Human papillomavirus (HPV)-associated oropharyngeal squamous cell carcinoma is becoming more common. We examined prevalence and risk factors for oral HPV among men who have sex with men (MSM) and compared sampling and transport methods.
In 2010, 500 MSM (249 HIV-positive) attending Melbourne Sexual Health Centre answered a questionnaire, swabbed their mouth and throat and collected a gargled oral rinse sample. Half the oral rinse was transported absorbed in a tampon (to enable postage). HPV was detected by polymerase chain reaction, and genotyped by Roche Linear Array®. Men with HPV 16 or 18 were retested after six months.
Any HPV genotype was detected in 19% (95% confidence intervals (CI) 15–25%) of HIV-infected men and 7% (95% CI 4–11%) of HIV-negative men (p<0.001), and HPV 16 was detected in 4.4% (95% CI 2–8%) of HIV-infected men and 0.8% (0.1–2.8%) of HIV-negative men. Oral HPV was associated with: current smoking (adjusted odds ratio (aOR) 2.2 (95%CI: 1.2–3.9)), time since tooth-brushing (aOR per hour 0.87, 95%CI: 0.8–0.96) and number of lifetime tongue-kissing partners aOR 3.2 95%CI: (1.2–8.4) for 26–100 partners and 4.9 95%CI: (1.9–12.5) for>100 partners. Lifetime oral-penile sex partner numbers were significantly associated in a separate model: aOR 2.8(1.2–6.3) for 26–100 partners and 3.2(1.4–7.2) for>100 partners. HPV 16 and 18 persisted in 10 of 12 men after a median six months. Sensitivities of sampling methods compared to all methods combined were: oral rinse 97%, tampon-absorbed oral rinse 69%, swab 32%.
Oral HPV was associated with HIV infection, smoking, recent tooth-brushing, and more lifetime tongue-kissing and oral sex partners. The liquid oral rinse sample was more sensitive than a tampon-absorbed oral rinse or a self-collected swab.
Citation: Read TRH, Hocking JS, Vodstrcil LA, Tabrizi SN, McCullough MJ, et al. (2012) Oral Human Papillomavirus in Men Having Sex with Men: Risk-Factors and Sampling. PLoS One 7(11): e49324. doi:10.1371/journal.pone.0049324
Editor: Patrick S. Sullivan, Rollins School of Public Health, Emory University, United States of America
Received: June 18, 2012; Accepted: October 9, 2012; Published: November 16, 2012
Copyright: © 2012 Read et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This study was funded by Program Grant number 568971 of the National Health and Medical Research Council of Australia. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: CKF, JSH and AEG have received honoraria from CSL Biotherapies. CKF and AEG have received research funding from CSL Biotherapies. CKF owns shares in CSL Biotherapies, the manufacturer of Gardasil, and has received honoraria from Merck. SMG has received advisory board fees and grant support from Commonwealth Serum Laboratories and GlaxoSmithKline, and lecture fees from Merck and Sanofi Pasteur MSD. In addition, she has received funding through her institutions to conduct HPV vaccine studies for GlaxoSmithKline and for Merck. AEG has received honoraria and travel funding from Merck, and sits on the Australian advisory board for the Gardasil vaccine. TRHR is a site investigator on a Merck-sponsored study. MYC, SNT, MJM and CSB, have no conflicts of interest. This does not alter the authors’ adherence to all the PLOS ONE policies on sharing data and materials.
Oncogenic human papillomavirus (HPV), principally genotype 16, is now a recognised cause of a substantial proportion of oropharyngeal squamous cell carcinoma (SCC) and this proportion appears to be increasing. , ,  In an Australian cohort, the proportion of HPV-positive oropharyngeal SCC increased from 19% in 1987–1990 to 60% in 2005–6.  Over the same period the incidence of non HPV-associated head and neck cancers has been falling. , .
This increase in HPV-associated oropharyngeal SCC has led to a search for predictors of oral HPV infection in individuals without cancer. Multiple studies indicate that the presence of oral HPV DNA is associated with higher numbers of sexual partners, smoking and HIV infection , , , , . However it is unclear whether the risk of HPV infection is determined by the number of sexual partners in one’s lifetime or over a more recent period, and which specific sexual practice carries the greatest risk of infection. It is also unknown how frequently infection occurs after exposure, how long it persists and what determines the duration of infection. In addition, no data are available on the effect of eating, drinking and tooth-brushing on HPV detection.
