Epstein-Barr virus (EBV) is an important environmental factor associated to the development of Burkitt lymphoma (BL) in endemic and intermediate risk regions. However, little is known about the contribution of genetic constitution to the development and clinical response of the disease. The aim of this work was to investigate the role of EBV and Interleukin 10 (IL10) single nucleotide polymorphisms (−1082A/G, −819C/T, −592C/A) and microsatellites (IL10.R and IL10.G) in susceptibility and clinical outcome in pediatric BL patients, in a region with intermediate EBV association frequency. The frequencies of IL10 promoter Single nucleotide polymorphisms −1082A/G, −819C/T, −592C/A, and IL10.R and IL10.G microsatellites were compared in 62 pediatric patients and 216 healthy donors. IL10 −1082GG and GCC/GCC genotypes were more frequent in patients than in controls, and associated to a higher risk of BL development (GG genotype OR 2.62, 95% CI, 1.25–5.51; P = 0.008; Pc = 0.024). EBV was detected in tumor samples by EBER-ISH in 54.1% of cases. EBV+ patients exhibited a better event free survival (EFS) (P = 0.019) than EBV− patients. Carriers of IL10 R3-GCC had worse EFS (P = 0.028). Our results suggest a risk effect and an independent prognostic value of IL10 polymorphisms and EBV in childhood BL patients.
Citation: Minnicelli C, Barros MHM, Klumb CE, Romano SO, Zalcberg IR, Hassan R (2012) Relationship of Epstein-Barr Virus and Interleukin 10 Promoter Polymorphisms with the Risk and Clinical Outcome of Childhood Burkitt Lymphoma. PLoS ONE 7(9): e46005. https://doi.org/10.1371/journal.pone.0046005
Editor: Luwen Zhang, University of Nebraska - Lincoln, United States of America
Received: May 10, 2012; Accepted: August 27, 2012; Published: September 27, 2012
Copyright: © Minnicelli et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by the Swissbridge Foundation (Switzerland), Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ, PensaRio) and Instituto Nacional de Ciência e Tecnologia (INCT) para Controle do Câncer (Grants Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq 573806/2008-0 and FAPERJ E26/170.026/2008) (Brazil). Sponsors had no involvement in the study design, in the collection, analysis and interpretation of data, in the writing of the manuscript, or in the decision to submit the manuscript for publication.
Competing interests: The authors have declared that no competing interests exist.
Burkitt lymphoma (BL) is an aggressive non-Hodgkin lymphoma (NHL) of mature B cells which varies in respect to age-specific incidence, primary site of tumor and Epstein-Barr virus (EBV) association in different geographic situations , . These differences allowed for the characterization of the endemic (eBL) and sporadic (sBL) subtypes; the former affecting young African children, with involvement of abdomen and facial bones, and the latter occurring worldwide, mainly affecting young adults, with involvement of the terminal ileum and lymph nodes , . Respective to infection status, eBL is almost always EBV-associated while sBL shows a more irregular association, ranging from 10% to 30% EBV+ cases in different geographical areas , . In Brazil, BL is mainly a childhood disease, and EBV association ranges from 80% in Northeast  to 50–60% in the Southeast region of the country –. We have previously proposed an intermediate risk status for childhood BL arisen in South-East Brazil , , where a causal relation between age of EBV seroconversion and BL risk was suggested. However, the exact role of EBV in BL pathogenesis or disease progression has been elusive until now .
MYC oncogene deregulation resulting from its translocation to one of the immunoglobulin genes is the common pathogenic event for all BL subtypes, which can account for most of the cellular disease features . However, the causes of BL epidemiological and clinical variability are hidden by the complexity of environmental interactions that might act in different geographical situations where the tumor arises. Host factors influencing BL genetic risks are largely unknown, which prompted us to investigate the potential involvement of interleukin 10 (IL10) gene polymorphisms in disease susceptibility and clinical response.
IL10 is part of an immunoregulatory cytokine network, produced mainly by monocytes, macrophages, T cells, and normal and neoplastic B cells . Besides the immunosuppressor effects mediated by this cytokine, which may support an environment favorable to neoplastic cells expansion , IL10 can also exert proliferative effects on B cells, particularly in BL cells, acting as a paracrine or autocrine proliferative factor .
