Unlike the laminar arrangement of neurons in the neocortex, thalamic neurons aggregate to form about dozens of nuclei, many of which make topographic connections with specific areas in the neocortex. The molecular mechanisms underlying the formation of thalamic nuclei remain largely unknown. Homeodomain transcription factor Gbx2 is specifically expressed in the developing thalamus. Deleting Gbx2 leads to severe disruption of the histogenesis of the thalamus in mice, demonstrating an essential role of Gbx2 in this brain structure. Using inducible genetic fate mapping, we have previously shown that the neuronal precursors for different sets of thalamic nuclei have distinctive onset and duration of Gbx2 expression, suggesting that the dynamic expression of Gbx2 plays an important role in the specification and differentiation of thalamic nuclei. Here, we showed that the Gbx2 lineage exclusively gives rise to neurons but not glia in the thalamus. We performed conditional deletion to examine the temporal requirements of Gbx2 in the developing thalamus in mice. Corresponding to the dynamic and differential expression of Gbx2 in various thalamic nucleus groups, deleting Gbx2 at different embryonic stages disrupts formation of distinct sets of thalamic nuclei. Interestingly, different thalamic nuclei have remarkably different requirements of Gbx2 for the survival of thalamic neurons. Furthermore, although Gbx2 expression persists in many thalamic nuclei until adulthood, only the initial expression of Gbx2 following neurogenesis is crucial for the differentiation of thalamic nuclei. Our results indicate that the dynamic expression of Gbx2 may act as an important determinant in coupling with other developmental programs to generate distinct thalamic nuclei.
Citation: Li K, Zhang J, Li JYH (2012) Gbx2 Plays an Essential but Transient Role in the Formation of Thalamic Nuclei. PLoS ONE7(10): e47111. https://doi.org/10.1371/journal.pone.0047111
Editor: Xin Zhang, Indiana University School of Medicine, United States of America
Received: July 27, 2012; Accepted: September 12, 2012; Published: October 4, 2012
Copyright: © Li et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by the National Institute of Health R01 MH094914 to JL. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
The mammalian thalamus is composed of dozens of nuclei formed by aggregates of neurons, and each nucleus displays unique cytoarchitecture and connectivity . The principal nuclei, which project topographically to specific areas of the cortex, have a primary role in processing and relaying periphery sensory input to the cortex, while other nuclei project broadly to the cortex and regulate the state of consciousness , . Virtually all thalamic neurons are born between embryonic day (E) 10.5 and E16.5 in mice . Between E14.5 and E18.5, the thalamus is gradually partitioned into discrete neuronal groups, signifying the differentiation of thalamic nuclei . The individual thalamic nucleus becomes recognizable after birth in mice . Currently, little is known about the molecular and cellular mechanisms that govern the specification, differentiation, and selective aggregation of thalamic neurons to form distinct nuclei.
The expression of Gbx2 (Gastrulation brain homeobox gene 2) is initially detected in postmitotic cells of the thalamus in mouse embryos at E10.5 , , , . Deletion of Gbx2 results in nearly complete loss of thalamocortical projections, and severe defects in the histogenesis of the thalamus , , , . In both mice and monkeys, Gbx2 expression is maintained in a subset of thalamic nuclei to adult stages, suggesting that Gbx2 has an evolutionarily conserved function in the establishment and maintenance of thalamic nuclei . Using a Gbx2creER knock-in mouse line, we have previously performed genetic inducible fate mapping and demonstrated that the Gbx2-lineage contributes to the entire thalamic complex . Significantly, different thalamic nuclei have distinct onset and different duration of Gbx2 expression, suggesting that the dynamic change of Gbx2 expression may control the formation of thalamic nuclei .
Mice deficient for Gbx2 die at birth probably due to defects in the anterior hindbrain . The neonatal lethality and the severe thalamus malformation have precluded the dissection of Gbx2 function in different thalamic nuclei in Gbx2 global knockout mutants. To circumvent these difficulties, we performed conditional knockout (CKO) experiments to remove Gbx2 at different embryonic stages. These Gbx2-CKO mutant mice were viable, thus allowing examination of the formation of thalamic nuclei when they became clearly discernable at postnatal stages. Our study has revealed an essential but transient requirement of Gbx2 in the formation of various thalamic nuclei.
(A–G) Anti-GFP staining of YFP, which is expressed from the R26RYFP locus and marks the descendants of Gbx2-expressing cells labeled at E10.5 (A–D) or E14.5 (F–G), and neuronal or glial markers as indicated on coronal sections of P21 Gbx2creER/+; R26RYFP mice. Asterisks indicate the significantly large number of GAFP+ cells in the IGL. (H–I) Double labeling of BLBP and NeuN or CC1 on sections of P21 thalamus. Nuclei are stained with TOPO3 (blue). Scale bars: A 100 µm; B (for B–I) 20 µm.
