfection among HIV-positive persons in Ghana. J Clin Microbiol 48: 3223–3230.
- 25. Nebbia G, Garcia-Diaz A, Ayliffe U, Smith C, Dervisevic S, et al. (2007) Predictors and kinetics of occult hepatitis B virus infection in HIV-infected persons. J Med Virol 79: 1464–1471.
- 26. Shire NJ, Rouster SD, Rajici N, Sherman KE (2004) Occult hepatitis B in HIV-infected patients. J Acquir Immune Defic Syndr 36: 869–875.
- 27. Chadwick D, Stanley A, Sarfo S, Appiah L, Ankcorn M, et al. (2013) Response to antiretroviral therapy in occult hepatitis B and HIV co-infection in West Africa. AIDS 27: 139–41.
- 28. Rao H-Y, Sun D-G, Yang R-F, Liu F, Wang J, et al. (2012) Outcome of hepatitis C infection in Chinese paid plasma donors: A 12-19-year cohort study. J Gastroenterol Hepatol 27: 526–532.
- 29. Seremba E, Ocama P, Opio CK, Kagimu M, Thomas DL, et al. (2010) Poor performance of hepatitis C antibody tests in hospital patients in Uganda. J Med Virol 82: 1371–1378.
- 30. Chadwick D, Ankcorn M, Sarfo F, Phillips R, Fox Z, et al. (2012) Outcomes of starting first-line antiretroviral therapy in hepatitis B virus/HIV-coinfected patients in Ghana. J Antimicrob Chemother: doi:10.1093/jac/dks333.
- 31. Hoffman CJ, Charalambous S, Martin DJ, Innes C, Churchyard GJ, et al. (2009) Hepatitis B virus infection and response to antiretroviral therapy (ART) in a South African ART program. Clin Infect Dis 49: 1268–1273.
- 32. Idoka J, Meloni S, Muazu M, Nimzing L, Badung B, et al. (2009) Impact of hepatitis B virus infection on human immunodeficiency virus response to antiretroviral therapy in Nigeria. Clin Infect Dis 49: 1268–1273.
- 33. Omland LH, Weis N, Skinhøj P, Laursen A, Christensen PB, et al. (2008) Impact of hepatitis B virus co-infection on response to highly active antiretroviral treatment and outcome in HIV-infected individuals: a nationwide cohort study. HIV Med 9: 300–306.
- 34. Ladep NG, Agbaji O, Agaba P, Badung P, Imade G, et al. (2007) Does immunological status affect the prevalence of Hepatitis C virus infection among HIV/AIDS patients? Niger J Med 16: 231–234.