To better understand the epidemiology of oral HPV infection population-based studies are needed. Population-based studies would be facilitated by self-collected oral samples that could be sent by post to laboratories. Gargled oral rinses have been reported as more sensitive samples for HPV DNA detection than tonsillar and oral mucosal brushings, ,  but large-scale postal surveys involving liquid samples are impractical due to postal regulations in some countries .
We performed a study with two aims. The first was to compare HPV detection from three sampling and transport methods: oral rinse samples, oral rinse samples absorbed in a tampon and a self-collected mouth and throat-mouth swab. The latter two can be mailed. We also assessed the impact of other factors that may affect HPV detection, such as eating, drinking and tooth-brushing.
Our second aim was to clarify the relative importance of lifetime compared to recent sexual partners and then look at different sexual practices in both time periods. We examined these questions in a population of men who have sex with men (MSM) attending a sexual health centre because they were likely to be at increased risk of oral HPV and do not seem to be protected by the HPV vaccination program which targets women. , 
This was a cross-sectional study of HIV-positive and HIV-negative MSM attending Melbourne Sexual Health Centre, Victoria, Australia, between 2 March 2010 and 17 June 2010. Methods for the two components of the study are described separately.
Risk Factor Study
In the clinic, participants completed a written questionnaire about risk factors including sexual history, smoking, alcohol-consumption and genital warts. The questionnaire also asked about factors that may affect detection, including how long prior to specimen collection they last ate, drank or brushed their teeth. The sexual history covered their number of sexual partners over different time periods (last two weeks, last 12 months, and lifetime) for tongue-kissing, oral-penile sex, and oral-anal sex. Each of these sexual practices was defined as involving only the participant’s mouth. Participants were also asked how many days since they had each type of sex, the proportion of lifetime male oral sex partners who ejaculated in their mouths, and the proportion who used condoms during oral sex with the study participant.
After providing written informed consent, participants were shown a video describing how to swab their own gums, mouth and throat with a flocked swab (Copan Diagnostic, Brescia, Italy). This was agitated in RNA stabilization reagent (RNAlater, Ambion Inc, Austin, USA) and transported to the laboratory for processing. Participants also gargled 20 ml saline for ten seconds and expelled it into a container (oral rinse sample). A researcher then divided the oral rinse sample into equal halves: one was absorbed by a tampon (Stayfree® Meds, Johnson & Johnson, Australia) while the other was left in the original container. In the laboratory, the tampon was squeezed firmly to retrieve as much absorbed saline as possible. The order of collecting the oral rinse and throat swab samples from participants was alternated weekly.
DNA was extracted using MagNA Pure LC (Roche Molecular Systems, Alameda, CA, USA). A 20 µl aliquot of extracted DNA was amplified in a PGMY09/11-based HPV consensus PCR assay , with a PCR-ELISA detection protocol.  All assays incorporated amplification of the β-globin gene as an internal control. All samples positive on the PGMY09/11 PCR test were genotyped using HPV Linear Array® (LA) Genotyping Test (Roche Molecular Systems), using 50 µl of extracted DNA, and following the manufacturer’s instructions with minor modifications as previously reported. ,  LA identifies 37 genotypes: 6, 11, 16, 18, 26, 31, 33, 35, 39, 40, 42, 45, 51, 52, 53, 54, 55, 56, 58, 59, 61, 62, 64, 66, 67, 68, 69, 70, 71, 72, 73, 81, 82 (previously known as IS39), 83, 84 and 89 (previously known as CP6108) .
Negative and positive controls were processed with each run, and lack of signal in the negative control was used to monitor possible carryover.
Men who had HPV 16 or 18 detected on any sample were referred for oral examination and retesting for oral HPV using a gargled oral rinse and a swab taken by an oral medicine specialist, six months later. A subset of 37 HIV positive men had anal swabs in a subsequent study of anal cancer screening, performed 16 months after this study. This study recruited from the same HIV-positive MSM population with the exception that only men aged ≥35 years were eligible. These swabs were tested for HPV by the same method and these data are included for comparison.
