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The Impact of Study Size on Meta-analyses: Examination of Underpowered Studies in Cochrane Reviews

The Impact of Study Size on Meta-analyses: Examination of Underpowered Studies in Cochrane Reviews

  • Rebecca M. Turner, 
  • Sheila M. Bird, 
  • Julian P. T. Higgins



Most meta-analyses include data from one or more small studies that, individually, do not have power to detect an intervention effect. The relative influence of adequately powered and underpowered studies in published meta-analyses has not previously been explored. We examine the distribution of power available in studies within meta-analyses published in Cochrane reviews, and investigate the impact of underpowered studies on meta-analysis results.

Methods and Findings

For 14,886 meta-analyses of binary outcomes from 1,991 Cochrane reviews, we calculated power per study within each meta-analysis. We defined adequate power as ≥50% power to detect a 30% relative risk reduction. In a subset of 1,107 meta-analyses including 5 or more studies with at least two adequately powered and at least one underpowered, results were compared with and without underpowered studies. In 10,492 (70%) of 14,886 meta-analyses, all included studies were underpowered; only 2,588 (17%) included at least two adequately powered studies. 34% of the meta-analyses themselves were adequately powered. The median of summary relative risks was 0.75 across all meta-analyses (inter-quartile range 0.55 to 0.89). In the subset examined, odds ratios in underpowered studies were 15% lower (95% CI 11% to 18%, P<0.0001) than in adequately powered studies, in meta-analyses of controlled pharmacological trials; and 12% lower (95% CI 7% to 17%, P<0.0001) in meta-analyses of controlled non-pharmacological trials. The standard error of the intervention effect increased by a median of 11% (inter-quartile range −1% to 35%) when underpowered studies were omitted; and between-study heterogeneity tended to decrease.


When at least two adequately powered studies are available in meta-analyses reported by Cochrane reviews, underpowered studies often contribute little information, and could be left out if a rapid review of the evidence is required. However, underpowered studies made up the entirety of the evidence in most Cochrane reviews.


Systematic reviews of intervention studies aim to synthesise all available evidence meeting pre-specified eligibility criteria. Such criteria seldom address sample size. Meta-analyses may therefore include data from one or more small studies which, individually, do not have power to detect a modest intervention effect. Small studies tend to report greater intervention effects than larger studies [1]. So-called “small-study effects” may arise from reporting biases, whereby findings in smaller studies are more likely to be selected for publication on the basis of statistical significance [2]. Alternatively, small-study effects may arise from biases caused by methodological flaws arising more frequently in small studies [3], or may be due to true differences in the underlying effects between smaller and larger studies.

Some researchers argue for excluding small studies from meta-analyses. Specifically to reduce the effects of publication bias, Stanley suggested discarding 90% of the study estimates, so that conclusions are based on only the most precise 10% of studies [4]. Earlier, Kraemer proposed including only adequately powered studies in meta-analysis, both to remove publication bias and to discourage future researchers from carrying out small studies [5]. In teaching, Bird has long advocated that trials should not be started unless they could deliver at least 50% power in respect of a priori plausible, worthwhile effect sizes [6]. The prospect of inclusion in later meta-analyses may partly explain why investigators continue to feel justified in conducting underpowered studies [7][9]. Researchers who choose to undertake a study that is capable of detecting only an unrealistically large effect may lack understanding of both scientific methods and ethics [10].

Arguments for including small studies in meta-analyses uphold that evidence synthesis is best informed by all reasonably unbiased evidence and that no such evidence should be discarded lightly. Cut-offs based on study size, although scientifically cost-efficient, introduce an extra element of subjectivity and might not ameliorate bias if the remaining large studies are insufficiently critiqued [11]. Moreover, observing heterogeneity in effects across multiple independent trials is important, even if some of these are smaller, since this is likely to reflect heterogeneity that would occur in clinical practice [12]; [13]. Difficulties caused by reporting biases and related small-study effects can be addressed through statistical methods of adjustment [14]; [15].

In this paper, we explore the levels of power available in studies included in published meta-analyses, and examine the relative influence of adequately powered and underpowered studies on these meta-analyses.