Materials and Methods
Mouse and tissue preparation
All animal procedures described herein were approved by the Animal Care Committee at the University of Connecticut Health Center. Mice were housed in a facility with a 12 h light/dark cycle and allowed free access to food and water. All mouse strains were maintained on a CD1 genetic background (Charles River Lab, Wilmington, MA). Adult mice were euthanized by carbon dioxide narcosis and followed by cervical dislocation. Neonatal mice were sacrificed by decapitation. Noon of the day on which a vaginal plug was detected was designated as E0.5 in staging of embryos. The Gbx2creER allele contains creER-ires-EGFP insertion at the 5′UTR so that both creER and EGFP are simultaneously expressed mimicking the endogenous Gbx2 expression . For creER-mediated gene deletion, Gbx2creER/+ males were bred with Gbx2F/+; R26RlacZ/lacZ or Gbx2F/F; R26RlacZ/lacZ females, which carried the Gbx2floxed conditional allele (Gbx2F)  and a cre reporter, R26RlacZ . Four to six milligrams of tamoxifen (Sigma, St. Louis, MO) in corn oil were administered to pregnant females by oral gavage using feeding needles. Embryos with a high percentage of recombination were selected by strong X-gal staining in the hindbrain. The recombination was subsequently confirmed by X-gal histochemistry on thalamus sections. Two additional cre-reporter lines, R26RYFP , and R26RRFP , were used in this study as indicated in the text. Immunofluorescence for GFP detected both EGFP and YFP from Gbx2creER and R26RYFP alleles, respectively, during embryonic stages. However, EGFP expression from the Gbx2creER locus became greatly reduced so that GFP antibody staining mainly detected the robust YFP signals. Embryos and mice carrying the Gbx2creER allele were identified by EGFP fluorescence in the hindbrain and spinal cord. Other alleles were determined by PCR analysis of tail DNA as described previously , .
(A) Diagram of temporal cohorts of Gbx2-expressing cells giving rise to different groups of thalamic nuclei. Gbx2 was deleted following administration of tamoxifen at E10.5, E14.5, or E16.5, as indicated by red arrows and the histology of thalamic nuclei was examined at P4 or P21 (Fig. 3 and 4). (B) Schematic representation of breeding scheme and “self-deletion” of Gbx2 by creER-mediated recombination in Gbx2creER/F embryos. (C–N) ISH for Gbx2 on coronal sections corresponding to the rostral (C–H) and caudal level (I–N) of the thalamus in control and Gbx2-CKO embryos between E14.5 and E18.5. The age of embryos and the stage of tamoxifen administration are indicated to the left. The RNA probe recognizes the Gbx2 sequence that will be deleted by creER-mediated recombination. Note that the Gbx2 transcripts are missing in the presumptive MG (red asterisk) following tamoxifen administration at E10.5, E14.5, or E16.5. By contrast, Gbx2 expression in the rostral-medial thalamus (blue asterisk) is mostly unaffected by deletion at E10.5. Abbreviations of thalamic nuclei are listed in table I. Scale bar in A: 250 µm (for A–L); 237 µm (for G–N).
Embryonic mouse brains were dissected in cold phosphate buffered saline (PBS) and fixed in 4% paraformaldehyde (PFA) for 40 minutes. To analyze postnatal brains, mice were anesthetized by intraperitoneal injection of 2,2-tribromoethanol and followed by transcardiac perfusion with PBS and 4% PFA, and the dissected brains were fixed in 4% PFA for 3 hours. Brains were cryoprotected in 30% sucrose in PBS, frozen in OCT freezing medium (Sakura Finetek), sectioned into 20 μm thickness.
(A–P) CO histochemistry on coronal brain sections of control (A–D) and Gbx2creER/F (E–P) mice at P4 that were given tamoxifen at E10.5 (E–H), E14.5 (I–L) or E16.5 (M–P). Thalamic nuclei are outlined in control sections and the affected thalamic nuclei are listed in blue at the lower left corner. Identification and nomenclature of thalamic nuclei are based on Caviness and Frost  and Jones . The asterisk marks the loss of tissue corresponding to the anterior-medial group of nuclei (I), and the double arrow indicates the reduced medal-lateral dimension of VB (G). Note that thalamic nuclei are indistinguishable between control and Gbx2-CKO mice receiving tamoxifen at E16.5. Scale bar in P: 500 µm (for A–P).
Histochemistry, immunofluorescence, and in situ hybridization
Standard protocols were used for X-gal histochemistry, immunofluorescence, and in situ hybridization (ISH), as described previously . For cytochrome oxidase (CO) staining, sections were stained in freshly prepared solution containing 0.5 mg/ml diaminobenzidine (Sigma), 0.2 mg/ml cytochrome C (Sigma), and 40 mg/ml sucrose in PBS as described previously . The incubation period varied from 14 to 18 hours, and the reaction was terminated based on empirical observations. Detailed protocols for histochemistry, immunostaining and ISH are available in the Li Laboratory website (http://lilab.uchc.edu/Pages/Protocols.html). Primary antibodies used in the study were as follows: rabbit anti-GFP (1∶1000, Invitrogen), rat anti-GFP (1∶2000, Nacalai Tesque), rabbit anti-caspase 3 (cleaved) (1∶500, Cell Signaling Technology), rabbit anti-5-HTT (1∶1000, ImmunoStar), mouse anti-Nkx2.2 (1∶200, DSHB), rabbit anti-NPY (1∶25000, Dr. Richard Mains, University of Connecticut Health Center), rabbit anti-Sox2 (1∶250, R&D Systems), rabbit anti BLBP (1∶1000) and mouse anti-NeuN (1∶200, Millipore), and mouse anti-CC1 (1∶100, Calbiochem). Alexa fluorescent secondary antibodies (Invitrogen) were used.