Sample sensitivity was calculated by comparing the number of positive specimens by each method to the total positive by any method and McNemar’s test was applied. HPV prevalence estimates and 95% confidence intervals (CI) were calculated using exact methods. Unadjusted and adjusted odds ratios and 95%CI were calculated to investigate associations with HPV by logistic regression using Stata 11.2 (Statacorp, College Station, Texas).
The questionnaire recorded number of sexual partners over three overlapping time intervals: the last 2 weeks, 12 months or lifetime. In order to select the most appropriate of these for the multivariate model we created three variables of non-overlapping time periods: variable 1) number of partners in the last 2 weeks; variable 2) number of partners in the last 12 months minus the number of partners in the last 2 weeks; and variable 3) number of partners over the lifetime minus the number of partners in the last 12 months. This was done for each of the sexual practices: tongue-kissing, oral-penile sex and oral-anal sex.
A separate model was generated for each sexual practice which included only the above three variables. This measured the association with oral HPV of each non-overlapping time period, adjusted for the other time periods.
Other risk-factors associated with HPV at P≤0.05 in the crude analysis were included in the adjusted models. Using a stepwise forward approach, variables were removed from the model as they became non-significant, with the exception of age. Because of the strong correlation between number of partners for oral-penile sex and tongue-kissing and the strong association of each with HPV, two separate logistic regression models are presented.
With an overall HPV prevalence of 13%, power of 80% and significance of 5%, a sample size of 500 would allow us to detect an odds ratio of 2.2 for a risk factor present in 30% of controls.
This research was approved by the Alfred Health Human Ethics Committee.
We recruited 500 MSM of whom 249 were HIV-positive and 251 HIV-negative. Of those approached to participate, 94% of HIV-positive men and 97% of HIV negative men agreed to participate. The median age of participants was 37 years, intraquartile range (IQR 27–45 years).
Sixty five men (13%; 95%CI: 10–16%) had at least one HPV type: 13 (3%; 95%CI: 1–4%) had HPV 16, 2 (0.4%; 95%CI:0.05–1.4%) had HPV 18, 21 (4%;95%CI 3–6%) had more than one genotype detected (range 2–7 types), and 21 (4%;95%CI: 3–6%) had at least one of the vaccine-preventable genotypes (6,11,16,18). Of the 251 HIV negative men, 17 (7% 95% CI: 4–11%) had at least one HPV type compared to 48 (19% 95%CI: 15–25%) of the 249 HIV-positive men, p<0.001 (Table 1).
Sensitivity of Sampling Methods
Of the 65 samples positive on any of the three sampling methods, the number positive for each method was: oral rinse 63 (sensitivity 97%, 95%CI: 89–100%), tampon-absorbed oral rinse 45 (sensitivity 69%, 95%CI: 57–80%) and swab 21 (sensitivity 32%, 95%CI: 21–45%) (Table 2). The order in which the samples were collected did not influence the proportion of swabs or rinses that were positive for at least one type of HPV (P = 0.93).
Risk Factor Analysis
Table three shows the crude odds ratios for the three sexual behaviours (tongue-kissing, oral-penile sex, and oral-anal sex) over the three non-overlapping time periods (0–2 weeks,>2 weeks to one year, one year to lifetime) and only the number of sexual partners more than a year ago was significantly associated with HPV detection.
Table three also shows, for each sexual practice, the adjusted odds ratios when all three time periods are included in the model. Only the number of partners more than a year ago was significantly associated with HPV detection, and only for tongue-kissing and oral-penile sex (Table 3).
Table four shows crude odds ratios for factors significantly associated with HPV detection. These were HIV infection, older age, more recent brushing of teeth, current smoking, ever having anogenital warts, and ejaculation occurring more commonly with oral sex (Table 4).
HPV detection was not associated with: the number of days since last oral-penile sex (P = 0.04) or tongue-kissing (P = 0.4), hours since last ate (P = 0.4) or drank (P = 0.5), whether or not condoms were used for half or more oral-penile sex partners (P = 0.9), current or nadir CD4 T cell count (P>0.3 for both) or HIV viral load (P = 0.5) (if HIV infected. Data not shown).