To examine power per study within meta-analyses and to explore whether this varies across different settings, we use evidence from the Cochrane Database of Systematic Reviews (CDSR: Issue 1, 2008), which was provided by the Nordic Cochrane Centre. Each meta-analysis was categorized by type of outcome, types of intervention compared, and medical specialty to which the research question related, as described elsewhere [16]. In this paper, we include all meta-analyses of binary outcomes that reported data from two or more studies (14,886 meta-analyses).

Calculation of Power per Study

In meta-analysis j, power was calculated with respect to a fixed baseline event rate, . The median of the observed proportions experiencing events was calculated for each intervention arm separately and the higher median was used as . For each study i within meta-analysis j (with mean number of patients per treatment arm), we calculated how much power the study sample size provided to detect a relative risk reduction of 10%, 20%, 30% or 50% (or, equivalently, a relative risk of  = 0.9, 0.8, 0.7 or 0.5). For convenience, we refer to a relative risk reduction of 30%, for example, as RRR30. In study i within meta-analysis j, the power to detect a difference between event rates and at a significance level of is given by:where is the cumulative standard normal distribution function and . For our primary analyses we define adequate power as ≥50% power to detect RRR30. In subsequent analyses, we fitted a random-effects model to obtain a summary relative risk estimate, , for meta-analysis j, and calculated the power of study i to detect the treatment effect observed in the meta-analysis to which it contributed, i.e. to detect a difference between and .

Calculation of power per Meta-analysis

The focus of this paper is on the power of primary studies within meta-analyses, but it is interesting also to examine the power of the meta-analyses themselves. In each meta-analysis j, we fitted a random-effects model, using a method-of-moments estimate for the between study variance [17], and calculated the variance Vj of the combined intervention effect (on the log relative risk scale). The power of meta-analysis j to detect a 30% relative risk reduction or equivalently a log relative risk of , using a significance level of is given by:where is the cumulative standard normal distribution function and [18].

Impact of Underpowered Studies

We defined subset A as CDSR meta-analyses that include five or more studies, with at least two adequately powered () with respect to RRR30 and at least one underpowered (), to investigate the impact of including or excluding underpowered studies. On the log odds ratio scale, per meta-analysis, we fitted fixed-effect and random-effects models including (1) all studies; (2) adequately powered studies only () or (3) underpowered studies only ().

For meta-analyses relating to beneficial rather than adverse outcomes, the data were rearranged, so that an odds ratio below 1 favours the experimental intervention over the comparator across all meta-analyses in subset A. A method-of-moments estimate was used for the between-study variance in the random-effects model [17].

As a descriptive analysis of the impact of excluding underpowered studies in subset A meta-analyses, we calculated ratios comparing meta-analysis results obtained from all studies with results from adequately powered studies only.

To compare effect sizes formally within subset A, we first estimated the average difference between log odds ratios in underpowered studies () compared with adequately powered studies () by fitting a random-effects meta-regression model. Then, in a random effects meta-analysis, we combined the estimated differences across subset A meta-analyses, with or without adjustment separately for (i) medical specialties, (ii) outcome type, (iii) intervention type. We also explored the role of underpowered studies in individual meta-analyses within a particular research setting in more detail, as described in Appendix S1.


Power of Studies Included in Cochrane Reviews

Table 1 summarizes power of primary studies within meta-analyses in the CDSR database. In 10,492 (70%) of the 14,886 CDSR meta-analyses, all studies were underpowered () to detect a 30% relative risk reduction (RRR30). In many settings, a 20% relative risk reduction would be more realistic, and 85% of the meta-analyses included no studies powered to detect RRR20. Only 2,588 (17%) meta-analyses included at least two studies powered at 50% or more to detect RRR30, and only 1,291 (9%) included at least two studies powered at 80% or more. Median power within CDSR meta-analyses was low for RRR30 at 13% power, with an inter-quartile range (iqr) of 7% to 31% power. Some studies were generously powered, with 2,571/77,237 (3.3%) having at least 98% power for RRR30 (Figure 1).

Figure 1. Distribution of power available to detect a relative risk reduction of 30%, across 77,237 studies.