Familiar and twin studies showed that 50–75% of inter-individual variability in IL10 levels can be attributed to genetic variations , . Three Single Nucleotide Polymorphisms (SNPs), −1082(A/G), −819(C/T) and −592(C/A), localized in the proximal gene promoter, exert a functional effect on IL10 expression . These polymorphic sites are in linkage disequilibrium (LD) and form haplotype families when combined to microssatellites IL10.G (−1.2 Kb) and IL10.R (−4 Kb) .
Being IL10 an indispensable B-cell survival factor, it is not surprising that EBV has developed mechanisms to coopt its biological properties. EBV is capable of inducing human IL10 in B cells through its viral RNAs , . Moreover, it encodes a viral IL10 (vIL-10), which is ∼80% homologue and shares many of the biological properties of the human cytokine . vIL10 has important roles in inducing B cell growth and tolerizing the immune responses during pre-latent phase or lytic reactivaction , . Reinforcing the role of this cytokine in EBV infection, human IL10 polymorphisms were associated to the timing and susceptibility of primary EBV infection in a healthy Finland population , . In the light of the demonstrated biological interactions between IL10, BL and EBV infection, a case-control study was performed to investigate the role of IL10 gene promoter polymorphisms in BL pathogenesis, as well as in the therapeutic response, in a region with intermediate EBV association frequency.
This study was approved by the Instituto Nacional de Câncer (INCA) Ethics Committee, and has therefore been performed in accordance with the ethical standards of the Declaration of Helsinki and the Brazilian rule 196/96 of the Conselho Nacional de Saúde. All patients and controls were included in the study after written informed consent. Guardians have signed the informed consent on the behalf of the children participants involved in this study.
Patients and Pathological Samples
Sixty-two children (up to 16 years old), diagnosed with Burkitt lymphoma/leukemia at the Instituto Nacional de Câncer (INCA), Rio de Janeiro, Brazil, were included in this study, based on availability of pathology specimens and clinical records.
Histopathological diagnosis was based on morphological criteria and immunohistochemical detection of CD20 and CD10 and a high proliferation rate (Ki-67 almost 100%), according to the WHO classification . Samples consisted of fresh biopsies and/or bone marrow cells in 50 cases, and formalin-fixed paraffin embedded tissues (FFPET) in the remaining twelve. Disease staging was performed according to the St. Jude's/Murphy staging classification for childhood Non-Hodgkin lymphoma .
Intensity of treatment was adjusted to risk groups (RG). Patients in risk group 1 (RG1) presented with St Jude's stage I or II, and one completely resectable lymphomatous lesion. RG2 patients presented with non-resectable extra-abdominal primary disease or stage II abdominal tumor with lactic acid dehydrogenase (LDH) augmented less than two times the normal levels.
High risk disease (risk group 3, RG3) included patients with abdominal tumor (St. Jude stage III), LDH up to 1000 U/l, involvement of BM or central nervous system or multimodal bone disease (St. Jude stage IV), as well as patients classified as L3 acute lymphoblastic leukemia . All children were treated according to the NHL-Berlin-Frankfurt-Munster (BFM) protocols backbone strategies, and among them, 39 were uniformly treated with the LNH-98 INCA institutional protocol .
The control group included 63 children (1–16 years; median 11) and 153 adults (17–61 years; median 36), totalizing 216 healthy individuals from the same geographic region (Rio de Janeiro State), registered for bone marrow or peripheral blood donation at Bone Marrow Transplantation Center (CEMO – INCA), a public institution offering hematopoietic stem cell transplantation to the National Health System patients in Rio de Janeiro, Brazil. Sex ratio between male and female was 2.2∶1.
Donors belonged to a comparable socio-economic and ethnic origin as BL patients, since they were selected from the relatives of transplant candidates at our hospital in the same period of the present study, excluding lymphoma patient relatives. Ethnic matching of patients and controls was not taken into account in the experimental design since relevant studies showed that skin color or self referred ethnic origin are not good markers of ancestry in Brazil . Moreover, previous comparisons of IL10 gene polymorphisms between normal Afro- and Euro-descendents persons from the same geographical region (Rio de Janeiro State) and African and European populations did not disclose any ethnical stratification in Brazilians .
EBV was detected by RNA in situ hybridization, performed on FFPET sections using fluorescein isothiocyanate (FITC)-conjugated EBV-encoded RNAs (EBERs) oligonucleotides probes (EBER-ISH). Detection of hybridized sites was achieved using a monoclonal antibody anti-FITC labeled with alkaline phosphatase (Novocastra Laboratories Ltd., Newcastle upon Tyne, UK).
