The binding of cholera toxin to the ganglioside GM1 as the initial step in the process leading to diarrhea is nowadays textbook knowledge. In contrast, the knowledge about the mechanisms for attachment of Vibrio cholerae bacterial cells to the intestinal epithelium is limited. In order to clarify this issue, a large number of glycosphingolipid mixtures were screened for binding of El Tor V. cholerae. Several specific interactions with minor complex non-acid glycosphingolipids were thereby detected. After isolation of binding-active glycosphingolipids, characterization by mass spectrometry and proton NMR, and comparative binding studies, three distinct glycosphingolipid binding patterns were defined. Firstly, V. cholerae bound to complex lacto/neolacto glycosphingolipids with the GlcNAcβ3Galβ4GlcNAc sequence as the minimal binding epitope. Secondly, glycosphingolipids with a terminal Galα3Galα3Gal moiety were recognized, and the third specificity was the binding to lactosylceramide and related compounds. V. cholerae binding to lacto/neolacto glycosphingolipids, and to the other classes of binding-active compounds, remained after deletion of the chitin binding protein GbpA. Thus, the binding of V. cholerae to chitin and to lacto/neolacto containing glycosphingolipids represents two separate binding specificities.
Citation: Benktander J, Ångström J, Karlsson H, Teymournejad O, Lindén S, Lebens M, et al. (2013) The Repertoire of Glycosphingolipids Recognized by Vibrio cholerae. PLoS ONE 8(1): e53999. https://doi.org/10.1371/journal.pone.0053999
Editor: Mikael Skurnik, University of Helsinki, Finland
Received: October 3, 2012; Accepted: December 7, 2012; Published: January 21, 2013
Copyright: © 2013 Benktander et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This study was supported by the Swedish Research Council, the Swedish Cancer Foundation, and governmental grants to the Sahlgrenska University Hospital. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Diarrheal disease caused by Vibrio cholerae remains a major health problem in many parts of the world, leading to 100 000 deaths annually . Infecting V. cholerae adhere to the small intestinal epithelium, and cause diarrhea primarily by the production of cholera toxin (CT). CT consists of one A-subunit with enzymatic activity, and five B-subunits mediating binding of the toxin to the small intestinal epithelium. In the early 1970s the GM1 ganglioside was identified as the receptor for CT . Since then a large collection of data about the molecular details of the GM1 binding by CT, and the mechanisms for induction of diarrhea, has accumulated .
There are more than 200 O-antigen serogroups of V. cholerae known to date, but epidemic cholera is caused only by strains belonging to the O1 and O139 serogroups. The V. cholerae O1 strains are divided into two biotypes, designated classical and El Tor, that have evolved from separate lineages . The first six pandemics were caused by the classical biotype, but after 1961 it has been displaced by the El Tor biotype. Recently, an evolution among El Tor strains with emergence of hybrid biotype strains with altered cholera toxin has been observed .
In contrast to the advanced information available about the interaction between CT and the GM1 ganglioside, the knowledge about the mechanisms for attachment of V. cholerae bacterial cells to the intestinal epithelium is sparse. Several hemagglutinins with roles in adherence have been isolated, but their precise binding specificities have not been defined . Recently, a chitin-binding protein (GbpA), which mediates attachment of V. cholerae to chitin surfaces, human intestinal cells and mouse intestine, was characterized , . These interactions are inhibited by GlcNAc, but since chitin per se is not present on the intestinal epithelium, the carbohydrate sequences required for GbpA-mediated intestinal attachment have not been elucidated.
In the present study we searched for V. cholerae carbohydrate recognition by binding of V. cholerae to a large number of glycosphingolipid mixtures. Several specific interactions with minor non-acid glycosphingolipids were thereby detected, and after isolation of binding-active glycosphingolipids, and characterization by mass spectrometry and 1H NMR, three distinct modes of glycosphingolipid binding were defined.
Materials and Methods
Vibrio cholerae Strains, Culture Conditions and Labeling
The El Tor V. cholerae strain JBK 70 (El Tor Inaba; CTA−/CTB−) , and the classical strain CVD103 (Classical Inaba; CTA−/CTB+) , were first cultured aerobically on Luria agar at 37°C for 12 h. Then colonies were inoculated in AKI medium , and incubated at 30°C with shaking under aerobic conditions for 12 h. For metabolic labeling, the medium (10 ml) was supplemented with 10 µl 35S-methionine (400 µCi; Amersham Pharmacia Biotech). The bacteria were harvested by centrifugation, washed three times with PBS (phosphate-buffered saline, pH 7.3), and resuspended in PBS containing 2% (w/v) bovine serum albumin, 0.1% (w/v) NaN3 and 0.1% (w/v) Tween 20 (BSA/PBS/TWEEN) to a bacterial density of 1×108 CFU/ml. The specific activity of the suspensions was approximately 1 cpm per 100 bacteria.
Construction of gbpA Deletion Mutant Strains
The gbpA genes in two different V. cholerae strains were disrupted by insertional mutagenesis using a suicide plasmid. One was the El Tor strain JBK70, and the other was the classical strain JS1569, a rifampicin resistant mutant of CVD103 .
A 520 base-pair fragment from the central region of the gbpA gene was amplified from strain JS1569 using primers gbpAf2 (5′-GGGGAGATCTAAACTCGCGTGTTTGATAACGAG-3′) and gbpAr2 (5′-GGGGAGATCTTGCAGATTGCCATCGCGATC-3′). The regions of the primers in italics are non-homologous tails that contain a recognition site for the restriction endonuclease XbaI. The underlined nucleotide in primer was changed from C to A in order to introduce a stop codon in the gbpA gene in addition to the insertion of suicide plasmid. The amplified DNA was digested with XbaI and ligated into the synthetic R6K-based suicide vector pMT-suicide1 . Ligated DNA was transformed into the E. coli strain S17-1 , and positive clones were isolated on the basis of restriction analysis of plasmids isolated from chloramphenicol resistant transformants. The resulting plasmid was then transferred into the recipient strains of V. cholerae by transconjugation accomplished by patch mating and selection of transconjugants by resistance to chloramphenicol and either rifampicin (JS1569) or polymyxin B (JBK70). The presence of the insert in the chromosomes of the resulting transconjugants was confirmed by PCR using primers gbpAf1 (5′-GCCAACCACGTCACAAAGGATTCC-3′) and gbpAr1 (5′-GAGTGGAGAGGTAGCCACTGGAG-3′) which gave a fragment 2.5 kb larger than the 1.2 kb wild-type due to insertion of the pMT-suicide1 plasmid and a repeat of a 520 bp fragment of the gbpA gene. The resulting V. cholerae strains were given the strain numbers 1382 (El Tor) and 1375 (classical). Culture and labeling of these gbpA deletion mutant strains was done using the same conditions as above, with addition of chloramphenicol 12.5 µg/ml to the agar plates and the AKI medium.