Table 1. Percentages of 14,886 meta-analyses including no studies adequately powered to detect a target effect or including at least two adequately powered studies, where adequate power is defined as 80% or 50% in turn; and summary of median power within each meta-analysis.

Power of studies to detect the summary relative risk in their meta-analysis was also low: 11,422 (77%) meta-analyses included no studies with ≥50% power and only 2,236 (15%) meta-analyses included at least two studies with ≥50% power. The median of summary relative risks was 0.75 across all meta-analyses (iqr 0.55 to 0.89).

Table 2 summarizes power for RRR30 by medical specialty, outcome and intervention-comparison type. In cancer, 35% of 689 meta-analyses included at least two adequately powered studies, and only 365 meta-analyses (53%) consisted entirely of underpowered studies. However, median power within cancer meta-analyses remained low at 24% power (iqr 10% to 57% power).

Table 2. Numbers of adequately powered studies (≥50% power) and median power within each meta-analysis (MA) with respect to a 30% relative risk reduction (RRR30), overall and by medical specialty, outcome type and intervention-comparison type.

By outcome, we expected power to be lower for events that are typically rare. Power was indeed somewhat lower for meta-analyses reporting all-cause mortality and cause-specific mortality/major morbidity event/composite (mortality or morbidity), and somewhat higher for meta-analyses relating to resource use, signs/symptoms reflecting continuation/end of disease or a mixture of subjective outcomes (see Table 2).

Power of Meta-analyses Included in Cochrane Reviews

Table 3 summarizes the power of the meta-analyses themselves to detect a 30% relative risk reduction, overall and by medical specialty, outcome and intervention-comparison type. Overall, the proportion of meta-analyses with 80% power or more to detect RRR30 was 22%, with a further 12% powered at 50–80% to detect RRR30, but 66% were underpowered. At 34%, the proportion of adequately powered meta-analyses was substantially larger than the proportion of meta-analyses including at least two adequately powered studies, but remains low.

Table 3. Meta-analytic power with respect to a 30% relative risk reduction (RRR30), based on the random-effects model, overall and by medical specialty, outcome type and intervention-comparison type.

The median of meta-analytic power was 27% (iqr 11% to 72% power). There was some variation across medical areas; in cancer, 51% of meta-analyses were powered at 50% or more. Differences in meta-analytic power across medical areas, outcome and intervention-comparison types were largely in the same direction as differences in meta-analysis summaries of study power (Table 2).

Impact of Excluding Underpowered Studies from Meta-analyses

Of the 14,886 CDSR meta-analyses with binary outcomes, 1,107 (7.4%) were eligible for inclusion in subset A. The impact of excluding the underpowered trials on the results of these meta-analyses is summarised in Table 4. We calculated ratios comparing log odds ratio estimates from a meta-analysis of adequately powered studies only to those from a full meta-analysis. These are shown for fixed-effect and random-effects models separately.

Table 4. Ratios comparing results obtained from adequately powered studies only with results obtained from all studies, in subset A of 1,107 meta-analyses: results shown are percentiles of the distribution of such ratios across meta-analyses.

Across the 1,107 meta-analyses, there was a broad spread of ratios representing changes to the summary log odds ratio. The median ratio was 0.96 for the fixed-effect model and 0.94 for the random-effects model. The results correspond to a slight shift towards the null value when underpowered studies were removed, more so under the random-effects model in which small studies have greater influence.

Under the random-effects model, it is possible for precision to be gained (i.e. smaller standard error) when studies are removed, if the heterogeneity estimate is sufficiently reduced. The non-zero between-study heterogeneity in 851 meta-analyses decreased by a median of 21% when underpowered studies were removed (iqr −96% to +18%).