Table four shows the adjusted odds ratios in two logistic regression models. The first model includes lifetime number of tongue-kissing partners, and the second includes lifetime number of oral-penile sex partners. These two practices were strongly correlated (chi-squared p<0.001). In both models, HPV detection was significantly associated with current smoking, HIV infection, more recent brushing of teeth and the lifetime number of sexual partners for either tongue-kissing or oral-penile sex.
Twelve of the 13 men with HPV 16 or 18 were retested after a median of six months (185 days, range 139–211) and 10 of the 12 (83%) remained positive for the same genotype.
Of the 249 HIV positive men, 37 were involved in a subsequent ongoing study of HIV-positive MSM aged ≥35 years (personal communication TRH Read) where anal samples were taken a mean of 16 months after the oral samples. Of these 37 men 35, 95%(95% CI 87%–100%) tested positive for anal HPV. Of the 13 with anal HPV 16, 35%(95% CI 19%–51%), only one had oral HPV 16 detected (Table 5).
In our study of sampling and transport methods, oral rinse samples were significantly more sensitive than self-collected swabs and absorbing the oral rinse into a tampon for postage resulted in a significantly lower rate of oral HPV detection. HPV detection was more likely in those who had recently brushed their teeth and in current smokers. A higher number of lifetime sexual partners for tongue kissing and oral-penile sex, also predicted oral HPV detection. However only a minority of men with high numbers of oral sex partners were HPV positive, and prevalence in the mouth was much lower than prevalence in the anus, which was high in this and other studies of MSM. ,  The same genotype of HPV was detected in 83% of the 12 men retested six months later. Together these data suggest that oral HPV infection may be difficult to acquire, but once present may persist many years.
The finding that recent tooth-brushing increases HPV detection also suggests that current sampling techniques may be improved by prior epithelial abrasion, similar to that used for anogenital HPV detection in men.  The likelihood of detecting oral HPV fell in a linear fashion by about 14% with each additional hour after brushing teeth, suggesting that abrasion of oral mucosa improves collection of infected cells in an oral rinse. D’Souza and coworkers have reported an association between oropharyngeal cancer and infrequent toothbrushing, but this has not been reported for HPV detection and may be related to a different causal pathway for oropharyngeal cancer.  These investigators have combined oropharyngeal brushings with oral rinses ,  and have shown higher detection in oral rinses than in brushings  but there are no reports comparing HPV detection in oral rinse samples with and without prior abrasion. Oral HPV detection was also associated with current smoking and this also has been reported by D’Souza. ,  Smoking causes oral epithelial thickening and periodontal disease  and given our observation that epithelial abrasion increases HPV detection, it may be that the epithelial effects of smoking contribute to increased detection in smokers.
Reports differ on whether oral HPV is significantly associated with recent or lifetime numbers of sexual partners , , . There are three potential reasons for these differences. Firstly, if only young adults within a few years of onset of sexual activity are studied, recent partners may approximate lifetime partners. This explanation is suggested by the observation that oral HPV is significantly associated with recent partners in studies that involve only young adults (age<24  or<27 ). Secondly, most studies are of women and heterosexual men with fewer partners than the MSM population assessed in the present study. Finally, we tested the association of oral HPV with partner numbers in non-overlapping time periods, whereas other investigators have not separated them.
We found that HPV detection rose with age, consistent with most other studies , ,  and importantly no studies have shown significantly declining prevalence with age. An increasing prevalence of HPV with age is consistent with the finding that lifetime, but not recent partner numbers, are most strongly associated with oral HPV detection suggesting that once acquired, oral HPV infection persists a long time. However if this is true the relatively low prevalence of HPV infection, despite a high numerical sexual exposure can only be explained if oral HPV infection is difficult to acquire. These findings contrast with cervical HPV infection which is acquired rapidly after commencing sexual activity, is related to recent sexual partners and its prevalence falls with age. , , 
Our study had a number of limitations. Firstly, it was cross-sectional and the associations are subject to the limitations of this design, such as unmeasured or incomplete adjustment for confounding. Secondly, the participants were from one sexual health service and therefore it may not be reasonable to generalise these findings to populations with a lower sexual risk. Finally, the reported number of sexual partners may have been affected by recall or social desirability bias.