Pretreatment of Bacterial Cells
Suspensions of radiolabeled V. cholerae El Tor were prepared as described above, and suspended in PBS. Prior to incubation on thin-layer chromatograms the bacteria were subjected to one of the following treatments: (I) incubation at 37°C for 60 min; (II) incubation at 65°C for 60 min; (III) treatment with trypsin (Sigma) 1 mg/ml at 37°C for 60 min.
Chromatogram Binding Assays
Thin-layer chromatography was done on aluminum-backed silica gel 60 high performance thin-layer chromatography plates (Merck, Darmstadt, Germany). Glycosphingolipid mixtures (20–40 µg), or pure glycosphingolipids (1–4 µg), were applied to the plates, and eluted with chloroform/methanol/water (60∶35:8, by volume). Chemical detection was done with anisaldehyde .
Binding of radiolabeled bacteria to glycosphingolipids on thin-layer chromatograms was done as described . Dried chromatograms were dipped in diethylether/n-hexane (1∶5 v/v) containing 0.5% (w/v) polyisobutylmethacrylate for 1 min. The chromatograms were blocked with BSA/PBS/TWEEN for 2 h at room temperature. Then the plates were incubated for 2 h at room temperature with 35S-labeled bacteria (1–5×106 cpm/ml) diluted in BSA/PBS/TWEEN. After washing six times with PBS, and drying, the plates were autoradiographed for 12–36 h using XAR-5 x-ray films (Eastman Kodak, Rochester, NY). Chromatogram binding assays with 125I-labeled lectin from Erythrina cristagalli were done as described .
Isolation of El Tor Binding Glycosphingolipids
Total acid and non-acid glycosphingolipid fractions were isolated by standard methods . The non-acid glycosphingolipid fractions were separated by repeated silicic acid chromatography, and final separation was achieved by HPLC or by chromatography on Iatrobeads (Iatrobeads 6RS-8060; Iatron Laboratories, Tokyo) columns, eluted with chloroform/methanol/water 65∶25:4 (by volume), followed by chloroform/methanol/water 60∶35:8 (by volume), and finally chloroform/methanol/water 40∶40:12 (by volume). The fractions obtained were analyzed by thin-layer chromatography and anisaldehyde staining, and the V. cholerae El Tor binding activity was assessed using the chromatogram binding assay. The fractions were pooled according to the mobility on thin-layer chromatograms and their El Tor binding activity.
Endoglycoceramidase Digestion and Liquid Chromatography/Electrospray Ionization Mass Spectrometry
Endoglycoceramidase II from Rhodococcus spp.  (Takara Bio Europe S.A., Gennevilliers, France) was used for hydrolysis of glycosphingolipids, and the oligosaccharides obtained were analyzed by capillary-liquid chromatography mass spectrometry and tandem mass spectrometry . In brief, the oligosaccharides were separated on a column (200×0.180 mm) packed in-house with 5 µm porous graphite particles (Hypercarb, Thermo Scientific), and eluted with an acetonitrile gradient (A: 10 mM ammonium bicarbonate; B: 100% acetonitrile). The saccharides were analyzed in the negative ion mode on an LTQ linear quadrupole ion trap mass spectrometer (Thermo Electron, San José, CA). The IonMax standard ESI source on the LTQ mass spectrometer was equipped with a stainless steel needle kept at –3.5 kV. Compressed air was used as nebulizer gas. The heated capillary was kept at 270°C, and the capillary voltage was –50 kV. Full-scan (m/z 380–2 000, 2 microscans, maximum 100 ms, target value of 30 000) was performed, followed by data dependent MS2 scans of the three most abundant ions in each scan (2 microscans, maximum 100 ms, target value of 10 000). The threshold for MS2 was set to 500 counts. Normalized collision energy was 35%, and an isolation window of 3 u, an activation q = 0.25, and an activation time of 30 ms, was used. The conditions for MS3 and MS4 were the same, except that the thresholds were set to 300 and 100 counts, respectively.
Screening for V. cholerae Carbohydrate Recognition
In the initial screening for carbohydrate recognition by V. cholerae, mixtures of glycosphingolipids from various sources were used in order to expose the bacteria to a large number of potentially binding-active carbohydrate structures. Thus, the binding of the bacteria to acid and non-acid glycosphingolipid mixtures isolated from the small intestine of different species (human, rat, cat, rabbit, dog, monkey and pig), erythrocytes of different species (human, cat, rabbit, dog, horse, chicken and sheep), human cancers (lung cancer, kidney cancer, colon cancer, liver cancer and gastric cancer), and rabbit thymus, was tested. In most non-acid glycosphingolipid fractions a binding of both the El Tor strain and the classical strain to compounds migrating in the mono- and diglycosylceramide regions was observed (exemplified in Figure 1, lanes 2, 7, 8, 10). In addition, a number of slow-migrating minor glycosphingolipids in non-acid fractions of human small intestine, dog erythrocytes, cod intestine, rabbit thymus, rabbit erythrocytes, and chicken erythrocytes were distinctly recognized by the El Tor strain (Figure 1, lanes 1–3, 8–10).
(A) Chemical detection by anisaldehyde. (B) Autoradiograms obtained by binding of V. cholerae JBK 70. The lanes were: Lane 1, non-acid glycosphingolipids of human small intestine, 20 µg; Lane 2, non-acid glycosphingolipids of dog erythrocytes, 20 µg; Lane 3, non-acid glycosphingolipids of cod intestine, 20 µg; Lane 4, A type 2 heptaosylceramide (GalNAcα3(Fucα2)Galβ4(Fucα3)GlcNAcβ3Galβ4Glcβ1Cer), 4 µg; Lane 5, globotriaosylceramide (Galα4Galβ4Glcβ1Cer), 4 µg; Lane 6, acid glycosphingolipids of human small intestine, 40 µg; Lane 7, non-acid glycosphingolipids of cat erythrocytes, 40 µg; Lane 8, non-acid glycosphingolipids of rabbit thymus, 40 µg; Lane 9, non-acid glycosphingolipids of rabbit erythrocytes, 40 µg; Lane 10, non-acid glycosphingolipids of chicken erythrocytes, 40 µg.
Characterization of the El Tor Binding Slow-migrating Glycosphingolipid of Human Small Intestine
After separation of the non-acid fraction from human small intestine, the subfractions obtained were pooled according to El Tor binding activity, giving a subfraction containing the slow-migrating El Tor binding compound (designated fraction HI-I).
Based on the observations listed below fraction HI-I was characterized as Galβ3GlcNAcβ4Galβ3(Fucα4)GlcNAcβ3Galβ4Glcβ1Cer, i.e. a Lea pentaosylceramide substituted with a terminal lacto moiety.
- On thin-layer chromatograms the El Tor binding glycosphingolipid migrated in the hexa−/heptaglycosylceramide region (Figure 1, lane 1; Figure 2A–B, lane 1).
