Dioecy represents a source of variation in plant quality to herbivores due to sexual differences in intensity and timing of resource allocation to growth, defense and reproduction. Male plants have higher growth rates and should be more susceptible to herbivores than females, due to a lower investment in defense and reproduction.
We compared resource investment to growth and reproduction and its consequences to herbivore attack on three Baccharis species along one year (B. dracunculifolia, B. ramosissima, and B. concinna). Phenological patterns presented by the three species of Baccharis were quite different over time, but the number of fourth-level shoots and plant growth rate did not differ between sexes in any studied species. Intersexual difference in reproductive investment was only observed for B. concinna, with female individuals supporting higher inflorescence density than male individuals throughout the year. Gall abundance on the three Baccharis species was not influenced by plant sex. However, all plant traits evaluated here positively influenced the gall abundance on B. concinna, whereas only the number of fourth-level shoots positively influenced gall abundance on B. ramosissima and B. dracunculifolia.
The absence of differential reproductive allocation may have contributed to similar growth and shoot production between the sexes, with bottom-up effects resulting in gender similarities in gall abundance patterns. The number of fourth-level shoots, an indicator of meristem availability to herbivores, was the most important driver of the abundance of the galling insects regardless of host plant gender or species. Albeit the absence of intersexual variation in insect gall abundance is uncommon in the literature, the detailed study of the exceptions may bring more light to understand the mechanisms and processes behind such trend.
Citation: Espírito-Santo MM, Neves FS, Fernandes GW, Silva JO (2012) Plant Phenology and Absence of Sex-Biased Gall Attack on Three Species of Baccharis. PLoS ONE 7(10): e46896. https://doi.org/10.1371/journal.pone.0046896
Editor: Minna-Maarit Kytöviita, Jyväskylä University, Finland
Received: March 13, 2012; Accepted: September 10, 2012; Published: October 4, 2012
Copyright: © Espírito-Santo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This study was supported by Fundação de Amparo à Pesquisa de Minas Gerais (Fapemig) and the Postgraduate Program in Ecologia, Conservação e Manejo da Vida Silvestre of the Universidade Federal de Minas Gerais (UFMG). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Plants have limited resources to invest in growth, reproduction and defense against herbivores . In general, resource demand for these three processes cannot be held simultaneously, due to a tradeoff in resource allocation among these different physiological processes –. Differential resource allocation produces changes in plant traits (e.g. architecture, growth, concentration of carbon-based defensive compounds), that can extend its effects on upper trophic levels , , . Fast-growing species or sexes (within dioecious species; e.g., male plants) are expected to invest less in chemical and/or structural defenses . On the other hand, their counterparts usually invest a higher amount of nutrients in reproduction, and the consequent decrease in nitrogen levels limits the production of new leaves. Thus, female plants or slow-growing species are expected to protect their limited growth potential against tissue loss via herbivory , , .
Plant sex was explored as a source of variation in quality to herbivores in many studies with dioecious species. In general, due to intersexual differences in resource allocation, male plants are more susceptible to herbivore attack than females (the sex-biased herbivory hypothesis) –. Several studies conducted mainly in temperate regions have detected higher herbivory levels on male plants. However, a male-biased herbivore attack has not been corroborated in some recent studies in tropical systems involving Baccharis shrubs , , . Baccharis (Asteraceae) is a genus with over 500 species distributed from United States to Argentina. All species are perennial and dioecious shrubs, except for Baccharis monoica . Baccharis likely supports the richest galling-inducing insects fauna studied so far in the Neotropics, with 121 galls species on 40 host plant species . With such a rich community in which the gall-inducing insects belong to several different taxa, the system comprised of Baccharis species and their gall-inducing insects provides an ideal scenario to test for plant sexual differences on intensity and timing of resource allocation and herbivory attack.