High molecular weight DNA was obtained from fresh samples (biopsies, bone marrow or peripheral mononuclear cells) with standard procedures. FFPET-DNA was extracted from 5 µm-sections, following strict measures for avoiding cross-contamination between samples, as described .
IL10 SNPs −1082A/G and −592C/A were detected by Allele-specific (AS-) PCR, as described in Supplementary Table 1. SNP −592 (rs1800872) genotyping results were independently confirmed by using a TaqMan® allelic discrimination pre-developed assay (C_1747363_10) (Applied Biosystems, Foster City, CA, US), in an ABI PRISM 7700 Sequence Detector (Applied Biosystem), to confirm results obtained with conventional PCR methods.
Based on the strong linkage disequilibrium between SNPs −819 and −592, three crossing AS-PCRs were simultaneously carried out using combinations of primers at positions −1082A/G, −819C/T and −592C/A, to experimentally confirm the haplotypes ACC, ATA and GCC (Table S1).
Alleles of microssatellites IL10.R and IL10.G (CA)n were amplified with fluorescence labeled oligonucleotides, as previously described  and PCR fragments were analyzed in an automatic sequencer (MegaBACE 1000 DNA Sequencer; Amersham Pharmacia Biotech, Piscataway, NJ, US) with the Gene Profiler software.
All PCR reactions were carried out with final volumes of 30 µl containing 0.2 mM of each dNTP, 0.2 µM of each primer and 0.5 U of Platinum Taq DNA Polymerase, (Invitrogen, Carlsbad, CA, USA), from 50 ng of genomic DNA.
Positive and negative controls and 140 replicate samples were interspersed in the genotyping assays. Agreement for quality control replicates and duplicates was more than 98% for all assays.
Allele frequencies were calculated by the allele counting method. Hardy-Weinberg equilibrium was tested for each SNP by comparing observed with expected frequencies using a χ2 test. Three different genetic models were tested, including dominant model (comparing homozygous wild-type genotype with variant allele carrying genotypes), recessive (comparing wild-type allele-carrying genotypes with homozygous variant genotype), and additive model (Cochran-Armitage test). The best-fitting among the three models was the one with the smallest p value. The program R (R Development Core Team, 2006, http://cran.r-project.org) was used for genetic modeling, using a specific script developed for the purpose of the case-control analyses. Associations between polymorphisms and pathologic condition were tested using Fisher's exact and Pearson's χ2 tests. Magnitude of associations was calculated as odds ratios (OR) with 95% confidence interval (CI) by unconditional logistic regression analyses.
Event free survival (EFS) was the interval (in months) from diagnosis to progression at any time, relapse from complete response, or initiation of new, previously unplanned treatment or to the last follow-up in the patients with treatment success. EFS probabilities were estimated by the Kaplan-Meier method and compared by log-rank test. The multivariate Cox proportional hazard regression method was used to determine the independent prognostic value of statistically significant variables in univariate analyses, adjusted by sex and age when necessary. Data were analyzed using Statistical Package for the Social Sciences 13.0 (SPSS) and P values<0.05 were considered statistically significant.
The patient's group was characterized by a predominance of male (M43∶ F19, sex ratio 2.26) and very young children (median age 5 years, 2–14). Complete clinical information was available for 54 children, with 38 (70.3%) being in advanced disease stages (III/IV) and 32 (59.3%) in RG3. Demographic and clinical data of patients are shown in Table 1.
EBV was detected in 33 of 61 (54.1%) cases tested. A relationship between lower age and EBV infection was observed (P = 0.008, Fisher's exact test).
Genotype and Allele Frequencies of IL10 Promoter
Comparisons between adults and children in the control group revealed no difference regarding genotype/haplotype frequencies (Table S2); therefore, the entire control group was used in the case-control analysis.
A comparison of the genotype and allele frequencies of IL10 −1082A/G and −592C/A polymorphisms between cases and controls is shown in Table 2. All genotype frequencies were in Hardy-Weinberg equilibrium in both groups (BL χ2 1.13, P = 0.28; Controls, χ2 0.03; P = 0.85 for −1082A/G and BL χ2 0.016, P = 0.89; Controls, χ2 0.004; P = 0.94 for −592C/A). The −1082GG genotype was overrepresented in patients (23% vs 10.2%), associated to a higher risk of BL development (OR 2.62, 95% CI, 1.25–5.51; P = 0.008). Significance of the genotype imbalance held true after Bonferroni correction for multiple comparisons (two polymorphisms and 3 genetic models, Pc = 0.024) In agreement, the haplotype combination GCC/GCC was also associated with BL risk (OR 2.53, CI 95% 1.2–5.32, P = 0.012; Pc = 0.072). No differences between cases and controls were found for −592C/A allelic or genotypic frequencies (Table 2).