- LC-ESI/MS of the oligosaccharides released from fraction HI-I by hydrolysis with endoglycoceramidase II gave two molecular ions (Figure 2D). The major [M-H+]− ion at m/z 1217 indicated an oligosaccharide with one Fuc, two HexNAc and four Hex, while the minor [M-H+]− ion at m/z 852 indicated an oligosaccharide with one Fuc, one HexNAc and three Hex. The MS2 spectrum of the [M-H+]− ion at m/z 852 was characteristic for the Lea pentasaccharide  (data not shown). MS2 of the [M-H+]− ion at m/z 1217 gave a C-type fragment ion series (C2α at m/z 382, C3α at m/z 544, C4α at m/z 893, and C5α at m/z 1055) demonstrating a Hex-HexNAc-Hex-(Fuc)HexNAc-Hex-Hex sequence (Figure 2E). Intense cross-ring 0,2A-type fragments are diagnostic for carbohydrates substituted at C-4 –. The 0,2A6 ion at m/z 1157, and the 0,2A6-H2O ion at m/z 1139, were derived from cross-ring cleavage of the 4-substituted Glc of the lactose unit at the reducing end. However, no 0,2A2 fragment ion at m/z 281 (not shown), or 0,2A4 fragment ion at m/z 646, were observed, suggesting that the HexNAcs were substituted at 3-position.
- 1H NMR of fraction HI-I shows a mixture of three compounds, all with Lea signatures (Figure 2F). The two major structures (∼35% and 60%, respectively) are Galβ3(Fucα4)GlcNAcβ4Galβ3(Fucα4)GlcNAcβ3Galβ4Glcβ1Cer previously characterized by NMR , and its precursor Galβ3GlcNAcβ4Galβ3(Fucα4)GlcNAcβ3Galβ4Glcβ1Cer in which the terminal Fucα4 is lacking resulting in a lacto terminal, as found above by mass spectrometry. This structural difference results in drastic shifts of the anomeric resonances arising from the lacto terminal relative to the same residues in the Lea terminal: downfield by +0.034 ppm for GlcNAcβ3 and upfield for Galβ3 by −0.169 ppm, thus yielding shifts corresponding to those seen for the lacto-terminated hexaosylceramide at 4.794 ppm and 4.14 ppm (Table 1). All other resonances of the lacto-terminated compound overlap completely with those of the dimeric Lea structure (Figure 2F; Table 1). The third minor compound (∼5%) is the Lea pentaosylceramide (Galβ3(Fucα4)GlcNAcβ3Galβ4Glcβ1Cer).
(A) Chemical detection by anisaldehyde. (B) Autoradiogram obtained by binding of V. cholerae strain JBK 70. (C) Autoradiogram obtained by binding of H. pylori strain J99. The lanes were: Lane 1, fraction HI-I from human small intestine, 4 µg; Lane 2, NeuAcα3Galβ4(Fucα3)GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glcβ1Cer (sialyl-Lex-octaosylceramide), 4 µg; Lane 3, Galβ4(Fucα3)GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glcβ1Cer (Lex-heptaosylceramide), 4 µg; Lane 4, NeuAcα3Galβ4GlcNAcβ3Galβ4(Fucα3)GlcNAcβ3Galβ4Glcβ1Cer (VIM2), 4 µg; Lane 5, Galβ4GlcNAcβ3Galβ4(Fucα3)GlcNAcβ3Galβ4Glcβ1Cer (de-sialo VIM2), 4 µg; Lane 6, NeuAcα3Galβ4(Fucα3)GlcNAcβ3Galβ4(Fucα3)GlcNAcβ3Galβ4Glcβ1Cer (sialyl-dimeric-Lex), 4 µg; Lane 7, Galβ4(Fucα3)GlcNAcβ3Galβ4(Fucα3)GlcNAcβ3Galβ4Glcβ1Cer (dimeric-Lex), 4 µg. The sialic acid binding H. pylori strain J99 (C) was used as a control for removal of sialic acid from the compounds in lanes 3, 5 and 7 . (D) Base peak chromatogram from LC-ESI/MS of the oligosaccharides derived from fraction HI-I by hydrolysis with Rhodococcus endoglycoceramidase II. (E) MS2 spectrum of the [M-H+]− ion at m/z 1217 (retention time 22.9 min). (F) Anomeric region of the 600 MHz 1H NMR spectrum of fraction HI-I (30°C). The designations G and H refer to Table 1.
Characterization of the El Tor Binding Slow-migrating Glycosphingolipids of Rabbit Thymus
After separation of the non-acid glycosphingolipid fraction from rabbit thymus, and pooling of the subfractions obtained according El Tor binding activity, four El Tor binding fractions were obtained, designated fraction TH-I, TH-II, TH-III, and TH-IV, respectively. These fractions were characterized by mass spectrometry, 1H NMR, and binding of the Galβ4GlcNAcβ-binding E. cristagalli lectin , as follows.
A. Fraction TH-I.
Fraction TH-I was characterized as GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glcβ1Cer. This conclusion is based on the following observations:
- On thin-layer chromatograms fraction TH-I migrated in the pentaglycosylceramide region (Figure 3B, lane 2).
- No binding of the E. cristagalli lectin to fraction TH-I was obtained (Figure 3C, lane 2).
- LC-ESI/MS of the oligosaccharides obtained by hydrolysis of fraction TH-1 gave a major [M-H+]− ion at m/z 909, demonstrating an oligosaccharide with two HexNAc and three Hex (Figure 4A). MS2 of the ion at m/z 909 resulted in a series of C-type fragment ions (C2 at m/z 382, C3 at m/z 585, and C4 at m/z 747) identifying a pentasaccharide with HexNAc-Hex-HexNAc-Hex-Hex sequence (Figure 4B). The 0,2A3 fragment ion at m/z 484, and the 0,2A3-H2O fragment ion at m/z 466, indicated a 4-substituted internal HexNAc. The 0,2A5 ion at m/z 849, and the 0,2A5-H2O ion at m/z 831, were derived from cross-ring cleavage of the 4-substituted Glc of the lactose part.
- The proton NMR spectrum of fraction TH-1 in Figure 4C reveals a single species showing two GlcNAcβ3 residues at 4.603 ppm and 4.641 ppm, two overlapping Galβ4 residues at 4.25 ppm and a Glcβ1 at 4.15 ppm, consistent with the structure GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glcβ1Cer  (Table 1).