Gall-inducing insects are highly specialized to their host and each species attacks only a single plant organ –. Galling insects are able to manipulate plant chemical defenses , , but are strongly influenced by the production of new tissues (meristems) and/or faster growth or vigorous modules of their hosts –. Thus, attack by these insects can be synchronized to periods in which there is an increased production of highly nutritious young tissues or fast plant growth –. Due to their finely-tuned interaction with the host plants, galling insects provide excellent opportunities to evaluate the influence of bottom-up forces on herbivores , . In a recent meta-analysis on gender effects on plant-herbivore interactions, Cornelissen & Stiling  highlighted the prevalence of galling insects as subjects of sex-biased herbivory studies, representing almost 35% of the independent comparisons reviewed. Moreover, these authors found that the abundances of folivores and galling insects were more strongly influenced by plant sex than other feeding guilds, and intersexual differences in plant morphological, phenological and/or nutritional characteristics might be responsible for this result.
Plant vegetative and reproductive phenology can be influenced by several abiotic (water availability, photoperiod and wind speed, among others) and biotic (presence of herbivores, pollinators and seed dispersers) factors –. In dioecious species, phenology is related to patterns of vegetative and sexual development –, –. Furthermore, trade-offs in biomass allocation between reproduction and growth can lead to temporal variations in vegetative investment in both plant sexes, affecting resource availability for their associated herbivores , , . Male plants usually present a higher investment in flower production early in the reproductive season, whereas females divert most of their energy to sustain fruit maturation later in the reproductive season , . If a trade-off between growth and reproduction occurs, it is expected that males would suffer higher galling attack later in the reproductive season, the contrary being true for female plants. In this way, studies regarding plant intersexual differences and herbivory should consider resource investment across the entire growing and reproductive seasons due to the possible contrasting phenologies between male and female hosts (e.g., , , , ).
This study aimed to explore the relationships between galling insect attack and dioecy on three Baccharis species, mediated by investment in number of shoots, relative shoot growth rate and number of inflorescences. We assessed resource allocation to reproduction and growth between male and female plants along one year. The following questions were asked: i) Does resource allocation to reproductive and vegetative functions differ between male and female plants and, if so, does differential resource allocation provoke differences in gall attack between sexes? ii) Are there temporal differences in vegetative and reproductive investment between sexes and, if so, how such variations affect gall abundance across time?
Materials and Methods
Baccharis dracunculifolia DC is a widespread dioecious perennial shrub ranging from 2 to 3 m in height and is found in Brazil, Argentina, Uruguay, Paraguay and Bolivia . Baccharis ramosissima Gardner reaches from 2 to 4 m in height and occurs predominantly in Minas Gerais state, Brazil . Finally, Baccharis concinna Barroso is usually 1–2 m tall and is endemic to Serra do Cipó, Minas Gerais, Brazil . Fernandes et al.  recorded 17, 15, and 11 different gall-inducing species on B. dracunculifolia, B. concinna, and B. ramosissima, respectively. Each Baccharis species support a monophagous species of gall-inducing Baccharopelma . Baccharopelma dracunculiafoliae Burckhardt is the most common galling herbivore found on B. dracunculifolia, whereas Baccharopelma concinnae Burckhardt dominates the galling abundance on B. concinna, the same occurring for Baccharopelma brasiliensis Burckhardt on B. ramosissima (see ,  for details).
The populations of B. dracunculifolia and B. ramosissima were located in the Campus of the Universidade Federal de Minas Gerais, Belo Horizonte, Brazil (19° 30′ S, 44° 00′ W), at 805 m above sea level. The average temperature of the study site varies from 18°C to 20°C, and the average annual precipitation is 1,500 mm . The vegetation is extremely heterogeneous and disturbed, composed of native, introduced, ornamental and fruit-bearing species. The adjacent native vegetation contains dry forest and cerrado (savanna) species . The plants were all located in a 3 ha area at an early successional stage, dominated by B. dracunculifolia, B. ramosissima, grasses and herbaceous and shrubby leguminous species .