No statistical differences were observed in IL10 polymorphisms frequencies between EBV+ and EBV− subgroups (Fig. 1).
IL10.G and IL10.R were analyzed in 462 chromosomes (102 BL and 360 controls). IL10.G alleles varied between G6 and G14; (CA)18 to (CA)26. Microssatellite IL-10.R alleles ranged from R1 to R4; (CA)8, to (CA)11. No differences were observed between patients and controls for IL10.G and IL10.R alleles (P = 0.47 and P = 0.54, respectively, Monte Carlo simulation with 1000 bootstraps, CI 99%).
IL10 Haplotype Families
IL10 haplotype families were built according to Eskdale et al, 1999 , as follows: family IL10.01: IL10.R3-G-C-C; IL10.02: R2-A-C-C; IL10.03: R2-G-C-C; and IL10.04: R2-A-T-A. Three rare families were recorded both in patients and controls (Table 3). No statistical differences were observed in the frequencies of IL10 families between patients and controls (102 and 360 chromosomes, respectively) (P = 0.165).
Survival of BL Patients
Thirty nine patients received the same treatment and supportive care  and these were evaluated for OS and EFS, with a follow-up period ranging from 1 to 190 months (median 82 months). The EFS of the whole group was 79.5%. Risk group, despite not statistically significant, was the clinical characteristic that best distinguished patients with good and bad outcome (RG2 92.9% vs. RG3 69.6%, P = 0.11; Log-rank test).
EBV status was associated with therapy response, with a better EFS observed in the EBV+ compared to the EBV− patients (91.3% vs. 60%, P = 0.019, Fig. 2A). These differences reflected the prognostic value of EBV in the RG3 (EFS 91.7% vs 45.5%, P = 0.014; EVB+ and EBV−, respectively) compared to RG2 (90.9 vs. 100%, respectively; P = 0.54).
When IL10 polymorphisms were analyzed in respect of clinical response, the presence of IL10.01 family (R3-GCC) was related to a poor outcome (EFS 50% vs. 83.3%, P = 0.028, Fig. 2B). Again, these differences reflected the prognostic value of IL10.01 in RG3 (25% for IL10.01 carriers vs. 75% for non-carriers, P = 0.017), compared to RG2 (75% for carriers vs. 100% for non-carriers; P = 0.18).
As for the microsatellite IL10.G, when each allele was analyzed separately, allele G12 was related to a poor EFS (55.6% vs. 85.2%, P = 0.055) with a significant statistical impact in patients in RG3 (33.3% vs. 81.3%, P = 0.017) compared to RG2 (100% vs. 90.0%, P = 0.65, Fig. 2C). None of the other IL10 promoter polymorphisms were associated to outcome. Multivariate Cox analyses confirmed the independent prognostic value of EBV, IL10.01 and IL10.G12 (Table 4).
When patients were grouped according to the presence of these two prognostic factors, patients EBV+/IL10.01− showed the best EFS (92.9%), while patients EBV−/IL10.01+ showed the worst EFS (25%); intermediate to these groups were the patients with the presence of one of the good prognostic factors, EBV+/IL10+ (75%) or EBV−/IL10.01− (66.7%), (P = 0.01). The statistical significance of this model augmented when stratified for RG3 (P = 0.001; Fig. 2D). None of the survival comparisons were affected by patients' sex and age.
The role of environmental factors in the pathogenesis of BL has been largely studied, mainly in the endemic form of the disease, in which malaria, age of EBV infection, and nutrition status, among others, have been implicated , . EBV is an important environmental factor associated to ∼50% of BL cases in our region, with a proposed role for early EBV primary infection in the pathogenesis of the young BL cases . However, little is known about the genetic constitution of people affected by the disease at individual and population levels. In the present study a higher than expected frequency of IL10 −1082GG genotype and GCC haplotype was detected in BL patients, associated to an approximately 2.5-fold augmented risk of lymphoma development, suggesting that IL10 functional variants could contribute to the genetic context in which BL develops. In addition, the analysis of a uniformly treated group of patients showed that the presence of EBV in tumor cells was associated with a favorable outcome, especially in the advanced risk group, while IL10.01 family and IL10.G12 allele were associated to an unfavorable outcome.