(A and D) Chemical detection by anisaldehyde. (B and E) Autoradiograms obtained by binding of V. cholerae JBK 70. (C and F) Autoradiograms obtained by binding of E. cristagalli lectin. The lanes on A–C were: Lane 1, neolactotetraosylceramide (Galβ4GlcNAcβ3Galβ4Glcβ1Cer), 4 µg; Lane 2, fraction TH-I isolated from rabbit thymus, 1 µg; Lane 3, fraction TH-II from rabbit thymus, 1 µg; Lane 4, Lane 4, sialylneolactohexaosylceramide (NeuGcα3Galβ4GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glcβ1Cer), 1 µg. The lanes on D–F were: Lane 1, total non-acid glycosphingolipids of rabbit thymus, 40 µg; B5 pentaosylceramide (Galα3Galβ4GlcNAcβ3Galβ4Glcβ1Cer), 4 µg; Lanes 3 and 4, subfractions isolated from rabbit thymus, 1 µg/lane; Lane 5, fraction TH-II from rabbit thymus, 1 µg; Lane 6, fraction TH-III, 1 µg; Lane 7, fraction TH-IV, 1 µg.
(A) Base peak chromatogram from LC-ESI/MS of the oligosaccharide obtained by digestion of fraction TH-I with endoglycoceramidase II. (B) MS2 spectrum of the [M-H+]− ion at m/z 909 (retention time 32.2 min). (C) Anomeric region of the 600 MHz 1H NMR spectrum of fraction TH-I (30°C). The designation B refer to Table 1.
B. Fraction TH-II.
Characterization of the El Tor binding fraction TH-II demonstrated neolactohexaosylceramide (Galβ4GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glcβ1Cer) as the major compound. This conclusion was based on the following properties:
- On thin-layer chromatograms fraction TH-II migrated in the hexaglycosylceramide region (Figure 3A–B, lane 3; Figure 3D–E, lane 5).
- Fraction TH-II was recognized by the E. cristagalli lectin (Figure 3C, lane 3; Figure 3F, lane 5).
- LC-ESI/MS of the oligosaccharides obtained by hydrolysis of fraction TH-II gave a major [M-H+]− ion at m/z 1071, demonstrating an oligosaccharide with two HexNAc and four Hex, and a minor [M-H+]− ion at m/z 868, indicating an oligosaccharide with one HexNAc and four Hex (data not shown). MS2 of the major [M-H+]− ion at m/z 1071 gave a series of prominent C-type fragment ions (C2 at m/z 382, C3 at m/z 544, C4 at m/z 747, and C5 at m/z 909) identifying a hexasaccharide with Hex-HexNAc-Hex-HexNAc-Hex-Hex sequence (Figure S1A). A 4-substitution of the internal HexNAc was identified by the 0,2A3 fragment ion at m/z 646, and the 0,2A3-H2O fragment ion at m/z 628.
- The 1H NMR spectrum of fraction TH-II (not shown) reveals a mixture of neolactohexaosylceramide, as evidenced by two overlapping GlcNAcβ3 resonances at 5.643 ppm and two overlapping Galβ4 resonances at 4,25 ppm, as well as a third one at 4.20 ppm, and a Glcβ1 resonance at 4.16 ppm yielding Galβ4GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glcβ1Cer , and the B5 pentaosylceramide, as seen by resonances at 4.826 ppm (Galα3), 4.627 ppm (GlcNAcβ3) and 4.279 ppm (penultimate Galβ4), which together with the lactosylceramide part yields Galα3Galβ4GlcNAcβ3Galβ4Glcβ1Cer  (Table 1).
C. Fraction TH-III.
The main compound of fraction TH-III was a neolactoheptaosylceramide with a terminal Galili epitope (Galα3Galβ4GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glcβ1Cer). This conclusion was based on the following observations:
- Fraction TH-III migrated in the heptaglycosylceramide region on thin-layer chromatograms (Figure 3D–E, lane 6).
- Fraction TH-III was not recognized the E. cristagalli lectin (Figure 3F, lane 6).
- LC-ESI/MS of the oligosaccharides released from fraction TH-III gave a major [M-H+]− ion at m/z 1233, demonstrating an oligosaccharide with two HexNAc and five Hex (data not shown). A heptasaccharide with Hex-Hex-HexNAc-Hex-HexNAc-Hex-Hex sequence was identified by the series of C-type fragment ions (C3 at m/z 544, C4 at m/z 747, C5 at m/z 909, and C5 at m/z 1071) obtained by MS2 of the ion at m/z 1233 (Figure S1B). A terminal Hex-Hex-HexNAc sequence with a substitution at C-4 of the HexNAc was identified by the 0,2A3 fragment ion at m/z 443, and the 0,2A3-H2O fragment ion at m/z 425. In the same manner, the 0,2A5 fragment ion at m/z 808, and the 0,2A5-H2O fragment ion at m/z 790, indicated a 4-substitution of the internal HexNAc, while the 0,2A7 ion at m/z 1173, and the 0,2A7-H2O ion at m/z 1155, were obtained by cross-ring cleavage of the 4-substituted Glc of the lactose unit at the reducing end.
- The 1H NMR spectrum of fraction TH-III (not shown) shows a structure having a Galili terminus elongated by an internal Galβ4GlcNAcβ3 yielding Galα3Galβ4GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glcβ1Cer, having almost identical shift values as the B5 pentaosylceramide (Table 1). A minor second species is also present, seen by a GlcNAcβ3 doublet at 4.794 ppm and a Galβ3 doublet at 4.13 ppm, which indicate a lacto-terminated hexaglycosylceramide Galβ3GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glcβ1Cer , where the remaining anomeric signals strongly overlap with those of the other structure (Table 1).
D. Fraction TH-IV.
El Tor binding fraction TH-IV was characterized as neolactooctaosylceramide (Galβ4GlcNAcβ3Galβ4GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glcβ1Cer). This conclusion was based on the following properties:
- On thin-layer chromatograms fraction TH-IV migrated in the octaglycosylceramide region (Figure 3D–E, lane 7).
- The E. cristagalli lectin bound to fraction TH-IV (Figure 3F, lane 7).
- LC-ESI/MS of the oligosaccharides released from fraction TH-IV gave a minor [M-H+]− ion at m/z 1233 as above, and a major [M-H+]− ion at m/z 1436, demonstrating an oligosaccharide with three HexNAc and five Hex (data not shown). MS2 of the ion at m/z 1436 gave a series of C-type fragment ions (C3 at m/z 544, C4 at m/z 747, C5 at m/z 909, C6 at m/z 1112, and C7 at m/z 1274) indicating an octasaccharide with Hex-HexNAc-Hex-HexNAc-Hex-HexNAc-Hex-HexNAc-Hex-Hex sequence (Figure S1C). As above, the 0,2A cross-ring cleavage ions (0,2A4 at m/z 646 and 0,2A6 at m/z 1011) identified substitutions at C-4 of the internal HexNAcs, while the 0,2A8 ion at m/z 1376 was obtained by cross-ring cleavage of the 4-substituted Glc of the lactose unit.
- The 1H NMR spectrum of TH-IV (Figure S1D) is very similar to TH-II revealing a neolactoocta compound and the seven-sugar Galili-terminated structure described above. The chemical shifts are thus identical to those described for fraction TH-II (Table 1).