The population of B. concinna was situated at Serra do Cipó, in the Espinhaço Mountain Chain, approximately 100 km from Belo Horizonte. This region is characterized by quartzitic soils covered by rupestrian fields, highly xerophytic vegetation with predominance of herbs and shrubs . The study site was located at 1,250 m above the sea level (19° 17′ S, 43° 35′ W), inside a private protected area (Reserva Particular Vellozia). The climate is similar to the observed in Belo Horizonte, with average annual precipitation of approximately 1,500 mm and average temperature between 17.4–19.8°C .
Fourth-level shoots were counted every three weeks during one year.
Shoot measurements were performed every three weeks during one year (mean ± s.e.).
Thirty individuals (15 male and 15 female) of each Baccharis species were arbitrarily marked in field. Plant sex was determined by analysis of floral morphology (see  for details). We arbitrarily selected three secondary branches on each plant, which were tagged with thin, colored adhesive tape placed on their terminal 15 cm, and shoot length was measured every three weeks. Shoot relative growth rate was calculated as: [(Loge final length – Loge initial length)/(time final-time initial)] for each measurement date. Total number of inflorescences was counted for all marked shoots and inflorescence density was calculated by shoot centimeter. Both shoot growth and inflorescence densities were averaged per plant for statistical analyses. The number of fourth-level shoots was counted for each individual at the same intervals during one year (totaling 18 repeated measures). Fourth-level shoots are usually young and possess many sprouting leaves, thus this variable may be considered as an indication of the amount of active meristems in a given individual. Higher-level shoots (e.g., fifth and sixth level) are encountered only in few architecturally complex individuals and it would not be helpful to indicate meristem number for most of the plants sampled in this study . In B. dracunculifolia and B. concinna, phenological changes were recorded from May 2001 to May 2002, while in B. ramosissima, phenology was recorded from October 2001 to October 2002. In each sampling date, all live galls found on plants were counted without removal or marking. Thus, the same galls could have been occasionally counted during successive samplings. Galls were considered alive when exit holes from either the gall-inducer or parasitoids were absent from external walls. The present study considered only insect-induced galls .
To examine the intersexual differences in the number of fourth-level shoot, relative growth rate and inflorescence density along one year, we adjusted linear mixed-effect models (LME) for each of these response variables for each Baccharis species . These analyses were employed because the data were obtained repeatedly in the same plants during subsequent intervals, and the temporal autocorrelation generated by consecutive counting violates the assumption of sampling independence. Assuming independence when it is not true would inflate the error degrees of freedom and could lead to spurious significance (Type I error) . To overcome this problem, the data were grouped by plant and the error variances were calculated for each different group. In this case, the response is not the individual measure, but the sequence of measures in an individual . Plant sex, date and the interaction between sex and date were used as explanatory variables (fixed effects), whereas the resulting groups per plant were treated as random effects (date by plant identity).
To verify the effects of the date, sex and plant traits (number of fourth-level shoot, relative growth rate and inflorescence density) on gall abundance on the three Baccharis species, LMEs were also adjusted as described for the previous models. For these models, the response variables were log-transformed to meet normality. Minimal adequate models were adjusted with the removal of the non-significant terms. All model and analyses were conducted using the software R2.11 . All data are given as average ± standard error.
Resource Allocation to Growth and Reproduction
The phenological patterns exhibited by the three species of Baccharis were quite different (Figures 1, 2, 3). The number of fourth-level shoots varied significantly during the study period only for B. ramosissima, increasing after the end of the rainy season in March. Shoot number did not differ between male and female plants for all three species (Table 1, Figures. 1a, b, c). Although no significant temporal differences were observed for B. dracunculifolia, there was a peak on shoot growth for this species in November-December, at the beginning of the rainy season (Figure 2a). On the other hand, relative shoot growth rate varied significantly along the year for B. concinna and B. ramosissima. In B.concinna, higher growth occurred from November to February (rainy season) declining in March, with an additional peak in May (at the beginning of the dry season). The lowest growth rate was observed between July and October (dry season, Figure 2b). For B. ramosissima, a similar pattern was verified: higher shoot growth rate was observed during the rainy season (November-February), followed by a steady decline in March reaching almost zero between July and August (dry season, Figure 2c). Shoot growth rates were statistically similar between sexes for all species during this study (Table 1, Figures 2a, b, c).