Table 5 presents average differences in log odds ratios between inadequately powered () and adequately powered studies, obtained from fitting meta-epidemiological models to the subset of 1,107 meta-analyses. Overall, the difference was −0.10 (95% CI −0.13 to −0.08, P<0.0001), which corresponds to odds ratios in underpowered studies being 10% lower on average (95% CI 8% to 13%), where lower odds ratios represent more extreme effects in favour of the active treatment. There was evidence that differences in log odds ratios varied across medical areas (P = 0.001), and across intervention-comparison types (P = 0.0002), but not by outcome types (P = 0.83). By medical area, the greatest differences between inadequately and adequately powered studies were observed for infectious diseases, mental health and behavioural conditions, gynaecology, pregnancy and birth, and in the mixed subset of “other medical specialties” (defined in footnote to Table 2). In comparisons of two active interventions, the results are less meaningful since the direction of the intervention effect is likely to vary across meta-analyses in the data set. Odds ratios in underpowered studies were 15% lower (95% CI 11% to 18%, P<0.0001) in meta-analyses comparing pharmacological interventions against control or placebo, and 12% lower (95% CI 7% to 17%, P<0.0001) in meta-analyses comparing non-pharmacological interventions against control or placebo.

Table 5. Average differences in observed log odds ratios between underpowered () compared to adequately powered studies, in subset A of 1,107 meta-analyses, overall and within medical specialties, outcome types and intervention-comparison types.

In Appendix S1, the role of underpowered studies in individual meta-analyses is explored in more detail.


Underpowered studies made up the entirety of the evidence in most meta-analyses reported by Cochrane reviews: in 70% of CDSR meta-analyses, all studies had less than 50% power to detect a 30% relative risk reduction (RRR30), and only 17% of meta-analyses included at least two studies with at least 50% power for RRR30. There was some variation across medical areas and outcome types, but individual studies’ power was low across all types of meta-analyses.

In a meta-epidemiological analysis of 1,107 meta-analyses, we found that odds ratios in underpowered studies were on average 10% lower (95% CI 8% to 12%, P<0.0001) than those in adequately powered studies. This should be regarded as a lower limit on the difference, since the database contains treatment comparisons that have underlying relative risks either side of 1. Indeed, the difference was larger among comparisons involving a control or placebo group (15% for controlled pharmaceutical trials), in which we might expect the direction of effect to be more consistent across meta-analyses. In meta-analyses in which at least two adequately powered studies are available, underpowered studies often had relatively little impact on the summary estimate of the odds ratio. The summary estimate shifted slightly toward the null when underpowered studies were removed, under both fixed-effect and random-effects models. The extent to which precision was lost when underpowered studies were excluded varied across meta-analyses. Some meta-analyses included a few very large studies, which dominated their results, while in other meta-analyses all studies were similarly sized and exclusion of underpowered studies led to greater losses in precision.

On average, the between-study heterogeneity estimate decreased when underpowered studies were excluded from meta-analyses, which may be expected since underpowered studies tend to observe more extreme effect estimates. Within the subset of 1,107 meta-analyses examined, the heterogeneity estimate sometimes decreased substantially when underpowered studies were removed. However, we also found examples where the heterogeneity increased when underpowered studies were removed, in settings where, for example, the largest studies in the meta-analysis had produced extremely different results.

The meta-analyses themselves were better powered than the primary studies within meta-analyses, as we would expect: overall, 34% of CDSR meta-analyses had at least 50% power to detect RRR30. Elsewhere, in the setting of cumulative meta-analysis in particular, the information size required for a meta-analysis to detect a particular effect size has been used to examine whether meta-analyses contain enough information to be conclusive [19][21]. Our finding that 22% of 14,886 meta-analyses were powered at 80% or more for RRR30 is comparable with, but much more precise than, the 39% of 174 meta-analyses from the Cochrane Neonatal Group, which were found to meet the information size criterion for RRR30 with 80% power by Brok et al. [20], who had, however, excluded both reviews with fewer than three trials and those in which all trials had a high risk of bias, in which meta-analytic size is likely to have been smaller.

Our work is limited to meta-analyses from Cochrane reviews, which may not be representative of meta-analyses in general. In particular, the differences observed between medical areas may reflect differing advice or editorial policies between Cochrane Review Groups which oversee different medical areas rather than disease-specific differences.