(A–L) ISH for Tnnt1 on coronal sections of P21control (A–C) and Gbx2creER/F brains that was given tamoxifen at E10.5 (D–F), E14.5 (G–I) and E16.5 (J–L), respectively. Insets in (C) and (F) show ISH for Prkcd. The affected nuclei are listed in the lower left corner or indicated by an asterisk. The double arrow indicates the reduced size of VB; red arrowheads show residual Tnnt1+ and Prkcd+ cells in the presumptive MG nucleus; the white arrowhead indicates LP in G (this section corresponds a slightly more caudal position than those in A, D, and J). Abbreviations: mt, mamillothalamic tract; S, submedial nucleus. Scale bar in L: 500 µm (for A–L).
All phenotypes reported were consistently observed in at least three Gbx2-CKO mutants. To quantify cell death, double immunofluorescence for GFP and activated caspase 3 (Casp3) was performed on serial coronal sections of E16.5 or E18.5 brains. Thalamic sections were matched at five thalamic rostrocaudal levels per genotype. The outline of different pronuclei was identified with the help of EGFP expression from the Gbx2creER locus. The total number of Casp3-positive cells was determined for each pronucleus from the same number of matched sections between control and mutant brains. At least three control or three Gbx2-CKO embryos were analyzed to determine the average number of dead cells. Statistical difference was determined by Student's t-test using Prism (GraphPad Software).
(A–C) Double immunofluorescence for Sox2 and GFP, which reflects the transcription of the Gbx2 locus, on coronal sections of E18.5 brains of indicated genotypes. Boxed areas are magnified in E–G'. The asterisk indicates increased GFP expression in MD, and reduction in size of the VB; the dashed lines mark the border between Sox2 and GFP corresponding to the dLG and LP border; the brackets indicate the width of a band of strong Sox2-expressing cells along the lateral side of dLG and LP. (D) A schematic diagram indicates the position and the plane of sections shown in the figure. (H–M) Immunofluorescence for Nkx2.2 and NPY on coronal sections of E18.5 brains of indicated genotypes. Asterisks indicate the reduction of NPY+ neurites and the smaller VB in I; arrows mark NPY+ cells in the IL. Scale bars: A (A–C) 200 µm; E (for E–G'') 50 µm.
Gbx2-expressing cells exclusively give rise to thalamic neurons
We have previously shown that Gbx2 is mainly expressed in postmitotic cells in the thalamus . To determine the neural lineage derived from Gbx2-expressing cells, we performed genetic inducible fate mapping in Gbx2creER/+; R26RYFP/+ mice to permanently label Gbx2-expressing cells in between E10.5 and E16.5. The identity of the fate-mapped cells was examined by markers for neurons (NeuN), oligodendrocytes (CC1 and BLBP), and astrocytes (GFAP) at postnatal stages. At postnatal day (P) 21, all descendants of Gbx2-expressing cells that were labeled at E10.5, E14.5 or E16.5 were positive for NeuN, but negative for CC1 or GFAP (Fig. 1A–F and data not shown). There were many BLBP+ cells in the thalamus at P21 (Fig. 1G–I). These BLBP+ cells were negative for NeuN, CC1, or GFAP, suggesting that these BLBP+ cells are probably glial precursor cells (Fig. 1H, I, and data not shown). Importantly, none of the Gbx2-derived cells were positive for BLBP (Fig. 1G and data not shown). Therefore, the Gbx2 lineage exclusively gives rise to neurons but not glia in the thalamus.
(A–D) Immunofluorescence of activated Casp3 on coronal sections of the thalamus of indicated genotypes. GFP signals in A and B were derived from the Gbx2cereER allele. MG (A and B) and the dorsomedial thalamus (C and D) are outlined by the dashed lines. Arrows indicate apoptotic cells. (E) Histogram representation of the average number of apoptotic cells in different thalamic nuclei. Error bars indicate standard deviations. Asterisks * and ** indicate p< 0.05 and p<0.001, respectively (Student's t-test). (F–I) X-gal histochemistry on coronal brain sections of P4 mice that were given tamoxifen at E10.5. The double arrow indicates reduced size of VB in mutants. Scale bars: B (for A–B) 100 µm; D (for C–D) 200 µm; I (F–I) 250 µm.
Removing Gbx2 at different embryonic stages disrupts distinct sets of thalamic nuclei
By fate-mapping Gbx2-expressing cells between E9.5 and E15.5, we have previously identified five groups of thalamic nuclei (Fig. 2A) . The postmitotic neurons of principal relay nuclei, such as the dLG, VB, LP and MG nuclei, express Gbx2 by E10.5 (Fig. 1A and Table I for abbreviations). The expression of Gbx2 is quickly downregulated in dLG and VB (designated as group I nuclei), yet persists in LP and MG (group II). The second wave of Gbx2-expressing cells (E10.5–E13.5) gives rise to many association nuclei, such as the AV, CL, MD, PCN, CE, and relay nuclei, LD, VL, and VM (Fig. 1A). Among these nuclei, Gbx2 expression persists in MD and CE, PCN and CL (group III) in postnatal stages, but is downregulated in LD and VM (group IV) after E14.5. The last wave of Gbx2-expressing cells (E15.5) gives rise to many anterior-medial nuclei, such as PVT, PT, RE, and AM (Fig. 1A), and Gbx2 expression in these nuclei persists in postnatal stages .