Despite the well described interactions between EBV and IL10, in this study we did not observe significantly different frequencies of IL10 polymorphisms in EBV+ or EBV− groups. Since EBV-associated BL in our region, as well as in the African endemic subtype, affects very young children, it is possible that IL10 (either human or viral) plays a more complex role in age-specific risks associated to EBV primary infection, which cannot be addressed with the design of the present study.
The prognostic value of EBV in BL has been investigated in a few studies, since a proper approach to this question is prevented mainly because of the low frequency of EBV+ or EBV− cases seen in sporadic or endemic areas, respectively. With a similar number of EBV+ and EBV− cases originated from the same geographical region, we have been able to disclose a favorable effect of EBV on the rate and duration of EFS in our pediatric BL series. A recent work from a similar epidemiologic setting of Brazil (São Paulo State) showed also a better, albeit non significant outcome for EBV+ BL cases . It is likely that the failure to find a significant statistical association of the above mentioned study was due to the inclusion of patients treated with two different protocols, over a long period, along which improvement of support conditions could have influenced survival of the overall group. This was indeed the reason of having selected a uniform group of patients to investigate the prognostic value of EBV and IL10 genetic variants, since differences in outcomes of BL patients treated with the same protocol before and after 1999 were observed in our institution .
At present, we do not have an explanation for the association of EBV with a better therapeutic response. In a group of children diagnosed with classical Hodgkin lymphoma in the same period from the same institution, we have also observed a favorable outcome associated with the presence of EBV in tumor Hodgkin-Reed Sternberg cells . It is possible that in children, where EBV tumor association may be a late complication of EBV primoinfection, clinical behavior might be associated with a differential immune response to viral antigens, as seems to be reflected in the microenvironment composition of pediatric cHL . Conversely, in EBV-associated adult lymphomas, impaired immunosurveillance might be at the root of the pathogenesis and of the unfavorable therapeutic response observed in this clinical setting –. In our patients a particularly favorable response was observed in EBV+ high-risk BL patients. One possible, yet unproven, explanation is that a high tumor burden would be associated with an augmented rate of spontaneous lytic entry in the EBV+ cases, which would potentiate chemotherapy effects. In fact, in endemic BL, where EBV is present in virtually 100% of cases, the expression of the lytic transactivactor Bzlf1 (Zebra) in BL cells was shown to be associated with a favorable response to treatment . At present, promising protocols to treat EBV-associated cancers are based on the induction of EBV lytic or latent expression patterns, aiming to modify phenotypic characteristics and collaborate in the lysis of tumor cells –.
To our knowledge, this is the first study to correlate IL10 genetic variations with susceptibility and therapeutic response in a homogeneous series of pediatric BL. In other B-cell NHL, large studies identified IL10 polymorphisms as significant risk factors for specific groups of NHL, such as diffuse large B-cell lymphoma –. However, association of specific IL10 genetic variants with therapeutic response has not been clearly demonstrated , .
Despite some controversies, most authors agree with an association of −1082G allele in homozygosis with high IL10 expression , , , . Specific microsatellite alleles, such as IL10.R2 and G12 were also shown to be associated with higher IL10 levels . Thus, the role of IL10 in BL pathogenesis might probably be related to the stimulation of B lymphocytes proliferation, coupled with the antiapoptotic properties of this cytokine , . Moreover, in BL, the production of IL10 by tumor cells might stimulate intratumoral macrophages to differentiate and to promote B cell survival . Therefore, the main effector of the inter-individual differences of IL10 expression, either B cells or macrophages, is at present undefined.
Regarding therapeutic response, the presence of IL10.01 family and IL10.G12 allele were associated to an unfavorable outcome. The haplotype family (IL10.01) includes the component GCC related to a high IL10 production , , , what was in fact identified as a risk factor in our case-control study. IL10.G12 is among alleles (G10, G12 e G14) also associated to the induction of high levels of IL10 .
We are aware of the limitations imposed to our results by the lack of statistical power of our sample number, the need of future studies to replicate our case-control results and the establishment of the mechanisms responsible for the effects of EBV and IL10 on BL clinical response. Besides, we were not able to assert that cases and controls are similar in respect of the genetic background since we have not ethnically matched them. In that regard, the fact that the patients shared the same socio-geographical origin than controls may help mitigate the effect of a potential genetic sub-structure in this study. Moreover, the inter-ethnic admixture of the Brazilian population, among Europeans, Africans and Amerindians, is depicted in genetic analysis as varying over wide ranges in a continuum across the categories of self-reported ethnicity , . This view of the genetic variations of Brazilians supports strategy of control selection based on biogeographical ancestry instead of race or color, as claimed for pharmacogenetic studies in Brazil .