Although underpowered when included in meta-analyses, some original studies may have been adequately powered for their own primary outcomes, since the results extracted for meta-analysis might have related to secondary outcomes. For example, a study designed to detect a difference in measures of depression would be unlikely to be adequately powered for all-cause mortality. We do not therefore intend to criticise authors of primary studies for the very low levels of power in these meta-analyses. Publication dates of primary studies were not always available in the CDSR database, and so we were unable to look at the association between study age and power. It is possible that studies carried out in more recent years were more generously powered. However, the reasons for lack of power in completed studies include over-enthusiasm of researchers for the effectiveness of a new intervention, problems with recruitment to the study, and inaccurate sample size calculations [22]; these issues are common in experimental research and unlikely to disappear.

It is well known that small studies included in a meta-analysis tend to show more extreme treatment effects than larger studies. The differences observed between underpowered and adequately powered studies in the CDSR data set are consistent with previous findings, but offer much greater precision. For example, in a combined analysis of 13 meta-analyses evaluating effects on pain in patients with osteoarthritis, Nüesch et al. [23] found an average difference of −0.21 (95% CI −0.34 to −0.08) in standardized mean differences, when comparing trials with fewer than 100 patients per arm with larger trials. Several methods have been proposed for addressing small study effects in meta-analysis; recently, these were reviewed by Sterne et al. [24], who published new guidelines.

The practical implications of our findings for systematic reviews and meta-analyses vary according to review purpose and the research time available. Systematic reviews commissioned to inform public health policy decisions, by the National Institute for Health and Clinical Excellence (NICE) for example, are often carried out to tight deadlines [25]. Where a rapid review of the evidence is required and if several large, high-quality studies have been found in initial searches, it may be justifiable to truncate the searching and perform the synthesis, since inclusion of more obscure, smaller studies is unlikely to change the conclusions of the review. On the other hand, many Cochrane reviews are carried out in areas of scientific uncertainty, where discrepancies exist between findings from previous, mainly small studies. Here, the objective of meta-analysis is to resolve uncertainty by combining all available evidence and investigating reasons for between-study heterogeneity, and it would be inappropriate to leave out smaller studies. When carrying out a rapid meta-analysis to inform a grant application, the appropriate choice is less clear; although smaller studies might add little information relative to the time required for data extraction, it may be unethical to randomise yet more patients if a meta-analysis including small, existing studies would provide conclusive evidence.

In conclusion, we found that underpowered studies play a very substantial role in meta-analyses reported by Cochrane reviews, since the majority of meta-analyses include no adequately powered studies. In meta-analyses including two or more adequately powered studies, the remaining underpowered studies often contributed little information to the combined results, and could be left out if a rapid review of the evidence is required.

Supporting Information

Appendix S1.

Detailed exploration of the role of underpowered studies.



We are grateful to the Nordic Cochrane Centre (particularly Rasmus Moustgaard and Monica Kjeldstrøm) and the Cochrane Collaboration Steering Group for providing us with access to the Cochrane Database of Systematic Reviews. No ethical approval was required for this work.

Author Contributions

Conceived and designed the experiments: SMB JPTH. Performed the experiments: RMT SMB JPTH. Analyzed the data: RMT. Wrote the paper: RMT SMB JPTH.