(A–B) Immunohistochemistry for 5-HTT on tangential sections of P7 brains of control and Gbx2-CKO mice that received tamoxifen at E10.5. The 5-HTT immunohistochemistry reveals the body map in the cortex: a, auditory; v, visual; fl, florelimeb; hl, hindlimb; ll, lower lip; t, trunk; lw, large whiskers; sw, small whiskers . (C–F) Red fluorescence (tdTomato) on coronal sections of P21 brains of Gbx2creER/+; R26RRFP/+ (C and E) and Gbx2creER/F; R26RRFP/+ (D and F) mice that were given tamoxifen at E10.5. The primary auditory area is indicated by arrowheads and cortical layers IV and VI were marked by white and green arrows, respectively. Note that the thickness (bracket) of the auditory cortex is reduced in Gbx2-CKO mutants. (E'–F') Nissl histology of the auditory cortex of control (E') and Gbx2-CKO mice (F'). (G–I) ISH for Lmo4 on sagittal sections of E18.5 control and Gbx2-CKO mutants that received tamoxifen at E10.5 or E14.5 as indicated. Black arrowheads demarcate the borders of the somatosensory area. Abbreviations: FC frontal; Occ, occipital; Par, parietal. Scale bar in A: 250 µm (A–B); 154 µm (for C–D); 307 µm (for E–F); 189 µm (for G–I).
To investigate whether the different onset and duration of Gbx2 expression controlled the formation of thalamic nuclei, we deleted Gbx2 at different stages using a “self-deletion” approach in compound heterozygous Gbx2creER/F embryos by activating creER through administering tamoxifen at E9.5, E10.5, E12.5, E14.5, and E16.5 (Fig. 2A) , . The extent of recombination and the resulting phenotype were variable when tamoxifen was administered at E9.5 and E12.5 probably because Gbx2 expression is about initiated at E9.5 and undergoes a major transition from E10.5 to E14.5 (data not shown). Therefore, we mainly characterized the phenotype of Gbx2-CKO mice that were given tamoxifen at E10.5, E14.5, or E16.5 in the current study. To examine the specific deletion of Gbx2, we performed ISH using a Gbx2 probe corresponding to Gbx2 exon II that was deleted by creER-mediated recombination. When tamoxifen was given at E10.5, E14.5, or E16.5, Gbx2 transcripts were mostly absent in the most caudal and lateral region of the thalamus, presumably the MG in Gbx2creER/F embryos (Fig. 2C–H). However, Gbx2 mRNA was present in the anterior and medial region of the thalamus in Gbx2creER/F embryos that received tamoxifen at E10.5. By contrast, Gbx2 transcripts were mostly abolished or reduced in the anterior-medial part of the thalamus, as well as the MG, in Gbx2creER/F embryos that received tamoxifen at E14.5 or E16.5 (Fig. 2I–N). Therefore, the self-deletion approach results in efficient and selective deletion of Gbx2 in different regions of the developing thalamus.
Gbx2creER/F mice that received tamoxifen at E10.5 or later stages were viable and grossly normal. To examine the formation of thalamic nuclei, we performed CO histochemistry of Gbx2-CKO brain at P4 when thalamic nuclei were readily discernable (Fig. 3A–D). When Gbx2 was deleted around E10.5, MG was mostly missing, and VB appeared smaller (Fig. 3G and H). The borders among group III nuclei (e.g. MD, Cl, and Ce) became less prominent, suggesting abnormal differentiation and/or aggregation of these nuclei (Fig. 3E and F). Although administration of tamoxifen at E14.5 resulted in efficient removal of Gbx2 transcripts in the caudal and lateral thalamus (Fig. 2I), the MG and VB nuclei were mostly indistinguishable between control and the Gbx2-CKO mice (Fig. 3J–L). By contrast, deleting Gbx2 at E14.5 disrupted group III nuclei, such as MD, Cl, and Ce (Fig. 3J). Furthermore, tissue corresponding to many group V nuclei (e.g. PT, PVT, and RE) was mostly missing following inactivation of Gbx2 at E14.5 (Fig. 3I). In contrast to malformation of various thalamic nuclei due to Gbx2 deletion at earlier stages, the CO histochemistry of the thalamus in Gbx2-CKO mice that received tamoxifen at E16.5 was indistinguishable from that of control mice (Fig. 3I–L).
To further characterize the thalamic nuclei affected by the loss of Gbx2, we examined the transcripts of Tnnt1 (Troponin T type 1) and Prkcd (Protein kinase C delta), which displayed distinct levels of expression in different thalamic nuclei and thus demarcated a subset of thalamic nuclei at P21 (Fig. 4A–C). In agreement with the CO histochemistry, deletion of Gbx2 at E10.5, but not at E14.5 or E16.5, resulted in mostly absence of the MG nucleus with only a few clusters of remnant Tnnt1+ or Prkcd+ cells in the presumptive MG area (Fig. 4E, F, H, and I). Furthermore, the VB, VL, and AV nuclei were noticeably smaller in Gbx2creER/F mice that received tamoxifen at E10.5 (Fig. 4E). In control mice, Tnnt1 was expressed in MD (Fig. 4A and B). When Gbx2 was deleted at E14.5, but not E10.5 nor E16.5, Tnnt1+ cells in the presumptive MD were mostly missing, in support of the notion that group III nuclei were particularly sensitive to the deletion of Gbx2 at E14.5 (Fig. 4G and H). No changes in the expression of Tnnt1 and Pcp4 were found in the thalamus of Gbx2creER/F mice given tamoxifen at E16.5 (Fig. 4J–L and data not shown), demonstrating that Gbx2 is no longer required for thalamic histogenesis by E16.5.