In sum, with modern intensive chemotherapy regimens, BL is cured in about 80–90% of cases . However, early relapse and refractoriness are an actual limitation of BL treatment, due to the lack of therapeutic options. Identification of biological markers for risk stratification, mainly in the high risk patients, is a current goal in BL research. Our preliminary results indicate that EBV and IL10 polymorphisms are worth to further investigation in pediatric BL, as they may represent useful clinical biomarkers.
Technical information about IL10 genotyping PCR assays.
Comparisons of genotype, haplotype and family frequencies between children and adult controls.
Conceived and designed the experiments: CM RH. Performed the experiments: CM MHMB. Analyzed the data: CM MHMB CEK IRZ RH. Contributed reagents/materials/analysis tools: CEK SOR. Wrote the paper: CM RH.
- 1. Magrath I, Jain V, Bhatia K (1992) Epstein-Barr virus and Burkitt's lymphoma. Semin Cancer Biol 3: 285–295.
- 2. Swerdlow SH, Campo E, Harris NL, Jaffe ES, Pileri SA, et al.. (2008) International Agency for Research on Cancer, World Health Organization: WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. 4th ed. Lyon, France: IARC Press.
- 3. Hsu JL, Glaser SL (2000) Epstein-Barr virus-associated malignancies: epidemiologic patterns and etiologic implications. Crit Rev Oncol Hematol 34: 27–53.
- 4. Araujo I, Foss HD, Bittencourt A, Hummel M, Demel G, et al. (1996) Expression of Epstein-Barr virus-gene products in Burkitt's lymphoma in Northeast Brazil. Blood 87: 5279–5786.
- 5. Hassan R, Klumb CE, Felisbino FE, Guiretti DM, White LR, et al. (2008) Clinical and demographic characteristics of Epstein-Barr virus-associated childhood Burkitt's lymphoma in Southeastern Brazil: epidemiological insights from an intermediate risk region. Haematologica 93: 780–783.
- 6. Queiroga EM, Gualco G, Chioato L, Harrington WJ, Araujo I, et al. (2008) Viral studies in Burkitt lymphoma: association with Epstein-Barr virus but not HHV-8. Am J Clin Pathol 130: 186–192.
- 7. Pizza M, Bruniera P, Luporini SM, Marcelino da Silva HR, Borsato ML, et al. (2008) Detection of Epstein-Barr virus in children and adolescents with Burkitt's lymphoma by in situ hybridization using tissue microarrays. Hematology 13: 114–118.
- 8. Klumb CE, Hassan R, De Oliveira DE, De Resende LM, Carriço MK, et al. (2004) Geographic variation in Epstein-Barr virus-associated Burkitt's lymphoma in children from Brazil. Int J Cancer 108: 66–70.
- 9. Thorley-Lawson DA, Allday MJ (2008) The curious case of the tumour virus: 50 years of Burkitt's lymphoma. Nat Rev Microbiol 6: 913–924.
- 10. Hecht J, Aster J (2000) Molecular biology of Burkitt's lymphoma. J Clin Oncol 18: 3707–3721.
- 11. Moore KW, de Waal Malefyt R, Coffman RL, O'Garra A (2001) Interleukin-10 and the interleukin-10 receptor. Annu Rev Immunol 19: 683–765.
- 12. Holland G, Zlotnik A (1993) Interleukin-10 and cancer. Cancer Invest 6: 751–758.
- 13. Guedez L, Mansoor A, Birkedal-Hansen B, Lim MS, Fukushima P, et al. (2001) Tissue inhibitor of metalloproteinases 1 regulation of interleukin-10 in B-cell differentiation and lymphomagenesis. Blood 97: 1796–1802.
- 14. Westendorp RG, Langermans JA, Huizinga TW, Elouali AH, Verweij CL, et al. (1997) Genetic influence on cytokine production and fatal meningococcal disease. Lancet 349: 170–173.
- 15. Reuss E, Fimmers R, Kruger A, Becker C, Rittner C, et al. (2002) Differential regulation of interleukin-10 production by genetic and environmental factors-a twin study. Genes Immun 3: 407–413.