  1. 1. Sterne JAC, Gavaghan D, Egger M (2000) Publication and related bias in meta-analysis: power of statistical tests and prevalence in the literature. Journal of Clinical Epidemiology 53: 1119–29.
  2. 2. Nygard O, Vollset SE, Refsum H, Stensvold I, Tverdal A, et al. (1995) Total plasma homocysteine and cardiovascular risk profile. JAMA 274: 1526–33.
  3. 3. Kjaergard LL, Villumsen J, Gluud C (2001) Reported methodologic quality and discrepancies between large and small randomized trials in meta-analyses. Annals of Internal Medicine 135: 982–9.
  4. 4. Stanley TD, Jarrell SB, Doucouliagos H (2010) Could it be better to discard 90% of the data? A statistical paradox. The American Statistician 64: 70–7.
  5. 5. Kraemer HC, Gardner C, Brooks III JO, Yesavage JA (1998) Advantages of excluding underpowered studies in meta-analysis: inclusionist versus exclusionist viewpoints. Psychological Methods 3: 23–31.
  6. 6. Merrall ELC, Kariminia A, Binswanger IA, Hobbs MS, Farrell M, et al. (2010) Meta-analysis of drug-related deaths soon after release from prison. Addiction 105: 1545–54.
  7. 7. Halpern SD, Karlawish JHT, Berlin JA (2002) The continuing unethical conduct of underpowered clinical trials. JAMA 288: 358–62.
  8. 8. Edwards SJL, Lilford RJ, Braunholtz D, Jackson J (1997) Why “underpowered” trials are not necessarily unethical. The Lancet 350: 804–7.
  9. 9. Guyatt GH, Mills EJ, Elbourne D (2008) In the era of systematic reviews, does the size of an individual trial still matter? PLoS Medicine 5(1): e4.
  10. 10. Altman DG (1994) The scandal of poor medical research. BMJ 308: 283.
  11. 11. Turner RM, Spiegelhalter DJ, Smith GCS, Thompson SG (2009) Bias modelling in evidence synthesis. Journal of the Royal Statistical Society, Series A 172: 21–47.
  12. 12. Shrier I, Platt RW, Steele RJ (2007) Mega-trials vs. meta-analysis: precision vs. heterogeneity? Contemporary Clinical Trials 28: 324–8.
  13. 13. Borm GF, Lemmers O, Fransen J, Donders R (2009) The evidence provided by a single trial is less reliable than its statistical analysis suggests. Journal of Clinical Epidemiology 62: 711–5.
  14. 14. Moreno SG, Sutton AJ, Ades AE, Stanley TD, Abrams KR, et al.. (2009) Assessment of regression-based methods to adjust for publication bias through a comprehensive simulation study. BMC Medical Research Methodology 9(2).
  15. 15. Rucker G, Carpenter JR, Schwarzer G (2011) Detecting and adjusting for small-study effects in meta-analysis. Biometrical Journal 53: 351–68.
  16. 16. Davey J, Turner RM, Clarke MJ, Higgins JPT (2011) Characteristics of meta-analyses and their component studies in the Cochrane Database of Systematic Reviews: a cross-sectional, descriptive analysis. BMC Medical Research Methodology 11: 160.
  17. 17. DerSimonian R, Laird N (1986) Meta-analysis in clinical trials. Controlled Clinical Trials 7: 177–88.
  18. 18. Hedges LV, Pigott TD (2001) The power of statistical tests in meta-analysis. Psychological Methods 6: 203–17.
  19. 19. Wetterslev JTK, Brok J, Gluud C (2008) Trial sequential analysis may establish when firm evidence is reached in cumulative meta-analysis. Journal of Clinical Epidemiology 61: 64–75.
  20. 20. Brok J, Thorlund K, Gluud C, Wetterslev J (2008) Trial sequential analysis reveals insufficient information size and potentially false positive results in many meta-analyses. Journal of Clinical Epidemiology 61: 763–9.
  21. 21. Thorlund K, Imberger G, Walsh M, Chu R, Gluud C, et al. (2011) The number of patients and events required to limit the risk of overestimation of intervention effects in meta-analysis - a simulation study. PLoS ONE 6(10): e25491.
  22. 22. Charles P, Giraudeau B, Dechartres A, Baron G, Ravaud P (2009) Reporting of sample size calculation in randomised controlled trials: review. BMJ 338: b1732.
  23. 23. Nuesch E, Trelle S, Reichenbach S, Rutjes AWS, Tschannen B, et al. (2010) Small study effects in meta-analyses of osteoarthritis trials: meta-epidemiological study. BMJ 341: c3515.
  24. 24. Sterne JAC, Sutton AJ, Ioannidis JPA, Terrin N, Jones DR, et al. (2011) Recommendations for examining and interpreting funnel plot asymmetry in meta-analyses of randomised controlled trials. BMJ 343: d4002.
  25. 25. Watt A, Cameron A, Sturm L, Lathlean T, Babidge W, et al. (2008) Rapid reviews versus full systematic reviews: an inventory of current methods and practice in health technology assessment. International Journal of Technology Assessment in Health Care 24: 133–9.