Future studies could examine the effect of brushing prior to obtaining oral rinse samples to enhance HPV detection. We found a high level of HPV persistence in the small number of men tested after six months. Larger longitudinal studies are required to confirm this and to establish the age of acquisition of oral HPV to inform future vaccination policies.
Conceived and designed the experiments: CKF TRHR SNT MJM SMG MYC CSB JSH. Performed the experiments: LAV SNT TRHR MJM. Analyzed the data: TRHR JSH CKF AEG. Contributed reagents/materials/analysis tools: SNT SMG. Wrote the paper: TRHR CKF JSH AEG. Critical revision of manuscript: JSH LAV SNT MJM AEG CSB MYC SMG CKF.
- 1. Gillison ML, Koch WM, Capone RB, Spafford M, Westra WH, et al. (2000) Evidence for a causal association between human papillomavirus and a subset of head and neck cancers. J Natl Cancer Inst 92: 709–720. doi: 10.1093/jnci/92.9.709
- 2. Hong AM, Grulich AE, Jones D, Lee CS, Garland SM, et al. (2010) Squamous cell carcinoma of the oropharynx in Australian males induced by human papillomavirus vaccine targets. Vaccine 28: 3269–3272. doi: 10.1016/j.vaccine.2010.02.098
- 3. Kreimer AR, Clifford GM, Boyle P, Franceschi S (2005) Human papillomavirus types in head and neck squamous cell carcinomas worldwide: a systematic review. Cancer Epidemiol Biomarkers Prev 14: 467–475. doi: 10.1158/1055-9965.epi-04-0551
- 4. Hong A, Grulich A, Jones D, Lee S, Garland S, et al. (2010) Oropharyngeal cancer. Australian data show increase. Bmj 340: c2518. doi: 10.1136/bmj.c2518
- 5. Hocking JS, Stein A, Conway EL, Regan D, Grulich A, et al. (2011) Head and neck cancer in Australia between 1982 and 2005 show increasing incidence of potentially HPV-associated oropharyngeal cancers. Br J Cancer 104: 886–891.
- 6. Sturgis EM, Cinciripini PM (2007) Trends in head and neck cancer incidence in relation to smoking prevalence: an emerging epidemic of human papillomavirus-associated cancers? Cancer 110: 1429–1435. doi: 10.1002/cncr.22963
- 7. D’Souza G, Agrawal Y, Halpern J, Bodison S, Gillison ML (2009) Oral sexual behaviors associated with prevalent oral human papillomavirus infection. J Infect Dis 199: 1263–1269. doi: 10.1086/597755
- 8. D’Souza G, Fakhry C, Sugar EA, Seaberg EC, Weber K, et al. (2007) Six-month natural history of oral versus cervical human papillomavirus infection. Int J Cancer 121: 143–150. doi: 10.1002/ijc.22667
- 9. Gillison ML, Broutian T, Pickard RK, Tong ZY, Xiao W, et al. (2012) Prevalence of oral HPV infection in the United States, 2009–2010. Jama 307: 693–703. doi: 10.1001/jama.2012.101
- 10. Kreimer AR, Alberg AJ, Daniel R, Gravitt PE, Viscidi R, et al. (2004) Oral human papillomavirus infection in adults is associated with sexual behavior and HIV serostatus. J Infect Dis 189: 686–698. doi: 10.1086/381504
- 11. Marais DJ, Passmore JA, Denny L, Sampson C, Allan BR, et al. (2008) Cervical and oral human papillomavirus types in HIV-1 positive and negative women with cervical disease in South Africa. J Med Virol 80: 953–959. doi: 10.1002/jmv.21166
- 12. Steinau M, Reddy D, Sumbry A, Reznik D, Gunthel CJ, et al.. (2012) Oral sampling and human papillomavirus genotyping in HIV-infected patients. J Oral Pathol Med.
- 13. AustraliaPost (2009) Dangerous & Prohibited Goods & Packaging Post Guide.