The pattern of inflorescence production showed marked contrasts between the three Baccharis species (Figures 3 a, b, c). Inflorescence density varied significantly along the year for Baccharis dracunculifolia (Table 1), with two peaks of flowering: in the middle of the rainy season (December-January) and in the beginning of the dry season (April-May). Although the total inflorescence density did not differ between sexes, the temporal pattern of reproductive investment was sexually distinct for this species. In the first flowering peak, we observed a higher inflorescence density for the female gender, whereas in the second peak the opposite trend was detected (Figure 3a). Baccharis concinna presented inflorescences during the entire year, but the temporal pattern differed between sexes. For female plants, inflorescence density was slightly higher during the dry season, whereas males showed a peak in inflorescence production in the middle of the rainy season in January. As a whole, inflorescence density was constantly higher in female plants in this species (Table 1, Figure 3b). On the other hand, inflorescence density in B. ramosissima varied greatly during the year but did not differ between sexes (Table 1). For this species, inflorescences were produced only once a year, in the middle of the dry season (April-September) (Figure 3c). In general, the only plant trait considered in the present study that differed between sexes was the inflorescence density in B. concinna.
Sex-mediated Herbivore Attack
Baccharis dracunculifolia supported the highest richness (14 species) and abundance (20,327 counts) of insect galls among the studied host species. Both B. concinna and B. ramosissima showed 10 galling morphospecies each. Baccharis concinna individuals presented a total abundance of 8,353 insect galls whereas B. ramosissima supported 5,410 insect galls during the study period. Most of the galls were induced by psyllids belonging to the genus Baccharopelma (Hemiptera: Psyllidae). Baccharopelma dracunculiafoliae induced 80% of the galls on B. dracunculifolia, whereas Baccharopelma concinnae induced 65% of the galls on B. concinna, the same occurring for Baccharopelma brasiliensis on B. ramosissima (70% of the galls). The galls induced by the other insects had a very low relative abundance on the three Baccharis species.
Gall abundance showed different patterns of temporal variation for each Baccharis species (Table 2, Figure 4). Although no statistically significant difference in gall abundance was detected on B. dracunculifolia along time (Table 2), a bimodal pattern of attack was observed, with peaks in the transition between the rainy and dry season (April-June) and in the beginning of the rainy season (December) (Figure 4a). For B. concinna, gall abundance varied significantly along time (Table 2) and three peaks were recorded: in the middle of the dry season (July-August 2001), at the beginning (December 2001), and at the end (April 2002) of the rainy season. Significant temporal variations were also recorded for B. ramosissima, and the highest abundance of insect galls was observed in the rainy season (October-January) (Figure 4c).
Galls were counted every three weeks during one year.
Gall abundance did not differ between sexes in any of the three species of Baccharis (Table 2, Figure 4), not even in B. concinna which presented strong intersexual differences in inflorescence production. Also, the temporal pattern of gall attack was quite similar between male and female individuals in all host species during the entire study period (Figure 4). Gall abundance correlated positively with the number of fourth-level shoots on the three species of Baccharis (Table 2). Relative shoot growth rate also influenced positively the gall number on B. dracunculifolia and B. concinna (Table 2). Indeed, a higher gall abundance was recorded in the periods of greatest relative growth for all host species, although no significant relationship was detected in B. ramosissima (Table 2, Figures 2 and 4). Inflorescence density only affected positively the gall abundance on B. concinna, but this effect did not differ between sexes.