Thus, the slow-migrating El Tor binding glycosphingolipids of rabbit thymus were characterized as GlcNAcβ3-neolactotetraosylceramide, neolactohexaosylceramide, the B7 heptaosylceramide and neolactooctaosylceramide. In fraction TH-II, the B5 pentaosylceramide was also present, but this compound was not recognized by of V. cholerae (see below).
The El Tor binding glycosphingolipids of cod intestine (Figure 1, lane 3) and chicken erythrocytes (Figure 1, lane 10) were also identified as neolactohexaosylceramide by the same set of methods (data not shown). Furthermore, the uppermost El Tor binding glycosphingolipid of dog erythrocytes (Figure 1, lane 2) was characterized as Galα3Galβ4GlcNAcβ6(Galα3Galβ4GlcNAcβ3)Galβ4GlcNAcβ3Galβ4Glcβ1Cer (Figure S2), i.e. a branched neolactodecaglycosylceramide with terminal Galili epitopes.
Thus, lacto or neolacto sequences were present in all complex El Tor binding glycosphingolipids hitherto characterized. In addition, neolactotetraosylceramide (Galβ4GlcNAcβ3Galβ4Glcβ1Cer) and lactotetraosylceramide (Galβ3GlcNAcβ3Galβ4Glcβ1Cer) were occasionally recognized. Binding to neolactotetraosylceramide and lactotetraosylceramide required 4 µg of the compound on the thin-layer plate, and was only found in approximately 70% of the experiments, indicating that the binding epitope was not optimally exposed in these relatively short glycosphingolipids. For neolactotetraosylceramide an increased binding is obtained by the addition of a terminal GlcNAcβ3 (No. 2), and inspection of the El Tor binding compounds in Figure 5 reveals that the GlcNAcβ3Galβ3/4GlcNAc sequence is the common denominator of all the El Tor binding lacto/neolacto based glycosphingolipids with more than four carbohydrate units.
The red circles marks the GlcNAcβ3Galβ3/4GlcNAc sequence required for high affinity binding. Blue ring, glucose; yellow ring, galactose; blue square, N-acetylglucosamine; red triangle, fucose. Binding is defined as follows:+++denotes a binding when 1 µg of the glycosphingolipid was applied on the thin-layer chromatogram, while+denotes an occasional binding at 4 µg.
Characterization of a Novel Glycosphingolipid of Rabbit Erythrocytes Recognized by V. cholerae
The non-acid glycosphingolipids of rabbit erythrocytes has been characterized in detail by mass spectrometry and NMR, demonstrating a number of Galα3Galβ4GlcNAc-terminated linear and di−/triantennary oligo- and polyglycosylceramides –. The major non-acid glycosphingolipid of rabbit erythrocytes is the B5 pentaosylceramide, and the El Tor binding glycosphingolipid was found just below this compound (Figure 1B, lane 9; Figure 6B, lane 4). Separation of a non-acid glycosphingolipid fraction from rabbit erythrocytes and pooling of the subfractions obtained according to El Tor binding activity gave a fraction containing the El Tor binding compound (designated fraction RE-I). Structural characterization by mass spectrometry and proton NMR, as detailed below, identified two glycosphingolipids in fraction RE-I; the B5 pentaosylceramide and a novel glycosphingolipid structure, Galα3Galα3Galβ4GlcNAcβ3Galβ4Glcβ1Cer, i.e a B5 pentaglycosylceramide elongated by Galα3.
(A) Chemical detection by anisaldehyde. (B) Autoradiogram obtained by binding of V. cholerae strain JBK 70. The lanes were: Lane 1, total non-acid glycosphingolipids of rabbit thymus, 40 µg; Lane 1, total non-acid glycosphingolipids of rabbit erythrocytes, 40 µg; Lane 3, B5 pentaosylceramide (Galα3Galβ4GlcNAcβ3Galβ4Glcβ1Cer), 4 µg; Lane 4, fraction RE-I of rabbit erythrocytes, 2 µg. (C) Base peak chromatogram from LC-ESI/MS of the oligosaccharides derived from fraction RE-I by hydrolysis with endoglycoceramidase II. (D) MS2 spectrum of the [M-H+]− ion at m/z 868 (retention time 14.0 min). (E) MS2 spectrum of the [M-H+]− ion at m/z 1030 (retention time 14.7 min). (F) Anomeric region of the 600 MHz 1H NMR spectrum of fraction RE-I (30oC). The designations A and D refer to Table 1.
- LC-ESI/MS of the oligosaccharides obtained by hydrolysis of fraction RE-1 gave a major [M-H+]− ion at m/z 868, demonstrating an oligosaccharide with one HexNAc and four Hex, and a [M-H]− ion at m/z 1030, indicating an oligosaccharide with one HexNAc and four Hex (Figure 6C).
The MS2 spectrum of the [M-H+]− ion at m/z 868 had a C-type fragment ion series (C2 at m/z 341, C3 at m/z 544, and C4 at m/z 706), identifying a Hex-Hex-HexNAc-Hex-Hex sequence (Figure 6D). The 0.2A3 fragment ion at m/z 443, and the 0,2A3-H2O fragment ion at m/z 425, demonstrated a 4-substitution of the HexNAc, i.e. a type 2 core chain, while the 0.2A5 fragment ion at m/z 808, and the 0,2A5-H2O fragment ion at m/z 790, were obtained by cross-ring cleavage of the 4-substituted Glc of the lactose unit.
MS2 of the [M-H+]− ion at m/z 1030 (Figure 6E) gave a series of C-type fragment ions (C2 at m/z 341, C3 at m/z 503, C4 at m/z 706, and C5 at m/z 868) indicating a Hex-Hex-Hex-HexNAc-Hex-Hex sequence. A type 2 core chain was demonstrated by the 0,2A4 fragment ion at m/z 605, and the 0,2A4-H2O fragment ion at m/z 587, which are diagnostic for substitution of the HexNAc at C4.
- 1H NMR of fraction RE-1 reveals a mixture of one major component and several minor ones. The main structure was identified as the B5 pentaosylceramide (Galα3Galβ4GlcNAcβ3Galβ4Glcβ1Cer) from signals at 4.826 ppm (Galα3), 4.65 ppm (GlcNAcβ3), 4.279 ppm (penultimate Galβ4), 4.25 ppm (Galβ4) and 4.16 ppm (Glcβ1) (Figure 6F). The spectrum also had three α-signals clustered around 4.90 ppm, one of which coincides precisely with an internal Galα3 (4.893 ppm) substituted with a terminal Galα3, as found earlier for Galα3Galα3Galβ4Glcβ1Cer . Thus, a structure with the sequence Galα3Galα3Galβ4GlcNAcβ3Galβ4Glcβ1Cer is present, in accord with the mass spectrometry data above, and where the shifts for the remaining sugar residues are very close to those found for the B5 pentaosylceramide (Table 1). The two α-signals seen on either side of the internal Galα3 resonance just mentioned could not be assigned.