In summary, deletion of Gbx2 at different stages disrupts the formation of distinct thalamic nuclei. Interestingly, the MG formation is unaffected when Gbx2 is deleted after E14.5 despite the persistent Gbx2 expression in this nucleus. Furthermore, deleting Gbx2 at E16.5 has no noticeable effect on the thalamus even though many group III and V nuclei that depend on Gbx2 function at E14.5 continue expressing Gbx2 after birth. These findings demonstrate that Gbx2 is mainly required in a narrow time window immediately after the onset of its transcription for the development of thalamic nuclei.
The initial expression of Gbx2 is essential for all principal thalamic nuclei
We have previously shown that all principal thalamic nuclei are generated from thalamic postmitotic neurons that express Gbx2 between E9.5 and E10.5 . Unexpectedly, no obvious defect in the dLG and LP nuclei was detected by CO histochemistry and Tnnt1/Prkcd expression analyses (Fig. 3 and 4). We thus used additional molecular markers to examine the formation of these nuclei. Sox2 and Gbx2 are differentially expressed in different sets of thalamic nuclei . We performed double immunofluorescence for Sox2 and GFP. The latter was expressed from the Gbx2creER allele and thus mimicked the endogenous Gbx2 expression . Robust GFP expression was detected in LP, CL and PV, and a lower level of GFP expression was observed in MD nucleus in control Gbx2creER/+ embryos that received tamoxifen at E10.5 (Fig. 5A). GFP was mostly absent from dLG while its expression was maintained in LP (Fig. 5A and E'). Complementary to GFP expression, Sox2 was present in VB, dLG, and PO but absent from LP, except for a strip of strong Sox2-expressing cells along the lateral side of dLG and LP (Fig. 5A and E). In Gbx2creER/F embryos that received tamoxifen at E10.5, the conspicuous dLG/LP border defined by the common Sox2/GFP border was lost with many GFP+ cells present in dLG (Fig. 4B, F and F'). The low level of Sox2 expression in dLG was mostly missing, whereas the strip of strong Sox2+ domain along the lateral side of dLG and LP became much wider (Fig. 5B and F). In agreement with CO and marker analyses described above, deleting Gbx2 at E14.5 had little effect on the expression of Sox2 and GFP in dLG and LP nuclei (Fig. 5G and G'). Nevertheless, in these embryos, abnormal GFP expression was found in the presumptive MD, suggesting a specific requirement of Gbx2 for the development of the MD nucleus at E14.5 (Fig. 5C, G and G'). These data demonstrate that deletion of Gbx2 at E10.5, but not E14.5, disrupts the differentiation of dLG and LP.
It was recently shown that a narrow band of cells between the zona limitans intrathalamica (ZLI) and the thalamus gives rise to the ventral lateral geniculate body (vLG) and intergeniculate leaflet (IL) nuclei . Enhancing Fgf8 or Shh signaling near the ZLI results in enlarged vLG and IL and a shrunken VB , , a phenotype similar to that found in Gbx2creER/F embryos that received tamoxifen at E10.5. Therefore, we examined the formation of IL, vLG and reticular (RT) nucleus, which are juxtaposed with dLG and VB, using double immunofluorescence for neural peptide Y (NPY) and Nkx2.2 . NPY was expressed in IL and RT, and Nkx2.2 was expressed in vLG (Fig. 5H and K). The expression pattern of NPY and Nkx2.2 was unchanged when Gbx2 was deleted at E10.5 or E14.5, demonstrating that loss of Gbx2 does not affect the formation of the IL, RT and vLG nuclei (Fig. 5I, J, L, and M). Interestingly, NPY+ neurites, which are presumably derived from RT neurons, were greatly reduced in VB of Gbx2creER/F mice that were given tamoxifen at E10.5 (Fig. 5H–M), suggesting that Gbx2 expression is crucial for the differentiation and the innervation by NPY neurites in the VB nucleus.
Altogether, our results demonstrate that the expression of Gbx2 around E10.5 is important for the differentiation of all principal thalamic nuclei in agreement with the predominant contribution of the initial Gbx2 expressing cells to these nuclei.
Gbx2 is differentially required for the survival of neurons in distinct thalamic nuclei
It has been previously shown increased apoptosis in the thalamus in Gbx2-null mutants at E18.5, demonstrating that Gbx2 is required for thalamic neuron survival . However, the identity of thalamic nuclei that are dependent on Gbx2 for cell survival has not been defined. To this end, we examined the expression of activated caspase 3 (Casp3), which marks cells undergoing apoptosis, in the thalamus following inactivation of Gbx2 at different embryonic stages. No noticeable change was found in the number of Casp3+ cells in the thalamus of Gbx2creER/F embryos in 48 h after tamoxifen exposure at E10.5 or E14.5 (data not shown). An increase of Casp3+ cells was first detected in the lateral-caudal region of the thalamus in Gbx2creER/F embryos at E14.5 following tamoxifen administration at E10.5 (data not shown). As it is difficult to identify the affected nuclei at E14.5, we focused our analysis on E16.5 when some thalamic pronuclear masses begun to be discernable. Significantly increased Casp3+ cells were restricted to the presumptive LP and MG nuclei following deletion of Gbx2 at E10.5 (Fig. 6A, B, and E). By contrast, Casp3 expression in the presumptive dLG and VB region was indistinguishable between controls and mutants (Fig. 6E). When Gbx2creER/F embryos were given tamoxifen at E14.5, significant increase in the number of Casp3+ cells was found in RE, CL, PO, MD, and nuclei in the anterior-medial groups (AMG) at E18.5 (Fig. 6C, D and E).