Resource Allocation to Growth and Reproduction
Plant sex does not seem to be an important source of variation in phenology and gall attack on Baccharis, corroborating previous studies in this speciose genus , , , , , . In spite of some intersexual differences in inflorescence production patterns in B. dracunculifolia and B. concinna, shoot number and growth rates were similar between genders in all three species. Thus, we found little support for the existence of differential resource allocation to growth and reproduction between sexes. In dioecious species, male and female individuals have different reproductive functions (i.e., pollen donation vs. seed production and fruit maturation) and, in many cases, fleshy fruits and nutrient-rich seed structures are energy-costly , –, thus leading to strong asymmetries in reproductive investment between sexes. In Baccharis, inflorescences are small and comparable in size between male and female individuals of all three species, and seeds are tiny and wind-dispersed . Thus, female plants probably do not have a reproductive investment greater enough than males to produce differences in meristem number and plant growth.
In the case of B. concinna, females indeed produced a significantly higher number of inflorescences than males. However, this species is wind-pollinated  and has the smallest inflorescences among the three species studied here. This differential investment did not affect plant growth and shoot production, which remained roughly similar for both sexes during the entire year, suggesting the absence of trade-offs in this species (i.e., male B. concinna individuals had both highest growth rate and inflorescence density early in the rainy season, in December-February). Similarly, Antos and Allen  did not find a significant correlation between reproductive effort and growth in either sex of the shrub Oemleria cerasiformis (Rosaceae). Temporal variations in inflorescence production may represent a response to other factors that regulate reproductive investment (e.g. availability of light and soil nutrients and moisture) , , . Thus, the small wind-pollinated inflorescences of B. concinna are likely relatively inexpensive in terms of resource allocation and affordable throughout the year for both genders.
For B. dracunculifolia, the timing of reproduction was different between sexes. Although male plants started reproduction earlier, females bloomed in January, in the peak of the rainy season, and this massive investment probably limited their inflorescence production for the rest of the season. The low reproductive male investment in January may have been caused by their higher resource allocation to growth in the previous month, saving energy for inflorescence production later in the season (see Figure 2a). The pattern detected here was opposite to the observed by Espírito-Santo et al. , who reported a higher male investment early in the season related to pollinator attraction, and a late female reproductive investment due to seed maturation (especially in June). However, this previous study was much more limited in time (March-June), and there were strong inter-annual variations in reproductive investment of B. dracunculifolia (i.e., no inflorescences in June in the present study). Thus, our results suggest that sexual differences in phenology may not be determined only by male and female reproductive functions (pollen donation vs. seed production and fruit maturation), and are also affected by abiotic (e.g., water availability) and biotic (e.g., interspecific competition for pollinators) pressures. In this way, long-term studies are necessary to detect temporal patterns in reproductive investment in dioecious plants.
For the studied Baccharis species, the majority of changes in phenological events was not related to plant sex, but varied between species and is likely associated with resource availability in each plant population. In B. dracunculifolia and B. concinna, the higher number of fourth-level shoots and relative shoot growth rate occurred during the rainy season. In contrast, B. ramosissima produced new branches and flowers in the dry season, when shoot growth rate was reduced. Thus, this species may have not been able to sustain growth and reproduction simultaneously, especially in periods of resource scarcity. In B. ramosissima, inflorescences of both sexes are similar in size and pollination is performed by social bees . Blooming in the middle of the dry season is not unusual in some tropical environments, since the low air humidity contributes to increase nectar concentration and leaf fall may improve flower visibility by pollinators . In the case of B. ramosissima, it is likely that this strategy reduces competition for pollinators with the sympatric B. dracunculifolia. These two species are very similar in architecture  and in inflorescence morphology. Since B. ramosissima populations are usually much less dense when co-occurring with B. dracunculifolia (M. M. Espírito-Santo, personal observation), it is likely that there was a strong evolutionary pressure for niche differentiation causing both sexes of B. ramosissima to reproduce very synchronously during periods of water scarcity but high pollinator availability.