- 14. Parisi SG, Cruciani M, Scaggiante R, Boldrin C, Andreis S, et al. (2011) Anal and oral human papillomavirus (HPV) infection in HIV-infected subjects in northern Italy: a longitudinal cohort study among men who have sex with men. BMC Infect Dis 11: 150. doi: 10.1186/1471-2334-11-150
- 15. Read TR, Hocking JS, Chen MY, Donovan B, Bradshaw CS, et al. (2011) The near disappearance of genital warts in young women 4 years after commencing a national human papillomavirus (HPV) vaccination programme. Sex Transm Infect 87: 544–547. doi: 10.1136/sextrans-2011-050234
- 16. Gravitt PE, Peyton CL, Alessi TQ, Wheeler CM, Coutlee F, et al. (2000) Improved amplification of genital human papillomaviruses. J Clin Microbiol 38: 357–361.
- 17. Garland SM, Tabrizi SN, Chen S, Byambaa C, Davaajav K (2001) Prevalence of sexually transmitted infections (Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis and human papillomavirus) in female attendees of a sexually transmitted diseases clinic in Ulaanbaatar, Mongolia. Infect Dis Obstet Gynecol 9: 143–146. doi: 10.1155/s1064744901000254
- 18. Stevens MP, Garland SM, Rudland E, Tan J, Quinn MA, et al. (2007) Comparison of the Digene Hybrid Capture 2 assay and Roche AMPLICOR and LINEAR ARRAY human papillomavirus (HPV) tests in detecting high-risk HPV genotypes in specimens from women with previous abnormal Pap smear results. J Clin Microbiol 45: 2130–2137. doi: 10.1128/jcm.02438-06
- 19. Stevens MP, Garland SM, Tabrizi SN (2008) Validation of an automated detection platform for use with the roche linear array human papillomavirus genotyping test. J Clin Microbiol 46: 3813–3816. doi: 10.1128/jcm.01169-08
- 20. Wentzensen N, Schiffman M, Dunn T, Zuna RE, Gold MA, et al. (2009) Multiple human papillomavirus genotype infections in cervical cancer progression in the study to understand cervical cancer early endpoints and determinants. Int J Cancer 125: 2151–2158. doi: 10.1002/ijc.24528
- 21. Vajdic CM, van Leeuwen MT, Jin F, Prestage G, Medley G, et al. (2009) Anal human papillomavirus genotype diversity and co-infection in a community-based sample of homosexual men. Sex Transm Infect 85: 330–335. doi: 10.1136/sti.2008.034744
- 22. Weaver BA, Feng Q, Holmes KK, Kiviat N, Lee SK, et al. (2004) Evaluation of genital sites and sampling techniques for detection of human papillomavirus DNA in men. J Infect Dis 189: 677–685. doi: 10.1086/381395
- 23. D’Souza G, Kreimer AR, Viscidi R, Pawlita M, Fakhry C, et al. (2007) Case-control study of human papillomavirus and oropharyngeal cancer. N Engl J Med 356: 1944–1956. doi: 10.1056/nejmoa065497
- 24. Taybos G (2003) Oral changes associated with tobacco use. Am J Med Sci 326: 179–182. doi: 10.1097/00000441-200310000-00005
- 25. Beachler DC, Weber KM, Margolick JB, Strickler HD, Cranston RD, et al. (2012) Risk factors for oral HPV infection among a high prevalence population of HIV-positive and at-risk HIV-negative adults. Cancer Epidemiol Biomarkers Prev 21: 122–133. doi: 10.1158/1055-9965.epi-11-0734
- 26. Brown B, Blas MM, Cabral A, Carcamo C, Gravitt PE, et al. (2011) Oral sex practices, oral human papillomavirus and correlations between oral and cervical human papillomavirus prevalence among female sex workers in Lima, Peru. Int J STD AIDS 22: 655–658. doi: 10.1258/ijsa.2011.010541
- 27. Ho GY, Bierman R, Beardsley L, Chang CJ, Burk RD (1998) Natural history of cervicovaginal papillomavirus infection in young women. N Engl J Med 338: 423–428. doi: 10.1056/nejm199802123380703
- 28. Winer RL, Lee SK, Hughes JP, Adam DE, Kiviat NB, et al. (2003) Genital human papillomavirus infection: incidence and risk factors in a cohort of female university students. Am J Epidemiol 157: 218–226. doi: 10.1093/aje/kwf180