To confirm that cell death accounts for the loss of MG and LP, we followed the fate of cells that undergone creER-mediated recombination in Gbx2creER/F mice carrying the cre reporter R26RlacZ. Although X-gal expression is not necessarily indicative for Gbx2-deficiency at the cellar level because the Gbx2-floxed allele and the reporter allele may have different recombination efficiency, the recombination at these two loci should be in agreement with each other in a particular cell cohort. In P4 Gbx2creER/F; R26R+/lacZ mice that were given tamoxifen at E10.5, X-gal histochemistry revealed that the density of ß-gal+ cells was noticeably reduced in the LP and MG, but was unchanged in the dLG and VB nuclei (Fig. 6F–I). Although not all marked cells were necessary mutant, the specific loss of X-gal positive cells in the MG, together with the result of Casp3 analyses, show that Gbx2 is essential for neuron survival in MG and LP, but not in dLG and VB nuclei.
In summary, although loss of Gbx2 disrupts formation of the majority of thalamic nuclei, only a subset of thalamic nuclei, such as the MG, L, RE, CL, and PO, depends on Gbx2 for cell survival.
Parcellation of the cortex is mostly intact in Gbx2-CKO mutants
The principal thalamic nuclei project to specific sensory cortical areas. We next examined the innervation of thalamocortical axons (TCAs) in the sensory cortical regions. TCAs innervating the cerebral cortex in neonatal mice transiently store serotonin (5-HT) in synaptic vesicles by expressing serotonin transporter (5-HTT) . Therefore, immunohistochemistry for 5-HT or 5-HTT reveals the body map in layer IV of the cortex in P7 mice  (Fig. 7A and data not shown). In agreement with the loss of the MG nuclei, which innervate the auditory cortex (A1), 5-HTT immunoreactivity in the A1 area was greatly reduced in Gbx2-CKO mutants that were given tamoxifen at E10.5 (Fig. 7B). Interestingly, although the VB was affected by Gbx2 deletion at E10.5 (Fig. 3G, 4E, 5B and I), the somatosensory barrels were present in Gbx2-CKO mutants (Fig. 7B). Examination by CO histochemistry also showed normal somatosensory barrels in Gbx2-CKO mutants that received tamoxifen at E10.5 (data not shown). To further examine the barrels in the cortex, we used the cre report R26RRFP that expressed robust tdTomato from the ROSA locus , and permanently labeled TCAs following Gbx2-creER mediated recombination. As expected, TCAs were mostly detected in cortical layers IV and VI in Gbx2creER/+; R26R+/RFP and Gbx2creER/F; R26R+/RFP mice that received tamoxifen at E10.5 (Fig. 7C–F). In the somatosensory cortex, RFP+ neurites clearly demarcated the individual barrels in Gbx2-CKO mutants as well as in the control (Fig. 7C and D). In agreement with the specific cell death in MG nucleus, RFP+ neurons were significantly reduced in MG nuclei and RFP+ neurites were noticeable reduced in layers IV and VI in the auditory cortex in Gbx2-CKO mice (Fig. 7E and F). Despite the decrease in the overal thickness, the layered structure of the typical auditory cortex was maintained in Gbx2-CKO mice (Fig. 7E'–F'). To examine the parcellation of the cerebral cortex, we examined the expression of Lmo4 and Lmo3, which demarcated the key subdivision in the cerebral cortex , on a whole series of coronal and sagittal sections of E18.5 brains (Fig. 7G–I and data not shown). No noticeable changes were detected in the expression of these genes in the cortex in Gbx2-CKO embryos at E18.5 when Gbx2 was removed at E10.5 or E14.5.
In summary, we found that deletion of Gbx2 at E10.5 results in greatly reduced TCA projections from the MG and alterations in the auditory cortex. However, the parcellation and the subsequent formation of other body maps, including the sensory barrels, are mostly unaffected by the loss of Gbx2 in the thalamus.
Expression and fate mapping studies suggested that Gbx2 might play an important role in the specification and differentiation of thalamic nuclei , . Indeed, thalamic nuclei, particularly those in the medial, central, and dorsal groups, are severely disrupted in Gbx2 global KO embryos at E18.5 , . However, the severe defects in thalamic histogenesis and neonatal lethality have precluded the analysis of Gbx2 function in any specific thalamic nucleus. Here, we have examined the temporal requirements of Gbx2 and have identified an essential but transient Gbx2 function in the differentiation and/or survival of neurons in different thalamic nuclei.
As arrays of glial cells were previously seen clustered at the boundary of different groups of neurons, it has been proposed that glia may contribute to nuclear formation in the brain . Indeed, we found that the anterior limit of the thalamus was clearly demarcated by a large amount of GFAP+ cells in the IGL and zona limitans intrathalamica (Fig. 1D and data not shown). Compared to the neighboring structures, we found that the matured thalamus contained only a low number of GFAP+ astrocytes (Fig. 1 and data not shown). By contrast, there were a large number of BLBP+ cells in the thalamus (Fig. 1). These BLBP+ cells were negative for NeuN, CC1, and GFAP, and their precise identity remains to be determined. However, we did not detect any regional differences in the distribution of CC1+, GFAP+ or BLBP+ cells within the thalamus. Furthermore, we showed that all the descendants of Gbx2 expressing cells that were labeled at E10.5, E14.5 or E16.5 were positive for NeuN but not CC1, GFAP, or BLBP (Fig. 1 and data not shown), demonstrating an exclusive contribution of the Gbx2 lineage to thalamic neurons. Therefore, it is likely that Gbx2 mainly acts on neurons but not glia during the differentiation of thalamic nucleus.