Sex-mediated Herbivore Attack
The gall abundance on the studied species of Baccharis did not conform to the pattern predicted by the sex-biased herbivory hypothesis. On the other hand, this result corroborates several other studies related to this genus in Brazil , , , , . Furthermore, the present study involved multiple hosts and galling species, and closely followed temporal changes in gall abundance and host resource availability along one year. Therefore, we have consistent support to reject the male-biased attack prediction of the sex-biased herbivory hypothesis.
The absence of the gender-related gall attack is explained by the lack of differences in plant growth rate and meristem availability (estimated indirectly by the number of fourth-level shoots) between male and female plants. Indeed, meristem number was already reported as the most important plant trait driving gall abundance and species richness across several species of Baccharis . This assertion is reinforced by the positive relationship between gall abundance and shoot number and plant growth rate in the present study. However, other plant traits not evaluated here such as nutrient content, secondary chemistry and physical defenses can differ between sexes and affect gall attack (see ). Although we did not quantify plant defenses, there is some evidence that carbon-based compounds such as tannins are not abundant and do not vary between sexes in B. dracunculifolia , , but nothing is yet known for B. concinna and B. ramosissima. Furthermore, gall-inducing insects are capable of manipulating chemical composition, growth rates and developmental patterns of the attacked plant organ , , , and are able to overcome chemical and physical defenses regardless of plant gender. Thus, the similarities in gall abundance between male and female individuals in this host genus are likely related to plant vigor and availability of young tissues for gall induction, but leaf nutrients and defenses deserve further investigation.
Gall abundance was influenced by plant phenology, being mainly affected by temporal variations in shoot growth rates. Indeed, gall attack was higher mostly during the rainy season, when shoot growth was more vigorous in all three species. Although the number of fourth-level shoots was the most important plant trait affecting gall abundance at the individual level, statistically significant temporal variations in this variable were only detected in B. ramosissima. However, shoot production in this species increased during the dry season and may be related to flowering, when shoot growth and gall attack were both low. For B. dracunculifolia, other studies detected an increase in the number of fourth-level shoots and galls in March-April, right before the second peak of flowering , . However, such phenological pattern was only observed for male plants of this species in the present study, and a higher gall attack did not track shoot number. Thus, it seems that plant growth rates are more important in determining temporal variations in gall attack, whereas the number of fourth-level shoots is responsible for spatial, individual differences in plant susceptibility.
Overall, the Baccharis species studied here did not exhibit sexual dimorphisms on vegetative traits that are usually related to distinct reproductive functions. The absence of differential reproductive allocation may have contributed to similar growth and shoot production between male and female plants. As a bottom-up consequence, plant gender is a weak predictor, or even an irrelevant variable influencing gall abundance in this host plant genus. In spite of that, the plant traits evaluated here are important drivers of gall attack both in space (between individuals) and time, and temporal changes in gall abundance are synchronized with resource availability (e.g. young tissues and vigorous growth) throughout the year. The absence of sex-biased herbivory can be more frequent than usually reported, since studies that fail to detect intersexual variation on herbivore attack in other system are probably underrepresented in the literature.
We are very grateful to CF Sperber, CM Jacobi, FAO Silveira, KD Floate, SP Ribeiro, TG Cornelissen, and two anonymous reviewers for their valuable suggestions on earlier versions of the manuscript, and to FR Andrade-Neto, R Pereira and BG Pacheco for field assistance.
Conceived and designed the experiments: MMES GWF. Performed the experiments: MMES FSN. Analyzed the data: MMES JOS. Contributed reagents/materials/analysis tools: JOS MMES. Wrote the paper: MMES JOS FSN GWF.
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