Binding to Reference Glycosphingolipids
Finally, the binding of the El Tor strain to a number of reference glycosphingolipids was evaluated (summarized in Table 2). Like many other bacteria, the El Tor strain bound to lactosylceramide with phytosphingosine and hydroxy fatty acids (No. 32 in Table 2; Figure 7B, lane 4, upper band), isoglobotriaosylceramide (No. 33; Figure 7B, lane 5, upper band), galactosylceramide (No. 31; Figure 7B, lane 1, upper band), gangliotriaosylceramide (No. 35; Figure 7B, lane 1, lower band), and gangliotetraosylceramide (No. 36). However, the majority of the tested compounds, as globoseries glycosphingolipids (Nos. 42-44 in Table 2), blood group A/B/H-terminated glycosphingolipids (Nos. 7, 11, 12, 14, 23, 26 and 28), Lex-, Lea- and Leb-terminated glycosphingolipids (Nos. 8, 16, 17, 27, 29 and 30), and the gangliosides (Nos. 18–23 and 46–49), were not recognized by the bacteria.
(A, C, E) Chemical detection by anisaldehyde. (B, D, G) Autoradiograms obtained by binding of El Tor V. cholerae strain JBK 70. (F) Autoradiogram obtained by binding of classical V. cholerae strain CVD103. (H) Autoradiogram obtained by binding of El Tor GbpA deletion mutant strain 1382. The lanes on (A and B) were: Lane 1, galactosylceramide (Galβ1Cer), 4 µg, and gangliotriaosylceramide (GalNAcβ4Galβ4Glcβ1Cer), 4 µg; Lane 2, glucosylceramide (Glcβ1Cer), 4 µg, and globotriaosylceramide (Galα4Galββ4Glcβ1Cer), 4 µg; Lane 3, lactosylceramide (Galβ4Glcβ1Cer) with sphingosine and non-hydroxy fatty acids, 4 µg, and Forssman pentaosylceramide (GalNAcα3GalNAcβ3Galα4Galβ4Glcβ1Cer), 4 µg; Lane 4, lactosylceramide (Galβ4Glcβ1Cer) with phytosphingosine and hydroxy fatty acids, 4 µg, and globotetraosylceramide (GalNAcβ3Galα4Galβ4Glcβ1Cer), 4 µg; Lane 5, isoglobotriaosylceramide (Galα3Galβ4Glcβ1Cer), 4 µg, and Lea pentaosylceramide (Galβ3(Fucα4)GlcNAcβ3Galβ4Glcβ1Cer), 4 µg; Lane 6, Lex pentaosylceramide (Galβ4(Fucα3)GlcNAcβ3Galβ4Glcβ1Cer), 4 µg; lane 7, H type 1 pentaosylceramide (Fucα2Galβ3GlcNAcβ3Galβ4Glcβ1Cer), 4 µg. The lanes on (C and D) were: Lane 1, B5 pentaosylceramide (Galα3Galβ4GlcNAcβ3Galβ4Glcβ1Cer), 4 µg; Lane 2, non-acid glycosphingolipids of rabbit erythrocytes, 40 µg; Lane 3, Galα3Galα3Galβ4Glcβ1Cer, 1 µg; Lane 4, B type 2 hexaosylceramide (Galα3(Fucα2)Galβ4GlcNAcβ3Galβ4Glcβ1Cer), 4 µg. The lanes on (E-H) were: Lane 1, non-acid glycosphingolipids of rabbit thymus, 20 µg; Lane 2, lactotetraosylceramide (Galβ3GlcNAcβ3Galβ4Glcβ1Cer), 2 µg; Lane 3, Galα3Galα3Galβ4Glcβ1Cer, 4 µg; Lane 4, B5 pentaosylceramide, 4 µg; Lane 5, gangliotriaosylceramide, 4 µg; Lane 6, H type 1 pentaosylceramide, 4 µg.
When reference glycosphingolipids with terminal α3-linked Gal were examined for V. cholerae El Tor binding activity (Figure 7D) the bacteria bound to Galα3Galα3Galβ4Glcβ1Cer (lane 3), in addition to the Galα3Galα3Galβ4GlcNAcβ3Galβ4Glcβ1Cer from rabbit erythrocytes (lane 2). Again, no binding to the B5 pentaosylceramide (lane 1) occurred, and the B type 2 hexaosylceramide (Galα3(Fucα2)Galβ4GlcNAcβ3Galβ4Glcβ1Cer; lane 4) was also non-binding.
Glycosphingolipid Binding by Classical V. cholerae
The glycosphingolipid binding of the El Tor strain and the classical strain were tested in parallel throughout the experiments described above. Although the classical strain often gave a high background, the glycosphingolipid binding pattern of the classical strain (Figure 7F) correlated well with the glycosphingolipid recognition by the El Tor strain. Thus, the classical strain also bound to neolacto- (lane 1), lacto- (lane 2), and Galα3Galα3Gal-terminated glycosphingolipids (lane 3), and also to lactosylceramide with hydroxy ceramide (not shown) and gangliotriaosylceramide (lane 5). The only exception was the El Tor binding Galβ3GlcNAcβ4Galβ3(Fucα4)GlcNAcβ3Galβ4Glcβ1Cer of human small intestine, which was not recognized by the classical strain (data not shown).
Glycosphingolipid Recognition by a GbpA Deletion Mutant Strain
Thereafter, the role of the chitin binding protein GbpA in the binding of V. cholerae to glycosphingolipids was investigated by using a strain with inactivation of the gbpA gene. This strain displayed the same binding activities as the parental strain, i.e. lacto/neolacto glycosphingolipids, Galα3Galα3Gal-terminated glycosphingolipids and lactosylceramide/gangliotriaosylceramide were still recognized (exemplified in Figure 7H).
Pretreatment of Bacterial Cells
In order to investigate the bacterial structures involved in V. cholerae glycosphingolipid binding the bacterial cells were subjected to pretreatments prior to the chromatogram bindng assays. Trypsin treatment and heating of the bacteria each decreased the binding to neolacto, lacto, Galα3Galα3Gal-terminated, and lactosylceramide-related glycosphingolipids (Figure S3).
Adhesion of microbes to target cells is considered to be an important step in the infection process, allowing an efficient delivery of toxins and other virulence factors close to the cell surface. Potential host receptors, the majority of which are glycoconjugates, have been identified for a large number of bacteria , . However, little or no data exists regarding the potential adhesion strategies of V. cholerae bacterial cells. In this study, the carbohydrate binding specificity of one El Tor strain and one classical strain was examined by binding to a large number of variant glycosphingolipids. Thereby, highly specific interactions with a number of non-acid glycosphingolipids were obtained. We focused on minor complex El Tor binding compounds, which were isolated and characterized by mass spectrometry and 1H NMR. After comparison with the binding of the bacteria to a number of reference glycosphingolipids, three modes of El Tor glycosphingolipid recognition could be discerned.