We have previously shown that the onset of Gbx2 is generally associated with the commencement of neurogenesis of thalamic neurons . Interestingly, although the entire thalamic nuclear complex is derived from the Gbx2-lineage, different groups of nuclei display distinctive temporal expression patterns of Gbx2 . Therefore, the dynamic expression of Gbx2 may act as a determinant for nucleus-specific neurons. Alternatively, Gbx2 is important for assigning a general thalamic identity, which is essential for the subsequent differentiation of the thalamic nucleus. In the current study, we showed that inactivation of Gbx2 disrupted formation of the majority of thalamic nuclei, confirming an essential role of Gbx2 in the formation of thalamic nuclei. Importantly, we demonstrated that deleting Gbx2 at E10.5 or E14.5 mostly affected the same set of nuclei that were composed of fate-mapped Gbx2-expressing neurons labeled at that particular stage (Table I). Furthermore, the nuclei that were specifically affected by Gbx2 deletion at E10.5 or E14.5 were mostly unaffected when Gbx2 was removed at E14.5 or E16.5 regardless of whether Gbx2 expression persists in these nuclei normally. These results demonstrate that Gbx2 plays a crucial role in the formation of various thalamic nuclei in a narrow time window after the onset of its expression. Although our molecular marker analyses were still limited due to the lack of nucleus-specific markers, we have not observed any overt change in nucleus identity in Gbx2-CKO mice (Fig. 4 and 5). In these mutants, the loss or reduction of particular nucleus was not associated with expansion of any neighboring nuclei. Furthermore, by combining fate mapping and Gbx2 deletion, we showed that there were no noticeable changes in the distribution of recombined cells between Gbx2creER/+; R26RlacZ/+ and Gbx2creER/F; R26RlacZ/+ mice (Fig. 6F–I and data not shown). Therefore, we favor the second model in which Gbx2 may be important for newly generated neurons to acquire a general thalamic identity immediately following neurogenesis.
We have extended the previous finding  by defining the thalamic nuclei that depend on Gbx2 for neuron survival. Interestingly, although Gbx2 was dispensable after its initial expression, inactivation of Gbx2 resulted in cell death in groups II, III, and V nuclei that maintain Gbx2 expression but not in groups I and IV nuclei that transiently express Gbx2 (Table I). This bias might result from less efficient deletion of Gbx2 in the latter groups due to transient creER expression. However, this explanation seems unlikely, since we found that the creER-mediated recombination was comparable among nuclei with either transient or prolonged Gbx2 expression by using different cre-reporter lines (Fig. 6F and data not shown). The molecular mechanism by which Gbx2 regulates cell survival in the thalamus is currently unknown. It has been previously shown that the survival of thalamic neurons is partially controlled by trophic factors from the cerebral cortex . In Gbx2 global KO mutants, thalamocortical projections are mostly absent , . Conditional deletion of Gbx2 also resulted in partial loss of thalamocortical axons (Li and Li, unpublished data). Therefore, further studies will determine whether the requirement of Gbx2 for cell survival in a particular nucleus is related to its dependence on Gbx2 to establish axonal connection with the cortex.
Despite the absence of abnormal apoptosis, some nuclei, such as VB, VL, and AV, were decreased in size following Gbx2 deletion at E10.5 (Fig. 3, 4 and 6). This suggests that, in additional to increased cell death, other defects, such as abnormal neurogenesis or altered neuronal migration, may also contribute to the abnormal development of thalamic nuclei in Gbx2-CKO mutants. Although defects were detected in most thalamic nuclei by CO histology and marker analyses, some nuclei appeared unaffected (e.g. AD) or only displayed only subtle defects by Gbx2 deletion at E10.5 or E14.5 (Table I). We cannot rule out that defects may be present in these seeming normal nuclei following Gbx2 deletion at E10.5, E14.5, or even E16.5.
The temporal order of neurogenesis plays an important role in not only the generation of cell type diversity, but also connection diversity of projecting neurons , . Interestingly, in both rodents and primates, the differentiation of thalamic neurons and their connectivity closely relate to the temporal birth order: the early-born neurons tend to form the lateral-posterior nuclei and project to the occipital cortex, while late-born neurons tend to contribute to the medial-anterior nuclei and project to the frontal cortex . We have previously shown that the onset of Gbx2 expression is closely associated with the cell cycle exit of thalamic neuron precursors . In the current study, we found that the initial expression of Gbx2 plays a crucial role in the development of thalamic nuclei. Therefore, the dynamic expression of Gbx2 may act as an important determinant in coupling with other developmental program to generate nucleus-specific neurons. Further studies of the mechanisms underlying the function of dynamic Gbx2 expression would provide important insights into the specification and differentiation of different thalamic nuclei, and subsequently the establishment of topography of TCAs.
We are grateful to Dr. Li Chen for initiating the project, Ms. Qiuxia Guo for excellent technical assistance, Dr. Shen-Ju Chou for providing advice on the examination of cortical sensory map. We thank Drs. Rashmi Bensal, Xue-Jun Li, Richard Mains, for providing the antibodies against CC1, Sox2, and NPY. The Nkx2.2 monoclonal antibody developed by T. Jessell was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by The University of Iowa, Department of Biology, Iowa City, IA 52242.
Conceived and designed the experiments: KL JL. Performed the experiments: KL JZ. Analyzed the data: KL JZ JL. Contributed reagents/materials/analysis tools: KL JZ. Wrote the paper: JL.