- 16. Turner DM, Williams DM, Sankaran D, Lazarus M, Sinnott PJ, et al. (1997) An investigation of polymorphism in the interleukin-10 gene promoter. Eur J Immunogenet 24: 1–8.
- 17. Eskdale J, Keijsers V, Huizinga T, Gallagher G (1999) Microsatellite alleles and single nucleotide polymorphisms (SNP) combine to form four major haplotype families at the human interleukin-10 (IL-10) locus. Genes Immun 1: 151–155.
- 18. Burdin N, Péronne C, Banchereau J, Rousset F (1993) Epstein-Barr virus transformation induces B lymphocytes to produce human interleukin 10. J Exp Med 177: 295–304.
- 19. Kitagawa N, Goto M, Kurozumi K, Maruo S, Fukayama M, et al. (2000) Epstein-Barr virus-encoded poly(A)(-) RNA supports Burkitt's lymphoma growth through interleukin-10 induction. EMBO J 19: 6742–6750.
- 20. Jochum S, Moosmann A, Lang S, Hammerschmidt W, Zeidler R (2012) The EBV immunoevasins vIL-10 and BNLF2a Protect newly infected B cells from immune recognition and elimination. PLoS Pathog 8: e1002704.
- 21. Helminen M, Lahdenpohja N, Hurme M (1999) Polymorphism of the interleukin-10 gene is associated with susceptibility to Epstein-Barr virus infection. J Infect Dis 180: 496–499.
- 22. Helminen ME, Kilpinen S, Virta M, Hurme M (2001) Susceptibility to primary Epstein-Barr virus infection is associated with interleukin-10 gene promoter polymorphism. J Infect Dis 6: 777–780.
- 23. Murphy SB (1980) Classification, staging and end-results of treatment of childhood non-Hodgkin's lymphoma - Dissimilarities from lymphoma in adults. Semin Oncol 7: 332–339.
- 24. Klumb CE, Schramm MT, De Resende LM, Carriço MK, et al. (2004) Treatment of children with B-cell non-Hodgkin's lymphoma in developing countries: the experience of a single center in Brazil. J Pediatr Hematol Oncol 26: 462–468.
- 25. Parra FC, Amado RC, Lambertucci JR, Rocha J, Antunes CM, et al. (2003) Color and genomic ancestry in Brazilians. Proc Natl Acad Sci USA 100: 177–182.
- 26. Moraes MO, Santos AR, Schonkeren JJ, Vanderborght PR, Ottenhoff TH, et al. (2003) Interleukin-10 promoter haplotypes are differently distributed in the Brazilian versus the Dutch population. Immunogenetics 54: 896–899.
- 27. Stefanoff CG, Hassan R, Gonzalez AC, Andrade LA, Tabak DG, et al. (2003) Laboratory strategy for efficient handling of paraffin-embedded tissues for molecular detection of clonality in non-Hodgkin lymphomas. Diag Mol Pathol 1: 279–287.
- 28. Kube D, Mörmann M, Tomiuk J, Rieth H, Hua TD, et al. (2003) Simultaneous analysis of interleukin-10 gene microsatellites and single-nucleotide polymorphisms in parallel with tumour necrosis factor and interferon-gamma short tandem repeats by fluorescence-based polymerase chain reaction. Genes Immun 4: 459–468.
- 29. Sumba PO, Kabiru EW, Namuyenga E, Fiore N, Otieno RO, et al. (2010) Microgeographic variations in Burkitt's lymphoma incidence correlate with differences in malnutrition, malaria and Epstein-Barr virus. Br J Cancer 103: 1736–1741.
- 30. Barros MH, Vera-Lozada G, Soares FA, Niedobitek G, Hassan R (2011) Tumor microenvironment composition in pediatric classical Hodgkin lymphoma is modulated by age and Epstein-Barr virus infection. Int J Cancer 15;18: 3762–3771.
- 31. Keegan TH, Glaser SL, Clarke CA, Gulley ML, Craig FE, et al. (2005) Epstein-Barr virus as a marker of survival after Hodgkin's lymphoma: a population-based study. J Clin Oncol 23: 7604–7613.
- 32. Diepstra A, van Imhoff GW, Schaapveld M, Karim-Kos H, van den Berg A, et al. (2009) Latent Epstein-Barr virus infection of tumor cells in classical Hodgkin's lymphoma predicts adverse outcome in older adult patients. J Clin Oncol 27: 3815–3821.