I. Lacto/Neolacto Binding
Most of the V. cholerae binding glycosphingolipids isolated and characterized in this study have lacto or neolacto core chains (Figure 5, Table 2). Lacto and neolacto glycosphingolipids are present in the human small intestinal epithelium -, thus may contribute to V. cholerae colonization of the human small intestine. The V. cholerae binding lacto-Lea glycosphingolipid (Galβ3GlcNAcβ4Galβ3(Fucα4)GlcNAβ3Galβ4Glcβ1Cer) was isolated from human small intestine, and the binding of the Galβ4GlcNAc-binding E. cristagalli lectin to slow-migrating glycosphingolipids of human small intestine demonstrates the presence of complex unsubstituted neolacto glycosphingolipids in the human small intestine . In contrast, the N-linked glycans of human small intestinal glycoproteins have terminal neolacto chains which are heavily substituted with fucose , and thus should not be recognized by V. cholerae.
For high affinity binding to lacto/neolacto based glycosphingolipids a GlcNAcβ3Galβ3/4GlcNAc sequence is required (Figure 5). However, certain substitutions of the GlcNAcβ3Galβ3/4GlcNAc sequence abolished the binding of the bacteria. Thus, addition of a terminal NeuGcα3 to the terminal Gal of neolactohexaosylceramide (No. 3), yielding sialyl-neolactohexaosylceramide (No. 18), blocks the access to the binding epitope. The binding also disappears upon substitution of either of the GlcNAcs of neolactohexaosylceramide with a Fucα3 (Nos. 15 and 16). In the case of the Galβ3GlcNAcβ4Galβ3(Fucα4)GlcNAβ3Galβ4Glcβ1Cer from human small intestine (No. 25), the binding is abolished by substitution of the penultimate GlcNAc of with a Fuc in α3-position (No. 30).
The main difference between the lacto core and the neolacto core glycosphingolipids is that substitution of the innermost GlcNAc by a Fucα is tolerated for the compound with lacto core (No. 25), while it abolishes V. cholerae binding in the case of the glycosphingolipid with neolacto core (No. 15). This difference could be caused by conformational effects of the Fuc residues on the exposure of the binding epitope. It may also be that the lacto and neolacto sequences are recognized by two separate adhesins. The latter suggestion is somewhat supported by the fact that the classical strain fails to bind to the lacto terminated glycosphingolipid from human intestine, although it binds to the complex neolacto glycosphingolipids. Resolution of this issue must await the identification of the adhesin(s) involved in the lacto/neolacto binding.
There is a strong correlation between the severity of cholera and the blood group ABO phenotype of infected individuals, with blood group O individuals being more prone to develop severe diarrhea upon contracting V. cholerae infection than individuals with blood groups A, B or AB , . However, in this study no binding of V. cholerae bacterial cells to glycosphingolipids with terminal blood group A, B or H determinants was obtained. A tempting speculation is that the bacteria instead might benefit from the binding to lacto and neolacto core chains, since they thereby will avoid individual variations.
Recognition of glycoconjugates with neolacto sequences is a common theme in microbial carbohydrate binding, and has also been described for e.g. Streptococcus pneumoniae , Helicobacter pylori , and relapsing fever Borrelia . In each case the detailed structural requirements for binding differs. Interestingly, the V. cholerae binding GlcNAcβ3Galβ4GlcNAc sequence is also the minimum sequence recognized by H. pylori. However in contrast to V. cholerae, H. pylori binds to sialyl-neolactohexosylceramide, indicating a different architecture of the carbohydrate binding site of the neolacto binding adhesins.
In summary, glycosphingolipids with repetitive lacto or neolacto elements are preferentially recognized by V. cholerae. An increased binding is obtained by addition of a terminal β3-linked GlcNAc to neolactotetraosylceramide, indicating that the preferred binding epitope is the GlcNAcβ4Galβ4GlcNAc sequence. Since minute amounts of several of the binding-active glycosphingolipids were obtained the relative binding affinities could, unfortunately, not be accurately determined.
II. Galα3Galα3Gal Binding
The binding to Galα3Galα3Galβ4GlcNAcβ4Galβ4Glcβ1Cer and Galα3Galα3Galβ4Glcβ1Cer is the second binding specificity of V. cholerae. Since the bacteria do not recognize the B5 glycosphingolipid (Galα3Galβ4GlcNAcβ4Galβ4Glcβ1Cer), the minimum binding epitope in this case is the Galα3Galα3Gal moiety. This sequence could be the product of the α1,3-galactosyltransferase or the iGb3 synthase , . However, functional forms of these two enzymes have not been found in humans, and thus it is not likely that glycoconjugates with the Galα3Galα3Gal sequence have a role in the adhesion of V. cholerae to the human small intestine.
III. Lactosylceramide Binding
The third carbohydrate binding specificity of V. cholerae is represented by the binding to lactosylceramide, with a concomitant binding to galactosylceramide, isoglobotriaosylceramide, gangliotriaosylceramide and gangliotetraosylceramide. The binding in the mono-, di- and triglycosylceramide region in Figure 1, Figure 7F and 7H, and Figure S3B and S3D, is thus most likely due to recognition of these compounds. The same glycosphingolipid binding pattern has previously been reported for many other bacteria, both pathogens and members of the indigenous flora . While minute amounts of diglycosylceramides, most likely lactosylceramide, are present in the human intestinal epithelium , isoglobotriaosylceramide, gangliotri- and gangliotetraosylceramide have not been found in these cells and, thus, it is unlikely that these compounds are critical receptors for V. cholerae.
V. cholerae chitin binding protein GbpA has been proposed to mediate attachment of the bacteria to human intestine by binding to GlcNAc-containing glycoconjugates , . We hypothesized that GbpA was involved in the binding of V. cholerae to the lacto/neolacto containing glycosphingolipids. However, the lacto/neolacto binding of V. cholerae remained after inactivation of the gbpA gene. Thus, the binding of V. cholerae to chitin and to lacto/neolacto containing glycosphingolipids represents two separate binding specificities. This is supported by the glycan array data in a recent crystalization study of GbpA, where only binding to chitin oligosaccharides was observed . The GbpA deletion mutant strain also bound to the Galα3Galα3Gal-terminated compounds and the set of reference glycosphingolipid recognized by the wild type El Tor strain.
One important virulence factor of V. cholerae is the toxin-coregulated pilus (TCP). Expression of TCP is essential for V. cholerae colonization of the human small intestine , and it has been suggested that TCP mediates binding of the bacteria to the intestinal epithelium . The culture conditions mainly used in the present study (AKI medium at 30°C) favors TCP production . However, the binding-active glycosphingolipids were also recognized after culture in CFA broth at 27°C, i.e. under conditions when little or no TCP is expressed, indicating that the glycosphingolipid binding capacity of V. cholerae does not reside in the toxin-coregulated pilus.