- 1. Jones EG (2007) The thalamus. Cambridge; New York: Cambridge University Press.
- 2. Jones EG (2001) The thalamic matrix and thalamocortical synchrony. Trends Neurosci 24: 595–601.
- 3. Angevine JB Jr (1970) Time of neuron origin in the diencephalon of the mouse. An autoradiographic study. J Comp Neurol 139: 129–187.
- 4. Bulfone A, Puelles L, Porteus MH, Frohman MA, Martin GR, et al. (1993) Spatially restricted expression of Dlx-1, Dlx-2 (Tes-1), Gbx-2, and Wnt- 3 in the embryonic day 12.5 mouse forebrain defines potential transverse and longitudinal segmental boundaries. J Neurosci 13: 3155–3172.
- 5. Miyashita-Lin EM, Hevner R, Wassarman KM, Martinez S, Rubenstein JL (1999) Early neocortical regionalization in the absence of thalamic innervation. Science 285: 906–909.
- 6. Chen L, Guo Q, Li JY (2009) Transcription factor Gbx2 acts cell-nonautonomously to regulate the formation of lineage-restriction boundaries of the thalamus. Development 136: 1317–1326.
- 7. Nakagawa Y, O'Leary DD (2001) Combinatorial expression patterns of LIM-homeodomain and other regulatory genes parcellate developing thalamus. J Neurosci 21: 2711–2725.
- 8. Hevner RF, Miyashita-Lin E, Rubenstein JL (2002) Cortical and thalamic axon pathfinding defects in Tbr1, Gbx2, and Pax6 mutant mice: evidence that cortical and thalamic axons interact and guide each other. J Comp Neurol 447: 8–17.
- 9. Szabo NE, Zhao T, Zhou X, Alvarez-Bolado G (2009) The role of Sonic hedgehog of neural origin in thalamic differentiation in the mouse. J Neurosci 29: 2453–2466.
- 10. Jones EG, Rubenstein JL (2004) Expression of regulatory genes during differentiation of thalamic nuclei in mouse and monkey. J Comp Neurol 477: 55–80.
- 11. Wassarman KM, Lewandoski M, Campbell K, Joyner AL, Rubenstein JL, et al. (1997) Specification of the anterior hindbrain and establishment of a normal mid/hindbrain organizer is dependent on Gbx2 gene function. Development 124: 2923–2934.
- 12. Li JY, Lao Z, Joyner AL (2002) Changing requirements for Gbx2 in development of the cerebellum and maintenance of the mid/hindbrain organizer. Neuron 36: 31–43.
- 13. Soriano P (1999) Generalized lacZ expression with the ROSA26 Cre reporter strain [letter]. Nat Genet 21: 70–71.
- 14. Srinivas S, Watanabe T, Lin CS, William CM, Tanabe Y, et al. (2001) Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. BMC Dev Biol 1: 4.
- 15. Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, et al. (2009) A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci 13: 133–140.
- 16. Chen L, Chatterjjee M, Li JY (2010) The mouse homeobox gene Gbx2 is required for the development of cholinergic interneurons in the striatum. J Neurosci.
- 17. Vue TY, Bluske K, Alishahi A, Yang LL, Koyano-Nakagawa N, et al. (2009) Sonic hedgehog signaling controls thalamic progenitor identity and nuclei specification in mice. J Neurosci 29: 4484–4497.
- 18. Vue TY, Aaker J, Taniguchi A, Kazemzadeh C, Skidmore JM, et al. (2007) Characterization of progenitor domains in the developing mouse thalamus. J Comp Neurol 505: 73–91.
- 19. Kataoka A, Shimogori T (2008) Fgf8 controls regional identity in the developing thalamus. Development 135: 2873–2881.
- 20. Persico AM, Mengual E, Moessner R, Hall FS, Revay RS, et al. (2001) Barrel pattern formation requires serotonin uptake by thalamocortical afferents, and not vesicular monoamine release. The Journal of neuroscience: the official journal of the Society for Neuroscience 21: 6862–6873.
- 21. Maier DL, Mani S, Donovan SL, Soppet D, Tessarollo L, et al. (1999) Disrupted cortical map and absence of cortical barrels in growth-associated protein (GAP)-43 knockout mice. Proc Natl Acad Sci U S A 96: 9397–9402.
- 22. Cholfin JA, Rubenstein JL (2007) Patterning of frontal cortex subdivisions by Fgf17. Proceedings of the National Academy of Sciences of the United States of America 104: 7652–7657.
- 23. Steindler DA (1993) Glial boundaries in the developing nervous system. Annu Rev Neurosci 16: 445–470.
- 24. Lotto RB, Asavaritikrai P, Vali L, Price DJ (2001) Target-derived neurotrophic factors regulate the death of developing forebrain neurons after a change in their trophic requirements. The Journal of neuroscience: the official journal of the Society for Neuroscience 21: 3904–3910.
- 25. Petrovic M, Hummel T (2008) Temporal identity in axonal target layer recognition. Nature 456: 800–803.
- 26. McConnell SK, Kaznowski CE (1991) Cell cycle dependence of laminar determination in developing neocortex. Science 254: 282–285.
- 27. Hohl-Abrahao JC, Creutzfeldt OD (1991) Topographical mapping of the thalamocortical projections in rodents and comparison with that in primates. Exp Brain Res 87: 283–294.
- 28. Caviness VS Jr, Frost DO (1980) Tangential organization of thalamic projections to the neocortex in the mouse. J Comp Neurol 194: 335–367.