- 33. Oyama T, Yamamoto K, Asano N, Oshiro A, Suzuki R, et al. (2007) Age-related EBV-associated B-cell lymphoproliferative disorders constitute a distinct clinicopathologic group: a study of 96 patients. Clin Cancer Res 13: 5124–5132.
- 34. Labrecque LG, Xue SA, Kazembe P, Phillips J, Lampert I, et al. (1999) Expression of Epstein-Barr virus lytically related genes in African Burkitt's lymphoma: correlation with patient response to therapy. Int J Cancer 81: 6–11.
- 35. Tang W, Harmon P, Gulley ML, Mwansambo C, Kazembe PN, et al. (2010) Viral response to chemotherapy in endemic Burkitt lymphoma. Clin Cancer Res 16: 2055–2064.
- 36. Feng WH, Hong G, Delecluse HJ, Kenney SC (2004) Lytic induction therapy for Epstein-Barr virus-positive B-cell lymphomas. J Virol 78: 1893–1902.
- 37. Chan AT, Tao Q, Robertson KD, Flinn IW, Mann RB, et al. (2004) Azacitidine induces demethylation of the Epstein-Barr virus genome in tumors. J Clin Oncol 22: 1373–1381.
- 38. Skibola CF, Curry JD, Nieters A (2007) Genetic susceptibility to lymphoma. Haematologica 92: 960–969.
- 39. Rothman N, Skibola CF, Wang SS, Morgan G, Lan Q, et al. (2006) Genetic variation in TNF and IL10 and risk of non-Hodgkin lymphoma: a report from the InterLymph Consortium. Lancet Oncol 7: 27–38.
- 40. Lan Q, Zheng T, Rothman N, Zhang Y, Wang SS, et al. (2006) Cytokine polymorphisms in the Th1/Th2 pathway and susceptibility to non-Hodgkin lymphoma. Blood 107: 4101–4108.
- 41. Berglund M, Thunberg U, Roos G, Rosenquist R, Enblad G (2005) The interleukin-10 gene promoter polymorphism (−1082) does not correlate with clinical outcome in diffuse large B-cell lymphoma. Blood 105: 4894–4895.
- 42. Kube D, Hua TD, von Bonin F, Schoof N, Zeynalova S, et al. (2008) Effect of interleukin-10 gene polymorphisms on clinical outcome of patients with aggressive non-Hodgkin's lymphoma: an exploratory study. Clin Cancer Res 14: 3777–3784.
- 43. Mörmann M, Rieth H, Hua TD, Assohou C, Roupelieva M, et al. (2004) Mosaics of gene variations in the Interleukin-10 gene promoter affect interleukin-10 production depending on the stimulation used. Genes Immun 5: 246–255.
- 44. Suárez A, Castro P, Alonso R, Mozo L, Gutiérrez C (2003) Interindividual variations in constitutive interleukin-10 messenger RNA and protein levels and their association with genetic polymorphisms. Transplantation 75: 711–717.
- 45. Eskdale J, Gallagher G, Verweij CL, Keijsers V, Westendorp RG, et al. (1998) Interleukin 10 secretion in relation to human IL-10 locus haplotypes. Proc Natl Acad Sci USA 95: 9465–9470.
- 46. Rousset F, Garcia E, Defrance T, Péronne C, Vezzio N, et al. (1992) Interleukin 10 is a potent growth and differentiation factor for activated human B lymphocytes. Proc Natl Acad Sci USA 89: 1890–1893.
- 47. Ogden CA, Pound JD, Batth BK, Owens S, Johannessen I, et al. (2005) Enhanced apoptotic cell clearance capacity and B cell survival factor production by IL-10-activated macrophages: implications for Burkitt's lymphoma. J Immunol 174: 3015–3023.
- 48. Suarez-Kurtz G, Pena SDJ (2007) Pharmacogenetic studies in the Brazilian population. In: Pharmacogenomics in Admixed Populations. Suarez-Kurtz G, editor. Austin, TX: Landes Bioscience. pp. 75–98.
- 49. Suarez-Kurtz G, Vargens DD, Struchiner CJ, Bastos-Rodrigues L, Pena S DJ (2007) Self-reported skin color, genomic ancestry and the distribution of GST polymorphisms. Pharmacogenet. Genomics 17: 765–771.
- 50. Suarez-Kurtz G (2010) Pharmacogenetics in the Brazilian population. Front Pharmacol 1: 118.
- 51. Patte C (2002) Treatment of mature B-ALL and high grade B-NHL in children. Best Pract Res Clin Haematol 15: 695–711.