The glycosphingolipid binding was decreased by treatment of the bacteria with trypsin and heat, indicating that bacterial cell surface proteins were involved in the interaction with carbohydrates. However, Schild et al. have reported that the attachment of lipopolysaccharide and capsule mutants of V. cholerae to the brush borders of the mucus-producing human intestinal cell line HT29-Rev MTX is reduced, demonstrating that lipopolysaccharides are important for V. cholerae adherence . In order to investigate the role of lipopolysaccharides in V. cholerae glycosphingolipid binding, the bacterial cells were incubated with polyclonal antibodies to V. cholerae O1 antigen prior to the chromatogram binding assays. Occasionally a reduced binding to some glycosphingolipids was thereby obtained (data not shown), but the patterns were not consistent. One possible explanation for this variable reduction is that the coating of the bacterial cell surface with antibodies causes a general steric hindrance of cell surface components and adhesins that promote glycosphingolipid binding.
In this study we have for the first time defined the carbohydrate binding properties of V. cholerae, which is important for understanding the molecular interactions between the bacteria and its target host cells. The next step is identification, isolation and molecular cloning of the adhesins involved in the different carbohydrate binding specificities, in order to evaluate their roles in the infection process.
Characterization of the El Tor Vibrio cholerae binding glycosphingolipid fractions TH-II, TH-III and TH-IV from rabbit thymus. (A) MS2 of the [M-H+]− ion at m/z 1071 (retention time 26.6 min) from LC-ESI/MS of the oligosaccharides derived from fraction TH-II. The interpretation formula shows the deduced oligosaccharide sequence. (B) MS2 of the [M-H+]− ion at m/z 1233 (retention time 27.8 min) from LC-ESI/MS of the oligosaccharides derived from fraction TH-III. The interpretation formula shows the deduced oligosaccharide sequence. (C) MS2 of the [M-H+]− ion at m/z 1436 (retention time 29.9 min) from LC-ESI/MS of the oligosaccharides derived from fraction TH-IV. The interpretation formula shows the deduced oligosaccharide sequence. (D) Anomeric regions of the 600 MHz proton NMR spectrum of fraction TH-IV from rabbit thymus (30oC). The designations F and I refer to Table 1.
Characterization of the El Tor binding slow-migrating glycosphingolipid of dog erythrocytes. (A) Chemical detection by anisaldehyde. (B) Autoradiogram obtained by binding of V. cholerae strain JBK 70. The lanes were: Lane 1, B5 pentaosylceramide (Galα3Galβ4GlcNAcβ3Galβ4Glcβ1Cer), 4 µg; Lane 2, fraction DE-I from dog erythrocytes, 4 µg; Lane 3, Galβ4GlcNAcβ3Galβ4GlcNAcβ3Galβ4Glcβ1Cer (neolactohexaglycosylceramide), 4 µg. (C) MS2 of the [M-2H+]2− ion at m/z 880 from LC-ESI/MS of the oligosaccharide derived from the El Tor binding fraction DE-I from dog erythrocytes by hydrolysis with Rhodococcus endoglycoceramidase. LC-ESI/MS of the oligosaccharides obtained by hydrolysis of fraction DE-I gave a major [M-2H+]2− ion at m/z 880, corresponding to a [M-H+]− ion at m/z 1760, indicating a decasaccharide with three HexNAc and seven Hex. The lower mass region of the MS2 spectrum was weak, but had a C3 ion at m/z 544, demonstrating a terminal with two Hex and one HexNAc. In addition, C type ions at m/z 1436 and m/z 1598 were present. (D) MS3 of the fragment ion at m/z 1436 gave a C type ion at m/z 1233, but no further information. (E) MS4 of the 0,2A5 fragment ion at m/z 1335 gave a 0,2A3 ion at m/z 443, demonstrating a terminal Hex-Hex-HexNAc sequence with 4-substitution of the HexNAc. The ion at m/z 688 was interpreted as C4/Z4α and C4/Z4β ions. Thus, the MS2 and MS4 spectral features suggested a branched decasaccharide with a terminal Hex-Hex-HexNAc, i.e. a Hex-Hex-HexNAc-(Hex-Hex-HexNAc-)Hex-HexNAc-Hex-Hex saccharide. The interpretation formula at the top shows the deduced oligosaccharide sequence. (F) Anomeric region of the 600 MHz proton NMR spectrum of the El Tor binding glycosphingolipid of dog erythrocytes (30°C). The designation J refers to Table 1. The 1H NMR spectrum reveals an essentially pure compound, which is characterized by two overlapping Galα3 resonances at 4.827 ppm, two GlcNAcβ3 at 4.652 ppm, one GlcNAcβ6 at 4.395 ppm, three Galβ4 around 4.28 ppm, a fourth Galβ4 at 4.254 ppm and lastly Glcβ1 at 4.161 ppm (Table 1). These data are practically identical to previously published findings for the branched Galα3Galβ4GlcNAcβ6(Galα3Galβ4GlcNAcβ3)Galβ4GlcNAcβ3Galβ4Glcβ1Cer allowing for temperature-induced differences .
Effects on glycosphingolipid binding by pretreatments of El Tor Vibrio cholerae bacterial cells. Radiolabeled V. cholerae strain JBK 70 was incubated at 37°C for 60 min, incubated at 65°C for 60 min, or treated with trypsin at 37°C for 60 min. Thereafter the suspensions were utilized in the chromatogram binding assay. (A) Chemical detection by anisaldehyde. (B, D) Autoradiograms obtained by binding of El Tor V. cholerae strain JBK 70 incubated at 37°C. (C) Autoradiogram obtained by binding of El Tor V. cholerae strain JBK 70 treated with trypsin at 37°C. (E) Autoradiogram obtained by binding of El Tor V. cholerae strain JBK 70 incubated at 65°C. The lanes were: Lane 1, non-acid glycosphingolipids of rabbit thymus, 40 µg; Lane 2, lactotetraosylceramide (Galβ3GlcNAcβ3Galβ4Glcβ1Cer), 2 µg; Lane 3, Galα3Galα3Galβ4Glcβ1Cer, 2 µg; Lane 4, isoglobotriaosylceramide (Galα3Galβ4Glcβ1Cer), 4 µg. The autoradiograms displayed in B and C were from one set of experiments run in parallel, and the autoradiograms displayed in D and E were from another set of experiments run in parallel.
The use of the LTQ linear quadrupole ion trap mass spectrometer (obtained by a grant from the Swedish Research Council (No. 342-2004-4434) to Gunnar Hansson), and the Varian 600 MHz NMR spectrometer at the Swedish NMR Centre, Hasselblad Laboratory, Göteborg University, are gratefully acknowledged.
Conceived and designed the experiments: ST HK JÅ SL ML. Performed the experiments: JB JÅ OT. Analyzed the data: JB JÅ HK OT SL ML ST. Contributed reagents/materials/analysis tools: SL ML ST. Wrote the paper: JB JÅ ML ST.
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